1. Sicyos angulatus ameliorates acute liver injury by inhibiting oxidative stress via upregulation of anti-oxidant enzymes
- Author
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Hong-Sun Yook, Yong-Hoon Kim, Jung-Ran Noh, Chul-Ho Lee, Dong-Hee Choi, Won-Keon Oh, Hyun-Yong Kim, Jung Hwan Hwang, Kyoung-Shim Kim, Jin-Pyo An, and Sung-Je Moon
- Subjects
0301 basic medicine ,Male ,extracts ,Physiology ,injury ,Clinical Biochemistry ,Sicyos angulatus ,Pharmacology ,medicine.disease_cause ,liver ,Biochemistry ,Loranthaceae ,Antioxidants ,03 medical and health sciences ,Mice ,Downregulation and upregulation ,tert-Butylhydroperoxide ,medicine ,lcsh:Pathology ,Animals ,oxidative stress ,Aspartate Aminotransferases ,lcsh:QH301-705.5 ,Acute liver injury ,chemistry.chemical_classification ,reactive oxygen species ,Reactive oxygen species ,Glutathione Peroxidase ,biology ,Plant Extracts ,Superoxide Dismutase ,Biochemistry (medical) ,apoptosis ,Alanine Transaminase ,Cell Biology ,Anti oxidant ,biology.organism_classification ,Glutathione ,Mice, Inbred C57BL ,enzyme ,030104 developmental biology ,Enzyme ,chemistry ,lcsh:Biology (General) ,Apoptosis ,Lipid Peroxidation ,Oxidative stress ,Research Article ,lcsh:RB1-214 - Abstract
Objective: We aimed to investigate the effect of Sicyos angulatus (SA) ethanolic extracts as antioxidants and potential treatments for liver disease. Methods: To establish a mouse model of liver injury, C57BL/6 male mice were injected via the caudal vein with a single dose of concanavalin A (Con A, 15 mg kgā1). SA extracts were administered once by oral gavage 30 min before Con A injection. Results:In vitro studies showed that SA decreased tert-butyl hydroperoxide (t-BHP)-induced reactive oxygen species (ROS) production. SA administration reduced plasma alanine aminotransferase (ALT) and aspartate aminotransferase (AST) levels, as well as hepatic ROS levels, in a dose-dependent manner. Moreover, SA increased the activities of the hepatic antioxidant enzymes superoxide dismutase, catalase, and glutathione peroxidase in a dose-dependent manner. Furthermore, SA treatment reduced pro-apoptotic protein levels. Con A-mediated cytosolic release of Smac/DIABLO and apoptosis-inducing factor (AIF), which are markers of necrosis, were dramatically decreased in HepG2 cells treated with SA. Conclusion: SA ameliorated liver injury and might be a good strategy for the treatment of liver injury.
- Published
- 2018