Author: "UFRGS" / Topic: biology - Searchworks@Jio Institute Digital Library Search Results

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253 results on '"UFRGS"'

1. Globally invariant metabolism but density-diversity mismatch in springtails

Author: Anton M. Potapov, Carlos A. Guerra, Johan van den Hoogen, Anatoly Babenko, Bruno C. Bellini, Matty P. Berg, Steven L. Chown, Louis Deharveng, Ľubomír Kováč, Natalia A. Kuznetsova, Jean-François Ponge, Mikhail B. Potapov, David J. Russell, Douglas Alexandre, Juha M. Alatalo, Javier I. Arbea, Ipsa Bandyopadhyaya, Verónica Bernava, Stef Bokhorst, Thomas Bolger, Gabriela Castaño-Meneses, Matthieu Chauvat, Ting-Wen Chen, Mathilde Chomel, Aimee T. Classen, Jerome Cortet, Peter Čuchta, Ana Manuela de la Pedrosa, Susana S. D. Ferreira, Cristina Fiera, Juliane Filser, Oscar Franken, Saori Fujii, Essivi Gagnon Koudji, Meixiang Gao, Benoit Gendreau-Berthiaume, Diego F. Gomez-Pamies, Michelle Greve, I. Tanya Handa, Charlène Heiniger, Martin Holmstrup, Pablo Homet, Mari Ivask, Charlene Janion-Scheepers, Malte Jochum, Sophie Joimel, Bruna Claudia S. Jorge, Edite Jucevica, Olga Ferlian, Luís Carlos Iuñes de Oliveira Filho, Osmar Klauberg-Filho, Dilmar Baretta, Eveline J. Krab, Annely Kuu, Estevam C. A. de Lima, Dunmei Lin, Zoe Lindo, Amy Liu, Jing-Zhong Lu, María José Luciañez, Michael T. Marx, Matthew A. McCary, Maria A. Minor, Taizo Nakamori, Ilaria Negri, Raúl Ochoa-Hueso, José G. Palacios-Vargas, Melanie M. Pollierer, Pascal Querner, Natália Raschmanová, Muhammad Imtiaz Rashid, Laura J. Raymond-Léonard, Laurent Rousseau, Ruslan A. Saifutdinov, Sandrine Salmon, Emma J. Sayer, Nicole Scheunemann, Cornelia Scholz, Julia Seeber, Yulia B. Shveenkova, Sophya K. Stebaeva, Maria Sterzynska, Xin Sun, Winda I. Susanti, Anastasia A. Taskaeva, Madhav P. Thakur, Maria A. Tsiafouli, Matthew S. Turnbull, Mthokozisi N. Twala, Alexei V. Uvarov, Lisa A. Venier, Lina A. Widenfalk, Bruna R. Winck, Daniel Winkler, Donghui Wu, Zhijing Xie, Rui Yin, Douglas Zeppelini, Thomas W. Crowther, Nico Eisenhauer, Stefan Scheu, Johann-Friedrich Blumenbach Institut für Zoologie und Anthropologie, Georg-August-University = Georg-August-Universität Göttingen, A.N. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences [Moscow] (RAS), German Centre for Integrative Biodiversity Research (iDiv), Institute of Biology, Leipzig University, Leipzig, Germany, Department of Environmental Systems Science [ETH Zürich] (D-USYS), Eidgenössische Technische Hochschule - Swiss Federal Institute of Technology [Zürich] (ETH Zürich), Department of Botany and Zoology, Biosciences Center, Federal University of Rio Grande do Norte (UFRGS), Systems Ecology, Department of Ecological Science, Vrije Universiteit, Amsterdam, The Netherlands, Community and Conservation Ecology Group [Groningen], Université de Groningen, Securing Antarctica’s Environmental Future, School of Biological Sciences, Monash University, Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Department of Zoology, Institute of Biology and Ecology, Faculty of Science, Pavol Jozef Šafárik University in Košice, Košice, Slovakia, Institute of Biology and Chemistry, Moscow Pedagogical State University, Moscow, Russia, Mécanismes Adaptatifs et Evolution (MECADEV), Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS), Department of Soil Zoology, Senckenberg Society for Nature Research, Görlitz, Germany, Department of Soil Science, Centre for Agriculture and Veterinary Science, Santa Catarina State University (UDUESC Lages), Lages, SC, Brazil (UDUESC), Environmental Science Center, Qatar University, Doha, Qatar, Department of Sciences, CEPA Camargo, Astillero, Spain, Visva Bharati University, Bengal, India, Administración de Parques Nacionales, San Antonio, Argentina, School of Biology and Environmental Science - University College of Dublin, University College Dublin [Dublin] (UCD), UCD Earth Institute, University College Dublin, Unidad Multidisciplinaria de Docencia e Investigación, Facultad de Ciencias, Campus Juriquilla, Universidad Nacional Autónoma de México, Querétaro, México, Etude et Compréhension de la biodiversité (ECODIV), Université de Rouen Normandie (UNIROUEN), Normandie Université (NU)-Normandie Université (NU), Institute of Entomology [České Budějovice] (BIOLOGY CENTRE CAS), Biology Centre of the Czech Academy of Sciences (BIOLOGY CENTRE CAS), Czech Academy of Sciences [Prague] (CAS)-Czech Academy of Sciences [Prague] (CAS), FiBL France, Department of Ecology and Evolutionary Biology, Museum of Zoology, The University of Michigan Research Museums Center, Ann Arbor, Michigan 48108-2228, USA, Centre d’Ecologie Fonctionnelle et Evolutive (CEFE), Université Paul-Valéry - Montpellier 3 (UPVM)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro Montpellier, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Montpellier (UM), Departmento de Biología Zoología, Universidad Autónoma de Madrid, Madrid, Spain, Institute of Biology Bucharest, Romanian Academy, Department of General and Theoretical Ecology, University of Bremen, University of Bremen, Conservation Ecology, Groningen Institute for Evolutionary Life Sciences (GELIFES), University of Groningen, Groningen, The Netherlands, Department of Coastal Systems, Royal Netherlands Institute for Sea Research, ‘t Horntje, the Netherlands, Forestry and Forest Products Research Institute (FFPRI), Université du Québec à Montréal = University of Québec in Montréal (UQAM), Ningbo University (NBU), Université du Québec en Outaouais (UQO), Universidad Nacional de Misiones, University of Pretoria [South Africa], Geneva School of Health Sciences, HES-SO University of Applied Sciences and Arts Western Switzerland (HEdS-Ge / HES-SO), Section of Terrestrial Ecology, Department of Ecoscience, Aarhus University, Aarhus, Denmark, Instituto de Recursos Naturales y Agrobiología de Sevilla (IRNAS), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Tallinn University of Technology, Tartu College, Department of Biological Sciences, University of Cape Town, Cape Town, South Africa, Department of Entomology, Iziko Museums of South Africa, Cape Town, South Africa, Ecologie fonctionnelle et écotoxicologie des agroécosystèmes (ECOSYS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Institute of Biology, University of Latvia, Riga, Latvia, Department of Soil Science, Centre for Agriculture and Veterinary Science, Santa Catarina State University (UDESC-Lages), Lages, SC, Brazil, Department of Animal Science, Santa Catarina State University (UDESC Oeste), Chapecó, SC, Brazil, Department of Soil and Environment, Swedish University of Agricultural Sciences, Uppsala, Sweden, Climate Impacts Research Centre (CIRC), Umeå University, Estonian University of Life Sciences (EMU), Universidade Federal do Rio de Janeiro (UFRJ), Key Laboratory of the Three Gorges Reservoir Region’s Eco-Environment, Ministry of Education, Chongqing University, Chongqing, China, Department of Biology, University of Western Ontario, University of Western Ontario (UWO), Universidad Autónoma de Madrid (UAM), Johannes Gutenberg - Universität Mainz = Johannes Gutenberg University (JGU), Rice University [Houston], Wildlife and Ecology Group [New Zealand], Massey University, Graduate School of Environment and Information Sciences, Yokohama National University, Yokohama, Japan, Department of Sustainable Crop Production (DI.PRO.VE.S.), Università Cattolica del Sacro Cuore, Piacenza, Italy, University of Cadiz, Netherlands Institute of Ecology - NIOO-KNAW (NETHERLANDS), Instituto de Biología, Universidad Nacional Autónoma de México, Ciudad Universitaria México, Natural History Museum [Vienna] (NHM), University of Natural Resources and Life Sciences, Department of Integrated Biology and Biodiversity Research, Institute of Zoology (BOKU), King Abdulaziz University, Department of Biological Sciences, Université du Québec à Montréal, Lancaster Environment Centre, Lancaster University, Smithsonian Tropical Research Institute, Senckenberg Museum of Natural History Görlitz, Senckenberg – Leibniz Institution for Biodiversity and Earth System Research - Senckenberg Gesellschaft für Naturforschung, Leibniz Association-Leibniz Association, Institute for Alpine Environment, Eurac Research, Ecology, University of Innsbruck, Leopold Franzens Universität Innsbruck - University of Innsbruck, State Nature Reserve 'Privolzhskaya Lesostep', Penza, Russia, Museum and Institute of Zoology, Polish Academy of Sciences, Key Laboratory of Urban Environment and Health, Institute of Urban Environment, Chinese Academy of Sciences, Xiamen, China, Institute of Biology Komi Science Centre UB RAS, University of Bern, Aristotle University of Thessaloniki, Great Lakes Forestry Centre, Canadian Forest Service, Natural Resources Canada, Sault Ste. Marie, ON, Canada, Department of Ecology, Swedish University of Agricultural Sciences, Uppsala, Sweden, Greensway AB, Uppsala, Sweden, Institute of Wildlife Biology and Game Management, University of Natural Resources and Life Sciences, Institute of Wildlife Management and Wildlife Biology, University of Sopron, Sopron, Hungary, Key laboratory of Wetland Ecology and Environment, Northeast Institute of Geography and Agroecology, Chinese Academy of Sciences, Changchun 130102, China, Ministry of Education Key Laboratory of Vegetation Ecology, School of Life Sciences, Northeast Normal University, Changchun 130024, Jilin Provincial Key Laboratory of Animal Resource Conservation and Utilization, School of Life Sciences, Northeast Normal University, Changchun 130024, Community Department, Helmholtz Center for Environmental Research, Halle, Germany, State University of Paraiba, Institute of Integrative Biology, Centre for Biodiversity and Sustainable Land-use [University of Göttingen] (CBL), Terrestrial Ecology (TE), Conservation Ecology Group, Govers group, Ecology & Evolution, Amsterdam Sustainability Institute, Systems Ecology, Animal Ecology, Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paul-Valéry - Montpellier 3 (UPVM)-École Pratique des Hautes Études (EPHE), Unité Mixte de Recherche sur l'Ecosystème Prairial - UMR (UREP), and VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: [SDV]Life Sciences [q-bio], Soil Science, General Physics and Astronomy, 580 Plants (Botany), 000 Computer science, knowledge & systems, Markvetenskap, 590 Tiere (Zoologie), General Biochemistry, Genetics and Molecular Biology, Soil, terrestrial food web, SDG 13 - Climate Action, Humans, Animals, 000 Informatik, Wissen, Systeme, Macroecology, Arthropods, Tundra, Ecosystem, Ekologi, Multidisciplinary, Ecology, soil fertility, collembola, Biodiversity, General Chemistry, 580 Pflanzen (Botanik), Settore AGR/11 - ENTOMOLOGIA GENERALE E APPLICATA, [SDE]Environmental Sciences, 570 Life sciences, biology, 590 Animals (Zoology), 570 Biowissenschaften, Biologie
Abstract: Soil life supports the functioning and biodiversity of terrestrial ecosystems. Springtails (Collembola) are among the most abundant soil arthropods regulating soil fertility and flow of energy through above- and belowground food webs. However, the global distribution of springtail diversity and density, and how these relate to energy fluxes remains unknown. Here, using a global dataset representing 2470 sites, we estimate the total soil springtail biomass at 27.5 megatons carbon, which is threefold higher than wild terrestrial vertebrates, and record peak densities up to 2 million individuals per square meter in the tundra. Despite a 20-fold biomass difference between the tundra and the tropics, springtail energy use (community metabolism) remains similar across the latitudinal gradient, owing to the changes in temperature with latitude. Neither springtail density nor community metabolism is predicted by local species richness, which is high in the tropics, but comparably high in some temperate forests and even tundra. Changes in springtail activity may emerge from latitudinal gradients in temperature, predation and resource limitation in soil communities. Contrasting relationships of biomass, diversity and activity of springtail communities with temperature suggest that climate warming will alter fundamental soil biodiversity metrics in different directions, potentially restructuring terrestrial food webs and affecting soil functioning. The article is an outcome of the #GlobalCollembola community initiative that is voluntarily supported by researchers around the world. Data collection and analysis was supported by the Russian Science Foundation (19-74-00154 to A.P.) and by Deutsche Forschungsgemeinschaft (493345801 to A.P. and 192626868—SFB 990 to S.S.). We acknowledge support by the Open Access Publication Funds of the Göttingen University. The following funding bodies provided support for individual contributors: ARC SRIEAS Grant SR200100005 Securing Antarctica’s Environmental Future to S.L.C., Slovak Scientific Grant Agency VEGA 1/0438/22 to Ľ.K., RFBR 19-516-60002 to N.A.K., Carl Tryggers Stiftelse för Vetenskaplig Forskning and Qatar Petroleum to J.M.A., BIO 27 (2013-2014)-MAGyP and PICTO 2084 (2012)-ANPCyT to V.B., DAAD-19-10 and MSM200962001 to T.C., grant TE, PN-III-P1-1.1-TE-2019-0358 to C.F., NWO grant 821.01.015 to O.F., National Natural Sciences Foundation of China No 41471037 and 41871042 to M.G., BIO 27 (2013-2014), MAGyP; PICT 2084 (2012), FONCyT to D.F.G., NRF South African National Antarctic Programme grant 110734 to M.G., Natural Resources Canada (NRCan), EcoEnergy Innovation Initiative under the Office of Energy Research and Development, and the Natural Sciences and Engineering Research Council of Canada (NSERC) to I.T.H., L.A.V. and L.R., Independent Research Fund Denmark grant no. DFF-4002-00384 to M.H., Estonian Science Foundation G9145 to M.I., SA-France bilateral grant to C.J., SA (NRF)/Russia (RFBR) Joint Science and Technology Research Collaboration project no. 19-516-60002 (FRBR) and no. 118904 (NRF) to M.P. and C.J., European Research Council (ERC), European Union’s Horizon 2020 research and innovation programme (grant agreement no. 677232; to N.E.); iDiv, German Research Foundation (DFG–FZT 118, 202548816) to M.J. and N.E., French National Agency of Research (ANR) (JASSUR research project; ANR-12-VBDU-0011), «Ministère de l’Agriculture et de la Pêche» and «Ministère de l’Education Nationale de la Recherche et de la Technologie» (ACTA programme), «Ministère de l’Aménagement du Territoire et de l’Environnement» (Pnetox programme), EU-funded project, ECOGEN QLK5-CT-2002-01666 (www.ecogen.dk), “Agence de l’Environnement et de la Maîtrise de l'Énergie” (BIOINDICATEUR 2, BIOTECHNOSOL), ANDRA and GISFI (www.gisfi.fr) to S.J., GRR SER-BIODIV (Région Normandie, France) to MCha, ESF9258, B02 to A.K., Fundamental Research Funds for the Central Universities (grant no. 2018CDXYCH0014) to D.L., DFG 316045089 to J.L., Massey University Research Fund grant to M.A.M., DFG SCHE 376/38-2 to M.M.P., grant from the Austria Academy of Science: Heritage_2020-043_Modeling-Museum to P.Q., Slovak Scientific Grant Agency: VEGA Nos. 1/0441/03 and 1/3267/06 to N.R., Higher Education Commission of Pakistan to M.I.R., RSF 21-74-00126 to R.A.S., Austrian Federal Government and European Union (Rural Development 2014-2020) to J.S., АААА-А17-122040600025-2 to A.A.T., Brazilian Council for Scientific and Technological Development—CNPq (grant no. 152717/2016-1) to B.R.W., 309030/2018-8 to D.Z. and 305426/2018-4 to B.C.B., National Natural Science Foundation of China (31970434, 31772491) to N.N.G., Research and Innovation Support Foundation of Santa Catarina (FAPESC) (6.309/2011-6/FAPESC) and the CNPq (563251/2010-7/CNPq) to L.C.I.O.F., O.K.-F., the Latvian Council of Science Grants no. 90.108, 93.140, 96.0110, 01.0344 to E.J., CNPq for the Research Productivity Grant (305939/2018-1) to D.B., FPI-MICINN grant in the project INTERCAPA (CGL2014-56739-R) to P.H, the Natural Sciences and Engineering Research Council of Canada (NSERC) to Z.L., Ministry of Innovation and Technology of Hungary TKP2021-NKTA-43 to D.W. Authors are grateful to Penelope Greenslade for providing the literature on Australian Collembola communities. Authors are grateful to Frans Janssens for providing the global checklist of Collembola.
Published: 2023
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2. Investigation of Listeria monocytogenes, Salmonella enterica and Yersinia enterocolitica in pig carcasses in Southern Brazil

Author: Julia Helena Montes, Mariana Meneguzzi, Luis Gustavo Corbellini, Marisa Ribeiro de Itapema Cardoso, Eduardo de Freitas Costa, Arlei Coldebella, Agnes Isadora Adamatti de Souza, Jalusa Deon Kich, JALUSA DEON KICH, CNPSA, AGNES I. A. SOUZA, UFRGS, JÚLIA MONTES, MARIANA MENEGUZZI, EDUARDO DE FREITAS COSTA, UFRGS, ARLEI COLDEBELLA, CNPSA, LUIS GUSTAVO CORBELLINI, UFRGS, and MARISA RIBEIRO DE ITAPEMA CARDOSO, UFRGS.
Subjects: Serotype, Salmonella, Veterinary medicine, Population, pork, medicine.disease_cause, Antimicrobial resistance, Inspecao post-mortem, Carcaça, 03 medical and health sciences, Antibiotic resistance, Listeria monocytogenes, Resistência a antimicrobianos, Ampicillin, SF600-1100, medicine, Pork, antimicrobial resistance, education, Yersinia enterocolitica, 030304 developmental biology, 0303 health sciences, education.field_of_study, General Veterinary, biology, 030306 microbiology, Brasil, Salmonella enterica, pigs, Brasil, Região Sul, pig carcasses, biology.organism_classification, Salmonella Enteritides, Pig carcasses, Carcaça suína, Abatedouro, Pigs, Suíno, Brazil, medicine.drug
Abstract: A intensificação da produção de suínos e os avanços no controle sanitário dos rebanhos alterou de forma importante o perfil de risco do consumo de carne suína. No cenário atual, a maioria dos microrganismos causadores de lesões macroscópicas detectáveis na inspeção post mortem não são transmissíveis por alimentos, enquanto bactérias de importância como causadoras de doenças transmitidas por alimentos não causam lesões macroscópicas. No Brasil, o Ministério da Agricultura, Pecuária e Abastecimento solicitou uma opinião científica sobre a priorização de patógenos potencialmente transmitidos pela carne suína in natura. Após conduzir uma avaliação de risco qualitativa, apenas Salmonella enterica foi classificada como de alto risco para o consumidor. O presente estudo foi parte da etapa de validação da avaliação de risco e objetivou: investigar a frequência de S. enterica, Yersinia enterocolitica e Listeria. monocytogenes; e enumerar indicadores higiênico-sanitários em carcaças de suínos abatidos sob inspeção federal em frigoríficos dedicados ao abate de suínos sob sistema intensivo de criação no sul do Brasil. Além disso, o perfil de resistência a antimicrobianos dos patógenos isolados foi investigado. A superfície de um total de 378 carcaças foi amostrada por esponjas, na etapa de pré-resfriamento em três matadouros frigoríficos (A, B, C). As amostras foram investigadas quanto à presença dos três patógenos acima mencionados e quanto à enumeração de Unidades Formadoras de Colônia (log UFC.cm-1) de mesófilos aeróbios totais (MAT) e Enterobacteriaceae. As cepas isoladas de Salmonella foram testadas quanto à resistência a onze antimicrobianos pela técnica de disco difusão. As medianas de contagem de ambos os indicadores apresentaram diferença significativa (p
Published: 2020

3. Climate influences the response of community functional traits to local conditions in bromeliad invertebrate communities

Author: Fabiola Ospina Bautista, Laura Melissa Guzman, Benjamin Gilbert, Valério D. Pillar, Régis Céréghino, Ignacio M. Barberis, Bruno Corbara, Céline Leroy, Paula M. de Omena, Michael J. Richardson, Nicholas A. C. Marino, Vanderlei J. Debastiani, Merlijn Jocque, Barbara A. Richardson, Olivier Dézerald, Gustavo Q. Romero, M. Kurtis Trzcinski, Vinicius F. Farjalla, Ana Z. Gonįalves, Guillermo Montero, A. Andrew M. MacDonald, Gustavo C. O. Piccoli, Diane S. Srivastava, Pavel Kratina, Univ. of British Columbia, Simon Fraser Univ., Científicas y Técnicas, Univ. Toulouse III – Paul Sabatier, Centre for the Synthesis and Analysis of Biodiversity (CESAB-FRB), Univ. of Toronto, Univ. Federal do Rio Grande do Sul, Univ. of Campinas, Universidade Federal do Pará (UFPA), Auvergne, IRD, ECOFOG, Univ. de los Andes, Univ. de Caldas, Queen Mary Univ. of London, Universidade de São Paulo (USP), Universidade Federal do Rio de Janeiro (UFRJ), Univ. of Puerto Rico, Agrocampus Oues, Royal Belgian Inst. of Natural Sciences, University of British Columbia (UBC), Universidad Nacional de Rosario [Santa Fe], Laboratoire Ecologie Fonctionnelle et Environnement (LEFE), Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Observatoire Midi-Pyrénées (OMP), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Météo-France -Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université de Toulouse (UT), University of Toronto, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Universidade Estadual de Campinas = University of Campinas (UNICAMP), Laboratoire Microorganismes : Génome et Environnement (LMGE), Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA), Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Instituto de Informática da UFRGS (UFRGS), Universidade Federal do Estado do Rio de Janeiro (UNIRIO), Luquillo LTER, Institute for Tropical Ecosystem Studies, College of Natural Sciences, University of Puerto Rico at Rio Piedras, Écologie et santé des écosystèmes (ESE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-INSTITUT AGRO Agrocampus Ouest, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), University of Puerto Rico at Rio Piedras Campus (UPR-RP), Royal Belgian Institute of Natural Sciences (RBINS), Laboratoire Ecologie Fonctionnelle et Environnement (ECOLAB), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées, University of Campinas [Campinas] (UNICAMP), AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Observatoire Midi-Pyrénées (OMP), Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Météo France-Centre National d'Études Spatiales [Toulouse] (CNES)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Centre National de la Recherche Scientifique (CNRS), and Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-AGROCAMPUS OUEST
Subjects: 0106 biological sciences, Ecology (disciplines), climatic variation, macroinvertebrates, Local conditions, Biology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, 010603 evolutionary biology, 01 natural sciences, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, Climate variation, functional traits, Precipitation, Ecology, Evolution, Behavior and Systematics, Organism, Invertebrate, Climatic variation, Macroinvertebrates, Ecology, 010604 marine biology & hydrobiology, [SDV.BV.BOT]Life Sciences [q-bio]/Vegetal Biology/Botanics, 15. Life on land, Bromeliads, local conditions, Community composition, 13. Climate action, Benthic zone, Trait, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, bromeliads, Functional traits
Abstract: Made available in DSpace on 2022-04-28T19:29:49Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01 Functional traits determine an organism's performance in a given environment and as such determine which organisms will be found where. Species respond to local conditions, but also to larger scale gradients, such as climate. Trait ecology links these responses of species to community composition and species distributions. Yet, we often do not know which environmental gradients are most important in determining community trait composition at either local or biogeographical scales, or their interaction. Here we quantify the relative contribution of local and climatic conditions to the structure and composition of functional traits found within bromeliad invertebrate communities. We conclude that climate explains more variation in invertebrate trait composition within bromeliads than does local conditions. Importantly, climate mediated the response of traits to local conditions; for example, invertebrates with benthic life-history traits increased with bromeliad water volume only under certain precipitation regimes. Our ability to detect this and other patterns hinged on the compilation of multiple fine-grained datasets, allowing us to contrast the effect of climate versus local conditions. We suggest that, in addition to sampling communities at local scales, we need to aggregate studies that span large ranges in climate variation in order to fully understand trait filtering at local, regional and global scales. Dept of Zoology and Biodiversity Research Centre Univ. of British Columbia Dept of Biology Simon Fraser Univ. Dept of Forest and Conservation Sciences Univ. of British Columbia Facultad de Ciencias Agrarias Inst. de Investigaciones en Ciencias Agrarias de Rosario Univ. Nacional de Rosario Consejo Nacional de Investigaciones Científicas y Técnicas Laboratoire Ecologie Fonctionnelle et Environnement Univ. de Toulouse CNRS Univ. Toulouse III – Paul Sabatier Centre for the Synthesis and Analysis of Biodiversity (CESAB-FRB) Dept of Ecology and Evolutionary Biology Univ. of Toronto Dept of Ecology and Graduate Program in Ecology Univ. Federal do Rio Grande do Sul Laboratory of Multitrophic Interactions and Biodiversity Dept of Animal Biology Inst. of Biology Univ. of Campinas Inst. of Biological Sciences Univ. Federal do Pará Laboratoire Microorganismes Génome et Environnement Univ. Clermont Auvergne AMAP Univ. Montpellier CIRAD CNRS INRA IRD ECOFOG Campus Agronomique Depto de Ciencias Biológicas Univ. de los Andes Depto de Ciencias Biológicas Univ. de Caldas School of Biological and Chemical Sciences Queen Mary Univ. of London Dept of Botany Biosciences Inst. Univ. of São Paulo Depto de Ecologia Inst. de Biologia Univ. Federal do Rio de Janeiro Programa de Pós-Graduação em Ecologia Univ. Federal do Rio de Janeiro Luquillo LTER Inst. for Tropical Ecosystem Studies Univ. of Puerto Rico UMR ESE Ecology and Ecosystem Health INRA Agrocampus Oues Dept of Zoology and Botany Univ. of São Paulo State Aquatic and Terrestrial Ecology Royal Belgian Inst. of Natural Sciences
Published: 2020
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4. Defining the combined stress response in wild Arachis

Author: Ana Cristina Miranda Brasileiro, Maria Fatima Grossi-de-Sa, Mario Alfredo de Passos Saraiva, Ana Paula Zotta Mota, Bruna Vidigal, R. C. Togawa, Patricia Messenberg Guimaraes, Andressa da Cunha Quintana Martins, Thais Nicolini Oliveira, Ana Claudia Guerra de Araújo, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Embrapa Recursos Genéticos e Biotecnologia [Brasília], INCT Plant Stress Biotech, Empresa Brasileira de Pesquisa Agropecuaria (EMBRAPA), UCB Pharma SA, Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPQ), Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES), Fundacao de Apoio a Pesquisa do Distrito Federal (FAPDF), ANA PAULA ZOTTA MOTA, UFRGS, ANA CRISTINA MIRANDA BRASILEIRO, Cenargen, BRUNA VIDIGAL, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, THAIS NICOLINI OLIVEIRA, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, ANDRESSA DA CUNHA QUINTANA MARTINS, National Institute of Science and Technology-INCT PlantStress Biotech-EMBRAPA, MARIO ALFREDO DE PASSOS SARAIVA, Cenargen, ANA CLAUDIA GUERRA DE ARAUJO, Cenargen, ROBERTO COITI TOGAWA, Cenargen, MARIA FATIMA GROSSI DE SA, Cenargen, and PATRICIA MESSEMBERG GUIMARAES, Cenargen.
Subjects: 0106 biological sciences, 0301 basic medicine, Meloidogyne Arenaria, Candidate gene, Arachis, [SDV]Life Sciences [q-bio], Science, Molecular engineering in plants, Wild Arachis, 01 natural sciences, Article, Transcriptome, 03 medical and health sciences, chemistry.chemical_compound, Gene Expression Regulation, Plant, Stress, Physiological, Arabidopsis, Animals, Tylenchoidea, Transcriptomics, Biotic, Abscisic acid, Gene, Disease Resistance, Plant Diseases, 2. Zero hunger, Genetics, Abiotic component, Multidisciplinary, Abiotic, biology, fungi, food and beverages, biology.organism_classification, Droughts, 030104 developmental biology, chemistry, Meloidogyne arenaria, Medicine, Ethylene hormone, 010606 plant biology & botany
Abstract: Nematodes and drought are major constraints in tropical agriculture and often occur simultaneously. Plant responses to these stresses are complex and require crosstalk between biotic and abiotic signaling pathways. In this study, we explored the transcriptome data of wild Arachis species subjected to drought (A-metaDEG) and the root-knot nematode Meloidogyne arenaria (B-metaDEG) via meta-analysis, to identify core-stress responsive genes to each individual and concurrent stresses in these species. Transcriptome analysis of a nematode/drought bioassay (cross-stress) showed that the set of stress responsive DEGs to concurrent stress is distinct from those resulting from overlapping A- and B-metaDEGs, indicating a specialized and unique response to combined stresses in wild Arachis. Whilst individual biotic and abiotic stresses elicit hormone-responsive genes, most notably in the jasmonic and abscisic acid pathways, combined stresses seem to trigger mainly the ethylene hormone pathway. The overexpression of a cross-stress tolerance candidate gene identified here, an endochitinase-encoding gene (AsECHI) from Arachis stenosperma, reduced up to 30% of M. incognita infection and increased post-drought recovery in Arabidopsis plants submitted to both stresses. The elucidation of the network of cross-stress responsive genes in Arachis contributes to better understanding the complex regulation of biotic and abiotic responses in plants facilitating more adequate crop breeding for combined stress tolerance.
Published: 2021
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5. New World Cactaceae Plants Harbor Diverse Geminiviruses

