1. A fast and robust UHPLC-MRM-MS method to characterize and quantify grape skin tannins after chemical depolymerization
- Author
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Anna Vallverdú-Queralt, Emmanuelle Meudec, Nancy Terrier, Véronique Cheynier, Arnaud Verbaere, Jean-Claude Boulet, Maryline Roques, Lucie Pinasseau, Nicolas Sommerer, Sciences Pour l'Oenologie (SPO), Université Montpellier 1 (UM1)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), European Community (FP7-311775), European Project: 270704, Université Montpellier 1 (UM1)-Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie])-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université Montpellier 1 (UM1)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)
- Subjects
0106 biological sciences ,proanthocyanidine ,Phloroglucinol ,grapes ,tannins ,phloroglucinolysis ,quantitative UHPLC-QqQ-MS ,method validation ,metabolomics ,Pharmaceutical Science ,01 natural sciences ,Polymerization ,Analytical Chemistry ,chemistry.chemical_compound ,Tandem Mass Spectrometry ,Drug Discovery ,Tannin ,Vitis ,Chromatography, High Pressure Liquid ,chemistry.chemical_classification ,Vegetal Biology ,Molecular Structure ,food and beverages ,oenologie ,Proanthocyanidin ,Chemistry (miscellaneous) ,Molecular Medicine ,Mass spectrometry ,Vineyard ,Article ,Catalysis ,baie de raisin ,lcsh:QD241-441 ,Metabolomics ,lcsh:Organic chemistry ,[SDV.BV]Life Sciences [q-bio]/Vegetal Biology ,Proanthocyanidins ,Physical and Theoretical Chemistry ,Chromatography ,Depolymerization ,fungi ,010401 analytical chemistry ,Organic Chemistry ,Selected reaction monitoring ,High-Throughput Screening Assays ,0104 chemical sciences ,flavane ,chemistry ,peau ,analyse hplc uv ,Biologie végétale ,010606 plant biology & botany - Abstract
A rapid, sensitive, and selective analysis method using ultra high performance liquid chromatography coupled with triple-quadrupole mass spectrometry (UHPLC-QqQ-MS) has been developed for the characterization and quantification of grape skin flavan-3-ols after acid-catalysed depolymerization in the presence of phloroglucinol (phloroglucinolysis). The compound detection being based on specific MS transitions in Multiple Reaction Monitoring (MRM) mode, this fast gradient robust method allows analysis of constitutive units of grape skin proanthocyanidins, including some present in trace amounts, in a single injection, with a throughput of 6 samples per hour. This method was applied to a set of 214 grape skin samples from 107 different red and white grape cultivars grown under two conditions in the vineyard, irrigated or non-irrigated. The results of triplicate analyses confirmed the robustness of the method, which was thus proven to be suitable for high-throughput and large-scale metabolomics studies. Moreover, these preliminary results suggest that analysis of tannin composition is relevant to investigate the genetic bases of grape response to drought.
- Published
- 2016
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