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3,922 results on '"Universidade de São Paulo (USP)"'

1. Ethylene Signaling Causing Tolerance of Arabidopsis thaliana Roots to Low pH Stress is Linked to Class III Peroxidase Activity

Author: Christophe Dunand, Victor Alexandre Vitorello, Christian Chervin, Elisabeth Jamet, Lázaro Eustáquio Pereira Peres, Jonathas Pereira Graças, Joni Esrom Lima, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut national de recherche pour l'agriculture, l'alimentation et l'environnement - INRAE (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Universidade Federal de Minas Gerais - UFMG (BRAZIL), Universidade de São Paulo - USP (BRAZIL), Laboratoire de Recherche en Sciences Végétales - LRSV (Castanet-Tolosan, France), Escola Superior de Agricultura 'Luiz de Queiroz' (ESALQ), Universidade de São Paulo (USP), Federal University of Minas Gerais (UFMG), Dynamique et Evolution des Parois cellulaires végétales, Laboratoire de Recherche en Sciences Végétales (LRSV), Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), Centro de Energia Nuclear na Agricultura (CENA ), University of São Paulo (USP), Génomique et Biotechnologie des Fruits (GBF), École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), and Programa de Doutorado-Sanduíche no Exterior - Brazilian CAPES (Grant # 88881.135425/2016-01)
Subjects: Cell death, 0106 biological sciences, 0301 basic medicine, Programmed cell death, Ethylene, Arabidopsis thaliana, Ethylene signaling, Biochimie, Biologie Moléculaire, Plant Science, Stress, 01 natural sciences, Cell wall, Soil, 03 medical and health sciences, chemistry.chemical_compound, Ethylène, [SDV.BV]Life Sciences [q-bio]/Vegetal Biology, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Peroxidase, chemistry.chemical_classification, biology, pH, Acidity, PEROXIDASE, Class III peroxidase, Plant physiology, biology.organism_classification, Roots, Cell wall stiffening, Salicylhydroxamic acid, 030104 developmental biology, Enzyme, Root, chemistry, biology.protein, Biophysics, Agronomy and Crop Science, 010606 plant biology & botany
Abstract: International audience; One irreversible consequence of acidic pH for roots is cell death. Growing evidence suggests the role of hormones and cell wall-related enzymes in response to acidic pH that could possibly avoid cell mortality. Here, we have investigated the role of ethylene and class III peroxidases (CIII Prxs) activity on sensitivity to further low pH treatment. Seedlings of Arabidopsis thaliana were pretreated with ethylene, at various concentrations for various times, and then exposed to low pH. In contrast to non-treated roots, roots pretreated with ethylene for 3 h became tolerant to subsequent low pH, with negligible cell mortality in meristematic (MZ), transition (TZ), and early elongation (EZ) zones. This effect of ethylene was time dependent since it was achieved only when seedlings were pre-incubated with ethylene for at least 3 h. This tolerance induced by ethylene was not observed in the gain-of-function mutation etr1-1 (insensitive to ethylene 1–1). Besides, it was prevented by salicylhydroxamic acid (SHAM) which is an inhibitor of CIII Prxs activity. In late EZ, the decrease in cell expansion due to low pH was dependent on both ethylene signaling and a SHAM-sensitive process. The responses mediated by ethylene signaling might involve CIII Prxs-dependent cell wall modifications, leading to tolerance to low pH and arrest in cell expansion during stress.
Published: 2020
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2. The microbial metabolite p-Cresol induces autistic-like behaviors in mice by remodeling the gut microbiota

Author: Joëlle Chabry, R. Costa-Campos, Jean-Marie Launay, Philippe Langella, Laura Martinez-Gili, Nicolas Caramello, Claire Cherbuy, Antonis Myridakis, Jacques Barik, Laetitia Davidovic, Aurélia Bruneau, Julie Le Merrer, Sebastian P. Fernandez, Jacques Callebert, Susana Barbosa, Marc-Emmanuel Dumas, Nicolas Glaichenhaus, Juliette Canaguier, Patricia Bermudez-Martin, Jérôme A.J. Becker, Institut de pharmacologie moléculaire et cellulaire (IPMC), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Université Côte d'Azur (UCA), Physiologie de la reproduction et des comportements [Nouzilly] (PRC), Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Imagerie et cerveau (iBrain - Inserm U1253 - UNIV Tours ), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Imperial College London, Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (GI3M), Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS), McGill University and Genome Quebec Innovation Centre, MICrobiologie de l'ALImentation au Service de la Santé (MICALIS), AgroParisTech-Université Paris-Saclay-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Marqueurs cardiovasculaires en situation de stress (MASCOT (UMR_S_942 / U942)), Institut National de la Santé et de la Recherche Médicale (INSERM)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Université Sorbonne Paris Nord, Fondation FondaMental [Créteil], Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Institut Français du Cheval et de l'Equitation [Saumur]-Université de Tours-Centre National de la Recherche Scientifique (CNRS), Institut du Fer à Moulin, Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lille-Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur de Lille, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), CHU Lille, McGill University = Université McGill [Montréal, Canada], Hôpital Lariboisière-Fernand-Widal [APHP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015 - 2019) (COMUE UCA)-Université Côte d'Azur (UCA), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours, Department of Metabolism, Digestion and Reproduction, National Heart and Lung Institute [London] (NHLI), Royal Brompton and Harefield NHS Foundation Trust-Imperial College London, Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)-Université Côte d'Azur (UCA), Institut Français du Cheval et de l'Equitation [Saumur] (IFCE)-Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Metabolic functional (epi)genomics and molecular mechanisms involved in type 2 diabetes and related diseases - UMR 8199 - UMR 1283 (EGENODIA (GI3M)), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Université Sorbonne Paris Nord, UK Research & Innovation (UKRI) Medical Research Council UK (MRC)MR/M501797/1, Region the Centre-Val de Loire (ARD2020 Biomedicaments GPCRAb), ANR-19-CE14-0024,MICROBIAUTISM,L'axe microbiome-intestin-cerveau dans les troubles du spectre de l'autisme(2019), European Project: 291840,EC:FP7:HEALTH,FP7-ERANET-2011-RTD,ERA-NET NEURON II(2012), Institute of Environmental Assessment and Water Research (IDAEA), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Laboratoire d'Énergétique Moléculaire et Macroscopique, Combustion (EM2C), CentraleSupélec-Centre National de la Recherche Scientifique (CNRS)-Université Paris Saclay (COmUE), Institut de Génétique et de Biologie Moléculaire et Cellulaire (IGBMC), Université de Strasbourg (UNISTRA)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Emerging Methods Section, Environment and Climate Change Canada, FERNANDEZ, SEBASTIAN PABLO, L'axe microbiome-intestin-cerveau dans les troubles du spectre de l'autisme - - MICROBIAUTISM2019 - ANR-19-CE14-0024 - AAPG2019 - VALID, Network of European funding for Neuroscience research - ERA-NET NEURON II - - EC:FP7:HEALTH2012-01-01 - 2015-12-31 - 291840 - VALID, Université Côte d'Azur (UCA), Imperial College London-Royal Brompton and Harefield NHS Foundation Trust, Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT), Commission of the European Communities, and Medical Research Council (MRC)
Subjects: 0301 basic medicine, SYMPTOMS, Autism Spectrum Disorder, Metabolite, Autism, [SDV]Life Sciences [q-bio], CHILDREN, Gut flora, Microbial ecology, DOPAMINE, Cresols, Mice, chemistry.chemical_compound, 0302 clinical medicine, fluids and secretions, 1108 Medical Microbiology, MESH: Fecal Microbiota Transplantation, ANXIETY, p-Cresol, MESH: Animals, SEQUENCE ALIGNMENT, MESH: Autism Spectrum Disorder, 0303 health sciences, SOCIAL REWARD, Microbiota, QR100-130, Cognition, MOUSE MODEL, Fecal Microbiota Transplantation, GASTROINTESTINAL DYSFUNCTION, [SDV] Life Sciences [q-bio], MESH: Cresols, Reward system, Anxiety, [SDV.NEU]Life Sciences [q-bio]/Neurons and Cognition [q-bio.NC], medicine.symptom, Life Sciences & Biomedicine, Neurotypical, 0605 Microbiology, medicine.drug, Microbiology (medical), medicine.medical_specialty, MESH: Autistic Disorder, MU-OPIOID RECEPTOR, Biology, Microbiology, MESH: Gastrointestinal Microbiome, Excretion, 03 medical and health sciences, [CHIM.ANAL]Chemical Sciences/Analytical chemistry, Dopamine, Internal medicine, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], parasitic diseases, medicine, Animals, Humans, [CHIM]Chemical Sciences, Autistic Disorder, MESH: Mice, Feces, SPECTRUM DISORDER, 030304 developmental biology, Behavior, Science & Technology, MESH: Humans, 0602 Ecology, Mechanism (biology), Research, medicine.disease, biology.organism_classification, 4-Cresol, Gastrointestinal Microbiome, Transplantation, 030104 developmental biology, Endocrinology, chemistry, Immunology, 030217 neurology & neurosurgery
Abstract: Background Autism spectrum disorders (ASD) are associated with dysregulation of the microbiota-gut-brain axis, changes in microbiota composition as well as in the fecal, serum, and urine levels of microbial metabolites. Yet a causal relationship between dysregulation of the microbiota-gut-brain axis and ASD remains to be demonstrated. Here, we hypothesized that the microbial metabolite p-Cresol, which is more abundant in ASD patients compared to neurotypical individuals, could induce ASD-like behavior in mice. Results Mice exposed to p-Cresol for 4 weeks in drinking water presented social behavior deficits, stereotypies, and perseverative behaviors, but no changes in anxiety, locomotion, or cognition. Abnormal social behavior induced by p-Cresol was associated with decreased activity of central dopamine neurons involved in the social reward circuit. Further, p-Cresol induced changes in microbiota composition and social behavior deficits could be transferred from p-Cresol-treated mice to control mice by fecal microbiota transplantation (FMT). We also showed that mice transplanted with the microbiota of p-Cresol-treated mice exhibited increased fecal p-Cresol excretion, compared to mice transplanted with the microbiota of control mice. In addition, we identified possible p-Cresol bacterial producers. Lastly, the microbiota of control mice rescued social interactions, dopamine neurons excitability, and fecal p-Cresol levels when transplanted to p-Cresol-treated mice. Conclusions The microbial metabolite p-Cresol induces selectively ASD core behavioral symptoms in mice. Social behavior deficits induced by p-Cresol are dependant on changes in microbiota composition. Our study paves the way for therapeutic interventions targeting the microbiota and p-Cresol production to treat patients with ASD.
Published: 2021
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3. Empirical formulation for multiple groups of primary biological ice nucleating particles from field observations over Amazonia

Author: Vaughan T. J. Phillips, Cindy E. Morris, Paul J. DeMott, Susannah M. Burrows, Sachin Patade, Bettina Weber, Fábio Luiz Teixeira Gonçalves, Christopher Pöhlker, Pierre Amato, Jann Schrod, Heinz Bingemer, Carl Alwmark, Daniel A. Knopf, Paulo Artaxo, Department of Physical Geography and Ecosystem Science [Lund], Lund University [Lund], Institut de Chimie de Clermont-Ferrand (ICCF), Institut de Chimie du CNRS (INC)-Centre National de la Recherche Scientifique (CNRS)-Université Clermont Auvergne (UCA)-Institut national polytechnique Clermont Auvergne (INP Clermont Auvergne), Université Clermont Auvergne (UCA)-Université Clermont Auvergne (UCA), Institute for Atmospheric and Environmental Sciences [Frankfurt/Main] (IAU), Goethe-University Frankfurt am Main, Atmospheric Sciences and Global Change Division, Pacific Northwest National Laboratory (PNNL), Department of Atmospheric Science [Fort Collins], Colorado State University [Fort Collins] (CSU), Departamento de Ciencias Atmosfericas [São Paulo], Instituto de Astronomia, Geofísica e Ciências Atmosféricas [São Paulo] (IAG), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), Institute for Terrestrial and Planetary Atmospheres [Stony Brook] (ITPA), School of Marine and Atmospheric Sciences [Stony Brook] (SoMAS), Stony Brook University [SUNY] (SBU), State University of New York (SUNY)-State University of New York (SUNY)-Stony Brook University [SUNY] (SBU), State University of New York (SUNY)-State University of New York (SUNY), Unité de Pathologie Végétale (PV), Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Department of Geology [Lund], Universidade de São Paulo = University of São Paulo (USP), Multiphase Chemistry Department [Mainz], Max Planck Institute for Chemistry (MPIC), Max-Planck-Gesellschaft-Max-Planck-Gesellschaft, Karl-Franzens-Universität Graz, Swedish Research Council (‘Vetenskapsradet’), German Federal Ministry of Education and Research (BMBF contracts 01LB1001A, 01LK1602A and 01LK1602B), U.S. National Science Foundation Award #1660486, NASA award NNX17AJ12G, Deutsche Forschungsgemeinschaft (DFG) under the Research Unit FOR 1525 (INUIT), Max Planck Society, Brazilian Ministério da Ciência, Tecnologia e Inovação (MCTI/FINEP contract 01.11.01248.00), Amazon State University (UEA), FAPEAM, LBA/INPA, SDS/CEUC/RDS-Uatumã, U.S. Department of Energy, Office of Science (BER), Atmospheric System Research (DE-SC0018929, DE-SC0016370, DE-SC0020006), European Project: 603445,EC:FP7:ENV,FP7-ENV-2013-two-stage,BACCHUS(2013), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Universidade de São Paulo (USP), Karl-Franzens-Universität [Graz, Autriche], and University of Graz
Subjects: [PHYS.PHYS.PHYS-AO-PH]Physics [physics]/Physics [physics]/Atmospheric and Oceanic Physics [physics.ao-ph], Atmospheric Science, Detritus, 010504 meteorology & atmospheric sciences, Atmospheric models, biology, Amazon rainforest, medicine.disease_cause, Atmospheric sciences, biology.organism_classification, 01 natural sciences, Soot, 010305 fluids & plasmas, Aerosol, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Algae, FLORESTAS TROPICAIS, Pollen, 0103 physical sciences, medicine, Ice nucleus, Environmental science, [MATH]Mathematics [math], 0105 earth and related environmental sciences, Amazonie
Abstract: To resolve the various types of biological ice nuclei (IN) with atmospheric models, an extension of the empirical parameterization (EP) (Phillips et al. 2008; 2013) is proposed to predict the active IN from multiple groups of primary biological aerosol particles (PBAPs). Our approach is to utilize coincident observations of PBAP sizes, concentrations, biological composition, and ice-nucleating ability. The parameterization organizes the PBAPs into five basic groups: fungal spores, bacteria, pollen, viral particles, plant/animal detritus, algae, and their respective fragments. This new biological component of the EP was constructed by fitting predicted concentrations of PBAP IN to those observed at the Amazon Tall Tower Observatory (ATTO) site located in the central Amazon. The fitting parameters for pollen and viral particles, plant/animal detritus, which are much less active as IN than fungal and bacterial groups, are constrained based on their ice nucleation activity from the literature. The parameterization has empirically derived dependencies on the surface area of each group (except algae), and the effects of variability in their mean sizes and number concentrations are represented via their influences on the surface area. The concentration of active algal IN is estimated from literature-based measurements.Predictions of this new biological component of the EP are consistent with previous laboratory and field observations not used in its construction. The EP scheme was implemented in a 0D parcel model. It confirms that biological IN account for most of the total IN activation at temperatures warmer than −20°C and at colder temperatures dust and soot become increasingly more important to ice nucleation.
Published: 2021
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4. Glossary on atmospheric electricity and its effects on biology

Author: Lluis M. Mir, Marko Vana, Oscar van der Velde, Michal Cifra, Snezana Dragovic, Michael J. Rycroft, Pablo Fdez-Arroyabe, Susana Barbosa, James C Matthews, Christos Haldoupis, Milan Djordjevic, Konstantinos Kourtidis, Christina Oikonomou, Ashot Chilingarian, Snezana Savoska, Pavlos Kassomenos, Jens Olaf Pepke Pedersen, Marius-Victor Birsan, Dominic Royé, Anna Odzimek, Francesca Apollonio, Colin Price, H. Mkrtchyan, Ellard R. Hunting, Christos Consoulas, Amparo López-Jiménez, Daniel Robert, József Bór, Xuemeng Chen, Ciro Salcines, Michel Vorenhout, Benedicto Crespo-Facorro, Reik V. Donner, Department of Physics [Heraklion], University of Crete [Heraklion] (UOC), Vectorologie et thérapeutiques anti-cancéreuses [Villejuif] (UMR 8203), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Aspects métaboliques et systémiques de l'oncogénèse pour de nouvelles approches thérapeutiques (METSY), Institut Gustave Roussy (IGR)-Université Paris-Saclay-Centre National de la Recherche Scientifique (CNRS), Institute of Photonics and Electronics of the Czech Academy of Sciences (UFE / CAS), Czech Academy of Sciences [Prague] (CAS), Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), University of Helsinki, Europe, European Cooperation in Science and Technology, Ministry of Science and Higher Education (Poland), and Polish Academy of Sciences
Subjects: INGENIERIA HIDRAULICA, Atmospheric Science, 010504 meteorology & atmospheric sciences, Glossary, Process (engineering), Health, Toxicology and Mutagenesis, [SDV]Life Sciences [q-bio], Biological effects, Electronet, Atmospheric electricity phenomena, 01 natural sciences, Biometeorological profile, 03 medical and health sciences, [SPI]Engineering Sciences [physics], 0302 clinical medicine, Electricity, Interdisciplinary, Multidisciplinary approach, Atmospheric electric fields, 13.- Tomar medidas urgentes para combatir el cambio climático y sus efectos, Biology, Scientific disciplines, 0105 earth and related environmental sciences, 030203 arthritis & rheumatology, Ecology, COST Action 15211, Atmospheric electric field, Data science, Work (electrical), 13. Climate action, [SDE]Environmental Sciences, Atmospheric electricity, Transdisciplinary
Abstract: [EN] There is an increasing interest to study the interactions between atmospheric electrical parameters and living organisms at multiple scales. So far, relatively few studies have been published that focus on possible biological effects of atmospheric electric and magnetic fields. To foster future work in this area of multidisciplinary research, here we present a glossary of relevant terms. Its main purpose is to facilitate the process of learning and communication among the different scientific disciplines working on this topic. While some definitions come from existing sources, other concepts have been re-defined to better reflect the existing and emerging scientific needs of this multidisciplinary and transdisciplinary area of research., This paper is based upon work from the COST Action "Atmospheric Electricity Network: coupling with the Earth System, climate and biological systems (ELECTRONET)," supported by COST (European Cooperation in Science and Technology). AO received funding from Poland Ministry of Science and Higher Education for statutory research of the Institute of Geophysics, Polish Academy of Sciences (Grant No 3841/E-41/S/2019).
Published: 2020
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5. Intratumoral Immunization by p19Arf and Interferon-β Gene Transfer in a Heterotopic Mouse Model of Lung Carcinoma

Author: Eugenia Costanzi-Strauss, Daniela B Zanatta, Ruan F.V. Medrano, Bryan E. Strauss, Guido Kroemer, João Paulo Portela Catani, Sandy Adjemian, Aline Hunger, Paulo Roberto Del Valle, Instituto do Câncer do Estado = Cancer Institute of the State of São Paulo (ICESP), Universidade de São Paulo (USP), Plateforme de métabolomique, Direction de la recherche [Gustave Roussy], Institut Gustave Roussy (IGR)-Institut Gustave Roussy (IGR), Centre de Recherche des Cordeliers (CRC), Université Paris Diderot - Paris 7 (UPD7)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut Gustave Roussy (IGR), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Karolinska University Hospital [Stockholm], Cancer Institute of Sao Paulo, Universidade de São Paulo ( USP ), Institut Gustave Roussy ( IGR ) -Institut Gustave Roussy ( IGR ), Centre de Recherche des Cordeliers ( CRC ), Université Paris Diderot - Paris 7 ( UPD7 ) -École pratique des hautes études ( EPHE ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ), Institut Gustave Roussy ( IGR ), Hôpital Européen Georges Pompidou [APHP] ( HEGP ), Universidade de São Paulo = University of São Paulo (USP), Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Paris Diderot - Paris 7 (UPD7)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), HAL UPMC, Gestionnaire, and Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)
Subjects: 0301 basic medicine, Cancer Research, medicine.medical_treatment, Genetic enhancement, CCL3, [SDV.CAN]Life Sciences [q-bio]/Cancer, Biology, lcsh:RC254-282, [ SDV.CAN ] Life Sciences [q-bio]/Cancer, 03 medical and health sciences, 0302 clinical medicine, Immune system, [SDV.CAN] Life Sciences [q-bio]/Cancer, medicine, Carcinoma, Cisplatin, Immunotherapy, lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens, medicine.disease, 3. Good health, 030104 developmental biology, Oncology, Tumor progression, 030220 oncology & carcinogenesis, Immunology, Immunogenic cell death, medicine.drug
Abstract: International audience; Therapeutic strategies that act by eliciting and enhancing antitumor immunity have been clinically validated as an effective treatment modality but may benefit from the induction of both cell death and immune activation as primary stimuli. Using our AdRGD-PG adenovector platform, we show here for the first time that in situ gene transfer of p19Arf and interferon-β (IFNβ) in the LLC1 mouse model of lung carcinoma acts as an immunotherapy. Although p19Arf is sufficient to induce cell death, only its pairing with IFNβ significantly induced markers of immunogenic cell death. In situ gene therapy with IFNβ, either alone or in combination with p19Arf, could retard tumor progression, but only the combined treatment was associated with a protective immune response. Specifically in the case of combined intratumoral gene transfer, we identified 167 differentially expressed genes when using microarray to evaluate tumors that were treated in vivo and confirmed the activation of CCL3, CXCL3, IL1α, IL1β, CD274, and OSM, involved in immune response and chemotaxis. Histologic evaluation revealed significant tumor infiltration by neutrophils, whereas functional depletion of granulocytes ablated the antitumor effect of our approach. The association of in situ gene therapy with cisplatin resulted in synergistic elimination of tumor progression. In all, in situ gene transfer with p19Arf and IFNβ acts as an immunotherapy involving recruitment of neutrophils, a desirable but previously untested outcome, and this approach may be allied with chemotherapy, thus providing significant antitumor activity and warranting further development for the treatment of lung carcinoma.
Published: 2016
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6. Dataset of differentially regulated proteins in HUVECs challenged with wild type and UGM1 mutant Aspergillus fumigatus strains

Author: Marcel Lyra Cunha, Gustavo H.M.F. Souza, Nathalia Curty, Gabriela W. P. Neves, Thierry Fontaine, Paula H. Kubitschek-Barreira, Anne Beauvais, Leila M. Lopes-Bezerra, Gustavo H. Goldman, Jean-Paul Latgé, Universidade do Estado do Rio de Janeiro [Rio de Janeiro] (UERJ), Aspergillus, Institut Pasteur [Paris], MS Applications Research and Development Laboratory, Waters Corporation, Waters Technologies Brazil, Faculdade de Ciências Farmacêuticas de Ribeirão Preto [São Paulo], Universidade de São Paulo (USP), This work was financially supported through grants from the Fundação Carlos Chagas de Amparo à Pesquisa do Estado do Rio de Janeiro, Brazil (FAPERJ), grants E-26/100.329/2014 and E-26/102819/2012. LMLB is a research fellow of Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) and Faperj, and GWPN is a PhD Nota 10 fellow of FAPERJ., Universidade do Estado do Rio de Janeiro [Rio de Janeiro] ( UERJ ), Universidade de São Paulo ( USP ), Institut Pasteur [Paris] (IP), and Universidade de São Paulo = University of São Paulo (USP)
Subjects: 0301 basic medicine, [SDV]Life Sciences [q-bio], Galactosaminogalactan, Mutant, Context (language use), Aspergillosis, lcsh:Computer applications to medicine. Medical informatics, Microbiology, Aspergillus fumigatus, 03 medical and health sciences, chemistry.chemical_compound, HUVEC, medicine, lcsh:Science (General), Pathogen, UGM1 mutant, [ SDV.MP.MYC ] Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, [SDV.MP.MYC]Life Sciences [q-bio]/Microbiology and Parasitology/Mycology, Data Article, GAG, Multidisciplinary, 030102 biochemistry & molecular biology, biology, [ SDV ] Life Sciences [q-bio], Wild type, medicine.disease, biology.organism_classification, PROTEOME, 030104 developmental biology, chemistry, Proteome, Invasive aspergillosis, lcsh:R858-859.7, lcsh:Q1-390
Abstract: International audience; Invasive aspergillosis is the primary opportunistic invasive fungal infection described in neutropenic hematologic patients, caused by the angioinvasive pathogen Aspergillus fumigatus. The molecular mechanisms associated with A. fumigatus infection in the vascular endothelium are poorly understood. In this context, we used a high-throughput proteomic approach to unveil the proteins modulated in HUVECs after interaction with a wild type strain and the UGM1 mutant (Δugm1) of A. fumigatus. The proteomic analysis was also performed in HUVECs challenged with a galactosaminogalactan (GAG) purified from A. fumigatus cell wall. The dataset presented here correspond to all proteins identified that fit a 2-fold change criteria (log 2 ratio ≥ 1 or ≤ -1), disregarding the statistical validation cut off, in order to supplement the research article entitled "Modifications to the composition of the hyphal outer layer of Aspergillus fumigatus modulates the HUVEC proteins associated with inflammatory and stress responses" (G.W.P. Neves, N.A. Curty, P.H. Kubitschek-Barreira, T. Fontaine, G.H.M.F. Souza, M. Lyra Cunha, G.H. Goldman, A. Beauvais, J.P. Latgé, L.M. Lopes-Bezerra, 2016) [1]. The mass spectrometry proteomic data have been deposited in the ProteomeXchange Consortium via the PRIDE partner repository with the dataset identifier PRIDE: PXD002823.
Published: 2016
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7. An integrated analysis of mRNA and sRNA transcriptional profiles in Coffea arabica L. roots: insights on nitrogen starvation responses

Author: Juliana Costa Silva, Inácio L.M. Junqueira-de-Azevedo, Anderson R. Meda, Luiz Filipe Protasio Pereira, Joni Esrom Lima, Alexandre Rossi Paschoal, Viviane Yumi Baba, Silvia Graciele Hülse de Souza, Romain Guyot, Paulo Mazzafera, Osvaldo Reis Junior, João Danillo Moura Soares, Tiago Benedito dos Santos, Ursula Castro de Oliveira, Antonio Figueira, Suzana Tiemi Ivamoto, Alan P. R. Lorenzetti, Milton Yutaka Nishiyama Junior, Douglas Silva Domingues, João Benhur Mokochinski, Luiz Gonzaga Esteves Vieira, Universidade de São Paulo (USP), Universidade Federal de Minas Gerais [Belo Horizonte] (UFMG), Universidade Tecnológica Federal do Paraná [Curitiba] (UTFPR), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), State University of Londrina = Universidade Estadual de Londrina, Instituto Butantan [São Paulo], Universidade Estadual de Campinas (UNICAMP), UMR - Interactions Plantes Microorganismes Environnement (UMR IPME), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud]), Inst Agron Parana, Univ Oeste Paulista, Universidade Federal de Minas Gerais (UFMG), Univ Tecnol Fed Parana, Universidade Estadual Paulista (Unesp), Univ Paranaense, Universidade Estadual de Londrina (UEL), Inst Butantan, COFFEEADAPT, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), Tiago Benedito dos Santos, Universidade do Oeste Paulista, João D. M. Soares, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR, Joni E. Lima, Departamento de Botânica/Instituto de Ciências Biológicas/Universidade Federal de Minas Gerais - UFMG, Juliana C. Silva, Programa de pós-graduação em Bioinformática/Universidade Tecnológica Federal do Paraná, Suzana T. Ivamoto, Departamento de Botânica/Instituto de Biociências de Rio Claro/Universidade Estadual Paulista, Viviane Y. Baba, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR, Silvia G. H. Souza, Laboratório de Biologia Molecular/Universidade Paranaense, Alan P. R. Lorenzetti, Programa de Pós-graduação em Genética e Biologia Molecular/Universidade Estadual de Londrina - UEL, Alexandre R. Paschoal, Programa de pós-graduação em Bioinformática/Universidade Tecnológica Federal do Paraná, Anderson R. Meda, Laboratório de Biotecnologia Vegetal, Instituto Agronômico do Paraná - IAPAR, Milton Y. Nishiyama Júnior, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan, Úrsula C. de Oliveira, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan, João B. Mokochinski, Departamento de Biologia Vegetal, Instituto de Biologia/Universidade Estadual de Campinas, Romain Guyot, IRD, UMR IPME, COFFEEADAPT, Inácio L. M. Junqueira-de-Azevedo, Laboratório Especial de Toxinologia Aplicada/Instituto Butantan, Antônio V. O. Figueira, Centro de Energia Nuclear na Agricultura/Universidade de São Paulo - USP, Paulo Mazzafera, Programa de Pós-graduação em Genética e Biologia Molecular/Universidade Estadual de Londrina - UEL, Osvaldo R. Júnior, Life Sciences Core Facility - LaCTAD/Universidade Estadual de Campinas, Luiz G. E. Vieira, Universidade do Oeste Paulista, LUIZ FILIPE PROTASIO PEREIRA, CNPCa, and Douglas S. Domingues, Departamento de Botânica/Instituto de Biociências de Rio Claro/Universidade Estadual Paulista.
Subjects: 0106 biological sciences, 0301 basic medicine, Small RNA, Nitrogen, Coffea, RNA-Seq, Biology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Plant Roots, 01 natural sciences, Coffee, Transcriptome, 03 medical and health sciences, Gene Expression Regulation, Plant, Stress, Physiological, Ammonium Compounds, Genetics, RNA, Messenger, Amino Acids, Gene, Differential gene expression, 2. Zero hunger, Nitrates, microRNA, Coffea arabica, High-Throughput Nucleotide Sequencing, EXPRESSÃO GÊNICA, RNA, Molecular Sequence Annotation, MicroRNA, General Medicine, Plant Leaves, MicroRNAs, [SDV.BV.AP]Life Sciences [q-bio]/Vegetal Biology/Plant breeding, Gene Ontology, 030104 developmental biology, MRNA Sequencing, Biochemistry, RNA, Plant, Seeds, Transfer RNA, RNA, Small Untranslated, Nitrogen transport, RNA-seq, Poly A, 010606 plant biology & botany
Abstract: Made available in DSpace on 2019-10-04T12:33:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2019-01-01 Brazilian Coffee Research Consortium Fundacao Araucaria Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coffea arabica L. is an important agricultural commodity, accounting for 60% of traded coffee worldwide. Nitrogen (N) is a macronutrient that is usually limiting to plant yield; however, molecular mechanisms of plant acclimation to N limitation remain largely unknown in tropical woody crops. In this study, we investigated the transcriptome of coffee roots under N starvation, analyzing poly-A+ libraries and small RNAs. We also evaluated the concentration of selected amino acids and N-source preferences in roots. Ammonium was preferentially taken up over nitrate, and asparagine and glutamate were the most abundant amino acids observed in coffee roots. We obtained 34,654 assembled contigs by mRNA sequencing, and validated the transcriptional profile of 12 genes by RT-qPCR. Illumina small RNA sequencing yielded 8,524,332 non-redundant reads, resulting in the identification of 86 microRNA families targeting 253 genes. The transcriptional pattern of eight miRNA families was also validated. To our knowledge, this is the first catalog of differentially regulated amino acids, N sources, mRNAs, and sRNAs in Arabica coffee roots. Inst Agron Parana, Lab Biotecnol Vegetal, BR-86047902 Londrina, Brazil Univ Oeste Paulista, Rodovia Raposo Tavares Km 572, BR-19067175 Presidente Prudente, Brazil Univ Sao Paulo, Ctr Energia Nucl Agr, BR-13400970 Piracicaba, Brazil Univ Fed Minas Gerais, Inst Ciencias Biol, Dept Bot, BR-31270901 Belo Horizonte, MG, Brazil Univ Tecnol Fed Parana, Programa Posgrad Bioinformat, BR-86300000 Cornelio Procopio, Brazil Univ Estadual Paulista, Inst Biociencias Rio Claro, Dept Bot, BR-13506900 Rio Claro, Brazil Univ Paranaense, Lab Biol Mol, BR-87502210 Umuarama, Brazil Univ Estadual Londrina, Programa Posgrad Genet & Biol Mol, BR-86057970 Londrina, Brazil Inst Butantan, Lab Especial Toxinol Aplicada, BR-05503900 Sao Paulo, Brazil Univ Estadual Campinas, Inst Biol, Dept Biol Vegetal, BR-13083970 Campinas, SP, Brazil COFFEEADAPT, UMR IPME, IRD, BP 64501, F-34394 Montpellier 5, France Univ Estadual Campinas, Life Sci Core Facil LaCTAD, BR-13083886 Campinas, SP, Brazil Embrapa Cafe, BR-70770901 Brasilia, DF, Brazil Univ Estadual Paulista, Inst Biociencias Rio Claro, Dept Bot, BR-13506900 Rio Claro, Brazil CAPES: PVE 084/2013
Published: 2019
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8. Cytotoxic anti-circumsporozoite antibodies target malaria sporozoites in the host skin