Author: Gerardo R. Argüello-Astorga, Arvind Varsani, Michael C. Lund, Simona Kraberger, Pierre Lefeuvre, Joshua Schreck, Denis Filloux, Anthony Khalifeh, Simone G. Ribeiro, Raul Puente-Martinez, Jesús Aarón Avalos-Calleros, Andrew M. Salywon, Safaa G. Kumari, Philippe Roumagnac, Kehinde A. Oyeniran, Kara Schmidlin, Rafaela S. Fontenele, Lucas C. Majure, Amulya Bhaskara, Wendy C. Hodgson, Martin F. Wojciechowski, Matias Köhler, Darren P. Martin, Kendal Smith, Koenraad Van Doorslaer, Christian Vernière, Ilaria N. Cobb, Arizona State University [Tempe] (ASU), The Biodesign Center for Fundamental and Applied Microbiomics, Center for Evolution and Medicine, School of Life Sciences, Desert Botanical Garden, Florida Museum of Natural History [Gainesville], University of Florida [Gainesville] (UF), University of British Columbia [Vancouver], University of Texas at Dallas [Richardson] (UT Dallas), School of Behavioral and Brain Sciences, Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), Consejo Nacional de Ciencia y Tecnología [Mexico] (CONACYT), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Laboratório de Estudos em Vegetação Campestre (LEVCamp), Programa de Pós-Graduação em Botânica, Universidade Federal do Rio Grande do Sul, Porto Alegre, Brazil, School of Animal and Comparative Biomedical Sciences, University of Arizona, Arizona Cancer Center, International Center for Agricultural Research in the Dry Areas (ICARDA), Consultative Group on International Agricultural Research [CGIAR] (CGIAR), University of Cape Town, Institute of Infectious Diseases and Moleculare MedicineComputational Biolohy Group, Plant Health Institute of Montpellier (UMR PHIM), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Embrapa Recursos Genéticos e Biotecnologia [Brasília], Structural Biology Research Unit, Department of Clinical Laboratory Sciences, The molecular work was supported by a start-up grant awarded to A.V. from Arizona State University, USA., RAFAELA S. FONTENELE, ARIZONA STATE UNIVERSITY, USA, ANDREW M. SALYWON, DESERT BOTANICAL GARDEN, USA, LUCAS C. MAJURE, UNIVERSITY OF FLORIDA, USA, ILARIA N. COBB, ARIZONA STATE UNIVERSITY, USA, AMULYA BHASKARA, ARIZONA STATE UNIVERSITY, USA, JESÚS A. AVALOS-CALLEROS, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO, GERARDO R. ARGÜELLO-ASTORGA, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO, KARA SCHMIDLIN, ARIZONA STATE UNIVERSITY, USA, ANTHONY KHALIFEH, ARIZONA STATE UNIVERSITY, USA, KENDAL SMITH, ARIZONA STATE UNIVERSITY, USA, JOSHUA SCHRECK, ARIZONA STATE UNIVERSITY, USA, MICHAEL C. LUND, ARIZONA STATE UNIVERSITY, USA, MATIAS KÖHLER, UFRGS, MARTIN F. WOJCIECHOWSKI, ARIZONA STATE UNIVERSITY, USA, WENDY C. HODGSON, DESERT BOTANICAL GARDEN, USA, RAUL PUENTE-MARTINEZ, DESERT BOTANICAL GARDEN, USA, KOENRAAD VAN DOORSLAER, UNIVERSITY OF ARIZONA, USA, SAFAA KUMARI, INTERNATIONAL CENTER FOR AGRICULTURAL RESEARCH IN THE DRY AREAS (ICARDA), LEBANON, KEHINDE A. OYENIRAN, UNIVERSITY OF CAPE TOWN, SOUTH AFRICA, CHRISTIAN VERNIÈRE, CIRAD, FRANCE, DENIS FILLOUX, CIRAD, FRANCE, PHILIPPE ROUMAGNAC, CIRAD, FRANCE, PIERRE LEFEUVRE, CIRAD, FRANCE, SIMONE DA GRACA RIBEIRO, Cenargen, SIMONA P. KRABERGER, ARIZONA STATE UNIVERSITY, USA, DARREN P. MARTIN, UNIVERSITY OF CAPE TOWN, SOUTH AFRICA, ARVIND VARSANI, ARIZONA STATE UNIVERSITY, USA., University of Britsh Columbia [Vancouver], Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de Montpellier (UM)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Human Biology, and Faculty of Health Sciences
Subjects: 0106 biological sciences, 0301 basic medicine, Cactaceae, Phylogénie, [SDV]Life Sciences [q-bio], 01 natural sciences, Genome, Curtovirus, 2. Zero hunger, Diversity, biology, Phylogenetic tree, Opuntia, QR1-502, Infectious Diseases, Geminiviridae, Curly top, Cactus, Becurtovirus, food.ingredient, Pathologie végétale, Genome, Viral, Microbiology, Article, diversity, Hemiptera, 03 medical and health sciences, food, Virology, Animals, Virus classification, Géminivirus, Plant Diseases, H20 - Maladies des plantes, Virus des végétaux, biology.organism_classification, Recombination, recombination, Recombinaison, 030104 developmental biology, Evolutionary biology, 010606 plant biology & botany
Abstract: International audience; The family Cactaceae comprises a diverse group of typically succulent plants that are native to the American continent but have been introduced to nearly all other continents, predominantly for ornamental purposes. Despite their economic, cultural, and ecological importance, very little research has been conducted on the viral community that infects them. We previously identified a highly divergent geminivirus that is the first known to infect cacti. Recent research efforts in non-cultivated and asymptomatic plants have shown that the diversity of this viral family has been under-sampled. As a consequence, little is known about the effects and interactions of geminiviruses in many plants, such as cacti. With the objective to expand knowledge on the diversity of geminiviruses infecting cacti, we used previously acquired high-throughput sequencing results to search for viral sequences using BLASTx against a viral RefSeq protein database. We identified two additional sequences with similarity to geminiviruses, for which we designed abutting primers and recovered full-length genomes. From 42 cacti and five scale insects, we derived 42 complete genome sequences of a novel geminivirus species that we have tentatively named Opuntia virus 2 (OpV2) and 32 genomes of an Opuntia-infecting becurtovirus (which is a new strain of the spinach curly top Arizona virus species). Interspecies recombination analysis of the OpV2 group revealed several recombinant regions, in some cases spanning half of the genome. Phylogenetic analysis demonstrated that OpV2 is a novel geminivirus more closely related to viruses of the genus Curtovirus, which was further supported by the detection of three recombination events between curtoviruses and OpV2. Both OpV2 and Opuntia becurtoviruses were identified in mixed infections, which also included the previously characterized Opuntia virus 1. Viral quantification of the co-infected cactus plants compared with single infections did not show any clear trend in viral dynamics that might be associated with the mixed infections. Using experimental Rhizobium-mediated inoculations, we found that the initial accumulation of OpV2 is facilitated by co-infection with OpV1. This study shows that the diversity of geminiviruses that infect cacti is under-sampled and that cacti harbor diverse geminiviruses. The detection of the Opuntia becurtoviruses suggests spill-over events between viruses of cultivated species and native vegetation. The threat this poses to cacti needs to be further investigated.
Published: 2021
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6. A GWAS in Latin Americans identifies novel face shape loci, implicating VPS13B and a Denisovan introgressed region in facial variation

Author: Tábita Hünemeier, Andres Ruiz-Linares, Samuel Canizales-Quinteros, Christel Thauvin-Robinet, Betty Bonfante, Juan Camilo Chacón-Duque, Laurence Duplomb, Charlotte Montillot, Sagnik Palmal, Javier Mendoza-Revilla, Justin Cotney, Laurence Faivre, Seth M. Weinberg, Victor Acuña-Alonzo, Emma Wentworth, Philip H. Jones, Claudia Jaramillo, Caroline Costedoat, Morgane Dubied, David J. Balding, Manfred Kayser, Pierre Faux, Francisco Rothhammer, Mirsha Sánchez-Quinto, Carla Gallo, Paola Everardo-Martínez, Macarena Fuentes-Guajardo, Evie Stergiakouli, Pirro G. Hysi, Ziyi Xiong, Giovanni Poletti, Ceferino Varón-González, Rodrigo Barquera, Maria Cátira Bortolini, Laurence J. Howe, Lauriane Poloni, Rolando González-José, Valeria Villegas, Malena Hurtado, Kaustubh Adhikari, William Arias, Vanessa Granja, Lavinia Schuler-Faccini, Virginia Ramallo, John R. Shaffer, Timothy C. Cox, Tim D. Spector, Gabriel Bedoya, Hugo Villamil-Ramírez, Caio Cesar Silva de Cerqueira, Fan Liu, Binnaz Yalcin, Jorge Gómez-Valdés, Nicolas Navarro, Anthropologie bio-culturelle, Droit, Ethique et Santé (ADES), Aix Marseille Université (AMU)-EFS ALPES MEDITERRANEE-Centre National de la Recherche Scientifique (CNRS), Biogéosciences [UMR 6282] (BGS), Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL), Universidad Peruana Cayetano Heredia (UPCH), Génétique Evolutive Humaine - Human Evolutionary Genetics, Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Equipe GAD (LNC - U1231), Lipides - Nutrition - Cancer [Dijon - U1231] (LNC), Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Bourgogne (UB)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Agro Dijon, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), University of Connecticut Health Center [Farmington], Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), University College of London [London] (UCL), Erasmus University Medical Center [Rotterdam] (Erasmus MC), Universidad de Tarapaca, The Natural History Museum [London] (NHM), Universidad de Antioquia = University of Antioquia [Medellín, Colombia], National School of Anthropology and History [Mexico City, Mexico] (NSAH), Max Planck Institute for the Science of Human History (MPI-SHH), Max-Planck-Gesellschaft, Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), Instituto Nacional de Medicina Genomica, Scientific Police of São Paulo State [Ourinhos, Brazil], Universidade de São Paulo = University of São Paulo (USP), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Centro Nacional Patagónico (CENPAT), Beijing Genomics Institute [Shenzhen] (BGI), University of Chinese Academy of Sciences [Beijing] (UCAS), University of Pittsburgh (PITT), Pennsylvania Commonwealth System of Higher Education (PCSHE), University of Bristol [Bristol], King‘s College London, FHU TRANSLAD (CHU de Dijon), Centre Hospitalier Universitaire de Dijon - Hôpital François Mitterrand (CHU Dijon), University of Melbourne, University of Missouri [Kansas City] (UMKC), University of Missouri System, The Open University [Milton Keynes] (OU), Fudan University [Shanghai], Work leading to this publication was funded by grants from the National Natural Science Foundation of China (#31771393), the Scientific and Technology Committee of Shanghai Municipality (18490750300), the Ministry of Science and Technology of China (2020YFE0201600), the Shanghai Municipal Science and Technology Major Project (2017SHZDZX01) and the 111 Project (B13016), the Leverhulme Trust (F/07 134/DF), BBSRC (BB/I021213/1), the Excellence Initiative of Aix-Marseille University–A*MIDEX (a French 'Investissements d’Avenir' program), Universidad de Antioquia (CODI sostenibilidad de grupos 2013-2014 and MASO 2013-2014), Conselho Nacional de Desenvolvimento Científico e Tecnológico, Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (Apoio a Núcleos de Excelência Program), Fundação de Aperfeiçoamento de Pessoal de Nível Superior, the National Institute of Dental and Craniofacial Research (R01-DE027023, U01-DE020078, R01-DE016148, and X01-HG007821), and a Santander Research and Scholarship Award. B.B. is supported by a doctoral scholarship from Ecole Doctorale 251 Aix-Marseille Université., ANR-11-IDEX-0001,Amidex,INITIATIVE D'EXCELLENCE AIX MARSEILLE UNIVERSITE(2011), Biogéosciences [UMR 6282] [Dijon] (BGS), Centre National de la Recherche Scientifique (CNRS)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, École pratique des hautes études (EPHE), Laboratorios de Investigación y Desarrollo (LID), Unit of Human Evolutionary Genetics, Centre de génétique - Centre de référence des maladies rares, anomalies du développement et syndromes malformatifs (CHU de Dijon), Department of Genetics and Genome Sciences, University of Connecticut (UCONN), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Centre for Biodiversity and Environment Research, Department of Genetics, Evolution and Environment, Department of Genetic Identification, Departamento de Tecnología Médica, Division of Vertebrates and Anthropology, Genética Molecular (GENMOL), Molecular Genetics Laboratory, National School of Anthropology and History, Department of Archaeogenetics [Jena] (DAG), Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Forensic Science, Universidad Nacional Autónoma de México (UNAM), Unidad de Genomica de Poblaciones Aplicada a la Salud, Universidad Nacional Autónoma de México (UNAM)-Instituto Nacional de Medicina Genomica, Scientific Police of São Paulo State, Departamento de Genética e Biologia Evolutiva, Universidade de São Paulo (USP), Departamento de Genética, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS)-Instituto de Biociencias, Instituto Patagónico de Ciencias Sociales y Humanas, Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Key Laboratory of Genomic and Precision Medicine [Beijing, China], Beijing Genomics Institute [Shenzhen] (BGI)-University of Chinese Academy of Sciences [Beijing] (UCAS), Center for Craniofacial and Dental Genetics, University of Pittsburgh School of Medicine, Pennsylvania Commonwealth System of Higher Education (PCSHE)-Pennsylvania Commonwealth System of Higher Education (PCSHE), Department of Human Genetics, Department of Anthropology, Medical Research Council Integrative Epidemiology Unit, University of the West of England [Bristol] (UWE Bristol), School of Oral and Dental Sciences, Department of Twin Research & Genetic Epidemiology, King's College London, Facultad de Medicina & Instituto de Alta Investigacion, Universidad de Tarapaca-Universidade de Chile, Melbourne Integrative Genomics, School of Mathematics and Statistics, University of Melbourne-University of Melbourne, Department of Oral and Craniofacial Sciences, University of Missouri System-University of Missouri System, School of Mathematics and Statistics [Milton Keynes], Faculty of Science, Technology, Engineering and Mathematics [Milton Keynes], The Open University [Milton Keynes] (OU)-The Open University [Milton Keynes] (OU), State Key Laboratory of Genetics Engineering & MOE Key Laboratory of Contemporary Anthropology, Fudan University [Shanghai]-School of Life Sciences, Work leading to this publication was funded by grantsfrom the National Natural Science Foundation of China (#31771393), the Scientific andTechnology Committee of Shanghai Municipality (18490750300), the Ministry ofScience and Technology of China (2020YFE0201600), the Shanghai Municipal Scienceand Technology Major Project (2017SHZDZX01) and the 111 Project (B13016), theLeverhulme Trust (F/07 134/DF), BBSRC (BB/I021213/1), the Excellence Initiative ofAix-Marseille University–A*MIDEX (a French 'Investissements d’Avenir' program),Universidad de Antioquia (CODI sostenibilidad de grupos 2013-2014 and MASO 2013-2014), Conselho Nacional de Desenvolvimento Científico e Tecnológico,Fundação de Amparo à Pesquisa do Estado do Rio Grande do Sul (Apoio a Núcleos deExcelência Program), Fundação de Aperfeiçoamento de Pessoal de Nível Superior, theNational Institute of Dental and Craniofacial Research (R01-DE027023, U01-DE020078,R01-DE016148, and X01-HG007821), and a Santander Research and Scholarship Award.B.B. is supported by a doctoral scholarship from Ecole Doctorale 251 Aix-MarseilleUniversité., and Genetic Identification
Subjects: haplotype, Latin Americans, [SDV]Life Sciences [q-bio], [SHS.ANTHRO-BIO]Humanities and Social Sciences/Biological anthropology, Vesicular Transport Proteins, Genome-wide association study, Genome-wide association studies, Regulatory sequences, purl.org/becyt/ford/1 [https], Mice, 0302 clinical medicine, Native Americans, single nucleotide polymorphism, GWAS, 10. No inequality, Two-dimensional profiles, Research Articles, Mammals, 0303 health sciences, Multidisciplinary, biology, adult, article, SciAdv r-articles, Genomic regions, Hispanic or Latino, craniofacial development, VPS13B, GENÉTICA, Phenotype, American Indian, regulatory sequence, HUMAN POPULATION, Research Article, Morphology, Latin americans, Genotype, animal experiment, introgression, Introgression, Polymorphism, Single Nucleotide, Homo denisovan, 03 medical and health sciences, male, Animals, Humans, controlled study, human, Craniofacial, Facial feature, purl.org/becyt/ford/1.6 [https], Denisovan, Gene, mouse, 030304 developmental biology, [SDV.GEN]Life Sciences [q-bio]/Genetics, nonhuman, genome-wide association study, Haplotype, face, Human Genetics, Single nucleotide polymorphisms, biology.organism_classification, photography, thickness, purl.org/pe-repo/ocde/ford#3.01.02 [https], facies, DENOSIVAN HAPLOTYPE, Evolutionary biology, Anthropology, Face, 030217 neurology & neurosurgery, purl.org/pe-repo/ocde/ford#1.06.07 [https], Genome-Wide Association Study
Abstract: We carried out a genome-wide association study in Latin Americans and identified novel face morphology loci.., To characterize the genetic basis of facial features in Latin Americans, we performed a genome-wide association study (GWAS) of more than 6000 individuals using 59 landmark-based measurements from two-dimensional profile photographs and ~9,000,000 genotyped or imputed single-nucleotide polymorphisms. We detected significant association of 32 traits with at least 1 (and up to 6) of 32 different genomic regions, more than doubling the number of robustly associated face morphology loci reported until now (from 11 to 23). These GWAS hits are strongly enriched in regulatory sequences active specifically during craniofacial development. The associated region in 1p12 includes a tract of archaic adaptive introgression, with a Denisovan haplotype common in Native Americans affecting particularly lip thickness. Among the nine previously unidentified face morphology loci we identified is the VPS13B gene region, and we show that variants in this region also affect midfacial morphology in mice.
Published: 2021
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7. Supplementation with green tea and oregano extracts on productive characteristics, blood metabolites, and antioxidant status of Jersey cows during the transition period

Author: Cristiane Matté, M.F. de Paris, Caroline Peres Klein, E.F. Vizzotto, Maira Balbinotti Zanela, Sheila Cristina Bosco Stivanin, Vivian Fischer, D. Werncke, L.T. Passos, Vinícius Stone, E. F. VIZZOTTO, UFRGS, S. C. B. STIVANIN, UFRGS, M. DE PARIS, UFRGS, L. T. PASSOS, UFRGS, D. WERNCKE, UFRGS, C. P. KLEIN, UFRGS, V. STONE, UFRGS, C. MATTÉ, UFRGS, MAIRA BALBINOTTI ZANELA, CPACT, and V. FISCHER, UFRGS.
Subjects: Chá Verde, Antioxidant, Feed intake, 040301 veterinary sciences, Sangue, medicine.medical_treatment, Feed additive, Ice calving, Green tea extract, Gado Jersey, Biology, Feed conversion ratio, SF1-1100, Bovinos, Antioxidants, 0403 veterinary science, Animal science, Orégano, Milk production, Lactation, Origanum, medicine, Vaca Leiteira, Animals, Humans, Ração, Tea, Extrato Vegetal, Postpartum Period, 0402 animal and dairy science, Dairy cows, 04 agricultural and veterinary sciences, Plant extracts, 040201 dairy & animal science, Diet, Animal culture, medicine.anatomical_structure, Milk, Leite, Oxidative stress, Dietary Supplements, Chá, Animal Science and Zoology, Extratos vegetais, Cattle, Female, Somatic cell count, Postpartum period
Abstract: Plant extracts have been recognized as beneficial to human health and have been evaluated as feed additive for domestic and companion animals. This study evaluated oregano and green tea extracts fed to Jersey cows from approximately 21 d before calving to 21 d after calving on milk production, milk composition, and blood metabolites as well as investigated immunological and antioxidant attributes. Twenty-four Jersey cows with 441 ± 27 kg of BW, 3.5 ± 0.3 of body condition score (BCS), and 2.7 ± 1.8 lactations were selected at approximately 28 d before the expected parturition date and were randomly assigned to three treatments with eight cows each: without plant extracts in diet (control ? CON), addition of 10 g per day of oregano extract (OR), and addition of 5 g per day of green tea extract (GT). Feed intake, BW, BCS, blood metabolites, hemogram as well as oxidative stress biomarkers were evaluated from approximately 3 weeks prepartum to 3 weeks postpartum (transition period) while milk production and composition were evaluated during the first 3 weeks of lactation. Plant extracts did not change BW, BCS, and DM intake (DMI) throughout the transition period, but OR increased in approximately 20% total digestive nutrients and metabolizable energy intake on days 15 and 16 postpartum compared with CON. In the prepartum, OR increased in 48% platelets count compared to the CON, while GT augmented in 142% eosinophils compared with CON. Oregano extract reduced the levels of reactive species in the erythrocytes in 40% during prepartum and postpartum compared with CON, while GT reduced its levels in 24 and 29% during prepartum and postpartum, respectively, when compared with CON. In the postpartum period, OR increased in 60% the carbonylated protein content compared with CON, while GT reduced in 45% the levels of reactive species in plasma compared with CON. During the postpartum, both extracts increased in 33% the concentration of reduced glutathione when compared with CON. Moreover, GT tended to decrease feed efficiency in 11% when compared with CON; OE reduced milk pH and somatic cell count when compared with CON. In conclusion, OE and GT did not expressively affect immunological attributes in blood but reduce some oxidative stress biomarkers without compromising productive traits of Jersey cows during the transition period. Made available in DSpace on 2021-10-13T19:01:20Z (GMT). No. of bitstreams: 1 Vizzotto-2021.pdf: 650189 bytes, checksum: 7bf537d2a8aaba258df849eb653c703f (MD5) Previous issue date: 2021
Published: 2021

8. Eighteen years of Clearfield rice in Brazil: what have we learned?

Author: Luis Antonio de Avila, Carlos H. P. Mariot, André da Rosa Ulguim, Catarine Markus, José Alberto Noldin, Aldo Merotto, Enio Marchesan, Sylvio H. B. Dornelles, Dirceu Agostinetto, André Andres, Edinalvo Rabaioli Camargo, LUIS ANTONIO DE AVILA, UFPEL, ENIO MARCHESAN, EDINALVO R. CAMARGO, UFPEL, ALDO MEROTTO JR., UFRGS, ANDRÉ DA ROSA ULGUIM, UFSM, JOSÉ ALBERTO NOLDIN, EPAGRI, ANDRE ANDRES, CPACT, CARLOS H. P. MARIOT, IRGA, DIRCEU AGOSTINETTO, UFPEL, SYLVIO H. B. DORNELLES, UFSM, and CATARINE MARKUS, UFRGS.
Subjects: Oryza sativa, biology, Resistance to imidazolinone herbicides, Weed resistance, Red rice, Integrated weed management, Plant Science, Echinochloa, biology.organism_classification, Oryza, Crop, Sustainable weed management, Roguing, Agronomy, Weed, Agronomy and Crop Science, Weedy rice
Abstract: Clearfield™ (CL) rice (Oryza sativa L.) is a weedy rice (Oryza spp.; synonym = red rice) control tool that has been used in Brazil since 2003. This system includes the use of an imidazolinone (IMI)-tolerant cultivar and the application of IMI herbicides. In this review article, Brazilian weed scientists evaluate the challenges and lessons learned over 18 yr of CL use. CL system benefits include selective weedy rice control, better crop establishment during the most advantageous period of the year, and more efficient fertilizer use. In Rio Grande do Sul state, the CL system, in conjunction with other improvements, has contributed to rice grain yield gains from 5,500 kg ha−1 before 2002 to around 8,400 kg ha−1 currently. In contrast, the main problem that has arisen over this period is the rapid evolution of IMI-resistant weedy rice, caused by gene flow from CL rice cultivars. The off-label use (rate and continuous use) of IMI herbicides has contributed to the evolution of resistance in Echinochloa spp. and other weeds. IMI herbicide carryover has also affected susceptible crops grown after CL rice. Crop rotation with soybean [Glycine max (L.) Merr.] is increasing, ensuring system sustainability. The importance of minimum tillage has also become apparent. Such cultivation includes applying nonselective herbicides before sowing or just before crop emergence (at the spiking stage to eliminate as much weedy rice as possible and other weeds at an early growth stage). It also includes the use of certified seeds free of weedy rice, following label instructions for IMI herbicides, applying the herbicide PRE followed by POST, and complementary weedy rice management practices, such as roguing of surviving weedy rice plants.
Published: 2021

9. Influence of semiochemicals present in the scales of Spodoptera frugiperda on chemotactic behavior of Trichogramma pretiosum

Author: Miguel Borges, Luiza Rodrigues Redaelli, Camila Corrêa Vargas, Raul Alberto Laumann, Maria Carolina Blassioli-Moraes, Josue Sant'Ana, CAMILA CORRÊA VARGAS, UFRGS, LUIZA RODRIGUES REDAELLI, UFRGS, JOSUÉ SANT'ANA, UFRGS, MARIA CAROLINA BLASSIOLI MORAES, Cenargen, RAUL ALBERTO LAUMANN, Cenargen, and MIGUEL BORGES, Cenargen.
Subjects: Behavior, biology, Pheromone, Foraging strategies, Zoology, Hymenoptera, Chemical ecology, Spodoptera, biology.organism_classification, Fall armyworm, Trichogrammatidae, Lepidoptera genitalia, Lepidoptera, Insect Science, Y-tube olfactometer, Noctuidae, Biological control, Egg parasitoids, Ecology, Evolution, Behavior and Systematics
Abstract: Made available in DSpace on 2021-05-14T13:30:56Z (GMT). No. of bitstreams: 1 eea.13027.pdf: 520770 bytes, checksum: 6ade4738e1f1b716f248a724be25d49b (MD5) Previous issue date: 2021
Published: 2021

10. Enteric methane mitigation strategies for ruminant livestock systems in the Latin America and Caribbean region: a meta-analysis

Author: Paulo Henrique Mazza Rodrigues, Jaime Ricardo Rosero-Noguera, Fábio Luis Henrique, André Bannink, Marcos Inácio Marcondes, Isabel Cristina Molina-Botero, Laura Bibiana Gualdrón-Duarte, Medardo Antonio Díaz Céspedes, Laura Astigarraga, María Paz Tieri, Víctor Ilich Alvarado Bolovich, Alexander N. Hristov, Xiomara Gaviria-Uribe, Telma Teresinha Berchielli, Adibe Luiz Abdalla, María Fernanda Vázquez-Carrillo, Roberto Soto-Blanco, Ana Luiza Costa Cruz Borges, F.A.S. Silva, Olga Lucía Mayorga Mogollón, Mohammed Benaouda, José Gere, L. S. Sakamoto, Thierry Ribeiro Tomich, Sandra Lucía Posada-Ochoa, Alda Lúcia Gomes Monteiro, Jacobo Arango, Luiz Gustavo Ribeiro Pereira, María Esperanza Cerón-Cucchi, Lucia Galvão de Albuquerque, Luiza Ilha Borges, Patrícia Perondi Anchão Oliveira, Octavio Alonso Castelán-Ortega, Sebastiao de Campos Valadares-Filho, Rafael Jiménez-Ocampo, Andrea Milena Sierra-Alarcón, Alexandre Berndt, Ricardo Reis e Silva, Sergio Abarca-Monge, Adibe Luiz Abdalla-Filho, Luis Alfonso Giraldo Valderrama, Ana Cláudia Ruggieri, Lorena Inés Mestra-Vargas, Carlos Alfredo Gómez-Bravo, Paulo César de Faccio Carvalho, Maria Eugênia Zerlotti Mercadante, Gustavo Jaurena, Abmael da Silva Cardoso, Rogério Martins Maurício, Juan Carlos Ku-Vera, Guilhermo Francklin de Souza Congio, Fernanda Samarini Machado, Ricardo Andrade Reis, Diego Zanetti, M. B. Chiavegato, María Paula Juliarena, Patricia Ricci, Sila Carneiro da Silva, Jusiane Rossetto, Ever del Jesus Flores-Santiago, Mariana Magalhães Campos, Flávio Perna Júnior, Tainá Silvestre Moreira, Claudia Janeth Ariza-Nieto, Jean Victor Savian, Helena Ferreira Lage, Juliana Duarte Messana, Banira Lombardi, Camila Muñoz, Rolando Barahona-Rosales, Henrique Mendonça Nunes Ribeiro-Filho, Olegario Hernández, Abimael Ortiz-Chura, João Paulo Pacheco Rodrigues, Horacio Leandro Gonda, Tibaitatá, Universidade de São Paulo (USP), Wageningen University & Research, The Pennsylvania State University, University of Buenos Aires (UBA), Swedish University of Agricultural Sciences, National Technological University (UTN), National Scientific and Technical Research Council (CONICET), National Institute of Agricultural Technology (INTA) (INTA–CONICET), Dairy Value Chain Research Institute (IDICAL) (INTA–CONICET), UTN, INTA, National University of the Centre of the Buenos Aires Province (UNCPBA), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Associated Colleges of Uberaba (FAZU), Universidade Federal do Paraná (UFPR), Santa Catarina State University (UDESC), Universidade Federal de Viçosa (UFV), São Paulo Agribusiness Technology Agency (APTA), Universidade Estadual Paulista (Unesp), Federal University of Rio Grande do Sul (UFRGS), National Institute of Agricultural Research (INIA) Treinta y Tres, Universidade Federal de Sergipe (UFS), Federal University of Southern and Southeastern Pará (UNIFESSPA), Universidade Federal de Minas Gerais (UFMG), Newton Paiva University, The Ohio State University, Science and Technology of Southern Minas Gerais, INIA Remehue, Turipaná, National Agrarian University La Molina (UNALM), Universidad Nacional de Colombia (UNAL), International Center for Tropical Agriculture (CIAT), University of Antioquia (UdeA), National Institute of Innovation and Agricultural Technology Transfer (INTA), University of Yucatan (UADY), Experimental Field Valle del Guadiana, South-Southeast Regional Unit (URUSSE), Autonomous University of the State of Mexico (UAEMex), and Environment (INRAE), AgroSup Dijon, University of the Republic of Uruguay (UdelaR), GUILHERMO FRANCKLIN DE SOUZA CONGIO, USP, ANDRÉ BANNINK, Wageningen University & Research, OLGA LUCÍA MAYORGA MOGOLLÓN, AGROSAVIA, GUSTAVO JAURENA, Universidade de Buenos Aires, HORACIO GONDA, Swedish University of Agricultural Sciences, JOSÉ IGNACIO GERE, National Technological University, Argentina / National Scientific and Technical Research Council, MARÍA ESPERANZA CERÓN-CUCCHI, National Institute of Agricultural Technology, ABIMAEL ORTIZ-CHURA, National Institute of Agricultural Technology, MARÍA PAZ TIERI, Dairy Value Chain Research Institute / National Technological University, Argentina, OLEGARIO HERNÁNDEZ, INTA, PATRICIA RICCI, National Scientific and Technical Research Council / INTA, MARÍA PAULA JULIARENA, National Scientific and Technical Research Council / National University of the Centre of the Buenos Aires Province, BANIRA LOMBARDI, National Scientific and Technical Research Council / National University of the Centre of the Buenos Aires Province, ADIBE LUIZ ABDALLA, USP, ADIBE LUIZ ABDALLA-FILHO, USP, ALEXANDRE BERNDT, CPPSE, PATRÍCIA PERONDI ANCHÃO OLIVEIRA, CPPSE, FÁBIO LUIS HENRIQUE, FAZU, ALDA LÚCIA GOMES MONTEIRO, UFPR, LUIZA ILHA BORGES, UFPR, HENRIQUE MENDONÇA NUNES RIBEIRO-FILHO, UDESC, LUIZ GUSTAVO RIBEIRO PEREIRA, CNPGL, THIERRY RIBEIRO TOMICH, CNPGL, MARIANA MAGALHÃES CAMPOS, CNPGL, FERNANDA SAMARINI MACHADO, CNPGL, MARCOS INÁCIO MARCONDES, UFV, MARIA EUGÊNIA ZERLOTTI MERCADANTE, APTA, LEANDRO SANNOMIYA SAKAMOTO, APTA, LUCIA GALVÃO ALBUQUERQUE, UNESP, PAULO CÉSAR DE FACCIO CARVALHO, UFRGS, JUSIANE ROSSETTO, UFRGS, JEAN VÍCTOR SAVIAN, UFRGS / Instituto Nacional de Pesquisa Agropecuária do Uruguai, PAULO HENRIQUE MAZZA RODRIGUES, USP, FLÁVIO PERNA JÚNIOR, USP, TAINÁ SILVESTRE MOREIRA, Universidade Estadual da Pensilvânia / USP, ROGÉRIO MARTINS MAURÍCIO, UFSJ, JOÃO PAULO PACHECO RODRIGUES, UNIFESSPA, ANA LUIZA DA COSTA CRUZ BORGES, UFMG, RICARDO REIS E SILVA, UFMG, HELENA FERREIRA LAGE, Universidade Newton Paiva, RICARDO ANDRADE REIS, UNESP, ANA CLÁUDIA RUGGIERI, UNESP, ABMAEL DA SILVA CARDOSO, UNESP, SILA CARNEIRO DA SILVA, USP, MARÍLIA BARBOSA CHIAVEGATO, Universidade Estadual de Ohio, SEBASTIÃO DE CAMPOS VALADARES-FILHO, UFV, FLÁVIA ADRIANE DE SALES SILVA, UFV, DIEGO ZANETTI, IFSULDEMINAS, TELMA TERESINHA BERCHIELLI, UNESP, JULIANA DUARTE MESSANA, UNESP, CAMILA MUÑOZ, INIA Remehue, CLAUDIA JANETH ARIZA-NIETO, AGROSAVIA, ANDREA MILENA SIERRA-ALARCÓN, AGROSAVIA, LAURA BIBIANA GUALDRÓN-DUARTE, AGROSAVIA, LORENA INÉS MESTRA-VARGAS, AGROSAVIA, ISABEL CRISTINA MOLINA-BOTERO, Universidade Nacional Agrária La Molina, ROLANDO BARAHONA-ROSALES, Universidade Nacional da Colômbia, JACOBO ARANGO, Centro Internacional de Agricultura Tropical (CIAT), XIOMARA GAVIRIA-URIBE, Universidade Nacional da Colombia / Centro Internacional de Agricultura Tropical (CIAT), LUIS ALFONSO GIRALDO VALDERRAMA, Universidade Nacional da Colombia, JAIME RICARDO ROSERO-NOGUERA, Universidade de Antioquia, SANDRA LUCÍA POSADA-OCHOA, Universidade de Antioquia, SERGIO ABARCA-MONGE, INTA, ROBERTO SOTO-BLANCO, INTA, JUAN CARLOS KU-VERA, Universidade de Yucatán, RAFAEL JIMÉNEZ-OCAMPO, Universidade de Yucatán / Instituto Nacional de Investigação Florestal, Agrícola e Pecuária (INIFAP), EVER DEL JESUS FLORES-SANTIAGO, Universidade de Yucatán / Universidade Autónoma de Chapingo, OCTAVIO ALONSO CASTELÁN-ORTEGA, Universidade Autónoma do Estado do México, MARÍA FERNANDA VÁZQUEZ-CARRILLO, Universidade Autónoma do Estado do México, MOHAMMED BENAOUDA, French National Research Institute for Agriculture, Food, and Environment (INRAE) / AgroSup Dijon, CARLOS ALFREDO GÓMEZ-BRAVO, Universidade Nacional Agrária La Molina, VÍCTOR ILICH ALVARADO BOLOVICH, Universidade Nacional Agrária La Molina, MEDARDO ANTONIO DÍAZ CÉSPEDES, Universidade Nacional Agrária La Molina, LAURA ASTIGARRAGA, Universidade da República do Uruguai, and ALEXANDER NIKOLOV HRISTOV, Universidade Estadual da Pensilvânia.
Subjects: Mitigação, Animal breeding, Animal Nutrition, Mitigation, 020209 energy, Strategy and Management, SHEPP, Efeito Estufa, Enteric methane, Context (language use), 02 engineering and technology, Beef cattle, Biology, Industrial and Manufacturing Engineering, Animal science, Ruminant, Grazing, Greenhouse gas emissions, 0202 electrical engineering, electronic engineering, information engineering, Metano entérico, Aquecimento global, Dairy cattle, 0505 law, General Environmental Science, Bovino, Sheep, Emissões de gases com efeito de estufa, Ovelha, Renewable Energy, Sustainability and the Environment, business.industry, Global warming, 05 social sciences, Building and Construction, biology.organism_classification, Diervoeding, Metano, Breed, purl.org/becyt/ford/4.2 [https], 050501 criminology, WIAS, Livestock, Cattle, business, Methane, OVINOS, purl.org/becyt/ford/4 [https]
Abstract: Latin America and Caribbean (LAC) is a developing region characterized for its importance for global food security, producing 23 and 11% of the global beef and milk production, respectively. The region's ruminant livestock sector however, is under scrutiny on environmental grounds due to its large contribution to enteric methane (CH4) emissions and influence on global climate change. Thus, the identification of effective CH4 mitigation strategies which do not compromise animal performance is urgently needed, especially in context of the Sustainable Development Goals (SDG) defined in the Paris Agreement of the United Nations. Therefore, the objectives of the current study were to: 1) collate a database of individual sheep, beef and dairy cattle records from enteric CH4 emission studies conducted in the LAC region, and 2) perform a meta-analysis to identify feasible enteric CH4 mitigation strategies, which do not compromise animal performance. After outlier's removal, 2745 animal records (65% of the original data) from 103 studies were retained (from 2011 to 2021) in the LAC database. Potential mitigation strategies were classified into three main categories (i.e., animal breeding, dietary, and rumen manipulation) and up to three subcategories, totaling 34 evaluated strategies. A random effects model weighted by inverse variance was used (Comprehensive Meta-Analysis V3.3.070). Six strategies decreased at least one enteric CH4 metric and simultaneously increased milk yield (MY; dairy cattle) or average daily gain (ADG; beef cattle and sheep). The breed composition F1 Holstein × Gyr decreased CH4 emission per MY (CH4IMilk) while increasing MY by 99%. Adequate strategies of grazing management under continuous and rotational stocking decreased CH4 emission per ADG (CH4IGain) by 22 and 35%, while increasing ADG by 22 and 71%, respectively. Increased dietary protein concentration, and increased concentrate level through cottonseed meal inclusion, decreased CH4IMilk and CH4IGain by 10 and 20% and increased MY and ADG by 12 and 31%, respectively. Lastly, increased feeding level decreased CH4IGain by 37%, while increasing ADG by 171%. The identified effective mitigation strategies can be adopted by livestock producers according to their specific needs and aid LAC countries in achieving SDG as defined in the Paris Agreement. Fil: Congio, Guilhermo Francklin de Souza. Universidade do Sao Paulo. Escola Superior de Agricultura Luiz de Queiroz; Brasil. Corporación Colombiana de Investigación Agropecuaria; Colombia Fil: Bannink, André. University of Agriculture Wageningen; Países Bajos Fil: Mayorga Mogollón, Olga Lucía. Corporación Colombiana de Investigación Agropecuaria; Colombia Fil: Jaurena, Gustavo. Universidad de Buenos Aires. Facultad de Agronomia. Departamento de Producción Animal. Cátedra de Nutrición Animal; Argentina Fil: Gonda, Horacio Leandro. Uppsala Universitet; Suecia Fil: Gere, José Ignacio. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología Veterinaria - Consejo Nacional de Investigaciones Científicas y Técnicas. Oficina de Coordinación Administrativa Parque Centenario. Instituto de Patobiología Veterinaria; Argentina Fil: Cerón Cucchi, María Esperanza. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina Fil: Ortiz Chura, Abimael. Instituto Nacional de Tecnología Agropecuaria. Centro de Investigación en Ciencias Veterinarias y Agronómicas. Instituto de Patobiología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Tieri, María Paz. Universidad Tecnológica Nacional. Facultad Regional Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Investigación de la Cadena Láctea. - Instituto Nacional de Tecnología Agropecuaria. Centro Regional Santa Fe. Estación Experimental Agropecuaria Rafaela. Instituto de Investigación de la Cadena Láctea; Argentina Fil: Hernandez, Olegario. Instituto Nacional de Tecnología Agropecuaria. Centro Regional Tucuman-Santiago del Estero; Argentina Fil: Ricci, Patricia. Instituto Nacional de Tecnologia Agropecuaria. Centro Regional Buenos Aires Sur. Estacion Experimental Agropecuaria Balcarce. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. Grupo Vinculado Estacion Experimental Agropecuaria Cuenca del Salado Al Ipads | Consejo Nacional de Investigaciones Cientificas y Tecnicas. Centro Cientifico Tecnologico Conicet - Mar del Plata. Instituto de Innovación Para la Producción Agropecuaria y El Desarrollo Sostenible. Grupo Vinculado Estacion Experimental Agropecuaria Cuenca del Salado Al Ipads.; Argentina Fil: Juliarena, María Paula. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina Fil: Lombardi, Banira. Universidad Nacional del Centro de la Provincia de Buenos Aires. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tandil. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires. - Provincia de Buenos Aires. Gobernación. Comisión de Investigaciones Científicas. Centro de Investigaciones en Física e Ingeniería del Centro de la Provincia de Buenos Aires; Argentina Fil: Abdalla, Adibe Luiz. Universidade de Sao Paulo; Brasil Fil: Abdalla Filho, Adibe Luiz. Universidade de Sao Paulo; Brasil Fil: Berndt, Alexandre. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Oliveira, Patrícia Perondi Anchão. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Henrique, Fábio Luis. Colegios Asociados de Uberaba; Brasil Fil: Monteiro, Alda Lúcia Gomes. Universidade Federal do Paraná; Brasil Fil: Borges, Luiza Ilha. Universidade Federal do Paraná; Brasil Fil: Ribeiro Filho, Henrique Mendonça Nunes. Universidade Federal de Santa Catarina; Brasil Fil: Ribeiro Pereira, Luiz Gustavo. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Tomich, Thierry Ribeiro. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Campos, Mariana Magalhães. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Machado, Fernanda Samarini. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Marcondes, Marcos Inácio. Universidade Federal de Viçosa.; Brasil Fil: Mercadante, Maria Eugênia Zerlotti. Agencia de Tecnología Agroindustrial de Sao Paulo; Argentina Fil: Sakamoto, Leandro Sannomiya. Agencia de Tecnología Agroindustrial de Sao Paulo; Argentina Fil: Albuquerque, Lucia Galvão. Universidade Estadual Paulista Julio de Mesquita Filho; Brasil Fil: Carvalho, Paulo César de Faccio. Universidade Federal do Rio Grande do Sul; Brasil Fil: Hristov, Alexander Nikolov. State University of Pennsylvania; Estados Unidos. University of Agriculture Wageningen; Países Bajos. Universidade de Sao Paulo; Brasil. Corporación Colombiana de Investigación Agropecuaria; Colombia
Published: 2021

