137 results on '"Bao, Shi"'
Search Results
2. New cytochalasans from an endophytic Xylaria species associated with Costa Rican Palicourea elata (Rubiaceae)
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Monika Schmoll, Bao-Bao Shi, Markus Bacher, Johann Schinnerl, Lothar Brecker, Wolfgang Hinterdobler, Dieter Baurecht, Karin M. Valant-Vetschera, and Irmgard Krisai-Greilhuber
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endophytic fungi ,Rubiaceae ,biology ,Palicourea ,Organic Chemistry ,Xylaria ,Plant Science ,Fungus ,biology.organism_classification ,Biochemistry ,Plant use of endophytic fungi in defense ,Analytical Chemistry ,Germination ,Cytochalasans ,Botany ,Palicourea elata ,Xylariaceae ,Trichoderma reesei - Abstract
Four new leucine-derived cytochalasans, possessing a 5,6,5,8-ring (1) and a 5,6,11-ring core (2-4), were isolated from a cultivated endophytic fungus Xylaria sp. strain WH2D4 (Xylariaceae). This fungus was isolated from leaves of the neotropical tree species Palicourea elata (Sw.) Borhidi (Rubiaceae) collected in Costa Rica. The chemical structures were determined by employing IR, MS as well as 1D- and 2D-NMR experiments. The stereochemistry at C-15 of compound 4 was determined by quantum calculations. The isolated compounds did not affect germination and growth of Trichoderma reesei and the opportunistic human fungal pathogen T. longibrachiatum.
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- 2021
3. α‐synuclein suppresses microglial autophagy and promotes neurodegeneration in a mouse model of Parkinson’s disease
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Zheng-Hong Qin, Xiao-ou Hou, Xiao-Jun Zhang, Ya-Ping Yang, Chun-Feng Liu, Ya-Ting Ma, Bao-Shi Yuan, Yi Fan, Chong-Shuang Pei, Li-Fang Hu, and Hai-Yue Tu
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autophagy ,Aging ,Parkinson's disease ,animal diseases ,p38 mitogen-activated protein kinases ,ATG5 ,microglia ,Biology ,neuroinflammation ,Mice ,medicine ,Animals ,heterocyclic compounds ,Protein kinase B ,Neuroinflammation ,PI3K/AKT/mTOR pathway ,α‐synuclein ,Microglia ,Neurodegeneration ,Autophagy ,Parkinson Disease ,Cell Biology ,medicine.disease ,Original Papers ,nervous system diseases ,Cell biology ,Disease Models, Animal ,medicine.anatomical_structure ,nervous system ,Neuroinflammatory Diseases ,alpha-Synuclein ,Original Article - Abstract
The cell‐to‐cell transfer of α‐synuclein (α‐Syn) greatly contributes to Parkinson's disease (PD) pathogenesis and underlies the spread of α‐Syn pathology. During this process, extracellular α‐Syn can activate microglia and neuroinflammation, which plays an important role in PD. However, the effect of extracellular α‐Syn on microglia autophagy is poorly understood. In the present study, we reported that extracellular α‐Syn inhibited the autophagy initiation, as indicated by LC3‐II reduction and p62 protein elevation in BV2 and cultured primary microglia. The in vitro findings were verified in microglia‐enriched population isolated from α‐Syn‐overexpressing mice induced by adeno‐associated virus (AAV2/9)‐encoded wildtype human α‐Syn injection into the substantia nigra (SN). Mechanistically, α‐Syn led to microglial autophagic impairment through activating toll‐like receptor 4 (Tlr4) and its downstream p38 and Akt‐mTOR signaling because Tlr4 knockout and inhibition of p38, Akt as well as mTOR prevented α‐Syn‐induced autophagy inhibition. Moreover, inhibition of Akt reversed the mTOR activation but failed to affect p38 phosphorylation triggered by α‐Syn. Functionally, the in vivo evidence showed that lysozyme 2 Cre (Lyz2 cre)‐mediated depletion of autophagy‐related gene 5 (Atg5) in microglia aggravated the neuroinflammation and dopaminergic neuron losses in the SN and exacerbated the locomotor deficit in α‐Syn‐overexpressing mice. Taken together, the results suggest that extracellular α‐Syn, via Tlr4‐dependent p38 and Akt‐mTOR signaling cascades, disrupts microglial autophagy activity which synergistically contributes to neuroinflammation and PD development., Autophagy‐dependent and independent machinery synergistically contribute to hα‐Syn‐caused neuroinflammation in PD. The basal autophagy activity restricts microglia inflammation. Extracellular hα‐Syn interacts with and activates Tlr4, resulting in inflammatory responses, as well as autophagy suppression in microglia via Tlr4‐dependent p38 and Akt/mTOR signaling cascades. This impairs the inhibitory effect of autophagy on inflammation, and thus aggravating hα‐Syn‐induced inflammatory responses.
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- 2021
4. AZ32 Reverses ABCG2-Mediated Multidrug Resistance in Colorectal Cancer
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Zhe-Sheng Chen, Zhi Shi, Zi-Hao Xing, Zheng-Jie He, Yan-Chi Li, Peng-Wei Zhang, Ze-Zhong Yu, Xue-Mei Mo, Yu Chen, Xiao-Bao Shi, Sheng-Te Wang, Meiwan Chen, Wei-Jing Liu, and Kun Liu
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0301 basic medicine ,Cancer Research ,animal structures ,Abcg2 ,Colorectal cancer ,ABCG2 ,colorectal cancer ,Drug resistance ,Malignancy ,AZ32 ,03 medical and health sciences ,0302 clinical medicine ,multidrug resistance ,Medicine ,Doxorubicin ,RC254-282 ,Mitoxantrone ,biology ,business.industry ,Kinase ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Brief Research Report ,medicine.disease ,Multiple drug resistance ,030104 developmental biology ,Oncology ,CRISPR ,030220 oncology & carcinogenesis ,embryonic structures ,biology.protein ,Cancer research ,sense organs ,business ,medicine.drug - Abstract
Colorectal cancer is a common malignancy with the third highest incidence and second highest mortality rate among all cancers in the world. Chemotherapy resistance in colorectal cancer is an essential factor leading to the high mortality rate. The ATP-binding cassette (ABC) superfamily G member 2 (ABCG2) confers multidrug resistance (MDR) to a range of chemotherapeutic agents by decreasing their intracellular content. The development of novel ABCG2 inhibitors has emerged as a tractable strategy to circumvent drug resistance. In this study, an ABCG2-knockout colorectal cancer cell line was established to assist inhibitor screening. Additionally, we found that ataxia-telangiectasia mutated (ATM) kinase inhibitor AZ32 could sensitize ABCG2-overexpressing colorectal cancer cells to ABCG2 substrate chemotherapeutic drugs mitoxantrone and doxorubicin by retaining them inside cells. Western blot assay showed that AZ32 did not alter the expression of ABCG2. Moreover, molecule docking analysis predicted that AZ32 stably located in the transmembrane domain of ABCG2. In conclusion, our result demonstrated that AZ32 could potently reverse ABCG2-mediated MDR in colorectal cancer.
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- 2021
5. Integrated lncRNA and mRNA Transcriptome Analyses in the Ovary of Cynoglossus semilaevis Reveal Genes and Pathways Potentially Involved in Reproduction
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Yani Dong, Likang Lyu, Daiqiang Zhang, Jing Li, Haishen Wen, and Bao Shi
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Competing endogenous RNA ,Wnt signaling pathway ,Cynoglossus semilaevis ,QH426-470 ,Biology ,Oocyte ,integrated analysis ,Cell biology ,reproduction ,Transcriptome ,medicine.anatomical_structure ,Gene expression ,Genetics ,medicine ,Molecular Medicine ,ovary ,dual-fluorescence in situ hybridization ,KEGG ,Signal transduction ,transcriptome ,Gene ,Genetics (clinical) - Abstract
Long non-coding RNAs (lncRNAs) have been reported to be involved in multiple biological processes. However, the roles of lncRNAs in the reproduction of half-smooth tongue sole (Cynoglossus semilaevis) are unclear, especially in the molecular regulatory mechanism driving ovarian development and ovulation. Thus, to explore the mRNA and lncRNA mechanisms regulating reproduction, we collected tongue sole ovaries in three stages for RNA sequencing. In stage IV vs. V, we identified 312 differentially expressed (DE) mRNAs and 58 DE lncRNAs. In stage V vs. VI, we identified 1,059 DE mRNAs and 187 DE lncRNAs. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analyses showed that DE mRNAs were enriched in ECM-receptor interaction, oocyte meiosis and steroid hormone biosynthesis pathways. Furthermore, we carried out gene set enrichment analysis (GSEA) to identify potential reproduction related-pathways additionally, such as fatty metabolism and retinol metabolism. Based on enrichment analysis, DE mRNAs with a potential role in reproduction were selected and classified into six categories, including signal transduction, cell growth and death, immune response, metabolism, transport and catabolism, and cell junction. The interactions of DE lncRNAs and mRNAs were predicted according to antisense, cis-, and trans-regulatory mechanisms. We constructed a competing endogenous RNA (ceRNA) network. Several lncRNAs were predicted to regulate genes related to reproduction including cyp17a1, cyp19a1, mmp14, pgr, and hsd17b1. The functional enrichment analysis of these target genes of lncRNAs revealed that they were involved in several signaling pathways, such as the TGF-beta, Wnt signaling, and MAPK signaling pathways and reproduction related-pathways such as the progesterone-mediated oocyte maturation, oocyte meiosis, and GnRH signaling pathway. RT-qPCR analysis showed that two lncRNAs (XR_522278.2 and XR_522171.2) were mainly expressed in the ovary. Dual-fluorescence in situ hybridization experiments showed that both XR_522278.2 and XR_522171.2 colocalized with their target genes cyp17a1 and cyp19a1, respectively, in the follicular cell layer. The results further demonstrated that lncRNAs might be involved in the biological processes by modulating gene expression. Taken together, this study provides lncRNA profiles in the ovary of tongue sole and further insight into the role of lncRNA involvement in regulating reproduction in tongue sole.
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- 2021
6. Microbiota characteristics in Sebastes schlegelii intestine in early life stages
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Yongjiang Xu, Bao Shi, Xuezhou Liu, Bin Wang, and Yan Jiang
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biology ,Glaciecola ,ved/biology ,Firmicutes ,ved/biology.organism_classification_rank.species ,Zoology ,Oceanography ,biology.organism_classification ,digestive system ,Pseudoalteromonas ,Lactobacillus ,Aliivibrio ,Sebastes schlegelii ,Proteobacteria ,Water Science and Technology ,Black rockfish - Abstract
The structure of intestinal microbiota of black rockfish Sebastes schlegelii in five early development stages were determined in high throughput sequencing with Illumina MiSeq PE300 system. The relationship between intestinal microbial community and the environmental (including culture water and feed) microbiota and the abundance variation trends of core microbiota were investigated, based on which the source of some core microbiota was analyzed in this study. The results show that Proteobacteria and Firmicutes are the most dominant phyla in guts. At the genus level, there are obvious differences between the artificial breeding fish and wild adults in the intestinal microflora structure. The compositions of dominant genera are similar, although the structure of intestinal microbiota gradually changes with the growth of larvae and juveniles. The core microbiota including Bacillus, Acinetobacter, Pseudomonas, Lactobacillus, Lactococcus, Glaciecola, Vibrio, Pseudoalteromonas, Acidovorax, and Aliivibrio were determined in the analysis of dominant and shared species. Compared with the water, the effect of feed microbiota on the structure of the gut microbial community is more obvious. Moreover, the trends of Bacillus, Acinetobacter, Pseudomonas, Lactobacillus, Lactococcus, and Glaciecola were opposite to Vibrio and Pseudoalteromonas in the gut. The correlation analysis suggested that Acidovorax, Glaciecola, Pseudomonas, Lactobacillus, and Acinetobacter might transited from mainly the parents and/or came from the fertilization process. The relative results may provide a theoretical reference for selecting the native probiotics, and supply the basic data for artificially regulating the intestinal microbiota with probiotic during early developmental stage of black rockfish.
