1. Exosomal miR‐3180‐3p inhibits proliferation and metastasis of non‐small cell lung cancer by downregulating FOXP4
- Author
-
Jun Zhao, Yali Liu, Huifei Zheng, Qiuyun Chen, Tengfei Chen, and Jun Chen
- Subjects
0301 basic medicine ,Pulmonary and Respiratory Medicine ,non‐small cell lung cancer ,Lung Neoplasms ,FOXP4 ,Down-Regulation ,Mice, Nude ,Exosomes ,lcsh:RC254-282 ,Metastasis ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Nude mouse ,Cell Movement ,Carcinoma, Non-Small-Cell Lung ,microRNA ,medicine ,Animals ,Humans ,Neoplasm Metastasis ,Lung cancer ,Cell Proliferation ,A549 cell ,biology ,Cell growth ,business.industry ,Forkhead Transcription Factors ,Original Articles ,General Medicine ,Transfection ,biology.organism_classification ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,Microvesicles ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Original Article ,miR‐3180‐3p ,business - Abstract
Background Non‐small cell lung cancer (NSCLC) is one of the most malignant cancers worldwide and its pathogenesis is not completely clear. In this study, we explored the functions and mechanisms of exosomes transferring miR‐3180‐3p in NSCLC progression. Methods The expression levels of miR‐3180‐3p in NSCLC tissues and paracarcinoma tissues was obtained from the GEO database (GEO: GSE53882). Exosomes derived from A549 cells were identified. Proliferation, migration and invasion were measured after treatment with exosomal miR‐3180‐3p or transfection using miR‐3180‐3p mimics. The relationship between miR‐3180‐3p and forkhead box P4 (FOXP4) was predicted using a bioinformatic tool and measured using a dual‐luciferase reporter gene assay and western blotting. Finally, a mouse xenograft model of NSCLC cells was established to verify the function of exosomal miR‐3180‐3p in vivo. Results We found that miR‐3180‐3p decreased in both NSCLC cell lines and patient tissues. Overexpression of miR‐3180‐3p or treatment with exosomal miR‐3180‐3p significantly suppressed cell proliferation and metastasis in NSCLC cell lines. Subsequently, we found miR‐3180‐3p downregulated FOXP4 protein expression levels. Furthermore, the volumes and weights of nude mouse tumors expressing exosomal miR‐3180‐3p were significantly reduced. Conclusions Exosomal miR‐3180‐3p suppresses NSCLC progression by downregulating FOXP4 expression. Key points Significant findings of the study We found that exosomal miR‐3180‐3p suppressed NSCLC progression and also identified a miR‐3180‐3p target gene. These findings provide a foundation to determine innovative therapeutic strategies. What this study adds This study contributes to research investigating exosomal containing miRNAs., We found that exosomal miR‐3180‐3p suppressed NSCLC progression and also identified a miR‐3180‐3p target gene.
- Published
- 2021