Author: Silvia Beatriz Boscardin, Tomoko Ishino, Marcio Massao Yamamoto, Olivier Silvie, Rogerio Amino, Sylvie Dartevelle, François Traincard, Masao Yuda, Raquel Hoffmann Panatieri, Joana Tavares, Eduardo Aliprandini, Sabine Thiberge, Infection et Immunité paludéennes - Malaria Infection and Immunity, Institut Pasteur [Paris] (IP), Instituto de Investigação e Inovação em Saúde (I3S), Universidade do Porto = University of Porto, Instituto de Biologia Molecular e Celular - institute for molecular and cell biology [Porto, Portugal] (IBMC), Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), Centre de Production et Infection des Anophèles (plateforme) - Center for the Production and Infection of Anopheles (platform) (CEPIA), Centre d'Immunologie et des Maladies Infectieuses (CIMI), Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS), Ehime University [Matsuyama, Japon], Mie University Graduate School / Faculty of Medicine [Japan], Mie University, Ingénierie des Anticorps (plate-forme) - Antibody Engineering (Platform), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), This work was supported by funds from Institut Pasteur, Paris, the French National Research Agency (grant no. ANR-14-CE16-Im3alaria), the French Government’s ‘Investissement d’Avenir’ program, Laboratoire d’Excellence ‘Integrative Biology of Emerging Infectious Diseases’ (grant no. ANR-10-LABX-62-IBEID) and ‘ParaFrap’ (grant no. ANR-11-LABX-0024), the São Paulo Research Foundation (FAPESP, grant no. 2014/50631-0), the National Council for Scientific and Technological Development (CNPq), the BNP Paribas CIB, the Portuguese Science and Technology Foundation (FCT, grant no. IF/00881/2012/CP0158) and the European Social Fund (Human Potential Operational Programme)., We thank the team of the Centre of Production and Infection of Anopheles, Institut Pasteur, in particular M. Szatanik, C. Thouvenot, S. Golba, J. Pham and A. Lorthiois for providing mosquitoes and P. falciparum SPZs, the team of the Platform of Dynamic Imaging, Institut Pasteur, in particular Dr S. Shorte and M.-A. Nicola for the access to the confocal microscopes and IVIS system, Dr K. Kim from the Albert Einstein College of Medicine for providing the P. yoelii YM GFP-luciferase, Dr V. Nussenzweig from New York University for providing the P. berghei and falciparumnized P. berghei parasites, Dr M. Soares from the Instituto Gulbenkian for providing the JHT−/− mice, Dr P.-M. Lledo and Dr P. Bruhns from the Institut Pasteur for providing, respectively, the C3−/− and the FcRγ−/− mice, the team of the Clinical Investigation and Access to BioResources, in particular M.-N. Ungeheuer for providing the erythrocytes for the P. falciparum culture, Dr P. Baldacci and Dr P. Formaglio for their critical reading of the manuscript., ANR-14-CE16-0030,IM3alaria,Imagerie et amélioration de la protection immunitaire contre le parasite responsable du paludisme.(2014), ANR-10-LABX-0062,IBEID,Integrative Biology of Emerging Infectious Diseases(2010), ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011), Amino, Rogerio, Appel à projets générique - Imagerie et amélioration de la protection immunitaire contre le parasite responsable du paludisme. - - IM3alaria2014 - ANR-14-CE16-0030 - Appel à projets générique - VALID, Integrative Biology of Emerging Infectious Diseases - - IBEID2010 - ANR-10-LABX-0062 - LABX - VALID, Laboratoires d'excellence - Alliance française contre les maladies parasitaires - - ParaFrap2011 - ANR-11-LABX-0024 - LABX - VALID, Institut Pasteur [Paris], Universidade do Porto, Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Centre d'Immunologie et de Maladies Infectieuses (CIMI), Ehime University [Matsuyama], and Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS)
Subjects: 0301 basic medicine, MESH: Antibodies, Protozoan/pharmacology, Plasmodium berghei, [SDV]Life Sciences [q-bio], Fc receptor, Protozoan Proteins, MESH: Antibodies, Monoclonal/pharmacology, Antibodies, Protozoan, Applied Microbiology and Biotechnology, MESH: Cell Movement/drug effects, Mice, MESH: Protozoan Proteins/immunology, Cell Movement, MESH: Animals, MESH: Plasmodium falciparum/immunology, Skin, MESH: Pore Forming Cytotoxic Proteins/metabolism, MESH: Sporozoites/cytology, biology, Antibodies, Monoclonal, 3. Good health, Circumsporozoite protein, MESH: Sporozoites/immunology, [SDV] Life Sciences [q-bio], [SDV.IMM.IA]Life Sciences [q-bio]/Immunology/Adaptive immunology, Sporozoites, MESH: Skin/parasitology, MESH: Plasmodium berghei/immunology, [SDV.IMM.IA] Life Sciences [q-bio]/Immunology/Adaptive immunology, MESH: Plasmodium yoelii/cytology, MESH: Malaria/immunology, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Antibody, Plasmodium yoelii, Microbiology (medical), Pore Forming Cytotoxic Proteins, [SDV.IMM] Life Sciences [q-bio]/Immunology, medicine.drug_class, Immunology, Plasmodium falciparum, MESH: Plasmodium yoelii/immunology, Monoclonal antibody, complex mixtures, Microbiology, 03 medical and health sciences, parasitic diseases, Genetics, medicine, Animals, MESH: Mice, fungi, Cell Biology, biology.organism_classification, Malaria, 030104 developmental biology, Culicidae, Humoral immunity, biology.protein, MESH: Culicidae, MESH: Female
Abstract: The circumsporozoite protein (CSP) is the major surface protein of malaria sporozoites (SPZs), the motile and invasive parasite stage inoculated in the host skin by infected mosquitoes. Antibodies against the central CSP repeats of different plasmodial species are known to block SPZ infectivity1–5, but the precise mechanism by which these effectors operate is not completely understood. Here, using a rodent Plasmodium yoelii malaria model, we show that sterile protection mediated by anti-P. yoelii CSP humoral immunity depends on the parasite inoculation into the host skin, where antibodies inhibit motility and kill P. yoelii SPZs via a characteristic ‘dotty death’ phenotype. Passive transfer of an anti-repeat monoclonal antibody (mAb) recapitulates the skin inoculation-dependent protection, in a complement- and Fc receptor γ-independent manner. This purified mAb also decreases motility and, notably, induces the dotty death of P. yoelii SPZs in vitro. Cytotoxicity is species-transcendent since cognate anti-CSP repeat mAbs also kill Plasmodium berghei and Plasmodium falciparum SPZs. mAb cytotoxicity requires the actomyosin motor-dependent translocation and stripping of the protective CSP surface coat, rendering the parasite membrane susceptible to the SPZ pore-forming-like protein secreted to wound and traverse the host cell membrane6. The loss of SPZ fitness caused by anti-P. yoelii CSP repeat antibodies is thus a dynamic process initiated in the host skin where SPZs either stop moving7, or migrate and traverse cells to progress through the host tissues7–9 at the eventual expense of their own life. In a rodent malaria model, antibodies against the CSP protein that coats sporozoites lead to Plasmodium yoelii killing in the skin in a process that involves stripping off the CSP coat, rendering parasites susceptible to pore-forming-like proteins.
Published: 2018
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9. Canonical PI3Kγ signaling in myeloid cells restricts Trypanosoma cruzi infection and dampens chagasic myocarditis

Author: Thiago M. Cunha, Lucas Esteves Cardozo, Florêncio Figueiredo, Konstantina Lyroni, Emilio Hirsch, F. N. Gava, João Santana da Silva, Edecio Cunha-Neto, Renata Sesti-Costa, Christophe Chevillard, Carla D. Lopes, Maria R. C. da Silva, Marcela Davoli-Ferreira, Christos Tsatsanis, José C. Alves-Filho, Fernando Q. Cunha, Fabrício C. Dias, Helder I. Nakaya, Monique Andrade Baron, Amanda Farage Frade, Tiago Medina, Grace K. Silva, University of Minho [Braga], Universidade do Porto = University of Porto, School of Medicine of Ribeirão Preto (FMRP), Universidade de São Paulo = University of São Paulo (USP), Heart Institute (InCor), Universidade de São Paulo = University of São Paulo (USP)-Faculdade de Medicina FMUSP, Yerkes National Primate Research Center [Lawrenceville, GA], Emory University [Atlanta, GA], Theories and Approaches of Genomic Complexity (TAGC), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Genetics, Biology and Biochemistry, Université de Turin, Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento em Lisboa (INESC-ID), Instituto Superior Técnico, Universidade Técnica de Lisboa (IST)-Instituto de Engenharia de Sistemas e Computadores (INESC), ANR-11-LABX-0024,ParaFrap,Alliance française contre les maladies parasitaires(2011), University of Porto, School of Medicine of Ribeirão Preto ( FMRP ), University of São Paulo ( USP ), Heart Institute ( InCor ), Universidade de São Paulo ( USP ) -Faculdade de Medicina FMUSP, Yerkes National Primate Research Centre, Theories and Approaches of Genomic Complexity ( TAGC ), Aix Marseille Université ( AMU ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Technologies avancées pour le génôme et la clinique ( TAGC ), Instituto de Engenharia de Sistemas e Computadores Investigação e Desenvolvimento em Lisboa ( INESC-ID ), Instituto Superior Técnico, Universidade Técnica de Lisboa ( IST ) -Instituto de Engenharia de Sistemas e Computadores ( INESC ), Universidade do Porto, University of São Paulo (USP), Universidade de São Paulo (USP)-Faculdade de Medicina FMUSP, and Faculdade de Medicina FMUSP-Universidade de São Paulo (USP)
Subjects: Chagas Cardiomyopathy, Male, 0301 basic medicine, Chagas disease, Heart disease, Biopsy, [SDV]Life Sciences [q-bio], Cardiomyopathy, General Physics and Astronomy, 030204 cardiovascular system & hematology, Inbred C57BL, Mice, 0302 clinical medicine, [ SDV.MP ] Life Sciences [q-bio]/Microbiology and Parasitology, Protozoan infections, Class Ib Phosphatidylinositol 3-Kinase, Myeloid Cells, lcsh:Science, ComputingMilieux_MISCELLANEOUS, Phosphoinositide-3 Kinase Inhibitors, Mice, Knockout, Multidisciplinary, Heart, Middle Aged, Canonical, Up-Regulation, 3. Good health, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, Female, Signal transduction, Signal Transduction, Adult, [ SDV.MP.PAR ] Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Myocarditis, Knockout, Trypanosoma cruzi, Science, [ SDV.BBM.BM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Biology, Article, General Biochemistry, Genetics and Molecular Biology, Cell Line, 03 medical and health sciences, Downregulation and upregulation, Quinoxalines, Protozoan infection, parasitic diseases, medicine, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, [ SDV ] Life Sciences [q-bio], Animal, Myocardium, [SDV.BBM.BM]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Molecular biology, Molecular mechanisms, General Chemistry, Disease Models, Animal, Mice, Inbred C57BL, Thiazolidinediones, medicine.disease, biology.organism_classification, 030104 developmental biology, Disease Models, Immunology, MIOCARDIOPATIAS, lcsh:Q
Abstract: Chagas disease is caused by infection with the protozoan Trypanosoma cruzi (T. cruzi) and is an important cause of severe inflammatory heart disease. However, the mechanisms driving Chagas disease cardiomyopathy have not been completely elucidated. Here, we show that the canonical PI3Kγ pathway is upregulated in both human chagasic hearts and hearts of acutely infected mice. PI3Kγ-deficient mice and mutant mice carrying catalytically inactive PI3Kγ are more susceptible to T. cruzi infection. The canonical PI3Kγ signaling in myeloid cells is essential to restrict T. cruzi heart parasitism and ultimately to avoid myocarditis, heart damage, and death of mice. Furthermore, high PIK3CG expression correlates with low parasitism in human Chagas’ hearts. In conclusion, these results indicate an essential role of the canonical PI3Kγ signaling pathway in the control of T. cruzi infection, providing further insight into the molecular mechanisms involved in the pathophysiology of chagasic heart disease., Trypanosoma cruzi infection causes Chagas disease, but mechanisms underlying pathogenesis are unclear. Here, Silva et al. show that canonical PI3Kγ signaling in myeloid cells restricts T. cruzi infection in mice and that high PIK3CG expression correlates with low parasite levels in human Chagas’ hearts.
Published: 2018
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10. New insights into the substrate specificity of macrophage elastase MMP-12

Author: Luiz Juliano, Anni Laffitte, Anne-Sophie Lamort, Rodolphe Gravier, Thierry Moreau, Marie-Louise Zani, Equipe Mecanismes Proteolyt Inflammat (CEPR INSERM U1100), Université de Tours, Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Universidade de São Paulo (USP), Conseil Régional Centre-Val de Loire Programme Investissement d'Avenir Grant Agreement Labex Mab'Improve ANR-10-LABX-53, ANR: ANR-10-LABX-53,programme Investissement d'Avenir Grant Agreement Labex Mab'Improve, Equipe Mecanismes Proteolyt Inflammat ( CEPR INSERM U1100 ), Université Tours, Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Universidade de São Paulo ( USP ), ANR : ANR-10-LABX-53,programme Investissement d'Avenir Grant Agreement Labex Mab'Improve, université de Bourgogne, CSGA, Laboratoires d'excellence - Optimization of therapeutic monoclonal antibodies development Better antibodies, better developed AND better used - - MAbImprove2010 - ANR-10-LABX-0053 - LABX - VALID, Centre d’Etude des Pathologies Respiratoires (CEPR), UMR 1100 (CEPR), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-Centre National de la Recherche Scientifique (CNRS), Universidade de São Paulo = University of São Paulo (USP), ANR-10-LABX-0053,MAbImprove,Optimization of therapeutic monoclonal antibodies development Better antibodies, better developed AND better used(2010), Centre d'Etude des Pathologies Respiratoires (CEPR), UMR 1100. Equipe 2 'Mécanismes Protéolytiques dans l'Inflammation' (CEPR. Equipe 2), Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours (UT)-Institut National de la Santé et de la Recherche Médicale (INSERM), Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Tours-Institut National de la Santé et de la Recherche Médicale (INSERM), and Centre National de la Recherche Scientifique (CNRS)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)
Subjects: 0301 basic medicine, Clinical Biochemistry, Organic chemistry, Peptide, Matrix metalloproteinase, Bioinformatics, Biochemistry, enzyme specificity, macrophage elastase, mmp-12, peptide-protein docking, substrate recognition, Substrate Specificity, Catalytic Domain, Integral membrane protein, chemistry.chemical_classification, biology, Biologie du développement, Hemopexin, Development Biology, peptide, integral membrane-proteins, Molecular Docking Simulation, emphysema, MMP-12, Oligopeptides, mice, Structural similarity, matrix metalloproteinase-12, Macrophage elastase, 03 medical and health sciences, Species Specificity, Matrix Metalloproteinase 12, expression, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Animals, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, [ SDV.BBM ] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Molecular Biology, Fluorescent Dyes, human alpha-1-proteinase inhibitor, copd, alveolar macrophages, Chimie organique, 030104 developmental biology, chemistry, Docking (molecular), Biocatalysis, biology.protein, obstructive pulmonary-disease, Elastin
Abstract: Macrophage elastase, or MMP-12, is mainly produced by alveolar macrophages and is believed to play a major role in the development of chronic obstructive pulmonary disease (COPD). The catalytic domain of MMP-12 is unique among MMPs in that it is very highly active on numerous substrates including elastin. However, measuring MMP-12 activity in biological fluids has been hampered by the lack of highly selective substrates. We therefore synthesized four series of fluorogenic peptide substrates based on the sequences of MMP-12 cleavage sites in its known substrates. Human MMP-12 efficiently cleaved peptide substrates containing a Pro at P3 in the sequence Pro-X-X↓Leu but lacked selectivity towards these substrates compared to other MMPs, including MMP-2, MMP-7, MMP-9 and MMP-13. On the contrary, the substrate Abz-RNALAVERTAS-EDDnp derived from the CXCR5 chemokine was the most selective substrate for MMP-12 ever reported. All substrates were cleaved more efficiently by full-length MMP-12 than by its catalytic domain alone, indicating that the C-terminal hemopexin domain influences substrate binding and/or catalysis. Docking experiments revealed unexpected interactions between the peptide substrate Abz-RNALAVERTAS-EDDn and MMP-12 residues. Most of our substrates were poorly cleaved by murine MMP-12 suggesting that human and murine MMP-12 have different substrate specificities despite their structural similarity.
Published: 2016
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11. A perspective on the need and current status of efficient sex separation methods for mosquito genetic control

Author: Michelle Cristine Pedrosa, Herve C Bossin, Philippos Aris Papathanos, Kostas Bourtzis, Abdoulaye Diabaté, Amadou Guindo, Frédéric Tripet, Jair F. Virginio, Patric Stephane Epopa, Margareth Lara Capurro, Franck Adama Yao, Mamadou B. Coulibaly, Lakamy Sylla, The Hebrew University of Jerusalem (HUJ), Insect Pest Control Laboratory (IPC laboratory), Food and Agriculture Organization of the United Nations [Rome, Italie] (FAO)-International Atomic Energy Agency [Vienna] (IAEA), Keele University [Keele], Vecteurs - Infections tropicales et méditerranéennes (VITROME), Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées [Brétigny-sur-Orge] (IRBA), Biofabrica Moscamed Brazil, Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), Malaria Research and Training Center [Bamako, Mali], Université de Bamako, Centre Muraz [Bobo-Dioulasso, Burkina Faso], FundingPublication costs for this study was provided by the International AtomicEnergy Agency as part of the Coordinated Research Project'Exploringgenetic, molecular, mechanical and behavioural methods of sex separationin mosquitoes', Institut de Recherche Biomédicale des Armées (IRBA)-Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU), and Universidade de São Paulo (USP)-Universidade de São Paulo (USP)
Subjects: Male, 0301 basic medicine, Sex Determination Analysis, Mosquito Control, Review, Sterile insect technique, 0302 clinical medicine, Mosquito, Aedes, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, biology, 3. Good health, Infectious Diseases, Sex separation, Scale (social sciences), Female, Research program, ANIMAIS PARASITOS, 030231 tropical medicine, Control (management), Gene drive, Genetic control, Genetic sexing strains, Sexing, Mosquito Vectors, lcsh:Infectious and parasitic diseases, 03 medical and health sciences, Anopheles, medicine, Animals, Humans, lcsh:RC109-216, Environmental planning, [SDV.EE.SANT]Life Sciences [q-bio]/Ecology, environment/Health, business.industry, QH, Gene Drive Technology, biology.organism_classification, medicine.disease, Malaria, 030104 developmental biology, Agriculture, Infertility, Parasitology, business
Abstract: International audience; Major efforts are currently underway to develop novel, complementary methods to combat mosquito-borne diseases. Mosquito genetic control strategies (GCSs) have become an increasingly important area of research on account of their species-specificity, track record in targeting agricultural insect pests, and their environmentally non-polluting nature. A number of programs targeting Aedes and Anopheles mosquitoes, vectors of human arboviruses and malaria respectively, are currently being developed or deployed in many parts of the world. Operationally implementing these technologies on a large scale however, beyond proof-of-concept pilot programs, is hampered by the absence of adequate sex separation methods. Sex separation eliminates females in the laboratory from male mosquitoes prior to release. Despite the need for sex separation for the control of mosquitoes, there have been limited efforts in recent years in developing systems that are fit-for-purpose. In this special issue of Parasites and Vectors we report on the progress of the global Coordinated Research Program on Exploring genetic, molecular, mechanical and behavioural methods for sex separation in mosquitoes that is led by the Insect Pest Control Subprogramme of the Joint FAO/IAEA Division of Nuclear Techniques in Food and Agriculture with the specific aim of building efficient sex separation systems for mosquito species. In an effort to overcome current barriers we briefly highlight what we believe are the three main reasons why progress has been so slow in developing appropriate sex separation systems: the availability of methods that are not scalable, the difficulty of building the ideal genetic systems and, finally, the lack of research efforts in this area.
Published: 2018
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12. Chagas Disease Cardiomyopathy: Immunopathology and Genetics

Author: Christophe Chevillard, Edecio Cunha-Neto, Heart Institute (InCor), Universidade de São Paulo = University of São Paulo (USP)-Faculdade de Medicina FMUSP, Institute for Investigation in Immunology (III), National Institute of Science and Technology (INCT), Division of Clinical Immunology and Allergy, University of São Paulo School of Medicine, Génétique et immunologie des maladies parasitaires (GIMP), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Universidade de São Paulo (USP)-Faculdade de Medicina FMUSP, Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), and Faculdade de Medicina FMUSP-Universidade de São Paulo (USP)
Subjects: Chagas Cardiomyopathy, Chagas disease, Myocarditis, Immunology, Cardiomyopathy, Review Article, Asymptomatic, Immunopathology, lcsh:Pathology, medicine, Animals, Humans, Trypanosoma cruzi, ComputingMilieux_MISCELLANEOUS, Genetics, biology, business.industry, Mortality rate, Family aggregation, Cell Biology, medicine.disease, biology.organism_classification, 3. Good health, MIOCARDIOPATIA CHAGÁSICA, [SDV.MP]Life Sciences [q-bio]/Microbiology and Parasitology, medicine.symptom, business, lcsh:RB1-214
Abstract: Chagas disease, caused by the protozoanTrypanosoma cruzi, is endemic in Latin America and affects ca. 10 million people worldwide. About 30% of Chagas disease patients develop chronic Chagas disease cardiomyopathy (CCC), a particularly lethal inflammatory cardiomyopathy that occurs decades after the initial infection, while most patients remain asymptomatic. Mortality rate is higher than that of noninflammatory cardiomyopathy. CCC heart lesions present a Th1 T-cell-rich myocarditis, with cardiomyocyte hypertrophy and prominent fibrosis. Data suggest that the myocarditis plays a major pathogenetic role in disease progression. Major unmet goals include the thorough understanding of disease pathogenesis and therapeutic targets and identification of prognostic genetic factors. Chagas disease thus remains a neglected disease, with no vaccines or antiparasitic drugs proven efficient in chronically infected adults, when most patients are diagnosed. Both familial aggregation of CCC cases and the fact that only 30% of infected patients develop CCC suggest there might be a genetic component to disease susceptibility. Moreover, previous case-control studies have identified some genes associated to human susceptibility to CCC. In this paper, we will review the immunopathogenesis and genetics of Chagas disease, highlighting studies that shed light on the differential progression of Chagas disease patients to CCC.
Published: 2014
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13. Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences

