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Temperature can fluctuate widely between different seasons, and this may greatly impact many biological processes. However, little is known about its influence on the functioning of benthic microbial communities. Here we investigated the nutrient accumulation capability of periphytic biofilm under temperature fluctuations (17-35°C). Periphytic biofilm maintained the same nutrient accumulation capacity after experiencing the 'warming-hot-cooling' temperature fluctuation under both lab and outdoor conditions as those without temperature disturbance. In response to temperature increase, both community composition and species richness changed greatly and the increase in biodiversity was identified as being the underlying mechanism boosting the sustainable function in nutrient accumulation, indicating zero net effects of community changes. These findings provide insights into the underlying mechanisms of how benthic microbial communities adapt to temperature fluctuations to maintain nutrient accumulation capacity and elucidate that periphytic biofilm plays important roles in influencing nutrient cycling in aquatic ecosystems under temperature changes such as seasonal fluctuations.

Responses of microbial communities to nanotoxicity in aquatic ecosystems are largely unknown, particularly with respect to relationship between community dynamics and functions. Here, periphytic biofilms were selected as a model of species-rich microbial communities to elucidate their responses when exposed to titanium dioxide nanoparticles (TiO 2 -NPs). Especially, the relationships between the functions (e.g. organic matter and Cu 2+ removal) and community dynamics after long-term exposure to TiO 2 -NPs were assessed systematically. After 5days exposure to TiO 2 -NPs (5mgL -1 ), periphytic biofilms showed sustainable functions in pollutant removal and strong plasticity in defensing the toxic disturbance of TiO 2 -NPs, including photosynthesis and carbon metabolic diversity. The sustainable pollutant removal functions of periphytic biofilms were attributed to their functional redundancy. Specifically, periphytic biofilms altered their composition with cyanobacteria, Sphingobacteriia and Spirochaetes being the newly dominant taxa, and changed the carbon substrate utilization pattern to maintain high photosynthesis and metabolic rates. Moreover, extracellular polymeric substances (EPS) especially proteins were overproduced to bind the NPs and thereby reduce the nanotoxicity. The information obtained in this study may greatly help to understand the interactions between microbial community dynamics and function under NPs exposure conditions and functional redundancy is an important mechanism of periphytic biofilms to maintain sustainable functions., (Copyright © 2017 Elsevier B.V. All rights reserved.)

The application of periphytic biofilm in removing nitrogen from water is limited by the fluctuating nitrogen concentration. Here, we delineate a novel approach to enhance periphytic biofilm performance in nitrogen removal via upconversion luminescence of upconversion phosphors (UCPs). Nitrogen removal rates (14 d) in high nitrogen wastewater (26 mg/L) were significantly improved to 58.6% and 61.4% by UCPs doped with Pr 3+ and Li + and UCPs doped with Pr 3+ , respectively, and to 95.1% and 95.9% in low nitrogen surface water (2 mg/L), respectively. The stimulation of UCPs optimized the microbial community structure in the periphytic biofilms, and also resulted in good acclimation to use different carbon sources. The enhanced synergic action of cyanobacterial biomass, ratio of Gram +ve to Gram -ve bacteria and carbon source metabolic capacity contributed to the improved nitrogen removal. This novel approach is promising in nitrogen removal from wastewater and surface water with fluctuating initial nitrogen concentration., (Copyright © 2018 Elsevier Ltd. All rights reserved.)

This work studied Cu removal and response mechanisms of periphytic biofilms in a tubular bioreactor. Periphytic biofilms immobilized in a tubular bioreactor were used to remove Cu from wastewater with different Cu concentrations. Results showed that periphytic biofilms had a high removal efficiency (max. 99%) at a hydraulic retention time (HRT) of 12h under initial Cu concentrations of 2.0 and 10.0mgL -1 . Periphyton quickly adapted to Cu stress by regulating the community composition. Species richness, evenness and carbon metabolic diversity of the periphytic community increased when exposed to Cu. Diatoms, green algae, and bacteria (Gammaproteobacteria and Bacteroidia) were the dominant microorganisms and responsible for Cu removal. This study indicates that periphytic biofilms are promising in Cu removal from wastewater due to their strong adaptation capacity to Cu toxicity and also provides valuable information for understanding the relationships between microbial communities and heavy metal stress., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