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- 2019
7. Four Yellow Monoterpenoid Quinoline Alkaloids from the Stem of Tabernaemontana bovina
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Bao-Bao Shi, Yi Wang, Yang Yu, Mei-Fen Bao, Jing Wu, Xiang-Hai Cai, and Johann Schinnerl
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Lipopolysaccharides ,Models, Molecular ,Tabernaemontana ,Stereochemistry ,Nitric oxide biosynthesis ,Crystallography, X-Ray ,Nitric Oxide ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Mice ,chemistry.chemical_compound ,Alkaloids ,Animals ,Physical and Theoretical Chemistry ,Molecular Structure ,biology ,010405 organic chemistry ,Chemistry ,Macrophages ,Alkaloid ,Organic Chemistry ,Quinoline ,biology.organism_classification ,0104 chemical sciences ,RAW 264.7 Cells ,Quinolines - Abstract
Four unprecedented yellow alkaloids named as taberbovines A-D (1-4) were isolated from stems of Tabernaemontana bovina Lour. Their structures were elucidated by the comprehensive spectroscopic data, computational chemistry, and X-ray crystal diffraction. Alkaloids 1-4 possessed a unique 6/6/5/6/5 ring system and were assigned to monoterpenoid quinolines. Biosynthetically, the isolated alkaloids may be derived from Aspidosperma-type alkaloid by oxidation and rearrangements. Taberbovines A-D exhibited the good inhibitory activities of formation NO in LPS induced RAW264.7 macrophages.
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- 2019
8. Developmental expression of LPXRFa, kisspeptin, and their receptor mRNAs in the half-smooth tongue sole Cynoglossus semilaevis
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Yongjiang Xu, Yan Jiang, Yaxing Zhang, Bao Shi, Aijun Cui, Bin Wang, and Xuezhou Liu
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0106 biological sciences ,biology ,010604 marine biology & hydrobiology ,media_common.quotation_subject ,Embryogenesis ,04 agricultural and veterinary sciences ,Aquatic Science ,biology.organism_classification ,Ligand (biochemistry) ,01 natural sciences ,Cell biology ,Flatfish ,Kisspeptin ,040102 fisheries ,0401 agriculture, forestry, and fisheries ,Reproduction ,Receptor ,Smooth tongue ,Hormone ,media_common - Abstract
Both gonadotropin-inhibitory hormone (GnIH) and kisspeptin play a critical role in the control of reproduction in vertebrates. However, the spatial–temporal expression patterns and possible roles of these two peptides during development are poorly understood. To this end, we evaluated dynamic expression profiles of lpxrfa, the piscine ortholog of gnih, kisspeptin (kiss2) and their receptor mRNAs during embryogenesis and early larval development in a flatfish, Cynoglossus semilaevis. Quantitative RT-PCR analysis indicated that both ligand (lpxrfa and kiss2) and receptor (lpxrfa-r and kiss2r) mRNAs were detected in unfertilized eggs and during embryogenesis, but with different expression profiles. In addition, lpxrfa-r, kiss2 and kiss2r transcripts increased significantly at 6 days post-hatching (dph), while lpxrfa mRNAs did not vary significantly during all larval development. Taken together, these results of the present study suggest that both LPXRFa and kisspeptin systems could be functional and play differential roles during embryogenesis and early larval development in tongue sole.
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- 2019
9. Structural characteristics and succession of intestinal microbiota for Paralichthys olivaceus during the early life stage
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Bao Shi, Yongjiang Xu, Bin Wang, Yan Jiang, Zengxin Liu, and Xuezhou Liu
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0106 biological sciences ,0301 basic medicine ,Paralichthys ,biology ,Zoology ,Ecological succession ,Aquatic Science ,biology.organism_classification ,01 natural sciences ,Early life ,03 medical and health sciences ,030104 developmental biology ,Stage (stratigraphy) ,010608 biotechnology - Published
- 2018
10. Leptin and leptin receptor genes in tongue sole (Cynoglossus semilaevis): Molecular cloning, tissue distribution and differential regulation of these genes by sex steroids
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Bin Wang, Kangli Ren, Bao Shi, Xuesong Song, Xuezhou Liu, Quan Liu, Yaxing Zhang, and Yongjiang Xu
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Fish Proteins ,Leptin ,0301 basic medicine ,medicine.medical_specialty ,DNA, Complementary ,Physiology ,Growth hormone receptor ,Biology ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Energy homeostasis ,03 medical and health sciences ,Complementary DNA ,Internal medicine ,Gene expression ,medicine ,Animals ,Tissue Distribution ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Molecular Biology ,Gene ,Phylogeny ,Messenger RNA ,Leptin receptor ,Base Sequence ,Sequence Homology, Amino Acid ,Reverse Transcriptase Polymerase Chain Reaction ,030104 developmental biology ,Endocrinology ,Flatfishes ,Receptors, Leptin ,Female - Abstract
Leptin (Lep) is a key factor for the regulation of food intake and energy homeostasis in mammals. To date, a number of studies have provided evidence for the existence of multiple leptin genes in teleosts, but not much information is available in fish regarding the regulation of leptin genes by sex steriods. As a first step, two leptin genes (lepa and lepb) and a leptin receptor (lepr) gene were cloned from the half-smooth tongue sole (Cynoglossus semilaevis), a representative species of the order Pleuronectiformes. The full-length cDNAs of tongue sole lepa and lepb were 1265 bp and 1157 bp in length, encoding for proteins of 160 aa and 158 aa, respectively. The three-dimensional structures modeling of tongue sole LepA and LepB showed strong conservation of tertiary structure with other vertebrates. The full-length cDNA of tongue sole lepr was 4576 bp, encoding a protein of 1133 aa which contained all functionally important domains conserved among vertebrate LepRs. Tissue distribution analysis showed that tongue sole lepa mRNA was highly detectable in the ovary and brain, while lepb mRNA was ubiquitously expressed in various tissues. Notably, the tongue sole lepr mRNA was most abundant in the ovary. Using a primary hepatocyte culture system, we evaluated the effects of sex steroids on lep/lepr gene expression. Both 17β-estradiol (E2) and testosterone (T) inhibited hepatic lepa and lepr mRNAs without affecting lepb mRNA levels. In addition, T also suppressed growth hormone receptor 1 (ghr1), ghr2, and insulin-like growth factor 2 (igf-2) mRNA levels, and stimulated expression of igf-1 gene. On the other hand, none of these four genes were altered by E2. To the best of our knowledge, this is the first description of a direct and differential regulation of lep/lepr gene expression by sex steroids at the hepatocyte level of a flatfish, supporting that individual leptin peptide may possess different biological roles in teleosts.
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- 2018
11. Discovery of Selenocysteine as a Potential Nanomedicine Promotes Cartilage Regeneration With Enhanced Immune Response by Text Mining and Biomedical Databases
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Bao-Shi Fan, Yi-Fan Song, Shi-Tang Song, You-Rong Chen, Jing Ye, Jiying Zhang, Meng Yang, Jia-Kuo Yu, Bing-Bing Xu, Sun Zewen, Fu-Zhen Yuan, and Xin Yan
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0301 basic medicine ,Microarray ,Osteoarthritis ,text mining ,Biology ,computer.software_genre ,Regenerative medicine ,immune response ,clinical translation ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Pharmacology (medical) ,KEGG ,cartilage ,Original Research ,Pharmacology ,Cell chemotaxis ,biomaterial drug ,Database ,TOLLIP ,Cartilage ,Regeneration (biology) ,lcsh:RM1-950 ,medicine.disease ,nanomedicine ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,computer - Abstract
Background Unlike bone tissue, little progress has been made regarding cartilage regeneration, and many challenges remain. Furthermore, the key roles of cartilage lesion caused by traumas, focal lesion, or articular overstress remain unclear. Traumatic injuries to the meniscus as well as its degeneration are important risk factors for long-term joint dysfunction, degenerative joint lesions, and knee osteoarthritis (OA) a chronic joint disease characterized by degeneration of articular cartilage and hyperosteogeny. Nearly 50% of the individuals with meniscus injuries develop OA over time. Due to the limited inherent self-repair capacity of cartilage lesion, the Biomaterial drug-nanomedicine is considered to be a promising alternative. Therefore, it is important to elucidate the gene potential regeneration mechanisms and discover novel precise medication, which are identified through this study to investigate their function and role in pathogenesis. Methods We downloaded the mRNA microarray statistics GSE117999, involving paired cartilage lesion tissue samples from 12 OA patients and 12 patients from a control group. First, we analyzed these statistics to recognize the differentially expressed genes (DEGs). We then exposed the gene ontology (GO) annotation and the Kyoto Encyclopaedia of Genes and Genomes (KEGG) pathway enrichment analyses for these DEGs. Protein-protein interaction (PPI) networks were then constructed, from which we attained eight significant genes after a functional interaction analysis. Finally, we identified a potential nanomedicine attained from this assay set, using a wide range of inhibitor information archived in the Search Tool for the Retrieval of Interacting Genes (STRING) database. Results Sixty-six DEGs were identified with our standards for meaning (adjusted P-value < 0.01, |log2 - FC| ≥1.2). Furthermore, we identified eight hub genes and one potential nanomedicine - Selenocysteine based on these integrative data. Conclusion We identified eight hub genes that could work as prospective biomarkers for the diagnostic and biomaterial drug treatment of cartilage lesion, involving the novel genes CAMP, DEFA3, TOLLIP, HLA-DQA2, SLC38A6, SLC3A1, FAM20A, and ANO8. Meanwhile, these genes were mainly associated with immune response, immune mediator induction, and cell chemotaxis. Significant support is provided for obtaining a series of novel gene targets, and we identify potential mechanisms for cartilage regeneration and final nanomedicine immunotherapy in regenerative medicine.
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- 2020
12. Potential roles of telomeres and telomerase in neurodegenerative diseases
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Jun Liang, Yaru Liu, Xinhua Liu, Quan Xia, Qingrong Xia, Chenchen Yang, Bao-Shi Wang, and Jiequan Wang
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Senescence ,DNA Replication ,Telomerase ,Aging ,Cell division ,Cell Survival ,02 engineering and technology ,Disease ,Degeneration (medical) ,Biology ,Biochemistry ,Neuroprotection ,Pathogenesis ,03 medical and health sciences ,Structural Biology ,Animals ,Humans ,Molecular Biology ,030304 developmental biology ,Cell Proliferation ,Neurons ,0303 health sciences ,Neurodegenerative Diseases ,General Medicine ,Telomere ,021001 nanoscience & nanotechnology ,Cell biology ,0210 nano-technology - Abstract
Telomeres, protective DNA-protein complexes at the end of eukaryotic linear chromosomes, play pivotal roles in the maintenance of genomic stability during cell division. When telomeres are severely shortened, cells stop dividing and die, consequently leading to tissues degeneration. Concretely, replicative senescence and genomic damage are generally accompanied with telomere shortening, which may be a potential contributor in the pathogenesis of neurological disorders. Regardless of occasional negative findings, accelerated telomere erosion is routinely found in neurodegenerative diseases and has been believed to be positively correlated with the severity of neurodegenerative diseases. As considerable knowledge of telomeres and telomerase continues to accumulate, telomerase is increasingly being recognized as a promising therapeutic target for neurodegenerative disease. Until now, strong evidence has accumulated that activated telomerase is responsible for telomere elongation that may be sufficient to prevent "mother cells" from replicative aging, and besides, telomerase activators exhibit remarkable neuroprotective effects through the prolongation of telomere length and the promotion of neuronal survival as well as proliferation. Therefore, a consensus is emerging that the activation of telomerase, promoted by peptides, natural herbal extracts, small molecules compounds and others, represents a novel promising treatment strategy for neurodegenerative diseases.
