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Your search keyword '"Magdalena Wolczyk"' showing total 3 results
Start Over Author "Magdalena Wolczyk" Topic biochemistry
3 results on '"Magdalena Wolczyk"'

1. TIAR and FMRP shape pro-survival nascent proteome of leukemia cells in the bone marrow microenvironment

Author

Magdalena Wolczyk, Remigiusz Serwa, Agata Kominek, Agata Klejman, Jacek Milek, Marta Chwałek, Laura Turos-Korgul, Agata Charzyńska, Michal Dabrowski, Magdalena Dziembowska, Tomasz Skorski, Katarzyna Piwocka, and Paulina Podszywalow-Bartnicka

Subjects
Biochemistry, Molecular biology, Cancer, Proteomics, Science
Abstract

Summary: Chronic myeloid leukemia (CML) cells circulate between blood and bone marrow niche, representing different microenvironments. We studied the role of the two RNA-binding proteins, T-cell-restricted intracellular antigen (TIAR), and the fragile X mental retardation protein (FMRP) in the regulation of protein translation in CML cells residing in settings mimicking peripheral blood microenvironment (PBM) and bone marrow microenvironment (BMM). The outcomes showed how conditions shaped the translation process through TIAR and FMRP activity, considering its relevance in therapy resistance. The QuaNCAT mass-spectrometric approach revealed that TIAR and FMRP have a discrete modulatory effect on protein synthesis and thus affect distinct aspects of leukemic cells functioning in the hypoxic niche. In the BMM setup, FMRP impacted metabolic adaptation of cells and TIAR substantially supported the resistance of CML cells to translation inhibition by homoharringtonine. Overall, our results demonstrated that targeting post-transcriptional control should be considered when designing anti-leukemia therapeutic solutions.

Published
2023
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3. Stress granules assembly affects detection of mRNA in living cells by the NanoFlares; an important aspect of the technology

Author

Katarzyna Piwocka, Paulina Podszywalow-Bartnicka, Lukasz Bugajski, and Magdalena Wolczyk

Subjects
0301 basic medicine, Cell, Biophysics, RNA-binding protein, Biology, Genes, abl, Cytoplasmic Granules, Biochemistry, Fluorescence, Flow cytometry, Cell Line, 03 medical and health sciences, Mice, Stress granule, medicine, Protein biosynthesis, Animals, RNA, Messenger, Molecular Biology, Messenger RNA, medicine.diagnostic_test, BRCA1 Protein, RNA, RNA-Binding Proteins, Molecular biology, Cell biology, 030104 developmental biology, medicine.anatomical_structure, Cell culture, Protein Biosynthesis
Abstract

The recently announced new methodologies to detect mRNA molecules in single cells offer opportunities for research, medicine and molecular diagnostics. The NanoFlare RNA Detection Probes are tools for characterizing RNA content (not localization) using fluorescence-based approaches in living cells. Combined with flow cytometry, NanoFlares have expanded the available possibilities of quantitative analysis of mRNA level in a single cell. Herein we present that in some cases, the specific NanoFlare probes (SmartFlares) detect different amounts of mRNA compared to qPCR. Using the previously published model, in which we studied influence of BCR-ABL oncogene on BRCA1 mRNA translation, we found that the NanoFlare-mediated measurement of mRNA was affected by the assembly of stress granules, structures which store mRNA in complexes with RNA binding proteins. With the usage of chemical compounds we confirmed that under conditions supporting assembly of stress granules, the detection of mRNAs by these probes was decreased, whereas disassembly resulted in the increased mRNAs detection. Altogether, we showed that assembly of stress granules could interfere with mRNA accessibility to the NanoFlare RNA Detection Probes, indicating that the SmartFlares could recognize only the translationally active pool of mRNA, contrary to qPCR. This can significantly influence the quality of obtained data and should be taken into consideration while planning the analysis of mRNA markers using NanoFlares.

Published
2016

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