In this study, a kind of microbial aggregates: periphytic biofilms were used for Cu removal and immobilized onto fiber for developing a novel bioreactor. Results show that periphyton can effectively entrap Cu at initial concentrations of 2-20mgL -1 due to the overproduction of EPS and porous structure of periphyton, and biosorption was the primary mechanism of Cu removal. Cu (mainly Cu 3 (OH) 4 2+ , Cu 2 (OH) 2 ) adsorption onto periphytic biofilms followed the pseudo-second order kinetic model. The biosorption process fitted the Freundlich, Langmuir and Dubinin-Radushkevich Isotherm models well and was thermodynamically spontaneous. The fiber substrate used in the periphyton bioreactor greatly increased the Cation Exchange Capacity (CEC) of the system. This study indicates that immobilization of periphytic biofilms onto fiber for novel bioreactor development is a feasible way of entrapping high-concentration Cu from wastewater.2+ and Cu 2+ ) adsorption onto periphytic biofilms followed the pseudo-second order kinetic model. The biosorption process fitted the Freundlich, Langmuir and Dubinin-Radushkevich Isotherm models well and was thermodynamically spontaneous. The fiber substrate used in the periphyton bioreactor greatly increased the Cation Exchange Capacity (CEC) of the system. This study indicates that immobilization of periphytic biofilms onto fiber for novel bioreactor development is a feasible way of entrapping high-concentration Cu from wastewater., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Upconversion phosphors (UCPs) can convert visible light into luminescence, such as UV, which can regulate the growth of microbes. Based on these fundamentals, the community composition of periphytic biofilms stimulated by UCPs doped with Pr 3+ -Li + was proposed to augment the removal of phosphorus (P) and copper (Cu). Results showed that the biofilms with community composition optimized by UCPs doped with Pr 3+ -Li + had high P and Cu 2+ removal rates. This was partly due to overall bacterial and algal abundance and biomass increases. The synergistic actions of algal, bacterial biomass and carbon metabolic capacity in the Pr-Li stimulated biofilms facilitated the removal of P and Cu 2+ . The results show that the stimulation of periphytic biofilms by lanthanide-doped UCPs is a promising approach for augmenting P and Cu 2+ removal., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Low nutrient utilization efficiency in agricultural ecosystems is the main cause of nonpoint source (NPS) pollution. Therefore, novel approaches should be explored to improve nutrient utilization in these ecosystems. Periphytic biofilms composed of microalgae, bacteria and other microbial organisms are ubiquitous and form a 'third phase' in artificial wetlands such as paddy fields. Periphytic biofilms play critical roles in nutrient transformation between the overlying water and soil/sediment, however, their contributions to nutrient utilization improvement and NPS pollution control have been largely underestimated. This mini review summarizes the contributions of periphytic biofilms to nutrient transformation processes, including assimilating and storing bioavailable nitrogen and phosphorus, fixing nitrogen, and activating occluded phosphorus. Future research should focus on augmenting the nitrogen fixing, phosphate solubilizing and phosphatase producing microorganisms in periphytic biofilms to improve nutrient utilization and thereby reduce NPS pollution production in artificial and natural wetland ecosystems., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

Allelopathy among aquatic organisms, especially microorganisms, has received growing attention in recent years for its role in shaping interactions with bloom-forming algae. Many studies have shown that allelopathy occurs and increases under nutrient limiting conditions. However, to date there is no reported direct evidence to indicate that allelopathy occurs under the condition of constant high nutrient supply. Here we report the allelopathic action of periphytic biofilm on bloom-forming cyanobacteria (Microcystis aeruginosa), which was triggered by the stress of high nutrient conditions, and continues while nutrients are maintained at high levels (trophic state index at 159 and 171). The experimental evidence indicates that the electron transport from photosystem II (PS II) to photosystem I (PS I) in M. aeruginosa is interrupted by the identified allelochemicals, (9Z)-Octadec-9-enoic acid and (9Z)-Hexadec-9-enoic acid, leading to the failure of photosynthesis and the subsequent death of M. aeruginosa. Our findings indicate that the nutrient stress of constant high nutrient supply may be a newly recognized trigger causing allelopathy between microbial competitors, and therefore opening a new direction for the better management of ecological processes in cyanobacteria-dominated and hyper-eutrophic waters.

The insufficient supply of electron donor in surface water contaminated with nitrate leads to its incomplete reduction in natural or constructed wetlands. Although the addition of organic matter (represented as chemical oxygen demand, COD) can stimulate N removal by denitrification, direct supplementation of COD creates unacceptable risks to effluent quality. An alternative for stimulating denitrification is supplying hydrogen gas (H 2 ) as an inorganic electron donor. We evaluate an innovative means to do H 2 -based denitrification of surface waters in a wetland setting: the in-situ membrane biofilm reactor (isMBfR), in which H 2 is delivered to a biofilm of denitrifying bacteria on demand based on the presence of nitrate. We carried out a proof-of-concept study in which an upper "photo zone" and a lower "MBfR root zone" were combined to remove nitrate and COD from simulated surface water. Employing mass-balances for H 2 , COD, nitrate, and oxygen, we documented nearly complete removals of nitrate and COD, except when the H 2 supply was intentionally shut off. All nitrate removal was accomplished in the "MBfR root zone," where H 2 delivery supplemented the COD supply (as needed) and provided the large majority of electron equivalents to reduce nitrate to N 2 ., (Copyright © 2017 Elsevier Ltd. All rights reserved.)