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- 2020
13. Genome-Wide Identification and Characterization of UTR-Introns of Citrus sinensis
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Binghua Wu, Jiapeng Liu, Na Tian, Fan Liu, Chunzhen Cheng, Xiao-bao Shi, Guangyan Zhong, Jing-Ru Che, Jialan Chen, Raphael Anue Mensah, Junwei Wu, and Ye Guo
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0106 biological sciences ,0301 basic medicine ,Untranslated region ,Biology ,Regulatory Sequences, Nucleic Acid ,Cis- elements ,01 natural sciences ,Genome ,nucleotide distribution ,Catalysis ,Article ,Inorganic Chemistry ,lcsh:Chemistry ,03 medical and health sciences ,Open Reading Frames ,UTR intron ,Untranslated Regions ,Gene expression ,Physical and Theoretical Chemistry ,Molecular Biology ,Gene ,3' Untranslated Regions ,lcsh:QH301-705.5 ,Spectroscopy ,Regulation of gene expression ,Genetics ,Organic Chemistry ,Alternative splicing ,Intron ,Chromosome Mapping ,General Medicine ,Genomics ,Introns ,Computer Science Applications ,Alternative Splicing ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,RNA splicing ,gene expression ,RNA Splice Sites ,5' Untranslated Regions ,Genome, Plant ,010606 plant biology & botany ,Citrus sinensis ,Genome-Wide Association Study - Abstract
Introns exist not only in coding sequences (CDSs) but also in untranslated regions (UTRs) of a gene. Recent studies in animals and model plants such as Arabidopsis have revealed that the UTR-introns (UIs) are widely presented in most genomes and involved in regulation of gene expression or RNA stability. In the present study, we identified introns at both 5&prime, UTRs (5UIs) and 3&prime, UTRs (3UIs) of sweet orange genes, investigated their size and nucleotide distribution characteristics, and explored the distribution of cis-elements in the UI sequences. Functional category of genes with predicted UIs were further analyzed using GO, KEGG, and PageMan enrichment. In addition, the organ-dependent splicing and abundance of selected UI-containing genes in root, leaf, and stem were experimentally determined. Totally, we identified 825 UI- and 570 3UI-containing transcripts, corresponding to 617 and 469 genes, respectively. Among them, 74 genes contain both 5UI and 3UI. Nucleotide distribution analysis showed that 5UI distribution is biased at both ends of 5&prime, UTR whiles 3UI distribution is biased close to the start site of 3&prime, UTR. Cis- elements analysis revealed that 5UI and 3UI sequences were rich of promoter-enhancing related elements, indicating that they might function in regulating the expression through them. Function enrichment analysis revealed that genes containing 5UI are significantly enriched in the RNA transport pathway. While, genes containing 3UI are significantly enriched in splicesome. Notably, many pentatricopeptide repeat-containing protein genes and the disease resistance genes were identified to be 3UI-containing. RT-PCR result confirmed the existence of UIs in the eight selected gene transcripts whereas alternative splicing events were found in some of them. Meanwhile, qRT-PCR result showed that UIs were differentially expressed among organs, and significant correlation was found between some genes and their UIs, for example: The expression of VPS28 and its 3UI was significantly negative correlated. This is the first report about the UIs in sweet orange from genome-wide level, which could provide evidence for further understanding of the role of UIs in gene expression regulation.
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- 2020
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14. The Higher Inherent Therapeutic Potential of Biomaterial-Based hDPSCs and hEnSCs for Pancreas Diseases
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Bingbing Xu, Fu-Zhen Yuan, Lin Lin, Jing Ye, Bao-Shi Fan, Ji-Ying Zhang, Meng Yang, Dong Jiang, Wen-Bo Jiang, Xing Wang, and Jia-Kuo Yu
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0301 basic medicine ,Histology ,hADSCs ,lcsh:Biotechnology ,Biomedical Engineering ,Adipose tissue ,Bioengineering ,02 engineering and technology ,hDPSCs ,03 medical and health sciences ,In vivo ,Laminin ,lcsh:TP248.13-248.65 ,Dental pulp stem cells ,hEnSCs ,Medicine ,Original Research ,Matrigel ,biology ,business.industry ,Mesenchymal stem cell ,biomaterial ,Bioengineering and Biotechnology ,021001 nanoscience & nanotechnology ,Transplantation ,030104 developmental biology ,biology.protein ,Cancer research ,orthotopic transplantation ,Stem cell ,0210 nano-technology ,business ,Biotechnology - Abstract
Human endometrial stem cells (hEnSCs), dental pulp stem cells (hDPSCs) and adipose tissue-derived stem cells (hADSCs) are considered to be the promising candidates for the treatment of pancreas diseases. The prognosis is better with in situ injection of mesenchymal stem cells (MSCs) to the damaged pancreas compared with intravenous injection. However, the clinical application of these cells are limited, due to poor engraftment of transplanted cells after delivery. On the other hand, understanding the role of the biomaterials in cell therapy is essential to promote the therapeutic effects of MSCs. Matrigel, a basement membrane matrix biomaterial, is rich in laminin and collagen IV. The aim of this study is to investigate the difference of biological characteristics of hEnSCs, hDPSCs and hADSCs in vitro and their survival situation with Matrigel post intrapancreatic transplantation in vivo. Our findings showed, firstly, there was no significant difference in morphology and immunophenotype of these MSCs. Secondly, the biological properties, including cell proliferation, the ability of adipogenic and osteogenic differentiation and the mRNA expression levels of pancreas development-related genes, have been showed distinct difference among these MSCs. Thirdly, Matrigel can improve the survival of MSCs in vivo, especially for Matrigel-based hDPSCs and Matrigel-based hEnSCs in pancreas parenchyma of SD rats. These results suggest that hDPSCs and hEnSCs are with the greater inherent therapeutic potential for pancreas diseases compared with hADSCs.
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- 2020
15. Molecular characterization and expression profiles of insulin-like growth factors in yellowtail kingfish (Seriola lalandi) during embryonic development
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Yaxing Zhang, Bao Shi, Quan Liu, Xuezhou Liu, Yongjiang Xu, Bin Wang, and Yongshan Liu
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Signal peptide ,endocrine system ,Embryo, Nonmammalian ,animal structures ,Physiology ,medicine.medical_treatment ,Embryonic Development ,Aquatic Science ,Biology ,Biochemistry ,03 medical and health sciences ,Insulin-like growth factor ,food ,Somatomedins ,Complementary DNA ,Gene expression ,medicine ,Animals ,Amino Acid Sequence ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Seriola lalandi ,Base Sequence ,Embryogenesis ,Fishes ,Gene Expression Regulation, Developmental ,04 agricultural and veterinary sciences ,General Medicine ,Molecular biology ,food.food ,Amino acid ,Open reading frame ,chemistry ,040102 fisheries ,0401 agriculture, forestry, and fisheries - Abstract
In this study, to understand the role of the insulin-like growth factor (IGF) system in the regulation of early development in yellowtail kingfish (YTK, Seriola lalandi), an economically important marine fish species with a high potential for aquaculture, we first cloned the full-length cDNAs for igf1 and igf2 from the liver. YTK igf1 cDNA was 1946 base pairs (bp) in length with an open reading frame (ORF) of 558 bp encoding preproIGF1 of 185 amino acids (aa). The preproIGF1 consisted of 44 aa for the signal peptide, 68 aa for the mature peptide comprising B, C, A, and D domains, and 73 aa for the E domain. YTK igf2 cDNA had an ORF of 648 bp that encoded a total of 215 aa spanning the signal peptide (47 aa), the mature peptide (70 aa), and the E domain (98 aa). At the protein level, both YTK IGF1 and IGF2 exhibited high sequence identities with their corresponding fish counterparts, respectively. Subsequently, quantitative RT-PCR analysis indicated that the highest level of igf1 mRNA expression was recorded in the gonad and liver, while the igf2 mRNA expression was most abundant in the gill and liver. In addition, both igf1 and igf2 were detected in all stages of embryonic development and exhibited different gene expression patterns, supporting that IGF1 and IGF2 could be functional and play important roles during YTK embryogenesis. Overall, this initial study of IGF1 and IGF2 provides an insight into the endocrine mechanism involved in the early development of yellowtail kingfish.
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- 2018
16. Three New Indole Alkaloids from Tabernaemontana divaricata
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Xiang-Hai Cai, Jing Wu, Mei-Fen Bao, Yan Deng, and Bao-Bao Shi
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3α-hydroxymethyl-ibogamine ,Monoterpene ,Tabernaemontana divaricata ,Plant Science ,Toxicology ,01 natural sciences ,Biochemistry ,Analytical Chemistry ,HeLa ,3α-acetatemethoxyl-ibogamine ,lcsh:Botany ,medicine ,Bioorganic chemistry ,16α-hydroxyl-ibogamine ,heterocyclic compounds ,Cytotoxicity ,Pharmacology ,Indole test ,Traditional medicine ,biology ,Apocynaceae ,010405 organic chemistry ,Chemistry ,organic chemicals ,Organic Chemistry ,Monoterpene indole alkaloids ,Cancer ,medicine.disease ,biology.organism_classification ,lcsh:QK1-989 ,0104 chemical sciences ,010404 medicinal & biomolecular chemistry ,Original Article ,Food Science - Abstract
Three new monoterpene indole alkaloids, 3α-hydroxymethyl-ibogamine (1), 3α-acetatemethoxyl-ibogamine (2), 16α-hydroxyl-ibogamine (3) together with six known alkaloids were isolated from the branches and leaves of Tabernaemontana divaricata (Apocynaceae). The structures of these alkaloids were determined by spectroscopic analyses. All isolated compounds showed no significant cytotoxicity against SGC-7901 gastric cancer, HeLa, and A-549 lung cancer cell lines (IC50 > 20 µM). Graphical Abstract Electronic supplementary material The online version of this article (10.1007/s13659-018-0166-x) contains supplementary material, which is available to authorized users.
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- 2018
17. Recombinant expression and comparative bioactivity of tongue sole insulin-like growth factor (IGF)-1 and IGF-2 in Pichia pastoris
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Bao Shi, Yongjiang Xu, Xuezhou Liu, Bin Wang, and Li Bin
- Subjects
0301 basic medicine ,Recombinant expression ,medicine.medical_treatment ,Aquatic Science ,Biology ,biology.organism_classification ,Pichia pastoris ,03 medical and health sciences ,Insulin-like growth factor ,030104 developmental biology ,medicine.anatomical_structure ,Biochemistry ,Tongue ,Cynoglossus semilaevis ,medicine - Published
- 2018
18. Association between Helicobacter pylori infection and gallbladder diseases: A retrospective study
- Author
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Mei-Yan Xu, Jian Yin, Jia-Hui Ma, Qing-Bin Lu, Lan Liu, and Bao-Shi Yuan
- Subjects
medicine.medical_specialty ,food.ingredient ,Hepatology ,biology ,business.industry ,Gallbladder disease ,Gastroenterology ,Retrospective cohort study ,Odds ratio ,Gallstones ,Helicobacter pylori ,biology.organism_classification ,medicine.disease ,Confidence interval ,03 medical and health sciences ,0302 clinical medicine ,food ,030220 oncology & carcinogenesis ,Internal medicine ,medicine ,Cholecystitis ,030211 gastroenterology & hepatology ,business ,Polypus - Abstract
Background and aim The association between Helicobacter pylori (H. pylori) and gallbladder diseases is still unclear and is controversial. We conducted a retrospective study to clarify the prevalence of gallbladder diseases and factors related to gallbladder diseases and relationships between H. pylori infection, gallstones, cholecystitis, and cholecystic polypus. Methods The retrospective study was performed at the Aerospace Center Hospital in Beijing. The subjects in this study were a healthy population who underwent health examinations at the hospital between 2012 and 2015. The logistic regression models were used to explore the relationships between H. pylori infection and gallbladder diseases. Results There were 7803 (43.4%) subjects with H. pylori infection, 995 (5.5%) with gallstones, 219 (1.2%) with cholecystitis, and 1003 (5.6%) with cholecystic polypus amongst 17 971 subjects, respectively. In subjects aged 45 years or less, the prevalence of gallstones in the H. pylori (+) group was lower than that in the H. pylori (-) group (odds ratio = 0.653; 95% confidence interval: 0.468-0.911; P = 0.012). The prevalence of cholecystic polypus in the H. pylori (+) group was significantly higher than that in the H. pylori (-) group (odds ratio = 1.160; 95% confidence interval: 1.012-1.328; P = 0.033). Conclusions Helicobacter pylori infection was related with cholecystic polypus and gallstones in a Chinese population.
