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Your search keyword '"James B. Powell"' showing total 5 results
Start Over Author "James B. Powell" Topic biochemistry (medical)
5 results on '"James B. Powell"'

1. Automated Procedure for Assay of 2,3-Diphosphoglycerate in Red Cells by Measuring Enzymatically Released Inorganic Phosphorus

Author

James B. Powell, Clarence E. Emery, and Gaylon A. Peyton

Subjects
Phosphoglycerate mutase, Chromatography, Biochemistry, Chemistry, Biochemistry (medical), Clinical Biochemistry, Phosphatase, Diphosphoglycerate, Inorganic phosphorus, Automated technique
Abstract

An automated technique is reported for measuring erythrocyte 2,3-diphosphoglycerate (DPG). We have automated a specific method, in which the DPG phosphatase activity of phosphoglycerate mutase is used and potentiated by phosphoglycolate. Precision, accuracy, recovery, and comparability with a reference method are discussed.

Published
1972
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3. A mechanized screening procedure for sickling hemoglobins

Author

Jean E. Matusik, David M. Gregory, and James B. Powell

Subjects
Hemoglobin s, Autoanalysis, Chromatography, Screening test, Chemistry, Hemoglobins, Abnormal, Biochemistry (medical), Clinical Biochemistry, Phosphate buffered saline, Anemia, Sickle Cell, General Medicine, Biochemistry, Phosphates, Solubility, Reagent, Humans, Densitometry
Abstract

A turbidimetric test for hemoglobin S and other sickling hemoglobins has been adapted to the Auto-Analyzer. This procedure utilizes the lack of solubility of sickling hemoglobins in a phosphate buffer reagent. It is inexpensive and the results are comparable to those of other screening tests.

Published
1972
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4. Rapid Solubility Test for Detection of Hemoglobin S

Author

Jean E. Matusik, James B. Powell, and David M. Gregory

Subjects
Hemoglobin s, Rapid screening test, Chromatography, Chemistry, Biochemistry (medical), Clinical Biochemistry, Phosphate buffered saline, Solubility test, Suspension (vehicle)
Abstract

We have devised a rapid screening test for use in detecting sickling hemoglobins. This test depends on the insolubility of sickling hemoglobins in 2.24 molar phosphate buffer. Sickling hemoglobins form a turbid suspension in this test, in which positive and negative results, respectively, appear very much like those for the "Sickledex" test.

Published
1971
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