It remains unclear whether periphytic biofilms are beneficial to N cycling in paddy fields. Here, based on a national‐scale field investigation covering 220 rice fields in China, the N accumulation potential of periphytic biofilms was found to decrease from 8.8 ± 2.4 to 4.5 ± 0.7 g/kg and 3.1 ± 0.6 g/kg with increasing habitat latitude and longitude, respectively. The difference in abundant and rare subcommunities likely accounts for their geo‐difference in N accumulation potential. The N cycling pathways involved in periphytic biofilms inferred that soil N and N2 were two potential sources for N accumulation in periphytic biofilms. Meanwhile, some of the accumulated N may be lost via N2, N2O, NO, or NH3 outputs. Superficially, periphytic biofilms are double‐edged swords to N cycling by increasing soil N through biological N fixation but accelerating greenhouse gas emissions. Essentially, augmented periphytic biofilms increased change of TN (ΔTN) content in paddy soil from −231.9 to 31.9 mg/kg, indicating that periphytic biofilms overall benefit N content enhancement in paddy fields. This study highlights the contribution of periphytic biofilms to N cycling in rice fields, thus, drawing attention to their effect on rice production and environmental security. [ABSTRACT FROM AUTHOR]

Phototrophic biofilms are distributed widely at the sediment/soil-water interfaces (SWI) in paddy fields, where they immobilize phosphorus, thereby reducing its runoff loss. However, how soil carbon, nutrient availability and nutrient ratios drive the phototrophic biofilm community and its contribution to phosphorus cycling is largely unknown. A large scale field investigation in Chinese paddy fields reported here shows that soil organic carbon (SOC) and soil total nitrogen (STN) contents rather than soil total phosphorus (STP) triggered phosphorus immobilization of paddy biofilms, as they changed algal diversity and EPS production. High C: P and N: P ratios favored phosphorus immobilization in biofilm biomass via increasing the abundance of green algae. The C: N ratio on the other hand had only a weak effect on phosphorus immobilization, being counteracted by SOC or STN. Results from this study reveal how the in-situ interception of phosphorus in paddy fields is driven by soil carbon, nutrient availability and nutrient ratios and provide practical information on how to reduce runoff losses of phosphorus by regulating SOC and STN contents. Image 1 • Soil organic carbon, nitrogen, C/P and N/P are central in driving P immobilization. • Soil organic carbon and nitrogen counteract the effect of C/N on P immobilization. • Green algae and extracellular polymeric substances are vital in P immobilization. [ABSTRACT FROM AUTHOR]

Production of sulfide and methane due to anaerobic biological transformations in sewer pipes causes serious problems to sewer maintenance. For gravity sewers, enhancing ventilation is a practical method that reduces the production of both sulfide and methane. This study aimed to determine the effectiveness of a new method, Upstream Natural Pulsed Ventilation (UNPV), to control sulfide and methane production in gravity sewers. Two lab-scale reactors simulating the gravity sewer pipe with and without ventilation were set up to assess the effectiveness. The results show that compared with the gravity sewer pipe without ventilation, under the UNPV condition, the total sulfide concentration reduced by 39.08% and 58.74%, and the methane concentration reduced by 42.29% and 35.70% in the upstream and downstream sewer pipe, respectively. High-throughput sequencing analysis showed that the UNPV method could inhibit the proliferation of sulfate-reducing bacteria and stimulate the proliferation of sulfur-oxidizing bacteria within the whole sewer pipe. The composition of methanogenic archaea that are responsible for methane production was changed by ventilation. The increased oxidation-reduction potential and organic carbon transportation in wastewater under ventilation may be responsible for the microbial community changes. The findings of this study may provide new insight to reduce sulfide and methane production in gravity sewers. Unlabelled Image • A simple method to control harmful gas in sewer by ventilation was proposed. • Sulfide emissions were reduced by ≈60% during upstream ventilation. • Methane emissions were reduced by ≈40% during upstream ventilation. • The biofilm community under ventilation was elucidated. [ABSTRACT FROM AUTHOR]

• IAA less than 10 mg/L inhibits the growth of periphytic biofilm. • IAA more than 50 mg/L stimulates growth of periphytic biofilm. • The removal rate of IAA by periphytic biofilm is 95%–100%. • IAA more than 50 mg/L stimulates the capacity of periphytic biofilm to remove IAA. Due to its extensive application in agriculture as a germinating agent and growth promoter, indole acetic acid (IAA) is present in a variety of aquatic ecosystems. To explore the response of microbial aggregates to exogenous IAA in aquatic ecosystems, periphytic biofilm, a typical microbial aggregate, was exposed to IAA at different concentrations. Results reveal an unexpected concentration-dependent effect of IAA on periphytic biofilm. Concentrations of IAA less than 10 mg/L inhibit periphytic growth, but stimulate growth when the IAA concentration exceeds 50 mg/L. Periphytic biofilm adapts to different IAA concentrations by antioxidant enzyme activation, community structure optimization and carbon-metabolism pattern change, and promotes bioremediation of IAA contaminated water in the process. The removal rates of IAA reached up to 95%–100%. This study reveals the capacity of periphytic biofilm for IAA removal in practice. [ABSTRACT FROM AUTHOR]