- Published
- 2018
19. Molecular characterization and expression profiles of LPXRFa at the brain-pituitary-gonad axis of half-smooth tongue sole ( Cynoglossus semilaevis ) during ovarian maturation
- Author
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Bao Shi, Quan Liu, Yongjiang Xu, Bin Wang, and Xuezhou Liu
- Subjects
Fish Proteins ,0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Gonad ,Physiology ,medicine.drug_class ,Ovary ,Biology ,Biochemistry ,Preprohormone ,03 medical and health sciences ,Basal (phylogenetics) ,Internal medicine ,medicine ,Animals ,Cloning, Molecular ,Molecular Biology ,Messenger RNA ,Hypothalamic Hormones ,Fishes ,Open reading frame ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,Female ,Gonadotropin ,Hormone - Abstract
Gonadotropin-inhibitory hormone (GnIH) has been characterized by its ability to inhibit either basal or gonadotropin-releasing hormone (GnRH)-induced gonadotropin synthesis and release in birds and mammals. However, the physiological role of GnIH on the reproductive axis in fish remains inconclusive, with most studies focusing on the orders Cypriniformes and Perciformes. To gain insight into the role of GnIH in the regulation of reproduction in the order Pleuronectiformes, we first cloned the LPXRFa gene, the piscine ortholog of GnIH, in the half-smooth tongue sole. The full-length cDNA of LPXRFa was 918bp in size with an open reading frame (ORF) of 585bp that encoded a 194 amino acids preprohormone with a calculated molecular mass and isoelectric point of 21.73kDa and 6.52, respectively. The LPXRFa precursor encoded two putative peptide sequences that included -MPMRF or -MPQRF motifs at the C-terminal. Tissue distribution analysis showed that LPXRFa transcripts could be detected at high levels in the brains of both sexes and to a lesser extent in the ovary, heart and stomach of females, while a noteworthy expression was observed in the kidney and muscle of males. Furthermore, the expression patterns of LPXRFa mRNA during ovarian maturation were also investigated. In the brain, the mRNA expression of LPXRFa increased significantly at stage III, declined at stage V and reached a maximum at stage VI. In the pituitary, the levels of LPXRFa mRNA remained stable during ovarian maturation and increased significantly to the top level at stage V and then declined back to basal levels. In contrast, the ovarian LPXRFa mRNA levels declined sharply at stage III and remained depressed over the course of ovarian maturation. Taken together, our results provide further evidence for the existence of LPXRFa in the order Pleuronectiformes and suggest its possible involvement in the regulation of reproduction in the female tongue sole.
- Published
- 2018
20. Associations of iron status with breast cancer risk factors in adult women: Findings from National Health and Nutrition Examination Survey 2017–2018
- Author
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Hong-Ye Chen, Xiao-Chong He, Lin Tian, Bao-Shi Bao, Yue Qiu, Yu-Hui Chen, and Xiao-Peng Hao
- Subjects
Adult ,Blood Glucose ,Male ,Waist ,National Health and Nutrition Examination Survey ,Iron ,Physiology ,Breast Neoplasms ,Biochemistry ,Body Mass Index ,Inorganic Chemistry ,Breast cancer ,Risk Factors ,medicine ,Humans ,Insulin ,Glycated Hemoglobin ,medicine.diagnostic_test ,biology ,Transferrin saturation ,business.industry ,nutritional and metabolic diseases ,Nutrition Surveys ,medicine.disease ,Ferritin ,Quartile ,Ferritins ,Serum iron ,biology.protein ,Transferrins ,Molecular Medicine ,Female ,Insulin Resistance ,Waist Circumference ,business ,Body mass index - Abstract
Objective This study examined the association between iron status and a set of breast cancer risk factors among U.S. adult women aged 20–80 years. Methods Data from National Health and Nutrition Examination Survey (2017–2018) were used to examine the relation between serum ferritin, serum iron and transferrin saturation with a set of breast cancer risk factors [body mass index (BMI), waist circumference, glycosylated hemoglobin (HbA1c), fasting plasma glucose, insulin and HOMA-IR]. The multivariable linear regressions were used controlling for age, race/ethnicity, menopause status, education level, smoking status, alcohol consumption, physical activity, high-sensitivity C-reactive protein (hsCRP) and total energy intake. Results HbA1c, BMI and waist circumference data were available for 1902 women with a fasting sample (n = 913) for fasting plasma glucose, insulin and HOMA-IR. Transferrin saturation had significant, inverse associations with BMI, waist circumference and HbA1c. The size of difference observed were that participants in the fourth quartile of transferrin saturation had a 4.50 kg/m2 smaller BMI, a 9.36 cm smaller waist circumference and a 0.1 % lower HbA1c level than participants in the first quartile. Similarly, serum iron concentrations were inversely associated with BMI and waist circumference. In addition, serum iron had significant, inverse associations with insulin and HOMA-IR. Sensitivity analyses among men gave similar results. For serum ferritin, there was a trend towards a positive association between waist circumference, HbA1c and fasting plasma glucose with serum ferritin. However, the associations did not reach statistical significance among women. Conclusions Iron status may impact breast cancer risk via effects on adiposity or glucose metabolism. The findings should be confirmed with further prospective data.
- Published
- 2021
21. Molecular characterization of kiss2 and differential regulation of reproduction-related genes by sex steroids in the hypothalamus of half-smooth tongue sole ( Cynoglossus semilaevis )
- Author
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Bao Shi, Quan Liu, Yongjiang Xu, Xuezhou Liu, Bin Wang, and Xuesong Song
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,DNA, Complementary ,Physiology ,Hypothalamus ,Ovary ,Gonadotropin-releasing hormone ,Biology ,Biochemistry ,Gonadotropin-Releasing Hormone ,03 medical and health sciences ,Kisspeptin ,Internal medicine ,medicine ,Animals ,Testosterone ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Receptor ,Molecular Biology ,Phylogeny ,Regulation of gene expression ,Kisspeptins ,Base Sequence ,Sequence Homology, Amino Acid ,Reproduction ,Estrogens ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Gene Expression Regulation ,Flatfishes ,Female ,hormones, hormone substitutes, and hormone antagonists ,Hormone - Abstract
Kisspeptin (Kiss) plays a critical role in mediating gonadal steroid feedback to the gonadotropin-releasing hormone (GnRH) neurons in mammals. However, little information regarding the regulation of kisspeptin gene by sex steroids is available in teleosts. In this study, we examined the direct actions of estradiol (E2) and testosterone (T) on hypothalamic expression of kisspeptin and other key factors involved in reproductive function of half-smooth tongue sole. As a first step, a partial-length cDNA of kiss2 was identified from the brain of tongue sole and kiss2 transcript levels were shown to be widely expressed in various tissues, notably in the ovary. Then, the actions of sex steroids on kiss2 and other reproduction-related genes were evaluated using a primary hypothalamus culture system. Our results showed that neither kiss2 nor its receptor kiss2r mRNA levels were significantly altered by sex steroids. Moreover, sex steroids did not modify hypothalamic expression of gonadotropin-inhibitory hormone (gnih) and its receptor gnihr mRNAs, either. However, E2 markedly stimulated both gnrh2 and gnrh3 mRNAs levels. Overall, this study provides insights into the role of sex steroids in the reproductive function of Pleuronectiform teleosts.
- Published
- 2017
22. Comprehensive CircRNA Profiling and Selection of Key CircRNAs Reveal the Potential Regulatory Roles of CircRNAs throughout Ovarian Development and Maturation in Cynoglossus semilaevis
- Author
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Bao Shi, Jing Li, Xuezhou Liu, Chongnv Wang, Changwei Shao, and Daiqiang Zhang
- Subjects
QH301-705.5 ,RNase R ,RNA-Seq ,Ovary ,Computational biology ,Biology ,Article ,General Biochemistry, Genetics and Molecular Biology ,reproduction ,symbols.namesake ,Cynoglossus semilaevis ,ovarian maturation ,medicine ,Biology (General) ,Gene ,Sanger sequencing ,General Immunology and Microbiology ,Gene ontology ,CircRNA ,medicine.anatomical_structure ,symbols ,RNA-seq ,General Agricultural and Biological Sciences ,Signalling pathways - Abstract
Simple Summary CircRNAs: as molecules involved in gene regulation, have become a new research hotspot in the non-coding RNA field. CircRNAs show tissue- or developmental stage-specific patterns of expression and can influence the expression levels of their parental genes. Recent studies have documented the potential biological roles of circRNAs in the growth, development, reproduction and health of humans and animals. Tongue sole (Cynoglossus semilaevis) is a marine flatfish that is an economically important farmed species in China. The commercial aquaculture of tongue sole has developed in the last few years because wild resources have decreased. Reproduction is regulated by brain-pituitary-gonad-liver axis which limits the development of artificial tongue sole culture. However, the roles of circRNAs in the ovarian development and maturation of tongue sole has never been reported. The identification of the potential functions of circRNAs provides a foundation for understanding the genetic mechanisms that regulate oocyte growth and maturation, which will allow the efficiency of tongue sole reproduction to be improved. Moreover, our findings extend the knowledge about a new type of endogenous RNA involved in regulating the ovarian development and maturation of tongue sole. Abstract CircRNAs are novel endogenous non-coding small RNAs involved in the regulation of multiple biological processes. However, little is known regarding circRNAs in ovarian development and maturation of fish. Our study, for the first time, provides the genome-wide overview of the types and relative abundances of circRNAs in tongue sole tissues during three ovarian developmental stages. We detected 6790 circRNAs in the brain, 5712 in the pituitary gland, 4937 in the ovary and 4160 in the liver. Some circRNAs exhibit tissue-specific expression, and qRT-PCR largely confirmed 6 differentially expressed (DE) circRNAs. Gene Ontology and KEGG pathway analyses of DE mRNAs were performed. Some DE circRNA parental genes were closely associated with biological processes in key signalling pathways and may play essential roles in ovarian development and maturation. We found that the selected circRNAs were involved in 10 pathways. RNase R digestion experiment and Sanger sequencing verified that the circRNA had a ring structure and was RNase R resistant. qRT-PCR results largely confirmed differential circRNA expression patterns from the RNA-seq data. These findings indicate that circRNAs are widespread in terms of present in production-related tissues of tongue sole with potentially important regulatory roles in ovarian development and maturation.
- Published
- 2021
23. Inhibitory action of tongue sole LPXRFa, the piscine ortholog of gonadotropin-inhibitory hormone, on the signaling pathway induced by tongue sole kisspeptin in COS-7 cells transfected with their cognate receptors
- Author
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Quan Liu, Bao Shi, Xuezhou Liu, Yongjiang Xu, Jingkai Qin, Guokun Yang, Bin Wang, and Wensheng Li
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Physiology ,Gonadotrophs ,Biology ,Transfection ,Gonadotropic cell ,Biochemistry ,Gonadotropin-Releasing Hormone ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Endocrinology ,Kisspeptin ,Internal medicine ,Chlorocebus aethiops ,medicine ,Animals ,Protein kinase A ,Receptor ,Protein Kinase C ,Protein kinase C ,Neurons ,Kisspeptins ,Phospholipase C ,Reproduction ,Fishes ,Cyclic AMP-Dependent Protein Kinases ,Cell biology ,Intracellular signal transduction ,030104 developmental biology ,Gene Expression Regulation ,Type C Phospholipases ,COS Cells ,Signal transduction ,Gonadotropins ,hormones, hormone substitutes, and hormone antagonists ,Adenylyl Cyclases ,Signal Transduction - Abstract
Kisspeptin (Kiss) acts as a positive regulator of reproduction by acting on gonadotropes and gonadotropin-releasing hormone (GnRH) neurons. Despite its functional significance, the intricate web of intracellular signal transduction pathways in response to Kiss is still far from being fully understood in teleosts. Accordingly, we investigated the molecular mechanism of Kiss action and its possible interaction with LPXRFa signaling in this study. In vitro functional analysis revealed that synthetic tongue sole Kiss2 decapeptide increased the cAMP responsive element-dependent luciferase (CRE-luc) activity in COS-7 cells transfected with its cognate receptor, while this stimulatory effect was markedly reduced by two inhibitors of the adenylate cyclase (AC)/protein kinase A (PKA) pathway. Similarly, Kiss2 also significantly stimulated serum responsive element-dependent luciferase (SRE-luc) activity, whereas this stimulatory effect was evidently attenuated by two inhibitors of the phospholipase C (PLC)/protein kinase C (PKC) pathway. In addition, LPXRFa-2 suppressed Kiss2-elicited CRE-luc activity in a dose-dependent manner. Taken together, Kiss2 utilizes both AC/PKA and PLC/PKC pathways to exert its functions via its cognate receptor and LPXRFa may antagonize the action of Kiss2 by inhibiting kisspeptin signaling. As far as we know, this study is the first to characterize the half-smooth tongue sole kisspeptin and LPXRFa signaling pathway in COS-7 cells transfected with their cognate receptors and provides novel information on the interaction between LPXRFa system and kisspeptin system in teleosts.
