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A checklist of the grasses of India is presented, as compiled from survey of all available literature. Of the twelve subfamilies of grasses, ten are represented in India. Most subfamilies have been examined by taxonomic experts for up-to-date nomenclature. The list includes 1506 species plus infraspecific taxa and presents information on types, synonyms, distribution within India, and habit. Twelve new combinations are made, viz.Arctopoa tibetica(Munro ex Stapf) Prob. var.aristulata(Stapf) E.A. Kellogg,comb. nov.;Chimonocalamus nagalandianus(H.B. Naithani) L.G. Clark,comb. nov.;Chionachne digitata(L.f.) E.A. Kellogg,comb. nov.;Chionachne wallichiana(Nees) E.A. Kellogg,comb. nov.;Dinebra polystachyos (R. Br.) E.A. Kellogg,comb. nov.;Moorochloa eruciformis(Sm.) Veldkamp var.divaricata(Basappa & Muniv.) E.A. Kellogg,comb. nov.;Phyllostachys nigra(Lodd. ex Lindl.) Munro var.puberula(Miq.) Kailash,comb. & stat. nov.;Tzveleviochloa schmidii(Hook. f.) E.A. Kellogg,comb. nov.;Urochloa lata(Schumach.) C.E. Hubb. var.pubescens(C.E. Hubb.) E.A. Kellogg,comb. nov.;Urochloa ramosa(L.) T.Q. Nguyen var.pubescens(Basappa & Muniy.) E.A. Kellogg,comb. nov.;Urochloa semiundulata(Hochst. ex A. Rich.) Ashalatha & V.J. Nair var.intermedia(Basappa & Muniy.) E.A. Kellogg,comb. nov.

As a part of ongoing systematic and phylogenetic studies in Polygalaceae, field collections of two pairs of North American species (Polygala balduinii and P. ramosa, and P. lutea and P. rugelii) had morphologically intermediate forms and grew sympatrically, and so were suspected to be interspecific hybrids. Although hybrids among plants are often invoked in taxonomic and floristic literature based on morphologic intermediacy, they are rarely documented and substantiated using molecular tools. We found that the morphologically intermediate individuals within intermixed populations of both species pairs did indeed share all of the variable nucleotide sites in nrITS among the parent species. Likewise, using plastid sequence data (trnL-F), we determined that in both species pairs, the parentage was bidirectional. Although some DNA inheritance phenomena (e.g. incomplete lineage sorting) can result in similar polymorphic DNA sequence data, the intermediacy in both morphology and sequence data within sympatric populations is more indicative of interspecific hybridization.

Premise of the study Phylogenomic studies employing large numbers of genes, including those based on plastid genomes (plastomes), are becoming common. Nonphotosynthetic plants such as mycoheterotrophs (which rely on root-associated fungi for essential nutrients, including carbon) tend to have highly elevated rates of plastome evolution, substantial genome reduction, or both. Mycoheterotroph plastomes therefore provide excellent test cases for investigating how extreme conditions impact phylogenomic inference. Methods We used parsimony and likelihood analysis of protein-coding gene sets from published and newly completed plastomes to infer the phylogenetic placement of taxa from the 10 angiosperm families in which mycoheterotrophy evolved. Key results Despite multiple very long branches that reflect elevated substitution rates, and frequently patchy gene recovery due to genome reduction, inferred phylogenetic placements of most mycoheterotrophic lineages in DNA-based likelihood analyses are both well supported and congruent with other studies. Amino-acid-based likelihood placements are broadly consistent with DNA-based inferences, but extremely rate-elevated taxa can have unexpected placements-albeit with weak support. In contrast, parsimony analysis is strongly misled by long-branch attraction among many distantly related mycoheterotrophic monocots. Conclusions Mycoheterotrophic plastomes provide challenging cases for phylogenomic inference, as substitutional rates can be elevated and genome reduction can lead to sparse gene recovery. Nonetheless, diverse likelihood frameworks provide generally well-supported and mutually concordant phylogenetic placements of mycoheterotrophs, consistent with recent phylogenetic studies and angiosperm-wide classifications. Previous predictions of parallel photosynthesis loss within families are supported for Burmanniaceae, Ericaceae, Gentianaceae, and Orchidaceae. Burmanniaceae and Thismiaceae should not be combined as a single family in Dioscoreales.