Author: Fenton P.D. Cotterill, Fernanda P. Werneck, Stephen W. Chordas, Enrique González-Soriano, Pierangelo Luporini, Santiago Claramunt, Santosh Kumar, Adriano B. Kury, Marcelo José Sturaro, Atsushi Tominaga, Marcos Gonçalves Lhano, Giulio Cuccodoro, Bernardo F. Santos, Alejandro Oceguera-Figueroa, Klaus Henle, Giovanni B. Delmastro, Thibaut Delsinne, Jeremy A. Miller, Thomas Ziegler, Ishan Agarwal, Rodrigo M. Feitosa, Robert C. Glotzhober, Giuliano Doria, Adeline Soulier-Perkins, Diego Baldo, Valéria da Cunha Tavares, Danilo Pacheco Cordeiro, Eli Greenbaum, Carlos Alberto Santos de Lucena, Stuart V. Nielsen, Jörn Köhler, Fernando Pacheco Rodrigues, Justin C. Bagley, Shun Ichiro Naomi, Gustavo Hormiga, Geoffrey Odhiambo Ong'ondo, Aurélien Miralles, Alexandre Uarth Christoff, Florian M. Steiner, Matthias Glaubrecht, Victor Van Cakenberghe, Wolfgang Rabitsch, Jack W. Sites, Norma J. Salcedo, Mario Alberto Cozzuol, Ward C. Wheeler, Krister T. Smith, Brian Tilston Smith, Ignacio Jose De La Riva De La Viña, Leo J. Borkin, Ângelo Parise Pinto, Marivene R. Manuel-Santos, Ana Carolina Pavan, M. J. Alves, Dan Cogălniceanu, Luciana F. Santoferrara, James M. Carpenter, Thierry Deuve De Resbecq, Beat Schätti, Jean Pierre Vacher, John G. Day, Ray C. Schmidt, Otto M. P. Oliveira, Lázaro Guevara, Jean-Lou Justine, Karthikeyan Vasudevan, Donat Agosti, Cécile Mourer-Chauviré, Brett C. Ratcliffe, Birgit C. Schlick-Steiner, Sebastian Kvist, Nathan K. Lujan, Robert Alexander Pyron, Rosana M. Rocha, Roberto Poggi, José A. Langone, Larry Lee Grismer, Václav Gvoždík, Natsuhiko Yoshikawa, Thaís P. Miranda, Elizabeth Prendini, Abel Pérez-González, Katharina C. Wollenberg Valero, Jean-Yves Rasplus, Cristiano R. Moreira, Antonietta La Terza, Fabio Siqueira Pitaluga de Godoi, Michael W. Holmes, Thomas E. Lacher, Ronald H. Pine, Matthew P. Heinicke, Steven M. Goodman, John D. Lynch, Elöd Kondorosy, Anderson Feijó, Orfeo Picariello, Wolfgang Denzer, Stefano Valdesalici, Aléssio Datovo, Jean Pierre Hugot, Yuri L. R. Leite, Heinz Grillitsch, Hernán Ortega, Dimitri Forero, Jean Carlos Santos, Marie Claude Durette-Desset, Victor H. Gonzalez, Mrugank Prabhu, Walter E. Schargel, Beate Röll, Caleb D. McMahan, Mitsuru Kuramoto, Edson A. Adriano, Jérôme Constant, Richard Laval, María A. Mendoza-Becerril, Cédric d'Udekem d'Acoz, Alain Didier Missoup, Frank Tillack, Janet K. Braun, Lindsey Swierk, André L. Netto-Ferreira, Xiaofeng Lin, Karl Heinz Jungfer, Fabio Di Dario, Vanessa Kruth Verdade, Pavel Štys, Franco Andreone, Andrés A. Ojanguren-Affilastro, Manuel Ruedi, Didier Van den Spiegel, Rahul Khot, Lars Krogmann, Lance Grande, Robert C. Drewes, Luis M. P. Ceríaco, Jeffrey W. Streicher, Jacob A. Esselstyn, Josiah H. Townsend, Wolfgang Arthofer, Wiesław Bogdanowicz, Marcos A. Raposo, Omar Torres-Carvajal, Dirk Ahrens, Theo Blick, Carlos DoNascimiento, Eric Drouet, Claudia Patricia Ornelas-García, Gervásio Silva Carvalho, Zachary H. Falin, Gaetano Odierna, Michael Maia Mincarone, Sabine Agatha, Christian De Muizon, Célio F. B. Haddad, Pablo Rodrigues Gonçalves, Maarten P.M. Vanhove, Ronald Janssen, Ulrich Burkhardt, Bernard Landry, Paúl M. Velazco, Melanie L. J. Stiassny, Erna Aescht, Sarah Siqueira Oliveira, Koshiro Eto, Thomas van de Kamp, Fabio Cianferoni, Leonardo Ferreira Machado, Luiz Carlos Pinho, Dennis Rödder, Fábio Raposo do Amaral, Shan Gao, Paulo Passos, Nikolai L. Orlov, Emanuel Tschopp, Bert Van Bocxlaer, Roman Hołyński, Isabella Van De Velde, Indraneil Das, Luciano Damián Patitucci, Daniel J. Bennett, Annemarie Ohler, Rachunliu G. Kamei, Patrick Grootaert, Tony Robillard, Jun Gong, Massimo Delfino, Antonio C. Marques, Daizy Bharti, Ira Richling, José L. O. Birindelli, Thiago Borges Fernandes Semedo, Philippe Grandcolas, Eric J. Sargis, Andreas Taeger, Jesús Molinari, Link E. Olson, Christoph Kucharzewski, Luc Janssens de Bisthoven, José P. Pombal, Ryan C. McKellar, Serge Gofas, Mário C. C. de Pinna, Kristofer M. Helgen, Pablo Quintela-Alonso, Marcos Tavares, Wolfgang A. Nässig, Jodi J. L. Rowley, Jairo Arroyave, Fabio Maria Guarino, Djoko T. Iskandar, Martin Fikáček, Joel Cracraft, Robert M. Timm, Lassad Neifar, Marcelo C. Andrade, Moisés Escalona, Max Kieckbusch, George R. Zug, J. V. Remsen, Weibo Song, Paula Beatriz Araujo, Marco Brandalise de Andrade, Luiz Alexandre Campos, Eva V. Bärmann, Thomas Lehmann, Thorsten Stoeck, Jorge Salazar-Bravo, Charles Morphy D. Santos, Joël Minet, Mann Kyoon Shin, Gustavo A. Bravo, Felipe Franco Curcio, Antoine Pariselle, Hidetoshi Ota, David R. Luz, Abdulaziz S. Alqarni, Joseph A. Cook, Cameron D. Siler, Zilda Margarete Seixas de Lucena, Guarino R. Colli, Máriom A. Carvajal, Franziska Bauer, Yves Samyn, Luke Tornabene, Stefan Merker, Favízia Freitas de Oliveira, Murilo N. L. Pastana, Luís Fábio Silveira, Moira Jane FitzPatrick, Stephen D. Busack, Max R. Lambert, Julián Faivovich, Masafumi Matsui, Bernhard A. Huber, Alexandre Aleixo, Mariana P. Marques, Jean-François Trape, Marcello Guimarães Simões, Brian L. Fisher, Brandi S. Coyner, Michael F. Bates, Marcelo Salles Rocha, Silke Schweiger, Jean Raffaëlli, Vladimir Dinets, Paulo C. A. Garcia, Devanshu Gupta, Juan M. Guayasamin, W. Brian Simison, Rudy Jocqué, Aniruddha Datta-Roy, Marcelo R. Britto, Cristiane Bastos-Silveira, Celso O. Azevedo, Roger Bour, Aidin Niamir, Leandro M. Vieira, Mark Epstein, Neal Woodman, Marcelo R. de Carvalho, José Antonio González-Orej, Martin Kruger, Ulisses Caramaschi, Marcus Guidoti, Cibele Biondo, Scott Lyell Gardner, François Dusoulier, Francisco Langeani, John E. Lattke, Helen M. Barber-James, Jan Zima, Guilherme R. R. Brito, Ricardo Moratelli, Stylianos Chatzimanolis, Carlos José Einicker Lamas, John B. Iverson, Maria Hołyńska, Aaron M. Bauer, Luc Brendonck, Klaus-Peter Koepfli, Angelica Crottini, Cristian Hernan Fulvio Perez, Tiago Georg Pikart, Eliécer E. Gutiérrez, Luis García-Prieto, Lawrence R. Heaney, Thomas A. Munroe, Thomas C. Giarla, Laurie J. Vitt, Enrico Borgo, Antonio J. C. Aguiar, Sven O. Kullander, Jean Sébastien Steyer, Marcial Quiroga-Carmona, Matthew J. Miller, Kraig Adler, Werner Conradie, Enrique La Marca, Thomas Schmitt, Dieter Uhl, Mario de Vivo, Rainer Hutterer, Silvio Shigueo Nihei, Perry L. Wood, Amira Chaabane, Tim Tokaryk, Octávio Mateus, Andrés Sebastián Quinteros, Daniel S. Fernandes, Alexandra Cartaxana, Pedro F. Victoriano, Ernest C.J. Seamark, William R. Branch, Mark-Oliver Rödel, Diego Astúa, Marcio R. Pie, Julien Pétillon, Henrard Arnaud, Hossein Rajaei, Sushil K. Dutta, Hussam Zaher, Hernández Díaz Yoalli Quetzalli, Martin Carr, Renan Carrenho, Estefanía Rodríguez, Robert Trusch, Patrick David, Rafaela Lopes Falaschi, Rafael O. de Sá, Miguel Ângelo Marini, Varad B. Giri, Jean-Claude Rage, Guilherme S. T. Garbino, Björn Berning, Thierry Frétey, Vítor de Q. Piacentini, Paulo A. Buckup, David C. Lees, Alfred L. Gardner, Marco Pavia, Pablo Ricardo Mulieri, Lorenzo Prendini, Eliana M. Cancello, Cinthia Chagas, Bruce B. Collette, Leigh R. Richards, Eduardo I. Faúndez, Timothy J. Colston, Thomas Keith Philips, Miguel Trefaut Rodrigues, Renato Gregorin, Karin Meißner, Nathan S. Upham, A. Townsend Peterson, Tiago Kütter Krolow, Felipe Ferraz Figueiredo Moreira, Olivier Montreuil, Leandro M. Sousa, Thomas Weisse, Natalia B. Ananjeva, Donald C. Taphorn, Renata Stopiglia, Marcelo Duarte, Benoit Guénard, Cyril Gallut, Giovanni Boano, David Modrý, Erik Verheyen, Jonas José Mendes Aguiar, Sven Mecke, Alexandre Hassanin, Robert M. Zink, Marcello Mezzasalma, André Silva Roza, Reginaldo Constantino, Alice Hirschmann, Ulisses Pinheiro, Edmundo González-Santillán, Carlos A. Mendoza-Palmero, Tom Artois, Fernando J. M. Rojas-Runjaic, Kailas Chandra, Pablo Teta, Michael Karner, Esteban O. Lavilla, Mauricio Ortega-Andrade, Alexandra Marçal Correia, Deepak Veerappan, Daniela M. Takiya, Bolívar R. Garcete-Barrett, Alexander Kupfer, Sérgio N. Stampar, Daniel Burckhardt, Michael S. Engel, Teresa Kearney, Silvia E. Pavan, Luiz Roberto Malabarba, Mark D. Scherz, Pedro L. V. Peloso, Christiane Denys, Matthias F. Geiger, Alexander Pelzer, Jose G. Tello, Fabio S. Nascimento, Juan D. Daza, Franger J. García, Cinthia A. Brasileiro, Martín J. Ramírez, Marcos Pérsio Dantas Santos, Twan A. A. M. Leenders, Alain Canard, Tomáš Mazuch, Axel Hausmann, Flávio Alicino Bockmann, Prosanta Chakrabarty, Jasmine Purushothaman, Ara Monadjem, David A. Donoso, Kaushik Deuti, Stephen Mahony, Duke S. Rogers, Don E. Wilson, Julian C. Kerbis Peterhans, Jader Marinho-Filho, Alain Dubois, Marcio Luiz de Oliveira, Jan Decher, John M. Midgley, Fernando C. Jerep, Bastian Bentlage, Ivan Löbl, Gregory J. Watkins-Colwell, Uwe Fritz, Annamaria Nistri, Lúcia H. Rapp Py-Daniel, Bruce D. Patterson, Peter J. Taylor, Burton K. Lim, James L. Patton, Colin S. Schoeman, Stéphane Grosjean, Ismael Franz, Cristian Simón Abdala, John S. Sparks, Marcos R. Bornschein, Leonora Pires Costa, Martín O. Pereyra, João Filipe Riva Tonini, Richard Schodde, Blanca Pérez-Luz, Cristiano Feldens Schwertner, Peter Jäger, Marcin Jan Kamiński, Philipp Wagner, Jakob Hallermann, Hendrik Freitag, Olavi Kurina, Laure Desutter-Grandcolas, Romain Garrouste, Pedro De Podestà Uchôa de Aquino, Guillermo D’Elía, Sharlene E. Santana, Roberto E. Reis, Wouter Dekoninck, Sushma Reddy, Alfred L. Rosenberger, James R. McCranie, Wolfgang Böhme, Ricardo C. Benine, Cyrille D'Haese, Paulo H. F. Lucinda, Jacques H. C. Delabie, Carr, Martin, Department of Biology, Northern Arizona University [Flagstaff], Museu Nacional de Historia Natural e da Ciencia, Departamento de Zoologia, Instituto de Ciências Biológicas, Universidade de Brasilia [Brasília] (UnB), National Museum of Natural History, National Museum of Natural History - Leiden, Institut de Systématique, Evolution, Biodiversité (ISYEB ), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École pratique des hautes études (EPHE)-Centre National de la Recherche Scientifique (CNRS), École pratique des hautes études (EPHE), Université Pierre et Marie Curie (Paris 6), Centre National de la Recherche Scientifique (CNRS), Sorbonne Universités, Universidad Nacional de Tucumán (UNT), King Saud University, Cornell University, Universidade Federal de São Paulo, Austrian Museum, Villanova University, Universität Salzburg, Plazi, University of São Paulo, Zoologisches Forschungsmuseum Alexander Koenig, Museu Paraense Emílio Goeldi, Russian Academy of Sciences, Federal University of Para - Universidade Federal do Para [Belem - Brésil], Pontificia Universidade Catolica do Rio Grande do Sul (PUCRS), Museo Regionale di Scienze Naturali, Universidade Federal do Rio Grande do Sul [Porto Alegre] (UFRGS), Royal Museum for Central Africa [Tervuren] (RMCA), Universidad Nacional Autónoma de México (UNAM), Institute of Ecology, Technische Universität Berlin (TUB), Hasselt University, Universidade Federal de Pernambuco [Recife] (UFPE), Universidade Federal do Espírito Santo (UFES), Institut de Recherche pour le Développement (IRD [Nouvelle-Calédonie]), Albany Museum, National Museum, Senckenberg Naturhistorische Sammlungen, Universidade Estadual Paulista Júlio de Mesquita Filho [São José do Rio Preto] (UNESP), Stephen F. Austin State University, Smithsonian Institution, Tyrolean State Museum, Università di Camerino, Universidade Federal do ABC, Museu de Zoologia da Universidade Estadual de Londrina, Senckenberg Research Institute, Museo Civico di Storia Naturale, Muzeum i Instytut Zoologii Polskiej Akademii Nauk, Russian Academy of Sciences [Moscow] (RAS), Port Elizabeth Museum, Sam Noble Museum, Harvard University [Cambridge], North West University, Museu Nacional do Rio de Janeiro, Musée d'Histoire Naturelle de Bâle, Senckenberg Museum [Frankfurt], North Carolina Museum of Natural Sciences, Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES), Museu de Zoologia (MZ), Universidade de São Paulo (USP), American Museum of Natural History, University of Huddersfield, North Dakota State University (NDSU), Faculté des Sciences de Sfax, Université de Sfax - University of Sfax, Departamento de Polícia Técnico Científica (DPTC), Museum of Natural Science, Louisiana State University (LSU), Zoological Survey of India, University of Tennessee System, Ohio State University [Columbus] (OSU), Museu de Ciências Naturais, Universidade Luterana do Brasil (ULBRA), Museo di Storia Naturale, Università degli Studi di Firenze, Ovidius University of Constanta, The University of Mississippi [Oxford], Royal Belgian Institute of Natural Sciences (RBINS), The University of New Mexico [Albuquerque], Instituto Nacional de Pesquisas da Amazônia (INPA), University of Stellenbosh, Universidade Federal de Minas Gerais, Centro de Investigaçao em Biodiversidade e Recursos Genéticos, Museum d'Histoire Naturelle, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Muséum national d'Histoire naturelle (MNHN)-École pratique des hautes études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), Muséum national d'Histoire naturelle (MNHN)-Université Pierre et Marie Curie - Paris 6 (UPMC)-École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Centre National de la Recherche Scientifique (CNRS), École Pratique des Hautes Études (EPHE), Université Paris sciences et lettres (PSL), Université Pierre et Marie Curie - Paris 6 (UPMC), Sorbonne Université (SU), King Saud University [Riyadh] (KSU), Cornell University [New York], Villanova University [USA], Universidade de São Paulo = University of São Paulo (USP), Museu Paraense Emílio Goeldi [Belém, Brésil] (MPEG), the Russian Academy of Sciences [Moscow, Russia] (RAS), Federal University of Para - Universidade Federal do Pará - UFPA [Belém, Brazil] (UFPA), Universidad Nacional Autónoma de México = National Autonomous University of Mexico (UNAM), Technical University of Berlin / Technische Universität Berlin (TU), Hasselt University (UHasselt), Universidade Federal do Espirito Santo (UFES), Universidade Estadual Paulista Júlio de Mesquita Filho = São Paulo State University (UNESP), Università degli Studi di Camerino = University of Camerino (UNICAM), Harvard University, North-West University [Potchefstroom] (NWU), Université de Rennes (UR), American Museum of Natural History (AMNH), Museo di Storia Naturale di Firenze, Università degli Studi di Firenze = University of Florence (UniFI), Stellenbosch University, Museum d'Histoire Naturelle [Genève] (MHN), Ceríaco, Luis M. P., Gutiérrez, Eliécer E., Dubois, Alain, Abdala, Cristian Simón, Alqarni, Abdulaziz S., Adler, Kraig, Adriano, Edson A., Aescht, Erna, Agarwal, Ishan, Agatha, Sabine, Agosti, Donat, Aguiar, Antonio J. C., Aguiar, Jonas José Mende, Ahrens, Dirk, Aleixo, Alexandre, Alves, Maria Judite, Do Amaral, Fabio Raposo, Ananjeva, Natalia, Andrade, Marcelo C., De Andrade, Marco Brandalise, Andreone, Franco, Aquino, Pedro P. U., Araujo, Paula Beatriz, Arnaud, Henrard, Arroyave, Jairo, Arthofer, Wolfgang, Artois, Tom J., Astúa, Diego, Azevedo, Celso, Bagley, Justin C., Baldo, Diego, Barber James, Helen Margaret, Bärmann, Eva V., Bastos Silveira, Cristiane, Bates, Michael F., Bauer, Aaron M., Bauer, Franziska, Benine, Ricardo C., Bennett, Daniel J., Bentlage, Bastian, Berning, Björn, Bharti, Daizy, Biondo, Cibele, Birindelli, José, Blick, Theo, Boano, Giovanni, Bockmann, Flávio A., Bogdanowicz, Wieslaw, Böhme, Wolfgang, Borgo, Enrico, Borkin, Leo, Bornschein, Marcos Ricardo, Bour, Roger, Branch, William R., Brasileiro, Cinthia A., Braun, Janet K., Bravo, Gustavo A., Brendonck, Luc, Brito, Guilherme R. R., Britto, Marcelo R., Buckup, Paulo A., Burckhardt, Daniel, Burkhardt, Ulrich, Busack, Stephen D., Campos, Luiz A., Canard, Alain, Cancello, Eliana M., Caramaschi, Ulisse, Carpenter, James M., Carrenho, Renan, Cartaxana, Alexandra, Carvajal, Mariom A., Carvalho, Gervásio Silva, De Carvalho, Marcelo Rodrigue, Chaabane, Amira, Chagas, Cinthia, Chakrabarty, Prosanta, Chandra, Kaila, Chatzimanolis, Styliano, Chordas, Stephen W., Christoff, Alexandre U., Cianferoni, Fabio, Claramunt, Santiago, Cogãlniceanu, Dan, Collette, Bruce B., Colli, Guarino R., Colston, Timothy J., Conradie, Werner, Constant, Jérôme, Constantino, Reginaldo, Cook, Joseph A., Cordeiro, Danilo, Correia, Alexandra Marçal, Cotterill, Fenton P. D., Coyner, Brandi, Cozzuol, Mario A., Cracraft, Joel, Crottini, Angelica, Cuccodoro, Giulio, Curcio, Felipe Franco, D'Udekem D'Acoz, Cédric, D'Elía, Guillermo, D'Haese, Cyrille, Das, Indraneil, Datovo, Aléssio, Datta Roy, Aniruddha, David, Patrick, Day, John G., Daza, Juan D., De Bisthoven, Luc Janssen, De La Riva De La Viña, Ignacio Jose, De Muizon, Christian, De Pinna, Mario, Piacentini, Vítor De Q., De Sá, Rafael O., De Vivo, Mario, Decher, Jan, Dekoninck, Wouter, Delabie, Jacques H. C., Delfino, Massimo, Delmastro, Giovanni B., Delsinne, Thibaut, Denys, Christiane, Denzer, Wolfgang, Desutter Grandcolas, Laure, Deuti, Kaushik, De Resbecq, Thierry Deuve, Di Dario, Fabio, Dinets, Vladimir, Donascimiento, Carlo, Donoso, David A., Doria, Giuliano, Drewes, Robert C., Drouet, Eric, Duarte, Marcelo, Durette Desset, Marie Claude, Dusoulier, Françoi, Dutta, Sushil Kumar, Engel, Michael S., Epstein, Mark, Escalona, Moisé, Esselstyn, Jacob A., Eto, Koshiro, Faivovich, Julián, Falaschi, Rafaela Lope, Falin, Zachary H., Faundez, Eduardo I., Feijó, Anderson, Feitosa, Rodrigo M., Fernandes, Daniel Silva, Fikáček, Martin, Fisher, Brian L., Fitzpatrick, Moira J., Forero, Dimitri, Franz, Ismael, Freitag, Hendrik, Frétey, Thierry, Fritz, Uwe, Gallut, Cyril, Gao, Shan, Garbino, Guilherme S. T., Garcete Barrett, Bolívar R., García Prieto, Lui, García, Franger J., Garcia, Paulo C. A., Gardner, Alfred L., Gardner, Scott Lyell, Garrouste, Romain, Geiger, Matthias F., Giarla, Thomas C., Giri, Varad, Glaubrecht, Matthia, Glotzhober, Robert C., Godoi, Fabio S. P., Gofas, Serge, Gonçalves, Pablo R., Gong, Jun, Gonzalez, Victor H., González Orej, José Antonio, González Santillán, Edmundo, González Soriano, Enrique, Goodman, Steven M., Grandcolas, Philippe, Grande, Lance, Greenbaum, Eli, Gregorin, Renato, Grillitsch, Heinz, Grismer, Larry Lee, Grootaert, Patrick, Grosjean, Stéphane, Guarino, FABIO MARIA, Guayasamin, Juan M., Guénard, Benoit, Guevara, Lázaro, Guidoti, Marcu, Gupta, Devanshu, Gvoždík, Václav, Haddad, Célio F. B., Hallermann, Jakob, Hassanin, Alexandre, Hausmann, Axel, Heaney, Lawrence R., Heinicke, Matthew P., Helgen, Kristofer M., Henle, Klau, Hirschmann, Alice, Holmes, Michael W., Hołyńska, Maria, Hołyński, Roman, Hormiga, Gustavo, Huber, Bernhard A., Hugot, Jean Pierre, Hutterer, Rainer, Iskandar, Djoko, Iverson, John B., Jäger, Peter, Janssen, Ronald, Jerep, Fernando, Jocqué, Rudy, Jungfer, Karl Heinz, Justine, Jean Lou, Kamei, Rachunliu G., Kamiński, Marcin Jan, Karner, Michael, Kearney, Teresa, Khot, Rahul, Kieckbusch, Max, Köhler, Jörn, Koepfli, Klaus Peter, Kondorosy, Elöd, Krogmann, Lar, Krolow, Tiago Kütter, Krüger, Martin, Kucharzewski, Christoph, Kullander, Sven O., Kumar, Santosh, Kupfer, Alexander, Kuramoto, Mitsuru, Kurina, Olavi, Kury, Adriano, Kvist, Sebastian, La Marca, Enrique, La Terza, Antonietta, Laval, Richard, Lacher, Thomas E., Lamas, Carlos J. E., Lambert, Max R., Landry, Bernard, Langeani, Francisco, Langone, José A., Lattke, John E., Lavilla, Esteban O., Leenders, Twan, Lees, David C., Leite, Yuri L. R., Lehmann, Thoma, Lhano, Marcos Gonçalve, Lim, Burton K., Lin, Xiaofeng, Löbl, Ivan, De Lucena, Carlos A. S., De Lucena, Zilda Margarete S., Lucinda, Paulo, Lujan, Nathan K., Luporini, Pierangelo, Luz, David R., Lynch, John D., Machado, Leonardo Ferreira, Mahony, Stephen, Malabarba, Luiz R., Manuel Santos, Marivene, Marinho Filho, Jader, Marini, Miguel Â., Marques, Antonio Carlo, Marques, Mariana P., Mateus, Octávio, Matsui, Masafumi, Mazuch, Tomáš, Mccranie, Jame, Mckellar, Ryan C., Mcmahan, Caleb D., Mecke, Sven, Meißner, Karin, Mendoza Becerril, María A., Mendoza Palmero, Carlos A., Merker, Stefan, Mezzasalma, Marcello, Midgley, John Mark, Miller, Jeremy, Miller, Matthew J., Mincarone, Michael Maia, Minet, Joël, Miralles, Aurélien, Miranda, Thaís P., Missoup, Alain Didier, Modrý, David, Molinari, Jesú, Monadjem, Ara, Montreuil, Olivier, Moratelli, Ricardo, Moreira, Cristiano Rangel, Moreira, Felipe F. F., Mourer Chauviré, Cécile, Mulieri, Pablo Ricardo, Munroe, Thomas A., Naomi, Shun Ichiro, Nascimento, Fabio, Nässig, Wolfgang A., Neifar, Lassad, Netto Ferreira, Andre L., Niamir, Aidin, Nielsen, Stuart V., Nihei, Silvio S., Nistri, Annamaria, Oceguera Figueroa, Alejandro, Odierna, Gaetano, Ohler, Annemarie, Ojanguren Affilastro, Andres A., De Oliveira, Favízia Freita, De Oliveira, Marcio Luiz, De Oliveira, Otto Müller Patrão, Oliveira, Sarah Siqueira, Olson, Link E., Ong'Ondo, Geoffrey O., Orlov, Nikolai, Ornelas García, Claudia Patricia, Ortega, Hernan, Ortega Andrade, Mauricio, Ota, Hidetoshi, Pariselle, Antoine, Passos, Paulo, Pastana, Murilo N. L., Patterson, Bruce D., Patitucci, Luciano D., Patton, James L., Pavan, Ana C., Pavan, Silvia E., Pavia, Marco, Peloso, Pedro L. V., Pelzer, Alexander, Pereyra, Martín O., Perez Gonzalez, Abel, Pérez Luz, Blanca, Pérez, Cristian Hernan Fulvio, Peterhans, Julian Kerbi, Peterson, A. Townsend, Pétillon, Julien, Philips, Thomas Keith, Picariello, ORFEO LUCIO ANTONIO, Pie, Marcio R., Pikart, Tiago G., Pine, Ronald H., Pinheiro, Ulisse, Pinho, Luiz Carlo, Pinto, Ângelo P., Costa, Leonora Pire, Poggi, Roberto, Pombal, José P., Prabhu, Mrugank, Prendini, Elizabeth, Prendini, Lorenzo, Purushothaman, Jasmine, Pyron, Robert Alexander, Quintela Alonso, Pablo, Quinteros, Andres Sebastian, Quiroga Carmona, Marcial, Rabitsch, Wolfgang, Raffaëlli, Jean, Rage, Jean Claude, Rajaei, Hossein, Ramírez, Martín J., Raposo, Marcos A., Py Daniel, Lucia H. Rapp, Rasplus, Jean Yve, Ratcliffe, Brett C., Reddy, Sushma, Reis, Roberto E., Remsen, James V., Richards, Leigh R., Richling, Ira, Robillard, Tony, Rocha, Marcelo Salle, Rocha, Rosana Moreira, Rödder, Denni, Rödel, Mark Oliver, Rodrigues, Fernando P., Rodriguez, Estefania, Rogers, Duke S., Rojas Runjaic, Fernando J. M., Röll, Beate, Rosenberger, Alfred L., Rowley, Jodi, Roza, André Silva, Ruedi, Manuel, Salazar Bravo, Jorge, Salcedo, Norma J., Samyn, Yve, Santana, Sharlene E., Santoferrara, Luciana, Santos, Bernardo F., Santos, Charles Morphy D., Santos, Jean Carlo, Santos, Marcos Pérsio Danta, Sargis, Eric J., Schargel, Walter E., Schätti, Beat, Scherz, Mark D., Schlick Steiner, Birgit C., Schmidt, Ray C., Schmitt, Thoma, Schodde, Richard, Schoeman, Colin S., Schweiger, Silke, Schwertner, Cristiano F., Seamark, Ernest C. J., Semedo, Thiago B. F., Shin, Mann Kyoon, Siler, Cameron D., Silveira, Luís Fábio, Simison, W. Brian, Simões, Marcello, Sites, Jack W., Smith, Brian Tilston, Smith, Krister T., Song, Weibo, Soulier Perkins, Adeline, Sousa, Leandro M., Sparks, John S., Stampar, Sérgio N., Steiner, Florian M., Steyer, Jean Sébastien, Stiassny, Melanie L. J., Stoeck, Thorsten, Stopiglia, Renata, Streicher, Jeffrey W., Sturaro, Marcelo J., Stys, Pavel, Swierk, Lindsey, Taeger, Andrea, Takiya, Daniela M., Taphorn, Donald C., Tavares, Marco, Tavares, Valeria Da C., Taylor, Peter John, Tello, Jose G., Teta, Pablo, Tillack, Frank, Timm, Robert M., Tokaryk, Tim, Tominaga, Atsushi, Tonini, João Filipe Riva, Tornabene, Luke, Torres Carvajal, Omar, Townsend, Josiah, Trape, Jean Françoi, Rodrigues, Miguel Trefaut, Trusch, Robert, Tschopp, Emanuel, Uhl, Dieter, Upham, Nathan S., Vacher, Jean Pierre, Valdesalici, Stefano, Van Bocxlaer, Bert, Van Cakenberghe, Victor, Van De Kamp, Thoma, Van De Velde, Isabella, Van Den Spiegel, Didier, Vanhove, Maarten P. M., Vasudevan, Karthikeyan, Veerappan, Deepak, Velazco, Paúl M., Verdade, Vanessa K., Verheyen, Erik, Vieira, Leandro M., Victoriano, Pedro F., Vitt, Laurie J., Wagner, Philipp, Watkins Colwell, Gregory J., Weisse, Thoma, Werneck, Fernanda P., Wheeler, Ward C., Wilson, Don E., Valero, Katharina C. Wollenberg, Wood, Perry Lee, Woodman, Neal, Quetzalli, Hernández Díaz Yoalli, Yoshikawa, Natsuhiko, Zaher, Hussam, Ziegler, Thoma, Zima, Jan, Zink, Robert M., Zug, George, Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Centre National de la Recherche Scientifique (CNRS), Technische Universität Berlin (TU), Università degli Studi di Camerino (UNICAM), Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Universidade de Brasília, Institut de Systématique, Evolution, Biodiversité ( ISYEB ), Muséum National d'Histoire Naturelle ( MNHN ) -Université Pierre et Marie Curie - Paris 6 ( UPMC ) -Centre National de la Recherche Scientifique ( CNRS ) -École pratique des hautes études ( EPHE ), Ecole Pratique des Hautes Etudes ( EPHE ), Centre National de la Recherche Scientifique ( CNRS ), Universidad Nacional de Tucumán, Villanova University [Philadelphie], University of Salzburg, Museu Paraense Emilio Goeldi, Universidade Federal do Pará, Pontificia Universidade Catolica do Rio Grande do Sul ( PUCRS ), Universidade Federal do Rio Grande do Sul ( UFRGS ), Royal Museum for Central Africa, Universidad Nacional Autónoma de México ( UNAM ), Technical University of Berlin, Universidade Federal de Pernambuco ( UFPE ), Universidade Federal do Espírito Santo ( UFES ), Institut de Recherche pour le Développement ( IRD [Nouvelle-Calédonie] ), Universidade Estadual Paulista Julio de Mesquita Filho ( UNESP ), Russian Academy of Sciences [Moscow] ( RAS ), Senckenberg Museum, Université de Rennes 1 ( UR1 ), Université de Rennes ( UNIV-RENNES ), Museu de Zoologia ( MZ ), Universidade de São Paulo ( USP ), North Dakota State University ( NDSU ), Departamento de Polícia Técnico Científica ( DPTC ), Louisiana State University ( LSU ), University of Tennessee, Ohio State University [Columbus] ( OSU ), Universidade Luterana do Brasil ( ULBRA ), University of Mississippi, Royal Belgian Institute of Natural Sciences ( RBINS ), University of New Mexico, Instituto Nacional de Pesquisas da Amazônia, Centre de Biologie pour la Gestion des Populations ( CBGP ), Centre de Coopération Internationale en Recherche Agronomique pour le Développement ( CIRAD ) -Centre international d'études supérieures en sciences agronomiques ( Montpellier SupAgro ) -Institut national de la recherche agronomique [Montpellier] ( INRA Montpellier ) -Université de Montpellier ( UM ) -Institut de Recherche pour le Développement ( IRD [France-Sud] ) -Institut national d’études supérieures agronomiques de Montpellier ( Montpellier SupAgro ), Federal Agency for the Safety of the Food Chain, Ceríaco, Luis M., Gutiérrez, Eliécer, Dubois, Alan Alqarni, Abdulaziz, Buckup, Paulo, Simón Abdala, Cristian, Algarni, abdulaziz, A. Adriano, Edson, Erna, Aescht, Villanova Univ, Museu Nacl Hist Nat & Ciencia, Universidade de Brasília (UnB), Smithsonian Inst, Sorbonne Univ, Univ Nacl Tucuman, King Saud Univ, Cornell Univ, Universidade Federal de São Paulo (UNIFESP), Upper Austrian Museum, Univ Salzburg, Zool Forsch Museum A Koenig, Russian Acad Sci, Pontificia Univ Catolica Rio Grande do Sul, Museo Reg Sci Nat, Univ Fed Rio Grande do Sul, Royal Museum Cent Africa, Univ Nacl Autonoma Mexico, Univ Innsbruck, Hasselt Univ, Universidade Federal de Pernambuco (UFPE), Univ Nacl Misiones, Natl Museum, Senckenberg Nat Hist Sammlungen, Universidade Estadual Paulista (Unesp), Stephen F Austin State Univ, Landesmuseum, Univ Camerino, Universidade Federal do ABC (UFABC), Universidade Estadual de Londrina (UEL), Senckenberg Res Inst, Museo Civ Storia Nat, Polskiej Akad Nauk, Harvard Univ, North West Univ, Museu Nacl, Nat Hist Museum, Senckenberg Nat Kundemuseum, North Carolina Museum Nat Sci, Univ Rennes 1, Amer Museum Nat Hist, Univ Huddersfield, North Dakota State Univ, Fac Sci Sfax, DPTC PC, Louisiana State Univ, Zool Survey India, Univ Tennessee, Ohio State Univ, Univ Luterana Brasil, Univ Firenze, Univ Ovidius Constanta, Univ Mississippi, Royal Belgian Inst Nat Sci, Univ New Mexico, Inst Nacl de Pesquisas da Amazonia, Univ Stellenbosch, Universidade Federal de Minas Gerais (UFMG), CIBIO Ctr Invest Biodiversidade & Recursos Genet, Museum Hist Nat, Universidade Federal de Mato Grosso do Sul (UFMS), Univ Austral Chile, Univ Malaysia, Indian Inst Sci, Scottish Assoc Marine Sci, Sam Houston State Univ, Museo Nacl Ciencias Nat, Drexel Univ, Univ Richmond, Ctr Pesquisas Cacau, Univ Torino, Soc Hist Nat Alcide dOrbigny, Wolfden Sci Consulting, Universidade Federal do Rio de Janeiro (UFRJ), Inst Humboldt, Escuela Politec Nacl, Calif Acad Sci, Museum Dept Hist Nat Var, Nat Environm & Wildlife Soc, Univ Kansas, Kyoto Univ, Consejo Nacl Invest Cient & Tecn, Univ Fed Paraiba, Univ Fed Parana, Nat Hist Museum Narodini Museum, Nat Hist Museum Zimbabwe, Ateneo Manila Univ, Pontificia Univ Javeriana, RACINE, Univ Paris 06, Ocean Univ China, Museo Nacl Hist Nat Paraguay, Univ Carabobo, Natl Ctr Biol Sci, Univ Nebraska, CENAK Ctr Nat Kunde, Ohio Hist Connect, Univ Fed Amazonas, Univ Malaga, Chinese Acad Sci, Benemerita Univ Autonoma Puebla, Natl Polytech Inst, Field Museum Nat Hist, Univ Texas El Paso, Universidade Federal de Lavras (UFLA), La Sierra Univ, Univ San Francisco Quito, Univ Hong Kong, CUNY, CAS, Zool Staatssammlung Munchen, Univ Michigan, Helmholtz Ctr Environm Res, Santa Rosa Jr Coll, George Washington Univ, Inst Teknol Bandung, Earlham Coll, Senckenberg Forschungsinst & Nat Museum, Univ Koblenz Landau, Ditsong Natl Museum Nat Hist, Bombay Nat Hist Soc, Philipps Univ Marburg, Hess Landesmuseum, Smithsonian Conservat Biol Inst, Univ Pannonia, Staatliches Museum Nat Kunde, UFT, Museum Nat Kunde, Nat Hist Riksmuseet, Hikarigaoka, Inst Agr & Environm Sci, Univ Los Andes, Bat Jungle, Texas A&M Univ, Yale Univ, Museo Nacl Hist Nat, Roger Tory Peterson Inst Nat Hist, Univ Fed Reconcavo Bahia, South China Normal Univ, Museu Ciencias Tecnol PUCRS, Univ Fed Tocantins, Univ Toronto, Univ Nacl Colombia, Natl Museum Philippines, NOVA Univ Lisbon, Royal Saskatchewan Museum, Deutsch Zentrum Marine Biodiversitatsforsch, Ctr Invest Biol Noroeste, Naturalis Biodivers Ctr, Univ Douala, Vet & Farmaceut Univ Brno, Univ Swaziland, Fundacao Oswaldo Cruz, Univ Claude Bernard, Museum Vertebrate Zool, Nat Hist Museum & Inst, Senckenberg Biodiversitat & Klima Forschunsgzentr, Marquette Univ, Universidade Federal da Bahia (UFBA), Fed Univ ABC, Universidade Federal de Goiás (UFG), Univ Alaska Museum, Egerton Univ, Museo Hist Nat, IKIAM Univ Reg Amazon, Univ Hyogo, Inst Rech Dev, Niedersachs Landesbetrieb Wasserwirtschaft Kusten, Univ Complutense Madrid, Roosevelt Univ, Western Kentucky Univ, Univ Naples Federico II, Univ Fed Acre, Universidade Federal de Santa Catarina (UFSC), Inst Bio & Geociencias Noroeste Argentino, Inst Venezolano Invest Cient, Umweltbundesamt, Penclen, CNRS MNHN UPMC, Staatl Museum Nat Kunde, Ctr Biol Gest Populat INRA, Loyola Univ Chicago, Pontificia Univ Catolica Rio do Sul, Durban Museum Nat Sci, Univ Estado Amazonas, Brigham Young Univ, Museo Hist Nat La Salle, Univ Vet Med Hannover, Australian Museum, Texas Tech Univ, Francis Marion Univ, Univ Washington, Univ Connecticut, Universidade Federal de Uberlândia (UFU), Fed Univ Para, Yale Peabody Museum, Univ Texas Arlington, Senckenberg Deutsch Entomol Inst, CSIRO, Univ Venda, Univ Ulsan, Senckenberg Forsch Inst & Nat Museum, Univ Fed Para, UPMC, Tech Univ Kaiserslautern, Charles Univ Prague, Univ Nacl Expt los Llanos Occident Ezequiel Zamor, Long Isl Univ, Univ Ryukyus, Pontificia Univ Catolica Ecuador, Indiana Univ Penn, IRD, State Museum Nat Hist Karlsruhe, Univ Toulouse III Paul Sabatier, Univ Ghent, Univ Antwerp, Karlsruhe Inst Technol, Ctr Cellular & Mol Biol, Yale Peabody Museum Nat Hist, Bethune Cookman Univ, Natl Museum Nat & Sci, and Zool Garten Koln
Subjects: 0106 biological sciences, [SDV]Life Sciences [q-bio], Rebuttal, 010607 zoology, Biology, [SDV.BID.SPT]Life Sciences [q-bio]/Biodiversity/Systematics, Phylogenetics and taxonomy, Q1, 010603 evolutionary biology, 01 natural sciences, Biological Science Disciplines, FOTOGRAFIA, Photography, Animals, Animal species, Biological sciences, QH426, Ecology, Evolution, Behavior and Systematics, ComputingMilieux_MISCELLANEOUS, Taxonomy, QL, [ SDV ] Life Sciences [q-bio], Ecology, [SDV.BA]Life Sciences [q-bio]/Animal biology, Biodiversity, Classification, Ecology, Evolution, Behavior and Systematic, Taxonomy (biology), Animal Science and Zoology, Classics
Abstract: Made available in DSpace on 2021-06-25T12:17:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-11-23 Villanova Univ, Dept Biol, Villanova, PA 19085 USA Museu Nacl Hist Nat & Ciencia, Lisbon, Portugal Univ Brasilia, Dept Zool, Inst Ciencias Biol, BR-70910900 Brasilia, DF, Brazil Smithsonian Inst, Natl Museum Nat Hist, Washington, DC 20560 USA Sorbonne Univ, Museum Natl Hist Nat, ISYEB, Paris, France Univ Nacl Tucuman, San Miguel De Tucuman, Argentina King Saud Univ, Riyadh, Saudi Arabia Cornell Univ, Ithaca, NY USA Univ Fed Sao Paulo, Diadema, Brazil Upper Austrian Museum, Ctr Biol, Linz, Austria Villanova Univ, Villanova, PA 19085 USA Univ Salzburg, Salzburg, Austria Plazi, Bern, Switzerland Univ Brasilia, Brasilia, DF, Brazil Univ Sao Paulo, Ribeirao Preto, Brazil Zool Forsch Museum A Koenig, Bonn, Germany Museu Paraense Emilio Goeldi, Belem, Para, Brazil Russian Acad Sci, St Petersburg, Russia Pontificia Univ Catolica Rio Grande do Sul, Porto Alegre, RS, Brazil Museo Reg Sci Nat, Turin, Italy Univ Fed Rio Grande do Sul, Porto Alegre, RS, Brazil Royal Museum Cent Africa, Tervuren, Belgium Univ Nacl Autonoma Mexico, Mexico City, DF, Mexico Univ Innsbruck, Inst Ecol, Innsbruck, Austria Hasselt Univ, Hasselt, Belgium Univ Fed Pernambuco, Recife, PE, Brazil Univ Fed Espirito Santo, Vitoria, ES, Brazil Univ Nacl Misiones, Felix De Azara, Argentina Albany Museum, Grahamstown, South Africa Natl Museum, Bloemfontein, South Africa Senckenberg Nat Hist Sammlungen, Dresden, Germany Univ Estadual Paulista, Botucatu, SP, Brazil Stephen F Austin State Univ, Nacogdoches, TX 75962 USA Landesmuseum, Leonding, Austria Univ Camerino, Camerino, Italy Univ Fed ABC, Sao Bernardo, Brazil Univ Estadual Londrina, Museu Zool, Londrina, Parana, Brazil Senckenberg Res Inst, Frankfurt, Germany Museo Civ Storia Nat, Carmagnola, Italy Polskiej Akad Nauk, Muzeum & Inst Zool, Warsaw, Poland Museo Civ Storia Nat, Genoa, Italy Russian Acad Sci, Inst Zool, St Petersburg, Russia Univ Estadual Paulista, Sao Vicente, Brazil Sorbonne Univ, ISYEB, Museum Natl Hist Nat, Paris, France Port Elizabeth Museum, Port Elizabeth, South Africa Sam Noble Museum, Norman, OK USA Harvard Univ, Museum Comparat Zool, Cambridge, MA 02138 USA North West Univ, Potchefstroom, South Africa Museu Nacl, Rio De Janeiro, Brazil Nat Hist Museum, Basel, Switzerland Senckenberg Nat Kundemuseum, Gorlitz, Germany North Carolina Museum Nat Sci, Raleigh, NC USA Univ Rennes 1, Rennes, France Univ Sao Paulo, Museu Zool, Sao Paulo, SP, Brazil Amer Museum Nat Hist, New York, NY 10024 USA Univ Huddersfield, Huddersfield, W Yorkshire, England North Dakota State Univ, Fargo, ND USA Univ Sao Paulo, Sao Paulo, SP, Brazil Fac Sci Sfax, Sfax, Tunisia DPTC PC, Manaus, Amazonas, Brazil Louisiana State Univ, Museum Nat Sci, Baton Rouge, LA 70803 USA Zool Survey India, Kolkata, India Univ Tennessee, Chattanooga, TN USA Ohio State Univ, Columbus, OH 43210 USA Univ Luterana Brasil, Museu Ciencias Nat, Canoas, Brazil Univ Firenze, Florence, Italy Univ Ovidius Constanta, Constanta, Romania Univ Mississippi, Oxford, MS USA Royal Belgian Inst Nat Sci, Brussels, Belgium Univ New Mexico, Albuquerque, NM 87131 USA Inst Nacl de Pesquisas da Amazonia, Manaus, Amazonas, Brazil Univ Stellenbosch, Matieland, South Africa Univ Fed Minas Gerais, Belo Horizonte, MG, Brazil CIBIO Ctr Invest Biodiversidade & Recursos Genet, Vairao, Portugal Museum Hist Nat, Geneva, Switzerland Univ Fed Mato Grosso, Cuiaba, Brazil Univ Austral Chile, Valdivia, Chile Univ Malaysia, Sarawak, Malaysia Indian Inst Sci, Bangalore, Karnataka, India Scottish Assoc Marine Sci, Oban, Argyll, Scotland Sam Houston State Univ, Huntsville, TX 77340 USA Museo Nacl Ciencias Nat, Madrid, Spain Sorbonne Univ, CR2P, Museum Natl Hist Nat, Paris, France Drexel Univ, Acad Nat Sci, Philadelphia, PA 19104 USA Univ Richmond, Richmond, VA 23173 USA Ctr Pesquisas Cacau, Itabuna, Brazil Univ Torino, Turin, Italy Soc Hist Nat Alcide dOrbigny, Aubiere, France Wolfden Sci Consulting, Murcia, Spain Univ Fed Rio de Janeiro, Macae, Brazil Univ Tennessee, Knoxville, TN USA Inst Humboldt, Villa De Leyva, Colombia Escuela Politec Nacl, Quito, Ecuador Calif Acad Sci, San Francisco, CA 94118 USA Museum Dept Hist Nat Var, Toulon, France Nat Environm & Wildlife Soc, Angul, India Univ Kansas, Lawrence, KS 66045 USA Kyoto Univ, Kyoto, Japan Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivadavia, Buenos Aires, DF, Argentina Univ Fed Paraiba, Joao Pessoa, Paraiba, Brazil Univ Fed Parana, Curitiba, Parana, Brazil Univ Fed Rio de Janeiro, Rio De Janeiro, Brazil Nat Hist Museum Narodini Museum, Prague, Czech Republic Nat Hist Museum Zimbabwe, Bulawayo, Zimbabwe Ateneo Manila Univ, Quezon City, Philippines Pontificia Univ Javeriana, Bogota, Colombia RACINE, St Maugan, France Univ Paris 06, Sorbonne Univ Paris, ISYEB, Paris, France Ocean Univ China, Qingdao, Peoples R China Museo Nacl Hist Nat Paraguay, San Lorenzo, Paraguay Univ Carabobo, Valencia, Venezuela Natl Ctr Biol Sci, Bengaluru, India Univ Nebraska, Lincoln, NE USA CENAK Ctr Nat Kunde, Hamburg, Germany Ohio Hist Connect, Columbus, OH USA Univ Fed Amazonas, Manaus, Amazonas, Brazil Univ Malaga, Malaga, Spain Chinese Acad Sci, Qingdao, Shandong, Peoples R China Benemerita Univ Autonoma Puebla, Puebla, Mexico Natl Polytech Inst, Ctr Res & Adv Studies, Irapuato, Mexico Field Museum Nat Hist, Chicago, IL 60605 USA Univ Texas El Paso, El Paso, TX 79968 USA Univ Fed Lavras, Lavras, Brazil Nat Hist Museum, Vienna, Austria La Sierra Univ, Riverside, CA USA Univ San Francisco Quito, Quito, Ecuador Univ Hong Kong, Hong Kong, Peoples R China CUNY, New York, NY 10021 USA CAS, Inst Vertebrate Biol, Brno, Czech Republic Sorbonne Univ, MECADEV, Museum Natl Hist Nat, Paris, France Univ Estadual Paulista, Rio Claro, Brazil Zool Staatssammlung Munchen, Munich, Germany Univ Michigan, Dearborn, MI 48128 USA Smithsonian Inst, Natl Museum Nat Hist, Washington, DC USA Helmholtz Ctr Environm Res, Leipzig, Germany Santa Rosa Jr Coll, Santa Rosa, CA USA George Washington Univ, Washington, DC 20037 USA Inst Teknol Bandung, Bandung, Indonesia Earlham Coll, Richmond, IN USA Senckenberg Forschungsinst & Nat Museum, Frankfurt, Germany Univ Estadual Londrina, Londrina, Parana, Brazil Univ Koblenz Landau, Koblenz, Germany Nat Hist Museum, London, England Ditsong Natl Museum Nat Hist, Pretoria, South Africa Bombay Nat Hist Soc, Bombay, Maharashtra, India Philipps Univ Marburg, Marburg, Germany Hess Landesmuseum, Darmstadt, Germany Smithsonian Conservat Biol Inst, Washington, DC USA Univ Pannonia, Keszthely, Hungary Staatliches Museum Nat Kunde, Stuttgart, Germany UFT, Tocantins, Portugal Museum Nat Kunde, Berlin, Germany Nat Hist Riksmuseet, Stockholm, Sweden Hikarigaoka, Munakata, Japan Inst Agr & Environm Sci, Tartu, Estonia Univ Los Andes, Merida, Venezuela Bat Jungle, Monteverde, Costa Rica Texas A&M Univ, College Stn, TX USA Yale Univ, New Haven, CT USA Univ Estadual Paulista, Sao Jose Do Rio Preto, Brazil Museo Nacl Hist Nat, Montevideo, Uruguay Consejo Nacl Invest Cient & Tecn, Fdn Miguel Lillo, San Miguel De Tucuman, Argentina Roger Tory Peterson Inst Nat Hist, Jamestown, VA USA Univ Fed Reconcavo Bahia, Cruz Das Almas, Brazil South China Normal Univ, Guangzhou 510631, Guangdong, Peoples R China Museu Ciencias Tecnol PUCRS, Porto Alegre, RS, Brazil Univ Fed Tocantins, Porto Nacl, Brazil Univ Toronto, Scarborough, ON, Canada Univ Nacl Colombia, Inst Ciencias Nat, Bogota, Colombia Natl Museum Philippines, Manila, Philippines NOVA Univ Lisbon, Caparica, Portugal Royal Saskatchewan Museum, Regina, SK, Canada Deutsch Zentrum Marine Biodiversitatsforsch, Hamburg, Germany Ctr Invest Biol Noroeste, La Paz, Mexico Naturalis Biodivers Ctr, Leiden, Netherlands Univ Douala, Douala, Cameroon Vet & Farmaceut Univ Brno, Brno, Czech Republic Univ Swaziland, Kwaluseni, Eswatini Fundacao Oswaldo Cruz, Rio De Janeiro, Brazil Univ Claude Bernard, Lyon, France Museum Vertebrate Zool, Berkeley, CA USA Smithsonian Inst, Washington, DC 20560 USA Nat Hist Museum & Inst, Chiba, Japan Senckenberg Biodiversitat & Klima Forschunsgzentr, Frankfurt, Germany Marquette Univ, Milwaukee, WI 53233 USA Univ Firenze, Museo Storia Nat, Florence, Italy Univ Fed Bahia, Salvador, BA, Brazil Fed Univ ABC, Sao Bernardo, Brazil Univ Fed Goias, Goiania, Go, Brazil Univ Alaska Museum, Fairbanks, AK USA Egerton Univ, Egerton, Kenya Museo Hist Nat, Lima, Peru IKIAM Univ Reg Amazon, Tena, Ecuador Univ Hyogo, Sanda, Japan Inst Rech Dev, Paris, France Univ Sao Paulo, Piracicaba, Brazil Niedersachs Landesbetrieb Wasserwirtschaft Kusten, Hannover, Germany Univ Complutense Madrid, Madrid, Spain Consejo Nacl Invest Cient & Tecn, Ctr Nacl Patagon, Puerto Madryn, Argentina Roosevelt Univ, Coll Profess Studies, Chicago, IL 60605 USA Western Kentucky Univ, Bowling Green, KY 42101 USA Univ Naples Federico II, Naples, Italy Univ Fed Acre, Rio Branco, Acre, Brazil Univ Fed Santa Catarina, Florianopolis, SC, Brazil Inst Bio & Geociencias Noroeste Argentino, Salta, Argentina Inst Venezolano Invest Cient, Caracas, Venezuela Umweltbundesamt, Vienna, Austria Penclen, Plumelec, France CNRS MNHN UPMC, Ctr Rech Paleobiodivers & Paleoenvironm, Paris, France Staatl Museum Nat Kunde, Stuttgart, Germany Consejo Nacl Invest Cient & Tecn, Museo Argentino Ciencias Nat Bernardino Rivada, Buenos Aires, DF, Argentina Ctr Biol Gest Populat INRA, Montferrier Sur Lez, France Loyola Univ Chicago, Chicago, IL USA Pontificia Univ Catolica Rio do Sul, Porto Alegre, RS, Brazil Durban Museum Nat Sci, Durban, South Africa Univ Estado Amazonas, Manaus, Amazonas, Brazil Brigham Young Univ, Provo, UT 84602 USA Museo Hist Nat La Salle, Caracas, Venezuela Univ Vet Med Hannover, Hannover, Germany Australian Museum, Sydney, NSW, Australia Texas Tech Univ, Lubbock, TX 79409 USA Francis Marion Univ, Florence, SC USA Univ Washington, Seattle, WA 98195 USA Univ Connecticut, Groton, CT USA Fed Univ ABC, Santo Andre, SP, Brazil Univ Fed Uberlandia, Uberlandia, MG, Brazil Fed Univ Para, Belem, Para, Brazil Yale Peabody Museum, New Haven, CT USA Univ Texas Arlington, Arlington, TX 76019 USA Senckenberg Deutsch Entomol Inst, Muncheberg, Germany CSIRO, Natl Res Collect, Canberra, ACT, Australia Univ Venda, Thohoyandou, South Africa Univ Ulsan, Ulsan, South Korea Univ Estadual Sao Paulo, Botucatu, SP, Brazil Senckenberg Forsch Inst & Nat Museum, Frankfurt, Germany Ocean Univ China, Inst Marine Biodivers & Evolut, Qingdao, Shandong, Peoples R China Univ Fed Para, Altamira, Brazil Univ Estadual Paulista, Assis, Brazil UPMC, Ctr Rech Paleobiodiversite & Paleoenvironm, CNRS, MNHN, Paris, France Tech Univ Kaiserslautern, Kaiserslautern, Germany Charles Univ Prague, Dept Zool, Prague, Czech Republic Univ Nacl Expt los Llanos Occident Ezequiel Zamor, Guanare, Venezuela Long Isl Univ, Brooklyn, NY USA Univ Ryukyus, Okinawa, Japan Pontificia Univ Catolica Ecuador, Museo Zool, Escuela Ciencias Biol, Quito, Ecuador Indiana Univ Penn, Indiana, PA USA IRD, Dakar, Senegal State Museum Nat Hist Karlsruhe, Karlsruhe, Germany Univ Toulouse III Paul Sabatier, Toulouse, France Univ Ghent, Ghent, Belgium Univ Antwerp, Antwerp, Belgium Karlsruhe Inst Technol, Karlsruhe, Germany Ctr Cellular & Mol Biol, Hyderabad, India Univ Fed ABC, Santo Andre, Brazil Yale Peabody Museum Nat Hist, New Haven, CT USA Univ Innsbruck, Mondsee, Austria Bethune Cookman Univ, Daytona Beach, FL USA Natl Museum Nat & Sci, Tokyo, Japan Zool Garten Koln, Cologne, Germany Univ Estadual Paulista, Botucatu, SP, Brazil Univ Estadual Paulista, Sao Vicente, Brazil Univ Estadual Paulista, Rio Claro, Brazil Univ Estadual Paulista, Sao Jose Do Rio Preto, Brazil Univ Estadual Paulista, Assis, Brazil
Published: 2016
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14. Genome sequence of Aedes aegypti, a major arbovirus vector