- Published
- 2017
24. Molecular characterization of Kiss2 receptor and in vitro effects of Kiss2 on reproduction-related gene expression in the hypothalamus of half-smooth tongue sole ( Cynoglossus semilaevis )
- Author
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Bao Shi, Yongjiang Xu, Bin Wang, Quan Liu, and Xuezhou Liu
- Subjects
0301 basic medicine ,endocrine system ,medicine.medical_specialty ,DNA, Complementary ,Hypothalamus ,Receptors, Cell Surface ,Biology ,03 medical and health sciences ,Endocrinology ,Kisspeptin ,Tongue ,Internal medicine ,Complementary DNA ,Gene expression ,medicine ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Sexual Maturation ,Cloning, Molecular ,Receptor ,Phylogeny ,Kisspeptins ,Base Sequence ,Gene Expression Profiling ,Reproduction ,In vitro ,030104 developmental biology ,medicine.anatomical_structure ,Gene Expression Regulation ,Flatfishes ,Female ,Animal Science and Zoology ,Sequence Alignment ,Smooth tongue - Abstract
Kisspeptin (Kiss) and its receptor, KissR (previously known as GPR54), play a critical role in the control of reproduction and puberty onset in mammals. Additionally, a number of studies have provided evidence of the existence of multiple Kiss/KissR systems in teleosts, but the physiological relevance and functions of these kisspeptin forms (Kiss1 and Kiss2) still remain to be investigated. To this end, we examined the direct actions of Kiss2 on hypothalamic functions in the half-smooth tongue sole (Cynoglossus semilaevis), a representative species of the order Pleuronectiformes. As a first step, the full-length cDNA for kiss2r was identified and kiss2r transcripts were shown to be widely expressed in various tissues, notably in the brain of tongue sole. Then, the effects of Kiss2 decapeptide on reproduction-related gene expression were evaluated using a primary hypothalamus culture system. Our results showed that neither gnrh2 nor gnrh3 mRNA levels were altered by Kiss2. However, Kiss2 significantly increased the amounts of gnih and kiss2 mRNAs. In contrast, Kiss2 elicited an evident inhibitory effect on both gnihr and kiss2r mRNA levels. To the best of our knowledge, this is the first description of a direct and differential regulation of reproduction-related gene expression by Kiss2 at the hypothalamus level of a teleost fish. Overall, this study provides novel information on the role of Kiss2/Kiss2R system in the reproductive function of teleosts.
- Published
- 2017
25. Association of obesity with Helicobacter pylori infection: A retrospective study
- Author
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Mei-Yan Xu, Qing-Bin Lu, Lan Liu, Bao-Shi Yuan, and Jian Yin
- Subjects
Adult ,Male ,medicine.medical_specialty ,China ,Overweight ,Logistic regression ,Gastroenterology ,Body Mass Index ,Helicobacter Infections ,03 medical and health sciences ,0302 clinical medicine ,Retrospective Study ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Obesity ,Retrospective Studies ,biology ,Helicobacter pylori ,business.industry ,Confounding ,Retrospective cohort study ,General Medicine ,Middle Aged ,biology.organism_classification ,medicine.disease ,bacterial infections and mycoses ,030211 gastroenterology & hepatology ,Female ,medicine.symptom ,business ,Body mass index ,Weight gain - Abstract
Aim To explore the association between Helicobacter pylori (H. pylori) infection and obesity/weight gain in a Chinese population. Methods Our primary outcome was the change in body mass index (BMI). The generalized linear models were used to explore the association between H. pylori infection and the change of BMI, and the logistic regression models were used to explore the association between H. pylori infection and obesity. Results A total of 3039 subjects were recruited and analyzed, of which 12.8% were obese. The prevalence of H. pylori infection was 53.9% (1639/3039) overall and 54.6% (212/388) in the obese subjects. The change of BMI in the H. pylori (+) group was not significantly higher than that in the H. pylori (-) group after adjustment for potential confounding factors [RR = 0.988, 95%CI: 0.924-1.057, P = 0.729]. The prevalence of obesity decreased 1.1% in the H. pylori (+) group and 0.5% in the H. pylori (-) group. The RR of H. pylori infection for obesity was 0.831 (95%CI: 0.577-1.197, P = 0.321) after the adjustment. Conclusion H. pylori infection was not associated with overweight/obesity observed from the retrospective study in this Chinese population.
- Published
- 2017
26. Evidences for involvement of growth hormone and insulin-like growth factor in ovarian development of starry flounder (Platichthys stellatus)
- Author
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Xuezhou Liu, Yongjiang Xu, Bin Wang, Kun Zang, and Bao Shi
- Subjects
Fish Proteins ,0301 basic medicine ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,030209 endocrinology & metabolism ,Flounder ,Aquatic Science ,Biochemistry ,03 medical and health sciences ,Paracrine signalling ,Basal (phylogenetics) ,Insulin-like growth factor ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Testosterone ,RNA, Messenger ,Insulin-Like Growth Factor I ,Autocrine signalling ,Starry flounder ,Estradiol ,biology ,Growth factor ,Ovary ,Gene Expression Regulation, Developmental ,General Medicine ,biology.organism_classification ,Gonadosomatic Index ,030104 developmental biology ,Endocrinology ,Liver ,Growth Hormone ,Female - Abstract
Although gonadotrophins are major regulators of ovarian function in teleosts and other vertebrates, accumulating evidence indicates that the growth hormone (GH)-insulin-like growth factor (IGF) axis also plays an important role in fish reproduction. As a first step to understand the physiological role of the GH-IGF system in the ovarian development of starry flounder (Platichthys stellatus), the expression profiles of GH and IGF messenger RNAs (mRNAs) and plasma GH, IGF-I, estradiol-17β (E2), and testosterone (T) levels during the ovarian development were investigated. The developmental stages of ovaries were divided into five stages (II, III, IV, V, and VI) by histological analysis. The hepatosomatic index (HSI) and gonadosomatic index (GSI) values increased and peaked at stage IV and stage V, respectively, and then declined at stage VI. Pituitary GH mRNA levels decreased sharply at stage III and raised to top level at stage VI. The hepatic IGF-I mRNA levels ascended to maximum value at stage V and then declined significantly at stage VI. However, the hepatic IGF-II mRNA levels remained stable and increased significantly at stage VI. In contrast, the ovarian IGF-I mRNA levels increased gradually and peaked at stage VI. The ovarian IGF-II mRNA levels were initially stable and increased significantly at stage V until the top level at stage VI. Consistent with the pituitary GH mRNA levels, plasma GH levels reduced sharply at stage III and remained depressed until stage V and then raised remarkably at stage VI. Plasma IGF-I level peaked at stage V and then declined to initial level. Plasma E2 level peaked at stage IV and then dramatically descended to the basal level. Plasma T level peaked at stage V and then declined significantly back to the basal level. Based on statistical analysis, significant positive correlations between hepatic IGF-I mRNA and GSI, ovarian IGF-II mRNA and hepatic IGF-II mRNA, ovarian IGF-I mRNA and ovarian IGF-II mRNA, and plasma IGF-I and plasma T were observed, respectively. These results suggest that the GH-IGF system may be involved in the ovarian development of starry flounder; GH and IGFs appear to play distinct roles in the regulation of the ovarian development in paracrine/autocrine manners. These findings extend our knowledge of the roles of the GH-IGF axis on reproduction regulation in fish.
- Published
- 2016
27. Naringenin inhibits spinal cord injury-induced activation of neutrophils through miR-223
- Author
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Peifu Tang, Long-Bao Shi, Wei Zhang, Licheng Zhang, Hao Zhang, and Zhao Yanpeng
- Subjects
Male ,0301 basic medicine ,Naringenin ,medicine.medical_specialty ,Neutrophils ,medicine.medical_treatment ,Interleukin-1beta ,Anti-Inflammatory Agents ,Biology ,Neutrophil Activation ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Injury Site ,mir-223 ,Internal medicine ,NLR Family, Pyrin Domain-Containing 3 Protein ,Genetics ,medicine ,Animals ,Spinal cord injury ,Saline ,Spinal Cord Injuries ,Neuroinflammation ,Laminectomy ,General Medicine ,medicine.disease ,Spinal cord ,Rats ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Flavanones ,Immunology ,030217 neurology & neurosurgery - Abstract
Naringenin (NR), a flavonoid abundant in citrus fruits has been reported to possess anti-inflammatory properties. The present study aimed to investigate the protective of naringenin in rats after spinal cord injury (SCI) and the underlying mechanisms associated with neuroinflammation. Adult male Sprague-Dawley rats were subjected to laminectomy at T9-T11 and compression with a vascular clip. The spinal cords spanning the injury site about 0.8cm were collected for testing. There were five groups (n=7 in each group): (a) Control group; (b) sham group group; (c) SCI+saline; (d) SCI+NR (50mg/kg, p.o.) group and (e) SCI+NR (100mg/kg, p.o.) group. Different doses of NR (50mg/kg, p.o. and 100mg/kg, p.o.) or saline were administered once daily for 11 consecutive days, from 3days prior to surgery to 7days after surgery. The expression level of miR-223, NLRP3 and IL-1β were measured by RT- qPCR. The accumulation of neutrophils at the site of compression, as evaluated by measuring the tissue myeloperoxidase activity, significantly increased with time following the compression, peaking at 24h post compression. The expression of miR-223 was significant elevated in (b). However, spinal cord myeloperoxidase activity and the expression of miR-223 did not increase in sham-operated animals. NR significantly inhibited a SCI-induced activation of neutrophils through repressed miR-223 in group (d) and (e). There was a better effect in group (e) than group (d). miR-223 is thought to act as a fine-tuner of granulocyte production and the inflammatory response. Our findings suggested that repeated administration of naringenin (100mg/kg, p.o) may provide the protective effect of the spinal cord injury in rats, possibly through inhibiting neuroinflammation.
- Published
- 2016
28. Whole-transcriptome sequencing profiling identifies functional and prognostic signatures in patients with PTPRZ1-MET fusion-negative secondary glioblastoma multiforme
- Author
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Shu-Qing Yu, Guanzhang Li, Zhaoshi Bao, Zhiliang Wang, Chuanbao Zhang, Bao‑Shi Chen, and Kuanyu Wang
- Subjects
0301 basic medicine ,Oncology ,Cancer Research ,medicine.medical_specialty ,PTPRZ1-MET fusion ,Brain tumor ,Protein tyrosine phosphatase ,risk score ,Receptor tyrosine kinase ,immune response ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Glioma ,glioma ,medicine ,Oncogene ,biology ,business.industry ,secondary glioblastoma multiforme ,Articles ,Cell cycle ,medicine.disease ,Molecular medicine ,030104 developmental biology ,030220 oncology & carcinogenesis ,PTPRZ1 ,biology.protein ,business - Abstract
Gliomas are the most common type of primary brain tumor in adults with a high mortality rate. Low-grade gliomas progress to glioblastoma multiforme (GBM) in the majority of cases, forming secondary GBM (sGBM), followed by rapid fatal clinical outcomes. Protein tyrosine phosphatase receptor type Z1 (PTPRZ1)-MET proto-oncogene receptor tyrosine kinase (MET) (ZM) fusion has been identified as a biomarker for sGBM that is involved in glioma progression, but the mechanism of gliomagenesis and pathology of ZM-negative sGBM has remained to be fully elucidated. A whole-transcriptome signature is thus required to improve the outcome prediction for patients with sGBM without ZM fusion. In the present study, whole-transcriptome sequencing on 42 sGBM samples with or without ZM fusion from the Chinese Glioma Genome Atlas database identified mRNAs with differential expression between patients with and without ZM fusion and the most significant survival-associated genes were identified. A 6-gene signature was identified as a novel prognostic model reflecting survival probability in patients with ZM-negative sGBM. Clinical characteristics in patients with a high or low risk score value were analyzed with the Kaplan-Meier method and a two-sided log-rank test. In addition, ZM-negative sGBM patients with a high risk score exhibited an increase in immune cells, NF-κB-induced pathway activation and a decrease in endothelial cells compared with those with a low risk score. The present study demonstrated the potential use of a next-generation sequencing-based cancer gene signature in patients with ZM-negative sGBM, indicating possible clinical therapeutic strategies for further treatment of such patients.
