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This study presents a dataset on seasonal soils sampling from September 2016 to May 2018 in the southern part of the Central Forest Reserve (SE Valdai Hills) within a catena with Endocalcaric Albic Glossic Stagnic Profondic Retisols (Cutanic, Loamic) and Albic Gleyic Histic Retisols (Cutanic, Loamic) under coniferous-deciduous forest (Tília cordáta, Pícea ábies, Ácer platanoídes) on loess-like loams underlain by carbonate moraine deposits. 152 soil samples were taken to define total concentration of 67 chemical elements (ChEs), content of three mobile fractions (exchangeable, bound within organo-mineral complexes, bound with Fe and Mn hydroxides) of 69 ChEs and content of residual fraction, including macro elements (Al, Ca, Fe, K, Mg, Mn, Na, P, Ti, S, Si), heavy metals (Ba, Co, Cr, Cu, Ni, Pb, Rb, Sr, Th, U, V, Zn), trace elements (Ag, As, B, Be, Bi, Br, Cd, Cs, Ge, Hf, Li, Mo, Nb, Pd, Sb, Sc, Se, Sn, Ta, Te, Tl,W, Zr) and rare earth elements (Ce, Er, Eu, Gd, La, Lu, Nd, Pr, Sm, Tb, Tm, Dy, Ho, Y, Yb). We measured pH-value, total organic carbon content (TOC), seven particle-size classes ( The dataset is available from Mendeley Data (http://dx.doi.org/10.17632/r29psg69z7.1, Enchilik et al., 2020) and will be further updated.