1. Lysozyme regulates the extracellular polymer of activated sludge and promotes the formation of electroactive biofilm
- Author
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Xindi Jia, Xiaoliang Liu, Kaili Zhu, Xinxin Zheng, Zhiyuan Yang, Xue Yang, Yunhua Hou, and Qinzheng Yang
- Subjects
Sewage ,Extracellular Polymeric Substance Matrix ,Polymers ,Biofilms ,Bioengineering ,Muramidase ,General Medicine ,Biotechnology - Abstract
The formation of electroactive biofilm from activated sludge on electrode surface is a key step to construct a bio-electrochemical system, yet it is greatly limited by the poor affinity between the bacteria and the electrode interface. Herein, we report a new method to promote the formation of electroactive biofilm by regulating the extracellular polymeric substance (EPS) content in activated sludge with lysozyme. The investigation of the effect of lysozyme treatment on the content of extracellular polymers and the biofilm formation of electroactive bacteria suggests that lysozyme can improve the permeability of the positive bacterial cell membrane and thus increase the EPS content in the activated sludge. The characterizations of electrochemical activity, surface morphology and community structure of the anode biofilm indicate that increasing EPS content promotes the adhesion of the mixed bacteria in the activated sludge on the electrode and results in denser biofilms with better conductivities. The microbial fuel cell (MFC) inoculated with the sludge of high EPS content exhibits the power density up to 2.195 W/m
- Published
- 2022