8 results on '"Ribeiro, Ana R."'
Search Results
2. Chiral pharmaceuticals in the environment
- Author
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Ribeiro, Ana R., Castro, Paula M. L., and Tiritan, Maria E.
- Published
- 2012
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3. Chiral Analysis of Pesticides and Drugs of Environmental Concern: Biodegradation and Enantiomeric Fraction
- Author
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Maia, Alexandra S., Ribeiro, Ana R., Castro, Paula M. L., Tiritan, Maria Elizabeth, CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, and Veritati - Repositório Institucional da Universidade Católica Portuguesa
- Subjects
Physics and Astronomy (miscellaneous) ,Agrochemical ,General Mathematics ,Fraction (chemistry) ,Enantioselectivity ,010501 environmental sciences ,pharmaceuticals ,01 natural sciences ,biodegradation ,enantioselectivity ,Enantiomeric fraction (EF) ,Computer Science (miscellaneous) ,Pesticides ,enantiomeric fraction (EF) ,0105 earth and related environmental sciences ,Pollutant ,Chemistry ,business.industry ,organic chemicals ,chiral analysis ,lcsh:Mathematics ,010401 analytical chemistry ,Enantioselective synthesis ,pesticides ,Pesticide ,Biodegradation ,Chiral analysis ,lcsh:QA1-939 ,0104 chemical sciences ,13. Climate action ,Chemistry (miscellaneous) ,Environmental chemistry ,Pharmaceuticals ,Enantiomer ,Ecotoxicity ,business - Abstract
The importance of stereochemistry for medicinal chemistry and pharmacology is well recognized and the dissimilar behavior of enantiomers is fully documented. Regarding the environment, the significance is equivalent since enantiomers of chiral organic pollutants can also differ in biodegradation processes and fate, as well as in ecotoxicity. This review comprises designed biodegradation studies of several chiral drugs and pesticides followed by enantioselective analytical methodologies to accurately measure the enantiomeric fraction (EF). The enantioselective monitoring of microcosms and laboratory-scale experiments with different environmental matrices is herein reported. Thus, this review focuses on the importance of evaluating the EF variation during biodegradation studies of chiral pharmaceuticals, drugs of abuse, and agrochemicals and has implications for the understanding of the environmental fate of chiral pollutants. The authors wish to thank Fundação para a Ciência e Tecnologia—FCT for financial support under the project Fluoropharma PTDC/EBB-EBI/111699/2009, the PhD grant attributed to Alexandra S. Maia SFRH/BD/86939/2012, QREN-POPH, European Social Fund, MCTES, PEst FCOMP-01-0124-FEDER-022718; PEst-OE/EQB/LA0016/2011, PEst-OE/SAU/UI4040/2014. This research was partially supported by the Strategic Funding UID/Multi/04423/2013 through national funds provided by FCT—Fundação para a Ciência e Tecnologia and European Regional Development Fund (ERDF), in the framework of the program PT2020 and by CESPU (ChiralDrugs_CESPU_2017)
- Published
- 2017
- Full Text
- View/download PDF
4. Enantioselective biodegradation of pharmaceuticals, alprenolol and propranolol, by an activated sludge inoculum
- Author
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Ribeiro, Ana R., Afonso, Carlos M., Castro, Paula M. L., Tritan, Maria E., and Veritati - Repositório Institucional da Universidade Católica Portuguesa
- Subjects
Health, Toxicology and Mutagenesis ,Adrenergic beta-Antagonists ,HPLC-FD ,Propranolol ,Matrix (chemical analysis) ,Beta-blockers ,chemistry.chemical_compound ,Chirobiotic CSP ,Enantioselective biodegradation ,medicine ,Alprenolol ,Chromatography, High Pressure Liquid ,Chromatography ,Sewage ,Chemistry ,Public Health, Environmental and Occupational Health ,Enantioselective synthesis ,Stereoisomerism ,General Medicine ,Biodegradation ,Pollution ,Biodegradation, Environmental ,Activated sludge ,Chiral ecotoxicity ,Degradation (geology) ,Enantiomer ,medicine.drug - Abstract
Biodegradation of chiral pharmaceuticals in the environment can be enantioselective. Thus quantification of enantiomeric fractions during the biodegradation process is crucial for assessing the fate of chiral pollutants. This work presents the biodegradation of alprenolol and propranolol using an activated sludge inoculum, monitored by a validated enantioselective HPLC method with fluorescence detection. The enantioseparation was optimized using a vancomycin-based chiral stationary phase under polar ionic mode. The method was validated using a minimal salts medium inoculated with activated sludge as matrix. The method was selective and linear in the range of 10-800ng/ml, with a R2>0.99. The accuracy ranged from 85.0 percent to 103 percent, the recovery ranged from 79.9 percent to 103 percent, and the precision measured by the relative standard deviation (RSD) was
- Published
- 2013
5. Microbial degradation of 17beta -estradiol and 17alpha -ethinylestradiol followed by a validated HPLC-DAD method
- Author
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Ribeiro, Ana R., Carvalho, Maria F., Afonso, Carlos M. M., Tiritan, Maria E., Castro, Paula M.L., and Veritati - Repositório Institucional da Universidade Católica Portuguesa
- Subjects
Estrone ,Metabolite ,Chryseobacterium ,HPLC-DAD ,Ethinyl Estradiol ,chemistry.chemical_compound ,Sphingobacterium ,Alcaligenes ,Endocrine disrupting chemicals ,Microbial biodegradation ,Chromatography, High Pressure Liquid ,Chromatography ,17α-ethinylestradiol ,biology ,Estradiol ,Sewage ,Pseudomonas ,17β-estradiol ,Estrogens ,General Medicine ,Microbial consortium ,Biodegradation ,biology.organism_classification ,Pollution ,Activated sludge ,Biodegradation, Environmental ,chemistry ,Gram-Negative Aerobic Rods and Cocci ,Food Science - Abstract
This work aimed at studying the biodegradation of two estrogens, 17alpha -estradiol (E2) and 17beta -ethinylestradiol (EE2), and their potential metabolism to estrone (E1) by microbial consortia. The biodegradation studies were followed by High Performance Liquid Chromatography-Diode Array Detector (HPLC-DAD) using a specifically developed and validated method. Biodegradation studies of the estrogens (E2 and EE2) were carried out with activated sludge (consortium A, CA) obtained from a Wastewater Treatment Plant (WWTP) and with a microbial consortium able to degrade recalcitrant compounds, namely fluorobenzene (consortium B, CB). E2 was more extensively degraded than EE2 by CA whereas CB was only able to degrade E2. The addition of acetate as a supplementary carbon source led to a faster biodegradation of E2 and EE2. E1 was detected as a metabolite only during the degradation of E2. The 16S rRNA gene sequence analyses of strains recovered from the degrading cultures revealed the presence of the genera Pseudomonas, Chryseobacterium and Alcaligenes. The genera Pseudomonas and Chryseobacterium were retrieved from cultures supplied with E2 and EE2, while the genus Alcaligenes was found in the presence of E2, suggesting that they might be involved in the degradation of these compounds.