Author: Sergio Verjovski-Almeida, James E. Galagan, Ryan C. Kennedy, Zhiyong Xi, Jason R. Miller, Eric Eisenstadt, Kyanne R. Reidenbach, Robert V. Bruggner, Yu-Hui Rogers, Hadi Quesneville, Doreen Werner, Owen White, Alexander S. Raikhel, Mario Stanke, J. Spencer Johnston, Diane D. Lovin, Evgenia V. Kriventseva, Ian T. Paulsen, Kathryn S. Campbell, Norman H. Lee, Ewan Birney, Karyn Megy, Hean Koo, David Kulp, Shelby L. Bidwell, Jingsong Zhu, Philip Montgomery, Paolo Amedeo, Yongmei Zhao, Chinnappa D. Kodira, Javier Costas, Michael C. Schatz, Steven P. Sinkins, Claire M. Fraser-Liggett, Martin Shumway, Kurt LaButti, Akio Mori, Brendan J. Loftus, Manfred Grabherr, Eric O. Stinson, Frank H. Collins, Zhijian Jake Tu, Monique R. Coy, Matt Crawford, Janice P. Vanzee, William M. Gelbart, Joshua Orvis, Peter Arensburger, Chunhong Mao, Evgeny M. Zdobnov, Saul A. Kravitz, Suely Lopes Gomes, David DeCaprio, David G. Hogenkamp, Daniel Lawson, Dennis L. Knudson, David W. Severson, George Dimopoulos, Marcelo B. Soares, Sinéad B. O'Leary, Peter W. Atkinson, David M. Jaffe, Becky deBruyn, Martin Hammond, Ana L. T. O. Nascimento, Jim Biedler, Stefan Wyder, Jose M. C. Tubio, Bruce W. Birren, Catherine A. Hill, Chad Nusbaum, Eduardo Lee, Song Li, Susan E. Brown, Jennifer R. Wortman, James R. Hogan, Hamza El-Dorry, Qi Zhao, Linda Hannick, Carlos Frederico Martins Menck, Vishvanath Nene, Jonathan Crabtree, Steven L. Salzberg, Michael H. Holmes, Maria de Fatima Bonaldo, Quinghu Ren, Mihaela Pertea, Charles Roth, Evan Mauceli, Karin Eiglmeier, Horacio Naveira, Brian J. Haas, Qiandong Zeng, Neil F. Lobo, Jennifer R. Schneider, The Institute for Genomic Research, The Institute for Genomic Research, Rockville, European Bioinformatics Institute [Hinxton] ( EMBL-EBI ), European Molecular Biology Laboratory [Hinxton], Broad Institute of MIT and Harvard ( BROAD INSTITUTE ), Broad Institute of MIT and Harvard, Virginia Polytechnic Institute and State University [Blacksburg], University College Dublin [Dublin] ( UCD ), Bloomberg School of Public Health, Johns Hopkins University ( JHU ) -Bloomberg School of Public Health, University of Geneva Medical School, Swiss Institute of Bioinformatics-University of Geneva Medical School, University of Notre Dame ( UND ), Harvard University [Cambridge], College of Agricultural Sciences Colorado State University, Colorado State University [Fort Collins] ( CSU ) -College of Agricultural Sciences, Northwestern University [Evanston], University of California [Riverside] ( UCR ), Department of Atmospheric, Oceanic and Planetary Physics [Oxford] ( AOPP ), University of Oxford [Oxford], Purdue University [West Lafayette], Centro Nacional de Genotipado Fundación Pública Galega de Medicina Xenómica Hospital Clínico Universitario de Santiago, Centro Nacional de Genotipado-Fundación Pública Galega de Medicina Xenómica-Hospital Clínico Universitario de Santiago, Institut Pasteur [Paris], Universidade de Sao Paulo Instituto de Quimica, Universidade de São Paulo ( USP ) -Instituto de Quimica, Texas A&M University [College Station], Joint Technology Center, University of Massachusetts [Amherst] ( UMass Amherst ), Universidade de Sao Paulo, Institute of Biomedical Sciences, Universidade de São Paulo ( USP ) -Institute of Biomedical Sciences, Instituto Butantan [São Paulo], Universidade da Coruña, University of Maryland [College Park], Institut Jacques Monod ( IJM ), Université Paris Diderot - Paris 7 ( UPD7 ) -Centre National de la Recherche Scientifique ( CNRS ), University of California [Santa Cruz] ( UCSC ), Complexo Hospitalario Universitario de Santiago, Universität Göttingen, Georg-August-Universität Göttingen, George Washington University Medical Center, George Washington University ( GW ), The Institute for Genomic Research (TIGR), European Bioinformatics Institute [Hinxton] (EMBL-EBI), EMBL Heidelberg, Broad Institute of MIT and Harvard (BROAD INSTITUTE), Harvard Medical School [Boston] (HMS)-Massachusetts Institute of Technology (MIT)-Massachusetts General Hospital [Boston], University College Dublin [Dublin] (UCD), Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU), Swiss Institute of Bioinformatics [Lausanne] (SIB), Université de Lausanne (UNIL)-Université de Lausanne (UNIL)-University of Geneva Medical School, University of Notre Dame [Indiana] (UND), Colorado State University [Fort Collins] (CSU)-College of Agricultural Sciences, University of California [Riverside] (UCR), University of California, Department of Atmospheric, Oceanic and Planetary Physics [Oxford] (AOPP), Universidade de São Paulo (USP)-Instituto de Quimica, University of Massachusetts [Amherst] (UMass Amherst), University of Massachusetts System (UMASS), Universidade de São Paulo (USP)-Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP), University of Maryland System, Institut Jacques Monod (IJM (UMR_7592)), Université Paris Diderot - Paris 7 (UPD7)-Centre National de la Recherche Scientifique (CNRS), University of California [Santa Cruz] (UCSC), Georg-August-University [Göttingen], The George Washington University (GW), George Washington University (GW), Université de Lausanne = University of Lausanne (UNIL)-Université de Lausanne = University of Lausanne (UNIL)-University of Geneva Medical School, Harvard University, University of California [Riverside] (UC Riverside), University of California (UC), University of Oxford, Institut Pasteur [Paris] (IP), Universidade de São Paulo = University of São Paulo (USP)-Instituto de Quimica, Universidade de São Paulo = University of São Paulo (USP)-Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo = University of São Paulo (USP), University of California [Santa Cruz] (UC Santa Cruz), Georg-August-University = Georg-August-Universität Göttingen, Zdobnov, Evgeny, and Wyder, Stefan
Subjects: 0106 biological sciences, Male, Transcription, Genetic, Genome, Insect, transposons, receptors, Genes, Insect, Aedes/ genetics/metabolism, MESH: Genes, Insect, Yellow Fever/prevention & control/transmission, MESH: Base Sequence, 01 natural sciences, Dengue/prevention & control/transmission, MESH: Protein Structure, Tertiary, MESH: Arboviruses, MESH : Insect Vectors, MESH: Insect Proteins, MESH : Anopheles gambiae, MESH: Animals, MESH : Arboviruses, insects, MESH: Yellow Fever, superfamily, ddc:616, 0303 health sciences, Anopheles, MESH : Genes, Insect, 3. Good health, yellow-fever mosquito, anopheles-gambiae, drosophila-melanogaster, expression, evolution, organization, Drosophila melanogaster, MESH: DNA Transposable Elements, [ SDV.BBM.GTP ] Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], Multigene Family, Public Health, MESH : Protein Structure, Tertiary, MESH: Sex Characteristics, Molecular Sequence Data, MESH : Multigene Family, MESH: Sex Determination (Genetics), MESH : Sex Determination (Genetics), Arbovirus, Article, 03 medical and health sciences, Species Specificity, [SDV.BBM.GTP]Life Sciences [q-bio]/Biochemistry, Molecular Biology/Genomics [q-bio.GN], MESH: Anopheles gambiae, Yellow Fever, MESH: Species Specificity, Humans, MESH: Humans, MESH: Molecular Sequence Data, fungi, MESH : Humans, MESH : Sex Characteristics, Membrane Transport Proteins, Sex Determination Processes, medicine.disease, Insect Vectors, Protein Structure, Tertiary, Sex Determination (Genetics), MESH: Multigene Family, MESH: Female, MESH : Sequence Analysis, DNA, MESH: Sequence Analysis, DNA, Drosophila melanogaster/genetics, MESH : Molecular Sequence Data, Odorant binding, Insect Proteins/genetics, Anopheles gambiae, MESH: Dengue, Genome, MESH: Membrane Transport Proteins, Dengue, MESH : Membrane Transport Proteins, Aedes, Insect Vectors/ genetics/metabolism, MESH : Drosophila melanogaster, MESH : Female, Membrane Transport Proteins/genetics, Genetics, MESH : Insect Proteins, Sex Characteristics, Multidisciplinary, MESH: Synteny, MESH: Aedes, MESH : Genome, Insect, MESH : DNA Transposable Elements, Insect Proteins, Female, Orthologous Gene, MESH : Male, MESH : Dengue, Aedes aegypti, MESH: Insect Vectors, Biology, 010603 evolutionary biology, Synteny, MESH: Drosophila melanogaster, Protein Structure, Tertiary/genetics, MESH : Yellow Fever, parasitic diseases, medicine, MESH : Species Specificity, Animals, 030304 developmental biology, MESH : Aedes, Base Sequence, MESH: Genome, Insect, MESH: Transcription, Genetic, MESH : Synteny, MESH : Transcription, Genetic, Sequence Analysis, DNA, biology.organism_classification, MESH: Male, DNA Transposable Elements, MESH : Base Sequence, MESH : Animals, Arboviruses, Anopheles gambiae/genetics/metabolism
Abstract: We present a draft sequence of the genome of Aedes aegypti , the primary vector for yellow fever and dengue fever, which at ∼1376 million base pairs is about 5 times the size of the genome of the malaria vector Anopheles gambiae . Nearly 50% of the Ae. aegypti genome consists of transposable elements. These contribute to a factor of ∼4 to 6 increase in average gene length and in sizes of intergenic regions relative to An. gambiae and Drosophila melanogaster . Nonetheless, chromosomal synteny is generally maintained among all three insects, although conservation of orthologous gene order is higher (by a factor of ∼2) between the mosquito species than between either of them and the fruit fly. An increase in genes encoding odorant binding, cytochrome P450, and cuticle domains relative to An. gambiae suggests that members of these protein families underpin some of the biological differences between the two mosquito species.
Published: 2016
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15. Worldwide sequence conservation of transmission-blocking vaccine candidate Pvs230 in Plasmodium vivax

Author: Kazuyuki Tanabe, Jetsumon Sattabongkot, Shin-Ichiro Tachibana, Masanori Yagi, Meiko Hamai, Marcelo U. Ferreira, Takafumi Tsuboi, Hiroshi Ohmae, Toshihiro Horii, Masanori Doi, Yaming Cao, Milijaona Randrianarivelojosia, Akira Kaneko, Toshihiro Mita, Motomi Torii, Mayumi Tachibana, Fadile Yildiz Zeyrek, Cell-free Science and Technology Research Center, Ehime University [Matsuyama], Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University [Osaka], Department of Molecular Parasitology, Ehime University Graduate School of Medicine, Department of International Affairs and Tropical Medicine, Tokyo Women's Medical University (TWMU), Department of Molecular Protozoology, Research Institute for Microbial Diseases, Department of Microbiology, Harran University Medical Faculty, Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Department of Parasitology, National Institute of Infectious Diseases, Karolinska Institutet [Stockholm], Global COE, Nagasaki University, Institut Pasteur de Madagascar, Réseau International des Instituts Pasteur (RIIP), Armed Forces Research Institute of Medical Sciences [Bangkok] (AFRIMS), Department of Immunology, College of Basic Medical Sciences, China Medical University, Ehime Proteo-Medicine Research Center, Venture Business Laboratory, and This research was supported by the Ministry of Education, Culture, Sports, Science and Technology (18073013, 18GS03140013, 20390120, 19406009, 21022034, 22406012), and by the Ministry of Health, Labour, and Welfare, Japan (H20-Shinkou-ippan-013, H21-Chikyukibo-ippan-005) and by Japan Society for the Promotion of Science Fellowship Program (to FYZ). Field work in Brazil was funded by the National Institutes of Health of USA (RO1 AI 075416-01), the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, 470570/2006-7), and the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP, 05/51988-0 and 07/51199-0). This work was also supported by grant from the National Natural Science Foundation of China (30972774)
Subjects: MESH: Sequence Analysis, DNA, [SDV]Life Sciences [q-bio], Plasmodium vivax, Protozoan Proteins, MESH: Amino Acid Sequence, Nucleotide diversity, Conserved sequence, 0302 clinical medicine, MESH: Animals, MESH: Phylogeny, MESH: Protozoan Proteins, Conserved Sequence, Phylogeny, Genetics, 0303 health sciences, education.field_of_study, MESH: Conserved Sequence, biology, MESH: Malaria Vaccines, MESH: Plasmodium vivax, 3. Good health, Infectious Diseases, Molecular Medicine, Sequence analysis, Molecular Sequence Data, 030231 tropical medicine, Population, MESH: Sequence Alignment, MESH: DNA, Protozoan, Antigens, Protozoan, Article, 03 medical and health sciences, Malaria Vaccines, parasitic diseases, Malaria, Vivax, medicine, Gametocyte, Animals, Humans, Amino Acid Sequence, education, 030304 developmental biology, MESH: Humans, MESH: Molecular Sequence Data, General Veterinary, General Immunology and Microbiology, Public Health, Environmental and Occupational Health, MESH: Malaria, Vivax, Plasmodium falciparum, Sequence Analysis, DNA, DNA, Protozoan, biology.organism_classification, medicine.disease, Virology, Sequence Alignment, Malaria, MESH: Antigens, Protozoan
Abstract: International audience; Pfs230, surface protein of gametocyte/gamete of the human malaria parasite, Plasmodium falciparum, is a prime candidate of malaria transmission-blocking vaccine. Plasmodium vivax has an ortholog of Pfs230 (Pvs230), however, there has been no study in any aspects on Pvs230 to date. To investigate whether Pvs230 can be a vivax malaria transmission-blocking vaccine, we performed evolutionary and population genetic analysis of the Pvs230 gene (pvs230: PVX_003905). Our analysis of Pvs230 and its orthologs in eight Plasmodium species revealed two distinctive parts: an interspecies variable part (IVP) containing species-specific oligopeptide repeats at the N-terminus and a 7.5kb interspecies conserved part (ICP) containing 14 cysteine-rich domains. Pvs230 was closely related to its orthologs, Pks230 and Pcys230, in monkey malaria parasites. Analysis of 113 pvs230 sequences obtained from worldwide, showed that nucleotide diversity is remarkably low in the non-repeat 8-kb region of pvs230 (θπ=0.00118) with 77 polymorphic nucleotide sites, 40 of which results in amino acid replacements. A signature of purifying selection but not of balancing selection was seen on pvs230. Functional and/or structural constraints may limit the level of polymorphism in pvs230. The observed limited polymorphism in pvs230 should ground for utilization of Pvs230 as an effective transmission-blocking vaccine.
Published: 2011
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16. CPDs and 6-4PPs play different roles in UV-induced cell death in normal and NER-deficient human cells

Author: Alain Sarasin, Melissa Gava Armelini, Gustavo P. Amarante-Mendes, Carlos Frederico Martins Menck, Vanessa Chiganças, Jacqueline F. Jacysyn, Keronninn M. Lima-Bessa, Génomes et cancer (GC (FRE2939)), Université Paris-Sud - Paris 11 (UP11)-Institut Gustave Roussy (IGR)-Centre National de la Recherche Scientifique (CNRS), Etude des relations instabilité génétique et cancer (ERIGC), Centre National de la Recherche Scientifique (CNRS), Universidade de Sao Paulo, Institute of Biomedical Sciences, Universidade de São Paulo (USP)-Institute of Biomedical Sciences (ICB/USP), and Universidade de São Paulo (USP)
Subjects: MESH: Cell Death, [SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT], MESH: Cell Line, Tumor, Xeroderma pigmentosum, Ultraviolet Rays, DNA damage, DNA repair, Immunoblotting, Receptors, Cytoplasmic and Nuclear, MESH: Flow Cytometry, Pyrimidine dimer, MESH: Receptors, Cytoplasmic and Nuclear, Biology, Biochemistry, Adenoviridae, 03 medical and health sciences, 0302 clinical medicine, Transduction, Genetic, Cell Line, Tumor, Ultraviolet light, medicine, Humans, skin and connective tissue diseases, Photolyase, Molecular Biology, Liver X Receptors, 030304 developmental biology, MESH: DNA Damage, 0303 health sciences, MESH: Humans, MESH: Pyrimidine Dimers, Cell Death, MESH: Immunoblotting, Skin photosensitivity, MESH: Adenoviridae, Cell Biology, Flow Cytometry, Orphan Nuclear Receptors, MESH: Transduction, Genetic, medicine.disease, Molecular biology, 3. Good health, Pyrimidine Dimers, 030220 oncology & carcinogenesis, MESH: Ultraviolet Rays, DNA Damage, Nucleotide excision repair
Abstract: Ultraviolet (UV) light generates two major DNA lesions: cyclobutane pyrimidine dimers (CPDs) and pyrimidine-(6-4)-pyrimidone photoproducts (6-4PPs), but the specific participation of these two lesions in the deleterious effects of UV is a longstanding question. In order to discriminate the precise role of unrepaired CPDs and 6-4PPs in UV-induced responses triggering cell death, human fibroblasts were transduced by recombinant adenoviruses carrying the CPD-photolyase or 6-4PP-photolyase cDNAs. Both photolyases were able to prevent UV-induced apoptosis in cells deficient for nucleotide excision repair (NER) to a similar extent, while in NER-proficient cells UV-induced apoptosis was prevented only by CPD-photolyase, with no effects observed when 6-4PPs were removed by the specific photolyase. These results strongly suggest that both CPDs and 6-4PPs contribute to UV-induced apoptosis in NER-deficient cells, while in NER-proficient cells, CPDs are the only lesions responsible for UV-killing, probably due to the rapid repair of 6-4PPs by NER. As a consequence, the difference in skin photosensitivity, including carcinogenesis, of most of the xeroderma pigmentosum patients and of normal people is probably not only a quantitative aspect, but depends on the type of DNA damage induced by sunlight and its rate of repair.
Published: 2008
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17. Evolution of the levels of human leukocyte antigen G (HLA-G) in Beninese infant during the first year of life in a malaria endemic area: using latent class analysis

Author: Kabirou Moutairou, Benoit Favier, Philippe Moreau, Tania d’Almeida, Ibrahim Sadissou, Gilles Cottrell, André Garcia, Nathalie Rouass-Freiss, Achille Massougbodji, Rachida Tahar, David Courtin, Eduardo Antônio Donadi, Université Pierre et Marie Curie - Paris 6 ( UPMC ), Mère et enfant face aux infections tropicales ( MERIT - UMR_D 216 ), Institut de Recherche pour le Développement ( IRD ) -Université Paris Descartes - Paris 5 ( UPD5 ), Université Paris Descartes - Paris 5 ( UPD5 ), Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et l'Enfance (CERPAGE) ( CERPAGE ), Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et l'Enfance (CERPAGE), Université d'Abomey Calavi, Institut des Maladies Emergentes et des Thérapies Innovantes ( IMETI ), Commissariat à l'énergie atomique et aux énergies alternatives ( CEA ) -Université Paris-Saclay, Universidade de São Paulo ( USP ), Université Pierre et Marie Curie - Paris 6 (UPMC), Mère et enfant face aux infections tropicales (MERIT - UMR_D 216), Institut de Recherche pour le Développement (IRD)-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Paris 5 (UPD5), Centre d'Etude et de Recherche sur le Paludisme Associé à la Grossesse et l'Enfance (CERPAGE) (CERPAGE), University of Abomey Calavi (UAC), Institut des Maladies Emergentes et des Thérapies Innovantes (IMETI), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Universidade de São Paulo (USP), Mère et enfant en milieu tropical : pathogènes, système de santé et transition épidémiologique (MERIT - UMR_D 216), Centre d’Etude et de Recherche sur le Paludisme Associé à la Grossesse et l’Enfance [Cotonou, Bénin] (CERPAGE), Université d’Abomey-Calavi = University of Abomey Calavi (UAC), Universidade de São Paulo = University of São Paulo (USP), and Centre d’Etude et de Recherche sur le Paludisme Associé à la Grossesse et l’Enfance (CERPAGE)
Subjects: 0301 basic medicine, Adult, Male, medicine.medical_specialty, Infancy, Adolescent, sHLA-G, Evolution, RECÉM-NASCIDO, Groups, Enzyme-Linked Immunosorbent Assay, Human leukocyte antigen, Biology, 03 medical and health sciences, Young Adult, Pregnancy, HLA-G, [ SDV.MHEP ] Life Sciences [q-bio]/Human health and pathology, parasitic diseases, medicine, Humans, Benin, HLA-G Antigens, Research, Infant, Newborn, Infant, Middle Aged, medicine.disease, Latent class model, 3. Good health, Malaria, Low birth weight, 030104 developmental biology, Infectious Diseases, Parasitology, Tropical medicine, Immunology, Female, Disease Susceptibility, medicine.symptom, sHLA‑G, [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology
Abstract: International audience; Background: HLA‑G, a non‑classical HLA class I antigen, is of crucial interest during pregnancy by inhibiting mater‑ nal immune response. Its role during infections is discussed, and it has been described that high levels of soluble HLA‑G during childhood increase the risk of malaria. To explore more precisely interactions between soluble HLA‑G and malaria, latent class analysis was used to test whether distinct sub‑populations of children, each with distinctive soluble HLA‑G evolutions may suggest the existence of groups presenting variable malaria susceptibility. Method: A study was conducted in Benin from 2010 to 2013 and 165 children were followed from birth to 12 months. Evolution of soluble HLA‑G was studied by the latent class method. Results: Three groups of children were identified: one with consistently low levels of soluble HLA‑G during follow‑up, a second with very high levels and a last intermediate group. In all groups, low birth weight, high number of malaria infections and high exposure to malaria transmission were associated with high level of soluble HLA‑G. Placental malaria was not. Presence of soluble HLA‑G in cord blood increased the probability of belonging to the highest trajectory. Conclusion: These results, together with previous ones, confirm the important role of HLA‑G in the individual sus‑ ceptibility to malaria. Assaying soluble HLA‑G at birth could be a good indicator of newborns more fragile and at risk of infections during childhood.
Published: 2016
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18. Climate seasonality limits leaf carbon assimilation and wood productivity in tropical forests