- Published
- 2019
29. Novel Pyrazolo[4,3- d]pyrimidine as Potent and Orally Active Inducible Nitric Oxide Synthase (iNOS) Dimerization Inhibitor with Efficacy in Rheumatoid Arthritis Mouse Model
- Author
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Ming Ming Liu, Xin Huang, Xin Hua Liu, Liu Zeng Chen, Ming Ming Jiao, Wen Jian Tang, Bao Shi Wang, and Jing Bo Shi
- Subjects
Lipopolysaccharides ,Pyrimidine ,Pyridines ,Interleukin-1beta ,Anti-Inflammatory Agents ,Molecular Conformation ,Arthritis ,Nitric Oxide Synthase Type II ,Pharmacology ,Inhibitory postsynaptic potential ,Crystallography, X-Ray ,Nitric Oxide ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,Structure-Activity Relationship ,Drug Discovery ,medicine ,Animals ,Enzyme Inhibitors ,IC50 ,030304 developmental biology ,0303 health sciences ,Low toxicity ,biology ,Macrophages ,medicine.disease ,Arthritis, Experimental ,0104 chemical sciences ,Rats ,Nitric oxide synthase ,010404 medicinal & biomolecular chemistry ,Disease Models, Animal ,Orally active ,RAW 264.7 Cells ,chemistry ,Rheumatoid arthritis ,Drug Design ,biology.protein ,Molecular Medicine ,Pyrazoles ,Dimerization - Abstract
In order to discover novel anti-inflammatory agents for treatment of arthritis and based on preliminary structure–activity relationships, four series (A–D) of total 90 new pyrazolo[4,3-d]pyrimidine compounds were designed and synthesized. All the compounds have been tested for their anti-inflammatory activities by inhibiting of LPS-induced NO production. A clear structure–activity relationship has been concluded step by step, and finally 3,4,5-trimethoxystyryl-1H-pyrazolo[4,3-d]pyrimidine was found to be the most active scaffold. Among them, compound D27 was discovered as the most potent anti-inflammatory agent (IC50 = 3.17 μM) with low toxicity and strong inhibitory of NO release (IR = 90.4% at 10 μM). This compound also showed potent inhibition of iNOS with IC50 value of 1.12 μM. Preliminary mechanism studies indicated that it could interfere with the stability and formation of active dimeric iNOS. The anti-inflammatory effect of this compound was determined by adjuvant-induced arthritis in rat model. W...
- Published
- 2019
30. Four new species of the genus Mallinella (Araneae: Zodariidae) from Malaysia
- Author
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Feng Zhang and Bao-Shi Zhang
- Subjects
Species groups ,food.ingredient ,Natural forest ,Malaysia ,Zoology ,Mallinella ,Spiders ,Biology ,Forests ,food ,Animals ,Animal Science and Zoology ,Taxonomy (biology) ,Ecology, Evolution, Behavior and Systematics - Abstract
Four new species of the genus Mallinella Strand, 1906, from the natural forests of Malaysia, are described as Mallinella bicanaliculata sp. n. (♂♀), M. calautica sp. n. (♂♀), M. laxa sp. n. (♂♀), and M. obliqua sp. n. (♂♀). The four new species belong to four species groups and were collected from the forest litter in Sabah state by sieving.
- Published
- 2019
31. Three new species of the genus Zodarion (Araneae, Zodariidae) from China
- Author
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Feng Zhang and Bao-Shi Zhang
- Subjects
0106 biological sciences ,Asia ,Zodarion ,Arthropoda ,italicum group ,010607 zoology ,Nephrozoa ,Zoology ,Protostomia ,010603 evolutionary biology ,01 natural sciences ,Circumscriptional names of the taxon under ,taxonomy ,lcsh:Zoology ,Arachnida ,Thelyphonida ,Animalia ,Bilateria ,lcsh:QL1-991 ,China ,Ecology, Evolution, Behavior and Systematics ,zodariides ,biology ,Cephalornis ,biology.organism_classification ,lutipes group ,Notchia ,Araneae ,Ecdysozoa ,Animal Science and Zoology ,Taxonomy (biology) ,Chasmataspidida ,Zodariidae ,Coelenterata - Abstract
Three new species of the genus Zodarion Walckenaer, 1826, from China, are described as Zodarionapertumsp. n. (♂♀, from Xinjiang), Z.planumsp. n. (♂, from Shaanxi), and Z.ovatumsp. n. (♂♀, from Yunnan).
- Published
- 2019
32. Correlation between apparent diffusion coefficient and pathological characteristics of patients with invasive breast cancer
- Author
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Wuyao Yang, Hong-Ye Chen, Jian-Dong Wang, Yue Qiu, Yu-Hui Chen, Xiuxiu Zhang, Lin Tian, and Bao-Shi Bao
- Subjects
medicine.medical_specialty ,biology ,Receiver operating characteristic ,business.industry ,Urology ,Estrogen receptor ,General Medicine ,medicine.disease ,Confidence interval ,Benign tumor ,03 medical and health sciences ,0302 clinical medicine ,Breast cancer ,030220 oncology & carcinogenesis ,Ki-67 ,medicine ,biology.protein ,Effective diffusion coefficient ,Original Article ,030212 general & internal medicine ,business ,Pathological - Abstract
BACKGROUND: There is insufficient research on the correlation between the apparent diffusion coefficient and clinicopathological characteristics of breast cancer patients. The present study is to investigate the correlation between the apparent diffusion coefficient and pathological characteristics of patients with invasive breast cancer. METHODS: From January 2019 to September 2020, 122 cases of invasive breast cancer and 21 cases of benign tumors were retrospectively enrolled. The apparent diffusion coefficient was compared between the two groups, and the correlation between the apparent diffusion coefficient and the pathological characteristics of the patients with invasive breast cancer were analyzed. RESULTS: Compared with the benign tumor group, the apparent diffusion coefficient in the invasive breast cancer group was significantly lower (0.89±0.17 vs. 1.47±0.27 10(−3) mm(2)/s, P=0.000). Using the apparent diffusion coefficient to diagnose patients with invasive breast cancer, the area under receiver operating characteristic (ROC) curve was 0.966±0.021 [95% confidence interval (CI): 0.924–1.000, P=0.000], and the best diagnostic cut-off value was 1.16 (10(−3) mm(2)/s), with sensitivity and specificity of 0.905 and 0.902, respectively. The apparent diffusion coefficient was used to diagnose vascular tumor thrombus in patients with invasive breast cancer. The area under the ROC curve was 0.641±0.068 (95% CI: 0.508–0.774, P=0.047), and the best diagnostic threshold was 0.835 (10(−3) mm(2)/s), with sensitivity and specificity of 0.676 and 0.650, respectively. The apparent diffusion coefficient in patients with high expression of Ki-67 (%) was significantly reduced (0.87±0.17 vs. 1.00±0.16 10(−3) mm(2)/s, P=0.000). The apparent diffusion coefficient was not significantly correlated with age, menopause, lesion size, estrogen receptor, progesterone receptor, or lymph node metastasis in patients with invasive breast cancer (P>0.05). CONCLUSIONS: In patients with invasive breast cancer the apparent diffusion coefficient was significantly reduced. It was able to differentiate invasive breast cancer and vascular tumor thrombus, and was also related to Ki-67 (%) high expression.
- Published
- 2021
33. Dimeric and monomeric dihydrostilbenes from the roots of Stemona mairei together with their hypoglycemic activities
- Author
-
Ying Zeng, Bao-Bao Shi, Fang Wang, Xiang-Hai Cai, Gao Chen, and Johann Schinnerl
- Subjects
Quantum chemical ,Stemona mairei ,biology ,Chemistry ,Stereochemistry ,Chemical shift ,Organic Chemistry ,Absolute configuration ,biology.organism_classification ,Biochemistry ,chemistry.chemical_compound ,Monomer ,Phytochemical ,Drug Discovery ,Stemonaceae ,Plant species - Abstract
A carefully performed phytochemical investigation of the roots of Stemona mairei (Stemonaceae) led to the discovery of six so far undescribed dihydrostilbene dimers maistilbene A–F and four monomers maistilbene G–J. This is the first report of stilbenoids of this plant species and the first report of dimeric dihydrostilbenes from this plant family. The absolute configuration of these compounds was determined by a combination of Mo2(AcO)4-induced ECD spectra and quantum chemical calculation of NMR chemical shift, ECD and spin-spin coupling constants. Maistilbene G could increase the glucose consumption in L6 myotubes, disclosing a new hypoglycemic candidate.
- Published
- 2021
34. miR-124 and Androgen Receptor Signaling Inhibitors Repress Prostate Cancer Growth by Downregulating Androgen Receptor Splice Variants, EZH2, and Src
- Author
-
Xu Bao Shi, Ralph W deVere White, Clifford G. Tepper, Regina F Gandour-Edwards, Hsing Jien Kung, Christopher P. Evans, Joy C. Yang, Hao G. Nguyen, Lingru Xue, Meimei Li, and Ai Hong Ma
- Subjects
Male ,Cancer Research ,Androgen ,Mice ,chemistry.chemical_compound ,Prostate cancer ,Receptors ,2.1 Biological and endogenous factors ,Protein Isoforms ,Aetiology ,Cancer ,Tumor ,Prostate Cancer ,EZH2 ,Polycomb Repressive Complex 2 ,Gene Expression Regulation, Neoplastic ,src-Family Kinases ,Oncology ,5.1 Pharmaceuticals ,Receptors, Androgen ,Development of treatments and therapeutic interventions ,Signal transduction ,Biotechnology ,Signal Transduction ,Proto-oncogene tyrosine-protein kinase Src ,Urologic Diseases ,Oncology and Carcinogenesis ,Down-Regulation ,Biology ,Article ,Cell Line ,Downregulation and upregulation ,Cell Line, Tumor ,microRNA ,Genetics ,medicine ,Animals ,Humans ,Enzalutamide ,Enhancer of Zeste Homolog 2 Protein ,Oncology & Carcinogenesis ,Neoplastic ,Prostatic Neoplasms ,medicine.disease ,Xenograft Model Antitumor Assays ,Molecular biology ,Androgen receptor ,MicroRNAs ,Gene Expression Regulation ,chemistry ,Cancer research - Abstract
miR-124 targets the androgen receptor (AR) transcript, acting as a tumor suppressor to broadly limit the growth of prostate cancer. In this study, we unraveled the mechanisms through which miR-124 acts in this setting. miR-124 inhibited proliferation of prostate cancer cells in vitro and sensitized them to inhibitors of androgen receptor signaling. Notably, miR-124 could restore the apoptotic response of cells resistant to enzalutamide, a drug approved for the treatment of castration-resistant prostate cancer. We used xenograft models to examine the effects of miR-124 in vivo when complexed with polyethylenimine-derived nanoparticles. Intravenous delivery of miR-124 was sufficient to inhibit tumor growth and to increase tumor cell apoptosis in combination with enzalutamide. Mechanistic investigations revealed that miR-124 directly downregulated AR splice variants AR-V4 and V7 along with EZH2 and Src, oncogenic targets that have been reported to contribute to prostate cancer progression and treatment resistance. Taken together, our results offer a preclinical rationale to evaluate miR-124 for cancer treatment. Cancer Res; 75(24); 5309–17. ©2015 AACR.
- Published
- 2015
35. Preferential degradation of long-chain alkyl substituted hydrocarbons in heavy oil under methanogenic conditions
- Author
-
Bo Tu, Lai yan Liu, Jan Dolfing, Qiang Li, Lei Cheng, Sheng bao Shi, Yahe Zhang, Yong ge Sun, Lu Yang, Lirong Dai, Hui Zhang, and Quan Shi
- Subjects
chemistry.chemical_classification ,010504 meteorology & atmospheric sciences ,biology ,chemistry.chemical_element ,Methanosarcina ,Methanothrix ,Biodegradation ,010502 geochemistry & geophysics ,biology.organism_classification ,01 natural sciences ,Methane ,chemistry.chemical_compound ,chemistry ,Geochemistry and Petrology ,Environmental chemistry ,Degradation (geology) ,Carbon ,Alkyl ,0105 earth and related environmental sciences ,Asphaltene - Abstract
Methanogenic crude oil degradation is a significant process in subsurface environments and degradation of crude oil n-alkanes has been well documented. However, little is known about the biodegradability of the resulting heavy oil. In this study, a methanogenic consortium enriched from Shengli oilfield generated 1.3–1.9 mmol CH4/g of heavy oil at a rate of 2.9–8.8 μmol CH4/g of oil/day. Four SARA fractions (saturates, aromatics, resins and asphaltenes) of oils experienced a loss in linear aliphatic structures. n-Alkylcyclohexanes, methyl-n-alkylcyclohexanes, n-alkyldecalins, n-alkylbenzenes, n-alkyltoluenes and n-alkylxylenes with alkyl side chains longer than 14 carbons were degraded over 50% compared to the undegraded oil. In addition, the extent of degradation of these hydrocarbons increased with increasing carbon length. Correspondingly, n-fatty acids and naphthenic acids with 1–3 naphthenic rings accumulated over time. 16S rRNA gene analysis revealed that aceticlastic Methanosarcina and Methanothrix dominated in the archaeal domain, and bacterial members related to Dehalococcoidia and Soehngenia were consistently present in the successive transfer cultures. However, neither assA/masD-like genes nor alkyl-substituted succinate metabolites were detected, indicating an alternative degradation pathway, rather than addition to fumarate. This study provides novel insights into methanogenic degradation of long-chain alkyl substituted hydrocarbons in heavy oil, which also extends our understanding of anaerobic degradation of crude oil in subsurface sedimentary environments.
