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Your search keyword '"Jacky Karpouzopoulos"' showing total 3 results
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3 results on '"Jacky Karpouzopoulos"'

1. First Report on the Prevalence and Subtype Distribution of Blastocystis sp. in Edible Marine Fish and Marine Mammals: A Large Scale-Study Conducted in Atlantic Northeast and on the Coasts of Northern France

Author

Nausicaa Gantois, Angélique Lamot, Yuwalee Seesao, Colette Creusy, Luen-Luen Li, Sébastien Monchy, Sadia Benamrouz-Vanneste, Jacky Karpouzopoulos, Jean-Luc Bourgain, Célia Rault, Fabien Demaret, Martha Baydoun, Magali Chabé, Emilie Fréalle, Cécile-Marie Aliouat-Denis, Mélanie Gay, Gabriela Certad, Eric Viscogliosi, Centre d’Infection et d’Immunité de Lille - INSERM U 1019 - UMR 9017 - UMR 8204 (CIIL), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre Hospitalier Régional Universitaire [Lille] (CHRU Lille)-Centre National de la Recherche Scientifique (CNRS), Laboratoire de Boulogne sur mer, Agence nationale de sécurité sanitaire de l'alimentation, de l'environnement et du travail (ANSES), Groupement des Hôpitaux de l'Institut Catholique de Lille (GHICL), Laboratoire d’Océanologie et de Géosciences (LOG) - UMR 8187 (LOG), Centre National de la Recherche Scientifique (CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Institut national des sciences de l'Univers (INSU - CNRS), Institut Catholique de Lille (ICL), Université catholique de Lille (UCL), Groupe Mammifères Marins, Coordination Mammalogique du Nord de la France [Vimy] (CMNF), Observatoire PELAGIS UMS 3462 (PELAGIS), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), This study was supported by the Conseil Régional Hauts-de-France (Concerted Research Actions of Regional Initiative ARCir 13 ABC FISH No. 13003283), the regional competitiveness centre AQUIMER (Boulogne-sur-Mer, France), the Programme Orientations Stratégiques of the University of Lille, the Centre National de la Recherche Scientifique, the Institut Pasteur of Lille, the University Catholic of Lille, the French Agency for Food, Environmental and Occupational Health and Safety, the French Agency for Biodiversity and the Agglomeration Community of the City of La Rochelle. Y.S. was supported by a PhD fellowship from the Ministry of Science and Technology of Thailand (001/2555)., Institut national des sciences de l'Univers (INSU - CNRS)-Université du Littoral Côte d'Opale (ULCO)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Nord]), Université du Littoral Côte d'Opale (ULCO), Observatoire pour la Conservation de la Mégafaune Marine (PELAGIS), LIttoral ENvironnement et Sociétés (LIENSs), and La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-La Rochelle Université (ULR)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0301 basic medicine, Microbiology (medical), subtyping, 030231 tropical medicine, Zoology, Distribution (economics), Biology, intestinal parasites, Microbiology, [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology, molecular epidemiology, Article, law.invention, 03 medical and health sciences, 0302 clinical medicine, law, Virology, medicine, Parasite hosting, 14. Life underwater, marine mammals, lcsh:QH301-705.5, Polymerase chain reaction, edible marine fish, Genetic diversity, Blastocystis, [SDV.BA.MVSA]Life Sciences [q-bio]/Animal biology/Veterinary medicine and animal Health, Molecular epidemiology, business.industry, Zoonosis, transmission, Blastocystis sp, zoonosis, medicine.disease, biology.organism_classification, ssu rdna sequence, 030104 developmental biology, Transmission (mechanics), lcsh:Biology (General), blastocystis sp, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, real-time quantitative pcr, business
Abstract

