1. Cell wall composition and biofilm formation of azoles-susceptible and -resistant Candida glabrata strains.
- Author
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Vitali A, Vavala E, Marzano V, Leone C, Castagnola M, Iavarone F, and Angiolella L
- Subjects
- Adhesiveness, Azoles pharmacology, Candida glabrata drug effects, Candida glabrata isolation & purification, Candidiasis drug therapy, Candidiasis microbiology, Carbohydrate Metabolism drug effects, Cell Wall drug effects, Fungal Polysaccharides biosynthesis, Gene Expression Regulation, Fungal drug effects, Hydrophobic and Hydrophilic Interactions, Microbial Sensitivity Tests, Mutation, Proteomics methods, Species Specificity, Surface Properties, Two-Dimensional Difference Gel Electrophoresis, Antifungal Agents pharmacology, Biofilms drug effects, Candida glabrata physiology, Cell Wall metabolism, Drug Resistance, Multiple, Fungal, Fungal Proteins biosynthesis, Triazoles pharmacology
- Abstract
In the present study, three strains of Candida glabrata have been investigated to shed light on the mechanisms involved in azole resistance during adherence and biofilm formation. In particular, a clinical isolate, susceptible to azole-based drugs, DSY562 and two different resistant mutagenic strains deriving from DSY562, SFY114 and SFY115, have been analysed with different approaches for their cell wall composition and properties. A proteomic analysis revealed that the expression of six cell wall-related proteins and biofilm formation varied between the strains. The SFY114 and SFY115 strains resulted to be less hydrophobic than the susceptible parental counterpart DSY562, on the other hand they showed a higher amount in total cell wall polysaccharides fraction in the total cell wall. Accordingly to the results obtained from the hydrophobicity and adherence assays, in the resistant strain SFY115 the biofilm formation decreased compared to the parental strain DSY562. Finally, the total glucose amount in resistant SFY115 was about halved in comparison to other strains. Taken together all these data suggest that azole drugs may affect the cell wall composition of C. glabrata, in relation to the different pathogenic behaviours.
- Published
- 2017
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