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Ratiometric near-infrared fluorescent probes are powerful tools for studying the biological roles of hypochlorite in biological systems. However, due to the lack of suitable fluorescent molecular platforms, the development of near-infrared ratiometric hypochlorite probes remains a challenge. Here, we report the design and synthesis of a near-infrared fluorescent probe PTZCy , which is constructed using a phenothiazine-hemicyanine hybrid framework. The incorporation of phenothiazine into the hemicyanine scaffold enables the probe to exhibit a near-infrared emission maximum at approximately 820 nm, along with a rapid and highly selective ratiometric response to hypochlorite. Importantly, PTZCy can be used to image both endogenous and exogenous hypochlorite in RAW264.7 cells, as well as to perform ratiometric imaging of hypochlorite during the acute liver injury process in living mice. [Display omitted] • A phenothiazine-hemicyanine hybrid framework was rationally designed for detecting ClO−. • The probe exhibited an emission maximum at 820 nm, along with a rapid and highly selective ratiometric response to ClO−. • The probe was employed for ratiometric imaging of ClO− in living cells and in mice experiencing acute liver injury. [ABSTRACT FROM AUTHOR]

Hydrogen polysulfides (H 2 S n , n > 1) have attracted increasing attention in biological systems due to its redox signaling effect. To illustrate the process of the physiological and pathological roles played by H 2 S n , accurate detection is highly desired. In this work, we report a BODIPY-based fluorescent probe (BDP-PHS) for ratiometric H 2 S n sensing. BDP-PHS shows higher sensitivity and selectivity ratiometric response toward H 2 S n than various biological related species. Moreover, BDP-PHS has been successfully applied in imaging of H 2 S n in living cells. A new fluorescent probe, BDP-PHS , which exhibits a specific ratiometric response toward H 2 S n than various biological related species in both aqueous media and living cells was developed. Unlabelled Image • A new ratiometric fluorogenic probe (BDP-PHS) for detecting polysulfides was developed. • BDP-PHS shows specific polysulfides-induced emission shift from 574 to 593 nm. • BDP-PHS has been utilized to realize the in vivo ratiometric polysulfides imaging in living cells. [ABSTRACT FROM AUTHOR]