- Published
- 2010
6. Enantioselective biodegradation of fluoxetine by the bacterial strain Labrys portucalensis F11.
- Author
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Moreira, Irina S., Ribeiro, Ana R., Afonso, Carlos M., Tiritan, Maria E., and Castro, Paula M. L.
- Subjects
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BIODEGRADATION , *FLUOXETINE , *CHIRALITY , *FLUORINATION , *MENTAL depression , *THERAPEUTICS , *STOICHIOMETRY - Abstract
Fluoxetine (FLX) is a chiral fluorinated pharmaceutical indicated mainly for the treatment of depression and is one of the most dispensed drugs in the world. There is clear evidence of environmental contamination with this drug and its active metabolite norfluoxetine (NFLX). In this study the enantioselective biodegradation of racemic FLX and of its enantiomers by Labrys portucalensis strain F11 was assessed. When 2 μM of racemic FLX was supplemented as sole carbon source, complete removal of both enantiomers, with stoichiometric liberation of fluoride, was achieved in 30 d. For racemic FLX concentration of 4 and 9 μM, partial degradation of the enantiomers was obtained. In the presence of acetate as an additional carbon source, at 4, 9 and 21 μM of racemic FLX and at 25 μM of racemic FLX, (S)-FLX or (R)-FLX, complete degradation of the two enantiomers occurred. At higher concentrations of 45 and 89 μM of racemic FLX, partial degradation was achieved. Preferential degradation of the (R)-enantiomer was observed in all experiments. To our knowledge, this is the first time that enantioselective biodegradation of FLX by a single bacterium is reported. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
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7. Degradation of fluoroquinolone antibiotics and identification of metabolites/transformation products by liquid chromatography–tandem mass spectrometry.
- Author
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Maia, Alexandra S., Ribeiro, Ana R., Amorim, Catarina L., Barreiro, Juliana C., Cass, Quezia B., Castro, Paula M.L., and Tiritan, Maria Elizabeth
- Subjects
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FLUOROQUINOLONES , *ANTIBIOTICS , *BIODEGRADATION , *METABOLITES , *LIQUID chromatography-mass spectrometry , *MOLECULAR structure , *INTERMEDIATES (Chemistry) - Abstract
Highlights: [•] The multi-techniques analytical approach allowed the evaluation of the degradation of fluoroquinolones by a microbial consortium. [•] Fluoride ions released exposed the incomplete mineralization of the fluoroquinolones. [•] Metabolites were identified by LC–MS/MS and QqTOF MS. [•] New structures of the intermediates were proposed. [Copyright &y& Elsevier]
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- 2014
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8. Microbial degradation of pharmaceuticals followed by a simple HPLC-DAD method.
- Author
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Ribeiro, Ana R., Gonçalves, Virgínia M.F., Maia, Alexandra S., Carvalho, Maria F., Castro, Paula M.L., and Tiritan, Maria E.
- Subjects
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BIODEGRADATION , *HIGH performance liquid chromatography , *ANTIBIOTICS , *TARGETED drug delivery , *TRIFLUOROACETIC acid , *ACTIVATED sludge process , *ANTI-inflammatory agents , *DETECTORS , *AROMATIC fluorine compounds , *CARBAMAZEPINE - Abstract
The biodegradation of five pharmaceutical ingredients (PIs) of different therapeutic classes, namely antibiotics (trimethoprim, sulfametoxazole and ciprofloxacin), anti-inflammatory (diclofenac) and anti-epileptic (carbamazepine), by two distinct microbial consortia, was investigated. For the monitoring of biodegradation assays, a simple HPLC-DAD (High Performance Liquid Chromatography – Diode Array Detector) method was developed and validated. The separation of the target pharmaceuticals was performed using an environmental friendly mobile phase in a gradient mode of 0.1% triethylamine (TEA) in water acidified at pH 2.23 with trifluoroacetic acid (TFAA) and ethanol as organic solvent. The method revealed to be selective, linear and precise in the range of 1.0 to 30.0 μg/mL for all PIs. Biodegradation assays were performed using activated sludge and a bacterial consortium (able to degrade fluoroaromatic compounds) supplemented with the target PIs at a final concentration of 25 μg/mL. The results revealed that activated sludge removed the target compounds more efficiently than the bacterial consortium. [ABSTRACT FROM AUTHOR]
- Published
- 2012
- Full Text
- View/download PDF
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