1. Role of protease inhibitors and acylation stimulating protein in the adipogenesis in 3T3-L1 cells
- Author
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Maysara M. Salem, Omniya Mahmoud Abdel Hamid, Kimura Kazuhiro, Mohamed Mohamed Soliman, and Yakut Abdel-Fattah El-Senosi
- Subjects
medicine.medical_specialty ,Time Factors ,medicine.medical_treatment ,Stimulation ,Biology ,In Vitro Techniques ,3T3L-1 cells ,chemistry.chemical_compound ,Acylation stimulating protein ,Mice ,Biosynthesis ,Internal medicine ,medicine ,Pi ,Animals ,Hypoglycemic Agents ,Insulin ,Protease Inhibitors ,Protease ,Adipogenesis ,General Veterinary ,Lipid metabolism ,3T3 Cells ,Complement C3 ,Lipid Metabolism ,Endocrinology ,chemistry ,Gene Expression Regulation ,Intercellular Signaling Peptides and Proteins ,Original Article ,acylation stimulating protein - Abstract
Treatment of AIDS (HIV) and hepatitis C virus needs protease inhibitors (PI) to prevent viral replication. Uses of PI in therapy are usually associated with a decrease in body weight and dyslipidemia. Acylation stimulating protein (ASP) is a protein synthesized in adipocytes to increase triglycerides biosynthesis, for that the relation of PI and ASP to adipogenesis is tested in this work. ASP expression was increased during 3T3-L1 differentiation and reached a peak at day 8 with cell maturation. Addition of PI during adipocytes differentiation dose dependently and significantly (p < 0.5) inhibited the degree of triglycerides (TG) accumulation. Moreover, presence of ASP (450 ng/mL) in media significantly (p < 0.5) stimulated the degree of TG accumulation and there was additive stimulation for ASP when added with insulin (10 microg/mL). Finally, when ASP in different doses (Low, 16.7; Medium, 45 and High, 450 ng/mL) incubated with a dose of x150 PI, ASP partially inhibited the PI-inhibited adipogenesis and TG accumulation. The results in this study show that PI inhibit lipids accumulation and confirm role of ASP in TG biosynthesis and adipogenesis.
- Published
- 2009