Caamembeca (Polygalaceae) is a genus of 13 species endemic to South America. The genus is morphologically distinctive, e.g. supported by the putative morphologic synapomorphy of paired glands in a stipular position and on the rachis. However, its monophyly has not been robustly tested as only four species have been included in previous phylogenetic analyses. We present a phylogenetic analysis of 11 species based on nrITS, matK, the trnL intron, and trnL-trnF intergenic spacer. Relationships among Caamembeca species are discussed, and three new combinations are made, Caamembeca amazonensis, Caamembeca autranii, and Caamembeca formosa, providing an improved understanding of the genus.

Three enigmatic lineages of the Polygalaceae endemic to the semi-arid region of Argentina were sampled within the framework of a molecular phylogenetic study of its genera. The regions nrITS, matK, the trnL intron, the trnL-trnF intergenic spacer, and rbcL were analyzed, and the history of some morphological characters was reconstructed in the family. Results of our phylogenetic analyses and morphological study support the recognition of three monotypic genera historically treated in the genera Bredemeyera and Polygala. A new genus, Rhamphopetalum, and a new combination, R. microphyllum, are proposed together with resurrection of Hualania and Monrosia. The emergence of two species (Bredemeyera colletioides, B. microphylla) as new genera resolves the polyphyletic status of Bredemeyera. Relationships and morphological characters among the genera of Polygalaceae are discussed. Provided here are photographic plates of Hualania colletioides, Monrosia pterolopha, and Rhamphopetalum microphyllum habits, with details of floral structures, a distribution map, typifications, and taxonomic keys for the tribes of Polygalaceae and genera within the tribe Polygaleae. Fil: Mota, Michelle. Universidade Federal Da Santa Catarina. Centro de Ciencias Biológicas.; Brasil Fil: Abbott, J. Richard. The University Of Arkansas At Monticello; Estados Unidos Fil: Salas, Roberto Manuel. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Nordeste. Instituto de Botánica del Nordeste. Universidad Nacional del Nordeste. Facultad de Ciencias Agrarias. Instituto de Botánica del Nordeste; Argentina. Universidad Nacional del Nordeste. Facultad de Ciencias Exactas y Naturales y Agrimensura; Argentina Fil: Neubig, Kurt M.. Southern Illinois University Carbondale; Estados Unidos Fil: Barea Pastore, José Floriano. Universidade Federal de Santa Catarina; Brasil

The pantropical flowering plant family Annonaceae is the most species-rich family of Magnoliales. Despite long-standing interest in the systematics of Annonaceae, no authoritative classification has yet been published in the light of recent molecular phylogenetic analyses. Here, using the largest, most representative, molecular dataset compiled on Annonaceae to date, we present, for the first time, a robust family-wide phylogenetic tree and subsequent classification. We used a supermatrix of up to eight plastid markers sequenced from 193 ingroup and seven outgroup species. Some of the relationships at lower taxonomic levels are poorly resolved, but deeper nodes generally receive high support. Annonaceae comprises four major clades, which are here given the taxonomic rank of subfamily. The description of Annonoideae is amended, and three new subfamilies are described: Anaxagoreoideae, Ambavioideae and Malmeoideae. In Annonoideae, seven tribes are recognized, one of which, Duguetieae, is described as new. In Malmeoideae, seven tribes are recognized, six of which are newly described: Dendrokingstonieae, Fenerivieae, Maasieae, Malmeeae, Monocarpieae and Piptostigmateae. This new subfamilial and tribal classification is discussed against the background of previous classifications and characters to recognize subfamilies are reviewed. © 2012 The Linnean Society of London, Botanical Journal of the Linnean Society, 2012, 169, 5–40.

