1. Taurine inhibits serum deprivation-induced osteoblast apoptosis via the taurine transporter/ERK signaling pathway
- Author
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Youwen Deng, Jing Li, Zhengguang Wang, Bangliang Yin, Lei-Yi Zhang, Yawei Li, Yue-Ying Zhou, Dong-Zhe Li, Guohua Lv, Fei Chen, Bing Wang, and Weidong Liu
- Subjects
MAPK/ERK pathway ,medicine.medical_specialty ,Taurine ,Physiology ,Immunology ,Biophysics ,Apoptosis ,Biology ,Biochemistry ,chemistry.chemical_compound ,Mice ,Internal medicine ,medicine ,Extracellular ,Animals ,RNA, Messenger ,General Pharmacology, Toxicology and Pharmaceutics ,Extracellular Signal-Regulated MAP Kinases ,lcsh:QH301-705.5 ,Analysis of Variance ,lcsh:R5-920 ,Membrane Glycoproteins ,Osteoblasts ,Kinase ,Caspase 3 ,General Neuroscience ,Cytochrome c ,Membrane Transport Proteins ,Osteoblast ,Cell Biology ,General Medicine ,3T3 Cells ,In vitro ,Caspase 9 ,ERK ,medicine.anatomical_structure ,Endocrinology ,chemistry ,lcsh:Biology (General) ,biology.protein ,Cattle ,Taurine transporter ,lcsh:Medicine (General) - Abstract
Taurine has positive effects on bone metabolism. However, the effects of taurine on osteoblast apoptosis in vitro have not been reported. The aim of this study was to investigate the activity of taurine on apoptosis of mouse osteoblastic MC3T3-E1 cells. The data showed that 1, 5, 10, or 20 mM taurine resulted in 16.7, 34.2, 66.9, or 63.7% reduction of MC3T3-E1 cell apoptosis induced by the serum deprivation (serum-free α-MEM), respectively. Taurine (1, 5, or 10 mM) also reduced cytochrome c release and inhibited activation of caspase-3 and -9, which were measured using fluorogenic substrates for caspase-3/caspase-9, in serum-deprived MC3T3-E1 cells. Furthermore, taurine (10 mM) induced extracellular signal-regulated kinase (ERK) phospho-rylation in MC3T3-E1 cells. Knockdown of the taurine transporter (TAUT) or treatment with the ERK-specific inhibitor PD98059 (10 μM) blocked the activation of ERK induced by taurine (10 mM) and abolished the anti-apoptotic effect of taurine (10 mM) in MC3T3-E1 cells. The present results demonstrate for the first time that taurine inhibits serum deprivation-induced osteoblast apoptosis via the TAUT/ERK signaling pathway. Key words: Taurine; Osteoblasts; Apoptosis; Taurine transporter; ERK
- Published
- 2011