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2 results on '"Maria Carolina Navarro"'

1. Effect of the biological functionalization of nanoparticles on magnetic CLEA preparation

Author

Maria Carolina Navarro, Johan Sebatian Hero, Mariana Zamora, María Inés Gómez, Cintia Mariana Romero, and María Belén Abdulhamid

Subjects
Nanoparticle, Biochemistry, Ferric Compounds, Fungal Proteins, chemistry.chemical_compound, Adsorption, Structural Biology, Enzyme Stability, Lipase, Magnetite Nanoparticles, Molecular Biology, Candida, biology, Active site, General Medicine, equipment and supplies, Enzymes, Immobilized, Enzyme structure, Cross-Linking Reagents, chemistry, Chemical engineering, Ionic strength, biology.protein, Magnetic nanoparticles, Glutaraldehyde, human activities
Abstract

Lipase immobilization using adsorption on magnetic nanoparticles, cross-linked enzyme aggregates (CLEA), and a combination of both techniques was investigated. Experimental designs were used for the optimization of the immobilization observing that the pH and ionic strength play a principal role during the lipase immobilization and its activity. For adsorption on magnetic nanoparticles and CLEA synthesis the optimal condition was pH and 100 mM. Besides, during the CLEA synthesis, glutaraldehyde concentration showed to be a significant effect on the enzyme activity. A comparison between a magnetic CLEA prepared with (Lip@mCLEA) and without (mCLEA) biological functionalized magnetic nanoparticles was made observing that the use of functionalized support showed the best performance activity. All biocatalytic systems developed gives to the enzyme thermal stability between 45 and 70 °C, being Lip@mCLEA the more stable biocatalyst. Similar behavior was observed at different pH, where both Lip@mCLEA and mCLEA showed stability at a range of pH 5 to 8. The immobilized biocatalysts showed the same affinity of the subtract that the free enzyme suggested that the enzyme structure not modified the active site. The combination of both types of immobilization show evidenced the importance of the biological functionalization of the support when magnetic CLEA is produced.

Published
2021

2. Design and characterization of immobilized biocatalyst with lipase activity onto magnetic magnesium spinel nanoparticles: A novel platform for biocatalysis

Author

Florencia Cecilia Spuches, Andrés H. Morales, Cintia Mariana Romero, María Inés Gómez, Maria Carolina Navarro, and Nora Ines Perotti

Subjects
0301 basic medicine, Nanoparticle, chemistry.chemical_element, 02 engineering and technology, engineering.material, 03 medical and health sciences, Colloid and Surface Chemistry, Enzyme Stability, Spectroscopy, Fourier Transform Infrared, Magnesium, Physical and Theoretical Chemistry, Lipase, Magnetite Nanoparticles, Candida, biology, Hydrolysis, Spinel, Temperature, Spectrometry, X-Ray Emission, Surfaces and Interfaces, General Medicine, Hydrogen-Ion Concentration, 021001 nanoscience & nanotechnology, Enzymes, Immobilized, 030104 developmental biology, chemistry, Biocatalysis, Thermogravimetry, biology.protein, engineering, Solvents, 0210 nano-technology, Biotechnology, Nuclear chemistry
Abstract

Lipases (EC 3.1.1.3) are very used industrial enzymes but presents drawbacks such as lack of stability, and poor recyclability. Most of these obstacles can be solved by lipase immobilization. The objective of this work was evaluated to magnetic magnesium spinel nanoparticles as support for lipase immobilization by covalent bound. The techniques used for nanoparticles synthesis presented advantages in the size selection of the nanoparticles obtained (60-100 nm). The immobilization of Candida rugosa lipase (CRL) was optimized. The optimal conditions were determined to be pH 3.7, enzyme concentration of 1.1 mg/mL at 4 °C and an ionic strength of 100 mM. The CRL@MgFe

Published
2018

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