11. RNAi-Mediated Suppression of Laccase2 Impairs Cuticle Tanning and Molting in the Cotton Boll Weevil (Anthonomus grandis)

Author: Isabela Tristan Lourenço-Tessutti, Diogo Martins-de-Sa, Marilia Santos Silva, Janice de Almeida Engler, Walter R. Terra, Daniele H. Pinheiro, Fabrício Barbosa Monteiro Arraes, Roberta Ramos Coelho, José Dijair Antonino, Maria Cristina Mattar da Silva, Maria Fatima Grossi-de-Sa, Alexandre Augusto Pereira Firmino, Clidia Eduarda Moreira-Pinto, Fernando Campos de Assis Fonseca, Leonardo Lima Pepino de Macedo, Embrapa Recursos Genéticos e Biotecnologia [Brasília], Max Planck Institute of Molecular Plant Physiology (MPI-MP), Max-Planck-Gesellschaft, University of Brasilia [Brazil] (UnB), Universidade Federal Rural de Pernambuco (UFRPE), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), National Institute of Science and Technology (INCT), Brazilian Agricultural Research Corporation (Embrapa), Institut Sophia Agrobiotech (ISA), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Université Côte d'Azur (UCA), Département Santé des Plantes et Environnement (DPT SPE), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), University of São Paulo (USP), Universidade Católica de Brasília (UCB), Associação Brasileira dos Produtores de Algodão, INCT – Plant Stress Biotech (Grant Numbers 193.001.265/2017 and 465480/2014-4, ALEXANDRE AUGUSTO PEREIRA FIRMINO, DANIELE HELOÍSA PINHEIRO, CLIDIA EDUARDA MOREIRA-PINTO, UNB, JOSÉ DIJAIR ANTONINO, UFRPE, LEONARDO LIMA PEPINO DE MACEDO, Cenargen, DIOGO MARTINS-DE-SA, UNB, FABRÍCIO BARBOSA MONTEIRO ARRAES, UFRGS, ROBERTA RAMOS COELHO, FERNANDO CAMPOS DE ASSIS FONSECA, UNB, MARIA CRISTINA MATTAR DA SILVA, Cenargen, JANICE DE ALMEIDA ENGLER, INCT PLANT STRESS BIOTECH, MARILIA SANTOS SILVA, Cenargen, ISABELA TRISTAN LOURENCO TESSUTTI, Cenargen, WALTER RIBEIRO TERRA, USP, and MARIA FATIMA GROSSI DE SA, Cenargen.
Subjects: 0106 biological sciences, 0301 basic medicine, Integrated pest management, Physiology, media_common.quotation_subject, Cuticle, Insect pest control, Laccase2, Insect, Biology, 01 natural sciences, lcsh:Physiology, gene silencing, 03 medical and health sciences, RNA interference, Physiology (medical), Botany, media_common, 2. Zero hunger, Larva, Cuticle tanning, lcsh:QP1-981, [SDV.BA]Life Sciences [q-bio]/Animal biology, fungi, Gene silencing, biology.organism_classification, [SDV.BV.PEP]Life Sciences [q-bio]/Vegetal Biology/Phytopathology and phytopharmacy, Pupa, cuticle tanning, 010602 entomology, 030104 developmental biology, CONTROLE DE PRAGAS, insect pest control, Anthonomus, RNAi, PEST analysis
Abstract: International audience; The cotton boll weevil, Anthonomus grandis, is the most economically important pest of cotton in Brazil. Pest management programs focused on A. grandis are based mostly on the use of chemical insecticides, which may cause serious ecological impacts. Furthermore, A. grandis has developed resistance to some insecticides after their long-term use. Therefore, alternative control approaches that are more sustainable and have reduced environmental impacts are highly desirable to protect cotton crops from this destructive pest. RNA interference (RNAi) is a valuable reverse genetics tool for the investigation of gene function and has been explored for the development of strategies to control agricultural insect pests. This study aimed to evaluate the biological role of the Laccase2 (AgraLac2) gene in A. grandis and its potential as an RNAi target for the control of this insect pest. We found that AgraLac2 is expressed throughout the development of A. grandis with significantly higher expression in pupal and adult developmental stages. In addition, the immunolocalization of the AgraLac2 protein in third-instar larvae using specific antibodies revealed that AgraLac2 is distributed throughout the epithelial tissue, the cuticle and the tracheal system. We also verified that the knockdown of AgraLac2 in A. grandis resulted in an altered cuticle tanning process, molting defects and arrested development. Remarkably, insects injected with dsAgraLac2 exhibited defects in cuticle hardening and pigmentation. As a consequence, the development of dsAgraLac2-treated insects was compromised, and in cases of severe phenotypic defects, the insects subsequently died. On the contrary, insects subjected to control treatments did not show any visible phenotypic defects in cuticle formation and successfully molted to the pupal and adult stages. Taken together, our data indicate that AgraLac2 is involved in the cuticle tanning process in A. grandis and may be a promising target for the development of RNAi-based technologies.
Published: 2020
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12. Management Strategies for Lamb Production on Pasture-Based Systems in Subtropical Regions: A Review

Author: Alda Lúcia Gomes Monteiro, Cesar Henrique Espirito Candal Poli, Luiza Ilha Borges, Juliano Henriques da Motta, James P. Muir, Thais Devincenzi, Fernando Henrique Melo Andrade Rodrigues de Albuquerque, CESAR HENRIQUE ESPÍRITO CANDAL POLI, Universidade Federal do Rio Grande do Sul (UFRGS) - Porto Alegre, RS, Brazil, ALDA LUCIA GOMES MONTEIRO, Universidade Federal do Paraná (UFPR) - Curitiba, PR, Brazil, THAIS DEVINCENZI, Instituto Nacional de Investigación Agropecuaria (INIA) - Tacuarembó, Uruguay, FERNANDO HENRIQUE MELO ANDRADE RODRIGUES DE ALBUQUERQUE, CNPC, JULIANO HENRIQUES DA MOTTA, Universidade Federal do Rio Grande do Sul (UFRGS) - Porto Alegre, RS, Brazil, LUIZA ILHA BORGES, Universidade Federal do Paraná (UFPR) - Curitiba, PR, Brazil, and JAMES PIERRE MUIR, Texas A&M AgriLife - Stephenville, TX, United States.
Subjects: Animal production, sheep, Wet weather, 040301 veterinary sciences, Review, Subtropics, Pasture management, Pasture, 0403 veterinary science, 03 medical and health sciences, concentrate supplement, Grazing, parasitic diseases, Production (economics), Feed supplements, Pasture based, Creep feeding, 030304 developmental biology, pasture management, 0303 health sciences, geography, geography.geographical_feature_category, lcsh:Veterinary medicine, General Veterinary, biology, Pastures, 04 agricultural and veterinary sciences, Concentrate supplement, biology.organism_classification, Feed concentrates, ewe, Agronomy, Sheep feeding, lcsh:SF600-1100, Veterinary Science, Ewes, performance, Creep grazing, Lamb feeding, Animal performance, Haemonchus contortus
Abstract: Sheep production on pasture plays an important role in subtropical climates around the world, with great economic and environmental relevance to those regions. However, this production is much lower than its true potential in subtropical regions, largely due to lack of knowledge of how to feed grazing lambs, and mitigate gastrointestinal parasite infections. Due to weather instability and the high growth rate of tropical grasses, it is difficult to adjust the quality and quantity of feed consumed by lambs. In addition, due to warm, wet weather during spring, summer, and autumn, gastrointestinal parasite infection can be intense on subtropical pastures. Thus, the objective of this paper is to summarize 17 years of research in southern regions of Brazil testing alternative management for sheep farmers under these challenging conditions. Our review indicates that ewes play important roles raising their lambs. Besides protecting and providing milk, they leave a better pasture structure for lamb nutrition. The use of creep feeding and creep grazing are additional alternatives to improve lamb growth. However, feeding supplementation with concentrate can deteriorate pasture quality at the end of the summer?autumn season. Gastrointestinal parasitic infections can be reduced with improved lamb nutrition, although L3 larvae of Haemonchus contortus can be present at various pasture heights. This indicates that it is difficult to control L3 ingestion solely by manipulating grazing heights. We summarize important technologies for raising lambs on pasture-based systems to make the best of high herbage growth and minimize intense parasitic infections common in subtropical regions. We discuss research results in light of the latest studies from other ecoregions and climates, although there is a lack of similar research in subtropical regions of the world. Made available in DSpace on 2020-09-24T09:12:04Z (GMT). No. of bitstreams: 1 CNPC-2020-Art-18.pdf: 269365 bytes, checksum: 34b8b416c5d869df96ea11e992d8e96e (MD5) Previous issue date: 2020
Published: 2020
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13. Somatic embryogenesis as an alternative for in vitro multiplication of Butia odorata from mature zygotic embryos

Author: Claudimar Sidnei Fior, Jonny Everson Scherwinski-Pereira, Regina Beatriz Bernd, Sergio F Schwaz, Samanta Siqueira de Campos, SAMANTA S. DE CAMPOS, UFRGS, JONNY EVERSON SCHERWINSKI PEREIRA, Cenargen, REGINA B. BERND, CLAUDIMAR S. FIOR, UFRGS, and SERGIO F. SCHWAZ, UFRGS.
Subjects: 0106 biological sciences, Plant Somatic Embryogenesis Techniques, picloram, Somatic embryogenesis, Science, Picloram, Embryonic Development, Biology, Arecaceae, 2,4-D, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, somatic embryo, Auxin, Botany, Butia, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Multidisciplinary, Zygote, Embryogenesis, Embryo, biology.organism_classification, chemistry, Callus, Uruguay, pindo palm, Brazil, 010606 plant biology & botany
Abstract: Butia odorata is a palm native to southern Brazil and Uruguay, not domesticated, much appreciated for its fruits and economic potential. However, the extractivism and the difficulty of propagation have led to the decline of natural populations. The objective of this work was to prove the possibility of induction of somatic embryogenesis in B. odorata. Mature zygotic embryos were induced in two media, MS and Y3, combined with auxin 2,4-D and picloram in five concentrations (2,4-D: 0, 361.99, 452.49, 542.99 and 633.48 μM/L, picloram: 0, 50, 150, 300 and 450 μM/L). The results promising during induction with the formation of embryogenic calli and somatic embryos, however the regeneration of them was not efficient, this may be due to the occurrence of somatic embryos fused during its development. The roots were formed, but the aerial part remained molten, not completing its development. Auxin picloram and Y3 medium provided the most adequate conditions for calogenesis, formation of embryogenic callus and somatic embryos, with concentrations of 150, 300 and 450 μM/L. This is the first description of somatic embryogenesis in B. odorata that will serve as the basis for future research and adjustments of the methodology proposed here.
Published: 2020

14. A Novel Divergent Geminivirus Identified in Asymptomatic New World Cactaceae Plants

Author: Anthony Khalifeh, Christian Vernière, Kendal Smith, Michael C. Lund, Simona Kraberger, Kara Schmidlin, Rafaela S. Fontenele, Amulya Bhaskara, Darren P. Martin, Ilaria N. Cobb, Andrew M. Salywon, Arvind Varsani, Raul Puente-Martinez, Simone G. Ribeiro, Koenraad Van Doorslaer, Gerardo R. Argüello-Astorga, Martin F. Wojciechowski, Denis Filloux, Lucas C. Majure, Safaa G. Kumari, Matias Köhler, Jesús Aarón Avalos-Calleros, Pierre Lefeuvre, Philippe Roumagnac, Joshua Schreck, Wendy C. Hodgson, Arizona State University [Tempe] (ASU), Desert Botanical Garden, University of Florida [Gainesville] (UF), University of Northern British Columbia [Prince George] (UNBC), Instituto Potosino de Investigacion Cientifica y Tecnologica (IPICYT), Consejo Nacional de Ciencia y Tecnología [Mexico] (CONACYT), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), International Center for Agricultural Research in the Dry Areas [Jordanie] (ICARDA), Biologie et Génétique des Interactions Plante-Parasite (UMR BGPI), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Département Systèmes Biologiques (Cirad-BIOS), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Peuplements végétaux et bioagresseurs en milieu tropical (UMR PVBMT), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD)-Université de La Réunion (UR)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Embrapa Recursos Genéticos e Biotecnologia [Brasília], University of Cape Town, Arizona State University, USA, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), RAFAELA S. FONTENELE, ARIZONA STATE UNIVERSITY, USA, ANDREW M. SALYWON, DESERT BOTANICAL GARDEN, USA, LUCAS C. MAJURE, DESERT BOTANICAL GARDEN, USA, ILARIA N. COBB, ARIZONA STATE UNIVERSITY, USA, AMULYA BHASKARA, ARIZONA STATE UNIVERSITY, USA, JESÚS A. AVALOS-CALLEROS, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO, GERARDO R. ARGÜLLO-ASTORGA, INSTITUTO POTOSINO DE INVESTIGACIÓN CIENTÍFICA Y TECNOLÓGICA, MEXICO, KARA SCHMIDLIN, ARIZONA STATE UNIVERSITY, USA, ANTHONY KHALIFEH, ARIZONA STATE UNIVERSITY, USA, KENDAL SMITH, ARIZONA STATE UNIVERSITY, USA, JOSHUA SCHRECK, ARIZONA STATE UNIVERSITY, USA, MICHAEL C. LUND, ARIZONA STATE UNIVERSITY, USA, MATIAS KÖHLER, UFRGS, MARTIN F. WOJCIECHOWSKI, ARIZONA STATE UNIVERSITY, USA, WENDY C. HODGSON, DESERT BOTANICAL GARDEN, USA, RAUL PUENT-MARTINEZ, DESERT BOTANICAL GARDEN, USA, KOENRAAD VAN DOORSLAER, UNIVERSITY OF ARIZONA, USA, SAFAA KUMARI, ICARDA, CHRISTIAN VERNIÈRE, CIRAD, FRANCE, DENIS FILLOUX, CIRAD, FRANCE, PHILIPPE ROUMAGNAC, CIRAD, FRANCE, PIERRE LEFEUVRE, CIRAD, FRANCE, SIMONE DA GRACA RIBEIRO, Cenargen, SIMONA KRABERGER, ARIZONA STATE UNIVERSITY, USA, DARREN P. MARTIN, UNIVERSITY OF CAPE TOWNM, SOUTH AFRICA, and ARVIND VARSANI, ARIZONA STATE UNIVERSITY, USA.
Subjects: Cactaceae, 0106 biological sciences, 0301 basic medicine, Opuntia microdasys, [SDV]Life Sciences [q-bio], lcsh:QR1-502, SsDNA virus, 01 natural sciences, Genome, lcsh:Microbiology, ssDNA viruses [EN], Genus, Opuntioideae, Clade, Phylogeny, Cactoideae, Recombination, Genetic, Dactylopius, Geminiviridae, Infectious Diseases, ssDNA virus, Cactus, Geminivirus, cochineal insects, geminivirus, Genome, Viral, Biology, Article, Hemiptera, 03 medical and health sciences, Virology, Tobacco, Animals, Cochineal insects, Mexico, Géminivirus, Plant Diseases, H20 - Maladies des plantes, biology.organism_classification, United States, 030104 developmental biology, Evolutionary biology, 010606 plant biology & botany
Abstract: Cactaceae comprise a diverse and iconic group of flowering plants which are almost exclusively indigenous to the New World. The wide variety of growth forms found amongst the cacti have led to the trafficking of many species throughout the world as ornamentals. Despite the evolution and physiological properties of these plants having been extensively studied, little research has focused on cactus-associated viral communities. While only single-stranded RNA viruses had ever been reported in cacti, here we report the discovery of cactus-infecting single-stranded DNA viruses. These viruses all apparently belong to a single divergent species of the family Geminiviridae and have been tentatively named Opuntia virus 1 (OpV1). A total of 79 apparently complete OpV1 genomes were recovered from 31 different cactus plants (belonging to 20 different cactus species from both the Cactoideae and Opuntioideae clades) and from nine cactus-feeding cochineal insects (Dactylopius sp.) sampled in the USA and Mexico. These 79 OpV1 genomes all share &gt, 78.4% nucleotide identity with one another and &lt, 64.9% identity with previously characterized geminiviruses. Collectively, the OpV1 genomes display evidence of frequent recombination, with some genomes displaying up to five recombinant regions. In one case, recombinant regions span ~40% of the genome. We demonstrate that an infectious clone of an OpV1 genome can replicate in Nicotiana benthamiana and Opuntia microdasys. In addition to expanding the inventory of viruses that are known to infect cacti, the OpV1 group is so distantly related to other known geminiviruses that it likely represents a new geminivirus genus. It remains to be determined whether, like its cactus hosts, its geographical distribution spans the globe.
Published: 2020
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15. Copaiba oil-resin (Copaifera reticulata Ducke) modulates the inflammation in a model of injury to rats’ tongues

Author: Roberta Souza D'Almeida Couto, Francisco Bruno Teixeira, Manoela Domingues Martins, Rafael Rodrigues Lima, Osmar Alves Lameira, Liana Preto Webber, Raíra de Brito Silva, Francisco Bruno Teixeira, UFPA, Raíra de Brito Silva, UFPA, OSMAR ALVES LAMEIRA, CPATU, Liana Preto Webber, UFRGS, Roberta Souza D'Almeida Couto, UFPA, Manoela Domingues Martins, UFRGS, and Rafael Rodrigues Lima, UFPA.
Subjects: Male, 0301 basic medicine, Model of injury, Pathology, Fitoterapia, Macrophage, Anti-Inflammatory Agents, Pharmacology, Copaiba Oil, Tongue Diseases, 0302 clinical medicine, Medicine, Tissue homeostasis, biology, Fabaceae, General Medicine, lcsh:Other systems of medicine, Copaíba, 030220 oncology & carcinogenesis, Inflamacoes, medicine.symptom, Research Article, medicine.medical_specialty, Copaifera Reticulata Ducke, Copaifera, Inflammation, Amazonian biodiversity, 03 medical and health sciences, Copaiba, Animals, Humans, Plant Oils, Rats, Wistar, Wound Healing, Plant Extracts, business.industry, Copaiba oil-resin, biology.organism_classification, lcsh:RZ201-999, Acute toxicity, Complementary therapies, Rats, Planta Medicinal, 030104 developmental biology, Complementary and alternative medicine, Patologia bucal, Chronic Inflammatory Infiltrate, business, Wound healing, Resins, Plant
Abstract: Background The regeneration of integrity and tissue homeostasis after injury is a fundamental property and involves complex biological processes fully dynamic and interconnected. Although there are medications prescribed to accelerate the process of wound healing by reducing the exaggerated inflammatory response, comes the need to search for different compounds of Amazonian biodiversity that can contribute to the acceleration of the healing process. Among these products, the copaiba oil-resin is one of the most prominent feature in this scenario, as they have been reported its medicinal properties. Methods Aiming to evaluate the anti-inflammatory and healing effect of copaiba oil-resin (Copaifera reticulata Ducke) in transfixing injury of rats’ tongues first proceeded up the copaiba oil-resin oral toxicity test in 5 male mice to stipulate the therapeutic dose which was established at 200 mg/kg/day. Then it was induced transfixing injury in a total of 15 Wistar rats. The animals were randomly divided into three groups based on the treatment: control group, dexamethasone group and copaiba oil-resin group. After 7 days of treatment, histological slides stained with hematoxylin and eosin was prepared. Immunohistochemistry for CD68 (macrophage marker) was performed and analyzed by the cell counter Image J. Results The acute toxicity test showed that the oil-resin copal has low toxicity. Furthermore, copaiba oil-resin therapy modulates the inflammatory response by decreasing the chronic inflammatory infiltrate, edema and specifically the number of macrophages. Conclusions The results indicate the potential of the Amazon region and showed up relevant because therapy with this extract modulates the inflammatory process.
Published: 2017
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16. Effect of green tea and oregano extracts fed to preweaned Jerseycalves on behavior and health status

Author: Dejani Maira Panazzolo, Guilherme Heisler, Vivian Fischer, Micheli de Paris, Maira Balbinotti Zanela, I. D. V. Angelo, GUILHERME HEISLER, UFRGS, VIVIAN FISCHER, UFRGS, MICHELI DE PARIS, UFRGS, ISABELLE DAMÉ VEBER ANGELO, UFRGS, DEJANI MAIRA PANAZZOLO, UFRGS, MAIRA BALBINOTTI ZANELA, CPACT., GUILHERME HEISLER, VIVIAN FISCHER, MICHELI DE PARIS, ISABELLE DAMÉ VEBER ANGELO, and DEJANI MAIRA PANAZZOLO
Subjects: Chá Verde, General Veterinary, 040301 veterinary sciences, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Green tea extract, Origanum, Gado Jersey, Straw, Biology, biology.organism_classification, 040201 dairy & animal science, 0403 veterinary science, Animal science, Orégano, Bezerro, Rumination, medicine, Ingestion, Camellia sinensis, medicine.symptom, Ruminating, Ruminante, Weight gain
Abstract: The aim of this study was to evaluate the effects of green tea (Camellia sinensis) and oregano (Origanum vulgare) extracts on behavior, weight gain, and health indicators of dairy calves from birth to 60 days of age. Twenty-one Jersey calves were distributed into three treatments: without additives (CON); green tea extract (GT): supply of 30 mg/kg of body weight/day; and oregano extract (OR): supply of 60 mg/kg of body weight/day. Observers registered animal?s behavior at 5-minute intervals from 8:00 to 16:00, three times a week, from day 1 to day 60 of life. The behavioral variables considered were time spent in standing, lying down, ruminating, frequency of visits to feeders, frequency of eating pasture and straw, as well as body weight gain, body temperature, heart and respiratory rates, and health conditions. GT and OR anticipated the occurrence of the first rumination and the ingestion of straw in approximately 7 days compared with CON but did not modify the body weight gain, incidence of diarrhea, health conditions, and other behavioral variables. Supply of GT and OR stimulated the onset of dry feed intake and rumination without changing performance and health status. Made available in DSpace on 2020-05-23T04:40:02Z (GMT). No. of bitstreams: 1 Heisler-2020.pdf: 519556 bytes, checksum: aeccb4ba4631d131377121d7b1c82ac1 (MD5) Previous issue date: 2020
Published: 2020

17. Evaluation of two strategies for reducing the spread of Salmonella in commercial swine herds during the finishing phase and their incremental cost-effectiveness ratios

Author: Nelson Mores, Luis Gustavo Corbellini, Armando Lopes do Amaral, Jalusa Deon Kich, Marcelo Miele, Marisa Ribeiro de Itapema Cardoso, Arlei Coldebella, Eduardo de Freitas Costa, EDUARDO DE FREITAS COSTA, UFRGS, JALUSA DEON KICH, CNPSA, MARCELO MIELE, CNPSA, NELSON MORÉS, CNPSA, ARMANDO LOPES DO AMARAL, CNPSA, ARLEI COLDEBELLA, CNPSA, MARISA RIBEIRO DE ITAPEMA CARDOSO, UFRGS, and LUIS GUSTAVO CORBELLINI, UFRGS.
Subjects: Prebióticos, Salmonella, Veterinary medicine, Swine, animal diseases, medicine.medical_treatment, Suínos, Prebiotic, Biology, medicine.disease_cause, Análise de custo-benefício, Prevenção e controle, Untreated control, medicine, Seroprevalence, Custo-efetividade, Prebiótica, Financial impact, Carcass contamination, Contamination, Vacina, Finishing herds, Vacinas, Herd, Cost-effectiveness, Salmonelose, Suíno, Produção animal, General Agricultural and Biological Sciences, Vaccine
Abstract: Para alcançar controle da contaminação por Salmonella em carcaças suínas, intervenções na produção primária precisam ser melhor compreendidas. Estratégias complementares requerem não só pesquisas acerca da sua efetividade, mas também dos custos implicados no uso de tais tecnologias. Para tanto, foi avaliada a razão de custo-efetividade incremental de dois tratamentos para reduzir a soroprevalência de Salmonella em rebanhos suínos comerciais. O uso de um prebiótico e de uma vacina foram comparados com um controle sem tratamento. Cada estratégia foi aplicada em três lotes de suínos em um sistema comercial de integração. Os animais foram acompanhados da maternidade até o abate e suas sorologias no dia do alojamento na terminação e quatro dias antes do abate foram avaliadas. Também, em cada estratégia, amostras de suabe de carcaça foram coletadas para avaliação da contaminação superficial. A soroprevalência no dia do alojamento na terminação foi menor do que 3% em todos os grupos, sendo que nos grupos controle e vacina a soroprevalência aumentou mais de 90 pontos percentuais quatro dias antes do abate. Apenas o uso do prebiótico levou a um efeito significativo na redução da Soroprevalência pré-abate (49 pontos percentuais), quando comparado com o controle. A contaminação das carcaças no grupo prebiótico foi 0%, 18,33% no controle e 29,16 no grupo vacinado. Assim, apenas o prebiótico foi capaz de reduzir a soroprevalência nos rebanhos estudados com razão incremental de custo-efetividade de 1,92 USD para redução de 10 pontos percentuais na soroprevalência por tonelada de carcaça. To achieve control of Salmonella contamination in pig carcasses, on-farm measures need to be better understood. Complementary strategies require research not only on their effectiveness but also on their financial impact. In this study, we evaluated the incremental cost-effectiveness ratio of two treatments for reducing Salmonella seroprevalence in commercial swine herds. Pigs treated with a prebiotic or a vaccine were studied and compared with pigs in an untreated control group. Each strategy was applied to three batches of pigs in a commercial integration system; the animals were followed from farrowing to the slaughterhouse, and their serologies upon arrival at finishing farms and before slaughter were evaluated. Additionally, carcass surface contamination was assessed for each strategy. The seroprevalence upon arrival at the finishing farm was lower than 3% in all groups. In the control and vaccine groups, the seroprevalence increased by more than 90 percentage points from the day of arrival at the finishing farm to four days before slaughter. Only the prebiotic treatment yielded a significant effect on preslaughter seroprevalence (a 49 percentage points reduction from that in the control). Carcass contamination was 0% in the prebiotic group, 18.33% in the control group and 29.16% in the vaccine group. Only prebiotics significantly reduced the seroprevalence of Salmonella in the studied herds, and the incremental cost-effectiveness ratio associated with prebiotic use was 1.92 USD to reduce seroprevalence by 10 percentage points per carcass ton.
Published: 2020

18. Insights Into Genetic and Molecular Elements for Transgenic Crop Development

Author: M. F. GROSSI-de-SÁ, Marcio Alves-Ferreira, Fabricio B. M. Arraes, Maria Fatima Grossi-de-Sa, Valdeir Junio Vaz Moreira, Marcos Fernando Basso, MARCOS FERNANDO BASSO, FABRÍCIO BARBOSA MONTEIRO ARRAES, UFRGS, MAÍRA GROSSI-DE-SA, VALDEIR JUNIO VAZ MOREIRA, UFRGS, MARCIO ALVES-FERREIRA, UFRJ, and MARIA FATIMA GROSSI DE SA, Cenargen.
Subjects: 0106 biological sciences, 0301 basic medicine, Plant Science, lcsh:Plant culture, Biology, Plant genetic engineering, 01 natural sciences, Plant genetic transformation, plant genetic transformation, Crop, 03 medical and health sciences, T-DNA delivery, lcsh:SB1-1110, Plant breeding, tissue culture, Original Research, minimal expression cassette, new biotechnological tools, Minimal expression cassette, business.industry, Techniques of genetic engineering, New biotechnological tools, Biotechnology, Tissue culture, 030104 developmental biology, Agriculture, Trait, business, 010606 plant biology & botany
Abstract: Climate change and the exploration of new areas of cultivation have impacted the yields of several economically important crops worldwide. Both conventional plant breeding based on planned crosses between parents with specific traits and genetic engineering to develop new biotechnological tools (NBTs) have allowed the development of elite cultivars with new features of agronomic interest. The use of these NBTs in the search for agricultural solutions has gained prominence in recent years due to their rapid generation of elite cultivars that meet the needs of crop producers, and the efficiency of these NBTs is closely related to the optimization or best use of their elements. Currently, several genetic engineering techniques are used in synthetic biotechnology to successfully improve desirable traits or remove undesirable traits in crops. However, the features, drawbacks, and advantages of each technique are still not well understood, and thus, these methods have not been fully exploited. Here, we provide a brief overview of the plant genetic engineering platforms that have been used for proof of concept and agronomic trait improvement, review the major elements and processes of synthetic biotechnology, and, finally, present the major NBTs used to improve agronomic traits in socioeconomically important crops.
Published: 2019