Wild grapes are woody climbers, found mostly in temperate regions of the northern hemisphere, comprising the genus Vitis. Despite its importance, the evolutionary history of Vitis is still contentious. Past studies have led to conflicting hypotheses about the phylogeny, speciation events, and biogeographic history of the genus. Here we investigate the evolutionary history of Vitis using data from four chloroplast spacers (trnH-psbA, trnK-rps16, trnF-nahJ, and rp132-trnL) and the nuclear gene RPB2-I, and we explore mechanisms that could have shaped the observed distribution of current species. Maximum likelihood and Bayesian analyses provided similar results, strongly supporting the presence of two subgenera and suggesting a species clustering within subgenus Vitis that mainly mirrors the disjunction between the Old and New World. Vitis vinifera subsp. syivestris was found to be sister to the Asian species while three major clades were found in the American species. A network approach confirmed the main geographic groups and highlighted different chloroplast haplotype patterns between Asian and American species. Molecular dating analysis provided the time boundaries to discuss our results. Our study shows wild grape diversification to be a continuous and complex process that concerned the Tertiary as well as the Quaternary, most likely involving both geographical and climatic forces. Local variations in extent and timing of these forces were discussed based on observed differences between groups. In the context of the Tertiary-Quaternary debate, we provide evidence in favor of the "continuous hypothesis" to explain present diversity. Finally, two directions for future research are highlighted: (i) was the earliest grape American or Asian? and (ii) are all modern grape species real? (C) 2011 Elsevier Inc. All rights reserved.

Badiera subrhombifolia is newly described from Hispaniola, where it co-occurs with B. fuertesii and B. penaea. Badiera subrhombifolia lacks the long peduncle of the former species and the scabrous leaves of the latter; it is also often distinguishable by subrhomboid, triplinerved leaves. This new species is a near endemic to high montane forests of the “south island” of Hispaniola, i.e., the Massif de la Hotte, Massif de la Selle, and Sierra de Bahoruco.

UNLABELLED PREMISE OF THE STUDY Primers were developed for a portion of the ycf1 plastid gene in magnoliid taxa to investigate the utility of ycf1 in phylogenetic analyses. • METHODS AND RESULTS Twenty-six species across six families within the magnoliid group (Canellales, Piperales, Laurales, and Magnoliales) were sampled to examine the ability to amplify ycf1. Additionally, 29 accessions of Asimina and Deeringothamnus (Annonaceae) were sequenced to assess levels of variation in ycf1 compared to matK and trnL-F. • CONCLUSIONS Results indicate that ycf1 is easily amplified and sequenced. In Annonaceae, ycf1 provides more informative phylogenetic characters than commonly used markers such as matK and trnL-F.

Pachyanthus (Melastomataceae: Miconieae) is traditionally circumscribed as a group of 20 species of Caribbean shrubs, mostly local Cuban endemics. However, the exact number of species is unknown because generic delimitations are still unclear for most genera of Miconieae. To test the monophyly of Pachyanthus, we produced a molecular phylogeny using nrITS and plastid trnS-G sequence data for 38 taxa of Miconieae in the genera Pachyanthus, Calycogonium, Tetrazygia, and Miconia. A species of Graffenrieda, a member of the sister group, Merianieae, was used as an outgroup. In the single-gene and combined analyses Pachyanthus is never recovered as a monophyletic group in any of its traditional circumscriptions. However, it is possible to distinguish a clade, Pachyanthus s.s., which is characterized by ovate to obovate petals that are conspicuously unguiculate; calyx lobes connate before anthesis, that separate into sepal-like segments; straight, ovate to oblong, yellow anthers without connective appendages or, if present, then inconspicuous, anthers dehiscing by small apical pores, and fruits with numerous seeds, 1–2 mm long, obpyramidal, with a smooth testa. The remaining species traditionally included in Pachyanthus do not have any of these features. They do not form a single monophyletic group, but instead are scattered across several clades, which also contain species of Calycogonium, Miconia, and Tetrazygia. Additional detailed molecular and morphological studies are needed in the Miconieae to ascertain whether these clades should be treated as new genera or recircumscribed as part of currently recognized genera in the Caribbean region, such as Calycogonium and Tetrazygia.

