1. The 3D8 single chain variable fragment protein suppresses Newcastle disease virus transmission in transgenic chickens
- Author
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Gunsup Lee, Jung-Hoon Kwon, Hwi-Cheul Lee, Shanmugam Sureshkumar, Hyeon Yang, Jin-Yong Noh, Seong-Su Yuk, Sung June Byun, Jae-Seok Woo, Sun Keun Jung, Jeom Sun Kim, Chang-Seon Song, Keon Bong Oh, and Hoonsung Choi
- Subjects
Male ,animal structures ,040301 veterinary sciences ,Transgene ,Newcastle Disease ,viruses ,Newcastle disease virus (NDV) ,chicken ,Newcastle disease virus ,Virulence ,chemical and pharmacologic phenomena ,Newcastle disease ,Virus ,0403 veterinary science ,Animals, Genetically Modified ,03 medical and health sciences ,Single-chain variable fragment ,Animals ,Poultry Diseases ,030304 developmental biology ,transgenic ,0303 health sciences ,Nuclease ,lcsh:Veterinary medicine ,General Veterinary ,biology ,04 agricultural and veterinary sciences ,General Medicine ,respiratory system ,biology.organism_classification ,3D8 ScFv ,Virology ,Specific Pathogen-Free Organisms ,embryonic structures ,biology.protein ,Nucleic acid ,Immunohistochemistry ,lcsh:SF600-1100 ,Female ,Chickens ,Single-Chain Antibodies ,Research Article - Abstract
Background The 3D8 single chain variable fragment (scFv) is a mini-antibody sequence that exhibits independent nuclease activity against all types of nucleic acids. In this research, crossing a 3D8 scFv G1 transgenic rooster with wild-type hens produced 3D8 scFv G2 transgenic chickens to evaluate suppression of viral transmission. Result The transgenic chickens were identified using genomic PCR and immunohistochemistry. To evaluate Newcastle disease virus (NDV) protection conferred by 3D8 scFv expression, transgenic, non-transgenic, and specific pathogen-free (SPF) chickens were challenged with virulent NDV by direct injection or aerosol exposure. The three groups of chickens showed no significant differences (p Conclusions Our finding suggest that the 3D8 scFv could be a useful tool to prevent chickens from spreading NDV and control virus transmission.
- Published
- 2020