Author: Jürgen Homeier, Joseph J. O'Brien, Hellen Paredio Santana, Jean-Louis Devineau, Wendeson Castro, Claudio Sergio Lisi, Marcos Miranda Toledo, Grégoire Vincent, Bruno Hérault, Benjamin Brede, Martin Worbes, Raphaël Pélissier, Takeshi Toma, Aster Gebrekirstos, Vinicius Resende de Castro, Franziska Volland, Kaiyu Guan, Damien Bonal, Steven F. Oberbauer, Ervan Rutishauser, Davi Rodrigo Rossatto, Liana O. Anderson, Tomaz Longhi Santos, David M. J. S. Bowman, Marisol Toledo, Deborah A. Clark, Foster Brown, Michael S. Ross, Williamar Rodrigues Silva, Sintia Valerio Kohler, Carolina V. Castilho, Camille Couralet, Paulo Henrique da Silva Mauricio, Fidel A. Roig, Robert Gliniars, Clément Stahl, Michael D. Swaine, César Augusto Guimarães Finger, Danilo Boanerges Souza, Yadvinder Malhi, Márcia C. M. Marques, Flávia R. C. Costa, Brett P. Murphy, Giselda Durigan, Lip Khoon Kho, Rubens Koloski Chagas, Augusto C. Franco, João Lima Freitas Júnior, Milton Eugene Lieberman, Fabien Wagner, Achim Bräuning, E. N. Chidumayo, Lucy Rowland, Hooz Angela Mendivelso, Fernanda C. G. Cardoso, Fabrício Alvim Carvalho, James Grogan, Oliver Dünisch, Paulo Cesar Botosso, Plínio Barbosa de Camargo, Vivien Rossi, Lynda D. Prior, Timothy R. Baker, José Julio de Toledo, Eduardo Eijji Maeda, Susanne Spannl, Anthony D. Griffiths, Jérôme Chave, Diana Lieberman, José P. Lemos-Filho, Hans Beeckman, Franklin Galvão, Luciano de Souza Arruda, Juan Ignacio Valdez Hernández, Gabriel Sebastian Becker, Simone Aparecida Vieira, Marcos Silveira, Magda Lea Bolzan Zanon, Naoki Okada, Helmut Dalitz, Edilson Consuelo de Oliveira, Mario Tomazello Filho, Paulo Maurício Lima de Alencastro Graça, David M. Drew, José Luis López Ayala, Jennifer Koenig, Elisha Elifuraha, Vivian R. B. Maria, Mark Schulze, Diogo Selhorst, Renato Francisco Rodrigues Marques, Marcio Fedele, Hector Maza Maza Chamba, Lawrence Mbwambo, Luiz E. O. C. Aragão, Maria Raquel Kanieski, Afonso Figueiredo Filho, Julia Krepkowski, J. Julio Camarero, Jan Verbesselt, Karina Melgaço, Jaçanan Eloisa de Freitas Milani, Ligia Ferreira Fedele, Instituto Nacional de Pesquisas Espaciais (INPE), Ministério da Ciência, Tecnologia e Inovação, Ecologie des forêts de Guyane (UMR ECOFOG), Université des Antilles (UA)-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-AgroParisTech-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA), Ecologie et Ecophysiologie Forestières [devient SILVA en 2018] (EEF), Institut National de la Recherche Agronomique (INRA)-Université de Lorraine (UL), School of Geography [Leeds], University of Leeds, Universität Hohenheim, Royal Museum for Central Africa [Tervuren] (RMCA), Instituto Nacional de Pesquisas da Amazônia (INPA), Brazilian Agricultural Research Corporation (Embrapa), University of Tasmania [Hobart, Australia] (UTAS), Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Wageningen University and Research [Wageningen] (WUR), Universidade Federal do Acre (UFAC), Centro de Investigaciones Biológicas (CSIC), Consejo Superior de Investigaciones Científicas [Madrid] (CSIC), Universidade de São Paulo (USP), Universidade Federal do Parana [Curitiba] (UFPR), Universidade Federal do Paraná (UFPR), Universidade Federal de Juiz de Fora (UFJF), Evolution et Diversité Biologique (EDB), Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Biological Sciences Department, University of Zambia, University of Missouri [St. Louis], University of Missouri System, Universidade Federal de Vicosa (UFV), Prefeitura municipal RIo Branco, Patrimoines Locaux et Gouvernance (PALOC), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD), Stellenbosch University, Meisterschule Ebern Schreinerhandwerk, Floresta Estadual Assis, Tanzania Forestry Research Institute (TAFORI), Centro Universitário Estadual da Zona Oeste [Rio de Janeiro] (UEZO), Universidade Federal de Santa Maria = Federal University of Santa Maria [Santa Maria, RS, Brazil] (UFSM), Universidade de Brasilia [Brasília] (UnB), World Agroforestry Center [CGIAR, Kenya] (ICRAF), Consultative Group on International Agricultural Research [CGIAR] (CGIAR), Northern Territory Government, Mount Holyoke College, Illinois State University, Georg-August-University [Göttingen], Universidade Federal de Santa Catarina = Federal University of Santa Catarina [Florianópolis] (UFSC), Malaysian Palm Oil Board, Charles Darwin University, Federal University of Minas Gerais (UFMG), California State University [Monterey Bay] (CSUMB), Campus de Montecillo, Colegio de Postgraduados (CP), University of Helsinki, School of Geography and the Environment [Oxford] (SoGE), University of Oxford [Oxford], Universidad Técnica Particular de Loja (UTPL), USDA Agricultural Research Service [Maricopa, AZ] (USDA), United States Department of Agriculture (USDA), Department of Biological Sciences [Miami], Florida International University [Miami] (FIU), Graduate School of Agriculture, Kyoto University [Kyoto], Botanique et Modélisation de l'Architecture des Plantes et des Végétations (UMR AMAP), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Recherche Agronomique (INRA)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut de Recherche pour le Développement (IRD [France-Sud]), Consejo Nacional de Investigaciones Científicas y Técnicas [Buenos Aires] (CONICET), Department of Earth and Environment, Universidade Paulista [São Paulo] (UNIP), Forêts et Sociétés (UPR Forêts et Sociétés), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), School of Geosciences [Edinburgh], University of Edinburgh, CarboForExpert, Oregon State University (OSU), Ibama, Universidade Federal de Roraima, School of Biological Sciences, University of Aberdeen, Federal University of Amapà, Empresa Brasileira de Pesquisa Agropecuária (Embrapa), Ministério da Agricultura, Pecuária e Abastecimento [Brasil] (MAPA), Governo do Brasil-Governo do Brasil, Universidad Autonoma Gabriel René Moreno (UAGRM), Department of Forest Vegetation, Forestry and Forest Products Research Institute (FFPRI), University of Campinas [Campinas] (UNICAMP), Departamento de Engenharia Florestal, Universidade Federal de Santa Maria (UFSM), TreeD lab - Terrestrial Ecosystem Dynamics, Department of Geosciences and Geography, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-AgroParisTech-Université de Guyane (UG)-Centre National de la Recherche Scientifique (CNRS)-Université des Antilles (UA), University of Tasmania (UTAS), Friedrich Alexander University [Erlangen-Nürnberg], Wageningen University and Research Centre [Wageningen] (WUR), Consejo Superior de Investigaciones Científicas [Spain] (CSIC), Institut de Recherche pour le Développement (IRD)-Université Toulouse III - Paul Sabatier (UT3), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées-Centre National de la Recherche Scientifique (CNRS), World Agroforestry Centre (ICRAF), Georg-August-Universität Göttingen, Federal University of Santa Catarina (UFSC), Colegio de Postgraduados, School of Geography and the Environment [Oxford], United States Department of Agriculture - USDA (USA), Florida International University (FIU), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS)-Institut de Recherche pour le Développement (IRD [France-Sud]), Forêts et Sociétés (Cirad-Es-UPR 105 Forêts et Sociétés), Département Environnements et Sociétés (Cirad-ES), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad), Empresa Brasileira de Pesquisa Agropecuária, São José Dos Campos, UMR Ecologie des Forêts de Guyane, UMR EEF 1137, University of Antwerp, University of Hohenheim, Royal Museum for Central Africa, Instituto Nacional de Pesquisas da Amazônia, Empresa Brasileira de Pesquisa Agropecuária (EMBRAPA), University of Tasmania, University of Erlangen-Nuremberg, Wageningen University, Universidade Federal Do Acre, Consejo Superior de Investigaciones Cientificas (IPE-CSIC), Santa Cruz de la Sierra, CNRS and UniversitéPaul Sabatier, University of Missouri-St. Louis, Universidade Federal de Viçosa (UFV), Prefeitura Municipal de Rio Branco, UMR 208 Patrimoines Locaux et Gouvernance, University of Stellenbosch, Meisterschule Ebern für das Schreinerhandwerk, Floresta Estadual de Assis, Universidade Estadual Do Centro-Oeste, Universidade Federal de Santa Maria, Universidade de Brasília (UnB), Stanford University, University of Illinois at Urbana Champaign, University of Göttingen, Universidade Do Estado de Santa Catarina - UDESC, Universidade Federal de Minas Gerais (UFMG), California State University Monterey Bay, Universidade Federal de Sergipe (UFS), University of Oxford, Universidad Nacional de Loja, USDA Forest Service, Florida International University, Kyoto University, Institut Français de Pondicherry, IRD, Glaciología y Ciencias Ambientales - CONICET, Universidade Estadual Paulista (Unesp), CIRAD, Oregon State University, Universidade Federal de Roraima - UFRR, Universidade Federal Do Amapá, Universidad Autonoma Gabriel René Moreno, Universidade Estadual de Campinas (UNICAMP), Georg-August-Universit, University of Exeter, FABIEN H. WAGNER, Remote Sensing Division, National Institute for Space Research - INPE, LUCIANO DE SOUZA ARRUDA, Prefeitura Municipal de Rio Branco, JEAN-LOUIS DEVINEAU, Département Hommes, Natures, Sociétés, Centre National de la Recherche Scientifique (CNRS) et UMR 208 Patrimoines Locaux et Gouvernance, DAVID M. DREW, Dept. Forest and Wood Science, University of Stellenbosch, OLIVER DÜNISCH, Meisterschule Ebern für das Schreinerhandwerk, GISELDA DURIGAN, Floresta Estadual de Assis, ELISHA ELIFURAHA, Tanzania Forestry Research Institute (TAFORI), MARCIO FEDELE, Departamento de Ciências Florestais, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, LIGIA FERREIRA FEDELE, Departamento de Ciências Florestais, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, AFONSO FIGUEIREDO FILHO, Departamento de Engenharia Florestal – DEF, Universidade Estadual do Centro-Oeste, CÉSAR AUGUSTO GUIMARÃES FINGER, Departamento de Ciências Florestais, Centro de Ciências Rurais, Universidade Federal de Santa Maria, AUGUSTO CÉSAR FRANCO, Departamento de Botânica, Laboratório de Fisiologia Vegetal, Universidade de Brasília, JOÃO LIMA FREITAS JÚNIOR, Programa de Pós-Graduação Ecologia e Manejo de Recursos Naturais, Universidade Federal do Acre, FRANKLIN GALVÃO, Departamento de Engenharia Florestal, Universidade Federal do Paraná, ASTER GEBREKIRSTOS, World Agroforestry Centre (ICRAF), ROBERT GLINIARS, Institute of Botany, University of Hohenheim, PAULO MAURÍCIO LIMA DE ALENCASTRO, Coordenação de Pesquisa em Ecologia, Instituto Nacional de Pesquisas da Amazônia, ANTHONY D. GRIFFITHS, Research Institute for Environment and Livelihoods, Charles Darwin University, JAMES GROGAN, Department of Biological Sciences, Mount Holyoke College, KAIYU GUAN, Department of Earth System Science, Stanford University, JÜRGEN HOMEIER, Department of Plant Ecology, Albrecht von Haller Institute of Plant Sciences, University of Göttingen, MARIA RAQUEL KANIESKI, Departamento de Engenharia Florestal, Universidade do Estado de Santa Catarina, LIP KHOON KHO, Tropical Peat Research Institute, Biological Research Division, Malaysian Palm Oil Board, JENNIFER KOENIG, Research Institute for Environment and Livelihoods, Charles Darwin University, JULIA KREPKOWSKI, Institute of Geography, University of Erlangen-Nuremberg, JOSÉ PIRES LEMOS-FILHO, Departamento de Botânica, Instituto de Ciências Biologicas, Universidade Federal de Minas Gerais, DIANA LIEBERMAN, Division of Science & Environmental Policy, California State University Monterey Bay, MILTON EUGENE LIEBERMAN, Division of Science & Environmental Policy, California State University Monterey Bay, CLAUDIO SERGIO LISI, Departamento de Ciências Florestais, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, TOMAZ LONGHI SANTOS, Departamento de Engenharia Florestal, Universidade Federal do Paraná, JOSÉ LUIS LÓPEZ AYALA, Programa Forestal, Colegio de Postgraduados, EDUARDO EIJJI MAEDA, Department of Geosciences and Geography, University of Helsinki, YADVINDER MALHI, School of Geography and the Environment, University of Oxford, VIVIAN R.B. MARIA, Departamento de Ciências Florestais, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, MARCIA C. M. MARQUES, Departamento de Botânica, Universidade Federal do Paraná, RENATO MARQUES, Departamento de Solos e Engenharia Agrícola, Universidade Federal do Paraná, HECTOR MAZA CHAMBA, Laboratoria de Dendrochronologia y Anatomia de Maderas Espinoza, LAWRENCE MBWAMBO, Tanzania Forestry Research Institute (TAFORI), KARINA LIANA LISBOA MELGAÇO, Coordenação de Pesquisas em Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, HOOZ ANGELA MENDIVELSO, Instituto Pirenaico de Ecologia, Consejo Superior de Investigaciones Cientificas (IPE-CSIC), BRETT P. MURPHY, Research Institute for the Environment and Livelihoods, Charles Darwin University, JOSEPH J. O’BRIEN, Center for Forest Disturbance Science, USDA Forest Service, STEVEN F. OBERBAUER, Department of Biological Sciences, Florida International University, NAOKI OKADA, Graduate School of Agriculture, Kyoto University, RAPHAËL PÉLISSIER, Institut Français de Pondicherry, LYNDA D. PRIOR, School of Biological Sciences, University of Tasmania, FIDEL ALEJANDRO ROIG, Tree Ring and Environmental History Laboratory, Instituto Argentino de Nivología, Glaciología y Ciencias Ambientales – CONICET, MICHAEL ROSS, Department of Earth and Environment, Southeast Environmental Research Center, Florida International University, DAVI RODRIGO ROSSATTO, Departamento de Biologia Aplicada, FCAV, Universidade Estadual Paulista, UNESP, VIVIEN ROSSI, UR B&SEF (Biens et services des écosystèmes forestiers tropicaux), CIRAD, LUCY ROWLAND, School of Geosciences, University of Edinburgh, ERVAN RUTISHAUSER, CarboForExpert, HELLEN SANTANA, Coordenação de Pesquisas em Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, MARK SCHULZE, HJ Andrews Experimental Forest, Oregon State University, DIOGO SELHORST, Ibama, WILLIAMAR RODRIGUES SILVA, PRONAT – Programa de Pós-Graduação em Recurso Naturais, Universidade Federal de Roraima, MARCOS SILVEIRA, Centro de Ciências Biológicas e da Natureza, Laboratóio de Botânica e Ecologia Vegetal, Universidade Federal do Acre, SUSANNE SPANNL, Institute of Geography, University of Erlangen-Nuremberg, MICHAEL D. SWAINE, School of Biological Sciences, University of Aberdeen, JOSÉ JULIO TOLEDO, Departamento de Ciências Ambientais, Universidade Federal do Amapá, MARCOS MIRANDA TOLEDO, CPACP, MARISOL TOLEDO, Instituto Boliviano de Investigacion Forestal (IBIF), Universidad Autonoma Gabriel René Moreno, TAKESHI TOMA, Department of Forest Vegetation, Forestry and Forest Products Research Institute (FFPRI), MARIO TOMAZELLO FILHO, Departamento de Ciências Florestais, Universidade de São Paulo, Escola Superior de Agricultura Luiz de Queiroz, JUAN IGNACIO VALDEZ HERNÁNDEZ, Programa Forestal, Colegio de Postgraduados, JAN VERBESSELT, Laboratory of Geo-information Science and Remote Sensing, Wageningen University, SIMONE APARECIDA VIEIRA, Núcleo de Estudos e Pesquisas Ambientais (NEPAM), Universidade Estadual de Campinas (UNICAMP), GRÉGOIRE VINCENT, UMR AMAP (botAnique et bioinforMatique de l’Architecture des Plantes), IRD, CAROLINA VOLKMER DE CASTILHO, CNPS, FRANZISKA VOLLAND, Institute of Geography, University of Erlangen-Nuremberg, MARTIN WORBES, Crop Production Systems in the Tropics, Georg-August-University, MAGDA LEA BOLZAN ZANON, Departamento de Engenharia Florestal, Centro de Educação Superior Norte, Universidade Federal de Santa Maria, LUIZ E.O.C. ARAGÃO, College of Life and Environmental Sciences, University of Exetex., BRUNO HÉRAULT, CIRAD, UMR Ecologie des Forêts de Guyane, DAMIEN BONAL, INRA, CLÉMENT STAHL, Department of Biology, University of Antwerp, LIANA O. ANDERSON, National Center for Monitoring and Early Warning of Natural Disasters, TIMOTHY R. BAKER, School of Geography, University of Leeds, GABRIEL SEBASTIAN BECKER, Institute of Botany, University of Hohenheim, HANS BEECKMAN, Laboratory for Wood Biology and Xylarium, Royal Museum for Central Africa, DANILO BOANERGES SOUZA, Programa de Pós-graduação em Ciências de Florestas Tropicais, Instituto Nacional de Pesquisas da Amazônia, PAULO CESAR BOTOSSO, CNPF, DAVID M. J. S. BOWMAN, School of Biological Sciences, University of Tasmania, ACHIM BRÄUNING, Institute of Geography, University of Erlangen-Nuremberg, BENJAMIN BREDE, Laboratory of Geo-information Science and Remote Sensing, Wageningen University, FOSTER IRVING BROWN, Centro de Ciências Biológicas e da Natureza, Laboratório de Botânica e Ecologia Vegetal, JESUS JULIO CAMARERO, Instituto Pirenaico de Ecologia, Consejo Superior de Investigaciones Cientificas, PLÍNIO BARBOSA CAMARGO, Centro de Energia Nuclear na Agricultura, Laboratório de Ecologia Isotópica, Universidade de São Paulo, FERNANDA C.G. CARDOSO, Departamento de Botânica, Universidade Federal do Paraná, FABRÍCIO ALVIM CARVALHO, Departamento de Botânica, Universidade Federal de Juiz de Fora (UFJF), WENDESON CASTRO, Programa de Pós-Graduação Ecologia e Manejo de Recursos Naturais, Universidade Federal do Acre, RUBENS KOLOSKI CHAGAS, Departamento de Ecologia do Instituto de Biociências, Universidade de São Paulo (USP), JÉROME CHAVE, UMR 5174 Laboratoire Evolution et DiversitéBiologique, CNRS & UniversitéPaul Sabatier, Toulouse, EMMANUEL N. CHIDUMAYO, Biological Sciences Department, University of Zambia, DEBORAH A. CLARK, Department of Biology, University of Missouri-St. Louis, FLAVIA REGINA CAPELLOTTO COSTA, Coordenação de Pesquisas em Biodiversidade, Instituto Nacional de Pesquisas da Amazônia, CAMILLE COURALET, Laboratory for Wood Biology and Xylarium, Royal Museum for Central Africa, PAULO HENRIQUE DA SILVA, Centro de Ciências Biológicas e da Natureza, Laboratóio de Botânica e Ecologia Vegetal, HELMUT DALITZ, Institute of Botany, University of Hohenheim, VINICIUS RESENDE DE CASTRO, Departamento de Engenharia Florestal, Universidade Federal de Viçosa, JAÇANAN ELOISA DE FREITAS MILANI, Departamento de Engenharia Florestal, Universidade Federal do Paraná, EDILSON CONSUELO DE OLIVEIRA, Centro de Ciêcias Biológicas e da Natureza, Laboratório de Botânica e Ecologia Vegetal, Universidade Federal do Acre, Universidade de São Paulo = University of São Paulo (USP), Université de Toulouse (UT)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Universidade Federal de Viçosa = Federal University of Viçosa (UFV), Georg-August-University = Georg-August-Universität Göttingen, Charles Darwin University [Australia], Universidade Federal de Minas Gerais = Federal University of Minas Gerais [Belo Horizonte, Brazil] (UFMG), Helsingin yliopisto = Helsingfors universitet = University of Helsinki, Universidade Federal de Roraima (UFRR), and Universidade Estadual de Campinas = University of Campinas (UNICAMP)
Subjects: 0106 biological sciences, Canopy, Rainfall, Precipitation (climatology), 010504 meteorology & atmospheric sciences, Tropical forests, Mudança Climática, lcsh:Life, évapotranspiration, forêt tropicale, 01 natural sciences, cycle saisonnier, Madeira, Laboratory of Geo-information Science and Remote Sensing, [SDV.SA.SF]Life Sciences [q-bio]/Agricultural sciences/Silviculture, forestry, assimilation photosynthétique, Climate change, Photosynthesis, Climate, leaf carbon assimilation, wood productivity, tropical forest, Evapotranspiration, Ecology, litière forestière, Physics, lcsh:QE1-996.5, Carbon sink, facteur climatique, Plant litter, PE&RC, Wood, productivité primaire, Chemistry, Productivity (ecology), Terrestrial ecosystem, Carbon assimilation, cycle du carbone, Litterfall, 010603 evolutionary biology, Carbon cycle, Carbon Cycle, Experimental Study, [SDV.EE.ECO]Life Sciences [q-bio]/Ecology, environment/Ecosystems, lcsh:QH540-549.5, Carbono, Tropical Forest, Life Science, Laboratorium voor Geo-informatiekunde en Remote Sensing, Biology, Ecology, Evolution, Behavior and Systematics, 1172 Environmental sciences, Floresta tropical, 0105 earth and related environmental sciences, Earth-Surface Processes, Data Set, précipitation, Carbon sinks, Seasonality, 15. Life on land, Seasonal Variation, Photosynthetic capacity, lcsh:Geology, lcsh:QH501-531, variation saisonnière, Leaf Litter, 13. Climate action, Environmental science, lcsh:Ecology
Abstract: Made available in DSpace on 2018-12-11T17:02:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-04-28 The seasonal climate drivers of the carbon cycle in tropical forests remain poorly known, although these forests account for more carbon assimilation and storage than any other terrestrial ecosystem. Based on a unique combination of seasonal pan-tropical data sets from 89 experimental sites (68 include aboveground wood productivity measurements and 35 litter productivity measurements), their associated canopy photosynthetic capacity (enhanced vegetation index, EVI) and climate, we ask how carbon assimilation and aboveground allocation are related to climate seasonality in tropical forests and how they interact in the seasonal carbon cycle. We found that canopy photosynthetic capacity seasonality responds positively to precipitation when rainfall is < 2000ĝ€-mmĝ€-yrĝ'1 (water-limited forests) and to radiation otherwise (light-limited forests). On the other hand, independent of climate limitations, wood productivity and litterfall are driven by seasonal variation in precipitation and evapotranspiration, respectively. Consequently, light-limited forests present an asynchronism between canopy photosynthetic capacity and wood productivity. First-order control by precipitation likely indicates a decrease in tropical forest productivity in a drier climate in water-limited forest, and in current light-limited forest with future rainfall < 2000ĝ€-mmĝ€-yrĝ'1. Author(s) 2016. Remote Sensing Division National Institute for Space Research - INPE São José Dos Campos CIRAD UMR Ecologie des Forêts de Guyane INRA UMR EEF 1137 INRA UMR Ecologie des Forêts de Guyane Department of Biology University of Antwerp National Center for Monitoring and Early Warning of Natural Disasters - CEMADEN São José Dos Campos School of Geography University of Leeds Institute of Botany University of Hohenheim Laboratory for Wood Biology and Xylarium Royal Museum for Central Africa Programa de Pós-graduação em Ciências de Florestas Tropicais Instituto Nacional de Pesquisas da Amazônia Embrapa Florestas Brazilian Agricultural Research Corporation School of Biological Sciences University of Tasmania Institute of Geography University of Erlangen-Nuremberg Laboratory of Geo-information Science and Remote Sensing Wageningen University Centro de Ciências Biológicas e da Natureza Laboratóio de Botânica e Ecologia Vegetal Universidade Federal Do Acre Instituto Pirenaico de Ecologia Consejo Superior de Investigaciones Cientificas (IPE-CSIC) Instituto Boliviano de Investigacion Forestal (IBIF) Santa Cruz de la Sierra Centro de Energia Nuclear Na Agricultura Laboratóio de Ecologia Isotópica Universidade de SÃo Paulo Departamento de Botânica Universidade Federal Do Paraná Departamento de Botânica Universidade Federal de Juiz de Fora (UFJF) Programa de Pós-Graduação Ecologia e Manejo de Recursos Naturais Universidade Federal Do Acre Departamento de Ecologia Do Instituto de Biociências Universidade de São Paulo (USP) UMR 5174 Laboratoire Evolution et DiversitéBiologique CNRS and UniversitéPaul Sabatier Biological Sciences Department University of Zambia Department of Biology University of Missouri-St. Louis Coordenação de Pesquisas em Biodiversidade Instituto Nacional de Pesquisas da Amazônia Departamento de Engenharia Florestal Universidade Federal de Viçosa (UFV) Departamento de Engenharia Florestal Universidade Federal Do Paraná Centro de Ciêcias Biológicas e da Natureza Laboratório de Botânica e Ecologia Vegetal Universidade Federal Do Acre Prefeitura Municipal de Rio Branco Département Hommes Natures Sociétés Centre National de la Recherche Scientifique (CNRS) et UMR 208 Patrimoines Locaux et Gouvernance, Cedex 05 Dept. Forest and Wood Science University of Stellenbosch Meisterschule Ebern für das Schreinerhandwerk Floresta Estadual de Assis Tanzania Forestry Research Institute (TAFORI), P.O. Box Departamento de Ciências Florestais Universidade de São Paulo Escola Superior de Agricultura Luiz de Queiroz Departamento de Engenharia Florestal - DEF Universidade Estadual Do Centro-Oeste Departamento de Ciências Florestais Centro de Ciências Rurais Universidade Federal de Santa Maria Departamento de Botânica Laboratório de Fisiologia Vegeta Universidade de Brasília Instituto de Ciências Biológicas World Agroforestry Centre (ICRAF), P.O. Box Coordenação de Pesquisa em Ecologia Instituto Nacional de Pesquisas da Amazônia, C.P. 478 Departement of Land Resource Management Northern Territory Government Research Institute for Environment and Livelihoods Charles Darwin University Department of Biological Sciences Mount Holyoke College Department of Earth System Science Stanford University Department of Natural Resources and Environmental Sciences University of Illinois at Urbana Champaign Department of Plant Ecology Albrecht von Haller Institute of Plant Sciences University of Göttingen Departamento de Engenharia Florestal Universidade Do Estado de Santa Catarina - UDESC Tropical Peat Research Institute Biological Research Division Malaysian Palm Oil Board Departamento de Botânica Instituto de Ciências Biologicas Universidade Federal de Minas Gerais Division of Science and Environmental Policy California State University Monterey Bay Departamento de Biologia Universidade Federal de Sergipe Programa Forestal Colegio de Postgraduados Department of Geosciences and Geography University of Helsinki School of Geography and the Environment University of Oxford Departamento de Solos e Engenharia Agrícola Universidade Federal Do Paraná Laboratoria de Dendrochronologia y Anatomia de Maderas Espinoza Universidad Nacional de Loja Research Institute for the Environment and Livelihoods Charles Darwin University Center for Forest Disturbance Science USDA Forest Service Department of Biological Sciences Florida International University Graduate School of Agriculture Kyoto University Institut Français de Pondicherry UMR AMAP (BotAnique et BioinforMatique de l'Architecture des Plantes) IRD Tree Ring and Environmental History Laboratory Instituto Argentino de Nivología Glaciología y Ciencias Ambientales - CONICET Department of Earth and Environment Southeast Environmental Research Center Florida International University Departamento de Biologia Aplicada FCAV Universidade Estadual Paulista UNESP UR BandSEF (Biens et Services des Écosystèmes Forestiers Tropicaux) CIRAD School of Geosciences University of Edinburgh CarboForExpert HJ Andrews Experimental Forest Oregon State University Ibama PRONAT - Programa de Pós-Graduação em Recurso Naturais Universidade Federal de Roraima - UFRR School of Biological Sciences University of Aberdeen Departamento de Ciências Ambientais Universidade Federal Do Amapá Embrapa Cocais Brazilian Agricultural Research Corporation Instituto Boliviano de Investigacion Forestal (IBIF) Universidad Autonoma Gabriel René Moreno Department of Forest Vegetation Forestry and Forest Products Research Institute (FFPRI) Núcleo de Estudos e Pesquisas Ambientais (NEPAM) Universidade Estadual de Campinas (UNICAMP) Embrapa Roraima Brazilian Agricultural Research Corporation Crop Production Systems in the Tropics Georg-August-Universit Departamento de Engenharia Florestal Centro de Educação Superior Norte Universidade Federal de Santa Maria College of Life and Environmental Sciences University of Exeter Departamento de Biologia Aplicada FCAV Universidade Estadual Paulista UNESP
Published: 2016
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19. Comprehensive Profiling of Ethylene Response Factor Expression Identifies Ripening-Associated ERF Genes and Their Link to Key Regulators of Fruit Ripening in Tomato

Author: Elie Maza, Isabelle Mila, Jean-Paul Roustan, Mohamed Zouine, Mingchun Liu, Julien Pirrello, Pierre Frasse, Eduardo Purgatto, Mondher Bouzayen, Lázaro Eustáquio Pereira Peres, Bruna Lima Gomes, Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Sichuan University - SCU (CHINA), Universidade de São Paulo - USP (BRAZIL), Génomique et Biotechnologie des Fruits - GBF (Castanet-Tolosan, France), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Sichuan University, Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-Ecole Nationale Supérieure Agronomique de Toulouse-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Universidade de São Paulo (USP), Liu, Mingchun, Pirrello, Julien, Sichuan University [Chengdu] (SCU), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Laboratoire d'Excellence entitled TULIP [ANR-10-LABX-41], European COST Action [FA1106], and CAPES-COFECUB program [Sv 815-14]
Subjects: 0106 biological sciences, 0301 basic medicine, Agronomie, Physiology, [SDV.SA.AGRO]Life Sciences [q-bio]/Agricultural sciences/Agronomy, Hormone ethylene, Plant Science, Genes, Plant, Response Elements, 01 natural sciences, Climacteric fruit, [SDV.GEN.GPL]Life Sciences [q-bio]/Genetics/Plants genetics, 03 medical and health sciences, Solanum lycopersicum, Plant Growth Regulators, Gene Expression Regulation, Plant, Gene expression, Génétique des plantes, Genes, Regulator, Genetics, Cluster Analysis, Gene Regulatory Networks, Promoter Regions, Genetic, Gene, Transcription factor, Plant Proteins, Regulation of gene expression, biology, Reverse Transcriptase Polymerase Chain Reaction, Gene Expression Profiling, fungi, technology, industry, and agriculture, food and beverages, Ripening, Articles, Ethylenes, biology.organism_classification, Cell biology, Gene expression profiling, 030104 developmental biology, Biochemistry, Fruit, gcc box, transcriptional regulation, carotenoaccumulation, signal-transduction, functional-analysis, repressor version, Mutation, Solanum, Climacteric, 010606 plant biology & botany
Abstract: International audience; Our knowledge of the factors mediating ethylene-dependent ripening of climacteric fruit remains limited. The transcription of ethylene-regulated genes is mediated by ethylene response factors (ERFs), but mutants providing information on the specific role of the ERFs in fruit ripening are still lacking, likely due to functional redundancy among this large multigene family of transcription factors. We present here a comprehensive expression profiling of tomato (Solanum lycopersicum) ERFs in wild-type and tomato ripening-impaired tomato mutants (Never-ripe [Nr], ripening-inhibitor [rin], and non-ripening [nor]), indicating that out of the 77 ERFs present in the tomato genome, 27 show enhanced expression at the onset of ripening while 28 display a ripeningassociated decrease in expression, suggesting that different ERFs may have contrasting roles in fruit ripening. Among the 19 ERFs exhibiting the most consistent up-regulation during ripening, the expression of 11 ERFs is strongly down-regulated in rin, nor, and Nr tomato ripening mutants, while only three are consistently up-regulated. Members of subclass E, SlERF.E1, SlERF.E2, and SlERF.E4, show dramatic down-regulation in the ripening mutants, suggesting that their expression might be instrumental in fruit ripening. This study illustrates the high complexity of the regulatory network connecting RIN and ERFs and identifies subclass E members as the most active ERFs in ethylene- and RIN/NOR-dependent ripening.
Published: 2015
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20. Effect of curcumin-encapsulated Pluronic® F-127 over duo-species biofilm of Streptococcus mutans and Candida albicans

Author: Diego Dantas Lopes dos Santos, João Felipe Besegato, Marlus Chorilli, João Augusto Oshiro Junior, Vanderlei Salvador Bagnato, Dongmei Deng, Priscila Borges Gobbo de Melo, Alessandra Nara de Souza Rastelli, Universidade Estadual Paulista (UNESP), State University of Paraíba-UEPB, University of Amsterdam and Vrije Universiteit Amsterdam, Universidade de São Paulo (USP), and Preventive Dentistry
Subjects: Curcumin, medicine.medical_treatment, Photodynamic therapy, Dermatology, Streptococcus mutans, Minimum inhibitory concentration, Candida albicans, medicine, Photosensitizer, Micelles, biology, Chemistry, Biofilm, Antimicrobial, biology.organism_classification, Molecular biology, Nystatin, DIAGNÓSTICO, Photochemotherapy, Biofilms, Surgery, SDG 6 - Clean Water and Sanitation, medicine.drug
Abstract: Made available in DSpace on 2022-04-29T08:35:30Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-04-01 To assess the effect of curcumin-encapsulated Pluronic® F-127 (Cur-Plu) during antimicrobial photodynamic therapy (aPDT) over duo-species biofilm of Streptococcus mutans and Candida albicans. Thermal analysis, optical absorption, and fluorescence spectroscopy were evaluated. Minimum inhibitory concentration (MIC) and minimum bactericidal/fungal concentration were obtained. The biofilms were cultured for 48 h at 37 °C and treated according to the groups: P + M + L + (photosensitizer encapsulated with Pluronic® F-127 + light); P + D + L + (photosensitizer incorporated in 1% DMSO + light); P − M + L + (no Pluronic® F-127 + light); P − D + L + (1% DMSO + light); P − L + (Milli-Q water + light); P + M + L − (photosensitizer encapsulated with Pluronic® F-127 no light); P + D + L − (photosensitizer in 1% DMSO, no light); P − M + L − (Pluronic® F-127 no light); P − D + L − (1% DMSO, no light); P − L − (Milli-Q water, no light; negative control group); CHX (0.2% chlorhexidine, positive control group); and NYS (Nystatin). Dark incubation of 5 min was used. The groups that received aPDT were irradiated by blue LED (460 nm, 15 J/cm2). Cell viability of the biofilms was performed by colony-forming units (CFU/mL) and confocal microscopy. Two-way ANOVA followed by Tukey’s post hoc test was used at a significance level of 5%. P + D + L + and P + M + L + groups exhibited better log-reduction for both Candida albicans and Streptococcus mutans biofilms than P − M + L + , P − L + , and P − D + L + experimental groups. Furthermore, P + M + L + and P + D + L + showed greater reduction for Candida albicans than for Streptococcus mutans. aPDT mediated by Cur-Plu can be a potential strategy for biofilm control against duo-species biofilm of Streptococcus mutans and Candida albicans. Department of Dental Materials and Prosthodontics School of Dentistry Araraquara São Paulo State University – UNESP, São Paulo Department of Restorative Dentistry School of Dentistry Araraquara São Paulo State University – UNESP, 1680 Humaitá St., MailBox: 331, São Paulo Department of Drugs and Medicines School of Pharmaceutical Sciences Araraquara São Paulo State University – UNESP, São Paulo Graduate Program in Pharceutical Sciences State University of Paraíba-UEPB Department of Preventive Dentistry Academic Centre for Dentistry Amsterdam – ACTA University of Amsterdam and Vrije Universiteit Amsterdam Department of Physics and Materials Science Physics Institute of São Carlos - IFSC University of São Paulo – USP, São Paulo Department of Dental Materials and Prosthodontics School of Dentistry Araraquara São Paulo State University – UNESP, São Paulo Department of Restorative Dentistry School of Dentistry Araraquara São Paulo State University – UNESP, 1680 Humaitá St., MailBox: 331, São Paulo Department of Drugs and Medicines School of Pharmaceutical Sciences Araraquara São Paulo State University – UNESP, São Paulo
Published: 2022
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21. The nasus gland: A new gland in soldiers of Angularitermes (Termitidae, Nasutitermitinae)

Author: Alain Robert, Christian Bordereau, Eliana M. Cancello, Jan Šobotník, Joice P. Constantini, Thomas Bourguignon, Tiago F. Carrijo, Barbora Křížková, Czech University of Life Science, University of Sydney, Museu de Zoologia (MZ), Universidade de São Paulo (USP), Centre des Sciences du Goût et de l'Alimentation [Dijon] (CSGA), Institut National de la Recherche Agronomique (INRA)-Université de Bourgogne (UB)-AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA), Centre National de la Recherche Scientifique (CNRS), Université de Bourgogne (UB), Projects CIGA No. 20154314 and IGA No. B03/15 (Czech University of Life Sciences, Prague), the São Paulo Research Foundation (FAPESP) (2013/03767-0), and the project grant 2013/20068-9, the National Council for Scientific and Technological Development, Brazil (CNPq) (308227/2013-0 and 562256/2010-5)., Museu de Zoologia ( MZ ), Universidade de São Paulo ( USP ), Centre des Sciences du Goût et de l'Alimentation [Dijon] ( CSGA ), Institut National de la Recherche Agronomique ( INRA ) -Université de Bourgogne ( UB ) -AgroSup Dijon - Institut National Supérieur des Sciences Agronomiques, de l'Alimentation et de l'Environnement-Centre National de la Recherche Scientifique ( CNRS ), Institut National de la Recherche Agronomique ( INRA ), Centre National de la Recherche Scientifique ( CNRS ), and Université de Bourgogne ( UB )
Subjects: defence, Exocrine gland, [ SDV.BA.ZI ] Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Zoology, Biology, [ SDV.BA ] Life Sciences [q-bio]/Animal biology, exocrine organ, Exocrine Glands, Microscopy, Electron, Transmission, Single species, stomatognathic system, biology.animal, medicine, Animals, Angularitermes, Secretion, Ecology, Evolution, Behavior and Systematics, termitoidea, Synapomorphy, Cockroach, cephalic gland, [SDV.BA]Life Sciences [q-bio]/Animal biology, General Medicine, Anatomy, biology.organism_classification, [SDV.BA.ZI]Life Sciences [q-bio]/Animal biology/Invertebrate Zoology, Termitidae, medicine.anatomical_structure, Insect Science, Microscopy, Electron, Scanning, Ultrastructure, isoptera, neotropical region, termite, Developmental Biology
Abstract: Termites have developed many exocrine glands, generally dedicated to defence or communication. Although a few of these glands occur in all termite species, or represent synapomorphies of larger clades, others are morphological innovations of a single species, or a few related species. Here, we describe the nasus gland, a new gland occurring at the base of the nasus of Angularitermes soldiers. The nasus gland is composed of class 1, 2, and 3 secretory cells, a rare combination that is only shared by the sternal and tergal glands of some termites and cockroaches. The ultrastructural observations suggest that the secretion is produced by class 2 and 3 secretory cells, and released mostly by class 3 cells. The base of the nasus has a rough appearance due to numerous pits bearing openings of canals conducting the secretion from class 3 secretory cells to the exterior. We tentatively assign a defensive function to the nasus gland, although further research is needed to confirm this function. Although the gland is described only from species of Angularitermes, other genera of Nasutitermitinae also present a rough nasus base, suggesting the presence of a similar, possibly homologous, gland.
Published: 2015
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22. Plasmodium vivax Diversity and Population Structure across Four Continents

Author: Ivo Mueller, Céline Barnadas, Marcelo U. Ferreira, Arsène Ratsimbasoa, Priscila T. Rodrigues, Didier Menard, Ingrid Felger, Michela Menegon, Jorge Bendezu, Carlo Severini, Pamela Orjuela-Sánchez, Nadira D. Karunaweera, Annette Erhart, Bakri Y. M. Nour, Cristian Koepfli, Peter Van den Eede, Nguyen Van Hong, Tiago Antao, Dionicia Gamboa, The Walter and Eliza Hall Institute of Medical Research (WEHI), Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Liverpool School of Tropical Medicine (LSTM), University of São Paulo (USP), Institute of Tropical Medicine [Antwerp] (ITM), Instituto de Medicina Tropical 'Alexander von Humboldt' (IMT AvH), Universidad Peruana Cayetano Heredia (UPCH), National Institute of Malariology, Parasitology and Entomology [Hanoi], Radicaux Libres, Substrats Énergétiques et Physiopathologie Cérébrale, Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon, Ministère de la Santé , du Planning Familial et de la Protection Sociale Madagascar, Ministère de la Santé, du Planning Familial et de la Protection Sociale, Institut Pasteur du Cambodge, Réseau International des Instituts Pasteur (RIIP), Istituto Superiore di Sanita [Rome], Department of Infectious, Parasitic and Immunomediated Diseases, Istituto Superiore di Sanita` (ISS), Department of Parasitology, Blue Nile National Institute for Communicable Diseases, University of Gezira-University of Gezira, University of Colombo [Sri Lanka], University of Melbourne, and Swiss Tropical Institute
Subjects: MESH: Geography, Plasmodium vivax, Population genetics, MESH: Africa, Geografia mèdica, Linkage Disequilibrium, MESH: Madagascar, MESH: Genotype, Cohort Studies, 0302 clinical medicine, Effective population size, [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases, Cluster Analysis, MESH: Genetic Variation, MESH: Cohort Studies, Genetics, 0303 health sciences, education.field_of_study, Public health, biology, Geography, MESH: Asia, lcsh:Public aspects of medicine, Anopheles, Plasmodium vivax/genetics/isolation & purification, 3. Good health, MESH: Plasmodium vivax, Infectious Diseases, qx_20, MESH: Linkage Disequilibrium, Medical geography, Genetic structure, Malaria, Vivax/epidemiology/parasitology/transmission, purl.org/pe-repo/ocde/ford#3.03.06 [https], Research Article, lcsh:Arctic medicine. Tropical medicine, Asia, Genotype, lcsh:RC955-962, 030231 tropical medicine, Population, Zoology, Microsatellite Repeats/genetics, MESH: Genetics, Population, Malària, 03 medical and health sciences, Genetic variation, Madagascar, Malaria, Vivax, MESH: Americas, Humans, PLASMODIUM, education, Alleles, 030304 developmental biology, Genetic diversity, MESH: Humans, Asia/epidemiology, Madagascar/epidemiology, MESH: Alleles, Public Health, Environmental and Occupational Health, MESH: Malaria, Vivax, Genetic Variation, lcsh:RA1-1270, MESH: Haplotypes, biology.organism_classification, MESH: Cluster Analysis, Salut pública, wc_750, Malaria, Genetics, Population, Africa/epidemiology, Haplotypes, qx_135, Africa, MESH: Microsatellite Repeats, Americas, Americas/epidemiology, Microsatellite Repeats
Abstract: Plasmodium vivax is the geographically most widespread human malaria parasite. To analyze patterns of microsatellite diversity and population structure across countries of different transmission intensity, genotyping data from 11 microsatellite markers was either generated or compiled from 841 isolates from four continents collected in 1999–2008. Diversity was highest in South-East Asia (mean allelic richness 10.0–12.8), intermediate in the South Pacific (8.1–9.9) Madagascar and Sudan (7.9–8.4), and lowest in South America and Central Asia (5.5–7.2). A reduced panel of only 3 markers was sufficient to identify approx. 90% of all haplotypes in South Pacific, African and SE-Asian populations, but only 60–80% in Latin American populations, suggesting that typing of 2–6 markers, depending on the level of endemicity, is sufficient for epidemiological studies. Clustering analysis showed distinct clusters in Peru and Brazil, but little sub-structuring was observed within Africa, SE-Asia or the South Pacific. Isolates from Uzbekistan were exceptional, as a near-clonal parasite population was observed that was clearly separated from all other populations (F ST>0.2). Outside Central Asia F ST values were highest (0.11–0.16) between South American and all other populations, and lowest (0.04–0.07) between populations from South-East Asia and the South Pacific. These comparisons between P. vivax populations from four continents indicated that not only transmission intensity, but also geographical isolation affect diversity and population structure. However, the high effective population size results in slow changes of these parameters. This persistency must be taken into account when assessing the impact of control programs on the genetic structure of parasite populations., Author Summary Plasmodium vivax is the predominant malaria parasite in Latin America, Asia and the South Pacific. Different factors are expected to shape diversity and population structure across continents, e.g. transmission intensity which is much lower in South America as compared to Southeast-Asia and the South Pacific, or geographical isolation of P. vivax populations in the South Pacific. We have compiled data from 841 isolates from South and Central America, Africa, Central Asia, Southeast-Asia and the South Pacific typed with a panel of 11 microsatellite markers. Diversity was highest in Southeast-Asia, where transmission is intermediate-high and migration of infected hosts is high, and lowest in South America and Central Asia where malaria transmission is low and focal. Reducing the panel of microsatellites showed that 2–6 markers are sufficient for genotyping for most drug trials and epidemiological studies, as these markers can identify >90% of all haplotypes. Parasites clustered according to continental origin, with high population differentiation between South American and Central Asian populations and the other populations, and lowest differences between Southeast-Asia and the South Pacific. Current attempts to reduce malaria transmission might change this pattern, but only after transmission is reduced for an extended period of time.
Published: 2014
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23. Pharmacological Evaluation and Preparation of Nonsteroidal Anti-Inflammatory Drugs Containing an N-Acyl Hydrazone Subunit