As traditionally circumscribed, the genus Polygala has been shown to be non-monophyletic, and most of the necessary generic combinations have been made for the New World species. The genus Hebecarpa, though, still needs to be addressed, as only eight species have been transferred. Even though over 65 names within Polygala are referable to Hebecarpa, preliminary study indicates that more than half the names are likely not worthy of taxonomic recognition, but careful revisionary study is necessary for fully sorting out the species and accurate synonymisation. To make the most accurate names available for modern workers, we here transfer 11 names (and associated synonyms) which we think most likely reflect discreet evolutionary lineages (species), bringing the total number of Hebecarpa species to 19. Forty-one additional names in Polygala that are attributable to Hebecarpa are enumerated but left for synonymisation or transfer after more study.

The commercial harvest of American matsutake (Tricholoma magnivelare) is a multi-million dollar industry in the Pacific Northwest region of NorthAmerica. Howtobestmanageforsustainable mushroomproductionisuncertainandconcerns remainaboutthecumulativeeffectsofpicking in the same areas year-after-year and whether raking of surface litter and mineral soil layers to find mushrooms will reduce subsequent fruiting. Here, we evaluate the effects of several mushroom harvest techniques on American matsutake production. This study was established in the Oregon Cascades in 1994 with the selection of 18 shiros of similar mushroom production. Six mushroom harvest treatments were implemented in 1995: (1) control, (2) best management practice (BMP), (3) shallow rake, litter replaced, (4) shallow rake, no replacement, (5) deep rake, litter replaced, (6) deep rake, no replacement. These treatments were pooled into three litter disturbance groups for analysis: (a) no raking of the litter, (b) litter raked with replacement, and (c) litter raked without replacement. Matsutake production on additional shiros was monitored to further compare the control and BMP treatments. Our results demonstrate that careful picking (BMP) was not detrimental to mushroom production during the initial 10 years of mushroom harvest activity. One-time treatments in which the forest floor litter layers were removed and not replaced were strongly detrimental to matsutake production and the effects have persisted for 9 years. Matsutake production was reduced to an intermediate degree by the raking with litter replacement treatments. Damage to shiros caused by repeated raking was not tested, however we expect that the effects of repeated raking would be more severe than those reported here. Negative treatment effects were particularly noticeable in years with abundant fruiting. When environmental conditions are poor for fruiting all shiros experience low production, thereby obscuring treatment effects. Within-year and year-to-year variation in fruiting is a major challenge to studies of matsutake ecology, particularly with regard to documenting treatmenteffects. Furtherstudiesspanningyearsorevendecades willlikelybeneededtoquantifyproduction,effectsofmanagementactivities,and investigate the biology of Tricholoma magnivelare. Because this study was limited to one habitat type, extension of the results to substantially different habitats types must be made with caution. However, we speculate that since the underlying biology of matsutake fruiting is similar across a wide range of habitats, careful picking should generally not hinder subsequent fruiting when other substantial disturbance to the shiro is absent. # 2006 Elsevier B.V. All rights reserved.

The vascular flora of an abandoned limestone quarry adjacent to the Kentucky River in Clark County, Kentucky, was studied from 1993 through 1999. A total of 260 species in 181 genera from 64 families were found in five diverse habitats. Fifty-seven (21.9%) were Clark County distributional records. Seventy-three (28.1%) were exotic species; 22 of these are Kentucky naturalized invasive pest plants. Taxa are classified into Equisetophyta (2), Polypodiophyta (2), Pinophyta (1), and Magnoliophyta (255). Largest families were Asteraceae (43), Poaceae (30), Fabaceae (15), Cyperaceae (13), and Rosaceae (10). Two state-listed species, Liparis loeselii and Spiranthes lucida, were present in the quarry.

A 10-year survey of vascular plants was made of Rock Creek Research Natural Area, a 77-ha deep, narrow gorge of old-growth Hemlock-Mixed Mesophytic Forest located in Laurel County, Kentucky, on the Daniel Boone National Forest. The study documented 350 specific and infraspecific taxa in 223 genera and 93 families. Thirteen are nonindigenous naturalized species. Vascular plants include 6 Lycopodiophyta, 25 Polypodiopyta, 5 Pinophyta, and 314 Magnoliophyta; 255 are annual, biennial, and perennial herbs and 95 are woody vines, shrubs, and trees. Seventeen rare and special interest species have been recorded, including 4 that are listed by the state. The floristic survey provides a baseline reference for relative abundance, species richness, plant associations, habitats, and generalized life-forms within the Rock Creek Research Natural Area.