- Published
- 2019
36. Two new species of the spider Family Zodariidae from Laos
- Author
-
Feng Zhang and Bao-Shi Zhang
- Subjects
Male ,Spider ,food.ingredient ,Arthropoda ,Natural forest ,Zoology ,Mallinella ,Spiders ,Biodiversity ,Biology ,Forests ,food ,Laos ,Arachnida ,Animals ,Animalia ,Araneae ,Animal Science and Zoology ,Taxonomy (biology) ,Female ,Animal Distribution ,Zodariidae ,Ecology, Evolution, Behavior and Systematics ,Taxonomy - Abstract
Two new Zodariidae species, collected from the natural forest of Pakse City, Champasak Province, Laos, are described as Asceua adunca sp. nov. (male, female) and Mallinella renaria sp. nov. (male, female). Asceua torquata (Simon, 1909) and A. piperata Ono, 2004 are newly recorded from Laos, and the male of A. piperata is described and illustrated for the first time.
- Published
- 2018
37. Characterization of LPXRFa receptor in the half-smooth tongue sole (Cynoglossus semilaevis): Molecular cloning, expression profiles, and differential activation of signaling pathways by LPXRFa peptides
- Author
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Xuezhou Liu, Quan Liu, Yongjiang Xu, Guokun Yang, Bao Shi, Bin Wang, Jingkai Qin, and Wensheng Li
- Subjects
0301 basic medicine ,DNA, Complementary ,Physiology ,Neuropeptide ,Biology ,Molecular cloning ,Real-Time Polymerase Chain Reaction ,Biochemistry ,03 medical and health sciences ,Open Reading Frames ,Complementary DNA ,Chlorocebus aethiops ,Animals ,Amino Acid Sequence ,RNA, Messenger ,Cloning, Molecular ,Receptor ,Molecular Biology ,Protein kinase C ,Phylogeny ,Hypothalamic Hormones ,Base Sequence ,Sequence Homology, Amino Acid ,Gene Expression Profiling ,Transfection ,Cell biology ,Open reading frame ,030104 developmental biology ,COS Cells ,Flatfishes ,Female ,Signal transduction ,Peptides ,Signal Transduction - Abstract
Gonadotropin-inhibitory hormone (GnIH), a novel hypothalamic neuropeptide, serves as a key player in the regulation of reproduction across vertebrates, acting on the brain and pituitary to modulate reproductive physiology and behavior. However, little information is available in teleosts regarding the intracellular signal transduction pathway in response to GnIH. To this end, we first cloned the gene of LPXRFa (the piscine ortholog of GnIH) receptor in the half-smooth tongue sole ( Cynoglossus semilaevis ), a representative species of the order Pleuronectiformes. The full-length cDNA of LPXRFa receptor was 2201 bp in size with an open reading frame (ORF) of 1365 bp that encoded 454 amino acids. Tissue distribution showed that LPXRFa receptor transcripts could be detected at high levels in the brain, to a lesser extent in the pituitary, and at low levels in the ovary and other peripheral tissues. In vitro functional analysis revealed that putative tongue sole LPXRFa-1 and LPXRFa-2 peptides significantly stimulated serum responsive element-dependent luciferase (SRE-luc) activity in COS-7 cells transfected with the novel receptor, and these stimulatory effects were evidently reduced by two inhibitors of the PLC/PKC pathway. In addition, neither LPXRFa-1 nor LPXRFa-2 altered the cAMP-responsive element (CRE)-luc activity, but only LPXRFa-2 could markedly decrease forskolin-induced CRE-luc activity in COS-7 cells expressing its cognate receptor. Taken together, our results encompass the first study reporting the existence of LPXRFa receptor in the order Pleuronectiformes and provide novel evidence of differential activation of signaling pathways by LPXRFa peptides in fish.
- Published
- 2018
38. Characterization of the complete chloroplast genome of Gerbera jamesonii Bolus in China and phylogenetic relationships
- Author
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Xiao-bao Shi, Fan Liu, Xing-qi Wang, Zhongxiong Lai, Zhang Yongyan, Na Tian, and Chunzhen Cheng
- Subjects
0106 biological sciences ,0301 basic medicine ,Gerbera ,Gerbera jamesonii Bolus ,Compositae ,biology ,Phylogenetic tree ,phylogenetic relationship analysis ,Cut flowers ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Genome ,Chloroplast ,03 medical and health sciences ,030104 developmental biology ,Bolus (medicine) ,Gerbera jamesonii ,Botany ,Ornamental plant ,Genetics ,chloroplast genome ,Molecular Biology ,Mitogenome Announcement ,Research Article - Abstract
Gerbera (Gerbera jamesonii Bolus) is one of the most popular ornamental flowers and one of the top five cut flowers worldwide. In this study, we presented the complete chloroplast genome of it using BGISEQ-500 sequencing. Its chloroplast genome is 151,898 bp in size, containing a large single copy region (83,518 bp) and a small single copy region (18,244 bp), and a pair of IR regions (25,068 bp). The chloroplast genome contains 113 unique genes, including 80 protein-coding genes, 29 tRNAs, and 4 rRNAs. Phylogenetic maximum likelihood analysis based on chloroplast genomes of Gerbera and other 17 plant species indicated that the relationship between Gerbera and other plant species of family Compositae is not very close and its relationship with Atractylodes lancea is the closest.
- Published
- 2019
39. Complete mitochondrial genome of yellowtail kingfish Seriola aureovittata (Perciformes, Carangidae)
- Author
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Bao Shi, Xuezhou Liu, Yongjiang Xu, Yan Jiang, and Bin Wang
- Subjects
0106 biological sciences ,0301 basic medicine ,Genetics ,Mitochondrial DNA ,biology ,Seriola ,biology.organism_classification ,010603 evolutionary biology ,01 natural sciences ,Perciformes ,03 medical and health sciences ,030104 developmental biology ,Carangidae ,%22">Fish ,Molecular Biology ,Sequence (medicine) - Abstract
The complete mitochondrial genomic sequence of Yellowtail Kingfish Seriola aureovittata was determined in this study. The circular mitogenome contains 16,609 bp, encoding 37 typical animal mitochon...
- Published
- 2019
40. Association of anaemia with Helicobacter pylori infection: a retrospective study
- Author
-
Bing Cao, Jian Yin, Qing-Bin Lu, Mei-Yan Xu, Bao-Shi Yuan, and Lan Liu
- Subjects
Adult ,medicine.medical_specialty ,Helicobacter pylori infection ,China ,Anemia ,Population ,lcsh:Medicine ,Registration system ,Logistic regression ,Gastroenterology ,Article ,Helicobacter Infections ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,education ,lcsh:Science ,Aged ,education.field_of_study ,Multidisciplinary ,biology ,Helicobacter pylori ,business.industry ,lcsh:R ,Retrospective cohort study ,Middle Aged ,medicine.disease ,biology.organism_classification ,bacterial infections and mycoses ,Immunology ,030211 gastroenterology & hepatology ,lcsh:Q ,business ,Body mass index - Abstract
The role of Helicobacter pylori (H. pylori) infection in haematological system diseases is not well understood. We conducted this retrospective study to explore the association between H. pylori infection and anaemia in the Chinese population. This retrospective study was performed in Aerospace Center Hospital in Beijing. We derived the data from the registration system of the physical population between 2012–2016. Logistic regression models were used to explore the association between H. pylori infection and anaemia. Among 17,791 subjects, there were 7,804 (43.9%) subjects with H. pylori infection and 950 (5.3%) with anaemia. The prevalence of anaemia in the H. pylori (+) group was significantly higher than in the H. pylori (−) group after adjusting for age, sex, marriage, underlying diseases and body mass index. Compared to H. pylori (−), the OR of H. pylori (+) was 1.39 for moderate-to-severe anaemia and 1.05 for mild anaemia. The level of haemoglobin was lower in the H. pylori (+) group than in the H. pylori (−) group. This study indicates that H. pylori infection may be related to anaemia and haemoglobin level in the Chinese population.
- Published
- 2017
41. Molecular and transcriptional characterization ofGTHsandmPRαduring ovarian maturation in rock breamOplegnathus fasciatus
- Author
-
Yongjiang Xu, Bao Shi, Xuezhou Liu, Sun Zhongzhi, Kun Zang, and Shengyi Chen
- Subjects
medicine.medical_specialty ,Physiology ,medicine.drug_class ,Ovary ,Biology ,Oocyte ,Andrology ,Gonadosomatic Index ,Endocrinology ,medicine.anatomical_structure ,Internal medicine ,Gene expression ,Genetics ,medicine ,Animal Science and Zoology ,Vitellogenesis ,Gonadotropin ,Receptor ,Molecular Biology ,Ecology, Evolution, Behavior and Systematics ,Testosterone - Abstract
The membrane progestin receptor α (mPRα) plays an important role in the regulation of oocyte meiotic maturation in fish. However, the endocrine regulation of multiple spawning in fish with asynchronous ovarian development remains to be determined. We identified four genes (FSHβ, LHβ, CGα and mPRα) that are widely expressed in both the reproductive and non-reproductive tissues of rock bream Oplegnathus fasciatus and examined their expression during ovarian maturation. The level of FSHβ transcripts in the pituitary remained high during vitellogenic stages, when the plasma levels of estradiol-17β reached maximum levels and oocyte development was stimulated. LHβ expression in the pituitary and ovary reached maximum levels during oocyte maturation when the plasma levels of testosterone also reached a maximum. A significant correlation between mPRα transcript levels and the gonadosomatic index was observed during vitellogenesis and final maturation. Brain gonadotropin (FSHβ and LHβ) and mPRα were continuously expressed at different stages of ovarian development. The dynamic and diverse nature of gonadotropins and mPRα gene expression patterns suggests that these genes might jointly regulate ovarian development and maturation through the brain–pituitary–ovary axis in the rock bream endocrine system. J. Exp. Zool. 323A: 430–444, 2015. © 2015 Wiley Periodicals, Inc.
- Published
- 2015
42. Insulin-like growth factors I and II in starry flounder (Platichthys stellatus): molecular cloning and differential expression during embryonic development
- Author
-
Xuezhou Liu, Kun Zang, Xueying Shi, Cunyu Li, Yongjiang Xu, and Bao Shi
- Subjects
Gills ,Male ,DNA, Complementary ,Physiology ,Molecular Sequence Data ,Sequence Homology ,Flounder ,Aquatic Science ,Real-Time Polymerase Chain Reaction ,Biochemistry ,Open Reading Frames ,Insulin-Like Growth Factor II ,Complementary DNA ,Animals ,Cluster Analysis ,Amino Acid Sequence ,Cloning, Molecular ,Insulin-Like Growth Factor I ,Gene ,Phylogeny ,Starry flounder ,DNA Primers ,Genetics ,Analysis of Variance ,Sex Characteristics ,Messenger RNA ,Base Sequence ,biology ,Embryogenesis ,Brain ,Gene Expression Regulation, Developmental ,Sequence Analysis, DNA ,General Medicine ,biology.organism_classification ,Blastula ,Molecular biology ,Gastrulation ,Open reading frame ,Liver ,Female - Abstract
In order to elucidate the possible roles of insulin-like growth factors I and II (IGF-I and IGF-II) in the embryonic development of Platichthys stellatus, their cDNAs were isolated and their spatial expression pattern in adult organs and temporal expression pattern throughout embryonic development were examined by quantitative real-time PCR assay. The IGF-I cDNA sequence was 1,268 bp in length and contained an open reading frame (ORF) of 558 bp, which encoded 185 amino acid residues. With respect to IGF-II, the full-length cDNA was 899 bp in length and contained a 648-bp ORF, which encoded 215 amino acid residues. The amino acid sequences of IGF-I and IGF-II exhibited high identities with their fish counterparts. The highest IGF-I mRNA level was found in the liver for both sexes, whereas the IGF-II gene was most abundantly expressed in female liver and male liver, gill, and brain. The sex-specific and spatial expression patterns of IGF-I and IGF-II mRNAs are thought to be related to the sexually dimorphic growth and development of starry flounder. Both IGF-I and IGF-II mRNAs were detected in unfertilized eggs, which indicated that IGF-I and IGF-II were parentally transmitted. Nineteen embryonic development stages were tested. IGF-I mRNA level remained high from unfertilized eggs to low blastula followed by a significant decrease at early gastrula and then maintained a lower level. In contrast, IGF-II mRNA level was low from unfertilized eggs to high blastula and peaked at low blastula followed by a gradual decrease. Moreover, higher levels of IGF-I mRNA than that of IGF-II were found from unfertilized eggs to high blastula, vice versa from low blastula to newly hatched larva, and the different expression pattern verified the differential roles of IGF-I and IGF-II in starry flounder embryonic development. These results could help in understanding the endocrine mechanism involved in the early development and growth of starry flounder.