19. Taxonomic Status and Population Oscillations of Aphidius colemani Species Group (Hymenoptera: Braconidae) in Southern Brazil

Author: Carlos D. R. Santos, Douglas Lau, Marcus Vinicius Sampaio, J. Pivato, Simone Mundstock Jahnke, Fábio Janoni Carvalho, Luiza Rodrigues Redaelli, C. D. R. D. Santos, Posgraduate Program in Plant Science, Faculty of Agronomy, Federal Univ of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul,Brasil, M. V. Sampaio, Agricultural Sciences Institute, Federal Univ of Uberlândia (UFU), Uberlândia, Minas Gerais, Brasi, DOUGLAS LAU, CNPT, L. R. Redaelli, Posgraduate Program in Plant Science, Faculty of Agronomy, Federal Univ of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul,Brasil, Jahnke, S. M., Posgraduate Program in Plant Science, Faculty of Agronomy, Federal Univ of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul,Brasil, J. Pivato, Faculty of Agronomy, Passo Fundo Univ (UPF), Passo Fundo, Rio Grande do Sul, Brasi, and F. J. Carvalho, Agricultural Sciences Institute, Federal Univ of Uberlândia (UFU), Uberlândia, Minas Gerais, Brasi.
Subjects: 0106 biological sciences, Male, Veterinary medicine, Population, Wasps, Hymenoptera, 010603 evolutionary biology, 01 natural sciences, Population density, Parasitoid, Aphidius colemani, Animals, Wings, Animal, Sex Ratio, education, Pest Control, Biological, Triticum, Taxonomy, Population Density, education.field_of_study, Aphid, Parasitoids, biology, Cereal aphid, Temperature, biology.organism_classification, Monitoring program, 010602 entomology, Biological Control Agents, Insect Science, Aphids, Cryptic taxa, Female, Seasons, Braconidae, Sex ratio, Brazil
Abstract: Aphidius colemani(Viereck) was reported in Brazil before the BiologicalControl Program of Wheat Aphids (BCPWA) when Mediterranean genotypeswere introduced from France and Israel. This species was re-described as acomplex calledA. colemanigroup composed of three species. Consequently,uncertainty remains about which parasitoid of the group is occurring insouthern Brazil. This study has two main objectives: (i) re-examine the spe-cies status ofA. colemanigroup collected during the introduction of para-sitoids and from a 10-year (2009?2018) monitoring program in wheat fieldsin northern Rio Grande do Sul (RS), Brazil; (ii) describe the variation in thepopulation density of parasitoids andits association with meteorologicalfactors during this period. We examined 116 specimens from the EmbrapaWheat entomological collection, and those collected in Moericke traps inCoxilha, RS. All the parasitoids of theA. colemanigroup from the BCPWAperiod were identified asAphidius platensis(Brèthes). In traps, 6541 cerealaphid parasitoids were collected, of which 61.9% (n=4047)werefromA. colemanigroup and all those were identified asA. platensis.Temperature was the factor that effected population density with the high-est number of parasitoids recorded inthe winter months. Sex ratio changedbetween years varying from 0.50 to 0.97. The parasitoidA. platensiswas theonly species in theA. colemanigroup sampled during 10 years of monitoring Made available in DSpace on 2020-02-05T00:37:12Z (GMT). No. of bitstreams: 1 Santos2019ArticleTaxonomicStatusAndPopulationOs.pdf: 1176910 bytes, checksum: 76441294c9acaf166739d82fa08eb982 (MD5) Previous issue date: 2019
Published: 2019

20. Gene expression analysis reveals important pathways for drought response in leaves and roots of a wheat cultivar adapted to rainfed cropping in the Cerrado biome

Author: Rafael Augusto Arenhart, Jorge Fernando Pereira, Luciano Consoli, Gonzáles Hh, Márcia Margis-Pinheiro, L. B. Poersch-Bortolon, G. A. M. Torres, Nhani A Junior, Maria Helena Bodanese-Zanettini, LIANE BALVEDI POERSCH-BORTOLON, UFRGS, JORGE FERNANDO PEREIRA, CNPT, ANTONIO NHANI JUNIOR, CNPT, HEBERT HERNÁN SOTO GONZÁLES, UFRR, GISELE ABIGAIL MONTAN TORRES, CNPT, LUCIANO CONSOLI, CNPT, RAFAEL AUGUSTO ARENHART, UFRGS, MARIA HELENA BODANESE-ZANETTINI, UFRGS, and MÁRCIA MARGIS-PINHEIRO, UFRGS.
Subjects: 0106 biological sciences, 0301 basic medicine, Candidate gene, lcsh:QH426-470, Biome, Triticum aestivum, Trigo, Biology, Plant Genetics, 01 natural sciences, Genome, Genes candidatos, PCR quantitativo, 03 medical and health sciences, Botany, Gene expression, Genetics, Cultivar, Triticum Aestivum, Molecular Biology, Gene, water deficit, Deficits hidricos, RT-qPCR, 454 sequencing, food and beverages, Tolerância à seca, lcsh:Genetics, Metabolic pathway, 030104 developmental biology, Reação em cadeia da polimerase em tempo real, Déficit hídrico, Pyrosequencing, Sequenciamento de nova geração, candidate genes, 010606 plant biology & botany
Abstract: Drought limits wheat production in the Brazilian Cerrado biome. In order to search for candidate genes associated to the response to water deficit, we analyzed the gene expression profiles, under severe drought stress, in roots and leaves of the cultivar MGS1 Aliança, a well-adapted cultivar to the Cerrado. A set of 4,422 candidate genes was found in roots and leaves. The number of down-regulated transcripts in roots was higher than the up-regulated transcripts, while the opposite occurred in leaves. The number of common transcripts between the two tissues was 1,249, while 2,124 were specific to roots and 1,049 specific to leaves. Quantitative RT-PCR analysis revealed a 0.78 correlation with the expression data. The candidate genes were distributed across all chromosomes and component genomes, but a greater number was mapped on the B genome, particularly on chromosomes 3B, 5B and 2B. When considering both tissues, 116 different pathways were induced. One common pathway, among the top three activated pathways in both tissues, was starch and sucrose metabolism. These results pave the way for future marker development and selection of important genes and are useful for understanding the metabolic pathways involved in wheat drought response.
Published: 2016
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21. Evolutionary and social consequences of introgression of nontransgenic herbicide resistance from rice to weedy rice in Brazil

Author: Alcido Elenor Wander, Aldo Merotto, Valmir Gaedke Menezes, Augusto Kalsing, Anderson Luis Nunes, Catarine Markus, Ives Clayton Gomes dos Reis Goulart, Aldo Merotto Junior, UFRGS, IVES CLAYTON GOMES DOS REIS GOULART, CNPF, Anderson L. Nunes, IFRS, Augusto Kalsing, Dow AgroSciences, Catarine Markus, UFRGS, Valmir G. Menezes, IRGA, and ALCIDO ELENOR WANDER, CNPAF.
Subjects: 0106 biological sciences, Clearfield TM, Imazethapyr, outcrossing, Population, Biodiversity, Introgression, Fluxo de genes, ClearfieldTM, Biology, Outcrossing, 01 natural sciences, imazethapyr, Gene flow, Fitness, herbicide resistance, Genetics, Cultivar, education, Ecology, Evolution, Behavior and Systematics, education.field_of_study, business.industry, Red rice, food and beverages, 04 agricultural and veterinary sciences, fitness, Biotechnology, Herbicide resistance, Agronomy, Arroz, Agriculture, acetolactate synthase, Herbicida, Acetolactate synthase, 040103 agronomy & agriculture, red rice, 0401 agriculture, forestry, and fisheries, gene flow, General Agricultural and Biological Sciences, business, Reviews and Syntheses, 010606 plant biology & botany, Weedy rice
Abstract: Several studies have expressed concerns about the effects of gene flow from transgenic herbicide‐resistant crops to their wild relatives, but no major problems have been observed. This review describes a case study in which what has been feared in transgenics regarding gene flow has actually changed biodiversity and people's lives. Nontransgenic imidazolinone‐resistant rice (IMI‐rice) cultivars increased the rice grain yield by 50% in southern Brazil. This increase was beneficial for life quality of the farmers and also improved the regional economy. However, weedy rice resistant to imidazolinone herbicides started to evolve three years after the first use of IMI‐rice cultivars. Population genetic studies indicate that the herbicide‐resistant weedy rice was mainly originated from gene flow from resistant cultivars and distributed by seed migration. The problems related with herbicide‐resistant weedy rice increased the production costs of rice that forced farmers to sell or rent their land. Gene flow from cultivated rice to weedy rice has proven to be a large agricultural, economic, and social constraint in the use of herbicide‐resistant technologies in rice. This problem must be taken into account for the development of new transgenic or nontransgenic rice technologies.
Published: 2016
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22. Survival analysis of productive life in Brazilian holstein using a piecewise Weibull proportional hazard model

Author: Claudio Napolis Costa, Jaime Araujo Cobuci, Elisandra Lurdes Kern, Vincent Ducrocq, UFRGS, Departamento de Zootecnia, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Brazilian Agricultural Research Corporation (Embrapa), Génétique Animale et Biologie Intégrative (GABI), Institut National de la Recherche Agronomique (INRA)-AgroParisTech, and Coordination of Improvement of Higher Education Personnel (CAPES)
Subjects: genetical analysis, 0301 basic medicine, Environment effect, [SDV]Life Sciences [q-bio], animal diseases, Ice calving, Culling, Biology, survival, 03 medical and health sciences, Animal science, longevity, Lactation, medicine, longévité, 2. Zero hunger, General Veterinary, Proportional hazards model, Genetic analysis, Sire, dairy cattle, 0402 animal and dairy science, food and beverages, effet de l'environnement, 04 agricultural and veterinary sciences, Heritability, Random effects model, veterinary(all), 040201 dairy & animal science, survie, bovin laitier, 030104 developmental biology, medicine.anatomical_structure, Herd, Animal Science and Zoology, analyse génétique
Abstract: The objectives of this study were to assess the most important factors that influence productive life (PL) of Brazilian Holstein cows and to estimate genetic parameters for PL using a piecewise Weibull proportional hazard model. Records of PL from first calving to last recording (culling) of 132,922 cows coming from 945 herds were used. They had to have their first calving occurring between 1989 and 2013 and they were daughters of 6,804 sires. The model included the time-dependent effects of region within year, class of milk production within herd-year, class of milk production within lactation number, class of fat and protein contents within herd and (variation in) herd size as well as the time-independent fixed effect of age at first calving, the random effects of herd-year, sire and maternal grand sire. All fixed effects had a significant effect on PL (P < 0.001). The relative risk (RR) for within herd class of milk yield varied from 3.16 for the worst 20% class to 0.41 for the best 20% class. RR also increased as protein and fat decreased, but to a lesser extent compared to milk yield. Significant effects on PL were found for region year, with large yearly changes in some cases. RR increased with age at first calving and with herd size but lower risks were observed when herd size was increasing or decreasing, compared to stable herds. The Weibull shape parameters (and therefore RR) increased with lactation number and with stage of lactation. The sire genetic variance estimate was 0.030 +/- 0.002 which corresponds to an equivalent heritability estimate of 6.1% accounting for censoring. A positive genetic trend of PL was observed. These results may contribute to the development of a routine genetic evaluation necessary to improve PL of Brazilian Holsteins.
Published: 2016
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23. Calves fed with milk from cows receiving plant extracts improved redox status

Author: Caroline Peres Klein, L.T. Passos, Guilherme Heisler, Vinícius Stone, M.F. de Paris, Maira Balbinotti Zanela, Vivian Fischer, E.F. Vizzotto, Sheila Cristina Bosco Stivanin, Cristiane Matté, I. D. V. Angelo, M. DE PARIS, UFRGS, S. C. B. STIVANIN, UFRGS, C. P. KLEIN, UFRGS, E. F. VIZZOTTO, UFRGS, L. T. PASSOS, UFRGS, I. D. V. ANGELO, UFRGS, MAIRA BALBINOTTI ZANELA, CPACT, V. STONE, UFRGS, C. MATTÉ, UFRGS, G. HEISLER, UFRGS, and V. FISCHER, UFRGS.
Subjects: Chá Verde, 0301 basic medicine, Antioxidant, Every Two Weeks, GPX3, medicine.medical_treatment, Gado Jersey, Green tea extract, 03 medical and health sciences, chemistry.chemical_compound, Orégano, Animal science, Bezerro, medicine, Antioxidante, Feces, Completely randomized design, General Veterinary, biology, 0402 animal and dairy science, 04 agricultural and veterinary sciences, Origanum, Glutathione, biology.organism_classification, 040201 dairy & animal science, 030104 developmental biology, chemistry, Animal Science and Zoology
Abstract: The objective of this study was to evaluate the biomarkers of the redox state of pre-weaned Jersey dairy calves that consumed milk from cows supplemented with green tea (Camellia sinensis) or oregano extracts (Origanum vulgare). A completely randomized design was used with repeated measures in time. From their birth to 60 days of life, 8, 8 and 7 calves received milk from cows fed on a basal diet without addition of plant extracts (CON), with addition of 10.0 g of oregano extract (OE) per day and with 5.0 g of green tea extract (GT) per day, respectively. On days 1, 30, and 60 after birth redox state biomarkers were evaluated. Body weight was eval-uated every two weeks, rectal temperature and fecal score were accessed every two days and concentrate intake was measured every day. Statistical analyses for body weight, body weight gain, concentrate intake, rectal temperature, variables of redox status were performed using the procedure Mixed, evaluating the fixed effects of treatment, day of measurement and their interaction. Statistical analyses for the mean fecal score and the number of days to the first diarrhea occurrence were performed using the procedure Mixed, evaluating the fixed effect of treatment. On day 1, calves fed with milk from GT group had higher plasma glutathione peroxidase activity (GPx) than CON. However, the reverse occurred on day 60. On the day 1, calves in the CON group presented higher concentrations of thiol groups (also known as sulfhydryl groups) than those in GT and OE groups, with reverse occurring on day 30. Calves in the OE group had lower oxidation of dichlorofluorescein in the erythrocytes compared to the others; while calves in the GT group presented higher concentration of GSH and higher activity of the catalase enzyme compared to CON and OE, respectively. Pre-weaned calves fed with milk of cows supplemented with extracts of green tea and oregano did not change... Made available in DSpace on 2020-12-02T09:02:50Z (GMT). No. of bitstreams: 1 Paris-2020-Livestock-Science.pdf: 468473 bytes, checksum: 9b39777d6f7a8090f464df337801e354 (MD5) Previous issue date: 2020
Published: 2020
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24. First First record of Phaenoglyphis villosa (Hartig, 1841) in Brazil (Hymenoptera: Cynipoidea: Figitidae: Charipinae)

Author: Carlos D. R. Santos, Simone Mundstock Jahnke, Marcus Vinicius Sampaio, Douglas Lau, Paulo Roberto Valle da Silva Pereira, Luiza Rodrigues Redaelli, Mar Ferrer-Suay, CARLOS D. R. SANTOS, Posgraduate Program in Plant Science, Faculty of Agronomy, Federal Univ of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil, DOUGLAS LAU, CNPT, PAULO ROBERTO VALLE DA S PEREIRA, CNPF., MARCUS V. SAMPAIO, Agricultural Sciences Institute, Federal Univ of Uberlândia (UFU), Uberlândia, Minas Gerais, Brazil, MAR FERRER-SUAY, Museum Valencià d'Història Natural & Biotaxa, l?Hort de Feliu-Alginet, Apdo. 8460, E-46018, Valencia, España, and SIMONE M. JAHNKE, Posgraduate Program in Plant Science, Faculty of Agronomy, Federal Univ of Rio Grande do Sul (UFRGS), Porto Alegre, Rio Grande do Sul, Brazil
Subjects: Figitidae, Hymenoptera, Charipinae, Cereal aphids, cereal aphids, Rhopalosiphum padi, Sitobion avenae, wheat, lcsh:Zoology, Botany, lcsh:QL1-991, Aphidiinae, trophic interactions, Hyperparasitoids, biology, Cynipoidea, Aphididae, biology.organism_classification, Wheat, Trophic interactions, hyperparasitoid, Phaenoglyphis villosa, Braconidae
Abstract: Phaenoglyphis villosa (Hartig, 1841) (Hymenoptera: Figitidae: Charipinae) is a secondary endoparasitoid of Aphidiinae (Hymenoptera: Braconidae), a subfamily which are important primary parasitoids of aphids (Hemiptera: Aphididae). It is here registered for the first time in Brazil, collected from primary parasitoids in field-exposed aphids. Phaenoglyphis villosa was recorded during wheat crop season (winter and spring), emerging from Rhopalosiphum padi (Linnaeus, 1758) mummies (n = 35♀). It was also recorded from Sitobion avenae (Fabricius, 1775) mummies, in wheat crop season (late winter) (n = 13♀) and black oat crop season (late autumn) (n = 1♀). We suggest three possible primary braconid parasitoids as hosts to this hyperparasitoid: Aphidius platensis Brèthes, 1913, Aphidius rhopalosiphi de Stefani- Perez, 1902, and Aphidius uzbekistanicus Luzhetzki, 1960. Keywords: hyperparasitoid, cereal aphids, trophic interactions, wheat. Made available in DSpace on 2020-07-02T11:11:47Z (GMT). No. of bitstreams: 1 68-Article-Text-1010-2-10-20200619.pdf: 754456 bytes, checksum: f01590da5bd9282398f82e5e6f955a32 (MD5) Previous issue date: 2020
Published: 2020
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25. Enumeration, antimicrobial resistance and typing of salmonella enterica: profile of strains carried in the intestinal contents of pigs at slaughter in southern Brazil

Author: Tatiana Regina Vieira, Marisa Ribeiro de Itapema Cardoso, Daniel Santos Paim, Gabriela Orosco Werlang, J. D. Kich, Eduardo de Freitas Costa, Caroline Pissetti, DANIEL SANTOS PAIM, UFRGS, CAROLINE PISSETTI, UFRGS, TATIANA REGINA VIEIRA, UFRGS, GABRIELA OROSCO WERLANG, UFRGS, EDUARDO DE FREITAS COSTA, UFRGS, JALUSA DEON KICH, CNPSA, and MARISA RIBEIRO DE ITAPEMA CARDOSO, UFRGS.
Subjects: Veterinary medicine, Salmonella, Faecal excretion, Enterobacteria, Swine, Drug resistance, medicine.disease_cause, Ampicillin, medicine, Pulsed-field gel electrophoresis, Total aerobic mesophilic bacteria, biology, food and beverages, Salmonella enterica, General Medicine, biology.organism_classification, Enterobacteriaceae, Salmonella Typhimurium monophasic variant, Whole genome sequencing, Gentamicin, MCR-1 gene, Suíno, medicine.drug, Food contaminant
Abstract: Background: Despite a strong association between Salmonella isolation and slaughter hygiene, as measured by the Enterobacteriaceae levels on pre-chill carcass surfaces, a high variation in this association was observed between sampling days within the same slaughterhouse. It was hypothesised that in a scenario of high exposure on the farm, batches with a high prevalence of carrier pigs shedding a high number of Salmonella may enhance the risk of contamination on some slaughter days. Thus, the aim of this study was to assess the profile of Salmonella carried in the intestinal contents of slaughter pigs. Materials, Methods & Results: Ten pig batches slaughtered in a slaughterhouse were investigated for the presence of Salmonella. From each pig, the following samples were taken: i. blood collected at bleeding; ii. sponges rubbed on the carcass surface after bleeding and before chilling; iii. fragment of the ileocecal region of the intestine. Serum samples were subjected to a ELISA-Typhimurium test. Sponges were investigated for the presence of Salmonella and total aerobic mesophilic (TAM) and terobacteriaceae (EC) bacterial counts. Salmonella was enumerated in the intestinal contents. Selected Salmonella strains were subjected to an antimicrobial resistance disk diffusion test, macro-restriction with Xba-I (PFGE) and whole genome sequencing (WGS). From the 50 sampled pigs, 96% were positive in the ELISA-Typhimurium test and 64% were Salmonella-positive in the intestinal contents. The amount of Salmonella in the intestinal content samples was highly variable, and the mean log of fitted distributions of Salmonella in the batch ranged from -2.97 to 2.25 cfu.g-1. The slaughter process achieved a logarithmic reduction, ranging from 0.64 to 2.35 log cfu.cm-2 for TAM and from 0.55 to 2.57 log cfu.cm-2 for EC. Salmonella was isolated from 16% of the carcasses after bleeding; this frequency decreased to 8% at the pre-chill step. All positive pre-chill carcasses originated from pigs carrying Salmonella in the intestinal content and from batches with a high number of carrier pigs. Salmonella Typhimurium and its monophasic variant were the most frequent in the intestinal contents and carcasses. Resistance was detected against ampicillin (42.5%), tetracycline (42.5%), sulfonamide (40%), gentamicin (25%) and ciprofloxacin (12.5%). Regarding colistin, 85% of the tested strains were classified as non-susceptible. The monophasic variant S. Typhimurium strains subjected to PFGE and WGS presented different profiles; several antimicrobial resistance genes were identified and all belonged to ST-19. Discussion: In this study, almost all sampled pigs entering the slaughter line had been exposed to Salmonella on the farm and a high number were carrying Salmonella in their guts. While the three batches with Salmonella-positive carcasses at the pre-chill step presented TAM media that was not significantly different from the other batches, there was a higher number of positive pigs carrying Salmonella in their intestinal contents. Moreover, the batch with the highest number of positive carcasses also presented the highest Salmonella mean count in their intestinal contents. The profile of Salmonella carried in the intestinal content of slaughter pigs proved to be highly variable in terms of the frequency, number of bacteria, serovars, antimicrobial resistance, and genotypes. Results indicate that the day-to-day variability in the prevalence and number of Salmonella in the intestinal contents of slaughter batches is likely to influence the frequency of contaminated pre-chill carcasses. Salmonella Typhimurium isolated from the intestinal contents of slaughter pigs may belong to genotypes involved in human disease and may carry several antimicrobial resistance genes. These aspects should be taken into account when planning Salmonella control in swine. Made available in DSpace on 2019-10-31T18:14:00Z (GMT). No. of bitstreams: 1 final9102.pdf: 262646 bytes, checksum: 0c65477537e69c9e49d13aafe1556515 (MD5) Previous issue date: 2019
Published: 2019

26. Spring is coming: genetic analyses of the bud break date locus reveal candidate genes from the cold perception pathway to dormancy release in apple (Malus x domestica Borkh.)

Author: Marcus Vinícius Kvitschal, Amanda Malvessi Cattani, Tiago Sartor, Carolina Tessele, Carla Andréa Delatorre, Marcos Mota do Carmo Costa, Luís Fernando Revers, Roberto C. Togawa, Priscila Grynberg, Sérgio Amorim de Alencar, Vanessa Buffon, Vítor da Silveira Falavigna, Diogo Denardi Porto, Frederico Denardi, Orzenil B. Silva-Junior, Paulo Ricardo Dias de Oliveira, Yohanna Evelyn Miotto, Georgios J. Pappas, Ana Beatriz Costa Czermainski, YOHANNA EVELYN MIOTTO, UFRGS, CAROLINA TESSELE, UFRGS, ANA BEATRIZ COSTA CZERMAINSKI, CNPUV, DIOGO DENARDI PORTO, CPATSA, VÍTOR DA SILVEIRA FALAVIGNA, UFRGS, TIAGO SARTOR, UFRGS, AMANDA MALVESSI CATTANI, UFRGS, CARLA ANDREA DELATORRE, UFRGS, SÉRGIO AMORIM DE ALENCAR, UCB, ORZENIL BONFIM DA SILVA JUNIOR, Cenargen, ROBERTO COITI TOGAWA, Cenargen, MARCOS MOTA DO CARMO COSTA, Cenargen, GEORGIOS JOANNIS PAPPAS JÚNIOR, UNB, PRISCILA GRYNBERG, Cenargen, PAULO RICARDO DIAS DE OLIVEIRA, CNPUV, MARCUS VINÍCIUS KVITSCHAL, EPAGRI, FREDERICO DENARDI, EPAGRI, VANESSA BUFFON, CNPUV, LUÍS FERNANDO REVERS, UFRGS., Universidade Federal do Rio Grande do Sul (UFRGS), Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Amélioration génétique et adaptation des plantes méditerranéennes et tropicales (UMR AGAP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade Católica de Brasília (UCB), Universidade de Brasília (UnB), Empresa de Pesquisa Agropecuária e Extensão Rural de Santa Catarina, Financiadora de Estudos e Projetos (FINEP) : 0107009700, Empresa Brasileira de Pesquisa Agropecuaria (Embrapa) : 02.12.12.003.00.02, Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES -Ministry of Education), CAPES, CAPES : 15/2014 83, and Conselho Nacional de Desenvolvimento Cientifico e Tecnologico (CNPq, Ministry of Science, Technology, Innovation and Communications) : 305122/2014-2
Subjects: 0106 biological sciences, 0301 basic medicine, Candidate gene, Malus, linkage mapping, Bud dormancy, MdoPRE1, apple, Locus (genetics), Plant Science, Quantitative trait locus, Biology, lcsh:Plant culture, 01 natural sciences, 03 medical and health sciences, Annual growth cycle of grapevines, Chilling requirement, Genetic linkage, bud dormancy, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, lcsh:SB1-1110, MdoFLC, Genetics, MdoICE1, Vegetal Biology, Apples, biology.organism_classification, 030104 developmental biology, chilling requirement, Genetic marker, Linkage mapping, Dormancy, Biologie végétale, 010606 plant biology & botany
Abstract: Chilling requirement (CR) for bud dormancy completion determines the time of bud break in apple (Malus × domestica Borkh.). The molecular control of bud dormancy is highly heritable, suggesting a strong genetic control of the trait. An available Infinium II SNP platform for genotyping containing 8,788 single nucleotide polymorphic markers was employed, and linkage maps were constructed in a F1 cross from the low CR M13/91 and the moderate CR cv. Fred Hough. These maps were used to identify quantitative trait loci (QTL) for bud break date as a trait related to dormancy release. A major QTL for bud break was detected at the beginning of linkage group 9 (LG9). This QTL remained stable during seven seasons in two different growing sites. To increase mapping efficiency in detecting contributing genes underlying this QTL, 182 additional SNP markers located at the locus for bud break were used. Combining linkage mapping and structural characterization of the region, the high proportion of the phenotypic variance in the trait explained by the QTL is related to the coincident positioning of Arabidopsis orthologs for ICE1, FLC, and PRE1 protein-coding genes. The proximity of these genes from the most explanatory markers of this QTL for bud break suggests potential genetic additive effects, reinforcing the hypothesis of inter-dependent mechanisms controlling dormancy induction and release in apple trees. Made available in DSpace on 2019-06-12T00:39:12Z (GMT). No. of bitstreams: 1 fpls1000033.pdf: 2246583 bytes, checksum: 39a0aa3363675b7338cebcaa1d270aee (MD5) Previous issue date: 2019
Published: 2019
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27. Antimicrobial susceptibility profile of historical and recent Brazilian pig isolates of Pasteurella multocida

Author: Amanda F. Amaral, João Xavier de Oliveira Filho, Karine Ludwig Takeuti, Raquel Rebelatto, David Emilio Santos Neves de Barcellos, Marisa Ribeiro de Itapema Cardoso, Nelson Morés, Catia Silene Klein, AMANDA F. AMARAL, North Caroline State University, RAQUEL REBELATTO, CNPSA, CATIA SILENE KLEIN, CNPSA, KARINE L. TAKEUTI, UFRGS, JOÃO XAVIER DE OLIVEIRA FILHO, UFRGS, NELSON MORES, CNPSA, MARISA RIBEIRO DE ITAPEMA CARDOSO, UFRGS, and DAVID EMÍLIO SANTOS NEVES de BARCELLOS, UFRGS.
Subjects: Florfenicol, Veterinary medicine, microdiluição em caldo, Swine, animal diseases, Resistance, Suínos, Antimicrobial susceptibility, Pasteurella multocida subsp. multocida, 0403 veterinary science, chemistry.chemical_compound, Resistência a antimicrobianos, Pasteurella multocida, concentração inibitória mínima (CIM), 0303 health sciences, lcsh:Veterinary medicine, Minimum inhibitory concentration, 04 agricultural and veterinary sciences, Antimicrobial, Bacterioses, Pigs, Pneumonia (non-human), medicine.drug, suínos, 040301 veterinary sciences, Tetracycline, Biology, 03 medical and health sciences, Microbroth dilution, otorhinolaryngologic diseases, medicine, Enrofloxacin, Suscetibilidade a antimicrobianos, Pasteurella, Testes de sensibilidade microbiana, Antimicrobianos, General Veterinary, Brazilian pig, 030306 microbiology, Amoxicillin, biology.organism_classification, medicine.disease, Bacteriose, resistência, chemistry, lcsh:SF600-1100, bacterioses, Suíno, Minimum Inhibitory Concentration (MIC)
Abstract: Pasteurella (P.) multocida is the causative agent of pneumonic pasteurellosis in swine, which is commonly associated with the final stages of enzootic pneumonia or porcine respiratory disease complex. Although this syndrome is one of the most common and important diseases of pigs, data on antimicrobial susceptibility of P. multocida isolates are uncommon in Brazil. Therefore, the present study was carried out to determine and to compare antimicrobial susceptibility profile of Brazilian P. multocida isolated from pigs with lesions of pneumonia or pleuritis during two-time periods. Historical isolates (period of 1981 to 1997; n=44) and recent isolates (period of 2011 to 2012; n=50) were used to determine the MIC of amoxicillin, enrofloxacin, florfenicol and tetracycline by microbroth dilution. Florfenicol had the lowest level of resistance for both historical and recent isolates (0% and 6%, respectively), while tetracycline had the highest (20.5% and 34%, respectively). Multi-drug resistance (MDR) to amoxicillin/florfenicol/tetracycline was observed in 6% of recent isolates. There was a significant increase (p˂0.05) in resistance for amoxicillin and enrofloxacin in recent isolates compared with historic isolates (3.8% and 18%, respectively), most likely due to the selective pressure of antimicrobial usage to treat and prevent P. multocida infections. The results of this study showed an increase of isolates resistant to important drugs used in treatment of P. multocida infections in pigs, demonstrating the need for the implementation of rational use of antimicrobials in Brazilian swine industry. RESUMO: Pasteurella (P.) multocida é o agente da pasteurelose pneumônica em suínos, a qual é comumente associada com o estágio final da pneumonia enzoótica suína ou complexo das doenças respiratórias dos suínos. Apesar de ser uma das doenças mais comuns e importantes na suinocultura, dados sobre suscetibilidade antimicrobiana de isolados de P. multocida são raros no Brasil. Dessa forma, o presente estudo foi realizado para determinar e comparar o perfil de suscetibilidade de isolados de P. multocida de suínos com lesões de pneumonia ou pleurite no Brasil durante dois períodos. Isolados históricos (período de 1981 a 1997; n=44) e contemporâneos (período de 2011 a 2012; n=50) foram usados para determinar a concentração inibitória mínima (CIM) de amoxicilina, enrofloxacina, florfenicol e tetraciclina através do teste de microdiluição em caldo. Florfenicol apresentou o menor nível de resistência para ambos os isolados históricos e contemporâneos (0% e 6%, respectivamente), enquanto que tetraciclina apresentou o maior nível de resistência (20.5% e 34%, respectivamente). Resistência a múltiplos antimicrobianos (amoxicilina, florfenicol e tetraciclina) foi observada em 6% dos isolados recentes. Foi observado aumento significativo (p˂0.05) na resistência a amoxicilina e enrofloxacina em isolados recentes comparado com isolados históricos (3.8% e 18%, respectivamente), provavelmente devido à pressão de seleção de antimicrobianos usados no tratamento e prevenção de infecções causadas por P. multocida. Os resultados deste trabalho demostraram o aumento de isolados resistentes a importantes drogas utilizadas no tratamento de infecções causadas por P. multocida em suínos, evidenciando a necessidade da implementação do uso racional de antimicrobianos na suinocultura brasileira.
Published: 2019