International audience; Blastocystis is frequently identified in humans and animal hosts and exhibits a large genetic diversity with the identification of 17 subtypes (STs). Despite its zoonotic potential, its prevalence and ST distribution in edible marine fish and marine mammals remain unknown. A large-scale survey was thus conducted by screening 345 fish caught in Atlantic Northeast and 29 marine mammals stranded on the coasts of northern France for the presence of the parasite using real-time Polymerase Chain Reaction PCR. The prevalence of the parasite was about 3.5% in marine fish. These animals were mostly colonized by poikilotherm-derived isolates not identified in humans and corresponding to potential new STs, indicating that fish are natural hosts of Blastocystis. Marine fishes are also carriers of human STs and represent a likely limited source of zoonotic transmission. 13.8% of the marine mammals tested were colonized and 6 different STs were identified including 3 potential new STs. The risk of zoonotic transmission through marine mammals is insignificant due to the lack of repeated contact with humans. The present survey represents the first data regarding the prevalence and ST distribution of Blastocystis in marine fish and marine mammals and provides new insights into its genetic diversity, host range and transmission.

Published
2020
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2. Grey and harbour seals in France: distribution at sea, connectivity and trends in abundance at haulout sites

Author

Cécile Vincent, Willy Dabin, Armel Deniau, Laetitia Dupuis, Audrey Hemon, Jérôme Spitz, Simon Moss, Marie-Hélène Fremau, Jean-François Elder, Jacky Karpouzopoulos, Sami Hassani, Bernie J. McConnell, Pascal Provost, Vincent Ridoux, Stéphane Dixneuf, Mathilde Huon, Yannis Turpin, Florence Caurant, Cécile Lefeuvre, LIttoral ENvironnement et Sociétés - UMR 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), Centre d'Études Biologiques de Chizé - UMR 7372 (CEBC), Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR), Université des Antilles et de la Guyane (UAG), UMR 228 Espace-Dev, Espace pour le développement, Université des Antilles (UA)-Université de Guyane (UG)-Université de Montpellier (UM)-Université de La Réunion (UR)-Avignon Université (AU)-Université de Perpignan Via Domitia (UPVD)-Institut de Recherche pour le Développement (IRD), Laboratoire d'Etude des Mammifères Marins (LEMM), Océanopolis, Sea Mammal Research Unit, University of St Andrews [Scotland], Observatoire Avifaune de la ZPS Estuaire et Marais de la Basse Seine (Maison de l'Estuaire), Observatoire PELAGIS UMS 3462 (PELAGIS), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS), LIttoral ENvironnement et Sociétés - UMRi 7266 (LIENSs), Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Guyane (UG)-Université des Antilles (UA)-Institut de Recherche pour le Développement (IRD)-Université de Perpignan Via Domitia (UPVD)-Avignon Université (AU)-Université de La Réunion (UR)-Université de Montpellier (UM), Océanopolis [Brest], Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS), University of St Andrews. School of Biology, University of St Andrews. Sea Mammal Research Unit, University of St Andrews. Marine Alliance for Science & Technology Scotland, University of St Andrews. Scottish Oceans Institute, and Institut National de la Recherche Agronomique (INRA)-Université de La Rochelle (ULR)-Centre National de la Recherche Scientifique (CNRS)

Subjects
0106 biological sciences, Census, Range (biology), QH301 Biology, NDAS, Density, Oceanography, 010603 evolutionary biology, 01 natural sciences, Phoca, QH301, Abundance (ecology), Seasonal breeder, Telemetry, SDG 14 - Life Below Water, 14. Life underwater, OSPAR, MSFD, Relative species abundance, computer.programming_language, geography, English Channel, geography.geographical_feature_category, biology, 010604 marine biology & hydrobiology, Tracking, biology.organism_classification, Management units, Habitat, Harbour, [SDE]Environmental Sciences, computer, Channel (geography)
Abstract

