1. Amoebicidal activity of the rhizomes and aerial parts of Allium sivasicum on Entamoeba histolytica
- Author
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Serpil Değerli, Seyda Berk, Bektas Tepe, Erdogan Malatyali, [Berk, S. -- Tepe, B.] Cumhuriyet Univ, Fac Sci & Literature, Dept Mol Biol & Genet, TR-58140 Sivas, Turkey -- [Degerli, S. -- Malatyali, E.] Cumhuriyet Univ, Sch Med, Dept Parasitol, TR-58140 Sivas, Turkey, and TEPE, Bektas -- 0000-0001-8982-5188
- Subjects
Time Factors ,Turkey ,Cell Survival ,Antiprotozoal Agents ,Allium ,Microbiology ,Entamoeba histolytica ,chemistry.chemical_compound ,Humans ,Amoebicides ,Cell survival ,Entamoebiasis ,General Veterinary ,biology ,Traditional medicine ,Allicin ,Plant Extracts ,Entamoeba ,General Medicine ,biology.organism_classification ,Rhizome ,Infectious Diseases ,chemistry ,Child, Preschool ,Insect Science ,Plant species ,Parasitology - Abstract
WOS: 000305532700005, PubMed ID: 22215196, Amebiasis is a severe illness caused by Entamoebachistolytica. The aim of this study is to evaluate the in vitro amebicidal activity of the rhizomes and aerial parts of Allium sivasicum, an endemic plant species from the flora of Turkey. Both extracts showed a time- and dose-dependent amebicidal action on the trophozoites. Among the extracts tested, rhizomes of A. sivasicum showed the strongest amebicidal effect on the trophozoites. In the presence of the rhizome extract at 2.0 mg/ml concentration, all of the trophozoites available in media have completely been killed within the 72nd hour. At 4.0 mg/ml extract concentration, all of the trophozoites available in media have completely been killed by the rhizome extract from the time of 24th hour. At 32.0 mg/ml extract concentration, 73.7% of the trophozoites were successfully killed by the extract within the first experimental hour. Aerial part extract at 4.0 mg/ml concentration completely killed the trophozoited within the 48th hour of the experimental procedure. At 8.0 mg/ml extract concentration, all of the trophozoites available in media have completely been killed by the aerial part extract from the time of 24th hour. At 32.0 mg/ml extract concentration, 67.7% of the trophozoites were successfully killed by the extract within the first experimental hour. These results suggest that the plant species evaluated here is a potential therapeutic drug for the treatment of Entamoeba infections, but it still needs to be evaluated quantitatively for determining the active phytochemicals., Research Council of Cumhuriyet University (CUBAP), Sivas-TURKEY [F-346], This study is financially supported by the Research Council of Cumhuriyet University (CUBAP), Sivas-TURKEY by the project numbered with F-346.
- Published
- 2012