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To ascertain absorption of arsenate and phenanthrene as well as their influence on carbon groups in excised roots of

A pot experiment was carried out to study the effects of soil moisture on the growth and arsenic uptake of As-hyperaccumulator Pteris vittata L. The results showed that the remediation efficiency of As was the highest when the soil moisture was between 35%-45%. P. vittata grew best under 45% water content, and its aboveground and underground plant dry weights were 2.95 g x plant(-1) and 11.95 g x plant(-1), respectively; the arsenic concentration in aboveground and roots was the highest under 35% water content, and 40% content was the best for accumulation of arsenic in P. vittata. Moreover, controlling the soil moisture to 35%-45% enhanced the conversion of As(V) to As(III) in aboveground plant, and promoted arsenic detoxification in P. vittata. These above results showed that soil moisture played an important role in the absorption and transport of arsenic by P. vittata. The results of this study can provide important guidance for the large-scale planting of P. vittata and the moisture management measures in engineering application.

A pot experiment was carried out to study the root character and As accumulation of Pteris vittata L. affected by actinomycete PSQ, shf2 and bacteria Ts37, C13. The results indicated that growth of the fern was improved by the microorganisms. The biomass, root activity and root volume of shf2 treatment were 11.5 g/pot, 2.01 microg/(g x h), 38.3 mL, which were higher than those of other microorganisms treatments. Arsenic concentrations in the plants treated by the microorganisms were higher than that of the control treatment. The As concentration of leaves in Ts37 treatment was up to 837 mg/kg, 206% more than that of the control. The As concentration of root treated by shf2 is 427 mg/kg, 88% more than that of the control. The arsenic accumulation by the plant in microorganism treatments was higher than that of the control, especially shf2 treatment up to 5804 microg/pot, 136% more than that of the control. The phytoremediation efficiency of the fern in greenhouse experiment after 45d was from 8.9% to 11.3%. The ability of As-accumulation of Pteris vittata is greatly enhanced by application of microorganism, and actinomycete shf2 is proved as the perfect one.

Fertilization has become one of the essential measures for enhancing efficiency of phytoremediating contaminated soils with heavy metal. In order to screen optimal P fertilizer for As-phytoremediation, a greenhouse study was conducted on the growth, As-accumulation and uptake of N, P and K by Pteris vittata L. in As-contaminated soils with different forms of P fertilizers. The results indicated that the biomass of plant with As addition decreased compared to no As-addition treatments except fused calcium magnesium phosphate (CaMg-P) treatment. The plants in As addition soils with CaMg-P, calcium dihydrogen phosphate (CDP) and di-ammonium phosphate (DAP) had higher biomass than those with other P fertilizer and control (0.83 g/pot). The As accumulations of plant aboveground in As addition soils are in order of CDPCaMg-PDAPPotassium Phosphate MonobasicMonosodium phosphatecontrolCalcium superphosphate. The efficiency of As removal from As addition soils with CDP was the highest, 7.28%. Thus it can be seen the ability of phytoremediation using P. vittata could be improved by P fertilization, which CDP should be recommended preferentially and CaMg-P and DAP is considered as replaceable fertilizer for sake of pH, N, P and available As in phytoremediated soils.