1. Micro-Raman analysis and AFM imaging of Acidithiobacillus ferrooxidans biofilm grown on uranium ore.
- Author
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Pradhan N, Pradhan SK, Nayak BB, Mukherjee PS, Sukla LB, and Mishra BK
- Subjects
- Acidithiobacillus metabolism, Biopolymers chemistry, Biopolymers metabolism, India, Polysaccharides, Bacterial chemistry, Polysaccharides, Bacterial metabolism, Soil Microbiology, Acidithiobacillus chemistry, Biofilms, Image Processing, Computer-Assisted methods, Microscopy, Atomic Force methods, Spectrum Analysis, Raman methods, Uranium metabolism
- Abstract
Acidithiobacillus ferrooxidans biofilm grown on uranium ore substrate was analyzed by a micro-Raman spectrometer and an atomic force microscope (AFM). The bacterium employed for this study, A. ferrooxidans BM1, was isolated from a uranium mine (Jaduguda, India). Micro-Raman analysis revealed the different constituents of molecular fragments present in microbial cells and in secreted extracellular polymeric substances (EPSs). AFM images clearly revealed bacterial cells surrounded by EPS. From Raman spectral data, the composition of EPS from A. ferrooxidans BM1 appeared to be similar to that of EPS secreted in a different Pseudomonas bacterium.
- Published
- 2008
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