Author: Gustavo Henrique Goulart Trossini, Maria Elisa Lopes Pires, Man Chin Chung, Priscila Longhin Bosquesi, Rafael Consolin Chelucci, Luiz Antonio Dutra, Karina Pereira Barbieri, Jean Leandro dos Santos, Thais Regina Ferreira de Melo, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), and Universidade Estadual Paulista (Unesp)|Universidade de São Paulo (USP)
Subjects: Male, Models, Molecular, Hydrazone, Pharmacology, Non-steroidal, lcsh:Chemistry, Mice, lcsh:QH301-705.5, Spectroscopy, media_common, anti-inflammatory, chemistry.chemical_classification, non-steroidal anti-inflammatory, Analgesics, Sulfonamides, biology, Chemistry, Anti-Inflammatory Agents, Non-Steroidal, MODELAGEM MOLECULAR, General Medicine, analgesic, NSAID, Computer Science Applications, Molecular Docking Simulation, docking, Gastrointestinal Hemorrhage, medicine.drug, Protein Binding, Drug, medicine.drug_class, Stereochemistry, media_common.quotation_subject, Analgesic, Catalysis, Anti-inflammatory, Article, Inorganic Chemistry, hydrazone, In vivo, molecular hybridization, molecular modeling, COX, parasitic diseases, medicine, Animals, Physical and Theoretical Chemistry, Rats, Wistar, Molecular Biology, Inflammation, Cyclooxygenase 2 Inhibitors, Organic Chemistry, Hydrazones, Rats, lcsh:Biology (General), lcsh:QD1-999, Docking (molecular), Celecoxib, Cyclooxygenase 2, Drug Design, biology.protein, Cyclooxygenase 1, Pyrazoles, Cyclooxygenase
Abstract: Made available in DSpace on 2016-01-28T16:56:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2014. Added 1 bitstream(s) on 2016-01-28T17:02:44Z : No. of bitstreams: 1 ISSN1422-0067-2014-15-04-5821-5837.pdf: 556278 bytes, checksum: b3ccb2d14d850126601995ebdd26a2bb (MD5) A series of anti-inflammatory derivatives containing an N-acyl hydrazone subunit (4a–e) were synthesized and characterized. Docking studies were performed that suggest that compounds 4a–e bind to cyclooxygenase (COX)-1 and COX-2 isoforms, but with higher affinity for COX-2. The compounds display similar anti-inflammatory activities in vivo, although compound 4c is the most effective compound for inhibiting rat paw edema, with a reduction in the extent of inflammation of 35.9% and 52.8% at 2 and 4 h, respectively. The anti-inflammatory activity of N-acyl hydrazone derivatives was inferior to their respective parent drugs, except for compound 4c after 5 h. Ulcerogenic studies revealed that compounds 4a–e are less gastrotoxic than the respective parent drug. Compounds 4b–e demonstrated mucosal damage comparable to celecoxib. The in vivo analgesic activities of the compounds are higher than the respective parent drug for compounds 4a–b and 4d–e. Compound 4a was more active than dipyrone in reducing acetic-acid-induced abdominal constrictions. Our results indicate that compounds 4a–e are anti-inflammatory and analgesic compounds with reduced gastrotoxicity compared to their respective parent non-steroidal anti-inflammatory drugs. Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Fármacos e Médicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Rodovia Araraquara-Jaú Km. 01, Campus Ville, CEP 14801902, SP, Brasil Faculty of Pharmaceutical Science, University of São Paulo, Av. Professor Lineu Prestes 580, São Paulo 05508-900, SP, Brazil Universidade Estadual Paulista Júlio de Mesquita Filho, Departamento de Fármacos e Médicamentos, Faculdade de Ciências Farmacêuticas de Araraquara, Araraquara, Rodovia Araraquara-Jaú Km. 01, Campus Ville, CEP 14801902, SP, Brasil
Published: 2014
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24. Assessment of Response to Lithium Maintenance Treatment in Bipolar Disorder: A Consortium on Lithium Genetics (ConLiGen) Report

Author: J. Raymond DePaulo, Martin Alda, Alfonso Tortorella, Susan G. Leckband, Jean-Michel Aubry, James B. Potash, Clara Brichant-Petitjean, Layla Kassem, Joanna Hauser, Tomas Novak, David Zilles, Claire O'Donovan, Rebecca Hoban, Sarah Kittel-Schneider, Claire Slaney, Lena Backlund, Peter Falkai, Andrew T. A. Cheng, John R. Kelsoe, Andrea Pfennig, Janice M. Fullerton, Marcella Rietschel, Caterina Chillotti, Stéphane Jamain, Elise Bui, Po-Hsiu Kuo, Alessio Squassina, Claudio E. M. Banzato, Joanna M. Biernacka, Lisa R. Seymour, Naomi R. Wray, Ichiro Kusumi, Thomas Stamm, Fernando S. Goes, Audrey Nallet, Sarah K. Tighe, Giovanni Severino, Jean-Pierre Kahn, Maria Neuner, Nirmala Akula, Norio Ozaki, Francis J. McMahon, Frank Bellivier, Andreas Reif, Ryota Hashimoto, Barbara W. Schweizer, Palmiero Monteleone, Florian Seemüller, Urs Heilbronner, Sevilla D. Detera-Wadleigh, Pavla Stopkova, Martin Schalling, Julie Garnham, Sébastien Gard, Mark A. Frye, Eva Z. Reininghaus, Lina Martinsson, Urban Ösby, Maria Grigoroiu-Serbanescu, Sara Richardson, Tadafumi Kato, Marion Leboyer, Catharina Lavebratt, Piotr M. Czerski, Scott R. Clark, Daniela Reich-Erkelenz, Sebastian Kliwicki, Guy A. Rouleau, Oliver Gruber, Gonzalo Laje, Adam Wright, Gustavo Turecki, Roy H. Perlis, Takuya Masui, Mirko Manchia, Bernhard T. Baune, Mazda Adli, Michael Bauer, Sven Cichon, Frank Mondimore, Bruno Etain, Philip B. Mitchell, Thomas G. Schulze, Peter P. Zandi, Raffaella Ardau, Peter R. Schofield, Susanne Bengesser, Liping Hou, Janusz K. Rybakowski, Mario Maj, Clarissa de Rosalmeida Dantas, Louise Frisén, O. Schubert, Carlos Jaramillo, Maria Del Zompo, Paul Grof, Cynthia V. Calkin, Jo Steele, Jordan W. Smoller, Alain Malafosse, Manchia, M, Adli, M, Akula, N, Ardau, R, Aubry, Jm, Backlund, L, Banzato, Ce, Baune, Bt, Bellivier, F, Bengesser, S, Biernacka, Jm, Brichant Petitjean, C, Bui, E, Calkin, Cv, Cheng, At, Chillotti, C, Cichon, S, Clark, S, Czerski, Pm, Dantas, C, Zompo, Md, Depaulo, Jr, Detera Wadleigh, Sd, Etain, B, Falkai, P, Frisén, L, Frye, Ma, Fullerton, J, Gard, S, Garnham, J, Goes, F, Grof, P, Gruber, O, Hashimoto, R, Hauser, J, Heilbronner, U, Hoban, R, Hou, L, Jamain, S, Kahn, Jp, Kassem, L, Kato, T, Kelsoe, Jr, Kittel Schneider, S, Kliwicki, S, Kuo, Ph, Kusumi, I, Laje, G, Lavebratt, C, Leboyer, M, Leckband, Sg, López Jaramillo, Ca, Maj, Mario, Malafosse, A, Martinsson, L, Masui, T, Mitchell, Pb, Mondimore, F, Monteleone, P, Nallet, A, Neuner, M, Novák, T, O'Donovan, C, Osby, U, Ozaki, N, Perlis, Rh, Pfennig, A, Potash, Jb, Reich Erkelenz, D, Reif, A, Reininghaus, E, Richardson, S, Rouleau, Ga, Rybakowski, Jk, Schalling, M, Schofield, Pr, Schubert, Ok, Schweizer, B, Seemüller, F, Grigoroiu Serbanescu, M, Severino, G, Seymour, Lr, Slaney, C, Smoller, Jw, Squassina, A, Stamm, T, Steele, J, Stopkova, P, Tighe, Sk, Tortorella, Alfonso Antonio Vincenzo, Turecki, G, Wray, Nr, Wright, A, Zandi, Pp, Zilles, D, Bauer, M, Rietschel, M, Mcmahon, Fj, Schulze, Tg, Alda, M., Department of Psychiatry, Dalhousie University [Halifax], Department of Psychiatry and Psychotherapy, Charité - UniversitätsMedizin = Charité - University Hospital [Berlin], Human Genetics Branch, National Institutes of Health [Bethesda] (NIH)-National Institute of Mental Health (NIMH), Unit of Clinical Pharmacology, University-Hospital of Cagliari, Department of Mental Health and Psychiatry, University Hospital of Geneva, Trinity College Dublin-St. James's Hospital, University of Campinas [Campinas] (UNICAMP), University of Adelaide, Pôle de Psychiatrie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Medical University Graz, Mayo Clinic, Division of Epidemiology and Genetics, Academia Sinica-Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Department of Genomics, Life and Brain Center, University of Bonn, Psychiatric Genetic Unit, Poznan University of Medical Sciences, Section of Neuroscience and Clinical Pharmacology, University of Cagliari, Department of Psychiatry and Behavioral Sciences, Johns Hopkins University School of Medicine, Institut Mondor de Recherche Biomédicale (IMRB), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12)-IFR10, University of Homburg, Genetics of Mental Illness and Brain Function, Neuroscience Research Australia, Service de psychiatrie adulte, Université Bordeaux Segalen - Bordeaux 2-Hôpital Charles Perrens-Centre Expert Trouble Bipolaire, Mood Disorders Center of Ottawa (MDCO), University of Ottawa [Ottawa], University of Toronto, Georg-August-University [Göttingen], Osaka University Graduate School of Medicine, University of California [San Diego] (UC San Diego), University of California-University of California, Service de Psychiatrie et Psychologie Clinique, Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Hôpital Jeanne-d'Arc, Laboratory for Molecular Dynamics of Mental Disorders, RIKEN Center for Brain Science [Wako] (RIKEN CBS), RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN)-RIKEN - Institute of Physical and Chemical Research [Japon] (RIKEN), Mental Health Sciences Unit, University College of London [London] (UCL), Department of Adult Psychiatry, Institute of Epidemiology and Preventive Medicine, National Taiwan University [Taiwan] (NTU), Hokkaido University Graduate School of Medicine, Molecular Medicine and Surgery department, Karolinska Institutet [Stockholm], Center for Molecular Medicine, Karolinska University Hospital [Stockholm], Department of Pharmacy, Veterans Affairs San Diego Healthcare System, Skaggs School of Pharmacy and Pharmaceutical Sciences [San Diego], University of Antioquia, University of Napoli, School of Psychiatry, University of New South Wales [Sydney] (UNSW)-Black Dog Institute, Prague Psychiatric Center, University of Prague, Fujita Health University School of Medicine, Nagoya University Graduate School of Medicine, Harvard Medical School [Boston] (HMS)-Massachusetts General Hospital [Boston], Technische Universität Dresden = Dresden University of Technology (TU Dresden), University of Iowa [Iowa City], Center of Excellence in Neuroscience, CHU de Montréal, Department of Medicine, Centre de Recherche du Centre Hospitalier de l’Université de Montréal (CR CHUM), Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM)-Centre Hospitalier de l'Université de Montréal (CHUM), Université de Montréal (UdeM)-Université de Montréal (UdeM), Biometric Psychiatric Genetics Research Unit, Alexandru Obregia Psychiatric Hospital, McGill Group for Suicide Studies, Douglas Mental Health University Institute, Queensland Brain Institute, University of Queensland [Brisbane], Department of Mental Health, Johns Hopkins Bloomberg School of Public Health [Baltimore], Johns Hopkins University (JHU)-Johns Hopkins University (JHU), Department of Genetic Epidemiology in Psychiatry [Mannhein], Universität Heidelberg [Heidelberg]-Central Institute of Mental Health Mannheim, The work on assessment of lithium response has been supported by a grant from Canadian Institutes of Health Research #64410 to MA. MG-S was supported by Unitatea Executiva pentru Finantarea Invatamantului Superior, a Cercetarii, Dezvoltarii si Inovarii (UEFISCDI), Bucharest, Romania (grant no. 89/2012). JMA and AN were supported by a grant from the Swiss National Foundation (#32003B_125469/1 to JM Aubry). ConLiGen is in part supported by funds from the Intramural Research Program of the National Institute of Mental Health (NIMH) at the National Institutes of Health (NIH), Department of Health and Human Services, United States Government, Aubry, Jean-Michel, Universidade Estadual de Campinas = University of Campinas (UNICAMP), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), Institut National de la Santé et de la Recherche Médicale (INSERM)-IFR10-Université Paris-Est Créteil Val-de-Marne - Paris 12 (UPEC UP12), Georg-August-University = Georg-August-Universität Göttingen, University of California (UC)-University of California (UC), Guellaen, Georges, Université Bordeaux Segalen - Bordeaux 2-Centre hospitalier Charles Perrens [Bordeaux]-Centre Expert Trouble Bipolaire, and Ikeda, Kazutaka
Subjects: Male, Bipolar Disorder, Lithium (medication), Psychometrics, Psychopharmacology, Epidemiology, International Cooperation, lcsh:Medicine, Lithium, Maintenance Treatment, ConLiGen, ddc:616.89, 0302 clinical medicine, Antimanic Agents, Psychology, lcsh:Science, Reliability (statistics), Psychiatry, Genetics, 0303 health sciences, Multidisciplinary, ASSOCIATION, Genomics, Pharmacoepidemiology, 3. Good health, Mental Health, Phenotype, Treatment Outcome, MAPPING SUSCEPTIBILITY GENES, Behavioral Pharmacology, RELIABILITY, Lithium Compounds, Medicine, Female, Research Article, medicine.drug, Drugs and Devices, MAPPING SUSCEPTIBILITY GENES, PROPHYLACTIC LITHIUM, OBSERVER AGREEMENT, MOOD DISORDERS, ONSET, ASSOCIATION, RELIABILITY, MORTALITY, ILLNESS, AGE, ILLNESS, PROPHYLACTIC LITHIUM, 03 medical and health sciences, AGE, Neuropharmacology, Genomic Medicine, [SDV.BBM] Life Sciences [q-bio]/Biochemistry, Molecular Biology, Genome-Wide Association Studies, medicine, Humans, [SDV.BBM]Life Sciences [q-bio]/Biochemistry, Molecular Biology, Bipolar disorder, ddc:610, Biology, 030304 developmental biology, Mood Disorders, business.industry, MORTALITY, lcsh:R, Computational Biology, Reproducibility of Results, Models, Theoretical, medicine.disease, OBSERVER AGREEMENT, Mood disorders, Pharmacogenetics, Therapies, ONSET, lcsh:Q, Pharmacogenomics, business, 030217 neurology & neurosurgery, Kappa
Abstract: Objective: The assessment of response to lithium maintenance treatment in bipolar disorder (BD) is complicated by variable length of treatment, unpredictable clinical course, and often inconsistent compliance. Prospective and retrospective methods of assessment of lithium response have been proposed in the literature. In this study we report the key phenotypic measures of the "Retrospective Criteria of Long-Term Treatment Response in Research Subjects with Bipolar Disorder" scale currently used in the Consortium on Lithium Genetics (ConLiGen) study. Materials and Methods: Twenty-nine ConLiGen sites took part in a two-stage case-vignette rating procedure to examine inter-rater agreement [Kappa (\(\kappa\))] and reliability [intra-class correlation coefficient (ICC)] of lithium response. Annotated first-round vignettes and rating guidelines were circulated to expert research clinicians for training purposes between the two stages. Further, we analyzed the distributional properties of the treatment response scores available for 1,308 patients using mixture modeling. Results: Substantial and moderate agreement was shown across sites in the first and second sets of vignettes (\(\kappa\) = 0.66 and \(\kappa\) = 0.54, respectively), without significant improvement from training. However, definition of response using the A score as a quantitative trait and selecting cases with B criteria of 4 or less showed an improvement between the two stages (\(ICC_1 = 0.71\) and \(ICC_2 = 0.75\), respectively). Mixture modeling of score distribution indicated three subpopulations (full responders, partial responders, non responders). Conclusions: We identified two definitions of lithium response, one dichotomous and the other continuous, with moderate to substantial inter-rater agreement and reliability. Accurate phenotypic measurement of lithium response is crucial for the ongoing ConLiGen pharmacogenomic study.
Published: 2013
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25. Endosymbiosis in trypanosomatids: the genomic cooperation between bacterium and host in the synthesis of essential amino acids is heavily influenced by multiple horizontal gene transfers

Author: Maria Cristina M. Motta, Marie-France Sagot, Myrna G. Serrano, João M. P. Alves, Cecilia C. Klein, Gregory A. Buck, Marta Maria Geraldes Teixeira, Flávia Maia da Silva, Ana Tereza Ribeiro de Vasconcelos, Erney P. Camargo, André G. Costa-Martins, Virginia Commonwealth University (VCU), Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Laboratorio Nacional de Computação Cientifica [Rio de Janeiro] (LNCC / MCT), An algorithmic view on genomes, cells, and environments (BAMBOO), Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Baobab, Département PEGASE [LBBE] (PEGASE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Laboratório de Ultraestrutura Celular Hertha Meyer - IBCCF / UFRJ [Rio de Janeiro], Instituto de Biofísica Carlos Chagas Filho [Rio de Janeiro] (IBCCF / UFRJ), Universidade Federal do Rio de Janeiro (UFRJ)-Universidade Federal do Rio de Janeiro (UFRJ), ANR-08-BLAN-0293,MIRI,Combinatorial exploration of the molecular landscape and evolution of intimate species relations(2008), European Project: 247073,EC:FP7:ERC,ERC-2009-AdG,SISYPHE(2010), and Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP)
Subjects: Ornithine cyclodeaminase, [SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Angomonas deanei, Gene Transfer, Horizontal, Biology, Genome, Amino acid biosynthesis, 03 medical and health sciences, Phylogenetics, Proteobacteria, Botany, parasitic diseases, Trypanosomatids, Symbiosis, Gene, Phylogeny, Ecology, Evolution, Behavior and Systematics, Amino acid synthesis, 030304 developmental biology, Genetics, chemistry.chemical_classification, 0303 health sciences, Endosymbiosis, 030306 microbiology, fungi, Betaproteobacteria, TRYPANOSOMA, biochemical phenomena, metabolism, and nutrition, Genomic analyses, biology.organism_classification, Biological Evolution, chemistry, Metabolic pathway evolution, Horizontal gene transfer, Trypanosomatina, Amino Acids, Essential, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Genome, Bacterial, Research Article
Abstract: International audience; BackgroundTrypanosomatids of the genera Angomonas and Strigomonas live in a mutualistic association characterized by extensive metabolic cooperation with obligate endosymbiotic Betaproteobacteria. However, the role played by the symbiont has been more guessed by indirect means than evidenced. Symbiont-harboring trypanosomatids, in contrast to their counterparts lacking symbionts, exhibit lower nutritional requirements and are autotrophic for essential amino acids. To evidence the symbiont's contributions to this autotrophy, entire genomes of symbionts and trypanosomatids with and without symbionts were sequenced here.ResultsAnalyses of the essential amino acid pathways revealed that most biosynthetic routes are in the symbiont genome. By contrast, the host trypanosomatid genome contains fewer genes, about half of which originated from different bacterial groups, perhaps only one of which (ornithine cyclodeaminase, EC:4.3.1.12) derived from the symbiont. Nutritional, enzymatic, and genomic data were jointly analyzed to construct an integrated view of essential amino acid metabolism in symbiont-harboring trypanosomatids. This comprehensive analysis showed perfect concordance among all these data, and revealed that the symbiont contains genes for enzymes that complete essential biosynthetic routes for the host amino acid production, thus explaining the low requirement for these elements in symbiont-harboring trypanosomatids. Phylogenetic analyses show that the cooperation between symbionts and their hosts is complemented by multiple horizontal gene transfers, from bacterial lineages to trypanosomatids, that occurred several times in the course of their evolution. Transfers occur preferentially in parts of the pathways that are missing from other eukaryotes.ConclusionWe have herein uncovered the genetic and evolutionary bases of essential amino acid biosynthesis in several trypanosomatids with and without endosymbionts, explaining and complementing decades of experimental results. We uncovered the remarkable plasticity in essential amino acid biosynthesis pathway evolution in these protozoans, demonstrating heavy influence of horizontal gene transfer events, from Bacteria to trypanosomatid nuclei, in the evolution of these pathways.
Published: 2013
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26. Biosynthesis of vitamins and cofactors in bacterium-harbouring trypanosomatids depends on the symbiotic association as revealed by genomic analyses

Author: Cecilia C. Klein, Myrna G. Serrano, João M. P. Alves, Maria Cristina M. Motta, Marta Maria Geraldes Teixeira, Erney P. Camargo, Marie-France Sagot, Ana Tereza Ribeiro de Vasconcelos, Gregory A. Buck, Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), An algorithmic view on genomes, cells, and environments (BAMBOO), Inria Grenoble - Rhône-Alpes, Institut National de Recherche en Informatique et en Automatique (Inria)-Institut National de Recherche en Informatique et en Automatique (Inria)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL), Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS), Department of Parasitology [São Paulo] (IBS), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), Virginia Commonwealth University (VCU), Laboratorio Nacional de Computação Cientifica [Rio de Janeiro] (LNCC / MCT), Baobab, Département PEGASE [LBBE] (PEGASE), Université de Lyon-Université de Lyon-Institut National de Recherche en Informatique et en Automatique (Inria)-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE), Instituto de Biofísica Carlos Chagas Filho [Rio de Janeiro] (IBCCF / UFRJ), Universidade Federal do Rio de Janeiro (UFRJ), ANR-08-BLAN-0293,MIRI,Combinatorial exploration of the molecular landscape and evolution of intimate species relations(2008), European Project: 247073,EC:FP7:ERC,ERC-2009-AdG,SISYPHE(2010), and Universidade de São Paulo (USP)-Universidade de São Paulo (USP)
Subjects: Angomonas deanei, Trypanosoma, PARASITOLOGIA, Science, 030231 tropical medicine, [SDV.BID]Life Sciences [q-bio]/Biodiversity, Genome, 03 medical and health sciences, Biological Factors, 0302 clinical medicine, Alcaligenaceae, parasitic diseases, Symbiosis, Gene, Phylogeny, 030304 developmental biology, 2. Zero hunger, Genetics, 0303 health sciences, Multidisciplinary, biology, fungi, Betaproteobacteria, Genomics, Vitamins, Vitamin biosynthesis, biology.organism_classification, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Biosynthetic Pathways, Biochemistry, Horizontal gene transfer, Medicine, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], Genome, Protozoan, Bacteria, Research Article
Abstract: International audience; Some non-pathogenic trypanosomatids maintain a mutualistic relationship with a betaproteobacterium of the Alcaligenaceae family. Intensive nutritional exchanges have been reported between the two partners, indicating that these protozoa are excellent biological models to study metabolic co-evolution. We previously sequenced and herein investigate the entire genomes of five trypanosomatids which harbor a symbiotic bacterium (SHTs for Symbiont-Haboring Trypanosomatids) and the respective bacteria (TPEs for Trypanosomatid Proteobacterial Endosymbiont), as well as two trypanosomatids without symbionts (RTs for Regular Trypanosomatids), for the presence of genes of the classical pathways for vitamin biosynthesis. Our data show that genes for the biosynthetic pathways of thiamine, biotin, and nicotinic acid are absent from all trypanosomatid genomes. This is in agreement with the absolute growth requirement for these vitamins in all protozoa of the family. Also absent from the genomes of RTs are the genes for the synthesis of pantothenic acid, folic acid, riboflavin, and vitamin B6. This is also in agreement with the available data showing that RTs are auxotrophic for these essential vitamins. On the other hand, SHTs are autotrophic for such vitamins. Indeed, all the genes of the corresponding biosynthetic pathways were identified, most of them in the symbiont genomes, while a few genes, mostly of eukaryotic origin, were found in the host genomes. The only exceptions to the latter are: the gene coding for the enzyme ketopantoate reductase (EC:1.1.1.169) which is related instead to the Firmicutes bacteria; and two other genes, one involved in the salvage pathway of pantothenic acid and the other in the synthesis of ubiquinone, that are related to Gammaproteobacteria. Their presence in trypanosomatids may result from lateral gene transfer. Taken together, our results reinforce the idea that the low nutritional requirement of SHTs is associated with the presence of the symbiotic bacterium, which contains most genes for vitamin production.
Published: 2013
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27. Synthesis and dopamine receptor binding of dihydrexidine and SKF 38393 catecholamine-based analogues

Author: Christian Pifl, Marcus Wieder, Suzane Rosa da Silva, Martin Zehl, Gert Lubec, Anna Fabišiková, Luana Ribeiro Dos Anjos, Alexander Prado-Roller, Natalie Gajic, Vladimir Dragačević, Philip John Neill, Vinicius Palaretti, Predrag Kalaba, Eduardo René Pérez González, Gil Valdo José da Silva, Universidade Estadual Paulista (UNESP), University of Vienna, Universidade de São Paulo (USP), Medical University of Vienna, and Paracelsus Medical University
Subjects: Clinical Biochemistry, D1R agonists, Biochemistry, Dihydrexidine, Reuptake, chemistry.chemical_compound, Catecholamines, Dopamine, Dopamine receptor D2, medicine, Neurotransmitter, Receptor, Dopamine analogs, Dopamine transporter, biology, Chemistry, Receptors, Dopamine D1, Organic Chemistry, D2R and DAT, Dopamine receptor binding, Phenanthridines, biology.protein, 2,3,4,5-Tetrahydro-7,8-dihydroxy-1-phenyl-1H-3-benzazepine, SKF 38393, medicine.drug
Abstract: Made available in DSpace on 2022-04-28T19:47:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Dopamine is an important neurotransmitter that regulates numerous essential functions, including cognition and voluntary movement. As such, it serves as an important scaffold for synthesis of novel analogues as part of drug development effort to obtain drugs for treatment of neurodegenerative diseases, such as Parkinson's disease. To that end, similarity search of the ZINC database based on two known dopamine-1 receptor (D1R) agonists, dihydrexidine (DHX) and SKF 38393, respectively, was used to predict novel chemical entities with potential binding to D1R. Three compounds that showed the highest similarity index were selected for synthesis and bioactivity profiling. All main synthesis products as well as the isolated intermediates, were properly characterized. The physico-chemical analyses were performed using HRESIMS, GC/MS, LC/MS with UV–Vis detection, and FTIR, 1H NMR and 13C NMR spectroscopy. Binding to D1 and D2 receptors and inhibition of dopamine reuptake via dopamine transporter were measured for the synthesized analogues of DHX and SKF 38393. Laboratory of Fine Organic Chemistry Department of Chemistry and Biochemistry Faculty of Sciences and Technology of São Paulo State University, São Paulo Department of Pharmaceutical Chemistry Faculty of Life Sciences University of Vienna, Althanstraße 14 Mass Spectrometry Centre Faculty of Chemistry University of Vienna, Währinger Straße 38 Department of Analytical Chemistry Faculty of Chemistry University of Vienna, Währinger Straße 38 Centre for X-Ray Structure Analysis at Faculty of Chemistry University of Vienna, Währinger Straße 40-42 Department of Chemistry Faculty of Philosophy Sciences and Letters of Ribeirão Preto University of São Paulo, Avenida dos Bandeirantes, 3900, SP Department of Molecular Neurosciences Centre for Brain Research Medical University of Vienna, Spitalgasse 4 Department of Neuroproteomics Paracelsus Medical University Laboratory of Fine Organic Chemistry Department of Chemistry and Biochemistry Faculty of Sciences and Technology of São Paulo State University, São Paulo FAPESP: 2016/10149-0
Published: 2021
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28. High chromosomal mobility of r <scp>DNA</scp> clusters in holocentric chromosomes of Triatominae, vectors of Chagas disease ( <scp>Hemiptera‐Reduviidae</scp> )

Author: Kaio Cesar Chaboli Alevi, Margarita Cabrera-Bravo, Yanina Panzera, Angeles Cuadrado, Pedro Lorite, João Aristeu da Rosa, Carlota Monroy, Aldo Solari, Francisco Panzera, Simone Patrícia Carneiro Freitas, Andrés Gómez-Palacio, J. A. de Oliveira, Patricia L. Dorn, Sebastián Pita, Universidad de la República, University of Jaén, University of Alcalá, Universidade de São Paulo (USP), Universidade Estadual Paulista (UNESP), Fundação Oswaldo Cruz, Universidad Pedagógica y Tecnológica de Colombia, Universidad de Chile, Universidad de San Carlos de Guatemala, Loyola University New Orleans, and Universidad Nacional Autónoma de México
Subjects: Subfamily, Autosome, General Veterinary, Chromosome, Context (language use), Biology, biology.organism_classification, DNA, Ribosomal, Chromosomes, Reduviidae, Evolutionary biology, Insect Science, Holocentric, Animals, Chagas Disease, Parasitology, Triatominae, Ecology, Evolution, Behavior and Systematics, X chromosome
Abstract: Made available in DSpace on 2022-04-28T19:46:42Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Agencia Nacional de Investigación e Innovación American University University of Arizona University of Vermont Junta de Andalucía Comisión Sectorial de Investigación Científica Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Ministerio de Educación y Cultura The subfamily Triatominae (Hemiptera-Reduviidae) includes more than 150 blood-sucking species, potential vectors of the protozoan Trypanosoma cruzi, causative agent of Chagas disease. A distinctive cytogenetic characteristic of this group is the presence of extremely stable chromosome numbers. Unexpectedly, the analyses of the chromosomal location of ribosomal gene clusters and other repetitive sequences place Triatominae as a significantly diverse hemipteran subfamily. Here, we advance the understanding of Triatominae chromosomal evolution through the analysis of the 45S rDNA cluster chromosomal location in 92 Triatominae species. We found the 45S rDNA clusters in one to four loci per haploid genome with different chromosomal patterns: On one or two autosomes, on one, two or three sex chromosomes, on the X chromosome plus one to three autosomes. The movement of 45S rDNA clusters is discussed in an evolutionary context. Our results illustrate that rDNA mobility has been relatively common in the past and in recent evolutionary history of the group. The high frequency of rDNA patterns involving autosomes and sex chromosomes among closely related species could affect genetic recombination and the viability of hybrid populations, which suggests that the mobility of rDNA clusters could be a driver of species diversification. Sección Genética Evolutiva Facultad de Ciencias Universidad de la República Department of Experimental Biology Genetics University of Jaén Department of Biomedicine and Biotechnology University of Alcalá Laboratório de Entomologia em Saúde Pública Departamento de Epidemiologia Faculdade de Saúde Pública Universidade de São Paulo Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista “Júlio de Mesquita Filho” (Unesp) Fundação Oswaldo Cruz Laboratorio de Investigación en Genética Evolutiva – LIGE Universidad Pedagógica y Tecnológica de Colombia Programa de Biología Celular y Molecular ICBM Facultad de Medicina Universidad de Chile Laboratorio de Entomología Aplicada y Parasitología Escuela de Biología Facultad de Farmacia Universidad de San Carlos de Guatemala Department of Biological Sciences Loyola University New Orleans Departamento de Microbiología y Parasitología Facultad de Medicina Universidad Nacional Autónoma de México Faculdade de Ciências Farmacêuticas Universidade Estadual Paulista “Júlio de Mesquita Filho” (Unesp) Comisión Sectorial de Investigación Científica: 160 FAPESP: 17/05015-7 FAPESP: 19/02145-2 FAPESP: 307 398/2018-8 Ministerio de Educación y Cultura: II/FVF/2019/054
Published: 2021
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29. Lipid microspheres containing urea for slow release of non-protein N in ruminant diets

Author: Rob Dixon, Antonio Joelson Netto, Diego Lomonaco Vasconcelos De Oliveira, Leilson Rocha Bezerra, Ariádne de Barros Carvalho, Jéssica Ribeiro Oliveira, Selma Elaine Mazzetto, Ronaldo Lopes Oliveira, Aderbal Marcos de Azevedo Silva, Deuber Lincon da Silva Agostini, André Leandro da Silva, José Morais Pereira Filho, Lloyd Ryan Viana Kotzebue, Federal University of Campina Grande, Universidade de São Paulo (USP), Federal University of Ceará, and Universidade Federal da Bahia (UFBA)
Subjects: agricultural innovations, sheep nutrition, biology, food chemistry, chemistry.chemical_element, Context (language use), biology.organism_classification, Nitrogen, Feed conversion ratio, nitrogen, chemistry.chemical_compound, Rumen, carnauba wax, Animal science, chemistry, ruminants, Ruminant, Toxicity, Urea, microencapsulation, Animal Science and Zoology, slow-release urea, Carnauba wax, Food Science
Abstract: Made available in DSpace on 2022-04-29T08:46:01Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Context: Urea is widely used in ruminant diets as a source of non-protein nitrogen (NPN), partially substituting for true protein in feed, but high levels of urea in the diet may cause toxicity. Aims: This study investigated the microencapsulation of urea in carnauba wax for slow release in the rumen to improve the N efficiency and growth of sheep. Methods: Two microencapsulated systems were developed with urea:carnauba wax ratios (w/w) of 1:2 (U12) and 1:4 (U14). Based on the initial characterisation, only U12 was examined in an in vivo experiment with Santa Ines crossbred male sheep (n = 40) initially weighing 28 ± 0.6 kg at 270 days of age. The experimental arrangement was a completely randomised design, and the animals were distributed into four treatments i.e. four levels of inclusion (0, control 15 30 and 45 g/kg DM) of microencapsulated urea in the diet. Key results: The dietary inclusion of microencapsulated urea was associated with linear increases (P ≤ 0.05) in the intake of DM and metabolisable energy, the digestibility of crude protein, non-fibrous carbohydrates and fibre fractions, and N balance. Additionally, liveweight gain and feed efficiency increased quadratically (P < 0.001). There were also linear reductions (P < 0.001) in blood urea and urinary urea concentrations. Conclusions: The study demonstrated that the inclusion of up to 30 g/kg U12 microencapsulated urea can be recommended as a source of slow-release urea in sheep diets since it improved the performance and feed efficiency and promoted lower concentrations of blood urea and urinary urea. Implications: The use of urea microencapsulated in carnauba wax can reduce the risk of urea toxicity and provide a safer way to supply NPN to ruminants and improve N utilisation. Department of Animal Science Federal University of Campina Grande, Patos, Avenue Universitária, Caixa Postal 61 Department of Physics Chemistry and Biology State University of São Paulo, Presidente Prudente, Rua Roberto Simonsen, 305 Department of Organic and Inorganic Chemistry Federal University of Ceará, Rua do Contorno, , Ceará Department of Animal Science Federal University of Bahia, Ondina, Bahia, Avenue Adhemar de Barros, 500
Published: 2021
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30. Engineered asparaginase from <scp> Erwinia chrysanthemi </scp> enhances asparagine hydrolase activity and diminishes enzyme immunoreactivity ‐ a new promise to treat acute lymphoblastic leukemia