28. Contrasting effects of wild arachis dehydrin under abiotic and biotic stresses

Author: Etienne Danchin, Ana Claudia Guerra Araujo, Ana Paula Zotta Mota, C. C. Vinson, Thomas C. R. Williams, Thais Nicolini Oliveira, Marcos Mota do Carmo Costa, Maria Fatima Grossi-de-Sa, Ana Cristina Miranda Brasileiro, Patricia Messenberg Guimaraes, Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Universidade Federal do Rio Grande do Sul (UFRGS), Universidade de Brasília (UnB), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), EMBRAPA, CNPq-Brazilian National Council for Scientific and Technological Development, Special Visitor Researcher program (PVE grant), INCT PlantStress [465480/2014-4], CAPES- Coordination for the Improvement of Higher Education Personnel, FAP-DF- Distrito Federal Research Foundation, ANA PAULA ZOTTA MOTA, UFRGS, CHRISTINA CLEO VINSON, UNB, THOMAS CHRISTOPHER RHYS WILLIAMS, UNB, MARCOS MOTA DO CARMO COSTA, Cenargen, ANA CLAUDIA GUERRA DE ARAUJO, Cenargen, THAIS NICOLINI OLIVEIRA, UNB, MARIA FATIMA GROSSI DE SA, Cenargen, PATRICIA MESSEMBERG GUIMARAES, Cenargen, ANA CRISTINA MIRANDA BRASILEIRO, Cenargen., ETIENNE G. J. DANCHIN, UNIVERSITÉ CÔTE D’AZUR, FRANCE, and Miranda Brasileiro, Ana Cristina
Subjects: 0106 biological sciences, 0301 basic medicine, root-knot nematode, Protein family, Meloidogyne, [SDV]Life Sciences [q-bio], drought, freezing, genome-wide, meloidogyne, arabidopsis, Arabidopsis, Plant Science, Root-knot nematodes, lcsh:Plant culture, 01 natural sciences, Arachis duranensis, 03 medical and health sciences, chemistry.chemical_compound, Freezing, lcsh:SB1-1110, Genome-wide, Abscisic acid, 2. Zero hunger, Abiotic component, Genetics, biology, Drought, Abiotic stress, Jasmonic acid, food and beverages, 15. Life on land, biology.organism_classification, Medicago truncatula, 030104 developmental biology, chemistry, [SDE]Environmental Sciences, 010606 plant biology & botany
Abstract: International audience; Plant dehydrins (DNHs) belong to the LEA (Late Embryogenesis Abundant) protein family and are involved in responses to multiple abiotic stresses. DHNs are classified into five subclasses according to the organization of three conserved motifs (K-; Y-; and S-segments). In the present study, the DHN protein family was characterized by molecular phylogeny, exon/intron organization, protein structure, and tissue-specificity expression in eight Fabaceae species. We identified 20 DHN genes, encompassing three (YnSKn, SKn, and K-n) subclasses sharing similar gene organization and protein structure. Two additional low conserved DHN Phi-segments specific to the legume SKn-type of proteins were also found. The in silico expression patterns of DHN genes in four legume species (Arachis duranensis, A. ipaensis, Glycine max, and Medicago truncatula) revealed that their tissue-specific regulation is associated with the presence or absence of the Y-segment. Indeed, DHN genes containing a Y-segment are mainly expressed in seeds, whereas those without the Y-segment are ubiquitously expressed. Further qRT-PCR analysis revealed that, amongst stress responsive dehydrins, a SKn-type DHN gene from A. duranensis (AdDHN1) showed opposite response to biotic and abiotic stress with a positive regulation under water deficit and negative regulation upon nematode infection. Furthermore, transgenic Arabidopsis lines overexpressing (OE) AdDHN1 displayed improved tolerance to multiple abiotic stresses (freezing and drought) but increased susceptibility to the biotrophic root-knot nematode (RKN) Meloidogyne incognita. This contradictory role of AdDHN1 in responses to abiotic and biotic stresses was further investigated by qRT-PCR analysis of transgenic plants using a set of stress-responsive genes involved in the abscisic acid (ABA) and jasmonic acid (JA) signaling pathways and suggested an involvement of DHN overexpression in these stress-signaling pathways.
Published: 2019
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29. A stress recovery signaling network for enhanced flooding tolerance in Arabidopsis thaliana

Author: Yeung, Elaine, van Veen, Hans, Vashisht, Divya, Paiva, Ana Luiza Sobral, Hummel, Maureen, Rankenberg, Tom, Steffens, Bianka, Steffen-Heins, Anja, Sauter, Margret, de Vries, Michel, Schuurink, Robert C., Bazin, Jérémie, Bailey-Serres, Julia, Voesenek, Laurentius A.C.J., Sasidharan, Rashmi, Sub Plant Ecophysiology, Plant Ecophysiology, Plant Physiology (SILS, FNWI), Sub Plant Ecophysiology, Plant Ecophysiology, Van Veen lab, Utrecht University [Utrecht], Universidade Federal do Rio Grande do Sul (UFRGS), University of California, Plant Physiology, Umeå University, Institute of Human Nutrition and Food Science, Rheinische Friedrich-Wilhelms-Universität Bonn, VU University Amsterdam, Netherlands Organisation for Scientific Research [NWO 016.VIDI.171.006, NWO-VENI 863.12.013], US National Science Foundation [MCB-1021969], US Department of Agriculture National Institute of Food and Agriculture Hatch program, Utrecht University, Vrije universiteit = Free university of Amsterdam [Amsterdam] (VU), Univ Paris Saclay, Inst Natl Rech Agron, CNRS, IPS2,Inst Plant Sci Paris Saclay, F-91405 Orsay, France, Partenaires INRAE, Bailey-Serres, Julia, Sasidharan, Rashmi, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), University of California (UC), Vrije Universiteit Amsterdam [Amsterdam] (VU), Institut des Sciences des Plantes de Paris-Saclay (IPS2 (UMR_9213 / UMR_1403)), and Université d'Évry-Val-d'Essonne (UEVE)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)
Subjects: 0106 biological sciences, 0301 basic medicine, Ribosome footprinting, [SDV]Life Sciences [q-bio], Biology, 01 natural sciences, 03 medical and health sciences, chemistry.chemical_compound, Flooding, Recovery, Gene expression, General, Abscisic acid, Gene, chemistry.chemical_classification, Abiotic component, Reactive oxygen species, Multidisciplinary, Dehydration, Abiotic stress, 15. Life on land, Cell biology, 030104 developmental biology, chemistry, 13. Climate action, Chlorophyll, flooding, ribosome footprinting, reactive oxygen species, dehydration, recovery, Homeostasis, 010606 plant biology & botany
Abstract: International audience; Abiotic stresses in plants are often transient, and the recovery phase following stress removal is critical. Flooding, a major abiotic stress that negatively impacts plant biodiversity and agriculture, is a sequential stress where tolerance is strongly dependent on viability underwater and during the postflooding period. Here we show that in Arabidopsis thaliana accessions (Bay-0 and Lp2-6), different rates of submergence recovery correlate with submergence tolerance and fecundity. A genome-wide assessment of ribosome-associated transcripts in Bay-0 and Lp2-6 revealed a signaling network regulating recovery processes. Differential recovery between the accessions was related to the activity of three genes: RESPIRATORY BURST OXIDASE HOMOLOG D, SENESCENCE-ASSOCIATED GENE113, and ORESARA1, which function in a regulatory network involving a reactive oxygen species (ROS) burst upon desubmergence and the hormones abscisic acid and ethylene. This regulatory module controls ROS homeostasis, stomata! aperture, and chlorophyll degradation during submergence recovery. This work uncovers a signaling network that regulates recovery processes following flooding to hasten the return to prestress homeostasis.
Published: 2018
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30. Use of solar radiation in the improvement of spring canola (Brassica napus L., Brassicaceae) yield influenced by nitrogen topdressing fertilization

Author: Denise Cybis Fontana, Genei Antonio Dalmago, Daniele Gutterres Pinto, Anderson Santi, Elizandro Fochesatto, Homero Bergamaschi, Jorge Alberto de Gouvêa, GENEI ANTONIO DALMAGO, CNPT, DANIELE GUTTERRES PINTO, UFRGS, DENISE CYBIS FONTANA, UFRGS, JORGE ALBERTO DE GOUVEA, CNPT, HOMERO BERGAMASCHI, UFRGS, ELIZANDRO FOCHESATTO, and ANDERSON SANTI, CNPT.
Subjects: food.ingredient, Brassica Napus, Matéria Seca, Photosynthetically active radiation, Randomized block design, Brassica, chemistry.chemical_element, Canola, Human fertilization, food, Animal science, Radiação Solar, Solar radiation, Dry matter accumulation, Dry matter, Mathematics, biology, General Medicine, biology.organism_classification, Nitrogen, Área Foliar, chemistry, Leaf area index, Interception
Abstract: The objective of the present study was to quantify and evaluate the coefficients of interception, absorption, and use of photosynthetically active solar radiation (PAR), and its effects on grain yield (YLD) of spring canola influenced by N topdressing fertilization. Two experiments were performed in 2013 and 2014 using a randomized block design, with four replicates and five treatments of N: T1-T5 (10, 20, 40, 80, and 160 kg ha -1 , respectively). Plant, PAR, and meteorological variables were measured. The lower PAR interception efficiency (ei) occurred in treatment T1. The range of light extinction coefficient (k) was of the 0.55 to 1.09. PAR absorption efficiency (ea) ranged from 86 to 93%, while the PAR use efficiency (RUE) ranging from 1.2 to to 3.7 g m -2 MJ -1 . The mean N dose for maximum agronomic efficiency (MAE) for YLD was 99.3 kg ha -1 . The YLD varied according to N treatments and was influenced by RUE, dry matter accumulated until flowering (DMF), dry matter after flowering (DMAF) and total dry matter (TDM). Nitrogen fertilization affects the balance of PAR and, consequently, the canola yield.
Published: 2018

31. Agronomic performance of soybean intercropped with cover crops and the effects of lime and Gypsum application

Author: Osvaldo Jose Ferreira Junior, E. S. O. Bortolon, Margarete Nicolodi, Leandro Bortolon, Emerson Borghi, Francelino Peteno de Camargo, Rubens Ribeiro da Silva, Clesio Gianello, OSVALDO JOSE FERREIRA JUNIOR, UFT, Gurupi-TO, LEANDRO BORTOLON, CNPASA, EMERSON BORGHI, CNPMS, ELISANDRA SOLANGE OLIVEIRA BORTOLON, CNPASA, FRANCELINO PETENO DE CAMARGO, CNPASA, RUBENS RIBEIRO DA SILVA, UFT, Gurupi-TO, MARGARETE NICOLODI, UFRGS, and CLESIO GIANELLO, UFRGS.
Subjects: 0106 biological sciences, Soja, No-tillage, Planta de Cobertura, Biology, engineering.material, 01 natural sciences, Pasture, Crop, Soil pH, Tropical pastures, Urochloa, Cover crop, Lime, geography, geography.geographical_feature_category, Glycine Max, Cover crops, fungi, food and beverages, 04 agricultural and veterinary sciences, Gypsum, biology.organism_classification, Plantio Direto, Agronomy, 040103 agronomy & agriculture, engineering, 0401 agriculture, forestry, and fisheries, Soybeans, Limes, Pennisetum, Panicum, 010606 plant biology & botany, Forrageira Tropical
Abstract: Soybean is the major crop in the Brazilian Cerrado region. Tocantins state has been increasing soybean production mostly into degraded pasture. However, cover crops such as forages crops are important to implement in regional soybean agricultural systems to increase systems resilience due to climate variability. There is a lack of information regarding to agronomic performance of soybean intercropped with cover crops under no-tillage. The experimental design was randomized complete blocks with four replications in factorial 7 × 2. Seven soybean cultivation systems were tested: 1) soybean intercropped with Urochloa brizantha cv. Marandu; 2) soybean intercropped with Urochloa ruziziensis; 3) soybean intercropped with Panicum maximum cv. Mombaça; 4) soybean intercropped with Panicum infestans cv. Massai; 5) soybean intercropped with Pennisetum americanum; 6) soybean followed by Pennisetum americanum; and 7) soybean and fallow. Two soil acidity and amelioration were tested: 1) with lime and gypsum application; 2) without lime and gypsum application. Soybean grain yield, plant height and number of pods per plant were different. Soybean grain yield were higher with lime ad gypsum application. The highest soybean plants height were observed in the treatments where lime and gypsum were applied, and with soybean intercropped with P. maximun and Millet. Soybean number of pods was positively affected P. infestans intercropped with soybean. There was no significant difference among treatments for mass per 100 seeds. Cover crops showed suitable to increase agronomic performance of soybean. Made available in DSpace on 2018-05-16T00:46:43Z (GMT). No. of bitstreams: 1 CNPASA2018jas.pdf: 194033 bytes, checksum: 930137861059086916816b0d099c41d4 (MD5) Previous issue date: 2018-05-15
Published: 2018

32. Comportamento vegetativo e produtivo de kiwizeiro ‘Elmwood’ submetido a diferentes intensidades de poda

Author: Augusto Sassi, Marília Lazarotto, Paulo Vitor Dutra de Souza, Samar Velho da Silveira, Francisco Antonello Marodin, Leonardo Zucuni Guasso, Francisco Antonello Marodin, Agronomist, Universidade Federal do Rio Grande do Sul (UFRGS) – MSc in Plant Science, Porto Alegre-RS, Brazil. E-mail: famarodin@ hotmail.com, Paulo Vítor Dutra de Souza, Professor, Dr., Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. E-mail: pvdsouza@ufrgs.br, marilia.lazarotto@ ufrgs.br., SAMAR VELHO DA SILVEIRA, CNPUV, Leonardo Zucuni Guasso, Agronomist, Universidade Federal do Rio Grande do Sul (UFRGS) – MSc in Plant Science, Porto Alegre-RS, Brazil. E-mail: famarodin@ hotmail.com, leonardoguasso@yahoo.com.br., Marília Lazarotto, Professor, Dr., Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. E-mail: pvdsouza@ufrgs.br, and Augusto Sassi, Agronomy student, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre-RS, Brazil. Email: augustobsas si@hotmail.com.
Subjects: 0106 biological sciences, productivity, Fruit weight, Plant Science, Actinidia deliciosa, lcsh:Plant culture, 01 natural sciences, lcsh:SB1-1110, Dry matter, Cane, Produção vegetal, Poda, Productivity, biology, food and beverages, 04 agricultural and veterinary sciences, kiwi fruit, biology.organism_classification, Kiwi, Produtividade, Apex (geometry), Horticulture, Shoot, 040103 agronomy & agriculture, 0401 agriculture, forestry, and fisheries, kiwi, Agronomy and Crop Science, Pruning, 010606 plant biology & botany, Food Science, Sprouting
Abstract: The objective of this study is to evaluate the vegetative and productive behavior of kiwifruit trees cv. "Elmwood" submitted to different intensities of pruning, in "Serra Gaúcha" region. Treatments consisted in maintaining different bud load levels in each cane of 10, 15 and 20 buds cane-1. The experiment, carried out during the 2016/2017 vegetative season had a completely randomized design with nine repetitions per treatment. Bud fertility, vegetative, productive and fruit quality features were evaluated. The percentage of mixed buds was higher next to the cane apex in three different treatments, while in the base of each cane ocurred the highest percentage of non sprouting buds. The decrease in the number of buds per cane induced larger leaves and increased leaf dry matter. Longitudinal and transversal diameter of fruits and fruit mass were inversely proportional to the number of buds per cane. Pruning of ?Elmwood? kiwifruit tree with different number of buds did not influence the index of real bud fertility, but affected directly leaf and shoot size. Pruning maintaining canes with 10 buds favors the increase of fruit weight and did not reduce productivity. Index terms: Actinidia deliciosa, kiwi, productivity. O objetivo deste trabalho foi avaliar o comportamento vegetativo e produtivo de kiwizeiros da cultivar "Elmwood" submetidos a diferentes intensidades de poda, na região da Serra Gaúcha. Os tratamentos utilizados consistiram na manutenção de diferentes níveis de cargas de gemas: 10; 15 e 20 gemas vara-1. O delineamento experimental foi o inteiramente casualizado, com nove repetições por tratamento. O experimento foi realizado durante o ciclo 2016/2017, avaliando-se a fertilidade das gemas, os atributos vegetativos, os produtivos e os de qualidade dos frutos. O maior percentual de brotações mistas foi próximo ao ápice da vara, nos três diferentes tratamentos; já na base dos ramos de ano, ocorreu o maior percentual de gemas que não brotaram. A diminuição da carga de gemas por vara induziu aumento do tamanho das folhas e incremento da matéria seca foliar. O diâmetro longitudinal, transversal e a massa de fruto foram inversamente proporcionais ao número de gemas por vara. A poda de kiwizeiros "Elmwood" com diferentes cargas de gemas não influencia o índice de fertilidade real das gemas, porém interfere diretamente no tamanho das folhas e dos ramos do ano. A poda, mantendo varas com 10 gemas, favorece o aumento da massa de fruto e não reduz a produtividade. Termos para indexação: Actinidia deliciosa, kiwi, produtividade. Made available in DSpace on 2018-12-23T23:29:58Z (GMT). No. of bitstreams: 1 01002945rbf406e068.pdf: 541284 bytes, checksum: e0eff7823d9a0aab2e8ac7c4df6307b1 (MD5) Previous issue date: 2018-12-20
Published: 2018

33. Genome Characterization of the First Mimiviruses of Lineage C Isolated in Brazil

Author: Felipe L. Assis, Jônatas Santos Abrahão, Fábio P. Dornas, Paulo V. M. Borato, Raíssa Nunes dos Santos, Philippe Colson, Fabrício Souza Campos, Ana Cláudia Franco, Ana Paula Moreira Franco-Luiz, Bernard La Scola, Laboratório de Vírus, Universidade Federal de Minas Gerais [Belo Horizonte] (UFMG)-Instituto de Ciências Biológicas [Goiânia, Brésil] (ICB), Departamento de Microbiologia, Imunologia e Parasitologia [Porto Alegre, Brazil], Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS)-Universidade Federal de Sao Paulo, College of Veterinary Medicine and Agronomy [Brasília, Brazil], University of Brasilia [Brazil] (UnB), Unité de Recherche sur les Maladies Infectieuses et Tropicales Emergentes (URMITE), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR48, Institut des sciences biologiques (INSB-CNRS)-Institut des sciences biologiques (INSB-CNRS)-Centre National de la Recherche Scientifique (CNRS), COMBE, Isabelle, Universidade Federal de Sao Paulo-Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), and INSB-INSB-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Microbiology (medical), Lineage (evolution), lcsh:QR1-502, Giant virus, mimivirus, Biology, Pan-genome, Microbiology, Genome, lcsh:Microbiology, 03 medical and health sciences, genomics, Mimiviridae, ORFS, Gene, Genoma, giant virus, Original Research, Synteny, Genetics, Mimivirus, [SDV.MHEP] Life Sciences [q-bio]/Human health and pathology, Phylogenetic tree, Brasil, Genomics, biology.organism_classification, 030112 virology, pan-genome, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; The family Mimiviridae, comprised by giant DNA viruses, has been increasingly studied since the isolation of the Acanthamoeba polyphaga mimivirus (APMV), in 2003. In this work, we describe the genome analysis of two new mimiviruses, each isolated from a distinct Brazilian environment. Furthermore, for the first time, we are reporting the genomic characterization of mimiviruses of group C in Brazil (Br-mimiC), where a predominance of mimiviruses from group A has been previously reported. The genomes of the Br-mimiC isolates Mimivirus gilmour (MVGM) and Mimivirus golden (MVGD) are composed of double-stranded DNA molecules of similar to 1.2 Mb, each encoding more than 1,100 open reading frames. Genome functional annotations highlighted the presence of mimivirus group C hallmark genes, such as the set of seven aminoacyl-tRNA synthetases. However, the set of tRNA encoded by the Br-mimiC was distinct from those of other group C mimiviruses. Differences could also be observed in a genome synteny analysis, which demonstrated the presence of inversions and loci translocations at both extremities of Br-mimiC genomes. Both phylogenetic and phyletic analyses corroborate previous results, undoubtedly grouping the new Brazilian isolates into mimivirus group C. Finally, an updated pan-genome analysis of genus Mimivirus was performed including all new genomes available until the present moment. This last analysis showed a slight increase in the number of clusters of orthologous groups of proteins among mimiviruses of group A, with a larger increase after addition of sequences from mimiviruses of groups B and C, as well as a plateau tendency after the inclusion of the last four mimiviruses of group C, including the Br-mimiC isolates. Future prospective studies will help us to understand the genetic diversity among mimiviruses.
Published: 2017
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34. Evaluation of a Bacillus -Based Direct-Fed Microbial on Aflatoxin B1 Toxic Effects, Performance, Immunologic Status, and Serum Biochemical Parameters in Broiler Chickens

Author: Margarita A. Arreguin, Billy M. Hargis, V. M. Petrone, Ruben Merino-Guzman, Bruno Solis-Cruz, Guillermo Tellez-Isaias, Raquel López-Arellano, Juan D. Latorre, Daniel Hernandez-Patlan, Karine Patrin Pontin, Eric Beyssac, Xochitl Hernandez-Velasco, Universidad Autónoma del Estado de México (UAEM), Universidade Federal do Rio Grande do Sul (UFRGS), University of Arkansas, Microbiologie Environnement Digestif Santé (MEDIS), INRA Clermont-Ferrand-Theix-Université Clermont Auvergne [2017-2020] (UCA [2017-2020]), Eco-Bio LLC, Arkansas Bioscience Institute, Consejo Nacional de Ciencia y Tecnologia (CONACyT) 270730, Ecosystèmes, biodiversité, évolution [Rennes] (ECOBIO), Centre National de la Recherche Scientifique (CNRS)-Observatoire des Sciences de l'Univers de Rennes (OSUR)-Institut Ecologie et Environnement (INEE), Centre National de la Recherche Scientifique (CNRS)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), and Institut National de la Recherche Agronomique (INRA)-Université Clermont Auvergne [2017-2020] (UCA [2017-2020])
Subjects: Aflatoxin, Antioxidant, 040301 veterinary sciences, [SDV]Life Sciences [q-bio], medicine.medical_treatment, Bacillus, Spleen, Biology, Feed conversion ratio, 0403 veterinary science, Animal science, Immune system, Food Animals, medicine, Bursa of Fabricius, 2. Zero hunger, broilers, General Immunology and Microbiology, business.industry, 0402 animal and dairy science, Broiler, 04 agricultural and veterinary sciences, direct-fed microbial, Poultry farming, 040201 dairy & animal science, medicine.anatomical_structure, aflatoxin B1, Animal Science and Zoology, business, performance
Abstract: International audience; The aim of the present study was to evaluate the effect of a commercial Bacillus direct-fed microbial (DFM) on aflatoxin B1 toxic effects, performance, and biochemical and immunologic parameters in broiler chickens. Ninety 1-day-old Cobb 500 male broiler chicks were raised in floor pens for a period of 21 days. Chicks were neck-tagged, individually weighed, and randomly allocated to one of three groups: Negative control (basal feed), aflatoxin B1 (basal feed + 2 ppm AFB1), and DFM (basal feed + 2 ppm AFB1 + Bacillus direct-fed microbial). Each group had three replicates of 10 chickens (n = 30/group). Body weight and body weight gain were calculated weekly, while feed intake and feed conversion ratio were determined when broilers were 21 days old. On day 21, all chickens were bled, gastrointestinal samples were collected, and spleen and bursa of Fabricius were weighed. This study confirmed that 2 ppm of AFB1 causes severe detrimental effects on performance, biochemical parameters, and immunologic parameters, generating hepatic lesions in broiler chickens (P < 0.05). However, it was also observed that DFM supplementation provided beneficial effects that might help to improve gut barrier function, anti-inflammatory and antioxidant activities, as well as humoral and cellular immunomodulation. The results of the present study suggest that this Bacillus-DFM added at a concentration of 10(6) spores/gram of feed can be used to counteract the negative effects that occur when birds consume diets contaminated with AFB1, showing beneficial effects on performance parameters, relative organ weights, hepatic lesions, immune response, and serum biochemical variables. The addition of this Bacillus-DFM might mitigate and decrease aflatoxicosis problems in the poultry industry, improving food security, alleviating public health problems, and providing economic benefits. Future studies are needed to fully elucidate the specific mechanisms by which this Bacillus-DFM counteracts the toxic effects of aflatoxin B1.
Published: 2019
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35. Italian ryegrass establishment by self-seeding in integrated crop-livestock systems: Effects of grazing management and crop rotation strategies

Author: Marcos Weber do Canto, Gilles Lemaire, Armindo Barth Neto, Paulo César de Faccio Carvalho, Anibal de Moraes, Jean Victor Savian, Radael Marinho Tres Schons, Olivier Jean François Bonnet, Universidade Federal do Paraná (UFPR), Universidade Federal do Rio Grande do Sul (UFRGS), Universidade Estadual de Maringà (UEM), Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères (P3F), Institut National de la Recherche Agronomique (INRA), European Community [266018], Brazilian National Council Scientific Development (CNPq), Coordination for the Improvement of Higher Education Personnel (CAPES), European Project: 266018,EC:FP7:KBBE,FP7-KBBE-2010-4,ANIMALCHANGE(2011), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), and Universidade de Santa Cruz do Sul (UNISC)
Subjects: Soil seed bank, [SDV]Life Sciences [q-bio], DATE, PASTURES, Soil Science, Mixed system, Tiller (botany), Plant Science, Biology, Pasture, Crop, Stocking, Grazing intensity, Grazing, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, 2. Zero hunger, geography, GREAT-PLAINS, geography.geographical_feature_category, LOLIUM-MULTIFLORUM LAM, fungi, food and beverages, 15. Life on land, Crop rotation, Seed bank persistence, Crop system, SOIL, ALLOWANCE, Self seeding, Agronomy, Stocking method, [SDE]Environmental Sciences, WEED SEED, Agronomy and Crop Science
Abstract: We evaluated the re-establishment of an Italian ryegrass pasture by self-seeding on a no-till integrated crop-livestock systems (ICLS) in the southern region of Brazil. This work is part of a long-term experimental protocol initiated in 2003. We tested the effects of various management practices, such as summer crop systems (soybean vs. maize-soybean rotation), stocking methods (continuous vs. rotational) and grazing intensities (low vs. moderate), on Italian ryegrass pasture establishment. In addition, we tested resilience of the system by testing pasture's ability to re-establish following a year without seed head production. The experiment consisted in the rotation, on the same area, of Italian ryegrass pasture grazed by sheep during the winter and up to the end of the grass production cycle, and soybean or soybean-maize grain crops rotation cultivated during the summer. The pasture established itself by self-seeding since 2005. Data were collected in 2011 and 2012 stocking season. The soybean summer crop, continuous stocking and low grazing intensity, all positively affected the production of reproductive tillers in 2011. Grazing intensity in 2011 strongly influenced early vegetative tiller densities (before crop harvest) in 2012. However, none of the grazing intensity or the stocking method treatments affected herbage mass at the end of pasture establishment in 2011 or 2012. On the other hand, the soybean summer crop positively affected pasture establishment, both in term of tiller densities and herbage mass at the end of pasture establishment. The removal of all seed heads in 2011 (preventing seed production) resulted in the total failure of pasture establishment in 2012. Overall, Italian ryegrass establishment by self-seeding relies on the annual replacement of the soil seed bank. This experiment demonstrated that under various stocking methods, moderate grazing intensity and maize or soybean summer crop, Italian ryegrass pasture establishment by self-seeding remains successful even when the stocking periods extended up to the end of the grass production cycle. Self-seeding with moderate grazing intensity ensures successful pasture establishment, reduces labour and costs and allows to increase the stocking period and so animal live weight gain over the grazing season. (C) 2013 Elsevier B.V. All rights reserved.
Published: 2014
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36. Identification of the soybean HyPRP family and specific gene response to Asian soybean rust disease

Author: Ricardo Luís Mayer Weber, Lauro Bücker Neto, Rafael Rodrigues de Oliveira, Marta Bencke, Maria Helena Bodanese Zanettini, Luciane Maria Pereira Passaglia, Caroline Cabreira, Francismar Corrêa Marcelino, Ricardo Vilela Abdelnoor, Beatriz Wiebke-Strohm, LAURO BÜCKER NETO, UFRGS, RAFAEL RODRIGUES DE OLIVEIRA, UFRGS, BEATRIZ WIEBKE-STROHM, UFRGS, MARTA BENCKE, UFRGS, RICARDO LUÍS MAYER WEBER, UFRGS, CAROLINE CABREIRA, UFRGS, RICARDO VILELA ABDELNOOR, CNPSO, FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO, MARIA HELENA BODANESE ZANETTINI, UFRGS, and LUCIANE MARIA PEREIRA PASSAGLIA, UFRGS.
Subjects: lcsh:QH426-470, Glycine max, Disease, Biology, Plant Genetics, Herdabilidade, Genome, Botany, Genetics, HyPRP genes, Molecular Biology, Gene, Abiotic component, Microarray analysis techniques, fungi, Ferrugem asiática da soja, food and beverages, Biotic stress, biology.organism_classification, real time RT-qPCR, lcsh:Genetics, Phakopsora pachyrhizi, Apis mellifera, fungal disease, Plant lipid transfer proteins, Research Article
Abstract: Soybean [Glycine max (L.) Merril], one of the most important crop species in the world, is very susceptible to abiotic and biotic stress. Soybean plants have developed a variety of molecular mechanisms that help them survive stressful conditions. Hybrid proline-rich proteins (HyPRPs) constitute a family of cell-wall proteins with a variable N-terminal domain and conserved C-terminal domain that is phylogenetically related to non-specific lipid transfer proteins. Members of the HyPRP family are involved in basic cellular processes and their expression and activity are modulated by environmental factors. In this study, microarray analysis and real time RT-qPCR were used to identify putative HyPRP genes in the soybean genome and to assess their expression in different plant tissues. Some of the genes were also analyzed by time-course real time RT-qPCR in response to infection by Phakopsora pachyrhizi, the causal agent of Asian soybean rust disease. Our findings indicate that the time of induction of a defense pathway is crucial in triggering the soybean resistance response to P. pachyrhizi. This is the first study to identify the soybean HyPRP group B family and to analyze disease-responsive GmHyPRP during infection by P. pachyrhizi.
Published: 2013
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37. The (re)discovery of ectomycorrhizal symbioses in Neotropical ecosystems sketched in Florianópolis

Author: Heidy Schimann, Altielys Casale Magnago, Andrea Rinaldi, Aída M. Vasco-Palacios, Emma Harrower, Marcelo Aloisio Sulzbacher, Marc-André Selosse, József Geml, Tine Grebenc, Francisco Kuhar, Maria Alice Neves, Lynn Delgat, Mélanie Roy, Admir José Giachini, Felipe Wartchow, Bart Buyck, Evolution et Diversité Biologique (EDB), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Fundación Biodiversa Colombia, Partenaires INRAE, Naturalis Biodiversity Center [Leiden], Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), Department of Biology, Northern Arizona University [Flagstaff], Universidade Federal de Santa Catarina = Federal University of Santa Catarina [Florianópolis] (UFSC), Slovenian Forestry Institute, University of Tennessee, Centro de Investigación y Extensión Forestal Andino Patagónico, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Department of Biomedical Sciences, University of Cagliari, Ecologie des forêts de Guyane (UMR ECOFOG), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Departamento de Solos, Universidade Federal de Santa Maria = Federal University of Santa Maria [Santa Maria, RS, Brazil] (UFSM), Universidade Federal da Paraiba (UFPB), Universidade Federal de Santa Catarina, Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal do Rio Grande do Sul (UFRGS), Université des Antilles (UA)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Naturalis Biodiversity Center, and Universidade Federal de Santa Maria (UFSM)
Subjects: 0106 biological sciences, Physiology, ectomycorrhizal fungi, [SDV]Life Sciences [q-bio], NEOTROPICAL ECOSYSTEMS, Biodiversity, Plant Science, Rainforest, Biology, 010603 evolutionary biology, 01 natural sciences, Plant Roots, ectomycorrhizal roots, Ciencias Biológicas, FRUITBODIES, Symbiosis, Fungal Diversity, Mycorrhizae, DNA Barcoding, Taxonomic, Ecosystem, ComputingMilieux_MISCELLANEOUS, biodiversity, ECTOMYCORRHIZAL FUNGI, Ecology, BARCODING, ectomycorrhizal symbioses, Community structure, 15. Life on land, Congresses as Topic, barcoding, fruitbodies, Tree (data structure), Neotropical ecosystems, ECTOMYCORRHIZAL ROOTS, 13. Climate action, BIODIVERSITY, ECTOMYCORRHIZAL SYMBIOSES, Micología, CIENCIAS NATURALES Y EXACTAS, Brazil, 010606 plant biology & botany
Abstract: The VIIIthBrazilian Mycological Congress, held in Florianopolis(Brazil) in October 2016, was organized by the UniversidadeFederal de Santa Catarina and brought together more than 1000scientists of 20 nationalities, which illustrates the dynamism ofSouth American mycology, in many fields. One of the major take-home messages, amongst many research talks and posters, was therising interest in the ectomycorrhizal (EM) symbiosis, which wasaddressedbybothasymposium anda3-day workshop ?Totherootsof trees: growing awareness of ectomycorrhizal symbioses in theNeotropics?. Talks on this symbiosis were given by taxonomists andecologists, and focused on the Neotropics, the tropical realm thatextends from the central plateau of Mexico and which includesSouth America. The inventories of ectomycorrhizal fungi (EMF),observations of EM roots, and metabarcoding studies presentedrevealed a hitherto unsuspected abundance of the EM symbiosis toall participants, including a number of South-American students.This meeting provided great opportunities to share points of viewon the historical reasons behind gaps in knowledge on EMFassociations in neotropical low-elevation areas. The meeting alsohighlighted recent discoveries and forthcoming challenges, basedon three complementary approaches: studies on fruitbodies, EMroots, and the metabarcoding of soil samples. Fil: Roy, Mélanie. Universite Paul Sabatier Toulouse III; Francia Fil: Vasco-palacios, Aida. Universidad de Antioquia; Colombia. Fundación Biodiversa Colombia; Colombia Fil: Geml, József. Naturalis Biodiversity Center; Países Bajos Fil: Buyck, Bart. Museum National D; Francia Fil: Delgat, Lynn. University Of Ghent; Bélgica Fil: Giachini, Admir. Universidade Federal Da Santa Catarina; Brasil Fil: Grebenc, Tine. Slovenian Forestry Institute; Eslovenia Fil: Harrower, Emma. University Of Tennessee; Estados Unidos Fil: Kuhar, José Francisco. Centro de Investigación y Extensión Forestal Andino Patagónico; Argentina Fil: Magnago, Altielys. Universidade Federal Do Rio Grande Do Sul; Brasil Fil: Rinaldi, Andrea C.. Universita Degli Studi Di Cagliari; Italia Fil: Schimann, Heidy. Universite Des Antilles Et de la Guyane; Guyana Fil: Selosse, Marc-andré. Museum National D; Francia Fil: Sulzbacher, Marcelo Aloisio. Universidade Federal de Santa Maria; Brasil Fil: Wartchow, Felipe. Universidade Federal da Paraíba; Brasil Fil: Neves, Maria Alice. Universidade Federal Da Santa Catarina; Brasil
Published: 2017
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38. Canine leproid granuloma: Study of 27 cases

