1. Transfer of a catabolic pathway for chloromethane in Methylobacterium strains highlights different limitations for growth with chloromethane or with dichloromethane
- Author
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Joshua Michener, Stéphane Vuilleumier, Francoise Bringel, Christopher J. Marx, Génétique moléculaire, génomique, microbiologie (GMGM), Université de Strasbourg (UNISTRA)-Centre National de la Recherche Scientifique (CNRS), Massachusetts Institute of Technology. Department of Biological Engineering, Michener, Joshua K, and Centre National de la Recherche Scientifique (CNRS)-Université de Strasbourg (UNISTRA)
- Subjects
0301 basic medicine ,Microbiology (medical) ,030106 microbiology ,lcsh:QR1-502 ,Microbiology ,Chloride ,lcsh:Microbiology ,microbial evolution ,03 medical and health sciences ,chemistry.chemical_compound ,bioremediation ,Methylobacterium extorquens ,medicine ,horizontal gene transfer (HGT) ,ComputingMilieux_MISCELLANEOUS ,Original Research ,Dichloromethane ,biology ,Catabolism ,Chloromethane ,chloromethane ,biology.organism_classification ,3. Good health ,030104 developmental biology ,chemistry ,Biochemistry ,Horizontal gene transfer ,Methylobacterium ,Heterologous expression ,medicine.drug ,[SDV.EE.IEO]Life Sciences [q-bio]/Ecology, environment/Symbiosis - Abstract
Chloromethane (CM) is an ozone-depleting gas, produced predominantly from natural sources, that provides an important carbon source for microbes capable of consuming it. CM catabolism has been difficult to study owing to the challenging genetics of its native microbial hosts. Since the pathways for CM catabolism show evidence of horizontal gene transfer, we reproduced this transfer process in the laboratory to generate new CM-catabolizing strains in tractable hosts. We demonstrate that six putative accessory genes improve CM catabolism, though heterologous expression of only one of the six is strictly necessary for growth on CM. In contrast to growth of Methylobacterium strains with the closely related compound dichloromethane (DCM), we find that chloride export does not limit growth on CM and, in general that the ability of a strain to grow on DCM is uncorrelated with its ability to grow on CM. This heterologous expression system allows us to investigate the components required for effective CM catabolism and the factors that limit effective catabolism after horizontal transfer., National Institutes of Health (U.S.) (Grant F32 GM106629)
- Published
- 2016