7 results on '"Wang, Hongxin"'
Search Results
2. Green Synthesis of Silver Nanoparticles Using Mushroom Flammulina velutipes Extract and Their Antibacterial Activity Against Aquatic Pathogens
- Author
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Zhang, Lizhi, Wei, Yanan, Wang, Hongxin, Wu, Fanlin, Zhao, Yancui, Liu, Xiaoli, Wu, Hongyan, Wang, Lei, and Su, Hongyan
- Published
- 2020
- Full Text
- View/download PDF
3. Regulation and mechanism of organic selenium on quorum sensing, biofilm, and antioxidant effects of Lactobacillus paracasei.
- Author
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Lou, Zaixiang, Dong, Jiale, Tao, Hongwei, Tan, Yeexuan, and Wang, Hongxin
- Subjects
QUORUM sensing ,SELENIUM ,LACTOBACILLUS ,SELENOPROTEINS ,PROTEIN kinases ,BIOFILMS ,ORGANIC products ,LACTOBACILLUS rhamnosus - Abstract
Different organic compounds can have varying degrees of impact on the activity of Lactobacillus paracasei. The study focused on the impact and action mechanism of different organic selenium products on the bioactivity of two strains of L. paracasei. The growth, antioxidant activity, extracellular polysaccharide secretion, quorum sensing (QS), and biofilm formation of the strains before and after the addition of organic selenium crude products and three organic selenium standard were evaluated. The results showed that the addition of crude organic selenium promoted the various activities of the strain. l‐selenocysteine had the strongest regulatory effect, with maximum GIM1.80 biofilm formation when it reached a critical concentration of 0.4 μg/mL; l‐selenomethionine resulted in the highest activity of the signal molecule Auto inducer‐2 of GDMCC1.155, when it reached a critical concentration of 0.4 μg/mL. The results of scanning electron microscopy demonstrated that the addition of organic selenium effectively improved the morphological structure of the two bacterial cells. Molecular docking revealed that the mechanism by which organic selenium regulates QS in Lactobacillus was achieved by binding two crucial receptor proteins (histidine protein kinase HKP and periplasmic binding protein LuxP) from specific sites. Furthermore, organic selenium products have a beneficial regulatory effect on the biological activity of L. paracasei. Overall, these findings provide a new alternative (organic selenium) for regulating the viability and beneficial activity of L. paracasei. Significance Statement: The study found that organic selenium can promote the growth, antioxidant activity, and biofilm formation of Lactobacillus by activating quorum sensing (QS). Uniquely, organic selenium can both positively and negatively regulate QS and biofilm formation of Lactobacillus, and critical concentrations have also been obtained. Furthermore, the regulating mechanism of organic selenium on the activity of Lactobacillus was revealed. This study provides a new alternative for regulating and stimulating the beneficial activity of probiotics. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
4. Green Synthesis of Silver Nanoparticles Using Mushroom Flammulina velutipes Extract and Their Antibacterial Activity Against Aquatic Pathogens
- Author
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Hongyan Su, Xiaoli Liu, Lei Wang, Hongyan Wu, Lizhi Zhang, Yancui Zhao, Fan-Lin Wu, Wang Hongxin, and Wei Yanan
- Subjects
0106 biological sciences ,Mushroom ,biology ,Chemistry ,Process Chemistry and Technology ,Vibrio parahaemolyticus ,Biofilm ,04 agricultural and veterinary sciences ,Antimicrobial ,biology.organism_classification ,040401 food science ,01 natural sciences ,Industrial and Manufacturing Engineering ,Silver nanoparticle ,Edible mushroom ,0404 agricultural biotechnology ,010608 biotechnology ,Food science ,Safety, Risk, Reliability and Quality ,Antibacterial activity ,Food Science ,Flammulina - Abstract
In recent years, the biosynthesized silver nanoparticles (AgNPs) have been projected as an alternative to traditional antibiotics for their superior antimicrobial properties and significant inhibition of the biofilm formation. Particularly, it is found that the effectiveness of AgNPs varies greatly with the biomaterial used in their synthesis. Mushrooms are natural resources for excellent antioxidants and bioactive compounds. Nevertheless, there are few reports on the application of mushrooms in AgNPs biosynthesis. In this study, AgNPs were successfully synthesized using Flammulina velutipes extract as reductants and stabilizing agents. The following analyses of UV-visible spectroscopy showed typical absorbance peak at 450 nm. Transmission electron microscope analysis indicated that the prepared AgNPs were monodispersed spheres with an average size of approximately 22 nm. X-ray diffraction analysis confirmed the face-centered cubic (fcc) crystalline structure of metallic silver. Furthermore, the synthesized AgNPs exhibited high stability and antibacterial activities against 6 aquatic pathogens. A subsequent challenge experiment showed that the prepared AgNPs significantly reduced the mortality rate of Ruditapes philippinarum clam infected with Vibrio parahaemolyticus. Notably, AgNPs treatment could inhibit the biofilm formation of the pathogen, indicating that the extracellular synthesis of AgNPs using mushroom can be developed into a novel effective antimicrobial agent to overcome the multidrug-resistant microorganisms in aquaculture. This study will open a new way for edible mushroom processing and wide application in aquaculture.