Author: Guilherme Meira Lima, Gisele Monteiro, Débora Custodio Moura, Camila O. dos Santos, Marcos Antonio de Oliveira, Iris Munhoz Costa, Adalberto Pessoa, Universidade de São Paulo (USP), Cold Spring Harbor, and Universidade Estadual Paulista (UNESP)
Subjects: immunogenic epitopes, lower therapeutic ASNase dose, Asparaginase, l-asparaginase, General Chemical Engineering, Pharmacology, Epitope, Inorganic Chemistry, chemistry.chemical_compound, medicine, Erwinia chrysanthemi, Asparagine, Waste Management and Disposal, chemistry.chemical_classification, biology, Renewable Energy, Sustainability and the Environment, Chemistry, Organic Chemistry, protein engineering, medicine.disease, Pollution, Enzyme assay, Leukemia, Fuel Technology, Enzyme, Toxicity, biology.protein, Specific activity, mutation, Biotechnology
Abstract: Made available in DSpace on 2022-04-28T19:45:29Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-01-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) BACKGROUND: The treatment of acute lymphoblastic leukemia (ALL) uses the biopharmaceutical l-asparaginase (ASNase) as the main medication. This drug, from bacterial origin (Escherichia coli or Erwinia chrysanthemi), depletes l-asparagine (Asn) and secondarily l-glutamine (Gln – GLNase activity) from the bloodstream, leading leukemic cells to die by deprivation of Asn. The use of ASNase is limited by the high incidence of adverse effects, which collectively can specifically impair quality of life of patients. Its high toxicity caused by the product of the hydrolysis of amino acids and the formation of anti-ASNase antibodies often required treatment interruption, thus reducing the chances of cure and increasing the rates of disease relapse. RESULTS: In order to improve enzymatic activity, while reducing toxicity, we developed through directed evolution a double-mutant ASNase from Erwinia chrysanthemi (Erw_DM), which has specific activity for Asn 46% higher than the wild-type enzyme (Erw_WT). This makes it possible to reduce the amount of protein necessary for depletion of this amino acid and, consequently, the reduction of GLNase activity, considered toxic. In silico analysis showed that a lower number of epitopes was exposed, resulting in reduced recognition of the recombinant protein by antibody anti-ASNase observed in vitro assay. Furthermore, we observed the same cytotoxic profile for the MOLT-4 and REH ALL cell lines using 40% lower protein mass of Erw_DM to achieve the minimum enzyme activity required in the bloodstream during treatment. CONCLUSION: Altogether, our findings describe a potent and less immunogenic ASNase, an improvement that may alleviate treatment adverse effects developed in anti-ALL therapy. © 2021 Society of Chemical Industry (SCI). Departamento de Tecnologia Bioquímico-Farmacêutica Faculdade de Ciências Farmacêuticas Universidade de São Paulo Cold Spring Harbor Laboratory Cold Spring Harbor Instituto de Biociências Universidade Estadual Paulista Instituto de Biociências Universidade Estadual Paulista CAPES: 001 FAPESP: 2013/08617-7 FAPESP: 2015/07749-2 FAPESP: 2016/25896-5 FAPESP: 2018/15104-0 CNPq: 309224/2019-5
Published: 2021
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31. Mites (Mesostigmata: Melicharidae) associated with hummingbirds (Aves: Trochilidae) in Brazil

Author: Fernando de Castro Jacinavicius, Ashley P. G. Dowling, Erich Fischer, Gilberto J. de Moraes, Claudenice Faxina, Darci Moraes Barros-Battesti, Luciano Moreira-Lima, J. C. Takatsu, Laura Contreras Peinado, J. C. Santos, Erika Hingst-Zaher, R. Bassini-Silva, Instituto Butantan, Universidade Estadual Paulista (UNESP), Universidade Federal de Mato Grosso do Sul (UFMS), Independent Researcher, Universidade de São Paulo (USP), and University of Arkansas
Subjects: Melicharid mites, phoresy, Trochilidae, Insect Science, BEIJA-FLORES, Zoology, Mesostigmata, Neotropical region, Melicharidae, Biology, biology.organism_classification, Brazil
Abstract: Made available in DSpace on 2022-04-29T08:34:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Mites of the family Melicharidae comprise 13 genera and 220 species often associated with edaphic environments and found in association with insects, birds and mammals. Two genera, Proctolaelaps and Tropicoseius, have been recorded in Brazil in association with birds and bromeliads. In this study, we add new locality and host-association records for Proctolaelaps superagui and Tropicoseius braziliensis in the country. Laboratório de Coleções Zoológicas Instituto Butantan Departamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias-UNESP Laboratório de Ecologia Instituto de Biologia Universidade Federal de Mato Grosso do Sul Museu Biológico Instituto Butantan Independent Researcher Departamento de Entomologia e Acarologia ESALQ-Universidade de São Paulo Department of Entomology University of Arkansas Departamento de Patologia Reprodução e Saúde Única Faculdade de Ciências Agrárias e Veterinárias-UNESP
Published: 2021
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32. Molecular basis of a bacterial-amphibian symbiosis revealed by comparative genomics, modeling, and functional testing

Author: Célio F. B. Haddad, Mariana L. Lyra, Cathrin Spröer, Andrés Eduardo Brunetti, Boyke Bunk, Carlos Alessandro Fuzo, Mariela M. Marani, Jörg Overmann, Norberto Peporine Lopes, Universidade de São Paulo (USP), Universidad Nacional de Misiones, Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures, Universidade Estadual Paulista (UNESP), Consejo Nacional de Investigaciones Científicas y Técnicas, and Technische Universität Braunschweig
Subjects: Genetics, Comparative genomics, Whole genome sequencing, Bacteria, biology, Pseudomonas, Antimicrobial peptides, Genomics, Bacterial genome size, biology.organism_classification, Microbiology, Genome, Article, Animals, Microbiome, Anura, Symbiosis, Gene, Genome, Bacterial, Ecology, Evolution, Behavior and Systematics
Abstract: Made available in DSpace on 2022-04-29T08:34:55Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-03-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) The molecular bases for the symbiosis of the amphibian skin microbiome with its host are poorly understood. Here, we used the odor-producer Pseudomonas sp. MPFS and the treefrog Boana prasina as a model to explore bacterial genome determinants and the resulting mechanisms facilitating symbiosis. Pseudomonas sp. MPFS and its closest relatives, within a new clade of the P. fluoresens Group, have large genomes and were isolated from fishes and plants, suggesting environmental plasticity. We annotated 16 biosynthetic gene clusters from the complete genome sequence of this strain, including those encoding the synthesis of compounds with known antifungal activity and of odorous methoxypyrazines that likely mediate sexual interactions in Boana prasina. Comparative genomics of Pseudomonas also revealed that Pseudomonas sp. MPFS and its closest relatives have acquired specific resistance mechanisms against host antimicrobial peptides (AMPs), specifically two extra copies of a multidrug efflux pump and the same two-component regulatory systems known to trigger adaptive resistance to AMPs in P. aeruginosa. Subsequent molecular modeling indicated that these regulatory systems interact with an AMP identified in Boana prasina through the highly acidic surfaces of the proteins comprising their sensory domains. In agreement with a symbiotic relationship and a highly selective antibacterial function, this AMP did not inhibit the growth of Pseudomonas sp. MPFS but inhibited the growth of another Pseudomonas species and Escherichia coli in laboratory tests. This study provides deeper insights into the molecular interaction of the bacteria-amphibian symbiosis and highlights the role of specific adaptive resistance toward AMPs of the hosts. Departamento de Ciências Biomoleculares Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, SP Laboratorio de Genética Evolutiva Instituto de Biología Subtropical (CONICET – UNaM) Facultad de Ciencias Exactas Universidad Nacional de Misiones Leibniz Institute DSMZ-German Collection of Microorganisms and Cell Cultures Departamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista, SP Departamento de Análises Clínicas Toxicológicas e Bromatológicas Faculdade de Ciências Farmacêuticas de Ribeirão Preto Universidade de São Paulo, SP IPEEC-CONICET Instituto Patagónico para el Estudio de los Ecosistemas Continentales Consejo Nacional de Investigaciones Científicas y Técnicas Mikrobiologie Technische Universität Braunschweig Departamento de Biodiversidade e Centro de Aquicultura Instituto de Biociências Universidade Estadual Paulista, SP FAPESP: 2013/50741-7 FAPESP: 2013/50954-0 FAPESP: 2014/20915-6 FAPESP: 2014/50265-3 FAPESP: 2017/23725-1 FAPESP: 2017/26162-8 CAPES: 88881.062205/2014-01 CAPES: 88887.464731/2019-00
Published: 2021
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33. Ultrastructure of the adrenal gland of paca (Cuniculus paca, Linnaeus, 1766) in captivity

Author: Paulo Fernandes Marcusso, Leandro Luis Martins, Tais Harumi de Castro Sasahara, Sergio Pinter Garcia Filho, Márcia Rita Fernandes Machado, Universidade Estadual de Maringá (UEM), Universidade Estadual de Londrina (UEL), Universidade Federal dos Vales do Jequitinhonha e Mucuri, Universidade de São Paulo (USP), and Universidade Estadual Paulista (UNESP)
Subjects: Rodent, General Veterinary, endocrine system diseases, electron microscopy, Adrenal gland, Veterinary medicine, rodent, Captivity, Suprarenal, Anatomy, Biology, Comparative anatomy, biology.organism_classification, Wild animals, medicine.anatomical_structure, suprarenal, biology.animal, wild animals, SF600-1100, Electron microscopy, Ultrastructure, medicine, Paca
Abstract: Made available in DSpace on 2022-04-29T08:35:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-30 Lowland paca (Cuniculus paca, Linnaeus, 1766) is a medium-sized rodent that belongs to the Brazilian fauna. Yet little information on its morphology is found in the specialized literature. Thus, the objective of the work was to study the morphology of the adrenal gland of paca by means of microscopic ultrastructure analysis. The adrenal gland secretes specialized substances in the body which promote biological functions of great importance and will provide valuable information to studies in comparative anatomy. Two (2) adult lowland pacas were used, male and female. Soon after death, the animals were positioned in the supine position; their abdominal cavities were opened by pre-retro umbilical and lateral incision followed by folding of the abdominal walls to expose the glands. The adrenal glands were removed; fragments were collected, fixed and prepared for ultrastructure observations using scanning electron microscopy and transmission electron microscopy techniques. It was observed that the adrenal glands of the paca have divisions as well as the limits of the cortical and medullary region, as well as the subdivisions of the glomerulosa, fasciculated and reticulated areas of the cortical region as in other rodents. An ultrastructure of cells and their components also showed a lot of similarity to that already demonstrated in different rodents. Departamento de Medicina Veterinária Universidade Estadual de Maringá, PR Departamento de Anatomia Universidade Estadual de Londrina, PR Instituto de Ciências Agrárias e Veterinária Universidade Federal dos Vales do Jequitinhonha e Mucuri, MG Faculdade de Medicina Veterinária e Zootecnia Universidade de São Paulo, SP Departamento de Morfologia Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, SP Departamento de Morfologia Animal Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista, SP
Published: 2021

34. Transcriptome dynamics during metamorphosis of imaginal discs into wings and thoracic dorsum in Apis mellifera castes

Author: Zilá Luz Paulino Simões, Flávia Cristina de Paula Freitas, Márcia Maria Gentile Bitondi, Michelle P.M. Soares, Daniel Guariz Pinheiro, Universidade de São Paulo (USP), Universidade Estadual Paulista (UNESP), and FIOCRUZ
Subjects: Caste differential expression, animal structures, media_common.quotation_subject, Honeybee, RNA-Seq, Insect, Biology, QH426-470, Gene expression, Genetics, Animals, Humans, Wings, Animal, Metamorphosis, media_common, miRNA, Larva, Wing, Research, Metamorphosis, Biological, Bees, biology.organism_classification, Wing imaginal discs, Imaginal disc, Imaginal Discs, Social Class, Evolutionary biology, Queen (butterfly), Apis mellifera, RNA-seq, Transcriptome, TP248.13-248.65, Biotechnology
Abstract: Made available in DSpace on 2022-04-29T08:35:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Background: Much of the complex anatomy of a holometabolous insect is built from disc-shaped epithelial structures found inside the larva, i.e., the imaginal discs, which undergo a rapid differentiation during metamorphosis. Imaginal discs-derived structures, like wings, are built through the action of genes under precise regulation. Results: We analyzed 30 honeybee transcriptomes in the search for the gene expression needed for wings and thoracic dorsum construction from the larval wing discs primordia. Analyses were carried out before, during, and after the metamorphic molt and using worker and queen castes. Our RNA-seq libraries revealed 13,202 genes, representing 86.2% of the honeybee annotated genes. Gene Ontology analysis revealed functional terms that were caste-specific or shared by workers and queens. Genes expressed in wing discs and descendant structures showed differential expression profiles dynamics in premetamorphic, metamorphic and postmetamorphic developmental phases, and also between castes. At the metamorphic molt, when ecdysteroids peak, the wing buds of workers showed maximal gene upregulation comparatively to queens, thus underscoring differences in gene expression between castes at the height of the larval-pupal transition. Analysis of small RNA libraries of wing buds allowed us to build miRNA-mRNA interaction networks to predict the regulation of genes expressed during wing discs development. Conclusion: Together, these data reveal gene expression dynamics leading to wings and thoracic dorsum formation from the wing discs, besides highlighting caste-specific differences during wing discs metamorphosis. Departamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, Av. Bandeirantes 3900 Departamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho Instituto Carlos Chagas FIOCRUZ Departamento de Biologia Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, Av. Bandeirantes 3900 Departamento de Tecnologia Faculdade de Ciências Agrárias e Veterinárias Universidade Estadual Paulista Júlio de Mesquita Filho
Published: 2021

35. IFN-γ Mediated Signaling Improves Fungal Clearance in Experimental Pulmonary Mucormycosis

Author: Vanessa Soares Lara, James Venturini, Thais Fernanda de Campos Fraga-Silva, Cleverson Teixeira Soares, Maria Izilda Andrade, Olavo Speranza de Arruda, Nara Ligia Martins Almeida, Rodolfo Ferreira dos Santos, Débora de Fátima Almeida-Donanzam, Maria Sueli Parreira de Arruda, Angela Carolina Finato, Amanda Ribeiro dos Santos, Universidade Estadual Paulista (UNESP), Universidade Federal de Mato Grosso do Sul (UFMS), Universidade de São Paulo (USP), and Lauro de Souza Lima Institute
Subjects: Fungal infection, medicine.medical_specialty, Veterinary (miscellaneous), medicine.medical_treatment, Rhizopus oryzae, Spleen, Applied Microbiology and Biotechnology, Microbiology, IFN-γ −/− mice, Mice, Medical microbiology, C57bl/6 mice, Swiss mice, medicine, Splenocyte, BALB/c mice, Animals, Mucormycosis, Lung, Kidney, INFECÇÕES RESPIRATÓRIAS, Mice, Inbred BALB C, biology, Inoculation, Immunosuppression, biology.organism_classification, Acquired immune system, Mice, Inbred C57BL, medicine.anatomical_structure, Original Article, Agronomy and Crop Science, Rhizopus
Abstract: Made available in DSpace on 2022-04-29T08:46:04Z (GMT). No. of bitstreams: 0 Previous issue date: 2022-02-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) We established three immunocompetent murine models of pulmonary mucormycosis to determine the involvement of the adaptive immune response in host resistance in pulmonary mucormycosis, a rapidly fatal disease caused mainly by Rhizopus spp. Immunocompetent inbred (C57BL/6, BALB/c) and outbred (Swiss) strains of mice were inoculated with R. oryzae via the intratracheal route. The inoculation resulted in a disseminated infection that spread to the brain, spleen, kidney, and liver. After 7 and 30 days of R. oryzae infection, BALB/c mice showed the lowest fungal load and highest production of IFN-γ and IL-2 by splenocytes. Swiss mice showed a higher fungal load 30 days p.i. and was associated with a weak development of the Th-1 profile. To confirm our findings, R. oryzae-infected IFN-γ−/− mice were evaluated after 60 days, where the mice still showed viable fungi in the lungs. This study showed, for the first time, that pulmonary mucormycosis in three widely used mouse strains resulted in an acute fungal dissemination without immunosuppression whose outcome varies according to the genetic background of the mice. We also identified the partial role of IFN-γ in the efficient elimination of R. oryzae during pulmonary infection. Faculdade de Ciências Universidade Estadual Paulista (Unesp), SP Faculdade de Medicina Universidade Federal de Mato Grosso do Sul (UFMS), Cidade Universitária, Unit 9, MS Departamento de Bioquimica e Imunologia Universidade de São Paulo, Escola de Medicina de Ribeirão Preto, SP Lauro de Souza Lima Institute, SP Faculdade de Odontologia de Bauru (FOB) Universidade de São Paulo (USP), SP Faculdade de Ciências Universidade Estadual Paulista (Unesp), SP FAPESP: # 2013/15513-3
Published: 2021

36. Bottom-up effects of breeding tomato genotypes on behavioural responses and performance of Tetranychus evansi population

Author: Rogério Falleiros Carvalho, Patrice Jacob Savi, Daniel Júnior de Andrade, Gilberto J. de Moraes, Universidade Estadual Paulista (UNESP), and Universidade de São Paulo (USP)
Subjects: education.field_of_study, biology, fungi, Population, food and beverages, Trichomes, biology.organism_classification, Trichome, Horticulture, Spider mite, Genotype, Wild tomato, Acari, Resistant genotype, PEST analysis, Solanum, Tomato red spider mite, education, Life table parameters, Agronomy and Crop Science, Sustainable production
Abstract: Made available in DSpace on 2022-05-01T09:30:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The tomato red spider mite, TRSM, Tetranychus evansi Baker & Pritchard (Acari: Tetranychidae), is an invasive tomato pest in several countries, with potential to reduce yield by up to 90% in Africa. Solanum habrochaites, access PI 134417 is a wild tomato genotype resistant to several arthropod pests, including TRSM. There is an interest in increasing the resistance of a tomato genotype (Solanum lycopersicum cv. TLCV15) widely cultivated by smallholder western African farmers to TRSM, through interspecific crossings with that wild genotype. For this purpose, after obtaining the F1 progeny and as well as F2 (SPJ-10–2017) and BC1 back-crossed (SPJ-05–2018) genotypes selected for high glandular trichome densities, we characterized their resistance level to TRSM. We quantified the types and densities of trichomes on the abaxial surface of their leaflets, and examined the subsequent bottom-up effects of these progeny plants attributes on behaviour and demographic parameters of the mite. Our results showed that the densities of glandular trichomes inherited from the resistant genotype (PI 134417) by the progenies were highly variable, with types I, IV and VI being the most prevalent. The progeny SPJ-10–2017 was classified as resistant, while the progenies F1 and SPJ-05–2018 were classified as partially resistant. These findings constitute one of the first steps towards advancing breeding programs in African countries to obtain tomato genotypes resistant to TRSM, targeting more sustainable production. College of Agricultural and Veterinary Sciences São Paulo State University (UNESP) Luiz de Queiroz College of Agriculture University of São Paulo (ESALQ/USP) College of Agricultural and Veterinary Sciences São Paulo State University (UNESP)
Published: 2021
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37. Effects of triploid induction on innate immunity and hematology in Astyanax altiparanae

Author: Ricardo Luiz Moro de Sousa, Antônio Augusto Mendes Maia, Mateus M. Carriero, Fabiana Pilarski, Caroline Munhoz Meira, George Shigueki Yasui, José Augusto Senhorini, Nycolas Levy-Pereira, Universidade Estadual Paulista (UNESP), Universidade de São Paulo (USP), and Chico Mendes Institute of Biodiversity Conservation
Subjects: Fish Proteins, Male, 0301 basic medicine, medicine.medical_specialty, Erythrocytes, Phagocytosis, Interleukin-1beta, Spleen, Saccharomyces cerevisiae, Aquatic Science, Biology, Yellow-tail tetra, Andrology, 03 medical and health sciences, Immune system, Aquaculture, Transforming Growth Factor beta, Internal medicine, Leukocytes, medicine, Animals, Environmental Chemistry, Head Kidney, Hematologic Tests, Innate immune system, Hematology, Characidae, business.industry, fungi, food and beverages, Triploid induction, 04 agricultural and veterinary sciences, General Medicine, Triploidy, Immunity, Innate, 030104 developmental biology, medicine.anatomical_structure, Erythrocytes morphometry, 040102 fisheries, Cytokines, 0401 agriculture, forestry, and fisheries, Female, Ploidy, business
Abstract: Made available in DSpace on 2022-04-29T08:30:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Triploid induction is a promising biotechnique that could be used to enhance aquaculture yields in the near future. However, studies conducted with several fish species have demonstrated that the presence of an extra set of chromosomes may result in deleterious health effects. Furthermore, studies of fish immune responses still need to be conducted before these specimens can be readily commercialized. In the study presented herein, we evaluated the effects of triploid induction on hematology, erythrocyte morphometry and morphology, phagocytosis, and the expression levels of IL-1β and TGF-β using specimens of the Neotropical species, Astyanax altiparanae. In general, the cell counts of erythrocytes, leukocytes, and neutrophils in triploid fish were lower than those in diploid fish. The erythrocytes of triploid fish were larger than those found in diploid fish, but also demonstrated considerably higher frequencies of cellular and nuclear abnormalities. Although not statistically significant, triploid induction resulted in a phagocytic capacity (PC) 20% lower than that found with diploid fish. No notable differences were observed in phagocytic index (PI). Gene expression levels for the cytokine IL-1 were lower in tissues from the head kidney, liver, and spleen of triploid fish with respect to diploid fish. Gene expression levels of TGF-β were lower only in the spleen of triploids compared to diploids. In conclusion, triploid induction resulted in A. altiparanae specimens with immune impairments and potentially lower resistances to disease and low-quality environments. Laboratory of Microbiology and Parasitology of Aquatic Organisms (LAPOA) Aquaculture Center (CAUNESP) São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n Laboratory of Zootechnical Hygiene Department of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA) University of São Paulo (USP), Pirassununga, SP, Brazil. Av. Duque de Caxias Norte, 225, Pirassununga Laboratory of Parasitology Department of Veterinary Medicine Faculty of Animal Science and Food Engineering (FZEA) University of São Paulo (USP), Av. Duque de Caxias Norte, 225, Pirassununga Laboratory of Fish Biotechnology National Center for Research and Conservation of Continental Fish Chico Mendes Institute of Biodiversity Conservation, Rodovia Pref. Euberto Nemésio Pereira de Godoy, Pirassununga Laboratory of Microbiology and Parasitology of Aquatic Organisms (LAPOA) Aquaculture Center (CAUNESP) São Paulo State University (UNESP), Via de Acesso Prof. Paulo Donato Castellane s/n CAPES: 2016/19149
Published: 2021
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38. Autofluorescence-spectral imaging as an innovative method for rapid, non-destructive and reliable assessing of soybean seed quality

Author: André Dantas de Medeiros, Nielsen Moreira Oliveira, Marcia Eugenia Amaral Carvalho, André Rodrigues dos Reis, Clíssia Barboza da Silva, Marina Lima Nogueira, Universidade de São Paulo (USP), Universidade Federal de Viçosa (UFV), and Universidade Estadual Paulista (UNESP)
Subjects: medicine.medical_specialty, media_common.quotation_subject, Science, Population, Biology, Biochemistry, Article, Machine Learning, medicine, Quality (business), education, media_common, education.field_of_study, Respiratory tract diseases, Multidisciplinary, Artificial neural network, Drug discovery, Optical Imaging, food and beverages, Agriculture, Linear discriminant analysis, Spectral imaging, Enzymes, Support vector machine, Autofluorescence, Germination, Seedlings, Seeds, Infectious diseases, Medicine, Soybeans, Biological system, Structural biology
Abstract: Made available in DSpace on 2022-04-28T19:44:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-12-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) In the agricultural industry, advances in optical imaging technologies based on rapid and non-destructive approaches have contributed to increase food production for the growing population. The present study employed autofluorescence-spectral imaging and machine learning algorithms to develop distinct models for classification of soybean seeds differing in physiological quality after artificial aging. Autofluorescence signals from the 365/400 nm excitation-emission combination (that exhibited a perfect correlation with the total phenols in the embryo) were efficiently able to segregate treatments. Furthermore, it was also possible to demonstrate a strong correlation between autofluorescence-spectral data and several quality indicators, such as early germination and seed tolerance to stressful conditions. The machine learning models developed based on artificial neural network, support vector machine or linear discriminant analysis showed high performance (0.99 accuracy) for classifying seeds with different quality levels. Taken together, our study shows that the physiological potential of soybean seeds is reduced accompanied by changes in the concentration and, probably in the structure of autofluorescent compounds. In addition, altering the autofluorescent properties in seeds impact the photosynthesis apparatus in seedlings. From the practical point of view, autofluorescence-based imaging can be used to check modifications in the optical properties of soybean seed tissues and to consistently discriminate high-and low-vigor seeds. Center for Nuclear Energy in Agriculture (CENA) University of São Paulo (USP) Department of Crop Science College of Agriculture Luiz de Queiroz (ESALQ) University of São Paulo (USP) Department of Genetics College of Agriculture Luiz de Queiroz (ESALQ) University of São Paulo (USP) Department of Agronomy Federal University of Viçosa (UFV) Department of Biosystems Engineering School of Sciences and Engineering São Paulo State University (UNESP) Department of Biosystems Engineering School of Sciences and Engineering São Paulo State University (UNESP) FAPESP: 2017/15220-7
Published: 2021

39. Proteomic analysis of chloroplast-to-chromoplast transition in tomato reveals metabolic shifts coupled with disrupted thylakoid biogenesis machinery and elevated energy-production components

Author: Cristina Barsan, Michel Rossignol, Eduardo Purgatto, Mohamed Zouine, Jean-Claude Pech, Isabel Egea, Alain Latché, Wanping Bian, Mondher Bouzayen, David Bouyssié, Carole Pichereaux, Elie Maza, Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées, Centre National de la Recherche Scientifique (CNRS), Institut de pharmacologie et de biologie structurale (IPBS), Centre National de la Recherche Scientifique (CNRS)-Université Toulouse III - Paul Sabatier (UT3), Universidade de São Paulo (USP), Laboratoire d'Excellence [ANR-10-LABX-41], Fundacion Seneca (Murcia, Spain), French Embassy in Bucharest (Romania), University of Chongqing (China), Government of Brazil (Conselho Nacional de Desenvolvimento Cientifico e Tecnolologico), Fondation pour la Recherche Médicale, Genopole Toulouse Midi-Pyrenees, Midi-Pyrenees Regional Council, Centre National de la Recherche Scientifique - CNRS (FRANCE), Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Platform Toulouse Genopole (FRANCE), Université Toulouse III - Paul Sabatier - UT3 (FRANCE), Universidade de São Paulo - USP (BRAZIL), and Institut National Polytechnique de Toulouse - INPT (FRANCE)
Subjects: 0106 biological sciences, Proteomics, Génomique, Transcriptomique et Protéomique, Physiology, [SDV]Life Sciences [q-bio], Plant Science, Biology, 01 natural sciences, Chloroplast, Tomato, 03 medical and health sciences, Chromoplast, Génétique des plantes, Genetics, Plastid, 030304 developmental biology, Photosystem, 2. Zero hunger, 0303 health sciences, food and beverages, Metabolism, Biochemistry, Thylakoid, Proteome, Biogenesis, 010606 plant biology & botany
Abstract: Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; International audience; A comparative proteomic approach was performed to identify differentially expressed proteins in plastids at three stages of tomato (Solanum lycopersicum) fruit ripening (mature-green, breaker, red). Stringent curation and processing of the data from three independent replicates identified 1,932 proteins among which 1,529 were quantified by spectral counting. The quantification procedures have been subsequently validated by immunoblot analysis of six proteins representative of distinct metabolic or regulatory pathways. Among the main features of the chloroplast-to-chromoplast transition revealed by the study, chromoplastogenesis appears to be associated with major metabolic shifts: (1) strong decrease in abundance of proteins of light reactions (photosynthesis, Calvin cycle, photorespiration) and carbohydrate metabolism (starch synthesis/degradation), mostly between breaker and red stages and (2) increase in terpenoid biosynthesis (including carotenoids) and stress-response proteins (ascorbate-glutathione cycle, abiotic stress, redox, heat shock). These metabolic shifts are preceded by the accumulation of plastid-encoded acetyl Coenzyme A carboxylase D proteins accounting for the generation of a storage matrix that will accumulate carotenoids. Of particular note is the high abundance of proteins involved in providing energy and in metabolites import. Structural differentiation of the chromoplast is characterized by a sharp and continuous decrease of thylakoid proteins whereas envelope and stroma proteins remain remarkably stable. This is coincident with the disruption of the machinery for thylakoids and photosystem biogenesis (vesicular trafficking, provision of material for thylakoid biosynthesis, photosystems assembly) and the loss of the plastid division machinery. Altogether, the data provide new insights on the chromoplast differentiation process while enriching our knowledge of the plant plastid proteome.
Published: 2012
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40. Clinical features and sex hormone profile in male patients with frontal fibrosing alopecia: A multicenter retrospective study with 33 patients

Author: Jorge Larrondo, Paulo Müller Ramos, Marina Mattos Rebeis, Isabella Doche, Maria Cecília Rivitti-Machado, Marcello Menta Simonsen Nico, Antonella Tosti, Paula Gerlero, Felipe Mardones, Violeta Duarte Tortelly, Daniel Fernandes Melo, Martha L. González, Universidade de São Paulo (USP), University of State of Rio de Janeiro, Universidade Estadual Paulista (UNESP), Universidad del Desarollo, Clínica Universidad de los Andes, Universidad ICESI, and Miller School of Medicine
Subjects: Male, medicine.medical_specialty, biology, business.industry, Frontal fibrosing alopecia, Lichen Planus, Alopecia, Retrospective cohort study, Frontal vein, Dermatology, Scarring alopecia, medicine.disease, Fibrosis, Sex hormone-binding globulin, medicine.vein, Male patient, medicine, biology.protein, Humans, Gonadal Steroid Hormones, Facial papules, business, Retrospective Studies, Hormone
Abstract: Made available in DSpace on 2022-04-29T08:29:31Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Departament of Dermatology University of São Paulo Medical School Department of Dermatology University of State of Rio de Janeiro Department of Dermatology Universidade Estadual Paulista Department of Dermatology Clínica Alemana Universidad del Desarollo Department of Dermatology Clínica Universidad de los Andes Department of Dermatology Fundación Valle del Lili Hospital Universitario Universidad ICESI Department of Dermatology and Cutaneous Surgery University of Miami Miller School of Medicine Department of Dermatology Universidade Estadual Paulista
Published: 2022
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41. A CCR4 antagonist combined with vaccines induces antigen-specific CD8+ T cells and tumor immunity against self antigens

Author: Frédéric Batteux, Eric Tartour, Françoise Quintin-Colonna, Christopher Chiu, Brad H. Nelson, Patrice Ravel, Corinne Tanchot, Douglas Hanahan, Alain Gey, Wolf H. Fridman, Ludger Johannes, Yves Montier, Federico Sandoval, Sylvie Garcia, Estelle Dransart, Luís Carlos de Souza Ferreira, Jagadeesh Bayry, Olivier Adotevi, Hélène Péré, Cécile Badoual, Elie Marcheteau, Nathalie Merillon, Yu-Chun Lone, Paris-Centre de Recherche Cardiovasculaire (PARCC - UMR-S U970), Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre de Recherche des Cordeliers (CRC (UMR_S 872)), Université Pierre et Marie Curie - Paris 6 (UPMC)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire - Alfort (ENVA), Laboratoire Trafic, Signalisation et Ciblage Intracellulaires, Institut Curie [Paris], Service d'anatomo-pathologie [CHU HEGP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Service d'immunologie [HEGP, Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpitaux Universitaires Paris Ouest - Hôpitaux Universitaires Île de France Ouest (HUPO), Centre de Biochimie Structurale [Montpellier] (CBS), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Laboratoire d'immunologie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Hôpital Cochin [AP-HP], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-EA1833, Diabetes center, University of California [San Francisco] (UCSF), University of California-University of California, Biologie des Populations Lymphocytaires, Institut Pasteur [Paris]-Centre National de la Recherche Scientifique (CNRS), Greffes d'Epitheliums et Regulation de l'Activation Lymphocytaire, Université Paris-Sud - Paris 11 (UP11)-Institut National de la Santé et de la Recherche Médicale (INSERM), Department of Microbiology, Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP)-University of São Paulo (USP), Trev and Joyce Deeley Research Center, Vancouver Island Center, This work was supported by Canceropole Ile de France, ANR (Agence Nationale de la Recherche), Ligue contre le Cancer, Association pour la Recherche sur le Cancer, Pole de compétitivité Medicen (Immucan) Center d'investigation Clinique en Biothérapie (CIC-BT505), Labex Immuno-Oncology, Institut National du Cancer (INCA), European Community's Seventh Framework Program (FP7/2007-2013, under grant agreement no. 260338ALLFUN, J.B)., European Project: 260338,EC:FP7:HEALTH,FP7-HEALTH-2010-single-stage,ALLFUN(2010), Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Hôpital Européen Georges Pompidou [APHP] (HEGP), Université Paris Descartes - Paris 5 (UPD5)-Université Pierre et Marie Curie - Paris 6 (UPMC)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), École nationale vétérinaire d'Alfort (ENVA), Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre National de la Recherche Scientifique (CNRS)-Institut Pasteur [Paris], University of California [San Francisco] (UC San Francisco), University of California (UC)-University of California (UC), Institut Pasteur [Paris] (IP)-Centre National de la Recherche Scientifique (CNRS), Universidade de São Paulo = University of São Paulo (USP)-Institute of Biomedical Sciences (ICB/USP), and Universidade de São Paulo = University of São Paulo (USP)
Subjects: MESH: Combined Modality Therapy, MESH: Antigens, Neoplasm, MESH: Receptors, CCR4, T-Cell Antigen Receptor Specificity, CD8-Positive T-Lymphocytes, Biochemistry, Autoantigens, MESH: Cancer Vaccines, Mice, 0302 clinical medicine, Neoplasms, CCL17, Cytotoxic T cell, MESH: Animals, MESH: Neoplasms, 0303 health sciences, education.field_of_study, MESH: Immunologic Factors, Peripheral tolerance, hemic and immune systems, Hematology, Combined Modality Therapy, MESH: CD8-Positive T-Lymphocytes, 3. Good health, MESH: T-Cell Antigen Receptor Specificity, MESH: Autoantigens, 030220 oncology & carcinogenesis, MESH: Tumor Escape, [SDV.IMM]Life Sciences [q-bio]/Immunology, Female, Receptors, CCR4, MESH: Mice, Transgenic, Immunology, Population, Antineoplastic Agents, Mice, Transgenic, chemical and pharmacologic phenomena, Biology, Cancer Vaccines, 03 medical and health sciences, Immune system, Antigen, Antigens, Neoplasm, MESH: Mice, Inbred C57BL, Animals, Humans, Immunologic Factors, education, MESH: Mice, 030304 developmental biology, MESH: Humans, MICROBIOLOGIA, Cell Biology, Mice, Inbred C57BL, Disease Models, Animal, MESH: Antineoplastic Agents, Tumor Escape, Cancer vaccine, MESH: Disease Models, Animal, MESH: Female, CD8
Abstract: Regulatory T cells (Tregs) may impede cancer vaccine efficacy in hematologic malignancies and cancer. CCR4 antagonists, an emergent class of Treg inhibitor, have been shown to block recruitment of Tregs mediated by CCL22 and CCL17. Our aim was to demonstrate the ability of a CCR4 antagonist (a small chemical molecule identified in silico) when combined with vaccines to break peripheral tolerance controlled by Tregs, a prerequisite for the induction of CD8+ T cells against self Ags. Immunization of transgenic or normal mice expressing tumor-associated self Ags (Her2/neu, OVA, gp100) with a CCR4 antagonist combined with various vaccines led to the induction of effector CD8+ T cells and partial inhibition of tumor growth expressing self Ags in both prophylactic and therapeutic settings. The CCR4 antagonist was more efficient than cyclophosphamide to elicit anti-self CD8+ T cells. We also showed that the population of Tregs expressing CCR4 corresponded to memory (CD44high) and activated (ICOS+) Tregs, an important population to be targeted to modulate Treg activity. CCR4 antagonist represents a competitive class of Treg inhibitor able to induce functional anti-self CD8+ T cells and tumor growth inhibition when combined with vaccines. High expression of CCR4 on human Tregs also supports the clinical development of this strategy.
Published: 2011
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42. Metabolic and molecular events occurring during chromoplast biogenesis