- Published
- 2014
43. Urate promotes SNCA/α-synuclein clearance via regulating mTOR-dependent macroautophagy
- Author
-
Xian Cao, Li-Fang Hu, Bao-Shi Yuan, Xiao-Ou Hou, Weifeng Luo, Yu-Lan Sheng, Qi-Lin Zhang, Xin Yuan, Xing Chen, Chun-Feng Liu, and Yu-Qing Yuan
- Subjects
0301 basic medicine ,Autophagosome ,Genetically modified mouse ,Male ,Cell Survival ,Metabolic Clearance Rate ,Mice, Transgenic ,Pilot Projects ,Biology ,PC12 Cells ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Developmental Neuroscience ,Autophagy ,Animals ,Humans ,PI3K/AKT/mTOR pathway ,Dose-Response Relationship, Drug ,Activator (genetics) ,TOR Serine-Threonine Kinases ,Wild type ,Bafilomycin ,ULK1 ,Cell biology ,Rats ,Uric Acid ,Mice, Inbred C57BL ,030104 developmental biology ,Neurology ,chemistry ,Biochemistry ,alpha-Synuclein ,030217 neurology & neurosurgery - Abstract
Serum urate levels are reported to be significantly lowered in patients with Parkinson's disease (PD) and inversely correlated to the risk and progression of PD. However, the mechanism by which urate affects PD is poorly understood. Here we showed that treatment with uric acid (UA) resulted in an autophagy activity enhancement in PC12 cells in dose- and time-dependent manners, as indicated by LC3-II increase and P62 decrease. Moreover, UA was still able to increase the LC3-II level and the number of LC3 puncta in the presence of Bafilomycin A1, a lysosomal inhibitor. These changes of autophagic markers were preceded by mTOR inhibition and ULK1 activation. Co-treatment with 3-benzyl-5-((2-nitrophenoxy) methyl)-dihydrofuran-2(3H)-one (3BDO), an mTOR activator, abolished the UA-induced LC3-II increase. More importantly, UA reduced SNCA/α-synuclein accumulation in PC12 cells that overexpress wildtype or A53T mutant SNCA, and this was blocked by Bafilomycin A1 co-treatment. The in vivo study showed that UA administration was able to modulate the levels of autophagy markers, increase the autophagosome/autolysosome formation, and reduce SNCA accumulation in the midbrain of SNCAA53T transgenic mice. Taken together, our findings suggest that UA could induce autophagy activation via an mTOR-dependent signaling and ameliorate SNCA accumulation. This implicates that urate-elevating agent may become a potential strategy for PD therapy.
- Published
- 2017
44. Targeting autophagy overcomes Enzalutamide resistance in castration-resistant prostate cancer cells and improves therapeutic response in a xenograft model
- Author
-
Yang Jinyong, Derya Tilki, Hao G. Nguyen, Primo N. Lara, Hsing Jien Kung, Allen C. Gao, R. W. Devere White, Christopher P. Evans, and Xu-Bao Shi
- Subjects
Male ,Aging ,Cancer Research ,Mice, SCID ,Pharmacology ,Castration-Resistant ,Mice ,chemistry.chemical_compound ,Prostate cancer ,0302 clinical medicine ,Cancer ,0303 health sciences ,Tumor ,Prostate Cancer ,Chloroquine ,Metformin ,3. Good health ,Gene Expression Regulation, Neoplastic ,Prostatic Neoplasms, Castration-Resistant ,5.1 Pharmaceuticals ,030220 oncology & carcinogenesis ,Benzamides ,Original Article ,Development of treatments and therapeutic interventions ,Signal Transduction ,medicine.drug ,Urologic Diseases ,Programmed cell death ,Bicalutamide ,Clinical Sciences ,Oncology and Carcinogenesis ,Biology ,SCID ,Cell Line ,03 medical and health sciences ,Cell Line, Tumor ,Nitriles ,Phenylthiohydantoin ,Autophagy ,Androgen Receptor Antagonists ,Genetics ,medicine ,Animals ,Humans ,Enzalutamide ,Oncology & Carcinogenesis ,Molecular Biology ,PI3K/AKT/mTOR pathway ,030304 developmental biology ,Neoplastic ,Prostatic Neoplasms ,AMPK ,medicine.disease ,Xenograft Model Antitumor Assays ,Androgen receptor ,Gene Expression Regulation ,chemistry ,Clomipramine - Abstract
Macro-autophagy is associated with drug resistance in various cancers and can function as an adaptive response to maintain cell survival under metabolic stresses, including androgen deprivation. Androgen deprivation or treatment with androgen receptor (AR) signaling inhibitor (ARSI), Enzalutamide (MDV-3100, ENZA) or bicalutamide induced autophagy in androgen-dependent and in castration-resistant CaP (castration-resistant prostate cancer (CRPC)) cell lines. The autophagic cascade triggered by AR blockage, correlated with the increased light chain 3-II/I ratio and ATG-5 expression. Autophagy was observed in a subpopulation of C4-2B cells that developed insensitivity to ENZA after sustained exposure in culture. Using flow cytometry and clonogenic assays, we showed that inhibiting autophagy with clomipramine (CMI), chloroquine or metformin increased apoptosis and significantly impaired cell viability. This autophagic process was mediated by AMP-dependent protein kinase (AMPK) activation and the suppression of mammalian target of rapamycin (mTOR) through Raptor phosphorylation (Serine 792). Furthermore, small interfering RNA targeting AMPK significantly inhibited autophagy and promoted cell death in CaP cells acutely or chronically exposed to ENZA or androgen deprivation, suggesting that autophagy is an important survival mechanism in CRPC. Lastly, in vivo studies with mice orthotopically implanted with ENZA-resistant cells demonstrated that the combination of ENZA and autophagy modulators, CMI or metformin significantly reduced tumor growth when compared with control groups (P
- Published
- 2014
45. Optimising pulsed microwave-vacuum puffing for Shiitake mushroom (Lentinula edodes) caps and comparison of characteristics obtained using three puffing methods
- Author
-
Xue-Dong Chang, Peng-Bao Shi, Li-Jie Liu, and Su-Wen Liu
- Subjects
Mushroom ,biology ,Central composite design ,Chemistry ,Vacuum pressure ,biology.organism_classification ,Industrial and Manufacturing Engineering ,Expansion ratio ,Lentinula ,Response surface methodology ,Food science ,Water content ,Microwave ,Food Science - Abstract
Summary Shiitake mushroom (Lentinula edodes) caps were puffed using pulsed microwave-vacuum puffing (PMVP), microwave-vacuum puffing (MVP) and microwave puffing (MP). The response surface method was used to optimise the process parameters for using PMVP to puff mushroom caps. The experiments were conducted using a central composite design to determine the visual appearance and initial moisture content (30–50%) of the mushroom caps. The initial moisture content and microwave power intensity showed highly significant linear relations with the expansion ratio and sensory evaluation. The optimal conditions for puffing mushroom caps were initial moisture content, microwave intensity, vacuum pressure and pulse ratio of 35%, 40 W g−1, 77.5 ± 1.5 kPa and 1.35, which resulted in an optimal expansion ratio and sensory score of 163.2% and 6.83, respectively. The caps puffed using PMVP showed better textures, sensory scores and microstructures than those puffed using either MP or MVP.
- Published
- 2014
46. Morphometric characteristics of the embryonic and postembryonic development of yellowtail kingfish, Serida aureovittata
- Author
-
Rong Li, Yanxiang Zhang, Bin Wang, Bao Shi, Zhengrong Zhang, Yongjiang Xu, Xuezhou Liu, and Yongshan Liu
- Subjects
Management, Monitoring, Policy and Law ,Aquatic Science ,Biology ,Embryonic stem cell ,Ecology, Evolution, Behavior and Systematics ,Cell biology - Published
- 2019
47. Complete sequence and gene organization of the mitochondrial genome of Seriola aureovittata
- Author
-
Yan Jiang, Yongshan Liu, Yanxiang Zhang, Bin Wang, Yongjiang Xu, Quanyi Gao, Bao Shi, Xuesong Song, and Xuezhou Liu
- Subjects
Genetics ,Complete sequence ,Mitochondrial DNA ,Gene Organization ,Seriola ,Management, Monitoring, Policy and Law ,Aquatic Science ,Biology ,biology.organism_classification ,Ecology, Evolution, Behavior and Systematics - Published
- 2019
48. Physiological role of the growth axis in embryonic development and early growth of Cynoglossus semilaevis
- Author
-
Xuezhou Liu, Yan Jiang, Yaxing Zhang, Bin Wang, Bao Shi, Yongjiang Xu, and Aijun Cui
- Subjects
Cynoglossus semilaevis ,Embryogenesis ,Management, Monitoring, Policy and Law ,Aquatic Science ,Biology ,Ecology, Evolution, Behavior and Systematics ,Cell biology - Published
- 2019
49. Prokaryotic expression and bioactivity analysis of insulin-like growth factor-I from Cynoglossus semilaevis Güther
- Author
-
Xuezhou Liu, Yongjiang Xu, Bao Shi, Zhiliang Liu, and Yanyan Wang
- Subjects
Insulin-like growth factor ,Biochemistry ,Cynoglossus semilaevis ,medicine.medical_treatment ,medicine ,Prokaryotic expression ,Management, Monitoring, Policy and Law ,Aquatic Science ,Biology ,Ecology, Evolution, Behavior and Systematics - Published
- 2013
50. A meta-analysis of the association between glutathione S-transferase P1 gene polymorphism and the risk of adenocarcinomas of lung cancer
- Author
-
Yan Liang, Gui-Xiong Zheng, Yi Feng, Xu Feng, Jun-Yuan Zhang, Bao-Shi Zheng, and Hong Zhong
- Subjects
Cancer Research ,Lung Neoplasms ,Polymorphism, Genetic ,biology ,Adenocarcinoma of Lung ,General Medicine ,Adenocarcinoma ,Cochrane Library ,medicine.disease ,GSTP1 ,Glutathione S-transferase ,Glutathione S-Transferase pi ,Oncology ,Risk Factors ,Meta-analysis ,Genotype ,Genetics ,medicine ,Cancer research ,biology.protein ,Humans ,Genetic Predisposition to Disease ,Gene polymorphism ,Allele ,Lung cancer - Abstract
Background Results of the published reports on the relationship between glutathione S-transferase P1 (GSTP1) gene polymorphism and the adenocarcinomas of lung cancer are still debated. Objective This meta-analysis was performed to evaluate the association between GSTP1 A/G gene polymorphism and the risk of adenocarcinomas of lung cancer. Methods The association investigations were identified from PubMed and Cochrane Library, and eligible studies were included and synthesized using meta-analysis method. Results 16 reports were included into this meta-analysis for the association of GSTP1 A/G gene polymorphism and the risk of adenocarcinomas of lung cancer. The G allele and GG genotype were not associated with the susceptibility of risk of adenocarcinomas. Furthermore, in the sensitivity analysis, the results were similar with those from the non-sensitivity analysis. Conclusions GSTP1 G allele or GG genotype is not a biomarker to be associated with the susceptibility of adenocarcinomas of lung cancer.
- Published
- 2013
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