Author: Fabiana Wurster, Alexandre Secorun Borges, Saulo Petinatti Pavarini, Luciana Sonne, Daniele Mariath Bassuino, David Driemeier, Gustavo S. Silva, José P. Oliveira-Filho, Universidade Federal do Rio Grande do Sul (UFRGS), UFRGS, and Universidade Estadual Paulista (Unesp)
Subjects: lcsh:Veterinary medicine, Bacteriologia, General Veterinary, Caes [Patologia veterinaria], Dermatologia veterinaria, Histology, achados patológicos, doenças cutâneas, Mycobacterium sp, Canine leproid granuloma, Biology, Molecular biology, Cutaneous diseases, Granuloma leproide canino, Micologia, lcsh:SF600-1100, Dermatopatias infecciosas, Pathological findings
Abstract: RESUMO: O granuloma leproide canino (GLC) é uma doença micobacteriana que cursa com lesão nodular, cutânea ou subcutânea, tipicamente auto limitante, decorrente de infecção pelo Mycobacterium. É uma doença dermatológica rara, usualmente relatada em países de clima tropical. O objetivo deste trabalho é caracterizar as alterações microscópicas e epidemiológicas de casos de GLC. Além disso, utilizar exames complementares para demonstração do Mycobacterium sp. na lesão analisada utilizando coloração de Ziehl-Neelsen, imuno-histoquímica e PCR para classificação e sequenciamento da espécie de Mycobacterium. Foram analisados 27 casos de GLC diagnosticados no período compreendido de 2005 a 2014 (21 exames histológicos e seis citológicos). A raça mais acometida foi Boxer (33,3%), seguida da Dachshund (11,1%). Foi frequente o acometimento de caninos de grande porte (64%) e de pelame curto (96%). Não foi possível estabelecer qualquer padrão de sazonalidade do GLC. As lesões se localizaram anatomicamente no pavilhão auricular na maioria dos casos (93%), constituindo-se de nódulos únicos ou múltiplos, ulcerados ou não. Em nenhum dos casos foi observado acometimento sistêmico. Lesões histológicas seguiram três padrões que variaram de nodulares a difusos, com infiltrado granulomatoso/piogranulomatoso com envolvimento de derme superficial e profunda. A observação de bacilos pela coloração de Ziehl-Neelsen confirmou o diagnóstico. Este teste demonstrou grande variação na quantidade de bacilos nas lesões, não relacionado estatisticamente ao tipo da lesão nem ao período evolutivo. A imuno-histoquímica foi positiva em 90% dos casos, e em alguns casos facilitou a identificação de bacilos, entretanto, em dois casos ocorreram resultados negativos mesmo havendo marcação pela coloração de Ziehl-Neelsen. Na técnica de PCR realizada em 18 amostras, 44% dos casos foram positivos, duas sequências inespecíficas, e demonstrou ainda, que Mycobacterium murphy foi responsável por 2 casos e Mycobacterium hodleri em um caso. Em outros três casos não se obteve 100% de semelhança entre o produto sequenciado e os demais Mycobacterium. ABSTRACT: Canine leproid granuloma (CLG) is cutaneous diseases caused by mycobacteria that course with nodular cutaneous or subcutaneous lesion, typically self-limiting, due to Mycobacterium infection. It is an unusual dermatological disease, usually reported in tropical countries. The objective of the present study is to describe the epidemiological and histopathological findings of CLG. Furthermore, use additional tests for demonstration of Mycobacterium sp. in the lesion were performed, e.g. Ziehl-Neelsen staining, immunohistochemical and polymerase chain reaction (PCR) for classification and genetic sequencing of Mycobacterium species. Twenty seven cases of CLG diagnosed between 2005 and 2014 were studied (21 cases by histological examination and six by cytological examination). Boxer dogs were the breed most affected (33.3%) followed by Dachshund dog breed (11.1%). The disease affected dogs with mainly large size (64%) and short-coated (96%). There was no evidence predilection for occurrence in any season. The lesions were localized, in most cases, at the pinna (93%), comprise one or multiple nodules, ulcerated or not. None of the cases was observed with systemic involvement. Histopathological findings included three patterns of nodular to diffuse, with inflammatory infiltration granulomatous/pyogranulomatous that involved superficial and deep dermis. Definitive diagnosis was made by the presence of acid-fast bacilli by Ziehl-Neelsen stain, showing wide variation in the number of bacilli in the lesions, not being statistically correlated to the type of injury or the evolution period. Immunohistochemistry was effective, and in some cases facilitated the identification of bacilli, but in two cases were negative even with marking by Ziehl-Neelsen staining. The immunohistochemistry was positive in 90% cases, and in some cases facilitated the identification of bacilli, but in two cases were negative even with marking by Ziehl-Neelsen staining. The PCR technique was positive in 44% of cases, demonstrating that Mycobacterium murphy was responsible for two cases of GLC and Mycobacterium hodleri by a one case. In other three PCR-positive cases if not got 100% similarity between the sequenced and other Mycobacterium.
Published: 2017

39. Landscape genomic approach to detect selection signatures in locally adapted Brazilian swine genetic groups

Author: Jaime Araujo Cobuci, Robson Jose Cesconeto, Stéphane Joost, José Braccini, Samuel Rezende Paiva, Concepta McManus, ROBSON JOSE CESCONETO, UFRGS, STÉPHANE JOOST, ECOLE POLYTECHNIQUE FÉDÉRALE DE LAUSANNE (EPFL), SWITZERLAND, CONCEPTA MARGARET MCMANUS, UNB, SAMUEL REZENDE PAIVA, Cenargen, JAIME ARAUJO COBUCI, UFRGS, and JOSE BRACCINI, UFRGS.
Subjects: 0301 basic medicine, Conservation genetics, Germplasm, molecular markers, Range (biology), Sus scrofa, Biology, Population structure, 03 medical and health sciences, Gene bank, Animal genetic resources, Genotype, Allele, Ecology, Evolution, Behavior and Systematics, Selection (genetic algorithm), Nature and Landscape Conservation, Original Research, Genetics, Ecology, population structure, animal genetic resources, Breed, 030104 developmental biology, conservation genetics, Molecular markers
Abstract: Samples of 191 animals from 18 different Brazilian locally adapted swine genetic groups were genotyped using Illumina Porcine SNP60 BeadChip in order to identify selection signatures related to the monthly variation of Brazilian environmental variables. Using BayeScan software, 71 SNP markers were identified as FST outliers and 60 genotypes (58 markers) were found by Samβada software in 371 logistic models correlated with 112 environmental variables. Five markers were identified in both methods, with a Kappa value of 0.073 (95% CI: 0.011–0.134). The frequency of these markers indicated a clear north–south country division that reflects Brazilian environmental differences in temperature, solar radiation, and precipitation. Global spatial territory correlation for environmental variables corroborates this finding (average Moran's I = 0.89, range from 0.55 to 0.97). The distribution of alleles over the territory was not strongly correlated with the breed/genetic groups. These results are congruent with previous mtDNA studies and should be used to direct germplasm collection for the National gene bank.
Published: 2017

40. Insights on the virulence of swine respiratory tract mycoplasmas through genome-scale metabolic modeling

Author: Franciele Maboni Siqueira, Florence Tardy, Mariana Galvão Ferrarini, Ana Tereza Ribeiro de Vasconcelos, Marie-France Sagot, Arnaldo Zaha, Bénédicte Elena-Herrmann, Elodie Jobard, Tony Lionel Palama, Scheila G. Mucha, Irene Silveira Schrank, Equipe de recherche européenne en algorithmique et biologie formelle et expérimentale (ERABLE), Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria), Centro de Biotecnologia [Porto Alegre] (CBiot), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Centre National de la Recherche Scientifique (CNRS)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de la Recherche Agronomique (INRA), Centre Léon Bérard [Lyon], NMR Methods for Metabolism - Methodes RMN en métabolomique, Institut des Sciences Analytiques (ISA), Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon)-Centre National de la Recherche Scientifique (CNRS)-Université de Lyon-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-École normale supérieure - Lyon (ENS Lyon), Laboratorio Nacional de Computação Cientifica [Rio de Janeiro] (LNCC / MCT), Mycoplasmoses des Ruminants - UMR (MYCO), Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Baobab, Département PEGASE [LBBE] (PEGASE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Equipe de recherche européenne en algorithmique et biologie formelle et expérimentale ( ERABLE ), Institut National de Recherche en Informatique et en Automatique ( Inria ) -Institut National de Recherche en Informatique et en Automatique ( Inria ), Centro de Biotecnologia [Porto Alegre] ( CBiot ), Universidade Federal do Rio Grande do Sul [Porto Alegre] ( UFRGS ), Laboratoire de Biométrie et Biologie Evolutive ( LBBE ), Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique ( Inria ) -Centre National de la Recherche Scientifique ( CNRS ), Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés ( LISBP ), Institut National de la Recherche Agronomique ( INRA ) -Institut National des Sciences Appliquées - Toulouse ( INSA Toulouse ), Institut National des Sciences Appliquées ( INSA ) -Institut National des Sciences Appliquées ( INSA ) -Centre National de la Recherche Scientifique ( CNRS ), Institut des Sciences Analytiques ( ISA ), École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ) -École normale supérieure - Lyon ( ENS Lyon ) -Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique ( CNRS ), Laboratorio Nacional de Computação Cientifica [Rio de Janeiro] ( LNCC / MCT ), Mycoplasmoses des ruminants [Lyon], Université de Lyon-VetAgro Sup ( VAS ), VetAgro Sup ( VAS ), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS), ISA NMR Methods for Metabolism - Methodes RMN en métabolomique (2014-2018), Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS)-Institut de Chimie du CNRS (INC)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), and Université de Lyon-Université de Lyon-Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, Magnetic Resonance Spectroscopy, Swine, Whole-genome metabolic reconstruction, Mycoplasma hyorhinis, [SDV]Life Sciences [q-bio], 030106 microbiology, Mollicutes, Virulence, Biology, medicine.disease_cause, Models, Biological, Microbiology, 03 medical and health sciences, Mycoplasma, Mycoplasma hyopneumoniae, Genetics, medicine, Animals, Metabolomics, Biomass, Microbial Viability, [ SDV ] Life Sciences [q-bio], Computational Biology, Metabolic network, Genomics, Pneumonia of Swine, Mycoplasmal, biology.organism_classification, medicine.disease, Hydrogen peroxide, Virology, Bacterial Load, Gene Ontology, medicine.anatomical_structure, Metabolism, Enzootic, Energy Metabolism, Pneumonia (non-human), Genome, Bacterial, Metabolic Networks and Pathways, Research Article, Biotechnology, Respiratory tract
Abstract: Background The respiratory tract of swine is colonized by several bacteria among which are three Mycoplasma species: Mycoplasma flocculare, Mycoplasma hyopneumoniae and Mycoplasma hyorhinis. While colonization by M. flocculare is virtually asymptomatic, M. hyopneumoniae is the causative agent of enzootic pneumonia and M. hyorhinis is present in cases of pneumonia, polyserositis and arthritis. The genomic resemblance among these three Mycoplasma species combined with their different levels of pathogenicity is an indication that they have unknown mechanisms of virulence and differential expression, as for most mycoplasmas. Methods In this work, we performed whole-genome metabolic network reconstructions for these three mycoplasmas. Cultivation tests and metabolomic experiments through nuclear magnetic resonance spectroscopy (NMR) were also performed to acquire experimental data and further refine the models reconstructed in silico. Results Even though the refined models have similar metabolic capabilities, interesting differences include a wider range of carbohydrate uptake in M. hyorhinis, which in turn may also explain why this species is a widely contaminant in cell cultures. In addition, the myo-inositol catabolism is exclusive to M. hyopneumoniae and may be an important trait for virulence. However, the most important difference seems to be related to glycerol conversion to dihydroxyacetone-phosphate, which produces toxic hydrogen peroxide. This activity, missing only in M. flocculare, may be directly involved in cytotoxicity, as already described for two lung pathogenic mycoplasmas, namely Mycoplasma pneumoniae in human and Mycoplasma mycoides subsp. mycoides in ruminants. Metabolomic data suggest that even though these mycoplasmas are extremely similar in terms of genome and metabolism, distinct products and reaction rates may be the result of differential expression throughout the species. Conclusions We were able to infer from the reconstructed networks that the lack of pathogenicity of M. flocculare if compared to the highly pathogenic M. hyopneumoniae may be related to its incapacity to produce cytotoxic hydrogen peroxide. Moreover, the ability of M. hyorhinis to grow in diverse sites and even in different hosts may be a reflection of its enhanced and wider carbohydrate uptake. Altogether, the metabolic differences highlighted in silico and in vitro provide important insights to the different levels of pathogenicity observed in each of the studied species. Electronic supplementary material The online version of this article (doi:10.1186/s12864-016-2644-z) contains supplementary material, which is available to authorized users.
Published: 2016
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41. Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

