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Laccase activity secreted by Pleurotus eryngii Han 1787 and Lentinus edodes Han 1788 grown on six types of agroindustrial wastes was investigated. Maximum laccase activity of P. eryngii Han 1787 on Ulmus pumila, Juniperus formosana, Pinus tabuliformis, cottonseed shell, corncob, and leaf of corncob was nearly 5.77-fold, 2.37-fold, 2.78-fold, 2.81-fold, 11.53-fold, and 6.73-fold higher than that of L. edodes Han1788 on corresponding agroindustrial wastes. In general, the capacity of secreting laccase of P. eryngii Han 1787 was superior to that of L. edodes Han 1788. Furthermore, laccase activity of P. eryngii Han 1787 on the leaf of corncob, the corncob, Ulmus pumila, and Juniperus formosana was relatively stable during the whole fermentation process. Different fungi showed different preferences in different agroindustrial wastes to secret laccase on whole fermentation stage. The presence of leaf of corncob was useful for improving laccase activity of P. eryngii Han 1787, while L. edodes Han 1788 was more preferred to produce laccase along with the presence of Juniperus formosana. These results were preliminary conducive in laying the foundation for increasing industrial laccase-producing strains and producing low-cost laccase.

The laccase activity of three cultivated Pleurotus strains was investigated using different lignocellulosic biomasses for solid-state fermentation. The lignocellulosic biomasses were Sophora japonica, Salix babylonica, Populus beijingensis, and Pinus tabuliformis, which were selected because of their laccase producing ability and low cost. The maximum laccase activities from P. ostreatus Han 1189, P. citrinopileatus Han 1192, and P. eryngii Han 1205 using Sophora japonica were 768.74 U/L ± 11.25 U/L, 144.77 U/L ± 9.70 U/L, and 200.42 U/L ± 6.85 U/L, respectively. The three species of Pleurotus showed consistency in having a preference of hardwood to secret laccase under solid-state fermentation when facing hardwood and softwood as substrates. Furthermore, the presence of Sophora japonica was beneficial to improving the laccase activity for three Pleurotus strains. The capacity of the laccase secretion of P. ostreatus Han 1189 was superior to the capacity of P. citrinopileatus Han 1192 and P. eryngii Han 1205. The discovery was helpful for obtaining new high-producing strains and suitable wood materials, as well as expanding these high-producing strains for industrial applications to obtain high laccase activity, high laccase yield, and low cost laccase.