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Carboxymethyl cellulose derivatives bearing tetrabutylammonium moieties (CMC-TBA) were synthesized by the acidification of carboxymethyl cellulose (CMC) followed by acid-base neutralization with tetrabutylammonium hydroxide. The products were identified by Fourier transform infrared (FT-IR), 1 H nuclear magnetic resonance (NMR) spectroscopy and the degrees of substitution (DS) values were also quantified according to the integral area values in 1 H NMR spectra. It was revealed that DS values had a positive relationship with the molar ratios of TBAOH to CMC. The antibacterial behaviors against gram-positive bacteria S. aureus and gram-negative bacteria E. coli were investigated using serial two-fold dilution method (MIC and MBC) and the disc diffusion method (inhibition zone). The results showed that comparison with CMC, all new CMC-TBA derivatives exhibited high antibacterial activity that depends on bacteria type and their degrees of cationization. The antibacterial action was more effective against S. aureus than E. coli, which could be attributed to the fact that the latter has a complicated bilayer structure of cell wall. Besides, an apparent tendency that the antibacterial activity of CMC-TBA derivatives enhanced with an increase in the degrees of cationization was found. This work suggests that these new derivatives can be introduced as efficient antibacterial biomaterials for biomedical purposes., Competing Interests: Declaration of competing interest All authors declared no conflict of interest., (Copyright © 2021 Elsevier B.V. All rights reserved.)

Acetobacter xylinum produces highly crystalline cellulose extracellularly using glucose as a carbon source. The polymer formed is free of other biogenic compounds, separable in a simple way and characterized by its high water-absorption capacity. Stepwise solvent exchange from water to unpolar solvents leads to a drastic decrease of the water content of the bacterial cellulose without decrease of the highly swollen and activated state. Heterogeneous as well as homogeneous derivatizations, e.g. carboxymethylation, silylation and acetylation, were performed on the wet or dried biopolymer. Furthermore, different methods for formation of hollow fibres during biosynthesis were investigated. Such tubes may have applications as biocompatible material in medicine.