Author: Fenton P.D. Cotterill, Fernanda P. Werneck, Stephen W. Chordas, Enrique González-Soriano, Pierangelo Luporini, Santiago Claramunt, Santosh Kumar, Adriano B. Kury, Marcelo José Sturaro, Atsushi Tominaga, Marcos Gonçalves Lhano, Giulio Cuccodoro, Bernardo F. Santos, Alejandro Oceguera-Figueroa, Klaus Henle, Giovanni B. Delmastro, Thibaut Delsinne, Jeremy A. Miller, Thomas Ziegler, Ishan Agarwal, Rodrigo M. Feitosa, Robert C. Glotzhober, Giuliano Doria, Adeline Soulier-Perkins, Diego Baldo, Valéria da Cunha Tavares, Danilo Pacheco Cordeiro, Eli Greenbaum, Carlos Alberto Santos de Lucena, Stuart V. Nielsen, Jörn Köhler, Fernando Pacheco Rodrigues, Justin C. Bagley, Shun Ichiro Naomi, Gustavo Hormiga, Geoffrey Odhiambo Ong'ondo, Aurélien Miralles, Alexandre Uarth Christoff, Florian M. Steiner, Matthias Glaubrecht, Victor Van Cakenberghe, Wolfgang Rabitsch, Jack W. Sites, Norma J. Salcedo, Mario Alberto Cozzuol, Ward C. Wheeler, Krister T. Smith, Brian Tilston Smith, Ignacio Jose De La Riva De La Viña, Leo J. Borkin, Ângelo Parise Pinto, Marivene R. Manuel-Santos, Ana Carolina Pavan, M. J. Alves, Dan Cogălniceanu, Luciana F. Santoferrara, James M. Carpenter, Thierry Deuve De Resbecq, Beat Schätti, Jean Pierre Vacher, John G. Day, Ray C. Schmidt, Otto M. P. Oliveira, Lázaro Guevara, Jean-Lou Justine, Karthikeyan Vasudevan, Donat Agosti, Cécile Mourer-Chauviré, Brett C. Ratcliffe, Birgit C. Schlick-Steiner, Sebastian Kvist, Nathan K. Lujan, Robert Alexander Pyron, Rosana M. Rocha, Roberto Poggi, José A. Langone, Larry Lee Grismer, Václav Gvoždík, Natsuhiko Yoshikawa, Thaís P. Miranda, Elizabeth Prendini, Abel Pérez-González, Katharina C. Wollenberg Valero, Jean-Yves Rasplus, Cristiano R. Moreira, Antonietta La Terza, Fabio Siqueira Pitaluga de Godoi, Michael W. Holmes, Thomas E. Lacher, Ronald H. Pine, Matthew P. Heinicke, Steven M. Goodman, John D. Lynch, Elöd Kondorosy, Anderson Feijó, Orfeo Picariello, Wolfgang Denzer, Stefano Valdesalici, Aléssio Datovo, Jean Pierre Hugot, Yuri L. R. Leite, Heinz Grillitsch, Hernán Ortega, Dimitri Forero, Jean Carlos Santos, Marie Claude Durette-Desset, Victor H. Gonzalez, Mrugank Prabhu, Walter E. Schargel, Beate Röll, Caleb D. McMahan, Mitsuru Kuramoto, Edson A. Adriano, Jérôme Constant, Richard Laval, María A. Mendoza-Becerril, Cédric d'Udekem d'Acoz, Alain Didier Missoup, Frank Tillack, Janet K. Braun, Lindsey Swierk, André L. Netto-Ferreira, Xiaofeng Lin, Karl Heinz Jungfer, Fabio Di Dario, Vanessa Kruth Verdade, Pavel Štys, Franco Andreone, Andrés A. Ojanguren-Affilastro, Manuel Ruedi, Didier Van den Spiegel, Rahul Khot, Lars Krogmann, Lance Grande, Robert C. Drewes, Luis M. P. Ceríaco, Jeffrey W. Streicher, Jacob A. Esselstyn, Josiah H. Townsend, Wolfgang Arthofer, Wiesław Bogdanowicz, Marcos A. Raposo, Omar Torres-Carvajal, Dirk Ahrens, Theo Blick, Carlos DoNascimiento, Eric Drouet, Claudia Patricia Ornelas-García, Gervásio Silva Carvalho, Zachary H. Falin, Gaetano Odierna, Michael Maia Mincarone, Sabine Agatha, Christian De Muizon, Célio F. B. Haddad, Pablo Rodrigues Gonçalves, Maarten P.M. Vanhove, Ronald Janssen, Ulrich Burkhardt, Bernard Landry, Paúl M. Velazco, Melanie L. J. Stiassny, Erna Aescht, Sarah Siqueira Oliveira, Koshiro Eto, Thomas van de Kamp, Fabio Cianferoni, Leonardo Ferreira Machado, Luiz Carlos Pinho, Dennis Rödder, Fábio Raposo do Amaral, Shan Gao, Paulo Passos, Nikolai L. Orlov, Emanuel Tschopp, Bert Van Bocxlaer, Roman Hołyński, Isabella Van De Velde, Indraneil Das, Luciano Damián Patitucci, Daniel J. Bennett, Annemarie Ohler, Rachunliu G. Kamei, Patrick Grootaert, Tony Robillard, Jun Gong, Massimo Delfino, Antonio C. Marques, Daizy Bharti, Ira Richling, José L. O. Birindelli, Thiago Borges Fernandes Semedo, Philippe Grandcolas, Eric J. Sargis, Andreas Taeger, Jesús Molinari, Link E. Olson, Christoph Kucharzewski, Luc Janssens de Bisthoven, José P. Pombal, Ryan C. McKellar, Serge Gofas, Mário C. C. de Pinna, Kristofer M. Helgen, Pablo Quintela-Alonso, Marcos Tavares, Wolfgang A. Nässig, Jodi J. L. Rowley, Jairo Arroyave, Fabio Maria Guarino, Djoko T. Iskandar, Martin Fikáček, Joel Cracraft, Robert M. Timm, Lassad Neifar, Marcelo C. Andrade, Moisés Escalona, Max Kieckbusch, George R. Zug, J. V. Remsen, Weibo Song, Paula Beatriz Araujo, Marco Brandalise de Andrade, Luiz Alexandre Campos, Eva V. Bärmann, Thomas Lehmann, Thorsten Stoeck, Jorge Salazar-Bravo, Charles Morphy D. Santos, Joël Minet, Mann Kyoon Shin, Gustavo A. Bravo, Felipe Franco Curcio, Antoine Pariselle, Hidetoshi Ota, David R. Luz, Abdulaziz S. Alqarni, Joseph A. Cook, Cameron D. Siler, Zilda Margarete Seixas de Lucena, Guarino R. Colli, Máriom A. Carvajal, Franziska Bauer, Yves Samyn, Luke Tornabene, Stefan Merker, Favízia Freitas de Oliveira, Murilo N. L. Pastana, Luís Fábio Silveira, Moira Jane FitzPatrick, Stephen D. Busack, Max R. Lambert, Julián Faivovich, Masafumi Matsui, Bernhard A. Huber, Alexandre Aleixo, Mariana P. Marques, Jean-François Trape, Marcello Guimarães Simões, Brian L. Fisher, Brandi S. Coyner, Michael F. Bates, Marcelo Salles Rocha, Silke Schweiger, Jean Raffaëlli, Vladimir Dinets, Paulo C. A. Garcia, Devanshu Gupta, Juan M. Guayasamin, W. Brian Simison, Rudy Jocqué, Aniruddha Datta-Roy, Marcelo R. Britto, Cristiane Bastos-Silveira, Celso O. Azevedo, Roger Bour, Aidin Niamir, Leandro M. Vieira, Mark Epstein, Neal Woodman, Marcelo R. de Carvalho, José Antonio González-Orej, Martin Kruger, Ulisses Caramaschi, Marcus Guidoti, Cibele Biondo, Scott Lyell Gardner, François Dusoulier, Francisco Langeani, John E. Lattke, Helen M. Barber-James, Jan Zima, Guilherme R. R. Brito, Ricardo Moratelli, Stylianos Chatzimanolis, Carlos José Einicker Lamas, John B. Iverson, Maria Hołyńska, Aaron M. Bauer, Luc Brendonck, Klaus-Peter Koepfli, Angelica Crottini, Cristian Hernan Fulvio Perez, Tiago Georg Pikart, Eliécer E. Gutiérrez, Luis García-Prieto, Lawrence R. Heaney, Thomas A. Munroe, Thomas C. Giarla, Laurie J. Vitt, Enrico Borgo, Antonio J. C. Aguiar, Sven O. Kullander, Jean Sébastien Steyer, Marcial Quiroga-Carmona, Matthew J. Miller, Kraig Adler, Werner Conradie, Enrique La Marca, Thomas Schmitt, Dieter Uhl, Mario de Vivo, Rainer Hutterer, Silvio Shigueo Nihei, Perry L. Wood, Amira Chaabane, Tim Tokaryk, Octávio Mateus, Andrés Sebastián Quinteros, Daniel S. Fernandes, Alexandra Cartaxana, Pedro F. Victoriano, Ernest C.J. Seamark, William R. Branch, Mark-Oliver Rödel, Diego Astúa, Marcio R. Pie, Julien Pétillon, Henrard Arnaud, Hossein Rajaei, Sushil K. Dutta, Hussam Zaher, Hernández Díaz Yoalli Quetzalli, Martin Carr, Renan Carrenho, Estefanía Rodríguez, Robert Trusch, Patrick David, Rafaela Lopes Falaschi, Rafael O. de Sá, Miguel Ângelo Marini, Varad B. Giri, Jean-Claude Rage, Guilherme S. T. Garbino, Björn Berning, Thierry Frétey, Vítor de Q. Piacentini, Paulo A. Buckup, David C. Lees, Alfred L. Gardner, Marco Pavia, Pablo Ricardo Mulieri, Lorenzo Prendini, Eliana M. Cancello, Cinthia Chagas, Bruce B. Collette, Leigh R. Richards, Eduardo I. Faúndez, Timothy J. Colston, Thomas Keith Philips, Miguel Trefaut Rodrigues, Renato Gregorin, Karin Meißner, Nathan S. Upham, A. Townsend Peterson, Tiago Kütter Krolow, Felipe Ferraz Figueiredo Moreira, Olivier Montreuil, Leandro M. Sousa, Thomas Weisse, Natalia B. Ananjeva, Donald C. Taphorn, Renata Stopiglia, Marcelo Duarte, Benoit Guénard, Cyril Gallut, Giovanni Boano, David Modrý, Erik Verheyen, Jonas José Mendes Aguiar, Sven Mecke, Alexandre Hassanin, Robert M. Zink, Marcello Mezzasalma, André Silva Roza, Reginaldo Constantino, Alice Hirschmann, Ulisses Pinheiro, Edmundo González-Santillán, Carlos A. Mendoza-Palmero, Tom Artois, Fernando J. M. Rojas-Runjaic, Kailas Chandra, Pablo Teta, Michael Karner, Esteban O. Lavilla, Mauricio Ortega-Andrade, Alexandra Marçal Correia, Deepak Veerappan, Daniela M. Takiya, Bolívar R. Garcete-Barrett, Alexander Kupfer, Sérgio N. Stampar, Daniel Burckhardt, Michael S. Engel, Teresa Kearney, Silvia E. Pavan, Luiz Roberto Malabarba, Mark D. Scherz, Pedro L. V. Peloso, Christiane Denys, Matthias F. Geiger, Alexander Pelzer, Jose G. Tello, Fabio S. Nascimento, Juan D. Daza, Franger J. García, Cinthia A. Brasileiro, Martín J. Ramírez, Marcos Pérsio Dantas Santos, Twan A. A. M. Leenders, Alain Canard, Tomáš Mazuch, Axel Hausmann, Flávio Alicino Bockmann, Prosanta Chakrabarty, Jasmine Purushothaman, Ara Monadjem, David A. Donoso, Kaushik Deuti, Stephen Mahony, Duke S. Rogers, Don E. Wilson, Julian C. Kerbis Peterhans, Jader Marinho-Filho, Alain Dubois, Marcio Luiz de Oliveira, Jan Decher, John M. Midgley, Fernando C. Jerep, Bastian Bentlage, Ivan Löbl, Gregory J. Watkins-Colwell, Uwe Fritz, Annamaria Nistri, Lúcia H. Rapp Py-Daniel, Bruce D. Patterson, Peter J. Taylor, Burton K. Lim, James L. Patton, Colin S. Schoeman, Stéphane Grosjean, Ismael Franz, Cristian Simón Abdala, John S. Sparks, Marcos R. Bornschein, Leonora Pires Costa, Martín O. Pereyra, João Filipe Riva Tonini, Richard Schodde, Blanca Pérez-Luz, Cristiano Feldens Schwertner, Peter Jäger, Marcin Jan Kamiński, Philipp Wagner, Jakob Hallermann, Hendrik Freitag, Olavi Kurina, Laure Desutter-Grandcolas, Romain Garrouste, Pedro De Podestà Uchôa de Aquino, Guillermo D’Elía, Sharlene E. Santana, Roberto E. Reis, Wouter Dekoninck, Sushma Reddy, Alfred L. Rosenberger, James R. McCranie, Wolfgang Böhme, Ricardo C. Benine, Cyrille D'Haese, Paulo H. F. Lucinda, Jacques H. C. Delabie, Carr, Martin, Department of Biology, Northern Arizona University [Flagstaff], Museu Nacional de Historia Natural e da Ciencia, Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade de Brasilia [Brasília] (UnB), National Museum of Natural History, National Museum of Natural History - Leiden, Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Pierre et Marie Curie (Paris 6), Centre National de la Recherche Scientifique (CNRS), Sorbonne Universités, Universidad Nacional de Tucumán (UNT), King Saud University, Cornell University, Universidade Federal de São Paulo, Austrian Museum, Villanova University, Universität Salzburg, Plazi, University of São Paulo, Zoologisches Forschungsmuseum Alexander Koenig, Museu Paraense Emílio Goeldi, Russian Academy of Sciences, Federal University of Para - Universidade Federal do Para [Belem - Brésil], Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS), Museo Regionale di Scienze Naturali, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Royal Museum for Central Africa [Tervuren] (RMCA), Universidad Nacional Autónoma de México (UNAM), Institute of Ecology, Technische Universität Berlin (TUB), Hasselt University, Universidade Federal de Pernambuco [Recife] (UFPE), Universidade Federal do Espírito Santo (UFES), Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie]), Albany Museum, National Museum, Senckenberg Naturhistorische Sammlungen, Universidade Estadual Paulista Júlio de Mesquita Filho [São José do Rio Preto] (UNESP), Stephen F. Austin State University, Smithsonian Institution, Tyrolean State Museum, Università di Camerino, Universidade Federal do ABC, Museu de Zoologia da Universidade Estadual de Londrina, Senckenberg Research Institute, Museo Civico di Storia Naturale, Muzeum i Instytut Zoologii Polskiej Akademii Nauk, Russian Academy of Sciences [Moscow] (RAS), Port Elizabeth Museum, Sam Noble Museum, Harvard University [Cambridge], North West University, Museu Nacional do Rio de Janeiro, Musée d'Histoire Naturelle de Bâle, Senckenberg Museum [Frankfurt], North Carolina Museum of Natural Sciences, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Museu de Zoologia (MZ), Universidade de São Paulo (USP), American Museum of Natural History, University of Huddersfield, North Dakota State University (NDSU), Faculté des Sciences de Sfax, Université de Sfax - University of Sfax, Departamento de Polícia Técnico Científica (DPTC), Museum of Natural Science, Louisiana State University (LSU), Zoological Survey of India, University of Tennessee System, Ohio State University [Columbus] (OSU), Museu de Ciências Naturais, Universidade Luterana do Brasil (ULBRA), Museo di Storia Naturale, Università degli Studi di Firenze, Ovidius University of Constanta, The University of Mississippi [Oxford], Royal Belgian Institute of Natural Sciences (RBINS), The University of New Mexico [Albuquerque], Instituto Nacional de Pesquisas da Amazônia (INPA), University of Stellenbosh, Universidade Federal de Minas Gerais, Centro de Investigaçao em Biodiversidade e Recursos Genéticos, Museum d'Histoire Naturelle, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL), Université Pierre et Marie Curie - Paris 6 (UPMC), Sorbonne Université (SU), King Saud University [Riyadh] (KSU), Cornell University [New York], Villanova University [USA], Universidade de São Paulo = University of São Paulo (USP), Museu Paraense Emílio Goeldi [Belém, Brésil] (MPEG), the Russian Academy of Sciences [Moscow, Russia] (RAS), Federal University of Para - Universidade Federal do Pará - UFPA [Belém, Brazil] (UFPA), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), Technical University of Berlin / Technische Universität Berlin (TU), Hasselt University (UHasselt), Universidade Federal do Espirito Santo (UFES), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Università degli Studi di Camerino = University of Camerino (UNICAM), Harvard University, North-West University [Potchefstroom] (NWU), Université de Rennes (UR), American Museum of Natural History (AMNH), Museo di Storia Naturale di Firenze, Università degli Studi di Firenze = University of Florence (UniFI), Stellenbosch University, Museum d'Histoire Naturelle [Genève] (MHN), Ceríaco, Luis M. P., Gutiérrez, Eliécer E., Dubois, Alain, Abdala, Cristian Simón, Alqarni, Abdulaziz S., Adler, Kraig, Adriano, Edson A., Aescht, Erna, Agarwal, Ishan, Agatha, Sabine, Agosti, Donat, Aguiar, Antonio J. C., Aguiar, Jonas José Mende, Ahrens, Dirk, Aleixo, Alexandre, Alves, Maria Judite, Do Amaral, Fabio Raposo, Ananjeva, Natalia, Andrade, Marcelo C., De Andrade, Marco Brandalise, Andreone, Franco, Aquino, Pedro P. U., Araujo, Paula Beatriz, Arnaud, Henrard, Arroyave, Jairo, Arthofer, Wolfgang, Artois, Tom J., Astúa, Diego, Azevedo, Celso, Bagley, Justin C., Baldo, Diego, Barber James, Helen Margaret, Bärmann, Eva V., Bastos Silveira, Cristiane, Bates, Michael F., Bauer, Aaron M., Bauer, Franziska, Benine, Ricardo C., Bennett, Daniel J., Bentlage, Bastian, Berning, Björn, Bharti, Daizy, Biondo, Cibele, Birindelli, José, Blick, Theo, Boano, Giovanni, Bockmann, Flávio A., Bogdanowicz, Wieslaw, Böhme, Wolfgang, Borgo, Enrico, Borkin, Leo, Bornschein, Marcos Ricardo, Bour, Roger, Branch, William R., Brasileiro, Cinthia A., Braun, Janet K., Bravo, Gustavo A., Brendonck, Luc, Brito, Guilherme R. R., Britto, Marcelo R., Buckup, Paulo A., Burckhardt, Daniel, Burkhardt, Ulrich, Busack, Stephen D., Campos, Luiz A., Canard, Alain, Cancello, Eliana M., Caramaschi, Ulisse, Carpenter, James M., Carrenho, Renan, Cartaxana, Alexandra, Carvajal, Mariom A., Carvalho, Gervásio Silva, De Carvalho, Marcelo Rodrigue, Chaabane, Amira, Chagas, Cinthia, Chakrabarty, Prosanta, Chandra, Kaila, Chatzimanolis, Styliano, Chordas, Stephen W., Christoff, Alexandre U., Cianferoni, Fabio, Claramunt, Santiago, Cogãlniceanu, Dan, Collette, Bruce B., Colli, Guarino R., Colston, Timothy J., Conradie, Werner, Constant, Jérôme, Constantino, Reginaldo, Cook, Joseph A., Cordeiro, Danilo, Correia, Alexandra Marçal, Cotterill, Fenton P. D., Coyner, Brandi, Cozzuol, Mario A., Cracraft, Joel, Crottini, Angelica, Cuccodoro, Giulio, Curcio, Felipe Franco, D'Udekem D'Acoz, Cédric, D'Elía, Guillermo, D'Haese, Cyrille, Das, Indraneil, Datovo, Aléssio, Datta Roy, Aniruddha, David, Patrick, Day, John G., Daza, Juan D., De Bisthoven, Luc Janssen, De La Riva De La Viña, Ignacio Jose, De Muizon, Christian, De Pinna, Mario, Piacentini, Vítor De Q., De Sá, Rafael O., De Vivo, Mario, Decher, Jan, Dekoninck, Wouter, Delabie, Jacques H. 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A., Gardner, Alfred L., Gardner, Scott Lyell, Garrouste, Romain, Geiger, Matthias F., Giarla, Thomas C., Giri, Varad, Glaubrecht, Matthia, Glotzhober, Robert C., Godoi, Fabio S. P., Gofas, Serge, Gonçalves, Pablo R., Gong, Jun, Gonzalez, Victor H., González Orej, José Antonio, González Santillán, Edmundo, González Soriano, Enrique, Goodman, Steven M., Grandcolas, Philippe, Grande, Lance, Greenbaum, Eli, Gregorin, Renato, Grillitsch, Heinz, Grismer, Larry Lee, Grootaert, Patrick, Grosjean, Stéphane, Guarino, FABIO MARIA, Guayasamin, Juan M., Guénard, Benoit, Guevara, Lázaro, Guidoti, Marcu, Gupta, Devanshu, Gvoždík, Václav, Haddad, Célio F. 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S., De Lucena, Zilda Margarete S., Lucinda, Paulo, Lujan, Nathan K., Luporini, Pierangelo, Luz, David R., Lynch, John D., Machado, Leonardo Ferreira, Mahony, Stephen, Malabarba, Luiz R., Manuel Santos, Marivene, Marinho Filho, Jader, Marini, Miguel Â., Marques, Antonio Carlo, Marques, Mariana P., Mateus, Octávio, Matsui, Masafumi, Mazuch, Tomáš, Mccranie, Jame, Mckellar, Ryan C., Mcmahan, Caleb D., Mecke, Sven, Meißner, Karin, Mendoza Becerril, María A., Mendoza Palmero, Carlos A., Merker, Stefan, Mezzasalma, Marcello, Midgley, John Mark, Miller, Jeremy, Miller, Matthew J., Mincarone, Michael Maia, Minet, Joël, Miralles, Aurélien, Miranda, Thaís P., Missoup, Alain Didier, Modrý, David, Molinari, Jesú, Monadjem, Ara, Montreuil, Olivier, Moratelli, Ricardo, Moreira, Cristiano Rangel, Moreira, Felipe F. F., Mourer Chauviré, Cécile, Mulieri, Pablo Ricardo, Munroe, Thomas A., Naomi, Shun Ichiro, Nascimento, Fabio, Nässig, Wolfgang A., Neifar, Lassad, Netto Ferreira, Andre L., Niamir, Aidin, Nielsen, Stuart V., Nihei, Silvio S., Nistri, Annamaria, Oceguera Figueroa, Alejandro, Odierna, Gaetano, Ohler, Annemarie, Ojanguren Affilastro, Andres A., De Oliveira, Favízia Freita, De Oliveira, Marcio Luiz, De Oliveira, Otto Müller Patrão, Oliveira, Sarah Siqueira, Olson, Link E., Ong'Ondo, Geoffrey O., Orlov, Nikolai, Ornelas García, Claudia Patricia, Ortega, Hernan, Ortega Andrade, Mauricio, Ota, Hidetoshi, Pariselle, Antoine, Passos, Paulo, Pastana, Murilo N. L., Patterson, Bruce D., Patitucci, Luciano D., Patton, James L., Pavan, Ana C., Pavan, Silvia E., Pavia, Marco, Peloso, Pedro L. V., Pelzer, Alexander, Pereyra, Martín O., Perez Gonzalez, Abel, Pérez Luz, Blanca, Pérez, Cristian Hernan Fulvio, Peterhans, Julian Kerbi, Peterson, A. Townsend, Pétillon, Julien, Philips, Thomas Keith, Picariello, ORFEO LUCIO ANTONIO, Pie, Marcio R., Pikart, Tiago G., Pine, Ronald H., Pinheiro, Ulisse, Pinho, Luiz Carlo, Pinto, Ângelo P., Costa, Leonora Pire, Poggi, Roberto, Pombal, José P., Prabhu, Mrugank, Prendini, Elizabeth, Prendini, Lorenzo, Purushothaman, Jasmine, Pyron, Robert Alexander, Quintela Alonso, Pablo, Quinteros, Andres Sebastian, Quiroga Carmona, Marcial, Rabitsch, Wolfgang, Raffaëlli, Jean, Rage, Jean Claude, Rajaei, Hossein, Ramírez, Martín J., Raposo, Marcos A., Py Daniel, Lucia H. Rapp, Rasplus, Jean Yve, Ratcliffe, Brett C., Reddy, Sushma, Reis, Roberto E., Remsen, James V., Richards, Leigh R., Richling, Ira, Robillard, Tony, Rocha, Marcelo Salle, Rocha, Rosana Moreira, Rödder, Denni, Rödel, Mark Oliver, Rodrigues, Fernando P., Rodriguez, Estefania, Rogers, Duke S., Rojas Runjaic, Fernando J. M., Röll, Beate, Rosenberger, Alfred L., Rowley, Jodi, Roza, André Silva, Ruedi, Manuel, Salazar Bravo, Jorge, Salcedo, Norma J., Samyn, Yve, Santana, Sharlene E., Santoferrara, Luciana, Santos, Bernardo F., Santos, Charles Morphy D., Santos, Jean Carlo, Santos, Marcos Pérsio Danta, Sargis, Eric J., Schargel, Walter E., Schätti, Beat, Scherz, Mark D., Schlick Steiner, Birgit C., Schmidt, Ray C., Schmitt, Thoma, Schodde, Richard, Schoeman, Colin S., Schweiger, Silke, Schwertner, Cristiano F., Seamark, Ernest C. J., Semedo, Thiago B. F., Shin, Mann Kyoon, Siler, Cameron D., Silveira, Luís Fábio, Simison, W. Brian, Simões, Marcello, Sites, Jack W., Smith, Brian Tilston, Smith, Krister T., Song, Weibo, Soulier Perkins, Adeline, Sousa, Leandro M., Sparks, John S., Stampar, Sérgio N., Steiner, Florian M., Steyer, Jean Sébastien, Stiassny, Melanie L. J., Stoeck, Thorsten, Stopiglia, Renata, Streicher, Jeffrey W., Sturaro, Marcelo J., Stys, Pavel, Swierk, Lindsey, Taeger, Andrea, Takiya, Daniela M., Taphorn, Donald C., Tavares, Marco, Tavares, Valeria Da C., Taylor, Peter John, Tello, Jose G., Teta, Pablo, Tillack, Frank, Timm, Robert M., Tokaryk, Tim, Tominaga, Atsushi, Tonini, João Filipe Riva, Tornabene, Luke, Torres Carvajal, Omar, Townsend, Josiah, Trape, Jean Françoi, Rodrigues, Miguel Trefaut, Trusch, Robert, Tschopp, Emanuel, Uhl, Dieter, Upham, Nathan S., Vacher, Jean Pierre, Valdesalici, Stefano, Van Bocxlaer, Bert, Van Cakenberghe, Victor, Van De Kamp, Thoma, Van De Velde, Isabella, Van Den Spiegel, Didier, Vanhove, Maarten P. M., Vasudevan, Karthikeyan, Veerappan, Deepak, Velazco, Paúl M., Verdade, Vanessa K., Verheyen, Erik, Vieira, Leandro M., Victoriano, Pedro F., Vitt, Laurie J., Wagner, Philipp, Watkins Colwell, Gregory J., Weisse, Thoma, Werneck, Fernanda P., Wheeler, Ward C., Wilson, Don E., Valero, Katharina C. Wollenberg, Wood, Perry Lee, Woodman, Neal, Quetzalli, Hernández Díaz Yoalli, Yoshikawa, Natsuhiko, Zaher, Hussam, Ziegler, Thoma, Zima, Jan, Zink, Robert M., Zug, George, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Technische Universität Berlin (TU), Università degli Studi di Camerino (UNICAM), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade de Brasília, Institut de Systématique, Evolution, Biodiversité ( ISYEB ), Muséum National d'Histoire Naturelle ( MNHN ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ) -École pratique des hautes études ( EPHE ), Ecole Pratique des Hautes Etudes ( EPHE ), Centre National de la Recherche Scientifique ( CNRS ), Universidad Nacional de Tucumán, Villanova University [Philadelphie], University of Salzburg, Museu Paraense Emilio Goeldi, Universidade Federal do Pará, Pontificia Universidade Catolica do Rio Grande do Sul ( PUCRS ), Universidade Federal do Rio Grande do Sul ( UFRGS ), Royal Museum for Central Africa, Universidad Nacional Autónoma de México ( UNAM ), Technical University of Berlin, Universidade Federal de Pernambuco ( UFPE ), Universidade Federal do Espírito Santo ( UFES ), Institut de Recherche pour le Développement ( IRD [Nouvelle-Calédonie] ), Universidade Estadual Paulista Julio de Mesquita Filho ( UNESP ), Russian Academy of Sciences [Moscow] ( RAS ), Senckenberg Museum, Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ), Museu de Zoologia ( MZ ), Universidade de São Paulo ( USP ), North Dakota State University ( NDSU ), Departamento de Polícia Técnico Científica ( DPTC ), Louisiana State University ( LSU ), University of Tennessee, Ohio State University [Columbus] ( OSU ), Universidade Luterana do Brasil ( ULBRA ), University of Mississippi, Royal Belgian Institute of Natural Sciences ( RBINS ), University of New Mexico, Instituto Nacional de Pesquisas da Amazônia, Centre de Biologie pour la Gestion des Populations ( CBGP ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Federal Agency for the Safety of the Food Chain, Ceríaco, Luis M., Gutiérrez, Eliécer, Dubois, Alan Alqarni, Abdulaziz, Buckup, Paulo, Simón Abdala, Cristian, Algarni, abdulaziz, A. Adriano, Edson, Erna, Aescht, Villanova Univ, Museu Nacl Hist Nat & Ciencia, Universidade de Brasília (UnB), Smithsonian Inst, Sorbonne Univ, Univ Nacl Tucuman, King Saud Univ, Cornell Univ, Universidade Federal de São Paulo (UNIFESP), Upper Austrian Museum, Univ Salzburg, Zool Forsch Museum A Koenig, Russian Acad Sci, Pontificia Univ Catolica Rio Grande do Sul, Museo Reg Sci Nat, Univ Fed Rio Grande do Sul, Royal Museum Cent Africa, Univ Nacl Autonoma Mexico, Univ Innsbruck, Hasselt Univ, Universidade Federal de Pernambuco (UFPE), Univ Nacl Misiones, Natl Museum, Senckenberg Nat Hist Sammlungen, Universidade Estadual Paulista (Unesp), Stephen F Austin State Univ, Landesmuseum, Univ Camerino, Universidade Federal do ABC (UFABC), Universidade Estadual de Londrina (UEL), Senckenberg Res Inst, Museo Civ Storia Nat, Polskiej Akad Nauk, Harvard Univ, North West Univ, Museu Nacl, Nat Hist Museum, Senckenberg Nat Kundemuseum, North Carolina Museum Nat Sci, Univ Rennes 1, Amer Museum Nat Hist, Univ Huddersfield, North Dakota State Univ, Fac Sci Sfax, DPTC PC, Louisiana State Univ, Zool Survey India, Univ Tennessee, Ohio State Univ, Univ Luterana Brasil, Univ Firenze, Univ Ovidius Constanta, Univ Mississippi, Royal Belgian Inst Nat Sci, Univ New Mexico, Inst Nacl de Pesquisas da Amazonia, Univ Stellenbosch, Universidade Federal de Minas Gerais (UFMG), CIBIO Ctr Invest Biodiversidade & Recursos Genet, Museum Hist Nat, Universidade Federal de Mato Grosso do Sul (UFMS), Univ Austral Chile, Univ Malaysia, Indian Inst Sci, Scottish Assoc Marine Sci, Sam Houston State Univ, Museo Nacl Ciencias Nat, Drexel Univ, Univ Richmond, Ctr Pesquisas Cacau, Univ Torino, Soc Hist Nat Alcide dOrbigny, Wolfden Sci Consulting, Universidade Federal do Rio de Janeiro (UFRJ), Inst Humboldt, Escuela Politec Nacl, Calif Acad Sci, Museum Dept Hist Nat Var, Nat Environm & Wildlife Soc, Univ Kansas, Kyoto Univ, Consejo Nacl Invest Cient & Tecn, Univ Fed Paraiba, Univ Fed Parana, Nat Hist Museum Narodini Museum, Nat Hist Museum Zimbabwe, Ateneo Manila Univ, Pontificia Univ Javeriana, RACINE, Univ Paris 06, Ocean Univ China, Museo Nacl Hist Nat Paraguay, Univ Carabobo, Natl Ctr Biol Sci, Univ Nebraska, CENAK Ctr Nat Kunde, Ohio Hist Connect, Univ Fed Amazonas, Univ Malaga, Chinese Acad Sci, Benemerita Univ Autonoma Puebla, Natl Polytech Inst, Field Museum Nat Hist, Univ Texas El Paso, Universidade Federal de Lavras (UFLA), La Sierra Univ, Univ San Francisco Quito, Univ Hong Kong, CUNY, CAS, Zool Staatssammlung Munchen, Univ Michigan, Helmholtz Ctr Environm Res, Santa Rosa Jr Coll, George Washington Univ, Inst Teknol Bandung, Earlham Coll, Senckenberg Forschungsinst & Nat Museum, Univ Koblenz Landau, Ditsong Natl Museum Nat Hist, Bombay Nat Hist Soc, Philipps Univ Marburg, Hess Landesmuseum, Smithsonian Conservat Biol Inst, Univ Pannonia, Staatliches Museum Nat Kunde, UFT, Museum Nat Kunde, Nat Hist Riksmuseet, Hikarigaoka, Inst Agr & Environm Sci, Univ Los Andes, Bat Jungle, Texas A&M Univ, Yale Univ, Museo Nacl Hist Nat, Roger Tory Peterson Inst Nat Hist, Univ Fed Reconcavo Bahia, South China Normal Univ, Museu Ciencias Tecnol PUCRS, Univ Fed Tocantins, Univ Toronto, Univ Nacl Colombia, Natl Museum Philippines, NOVA Univ Lisbon, Royal Saskatchewan Museum, Deutsch Zentrum Marine Biodiversitatsforsch, Ctr Invest Biol Noroeste, Naturalis Biodivers Ctr, Univ Douala, Vet & Farmaceut Univ Brno, Univ Swaziland, Fundacao Oswaldo Cruz, Univ Claude Bernard, Museum Vertebrate Zool, Nat Hist Museum & Inst, Senckenberg Biodiversitat & Klima Forschunsgzentr, Marquette Univ, Universidade Federal da Bahia (UFBA), Fed Univ ABC, Universidade Federal de Goiás (UFG), Univ Alaska Museum, Egerton Univ, Museo Hist Nat, IKIAM Univ Reg Amazon, Univ Hyogo, Inst Rech Dev, Niedersachs Landesbetrieb Wasserwirtschaft Kusten, Univ Complutense Madrid, Roosevelt Univ, Western Kentucky Univ, Univ Naples Federico II, Univ Fed Acre, Universidade Federal de Santa Catarina (UFSC), Inst Bio & Geociencias Noroeste Argentino, Inst Venezolano Invest Cient, Umweltbundesamt, Penclen, CNRS MNHN UPMC, Staatl Museum Nat Kunde, Ctr Biol Gest Populat INRA, Loyola Univ Chicago, Pontificia Univ Catolica Rio do Sul, Durban Museum Nat Sci, Univ Estado Amazonas, Brigham Young Univ, Museo Hist Nat La Salle, Univ Vet Med Hannover, Australian Museum, Texas Tech Univ, Francis Marion Univ, Univ Washington, Univ Connecticut, Universidade Federal de Uberlândia (UFU), Fed Univ Para, Yale Peabody Museum, Univ Texas Arlington, Senckenberg Deutsch Entomol Inst, CSIRO, Univ Venda, Univ Ulsan, Senckenberg Forsch Inst & Nat Museum, Univ Fed Para, UPMC, Tech Univ Kaiserslautern, Charles Univ Prague, Univ Nacl Expt los Llanos Occident Ezequiel Zamor, Long Isl Univ, Univ Ryukyus, Pontificia Univ Catolica Ecuador, Indiana Univ Penn, IRD, State Museum Nat Hist Karlsruhe, Univ Toulouse III Paul Sabatier, Univ Ghent, Univ Antwerp, Karlsruhe Inst Technol, Ctr Cellular & Mol Biol, Yale Peabody Museum Nat Hist, Bethune Cookman Univ, Natl Museum Nat & Sci, and Zool Garten Koln
Subjects: 0106 biological sciences, [SDV]Life Sciences [q-bio], Rebuttal, 010607 zoology, Biology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Q1, 010603 evolutionary biology, 01 natural sciences, Biological Science Disciplines, FOTOGRAFIA, Photography, Animals, Animal species, Biological sciences, QH426, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, Taxonomy, QL, [ SDV ] Life Sciences [q-bio], Ecology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biodiversity, Classification, Ecology, Evolution, Behavior and Systematic, Taxonomy (biology), Animal Science and Zoology, Classics
Abstract: Made available in DSpace on 2021-06-25T12:17:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-23 Villanova Univ, Dept Biol, Villanova, PA 19085 USA Museu Nacl Hist Nat & Ciencia, Lisbon, Portugal Univ Brasilia, Dept Zool, Inst Ciencias Biol, BR-70910900 Brasilia, DF, Brazil Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20560 USA Sorbonne Univ, Museum Natl Hist Nat, ISYEB, Paris, France Univ Nacl Tucuman, San Miguel De Tucuman, Argentina King Saud Univ, Riyadh, Saudi Arabia Cornell Univ, Ithaca, NY USA Univ Fed Sao Paulo, Diadema, Brazil Upper Austrian Museum, Ctr Biol, Linz, Austria Villanova Univ, Villanova, PA 19085 USA Univ Salzburg, Salzburg, Austria Plazi, Bern, Switzerland Univ Brasilia, Brasilia, DF, Brazil Univ Sao Paulo, Ribeirao Preto, Brazil Zool Forsch Museum A Koenig, Bonn, Germany Museu Paraense Emilio Goeldi, Belem, Para, Brazil Russian Acad Sci, St Petersburg, Russia Pontificia Univ Catolica Rio Grande do Sul, Porto Alegre, RS, Brazil Museo Reg Sci Nat, Turin, Italy Univ Fed Rio Grande do Sul, Porto Alegre, RS, Brazil Royal Museum Cent Africa, Tervuren, Belgium Univ Nacl Autonoma Mexico, Mexico City, DF, Mexico Univ Innsbruck, Inst Ecol, Innsbruck, Austria Hasselt Univ, Hasselt, Belgium Univ Fed Pernambuco, Recife, PE, Brazil Univ Fed Espirito Santo, Vitoria, ES, Brazil Univ Nacl Misiones, Felix De Azara, Argentina Albany Museum, Grahamstown, South Africa Natl Museum, Bloemfontein, South Africa Senckenberg Nat Hist Sammlungen, Dresden, Germany Univ Estadual Paulista, Botucatu, SP, Brazil Stephen F Austin State Univ, Nacogdoches, TX 75962 USA Landesmuseum, Leonding, Austria Univ Camerino, Camerino, Italy Univ Fed ABC, Sao Bernardo, Brazil Univ Estadual Londrina, Museu Zool, Londrina, Parana, Brazil Senckenberg Res Inst, Frankfurt, Germany Museo Civ Storia Nat, Carmagnola, Italy Polskiej Akad Nauk, Muzeum & Inst Zool, Warsaw, Poland Museo Civ Storia Nat, Genoa, Italy Russian Acad Sci, Inst Zool, St Petersburg, Russia Univ Estadual Paulista, Sao Vicente, Brazil Sorbonne Univ, ISYEB, Museum Natl Hist Nat, Paris, France Port Elizabeth Museum, Port Elizabeth, South Africa Sam Noble Museum, Norman, OK USA Harvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA North West Univ, Potchefstroom, South Africa Museu Nacl, Rio De Janeiro, Brazil Nat Hist Museum, Basel, Switzerland Senckenberg Nat Kundemuseum, Gorlitz, Germany North Carolina Museum Nat Sci, Raleigh, NC USA Univ Rennes 1, Rennes, France Univ Sao Paulo, Museu Zool, Sao Paulo, SP, Brazil Amer Museum Nat Hist, New York, NY 10024 USA Univ Huddersfield, Huddersfield, W Yorkshire, England North Dakota State Univ, Fargo, ND USA Univ Sao Paulo, Sao Paulo, SP, Brazil Fac Sci Sfax, Sfax, Tunisia DPTC PC, Manaus, Amazonas, Brazil Louisiana State Univ, Museum Nat Sci, Baton Rouge, LA 70803 USA Zool Survey India, Kolkata, India Univ Tennessee, Chattanooga, TN USA Ohio State Univ, Columbus, OH 43210 USA Univ Luterana Brasil, Museu Ciencias Nat, Canoas, Brazil Univ Firenze, Florence, Italy Univ Ovidius Constanta, Constanta, Romania Univ Mississippi, Oxford, MS USA Royal Belgian Inst Nat Sci, Brussels, Belgium Univ New Mexico, Albuquerque, NM 87131 USA Inst Nacl de Pesquisas da Amazonia, Manaus, Amazonas, Brazil Univ Stellenbosch, Matieland, South Africa Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil CIBIO Ctr Invest Biodiversidade & Recursos Genet, Vairao, Portugal Museum Hist Nat, Geneva, Switzerland Univ Fed Mato Grosso, Cuiaba, Brazil Univ Austral Chile, Valdivia, Chile Univ Malaysia, Sarawak, Malaysia Indian Inst Sci, Bangalore, Karnataka, India Scottish Assoc Marine Sci, Oban, Argyll, Scotland Sam Houston State Univ, Huntsville, TX 77340 USA Museo Nacl Ciencias Nat, Madrid, Spain Sorbonne Univ, CR2P, Museum Natl Hist Nat, Paris, France Drexel Univ, Acad Nat Sci, Philadelphia, PA 19104 USA Univ Richmond, Richmond, VA 23173 USA Ctr Pesquisas Cacau, Itabuna, Brazil Univ Torino, Turin, Italy Soc Hist Nat Alcide dOrbigny, Aubiere, France Wolfden Sci Consulting, Murcia, Spain Univ Fed Rio de Janeiro, Macae, Brazil Univ Tennessee, Knoxville, TN USA Inst Humboldt, Villa De Leyva, Colombia Escuela Politec Nacl, Quito, Ecuador Calif Acad Sci, San Francisco, CA 94118 USA Museum Dept Hist Nat Var, Toulon, France Nat Environm & Wildlife Soc, Angul, India Univ Kansas, Lawrence, KS 66045 USA Kyoto Univ, Kyoto, Japan Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivadavia, Buenos Aires, DF, Argentina Univ Fed Paraiba, Joao Pessoa, Paraiba, Brazil Univ Fed Parana, Curitiba, Parana, Brazil Univ Fed Rio de Janeiro, Rio De Janeiro, Brazil Nat Hist Museum Narodini Museum, Prague, Czech Republic Nat Hist Museum Zimbabwe, Bulawayo, Zimbabwe Ateneo Manila Univ, Quezon City, Philippines Pontificia Univ Javeriana, Bogota, Colombia RACINE, St Maugan, France Univ Paris 06, Sorbonne Univ Paris, ISYEB, Paris, France Ocean Univ China, Qingdao, Peoples R China Museo Nacl Hist Nat Paraguay, San Lorenzo, Paraguay Univ Carabobo, Valencia, Venezuela Natl Ctr Biol Sci, Bengaluru, India Univ Nebraska, Lincoln, NE USA CENAK Ctr Nat Kunde, Hamburg, Germany Ohio Hist Connect, Columbus, OH USA Univ Fed Amazonas, Manaus, Amazonas, Brazil Univ Malaga, Malaga, Spain Chinese Acad Sci, Qingdao, Shandong, Peoples R China Benemerita Univ Autonoma Puebla, Puebla, Mexico Natl Polytech Inst, Ctr Res & Adv Studies, Irapuato, Mexico Field Museum Nat Hist, Chicago, IL 60605 USA Univ Texas El Paso, El Paso, TX 79968 USA Univ Fed Lavras, Lavras, Brazil Nat Hist Museum, Vienna, Austria La Sierra Univ, Riverside, CA USA Univ San Francisco Quito, Quito, Ecuador Univ Hong Kong, Hong Kong, Peoples R China CUNY, New York, NY 10021 USA CAS, Inst Vertebrate Biol, Brno, Czech Republic Sorbonne Univ, MECADEV, Museum Natl Hist Nat, Paris, France Univ Estadual Paulista, Rio Claro, Brazil Zool Staatssammlung Munchen, Munich, Germany Univ Michigan, Dearborn, MI 48128 USA Smithsonian Inst, Natl Museum Nat Hist, Washington, DC USA Helmholtz Ctr Environm Res, Leipzig, Germany Santa Rosa Jr Coll, Santa Rosa, CA USA George Washington Univ, Washington, DC 20037 USA Inst Teknol Bandung, Bandung, Indonesia Earlham Coll, Richmond, IN USA Senckenberg Forschungsinst & Nat Museum, Frankfurt, Germany Univ Estadual Londrina, Londrina, Parana, Brazil Univ Koblenz Landau, Koblenz, Germany Nat Hist Museum, London, England Ditsong Natl Museum Nat Hist, Pretoria, South Africa Bombay Nat Hist Soc, Bombay, Maharashtra, India Philipps Univ Marburg, Marburg, Germany Hess Landesmuseum, Darmstadt, Germany Smithsonian Conservat Biol Inst, Washington, DC USA Univ Pannonia, Keszthely, Hungary Staatliches Museum Nat Kunde, Stuttgart, Germany UFT, Tocantins, Portugal Museum Nat Kunde, Berlin, Germany Nat Hist Riksmuseet, Stockholm, Sweden Hikarigaoka, Munakata, Japan Inst Agr & Environm Sci, Tartu, Estonia Univ Los Andes, Merida, Venezuela Bat Jungle, Monteverde, Costa Rica Texas A&M Univ, College Stn, TX USA Yale Univ, New Haven, CT USA Univ Estadual Paulista, Sao Jose Do Rio Preto, Brazil Museo Nacl Hist Nat, Montevideo, Uruguay Consejo Nacl Invest Cient & Tecn, Fdn Miguel Lillo, San Miguel De Tucuman, Argentina Roger Tory Peterson Inst Nat Hist, Jamestown, VA USA Univ Fed Reconcavo Bahia, Cruz Das Almas, Brazil South China Normal Univ, Guangzhou 510631, Guangdong, Peoples R China Museu Ciencias Tecnol PUCRS, Porto Alegre, RS, Brazil Univ Fed Tocantins, Porto Nacl, Brazil Univ Toronto, Scarborough, ON, Canada Univ Nacl Colombia, Inst Ciencias Nat, Bogota, Colombia Natl Museum Philippines, Manila, Philippines NOVA Univ Lisbon, Caparica, Portugal Royal Saskatchewan Museum, Regina, SK, Canada Deutsch Zentrum Marine Biodiversitatsforsch, Hamburg, Germany Ctr Invest Biol Noroeste, La Paz, Mexico Naturalis Biodivers Ctr, Leiden, Netherlands Univ Douala, Douala, Cameroon Vet & Farmaceut Univ Brno, Brno, Czech Republic Univ Swaziland, Kwaluseni, Eswatini Fundacao Oswaldo Cruz, Rio De Janeiro, Brazil Univ Claude Bernard, Lyon, France Museum Vertebrate Zool, Berkeley, CA USA Smithsonian Inst, Washington, DC 20560 USA Nat Hist Museum & Inst, Chiba, Japan Senckenberg Biodiversitat & Klima Forschunsgzentr, Frankfurt, Germany Marquette Univ, Milwaukee, WI 53233 USA Univ Firenze, Museo Storia Nat, Florence, Italy Univ Fed Bahia, Salvador, BA, Brazil Fed Univ ABC, Sao Bernardo, Brazil Univ Fed Goias, Goiania, Go, Brazil Univ Alaska Museum, Fairbanks, AK USA Egerton Univ, Egerton, Kenya Museo Hist Nat, Lima, Peru IKIAM Univ Reg Amazon, Tena, Ecuador Univ Hyogo, Sanda, Japan Inst Rech Dev, Paris, France Univ Sao Paulo, Piracicaba, Brazil Niedersachs Landesbetrieb Wasserwirtschaft Kusten, Hannover, Germany Univ Complutense Madrid, Madrid, Spain Consejo Nacl Invest Cient & Tecn, Ctr Nacl Patagon, Puerto Madryn, Argentina Roosevelt Univ, Coll Profess Studies, Chicago, IL 60605 USA Western Kentucky Univ, Bowling Green, KY 42101 USA Univ Naples Federico II, Naples, Italy Univ Fed Acre, Rio Branco, Acre, Brazil Univ Fed Santa Catarina, Florianopolis, SC, Brazil Inst Bio & Geociencias Noroeste Argentino, Salta, Argentina Inst Venezolano Invest Cient, Caracas, Venezuela Umweltbundesamt, Vienna, Austria Penclen, Plumelec, France CNRS MNHN UPMC, Ctr Rech Paleobiodivers & Paleoenvironm, Paris, France Staatl Museum Nat Kunde, Stuttgart, Germany Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivada, Buenos Aires, DF, Argentina Ctr Biol Gest Populat INRA, Montferrier Sur Lez, France Loyola Univ Chicago, Chicago, IL USA Pontificia Univ Catolica Rio do Sul, Porto Alegre, RS, Brazil Durban Museum Nat Sci, Durban, South Africa Univ Estado Amazonas, Manaus, Amazonas, Brazil Brigham Young Univ, Provo, UT 84602 USA Museo Hist Nat La Salle, Caracas, Venezuela Univ Vet Med Hannover, Hannover, Germany Australian Museum, Sydney, NSW, Australia Texas Tech Univ, Lubbock, TX 79409 USA Francis Marion Univ, Florence, SC USA Univ Washington, Seattle, WA 98195 USA Univ Connecticut, Groton, CT USA Fed Univ ABC, Santo Andre, SP, Brazil Univ Fed Uberlandia, Uberlandia, MG, Brazil Fed Univ Para, Belem, Para, Brazil Yale Peabody Museum, New Haven, CT USA Univ Texas Arlington, Arlington, TX 76019 USA Senckenberg Deutsch Entomol Inst, Muncheberg, Germany CSIRO, Natl Res Collect, Canberra, ACT, Australia Univ Venda, Thohoyandou, South Africa Univ Ulsan, Ulsan, South Korea Univ Estadual Sao Paulo, Botucatu, SP, Brazil Senckenberg Forsch Inst & Nat Museum, Frankfurt, Germany Ocean Univ China, Inst Marine Biodivers & Evolut, Qingdao, Shandong, Peoples R China Univ Fed Para, Altamira, Brazil Univ Estadual Paulista, Assis, Brazil UPMC, Ctr Rech Paleobiodiversite & Paleoenvironm, CNRS, MNHN, Paris, France Tech Univ Kaiserslautern, Kaiserslautern, Germany Charles Univ Prague, Dept Zool, Prague, Czech Republic Univ Nacl Expt los Llanos Occident Ezequiel Zamor, Guanare, Venezuela Long Isl Univ, Brooklyn, NY USA Univ Ryukyus, Okinawa, Japan Pontificia Univ Catolica Ecuador, Museo Zool, Escuela Ciencias Biol, Quito, Ecuador Indiana Univ Penn, Indiana, PA USA IRD, Dakar, Senegal State Museum Nat Hist Karlsruhe, Karlsruhe, Germany Univ Toulouse III Paul Sabatier, Toulouse, France Univ Ghent, Ghent, Belgium Univ Antwerp, Antwerp, Belgium Karlsruhe Inst Technol, Karlsruhe, Germany Ctr Cellular & Mol Biol, Hyderabad, India Univ Fed ABC, Santo Andre, Brazil Yale Peabody Museum Nat Hist, New Haven, CT USA Univ Innsbruck, Mondsee, Austria Bethune Cookman Univ, Daytona Beach, FL USA Natl Museum Nat & Sci, Tokyo, Japan Zool Garten Koln, Cologne, Germany Univ Estadual Paulista, Botucatu, SP, Brazil Univ Estadual Paulista, Sao Vicente, Brazil Univ Estadual Paulista, Rio Claro, Brazil Univ Estadual Paulista, Sao Jose Do Rio Preto, Brazil Univ Estadual Paulista, Assis, Brazil
Published: 2016
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42. Species of thrips (Insecta, Thysanoptera) in two strawberry production systems in Rio Grande do Sul State, Brazil

