1. Whole-genome sequence of the bovine blood fluke Schistosoma bovis supports interspecific hybridization with S. haematobium
- Author
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Kerstin Gravermann, Donald P. McManus, Shihab Hasan, Jens Stoye, Ana Oleaga, Sujeevi Nawaratna, Bonnie L. Webster, Malcolm K. Jones, Aidan M. Emery, Martha Zakrzewski, Mark A. Ragan, David Rollinson, Robin B. Gasser, Geoffrey N. Gobert, Hong You, Martin F. Lavin, Lutz Krause, Harald Oey, Pasi K. Korhonen, Neil D. Young, David Malagon Martínez, Oleaga, Ana [0000-0002-8019-7354], and Oleaga, Ana
- Subjects
Schistosoma Mansoni ,Host parasite interaction ,Proteome ,S haematobium ,Sequence assembly ,Genome ,Schistosoma japonicum ,Database and Informatics Methods ,Mitochondrial genome ,Disease control ,Invertebrate Genomics ,Biology (General) ,Phylogeny ,Genetics ,Schistosoma haematobium ,0303 health sciences ,education.field_of_study ,Mammalian Genomics ,030302 biochemistry & molecular biology ,Eukaryota ,Chromosome Mapping ,Genomics ,3. Good health ,Africa, Western ,Schistosoma bovis ,Schistosoma ,Trematoda ,Sequence Analysis ,Research Article ,Bioinformatics ,QH301-705.5 ,Immunology ,Population ,Biology ,Research and Analysis Methods ,Microbiology ,Amino acid sequence ,Middle East ,03 medical and health sciences ,Species Specificity ,Helminths ,Virology ,parasitic diseases ,Animals ,education ,Molecular Biology ,Gene ,030304 developmental biology ,Whole genome sequencing ,Base Sequence ,Whole Genome Sequencing ,Organisms ,Biology and Life Sciences ,Computational Biology ,DNA ,Comparative Genomics ,RC581-607 ,Genome Analysis ,Genomic Libraries ,biology.organism_classification ,Invertebrates ,Animal Genomics ,Africa ,Genome, Mitochondrial ,Hybridization, Genetic ,Cattle ,Parasitology ,Immunologic diseases. Allergy ,Sequence Alignment - Abstract
16 páginas, 1 tabla, 3 figuras., Mesenteric infection by the parasitic blood fluke Schistosoma bovis is a common veterinary problem in Africa and the Middle East and occasionally in the Mediterranean Region. The species also has the ability to form interspecific hybrids with the human parasite S. haematobium with natural hybridisation observed in West Africa, presenting possible zoonotic transmission. Additionally, this exchange of alleles between species may dramatically influence disease dynamics and parasite evolution. We have generated a 374 Mb assembly of the S. bovis genome using Illumina and PacBio-based technologies. Despite infecting different hosts and organs, the genome sequences of S. bovis and S. haematobium appeared strikingly similar with 97% sequence identity. The two species share 98% of protein-coding genes, with an average sequence identity of 97.3% at the amino acid level. Genome comparison identified large continuous parts of the genome (up to several 100 kb) showing almost 100% sequence identity between S. bovis and S. haematobium. It is unlikely that this is a result of genome conservation and provides further evidence of natural interspecific hybridization between S. bovis and S. haematobium. Our results suggest that foreign DNA obtained by interspecific hybridization was maintained in the population through multiple meiosis cycles and that hybrids were sexually reproductive, producing viable offspring. The S. bovis genome assembly forms a highly valuable resource for studying schistosome evolution and exploring genetic regions that are associated with species-specific phenotypic traits., This work was supported by grants from the QIMR Berghofer Medical Research Institute (Chenhall Estate) and the Australian Infectious Diseases Research Centre. NDY was supported by a National Health and Medical Research Council (NHMRC) CDF1, PKK by an NHMRC ECRF and DPM by an NHMRC SPRF (APP1102926). The work has been supported by NHMRC project grant APP1102322.
- Published
- 2019