International audience; Grey (Halichoerus grypus) and harbour seals (Phoca vitulina) are sympatric seal species, but they display distinct strategies of habitat use and connectivity between haulout sites. The distribution patterns and variations in relative abundance of both species were investigated along the French coast of the English Channel, at the southern limit of their range where seal numbers are increasing. Regular censuses conducted at all main haulout sites in mainland France showed significant seasonal variations at most sites, with more harbour seals counted during summer (breeding and moulting seasons), and more grey seals during summer only in the eastern English Channel. Trends in maximum haulout numbers at haulout sites showed a significant increase over the last five years, ranging from 9.7 to 30.9% per year for harbour seals, and from 5.8% (in the western English Channel) to 49.2% (in the eastern English Channel) per year for grey seals. These rates of increase in grey seal numbers are not linked to local pup production and most probably result from seal movements from the southwest British Isles and the North Sea, respectively. Aerial surveys conducted across the English Channel showed that most seal observations at sea were concentrated in the north-eastern English Channel. Telemetry showed that the 28 harbour seals tracked remained highly coastal, within a radius of 100 km from their haulout sites, and did not move to other known colonies. Grey seals moved much greater distances, reaching up to 1200 km from their capture site. More than half of the 45 grey seals tracked crossed the English Channel, especially during the breeding season, moving to known colonies in the southwest British Isles and the North Sea. Combining individual tracks and long-term surveys of the seal populations allowed a better understanding of the dynamics of these populations and their connectivity at a larger regional scale. The findings provide direct information for the management of grey and harbour seals within the frame of the Marine Strategy Framework Directive, and highlight focus areas where potential interactions between the two species should be monitored.

Published
2017
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3. Bite Injuries of Grey Seals (Halichoerus grypus) on Harbour Porpoises (Phocoena phocoena)

Author

Mutien-Marie Garigliany, Daniel Desmecht, Jean-Luc Bourgain, Jacky Karpouzopoulos, Thierry Jauniaux, Sylvain Pezeril, Jan Haelters, Pauline Loos, Freddy Coignoul, and Thibaut Bouveroux

Subjects
Male, Mitochondrial DNA, Seals, Earless, lcsh:Medicine, Positive control, Marine Biology, Phocoena, Porpoises, Biology, DNA, Mitochondrial, Polymerase Chain Reaction, Biochemistry, law.invention, DNA amplification, Predatory behavior, law, biology.animal, Blubber, Genetics, Animals, Bites and Stings, Marine Mammals, lcsh:Science, Polymerase chain reaction, DNA Primers, Seals, Multidisciplinary, Behavior, Animal, Geography, Biology and life sciences, lcsh:R, Sequence Analysis, DNA, DNA, Anatomy, biology.organism_classification, Predatory Behavior, Female, Veterinary Science, lcsh:Q, Skin lesion, Veterinary Pathology, Porpoise, Research Article
Abstract

Bite-like skin lesions on harbour porpoises (Phocoena phocoena) have been suspected to be caused by grey seals (Halichoerus grypus), and a few field observations have been reported. Bite-like skin lesions observed on stranded animals were characterized by two main components: large flaps of loose or missing skin and blubber with frayed edges and puncture lesions. Definitive demonstration of predation by a grey seal was not reported so far in those stranded animals. In this study, five stranded porpoises with bite-like skin lesions were swabbed for genetic investigations. In addition, the head of a recently dead grey seal was used to mimic bite-like skin injuries on a porpoise carcass. Subsequently, the artificial skin injuries were swabbed, along with the gum of the seal used for inflicting them (positive controls). Total DNA was extracted from the swabs and was used to retrieve a fragment of mitochondrial DNA by PCR. Primers were designed to amplify a specific stretch of mitochondrial DNA known to differ between grey seals and porpoises. The amplicon targeted was successfully amplified from the positive control and from two of the stranded porpoises, and grey seal-specific mitochondrial DNA was retrieved from all those samples. We conclude that (1) it is possible to detect grey seal DNA from dead porpoises even after several days in seawater and (2) bite-like skin lesions found on dead porpoises definitively result from grey seals attacks. The attacks are most likely linked with predation although, in a number of cases, scavenging and aggressive behaviour cannot be excluded.

Published
2014
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