- Published
- 2020
5. Evaluation of Anti-Biofilm Capability of Cordycepin Against Candida albicans.
- Author
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Wang, Yu, Pei, Zejun, Lou, Zaixiang, and Wang, Hongxin
- Subjects
CANDIDA albicans ,GENTIAN violet ,FUNGAL growth ,MYCOSES ,POLYSACCHARIDES ,DRUG resistance - Abstract
Introduction: The opportunistic pathogen Candida albicans can form biofilms, resulting in drug resistance with great risk to medical treatment. Methodology: We investigated the ability of C. albicans to form biofilms on different materials, as well as the inhibitory and eradicating effects of cordycepin on biofilm. The action mechanism of cordycepin against biofilm was studied by crystal violet staining, XTT [2, 3-bis (2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] reduction method, phenol-sulfuric acid method, cellular superficial hydrophobicity (CSH) assay, and confocal laser scanning microscope observation. We also evaluated the acute toxicity of cordycepin in vivo. Results: The results showed facile formation of biofilms by C. albicans on polypropylene. The 50% minimum inhibitory concentration (MIC
50 ) of cordycepin was 0.062 mg/mL. A concentration of 0.125 mg/mL significantly decreased biofilm formation, metabolic activity, secretion of extracellular polysaccharides, and relative CSH. Cordycepin could inhibit biofilm formation at low concentration without affecting fungal growth. In addition, cordycepin effectively eradicated 59.14% of mature biofilms of C. albicans at a concentration of 0.5 mg/mL. For acute toxicity, the LD50 (50% of lethal dose) of cordycepin was determined as higher than 500 mg/kg for mice. Conclusion: The results of this study show that cordycepin significantly inhibited and eradicated biofilms by decreasing metabolic activity, the ratio of living cells, the hydrophobicity, and damaging the extracellular polysaccharides of biofilm. These findings should facilitate more effective application of cordycepin and suggest a new direction for the treatment of fungal infections. [ABSTRACT FROM AUTHOR]- Published
- 2021
- Full Text
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6. Anti-quorum sensing and anti-biofilm activity of Amomum tsaoko (Amommum tsao-ko Crevost et Lemarie) on foodborne pathogens.
- Author
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Rahman, Md Ramim Tanver, Lou, Zaixiang, Yu, Fuhao, Wang, Peng, and Wang, Hongxin
- Abstract
Cell-to-cell communication or quorum sensing (QS) leads to biofilm formation and causing other virulence factors which are extreme problems for food safety, biofilm related infectious diseases etc. This study evaluated the anti-QS activity of the Amomum tsaoko extract (0.5–4 mg/ml) by using Chromobacterium violaceum a biosensor strain and biofilm formation by crystal violate assay. Experimental results demonstrated that the overall yield of Amomum tsao-ko extract was 11.33 ± 0.3% (w/w). MIC for Staphylococcus aureus (Gram positive), Salmonella Typhimurium and Pseudomonas aeruginosa (Gram negative) was 1, 2 and 2 mg/ml, respectively. A concentration of 4 mg/ml extract showed highest biofilm inhibition 51.96% on S. Typhimurium when 47.06%, 45.28% were shown by S. aureus , P. aeruginosa respectively . The damage of biofilm architecture was observed by Confocal Laser Scanning Microscopy (CLSM). A level of 44.59% inhibition of violacein production was demonstrated when the dose was 4 mg/ml. Swarming motility inhibition was observed in a dose dependent manner. Taken together, the treatment of A. tsaoko extract can deliver value to food product and medicine by controlling pathogenesis. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
7. Separation and enrichment of burdock leaf components and their inhibition activity on biofilm formation of E. coli
- Author
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Lou, Zaixiang, Song, Xinyi, Hong, Ying, Wang, Hongxin, and Lin, Yiming
- Subjects
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BURDOCKS , *BIOFILMS , *ESCHERICHIA coli , *SEPARATION (Technology) , *SCANNING electron microscopy , *ETHANOL , *PHENOLS , *POLYAMIDES , *CHROMATOGRAPHIC analysis - Abstract
Abstract: Enrichment of burdock leaf components and the inhibition effect of the obtained 7 burdock leaf fractions on formation of biofilm have been evaluated for the first time. Firstly, burdock leaf fractions were obtained though separation of burdock leaf extracts by macroporous resin column chromatography, and four fractions were obtained, namely, water elution fraction, 20%, 34% and 70% ethanol eluted fractions. It was found that the 20% and 34% ethanol fractions both significantly inhibited the formation of biofilm by Escherichia coli, with IC50 of 1.10 and 1.01 mg/ml, respectively. The 70% ethanol elution fraction exhibited the strongest inhibitory activity against biofilm formation with an IC50 of 0.017 mg/ml. In order to obtain more fractions with higher inhibitory activity, polyamide resin was used to further purify the 34% ethanol elution fraction. The second chromatography separation significant improved the activity of burdock leaf fractions and the IC50 of a new fraction was 0.03 mg/ml. The phenolic contents of the fractions ranged from 7.5 to 369.7 mg/g and there was a high correlation between the anti-biofilm capacity and phenolic contents, except for 70% ethanol fraction. Scanning electron microscopy (SEM) observation showed that there was no biofilm formation for cultures treated with burdock leaf fraction, conforming the significant inhibition activity of burdock leaf fraction against the formation of biofilm. The results suggest that separation and enrichment of the active ingredients could significantly improve the activities of fractions and the obtained burdock leaf fractions provide new alternatives for control of biofilms. [Copyright &y& Elsevier]
- Published
- 2013
- Full Text
- View/download PDF
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