Author: Luiza Rodrigues Redaelli, Marcos Botton, Aline Nondillo, Carlos Eduardo da Cunha Pinent, Silvia Marisa Jesien Pinent, Silvia M. J. Pinent, UFRGS, Aline Nondillo, UFRGS, MARCOS BOTTON, CNPUV, Luiza R. Redaelli, UFRGS, and Carlos E. da C. Pinent, UCS.
Subjects: Quantificação, thysanopterans, Gemina, Sistema de Produção, Trips, Genus, levantamento de espécies, Botany, lcsh:Zoology, lcsh:QL1-991, Food resource, Entomologia agricola, tisanópteros, biology, Thrips, General Engineering, Thripidae, biology.organism_classification, Morango, Fruticultura, Heterothrips, Praga de planta, species survey, Caliothrips fasciatus, Heterothripidae, Fragaria x ananassa
Abstract: Species of thrips (Insecta, Thysanoptera) in two strawberry production systems in Rio Grande do Sul, Brazil. Thrips are tiny insects responsible for the reduction of strawberry fruit quality. The work aimed to record and quantify the thysanopterofauna present in two strawberry production systems, low tunnel and semi-hydroponic. Leaves, flowers and fruits were collected weekly, from July 2005 to December 2006 in Caxias do Sul and Bom Princípio municipalities, RS. A total of 664 individuals were collected, representing two families, four genus and 10 species: Frankliniella occidentalis (Pergande, 1895), F. schultzei (Trybom, 1910), F. rodeos Moulton, 1933, F. simplex (Priesner, 1924), F. williamsi (Hood, 1915), F. gemina (Bagnall, 1919), Frankliniella sp., Thrips tabaci (Lindeman, 1888), Thrips tabaci (Lindeman, 1888), Caliothrips fasciatus (Pergande 1895) from Thripidae and Heterothrips sp. from Heterothripidae. Frankliniella occidentalis represented 89.7% of the samples with 95.8% of the species collected in flowers, 3.9% in fruits and 0.8% in leaves. The results show that flowers are the most important food resource for these insects on strawberry plants. Frankliniella rodeos, F. simplex, F. williamsi, C. fasciatus, and Heterothrips sp. are new records on strawberry for Brazil.Espécies de tripes (Insecta, Thysanoptera) associadas à cultura do morangueiro em dois sistemas de produção no Rio Grande do Sul, Brasil. Tripes são insetos diminutos responsáveis pela redução da qualidade dos frutos do morangueiro. O trabalho objetivou registrar e quantificar a tisanopterofauna presente em dois sistemas de produção de morangueiro, túnel baixo e semihidropônico. Folhas, flores e frutos foram coletados semanalmente, de julho de 2005 a dezembro de 2006 em Caxias do Sul e Bom Princípio, RS. Foram coletados 664 indivíduos pertencentes a duas famílias, quatro gêneros e 10 espécies: Frankliniella occidentalis (Pergande, 1895), F. schultzei (Trybom, 1910), F. rodeos (Moulton, 1933), F. simplex (Priesner, 1924), F. williamsi (Hood, 1915), F. gemina (Bagnall, 1919), Frankliniella sp., Thrips tabaci (Lindeman, 1888) e Caliothrips fasciatus (Pergande, 1895) pertencentes a Thripidae, e Heterothrips sp. pertencente a Heterothripidae. Com 593 ocorrências, F. occidentalis representou 89,3% dos insetos coletados, com 87,9% dos indivíduos nas flores, 8,4% nas folhas e 3,7% nos frutos. Os resultados sugerem que as flores do morangueiro representam o recurso preferido pelos tripes. Frankliniella rodeos, F. simplex, F. williamsi, C. fasciatus e Heterothrips sp. são novos registros na cultura para o Brasil.
Published: 2011

43. Injúrias causadas por mosca-das-frutas sul-americana em cultivares de videira

Author: Marcelo Zart, Odair Aparecido Fernandes, Marcos Botton, MARCELO ZART, UFRGS, MARCOS BOTTON, CNPUV, Odair Aparecido Fernandes, UNESP., Universidade Federal do Rio Grande do Sul (UFRGS), Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), and Universidade Estadual Paulista (Unesp)
Subjects: Vitis labrusca, Materials Science (miscellaneous), Berry, Biology, desenvolvimento de larvas, berries dropping, Tephritidae, Botany, mosca-das-frutas sul-americana, Cultivar, lcsh:Agriculture (General), Phenology, Viticultura, queda de bagas, Cabernet Sauvignon, Anastrepha fraterculus, Moscato Embrapa, biology.organism_classification, lcsh:S1-972, Isabel, Pupa, Mosca-das-frutas, Horticulture, Uva, Praga de planta, South american, south american fruit fly, Vitis vinifera, PEST analysis, Bento Gonçalves, General Agricultural and Biological Sciences, Dano, development of larvae
Abstract: Submitted by Guilherme Lemeszenski (guilherme@nead.unesp.br) on 2013-08-22T18:53:53Z No. of bitstreams: 1 S0006-87052011000100011.pdf: 2063400 bytes, checksum: f44abb903f0c039da36dcc4f54c32568 (MD5) Made available in DSpace on 2013-08-22T18:53:53Z (GMT). No. of bitstreams: 1 S0006-87052011000100011.pdf: 2063400 bytes, checksum: f44abb903f0c039da36dcc4f54c32568 (MD5) Previous issue date: 2011-01-01 Made available in DSpace on 2013-09-30T17:54:01Z (GMT). No. of bitstreams: 2 S0006-87052011000100011.pdf: 2063400 bytes, checksum: f44abb903f0c039da36dcc4f54c32568 (MD5) S0006-87052011000100011.pdf.txt: 37727 bytes, checksum: 58d38c93048dd68df7851608dea19e36 (MD5) Previous issue date: 2011-01-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T13:15:01Z No. of bitstreams: 2 S0006-87052011000100011.pdf: 2063400 bytes, checksum: f44abb903f0c039da36dcc4f54c32568 (MD5) S0006-87052011000100011.pdf.txt: 37727 bytes, checksum: 58d38c93048dd68df7851608dea19e36 (MD5) Made available in DSpace on 2014-05-20T13:15:01Z (GMT). No. of bitstreams: 2 S0006-87052011000100011.pdf: 2063400 bytes, checksum: f44abb903f0c039da36dcc4f54c32568 (MD5) S0006-87052011000100011.pdf.txt: 37727 bytes, checksum: 58d38c93048dd68df7851608dea19e36 (MD5) Previous issue date: 2011-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A mosca-das-frutas sul-americana, Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae), é uma das principais pragas da fruticultura no Brasil. O objetivo deste estudo foi identificar a suscetibilidade quanto à queda e deformação de bagas de diferentes cultivares/espécies de uva pela injúria causada pela oviposição de A. fraterculus. O trabalho foi realizado em vinhedos das cultivares 'Cabernet Sauvignon' (Vitis vinifera), 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)' (75% de V. vinifera) e 'Isabel' (Vitis labrusca), localizados em Bento Gonçalves (RS), na safra 2006/2007. Dois casais de A. fraterculus foram confinados por cacho, em gaiolas de tecido voile, durante os estádios fenológicos de grão ervilha, início da compactação, início da maturação e maturação plena dos cachos. Queda significativa de bagas devido ao ataque de A. fraterculus foi registrada na cultivar 'Cabernet Sauvignon' quando a infestação foi realizada nos estádios fenológicos de grão ervilha e início da compactação de cacho; estádios de grão ervilha em 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)'; e nos estádios de grão ervilha, início da compactação e início da maturação do cacho na cultivar 'Isabel'. Foi registrada deformação de bagas quando as infestações foram realizadas nos estádios de grão ervilha e início da compactação de cacho em 'Cabernet Sauvignon', 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)' e 'Isabel'. O desenvolvimento larval até a fase de pupa foi observado somente na cultivar 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)'. The South American Fruit Fly, Anastrepha fraterculus (Wiedemann) (Diptera: Tephritidae), is a major pest of fruit production in Brazil. This study was conducted to evaluate the stages of berry susceptibility and injuries caused by the fruit flies in different grape cultivars. The work was conducted in the field with 'Cabernet Sauvignon', 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)' and 'Isabel' cultivars, located in Bento Gonçalves, RS, in 2006 and 2007. Two couples of A. fraterculus were confined in grape bunchs in different phenological stages: berries pea-size, berries still hard and green, berries begin to colour and enlarge, and berries harvest-ripe. Significative berry dropping was recorded in 'Cabernet Sauvignon' cultivar when injured by A. fraterculus in the stages of berries pea-size and berries still hard and green; stage of berries pea-size in 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)' and stages of berries pea-size, berries still hard and green, berries begin to colour and enlarge in 'Isabel' cultivar. Deformation in berries was recorded when infestations were done in phases berries pea-size and berries still hard and green of 'Cabernet Sauvignon', 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)' and 'Isabel' cultivars. There was development of larvae to the pupa stage in 'Moscato Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA)' cultivar. UFRGS Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA) Uva e Vinho CNPUV UNESP FCAV Departamento de Fitossanidade UNESP FCAV Departamento de Fitossanidade
Published: 2011

44. Identification and expression analysis of genes associated with the early berry development in the seedless grapevine (Vitis vinifera L.) cultivar Sultanine

Author: Danielle Costenaro-da-Silva, Luís Fernando Revers, João Antonio Pêgas Henriques, Giancarlo Pasquali, Gisele Passaia, Rogério Margis, DANIELLE COSTENARO-DA-SILVA, UFRGS, GISELE PASSAIA, CNPUV (bolsista), JOÃO A. P. HENRIQUES, UFRGS, ROGÉRIO MARGIS, UFRGS, GIANCARLO PASQUALI, UFRGS, and LUIS FERNANDO REVERS, CNPUV.
Subjects: Candidate gene, RDA, In silico, Plant Science, Berry, Expressão gênica, Biology, Uva de mesa, PCR em tempo real, Reference genes, Botany, Genetics, Gene, Biologia molecular, Expressed sequence tag, Identificação genética, Viticultura, fungi, General Medicine, Genética, Phenotype, Desenvolvimento da baga, Uva sem semente, Uva, Representational difference analysis, Agronomy and Crop Science
Abstract: Sultanine grapevine (Vitis vinifera L.) is one of the most important commercial seedless table-grape varieties and the main source of seedlessness for breeding programs around the world. Despite its commercial relevance, little is known about the genetic control of seedlessness in grapes, remaining unknown the molecular identity of genes responsible for such phenotype. Actually, studies concerning berry development in seedless grapes are scarce at the molecular level. We therefore developed a representational difference analysis (RDA) modified method named Bulk Representational Analysis of Transcripts (BRAT) in the attempt to identify genes specifically associated with each of the main developmental stages of Sultanine grapevine berries. A total of 2400 transcript-derived fragments (TDFs) were identified and cloned by RDA according to three specific developmental berry stages. After sequencing and in silico analysis, 1554 (64.75%) TDFs were validated according to our sequence quality cut-off. The assembly of these expressed sequence tags (ESTs) yielded 504 singletons and 77 clusters, with an overall EST redundancy of approximately 67%. Amongst all stage-specific cDNAs, nine candidate genes were selected and, along with two reference genes, submitted to a deeper analysis of their temporal expression profiles by reverse transcription-quantitative PCR. Seven out of nine genes proved to be in agreement with the stage-specific expression that allowed their isolation by RDA. Made available in DSpace on 2019-04-27T00:41:45Z (GMT). No. of bitstreams: 1 PlantScienceRevers2010.pdf: 520013 bytes, checksum: fe16ec2ebc986ea8d6298e5435908e08 (MD5) Previous issue date: 2010
Published: 2010
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45. MYB-FL controls gain and loss of floral UV absorbance, a key trait affecting pollinator preference and reproductive isolation

Author: Loreta B. Freitas, Michel Moser, Therese Mandel, Hester Sheehan, Michiel Vandenbussche, Korinna Esfeld, Sabine Metzger, Alexandre Dell’Olivo, Cris Kuhlemeier, Ulrich Klahre, Institute of Plant Sciences, University of Bern, University of Cologne, Reproduction et développement des plantes (RDP), Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de la Recherche Agronomique (INRA)-École normale supérieure - Lyon (ENS Lyon), Universidade Federal do Rio Grande do Sul (UFRGS), ATIP-AVENIR (CNRS), ANR-BLANC grant, Science without Borders, CNPq, National Centre of Competence in Research, Swiss National Science Foundation, University of Bern, École normale supérieure de Lyon (ENS de Lyon)-Institut National de la Recherche Agronomique (INRA)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Centre National de la Recherche Scientifique (CNRS), and Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS)
Subjects: 0301 basic medicine, animal structures, Pollination, DNA, Plant, Ultraviolet Rays, [SDV]Life Sciences [q-bio], Molecular Sequence Data, Flowers, 580 Plants (Botany), Petunia, Frameshift mutation, 03 medical and health sciences, Pollinator, biology.animal, Manduca, Botany, Genetics, Animals, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, MYB, Amino Acid Sequence, biology, Base Sequence, Reproduction, digestive, oral, and skin physiology, fungi, Reproductive isolation, biology.organism_classification, 030104 developmental biology, Evolutionary biology, Mutation (genetic algorithm), Hummingbird
Abstract: International audience; Adaptations to new pollinators involve multiple floral traits, each requiring coordinated changes in multiple genes. Despite this genetic complexity, shifts in pollination syndromes have happened frequently during angiosperm evolution. Here we study the genetic basis of floral UV absorbance, a key trait for attracting nocturnal pollinators. In Petunia, mutations in a single gene, MYB-FL, explain two transitions in UV absorbance. A gain of UV absorbance in the transition from bee to moth pollination was determined by a cis-regulatory mutation, whereas a frameshift mutation caused subsequent loss of UV absorbance during the transition from moth to hummingbird pollination. The functional differences in MYB-FL provide insight into the process of speciation and clarify phylogenetic relationships between nascent species.
Published: 2016
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46. Structural genomics and transcriptional characterization of the Dormancy-Associated MADS-box genes during bud dormancy progression in apple

Author: Henrique Pessoa dos Santos, Flávio Bello Fialho, Vítor da Silveira Falavigna, Diogo Denardi Porto, Paulo Ricardo Dias de Oliveira, Rafael Augusto Arenhart, Luís Fernando Revers, Rafael Anzanello, Pâmela Perini, Vanessa Buffon, DIOGO DENARDI PORTO, CPATSA, VÍTOR DA SILVEIRA FALAVIGNA, UFRGS, RAFAEL AUGUSTO ARENHART, UFRGS, PÂMELA PERINI, Instituto Federal de Educação, Ciência e Tecnologia do Rio Grande do Sul, VANESSA BUFFON, CNPUV, RAFAEL ANZANELLO, Fundação Estadual de Pesquisa Agropecuária, Veranópolis, RS, HENRIQUE PESSOA DOS SANTOS, CNPUV, FLAVIO BELLO FIALHO, CNPUV, PAULO RICARDO DIAS DE OLIVEIRA, CNPUV, and LUIS FERNANDO REVERS, CNPUV.
Subjects: 0106 biological sciences, 0301 basic medicine, Candidate gene, Malus, Bud dormancy, Growth regulator, Locus (genetics), Horticulture, 01 natural sciences, Quebra de dormência, 03 medical and health sciences, Collinearity, Genetics, Malus Domestica, Molecular Biology, Gene, MADS-box, Messenger RNA, biology, DAM genes, Regulador de crescimento, Intron, Apple, Forestry, biology.organism_classification, Maçã, 030104 developmental biology, Genes DAM, Dormancy, 010606 plant biology & botany
Abstract: The molecular control of bud dormancy establishment and release is still not well understood, although some genes have already been demonstrated to play important roles in this process. The dormancy-associated MADS-box (DAM) genes were first identified in the peach EVERGROWING locus and are considered the main regulators of bud dormancy control. In this work, the apple (Malus × domestica Borkh.), a perennial plant adapted to temperate climates that displays cycles of growth and bud dormancy, was screened for the presence of DAM genes. The candidate genes retrieved were characterized in comparison to DAM genes from other species. Four of them (MdDAM1–4) are structurally very similar to the reported DAM genes. When apple genomic segments containing these candidates were compared to the peach EVERGROWING locus, a highly conserved noncoding region was detected inside their largest intron. Similar sequences were also identified inside introns of apricot and pear DAM genes. Organ expression patterns revealed that MdDAM1–4 are mainly expressed in dormant buds and seeds, with low transcript accumulation in vegetative structures. In addition, the MdDAM genes showed seasonally oscillating patterns of steady-state messenger RNA (mRNA) levels and were downregulated by artificial chilling. Motif analyses in the promoter and in the intronic conserved region of the MdDAM genes disclosed some clues to the regulation of the expression patterns observed. Possible roles for the conserved intronic sequence in dormancy regulation are discussed.
Published: 2016

47. The amounts and dynamics of nitrogen transfer to grasses differ in alfalfa and white clover-based grass-legume mixtures as a result of rooting strategies and rhizodeposit quality

Author: Anne-Sophie Voisin, Gaëtan Louarn, Edina Pereira-Lopès, Joëlle Fustec, François Gastal, Bruno Mary, Paulo César de Faccio Carvalho, Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères (P3F), Institut National de la Recherche Agronomique (INRA), Universidade Federal do Paraná (UFPR), UR LEVA, Ecole Superieure d'Agriculture (ESA), UR 1158 Agroressources et impacts environnementaux, Institut National de la Recherche Agronomique (INRA)-Agroressources et impacts environnementaux (AgroImpact)-Environnement et Agronomie (E.A.)-Biologie et Amélioration des Plantes (BAP), Agroécologie [Dijon], Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), CAPES/Cofecub exchange programme from Brazil (project 684/10), Unité de Recherche Pluridisciplinaire Prairies et Plantes Fourragères ( P3F ), Institut National de la Recherche Agronomique ( INRA ), Universidade Federal do Paraná ( UFPR ), Ecole Superieure d'Agriculture ( ESA ), Institut National de la Recherche Agronomique ( INRA ) -Agroressources et impacts environnementaux ( AgroImpact ) -Environnement et Agronomie ( E.A. ) -Biologie et Amélioration des Plantes ( BAP ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Federal do Rio Grande do Sul [Porto Alegre] ( UFRGS ), and Agroressources et Impacts environnementaux (AgroImpact)
Subjects: [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, [ SDV.BV ] Life Sciences [q-bio]/Vegetal Biology, forage legumes, nitrogen transfer, Perennial plant, Field experiment, Population, Soil Science, chemistry.chemical_element, Plant Science, Biology, Population density, grass-legume mixture, population dynamics, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, education, [ SDV.SA ] Life Sciences [q-bio]/Agricultural sciences, Legume, 2. Zero hunger, education.field_of_study, Plant physiology, food and beverages, Nitrogen, Agronomy, chemistry, nitrogen fixation, Nitrogen fixation, residue quality
Abstract: Differences in the ability to fix and transfer N have been shown between perennial legume species. However, the traits responsible for such variations are largely to be identified. This study aimed at comparing the dynamics of N transfer from alfalfa and white clover and test whether their differences resulted from difference in fixation, legume proportion, population dynamics or tissue composition. [br/]A three-year field experiment and a greenhouse experiment were carried out. Nitrogen fixation and transfer were assessed trough (15) N dilution and difference methods.[br/]Both experiments confirmed significant differences between legumes regarding N transfer capacity. Although alfalfa cumulated twice as much biomass and fixed nitrogen, it transferred smaller amounts of N (59 versus 147 kg N.ha(-1) over three years in the field) under a delayed dynamic. The amounts of nitrogen transferred were related to recent decreases in legume population density. Moreover, root tissue composition differed; white clover had a higher proportion of fine roots with a lower C/N and lignin content. This resulted in more rapid N release from the severed roots of clover.[br/]The traits controlling plant persistency, root material turnover and residue quality may better explain N transfer dynamics than overall legume production and fixation.
Published: 2015
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48. Detection of decreased glomerular filtration rate in intensive care units: serum cystatin C versusserum creatinine

Author: Pierre Delanaye, J. Morel, Pierre Damas, Jean-Marie Krzesinski, Alexandre Lautrette, M. Mehdi, Etienne Cavalier, Christophe Mariat, Guillaume Claisse, Bernard Dubois, Nicolas Maillard, Bernard Lambermont, Centre Hospitalier Universitaire de Liège (CHU-Liège), Service de réanimation chirurgicale, CHU de Saint Etienne, Ecole Nationale d'Agriculture - Meknès, Ecole Nationale d?Agriculture - Meknès, Instituto de Informática da UFRGS (UFRGS), Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Laboratoire de Chimie du Solide, Université de Bordeaux (UB)-Centre National de la Recherche Scientifique (CNRS), Unité de soins intensifs [Clermont Ferrand], CHU Clermont-Ferrand-CHU Gabriel Montpied [Clermont-Ferrand], CHU Clermont-Ferrand, Laboratoire Microorganismes : Génome et Environnement (LMGE), Université Clermont Auvergne [2017-2020] (UCA [2017-2020])-Centre National de la Recherche Scientifique (CNRS), Service de néphrologie et tranplantation, CHU Saint-Etienne-Hôpital nord, Hémostase, Inflammation et sepsis (EA 4609), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS), Hémostase, Inflammation et sepsis, Université Claude Bernard Lyon 1 ( UCBL ), Université de Lyon-Université de Lyon-VetAgro Sup ( VAS ), Instituto de Informática da UFRGS ( UFRGS ), Universidade Federal do Rio Grande do Sul [Porto Alegre] ( UFRGS ), CHU Clermont-Ferrand-Hôpital Gabriel Montpied, and Centre Hospitalier Universitaire de Saint-Etienne [CHU Saint-Etienne] (CHU ST-E)
Subjects: Male, medicine.medical_specialty, Critical Care, Urology, Kidney failure, Renal function, Context (language use), urologic and male genital diseases, Sensitivity and Specificity, behavioral disciplines and activities, [SDV.MP.PRO]Life Sciences [q-bio]/Microbiology and Parasitology/Protistology, chemistry.chemical_compound, Internal medicine, Intensive care, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, medicine, Humans, Cystatin C, ComputingMilieux_MISCELLANEOUS, Creatinine, biology, business.industry, Acute kidney injury, Reproducibility of Results, Acute Kidney Injury, [ SDV.MP.PRO ] Life Sciences [q-bio]/Microbiology and Parasitology/Protistology, medicine.disease, [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology, 3. Good health, Intensive Care Units, Endocrinology, chemistry, Decreased glomerular filtration rate, Nephrology, biology.protein, Female, France, Iohexol, business, Biomarkers, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology, Research Article, Glomerular Filtration Rate, medicine.drug
Abstract: International audience; BACKGROUND: Detecting impaired glomerular filtration rate (GFR) is important in intensive care units (ICU) in order to diagnose acute kidney injuries and adjust the dose of renally excreted drugs. Whether serum Cystatin C (SCysC) may better reflect glomerular filtration rate than serum creatinine (SCr) in the context of intensive care medicine is uncertain. METHODS: We compared the performance of SCysC and SCr as biomarkers of GFR in 47 critically ill patients (median SOFA (Sepsis-related Organ Failure Assessment) score of 5) for whom GFR was measured by a reference method (urinary clearance of iohexol). RESULTS: Mean Iohexol clearance averaged 96 ± 54 mL/min and was under 60 mL/min in 28% of patients. Mean SCr and SCysC concentrations were 0.70 ± 0.33 mg/dL and 1.26 ± 0.61 mg/L, respectively. Area under the ROC curve for a GFR threshold of 60 mL/min was 0.799 and 0.942 for SCr and SCysC, respectively (p = 0.014). CONCLUSIONS: We conclude that ScysC significantly outperfoms SCr for the detection of an impaired GFR in critically ill patients. TRIAL REGISTRATION: ClinicalTrials.gov: B7072006347.
Published: 2014
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49. Genome-wide annotation of the soybean WRKY family and functional characterization of genes involved in response to Phakopsora pachyrhizi infection

Author: Ricardo Luís Mayer Weber, Milena Schenkel Homrich, Atilio Pedro Castagnaro, Beatriz Wiebke-Strohm, Mayra Costa da Cruz Gallo de Carvalho, Francismar Corrêa Marcelino-Guimarães, Caroline Cabreira, Estefania Mancini, Gaston Westergaard, Lauro Bücker-Neto, Renata Stolf, Marta Bencke-Malato, Ricardo Vilela Abdelnoor, Márcia Margis-Pinheiro, Maria Helena Bodanese-Zanettini, Andreia Carina Turchetto-Zolet, Marina Borges Osorio, MARTA BENCKE-MALATO, UFRGS, CAROLINE CABREIRA, UFRGS, BEATRIZ WIEBKE-STROHM, UFRGS, LAURO BÜCKER-NETO, UFRGS, ESTEFANIA MANCINI, Instituto de Agrobiotecnologia Rosario SA, MARINA B. OSORIO, UFRGS, MILENA S. HOMRICH, UFRGS, ANDREIA CARINA TURCHETTO-ZOLET, UFRGS, MAYRA C. C. G. DE CARVALHO, CNPSO - estagiária, RENATA STOLF, CNPSO - estagiària, RICARDO L. M. WEBER, UFRGS, GASTÓN WESTERGAARD, Instituto de Agrobiotecnologia Rosario SA, ATÍLIO P. CASTAGNARO, Estación Experimental Agroindustrial Obispo Colombres (EEAOC), RICARDO VILELA ABDELNOOR, CNPSO, FRANCISMAR CORREA MARCELINO-GUIMARÃES, CNPSO, MÁRCIA MARGIS-PINHEIRO, UFRGS, and MARIA HELENA BODANESE-ZANETTINI, UFRGS.
Subjects: Soja, Otras Biotecnología Agropecuaria, Glycine max, Plant Science, Genome, Genetic transformation, Gene Expression Regulation, Plant, FUNCTIONAL ANALYSIS, FUNGUS RESISTANCE, Genotype, Plant Proteins, Genetics, purl.org/becyt/ford/4.4 [https], food and beverages, Asian Soybean Rust, GLYCINE MAX, Plants, Genetically Modified, TRANSCRIPTION FACTORS, Phakopsora pachyrhizi, Host-Pathogen Interactions, Disease Susceptibility, Genome, Plant, Research Article, ASIAN SOYBEAN RUST, Pseudogene, Biotecnología Agropecuaria, Molecular Sequence Data, Fungus resistance, Biology, Soybeans, Transformation, Genetic, Botany, Consensus Sequence, Transcription factors, Regeneration, Amino Acid Sequence, Gene Silencing, Gene, Plant Diseases, Functional analysis, Basidiomycota, Gene Expression Profiling, fungi, Molecular Sequence Annotation, biology.organism_classification, GENETIC TRANSFORMATION, WRKY protein domain, Gene expression profiling, Plant Leaves, Transformation (genetics), CIENCIAS AGRÍCOLAS, Sequence Alignment, purl.org/becyt/ford/4 [https]
Abstract: Background: Many previous studies have shown that soybean WRKY transcription factors are involved in the plant response to biotic and abiotic stresses. Phakopsora pachyrhizi is the causal agent of Asian Soybean Rust, one of the most important soybean diseases. There are evidences that WRKYs are involved in the resistance of some soybean genotypes against that fungus. The number of WRKY genes already annotated in soybean genome was underrepresented. In the present study, a genome-wide annotation of the soybean WRKY family was carried out and members involved in the response to P. pachyrhizi were identified.Results: As a result of a soybean genomic databases search, 182 WRKY-encoding genes were annotated and 33 putative pseudogenes identified. Genes involved in the response to P. pachyrhizi infection were identified using superSAGE, RNA-Seq of microdissected lesions and microarray experiments. Seventy-five genes were differentially expressed during fungal infection. The expression of eight WRKY genes was validated by RT-qPCR. The expression of these genes in a resistant genotype was earlier and/or stronger compared with a susceptible genotype in response to P. pachyrhizi infection. Soybean somatic embryos were transformed in order to overexpress or silence WRKY genes. Embryos overexpressing a WRKY gene were obtained, but they were unable to convert into plants. When infected with P. pachyrhizi, the leaves of the silenced transgenic line showed a higher number of lesions than the wild-type plants.Conclusions: The present study reports a genome-wide annotation of soybean WRKY family. The participation of some members in response to P. pachyrhizi infection was demonstrated. The results contribute to the elucidation of gene function and suggest the manipulation of WRKYs as a strategy to increase fungal resistance in soybean plants. Fil: Bencke Malato, Marta. Universidade Federal do Rio Grande do Sul; Brasil Fil: Cabreira, Caroline. Universidade Federal do Rio Grande do Sul; Brasil Fil: Wiebke Strohm, Beatriz. Universidade Federal do Rio Grande do Sul; Brasil Fil: Bücker Neto, Lauro. Universidade Federal do Rio Grande do Sul; Brasil Fil: Mancini, Estefania. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Rosario. Instituto de Agrobiotecnología de Rosario; Argentina Fil: Osorio, Marina B.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Homrich, Milena S.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Turchetto Zolet, Andreia C.. Universidade Federal do Rio Grande do Sul; Brasil Fil: De Carvalho, Mayra C.C.G.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Stolf, Renata. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Weber, Ricardo L.M.. Universidade Federal do Rio Grande do Sul; Brasil Fil: Westergaard, Gastón. Instituto de Agrobiotecnología de Rosario (indear); Argentina Fil: Castagnaro, Atilio Pedro. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Tucumán. Instituto de Tecnología Agroindustrial del Noroeste Argentino. Provincia de Tucumán. Ministerio de Desarrollo Productivo. Estación Experimental Agroindustrial ; Argentina Fil: Abdelnoor, Ricardo V.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Marcelino Guimarães, Francismar C.. Ministerio da Agricultura Pecuaria e Abastecimento de Brasil. Empresa Brasileira de Pesquisa Agropecuaria; Brasil Fil: Margis Pinheiro, Márcia. Universidade Federal do Rio Grande do Sul; Brasil Fil: Bodanese Zanettini, Maria H.. Universidade Federal do Rio Grande do Sul; Brasil
Published: 2014

50. Dose-dependent response of nitrogen stable isotope ratio to proportion of legumes in diet to authenticate lamb meat produced from legume-rich diets

Author: S. Prache, Carlos Nabinger, Thais Devincenzi, O. Delfosse, D. Andueza, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Fractionnement des AgroRessources et Environnement - UMR-A 614 (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA)-SFR Condorcet, Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS), Unité Mixte de Recherches sur les Herbivores - UMR 1213 (UMRH), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA), Fractionnement des AgroRessources et Environnement (FARE), Université de Reims Champagne-Ardenne (URCA)-Institut National de la Recherche Agronomique (INRA), Unité Mixte de Recherche sur les Herbivores - UMR 1213 (UMRH), Institut National de la Recherche Agronomique (INRA)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Universidade Federal do Rio Grande do Sul [Porto Alegre] ( UFRGS ), Fractionnement des AgroRessources et Environnement - UMR-A 614 ( FARE ), Université de Reims Champagne-Ardenne ( URCA ) -Institut National de la Recherche Agronomique ( INRA ) -SFR Condorcet, Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Centre National de la Recherche Scientifique ( CNRS ) -Université de Reims Champagne-Ardenne ( URCA ) -Université de Picardie Jules Verne ( UPJV ) -Centre National de la Recherche Scientifique ( CNRS ), Unité Mixte de Recherches sur les Herbivores ( UMR 1213 Herbivores ), VetAgro Sup ( VAS ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique ( INRA ), VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Institut National de la Recherche Agronomique (INRA)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement, Université de Reims Champagne-Ardenne (URCA)-SFR Condorcet, and Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Centre National de la Recherche Scientifique (CNRS)-Institut National de la Recherche Agronomique (INRA)
Subjects: Chimie thérapeutique, [ SDV.AEN ] Life Sciences [q-bio]/Food and Nutrition, animal diseases, [CHIM.THER]Chemical Sciences/Medicinal Chemistry, luzerne, Pasture, isotope stable, Analytical Chemistry, spectrométrie de masse, Grazing, Legume, delta N-15, 2. Zero hunger, alimentation, geography.geographical_feature_category, fourrage, Stable isotope ratio, Discriminant Analysis, food and beverages, [ CHIM.THER ] Chemical Sciences/Medicinal Chemistry, Fabaceae, General Medicine, Sheep and goats, Isotopes of nitrogen, régime alimentaire, Alimentation et Nutrition, authentification, Animal Nutritional Physiological Phenomena, proportion, azote de rapport isotopique stable, Quality Control, Meat, Animal feed, Forage, Biology, légumineuse, muscle long dorsal, Animal science, parasitic diseases, Food and Nutrition, Animals, viande d'agneau, geography, Sheep, Nitrogen Isotopes, performance animale, Animal Feed, Agronomy, Medicinal Chemistry, [SDV.AEN]Life Sciences [q-bio]/Food and Nutrition, Food Science
Abstract: This study investigated the dose-dependent response in lamb meat of stable nitrogen isotope ratio to the dietary proportion of legumes, and the ability of the nitrogen isotope signature of the meat to authenticate meat produced from legume-rich diets. Four groups of nine male Romane lambs grazing a cocksfoot pasture were supplemented with different levels of fresh alfalfa forage to obtain four dietary proportions of alfalfa (0%, 25%, 50% and 75%) for 98 days on average before slaughter (groups L0, L25, L50 and L75). We measured the stable nitrogen isotope ratio in the forages and in the longissimus thoracis muscle. The δ(15)N value of the meat decreased linearly with the dietary proportion of alfalfa. The distribution of the δ(15)N values of the meat discriminated all the L0 lambs from the L75 lambs, and gave a correct classification score of 85.3% comparing lambs that ate alfalfa with those that did not.
Published: 2014
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