Author: Cristina Barsan, Eduardo Purgatto, Isabel Egea, Christian Chervin, Wanping Bian, Alain Latché, Jean-Claude Pech, Mohamed Zouine, Mondher Bouzayen, Institut National Polytechnique de Toulouse - INPT (FRANCE), Institut National de la Recherche Agronomique - INRA (FRANCE), Universidade de São Paulo - USP (BRAZIL), Génomique et Biotechnologie des Fruits (GBF), Institut National de la Recherche Agronomique (INRA)-École nationale supérieure agronomique de Toulouse [ENSAT]-Institut National Polytechnique (Toulouse) (Toulouse INP), Université Fédérale Toulouse Midi-Pyrénées-Université Fédérale Toulouse Midi-Pyrénées, Université Fédérale Toulouse Midi-Pyrénées, Universidade de São Paulo (USP), University of Chongqing (China), French Embassy in Bucharest (Romania), 'Fundación Séneca' (Murcia, Spain)', Government of Brazil (CNPq), Midi-Pyrénées Regional Council, Bian, Wanping, Barsan, Cristina, Pech, Jean-Claude, and Institut National Polytechnique de Toulouse - Toulouse INP (FRANCE)
Subjects: 0106 biological sciences, Proteomics, [SDV]Life Sciences [q-bio], Carotenoids biosynthetis, Plant Science, Biology, Pentose phosphate pathway, 01 natural sciences, 03 medical and health sciences, lcsh:Botany, Chromoplast, Chromoplasts, Génétique des plantes, BIOQUÍMICA DE ALIMENTOS, Plastid, Gene, Carotenoid, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Chlorophyll biosynthesis, Calvin cycle, Plastoglobules, Fruit ripening, Chromoplasts – Proteomics – Carotenoids biosynthetis – Chlorophyll biosynthesis – Chlorophyll degradation – Calvin cycle – Plastoglobules – Fruit ripening, food and beverages, lcsh:QK1-989, Chloroplast, Botanique, Biochemistry, chemistry, Biogenesis, 010606 plant biology & botany
Abstract: Chromoplasts are nonphotosynthetic plastids that accumulate carotenoids. They derive from other plastid forms, mostly chloroplasts. The biochemical events responsible for the interconversion of one plastid form into another are poorly documented. However, thanks to transcriptomics and proteomics approaches, novel information is now available. Data of proteomic and biochemical analysis revealed the importance of lipid metabolism and carotenoids biosynthetic activities. The loss of photosynthetic activity was associated with the absence of the chlorophyll biosynthesis branch and the presence of proteins involved in chlorophyll degradation. Surprisingly, the entire set of Calvin cycle and of the oxidative pentose phosphate pathway persisted after the transition from chloroplast to chromoplast. The role of plastoglobules in the formation and organisation of carotenoid-containing structures and that of theOrgene in the control of chromoplastogenesis are reviewed. Finally, using transcriptomic data, an overview is given the expression pattern of a number of genes encoding plastid-located proteins during tomato fruit ripening.
Published: 2011
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43. Intra- and intercompartmental movement of gammadelta T cells: intestinal intraepithelial and peripheral gammadelta T cells represent exclusive nonoverlapping populations with distinct migration characteristics

Author: Jan D. Haas, Vijaykumar Chennupati, Frano H. Malinarich, Susanne Schmitz, Reinhold Förster, Tim Worbs, Bernard Malissen, Immo Prinz, Xiaosun Liu, Hannover Medical School [Hannover] (MHH), Faculty of Medicine, University of Chile (UCHILE), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), Centre d'Immunologie de Marseille - Luminy (CIML), Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), and Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)
Subjects: Adoptive cell transfer, T-Lymphocytes, MESH: Flow Cytometry, MESH: Lymph Nodes, Cell Separation, MESH: T-Lymphocyte Subsets, MESH: Mice, Knockout, Mice, 0302 clinical medicine, Cell Movement, T-Lymphocyte Subsets, Immunology and Allergy, MESH: Microscopy, Confocal, MESH: Animals, Intestinal Mucosa, MESH: Cell Movement, Mice, Knockout, 0303 health sciences, Microscopy, Confocal, Receptors, Antigen, T-Cell, gamma-delta, Flow Cytometry, Intestinal epithelium, Adoptive Transfer, Peripheral, MESH: Intestinal Mucosa, [SDV.IMM]Life Sciences [q-bio]/Immunology, Immunology, Biology, MESH: Cell Separation, 03 medical and health sciences, MESH: Mice, Inbred C57BL, MESH: Receptors, Antigen, T-Cell, gamma-delta, Animals, Cell Lineage, Immunity, Mucosal, MESH: Mice, 030304 developmental biology, T-cell receptor, MESH: Cell Lineage, Transplantation, Mice, Inbred C57BL, MESH: Adoptive Transfer, MESH: T-Lymphocytes, MESH: Immunity, Mucosal, Intraepithelial lymphocyte, Lymph Nodes, Peripheral lymph, 030215 immunology, Homing (hematopoietic)
Abstract: Unlike the ∼1% of γδ TCR-positive T cells being regularly present in blood and secondary lymphoid organs (peripheral γδ T cells), ∼50–60% of small intestinal intraepithelial lymphocytes (iIELs) in the mouse express the γδ TCR (γδ iIELs). In this study, we investigated the overlap and exchange of γδ iIELs and γδ T cells found in peripheral secondary lymphoid organs. Using two-photon laser-scanning microscopy, we found γδ T cells within peripheral lymph nodes to be highly motile, whereas γδ iIELs were characterized by a locally confined scanning behavior. Our results implied a strict separation of peripheral γδ T cells and γδ iIELs. Nevertheless, γδ iIELs could be efficiently regenerated from bone marrow-derived precursors in irradiated or T cell-deficient adult mice. However, outside the intestinal epithelium, survival of γδ iIELs was very poor. In CCR9-deficient mice, homing of γδ iIELs was impaired, but did not lead to an accumulation of γδ iIEL-like cells in the periphery. Conversely, in situations in which specific γδ iIEL niches were empty, adoptive transfer of isolated γδ iIELs led to a sustained engraftment of transferred γδ iIELs in the intestinal epithelium for at least 100 d. Furthermore, we demonstrated by heterotopic intestinal transplantation experiments that an exchange of γδ iIELs only rarely happens in the steady state of adult mice. We therefore conclude that peripheral versus intestinal intraepithelial γδ T cells are exclusive, nonoverlapping populations that virtually do not exchange with each other.
Published: 2010
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44. Revisiting Human IL-12R beta 1 Deficiency A Survey of 141 Patients From 30 Countries

Author: Chris Nieuwhof, Yu-Lung Lau, Klara Frecerova, Suzanne T. Anderson, Vas Novelli, Dinakantha S. Kumararatne, Figen Dogu, Matías Oleastro, Barbara Pietrucha, Melike Keser, Xi-qiang Yang, Anete Sevciovic Grumach, Renate Blüetters-Sawatzki, Necil Kutukculer, Lucile Janniere, Sigifredo Pedraza, María Isabel Gaillard, Isabel Caragol, Ruth Aldana, Parisa Adimi, Orchidée Filipe-Santos, Liliane Schandené, Ludovic de Beaucoudrey, Alejandra King, Rainer Doffinger, Sergio D. Rosenzweig, Dewton Moraes-Vasconcelos, Guzide Aksu, Pamela Pui Wah Lee, Abdulaziz Al-Ghonaium, Aurélie Cobat, David Tuerlinckx, Michael Levin, Mohammed Bejaoui, Nevin Hatipoğlu, Kinda Schepers, Francisco J. Espinosa-Rosales, Andrea Bernasconi, Aydan Ikinciogullari, Jacob Levy, Mohammad S. Ehlayel, Małgorzata Pac, Darko Richter, David A. Lammas, David B. Lewis, Janine Reichenbach, Gonul Tanir, Joachim Freihorst, Tuba Turul Ozgur, Sulaiman Al-Ajaji, Masao Matsuoka, Jacqueline Feinberg, Smita Y. Patel, Abdullah A. Alangari, Judith Yancoski, Andrew Exley, Saleh Al-Muhsen, Ridha Barbouche, Tatsunori Sakai, Ana Codoceo, Xiao-Fei Kong, Claire-Anne Siegrist, Jamila El Baghdadi, Bernhard Fleckenstein, Xiaochuan Wang, Sami Al-Hajjar, Ingrid Müller-Fleckenstein, Capucine Picard, Gabriela Castro, Jean-Laurent Casanova, Tong-Xin Chen, Frans De Baets, Namik Ozbek, Alain Fischer, Liliana Bezrodnik, Aileen C. Cohen, Ariane Chapgier, Olle Jeppsson, Imen Ben-Mustapha, Saniye Gülle, Revathi Raj, Françoise Mascart, Arina Samarina, Davood Mansouri, Nima Parvaneh, Yoann Rose, Koon Wing Chan, Yuanyuan Xie, Alberto José da Silva Duarte, Ahmed Aziz Bousfiha, Claire Fieschi, Steven M. Holland, Meteran Ozen, Walther H. Haas, Stéphanie Boisson-Dupuis, Carlos Rodríguez-Gallego, Li-Ping Jiang, Ayper Somer, Suna Asilsoy, André Efira, Yaryna Boyko, Laurent Abel, Setareh Mamishi, Xiaohong Wang, Ulrich Baumann, Filomeen Haerynck, Zobaida Alsum, Kuender D Yang, Jacinta Bustamante, Daniela Di Giovani, Ozden Sanal, Maylis de Suremain, Yildiz Camcioglu, Christiane Vermylen, Ben-Zion Garty, Jutta Bernhöft, Ivelisse Natera, Suliman Al-Jumaah, Chaomin Zhu, David Nadal, Husn H. Frayha, Cigdem Aydogmus, Génétique Humaine des Maladies Infectieuses (Inserm U980), Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Paris Descartes - Paris 5 (UPD5), Université Paris Descartes - Faculté de Médecine (UPD5 Médecine), Université Paris Descartes - Paris 5 (UPD5), St. Giles Laboratory of Human Genetics of Infectious Diseases, The Rockefeller University, Department of Pediatrics, King Faysal Hospital and Research center, Coll Med, Prince Naif Ctr Immunol Res, King Saud University [Riyadh] (KSU), Coll Med, Dept Pediat, IFR Necker-Enfants Malades (IRNEM), Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Developpement Normal et Pathologique du Système Immunitaire, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance Publique-Hôpitaux de Paris, Service d'Immunologie et d'Hématologie Pédiatrique, Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Sami Ulus Children Hlth & Dis Training & Res Ctr, Ankara, Dept Pediat, King Fahad Natl Guard Hosp, Shahid Beheshti University of Medical Sciences, Laboratoire d'immunologie clinique [Institut Pasteur de Tunis], Institut Pasteur de Tunis, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Dept Immunol, Juan Pedro Garrahan Natl Hosp Pediat, Schneider Childrens Med Ctr Israel, Hospital Universitario de Gran Canaria Dr Negrin, Immunol Lab, Vall d'Hebron University Hospital [Barcelona], Ege University, Dept Clin Biochem & Immunol, Cambridge University Hospitals NHS Foundation Trust, Addenbrookes Hospital, Inflammat & Immunol Unit, Dept Pathol, Papworth Hosp NHS Fdn Trust, Inflammat & Immunol Unit, Dept Thorac Med, Karolinska University Hospital [Stockholm], Univ Childrens Hosp Zurich, Dept Infect Dis, Lviv Reg Specialized Childrens Hosp, St Marys Hosp, Imperial Coll Sch Med, Childrens Mem Hlth Inst, Immunobiol Clin, Hop Erasme, Université Libre de Bruxelles [Bruxelles] (ULB), Immunodeficiency Dept, Hop Erasme, Lab Vaccinol & Mucosal Immun, Dept Internal Med, Natl Hosp Org Kumamoto Med Ctr, Laboratory of Virus Control, Kyoto University [Kyoto], University of Geneva [Switzerland], Dept Int Relat, Minist Hlth Slovaquie, Dept Pediat Hematol & Oncol, Dept Pediat Pulmonol & Neonatol, Hannover Medical School [Hannover] (MHH), Univ Hosp Ctr Rebro, Dept Pediat Pulmonol & Immunol, Ghent University Hospital, Infect Dis Unit, Great Ormond St Hosp Sick Children, Ctr Immune Regulat, Univ Birmingham, Sch Med, MRC, Cliniques Universitaires Saint-Luc [Bruxelles], Dept Pediat, Mt Godinne Clin, Catholic University Louvain, Dept Internal Med, Div Clin & Expt Immunol, Maastricht University [Maastricht], Dept Infect Dis Epidemiol, Unit Resp Infect, Robert Koch Institute [Berlin] (RKI), Univ Erlangen Nurnberg, Pediatric Department, Ben-Gurion University of the Negev (BGU), Stanford University [Stanford], Lab Clin Infect Dis, NIAID, TMRC, NIH, Bethesda, Dept Pediat Allergy, Chang Gung Childrens Hosp, Dept Clin Immunol, Fudan University [Shanghai], Clin Immunol Lab, Chongqing Medical University, Dept Paediat & Adolescent Med, The University of Hong Kong (HKU), Universidade Federal da Bahia (UFBA), Dept Pediat,Sch Med, Shanghai Jiao Tong University [Shanghai], Univ Hosp Caracas, Hosp Ninos Luis Calvo Mackenna, Childrens Hosp Ricardo Gutierrez Buenos Aires, Dept Dermatol, Lab Invest Dermatol & Immunodeficiencies, Universidade de São Paulo (USP), Dept Pulmonol, Childrens Hosp Federico Gomez, Natl Inst Pediat, Immunodeficiencies Res Unit, Mexico City, DF, Mexico, Natl Ctr Bone Marrow Graft, Tunis, Clinical Immunology Unit, Ibn Rochd Hospital King Hassan II University-Aïn Chok, Genetics Unit, Military Hospital Mohamed V, Baskent universitesi, Dept Pediat Infect Dis & Clin Immunol, Istanbul Univ, Dept Pediat Infect Dis & Immunol, Bakirkoy Matern & Childrens State Hosp, Dr Behcet Uz Children Res & Training Hosp, Department of Pediatrics, Infectious Diseases, Cerrahpacsa Medical School, Hacettepe University = Hacettepe Üniversitesi, Fac Med Malatya, Tehran University of Medical Sciences (TUMS), Dept Biochem, Minist Hlth Mexico, Department of Pediatric and Adolescent Medicine, Hamad medical corporation, Laboratoire d'immunologie, Groupe Hospitalier Saint Louis - Lariboisière - Fernand Widal [Paris], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-Assistance publique - Hôpitaux de Paris (AP-HP) (APHP), Service d'immuno-hématologie pédiatrique [CHU Necker], Assistance publique - Hôpitaux de Paris (AP-HP) (APHP)-CHU Necker - Enfants Malades [AP-HP], Çocuk Sağlığı ve Hastalıkları, Ege Üniversitesi, Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM), Rockefeller University [New York], Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Université Paris Descartes - Paris 5 (UPD5)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Shahid Beheshti University of Medical Sciences [Tehran] (SBUMS), Shahid Beheshti University, Université libre de Bruxelles (ULB), Université Catholique de Louvain = Catholic University of Louvain (UCL), Friedrich-Alexander Universität Erlangen-Nürnberg (FAU), Stanford University, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Siegrist, Claire-Anne, Génétique Humaine des Maladies Infectieuses ( Inserm U980 ), Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Université Paris Descartes - Paris 5 ( UPD5 ), Université Paris Descartes - Faculté de Médecine ( UPD5 Médecine ), Université Paris Descartes - Paris 5 ( UPD5 ), King Saud University [Riyadh] ( KSU ), IFR Necker-Enfants Malades ( IRNEM ), Assistance publique - Hôpitaux de Paris (AP-HP)-Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Université Paris Descartes - Paris 5 ( UPD5 ) -Institut National de la Santé et de la Recherche Médicale ( INSERM ) -Centre National de la Recherche Scientifique ( CNRS ), Assistance publique - Hôpitaux de Paris (AP-HP), Institut Pasteur de Tunis-Réseau International des Instituts Pasteur ( RIIP ), Université Libre de Bruxelles [Bruxelles] ( ULB ), Hannover Medical School [Hannover] ( MHH ), Robert Koch Institute [Berlin] ( RKI ), Ben-Gurion University of the Negev ( BGU ), The University of Hong Kong ( HKU ), Universidade Federal da Bahia ( UFBA ), Universidade de São Paulo ( USP ), Tehran University of Medical Sciences, Assistance publique - Hôpitaux de Paris (AP-HP)-CHU Necker - Enfants Malades [AP-HP], Interne Geneeskunde, RS: CARIM School for Cardiovascular Diseases, UCL - SSS/IREC/PEDI - Pôle de Pédiatrie, UCL - (SLuc) Service d'hématologie et d'oncologie pédiatrique, and UCL - (MGD) Service de pédiatrie
Subjects: Male, Proband, [SDV]Life Sciences [q-bio], Mycobacterium tuberculosis/isolation & purification, Mycobacterium bovis/isolation & purification, Disease, Interleukin-12 Receptor beta 1 Subunit - deficiency, genetics, Cytokines - blood, 0302 clinical medicine, Mycobacterium bovis - isolation & purification, Child, 0303 health sciences, ddc:618, Interleukin-12 Receptor beta 1 Subunit/*deficiency/genetics, Nontuberculous Mycobacteria/isolation & purification, biology, Age Factors, Nontuberculous Mycobacteria, Mycobacterium Infections, Nontuberculous/epidemiology/genetics, General Medicine, Middle Aged, Mycobacterium bovis, Penetrance, Mycobacteria, Atypical - isolation & purification, 3. Good health, Child, Preschool, Cytokines, Female, medicine.symptom, Adult, medicine.medical_specialty, Tuberculosis, Adolescent, Genotype, Mycobacterium Infections, Nontuberculous, Mycobacterium tuberculosis - isolation & purification, Cytokines/blood, Asymptomatic, Article, Mycobacterium tuberculosis, 03 medical and health sciences, Internal medicine, General & Internal Medicine, Interleukin-12 Receptor beta 1 Subunit, medicine, Humans, Survival analysis, 030304 developmental biology, Mycobacterium Infections, Atypical - epidemiology, genetics, [ SDV ] Life Sciences [q-bio], business.industry, Infant, Newborn, Infant, biology.organism_classification, medicine.disease, Survival Analysis, Immunology, Nontuberculous mycobacteria, business, 030215 immunology
Abstract: WOS: 000283840300002, PubMed ID: 21057261, Interleukin-12 receptor beta 1 (IL-12R beta 1) deficiency is the most common form of Mendelian susceptibility to mycobacterial disease (MSMD). We undertook an international survey of 141 patients from 102 kindreds in 30 countries. Among 102 probands, the first infection occurred at a mean age of 2.4 years. In 78 patients, this infection was caused by Bacille Calmette-Guerin (BCG; n = 65), environmental mycobacteria (EM; also known as atypical or nontuberculous mycobacteria) (n = 9) or Mycobacterium tuberculosis (n = 4). Twenty-two of the remaining 24 probands initially presented with nontyphoidal, extraintestinal salmonellosis. Twenty of the 29 genetically affected sibs displayed clinical signs (69%); however 8 remained asymptomatic (27%). Nine nongenotyped sibs with symptoms died. Recurrent BCG infection was diagnosed in 15 cases, recurrent EM in 3 cases, recurrent salmonellosis in 22 patients. Ninety of the 132 symptomatic patients had infections with a single microorganism. Multiple infections were diagnosed in 40 cases, with combined mycobacteriosis and salmonellosis in 36 individuals. BCG disease strongly protected against subsequent EM disease (p = 0.00008). Various other infectious diseases occurred, albeit each rarely, yet candidiasis was reported in 33 of the patients (23%). Ninety-nine patients (70%) survived, with a mean age at last follow-up visit of 12.7 years +/- 9.8 years (range, 0.5-46.4 yr). IL-12R beta 1 deficiency is characterized by childhood-onset mycobacteriosis and salmonellosis, rare recurrences of mycobacterial disease, and more frequent recurrence of salmonellosis. The condition has higher clinical penetrance, broader susceptibility to infections, and less favorable outcome than previously thought., Rockefeller University Center for Clinical and Translational Science [5UL1RR024143-03]; Rockefeller University; ANRFrench National Research Agency (ANR); PHRC; EUEuropean Union (EU) [LHSP-CT-2005-018736]; BNP Paribas Foundation; Dana Foundation; March of DimesMarch of Dimes; Fondation pour la Recherche MedicaleFondation pour la Recherche Medicale; Howard Hughes Medical InstituteHoward Hughes Medical Institute, The St. Giles Laboratory of Human Genetics of Infectious Diseases is supported by grants from The Rockefeller University Center for Clinical and Translational Science grant number 5UL1RR024143-03, and The Rockefeller University, ANR, PHRC, EU (LHSP-CT-2005-018736), BNP Paribas Foundation, the Dana Foundation, and the March of Dimes. LDB is supported by the Fondation pour la Recherche Medicale as part of the PhD program of Pierre et Marie Curie University (Paris, France). JLC was an International Scholar of the Howard Hughes Medical Institute.
Published: 2010
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45. Constant tcr triggering suggests that the tcr expressed on intestinal intraepithelial gamma delta t cells is functional in vivo

Author: Bernard Malissen, Frano H. Malinarich, Rodrigo Quera, Elena Grabski, Susanne Schmitz, Jan D. Haas, Immo Prinz, Reinhold Förster, Enzo Candia, Marcela A. Hermoso, Tim Worbs, Vijaykumar Chennupati, Hannover Medical School [Hannover] (MHH), Faculty of Medicine, University of Chile (UCHILE), Institute of Biomedical Sciences (ICB/USP), Universidade de São Paulo = University of São Paulo (USP)-Universidade de São Paulo = University of São Paulo (USP), Centre for Experimental and Clinical Infection Research (TWINCORE), Helmholtz Centre for Infection Research (HZI)-Medizinische Hochschule Hannover (MHH), Las Condes Clinic (CLC), Gastroenterology Unit, Centre d'Immunologie de Marseille - Luminy (CIML), Aix Marseille Université (AMU)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Universidade de São Paulo (USP)-Universidade de São Paulo (USP), and Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Aix Marseille Université (AMU)
Subjects: Antigens, Differentiation, T-Lymphocyte, T-Lymphocytes, Lymphocyte Activation, Mice, 0302 clinical medicine, Immunology and Allergy, MESH: Animals, Intestinal Mucosa, Chemokine CCL4, Receptor, MESH: Antigens, CD, Cells, Cultured, 0303 health sciences, CD69, Pattern recognition receptor, Receptors, Antigen, T-Cell, gamma-delta, Cell biology, medicine.anatomical_structure, MESH: Intestinal Mucosa, [SDV.IMM]Life Sciences [q-bio]/Immunology, MESH: Cells, Cultured, MESH: Mice, Transgenic, MESH: Interferon-gamma, CD8 Antigens, T cell, Immunology, Mice, Transgenic, Biology, MESH: Calcium Signaling, Interferon-gamma, 03 medical and health sciences, Antigen, Antigens, CD, MESH: Mice, Inbred C57BL, MESH: Receptors, Antigen, T-Cell, gamma-delta, MESH: Antibodies, Blocking, medicine, Animals, Lectins, C-Type, Calcium Signaling, MESH: Chemokine CCL4, Antibodies, Blocking, MESH: Lymphocyte Activation, MESH: Mice, 030304 developmental biology, T-cell receptor, In vitro, Mice, Inbred C57BL, MESH: T-Lymphocytes, MESH: Antigens, Differentiation, T-Lymphocyte, Intraepithelial lymphocyte, MESH: Antigens, CD8, MESH: Lectins, C-Type, 030215 immunology
Abstract: International audience; Intestinal intraepithelial lymphocytes carrying the γδ TCR (γδ iIEL) are involved in the maintenance of epithelial integrity. γδ iIEL have an activated phenotype, characterized by CD69 expression and increased cell size compared with systemic T lymphocytes. As an additional activation marker, the majority of γδ iIEL express the CD8αα homodimer. However, our knowledge about cognate ligands for most γδ TCR remains fragmentary and recent advances show that γδ T cells including iIEL may be directly activated by cytokines or through NK-receptors, TLR and other pattern recognition receptors. We therefore asked whether the TCR of γδ iIEL was functional beyond its role during thymic selection. Using TcrdH2BeGFP (Tcrd, T-cell receptor δ locus; H2B, histone 2B) reporter mice to identify γδ T cells, we measured their intracellular free calcium concentration in response to TCR-crosslinking. In contrast to systemic γδ T cells, CD8αα(+) γδ iIEL showed high basal calcium levels and were refractory to TCR-dependent calcium-flux induction; however, they readily produced CC chemokine ligand 4 (CCL4) and IFN-γ upon TCR triggering in vitro. Notably, in vivo blocking of the γδ TCR with specific mAb led to a decrease of basal calcium levels in CD8αα(+) γδ iIEL. This suggests that the γδ TCR of CD8αα(+) γδ iIEL is constantly being triggered and therefore functional in vivo.
Published: 2010
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46. Nutrient availability affected by silicate and Azospirillum brasilense application in corn–wheat rotation

Author: Antônio Leonardo Campos Biagini, Paulo H. Pagliari, Marcelo Carvalho Minhoto Teixeira Filho, Fernando Shintate Galindo, Salatiér Buzetti, Guilherme Carlos Fernandes, Renan Francisco Rimoldi Tavanti, Willian Lima Rodrigues, Universidade de São Paulo (USP), Southwest Research and Outreach Center, and Universidade Estadual Paulista (UNESP)
Subjects: chemistry.chemical_compound, Nutrient, chemistry, Agronomy, Biology, Azospirillum brasilense, Rotation, biology.organism_classification, Agronomy and Crop Science, Silicate
Abstract: Made available in DSpace on 2022-05-01T08:44:44Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The interactive effectof silicon (Si) application on soil fertility is poorly understood when inoculation with Azospirillum brasilense is performed. This research determined if Si application and A. brasilense inoculation to corn (Zea mays L.) and wheat (Triticum aestivum L.) has agronomic benefits on soil nutrient concentration and grain yield. Treatments were tested under a no-till system in a Rhodic Haplustox in a full factorial design with four replicates and comprised (a) two soil amendment sources—calcium (Ca) and magnesium (Mg) silicate and dolomitic limestone; (b) with and without seed inoculation with A. brasilense; and (c) five sampling dates collected during two corn–wheat crop rotations (2015–2017). Calcium and Mg silicate application was effective at decreasing soil hydrogen + aluminum (H+Al) and Al content and increasing soil pH, sum of bases, and base saturation. However, silicate changed soil nutrient availability, reduced cation exchange capacity, phosphorus (P) and Mg content and did not significantly affect (p >.05) corn and wheat grain yield. Inoculation reduced Al toxicity, with lower H+Al and Al contents, and increased K content, leading to a greater pH and base saturation after four cropping seasons. Inoculation increased corn and wheat grain yield by 8.2 and 8.3%, respectively. Application of Ca and Mg silicate was more effective than limestone as a liming agent but could result in decreased availability of certain nutrients in soil. Inoculation with A. brasilense could be an important management practice to maintain adequate Si content in soil when limestone is applied. However, with silicate application, A. brasilense could increase Si depletion in soil. Center for Nuclear Energy in Agriculture Univ. of São Paulo Dep. of Soil Water and Climate Univ. of Minnesota Southwest Research and Outreach Center Dep. of Plant Health Rural Engineering and Soils São Paulo State Univ. Dep. of Plant Health Rural Engineering and Soils São Paulo State Univ.
Published: 2021
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47. Sperm viability, serological, molecular, and modified seminal plasma agglutination tests in the diagnosis of Leptospira in the semen and serum of bovine bulls

Author: Geraldo de Nardi Junior, Antonio Francisco de Souza Filho, Rogério Giuffrida, Gustavo Henrique Batista Lara, Marcelo George Mungai Chacur, Lorrayne de Souza Araújo Martins, Adriana Cortez, Fabio Morato Monteiro, Joeleni Rosa dos Santos, Sérgio Ricardo Maiolino, Rodrigo Garcia Motta, Márcio Garcia Ribeiro, Helio Langoni, Thaís Spessotto Bello, Marcos Bryan Heinemann, Universidade Estadual Paulista (UNESP), Santo Amaro University - UNISA, University of Oeste Paulista, Technology Faculty - FATEC, Institute of Animal Science, and Universidade de São Paulo (USP)
Subjects: Male, Serum, endocrine system, animal diseases, Seroprevalence, Semen, Biology, Microbiology, Serology, Andrology, Blood serum, Agglutination Tests, RNA, Ribosomal, 16S, Direct agglutination test, Media Technology, medicine, Animals, Leptospirosis, Reproduction by natural means, Leptospira, Azoospermia, SÊMEN ANIMAL, urogenital system, Veterinary Microbiology - Research Paper, medicine.disease, Spermatozoa, Sperm, Agglutination (biology), Bull leptospirosis, Necrospermia, Cattle, Molecular diagnosis
Abstract: Made available in DSpace on 2022-05-01T08:44:43Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 The present study investigated the serum microscopic agglutination test (MAT) among 203 bovine bulls with reproduction by natural means, without apparent signs of orchitis or inflammation of accessory reproductive glands. Simultaneously, the semen of all bulls was subjected to sperm viability analysis and PCR based on the 16S rRNA gene. PCR-positive results of semen samples were confirmed by sequencing. A modified seminal plasma agglutination (MSPA) test, replacing the blood serum of all bulls in the MAT with seminal plasma was performed as well. Eight (8/203 = 3.9%) semen samples from bulls were considered nonviable (necrospermia and azoospermia) without relation to the PCR diagnosis. No agglutinin titers were identified in MSPA test. A high frequency (132/203 = 65%) of leptospiral agglutinin titers was identified in the MAT, particularly for the Sejroe serogroup (Hardjo CTG, 100/203 = 49.3%; Wolffi 74/203 = 36.4%; Guaricura 72/203 = 35.5%; and Hardjoprajitno 56/203 = 27.6%). Three (3/203 = 1.5%) semen samples of bulls were positive in the PCR, but these results were not confirmed by sequencing. The high frequency of serovars from the Sejroe serogroup typically adapted to bovines indicates the need for measures for the prophylaxis/control of the pathogen on the sampled farms. Discrepancies among the MAT, sperm viability, and molecular detection of leptospires in semen highlight the need for a combination of methods to diagnose leptospirosis in bovine bulls. To our knowledge, modified seminal plasma agglutination is described for the first time here to investigate anti-Leptospira antibodies produced locally in the genital tract in the diagnosis of bovine leptospirosis among bulls that reproduce by natural means. Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP Santo Amaro University - UNISA Faculty of Veterinary Medicine University of Oeste Paulista Technology Faculty - FATEC APTA Beef Cattle Center Institute of Animal Science University of São Paulo - USP Department of Animal Production and Preventive Veterinary Medicine School of Veterinary Medicine an Animal Science São Paulo State University - UNESP
Published: 2021
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48. The attraction trick: males in early stages of disease become more chemically attractive to <scp>Nile</scp> tilapia ( <scp> Oreochromis niloticus </scp> ) females

Author: Vanessa Stramantinoli Rossi, Assaf Barki, Percilia Cardoso Giaquinto, Isabela Maria de Mello, Universidade de São Paulo (USP), and Agricultural Research Organization
Subjects: Male, media_common.quotation_subject, Foraging, Zoology, Context (language use), Olfaction, Aquatic Science, Biology, Fish Diseases, Nile tilapia, Animals, mate choice, Ecology, Evolution, Behavior and Systematics, media_common, fish, Reproduction, Cichlids, biology.organism_classification, Attraction, infection, Aeromonas hydrophila, chemical cues, Oreochromis, Mate choice, Female, Gram-Negative Bacterial Infections
Abstract: Made available in DSpace on 2022-04-28T19:43:33Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 In aquatic systems, olfaction plays an important role in acquiring information about the social environment and influences important behaviours in various contexts, including predator avoidance, foraging, aggressive and reproductive behaviour and mate selection. As the presence of diseases might modify individual odour, fish may use the variability in conspecifics’ odours as an indicator of the health status and infectious load of potential mates. Here, female Nile tilapia were tested for their ability to detect infected males and discriminate between bacterial infected and uninfected individuals by means of chemical cues. Females were allowed to choose between the odours of males infected by Aeromonas hydrophila bacteria and uninfected males. The findings show that female Nile tilapia initially showed a preference for infected males in terms of their first choice in a dichotomous choice test, but the total duration of time spent with the stimulus from infected males was not longer than that for the uninfected males. This may indicate that males at early stages of infection, i.e., without advanced clinical signs of infection, emit odours that allow them to enjoy the benefits of socialization when the infection is not yet detected by conspecifics. Thus, in the context of reproduction, males might attract female partners and have some chance of reproducing, before being avoided. Laboratory of Physiology and Animal Behaviour/Physiology Department State University of São Paulo Institute of Animal Science The Volcani Center Agricultural Research Organization
Published: 2021
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49. The potential for using metagreywacke to study metamorphism of amphibolite facies conditions: a comparison study within the Luminárias Nappe, Southern Brasília Orogen (Southeastern Brazil)

Author: George Luiz Luvizotto, Lara A. Patto, Renato de Moraes, Regiane Andrade Fumes, Universidade Estadual Paulista (UNESP), and Universidade de São Paulo (USP)
Subjects: Grossular, biology, Santo Antônio Unit, Metamorphic rock, Perple_X, Geochemistry, Metamorphism, Biotite schist, biology.organism_classification, Metagreywacke, XMapTools, Spessartine, Almandine, Pyrope, Geophysics, Geochemistry and Petrology, visual_art, visual_art.visual_art_medium, Protolith, Metamorphic facies, Geology, Carrancas Group, METAMORFISMO
Abstract: Made available in DSpace on 2022-04-28T19:43:23Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-10-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Metagreywacke is a very common rock type in orogenic settings. It occurs in several domains within the Neoproterozoic Southern Brasília Orogen, that registers convergent events related to the Western Gondwana formation. Studied rocks derive from the Luminárias Nappe, which is composed of metapelite (Campestre Unit), quartzite and metagreywacke (Santo Antônio Unit) that occur in the northern portion of the structure. The potential use of the metagreywacke in metamorphic studies and the metamorphic P–T path of the Santo Antônio Unit was constrained using isochemical phase diagram, quantitative compositional mapping, and composition mineral contours. The metamorphic peak mineral assemblage of the metagreywacke is biotite + plagioclase + garnet + muscovite + ilmenite + quartz. Rutile is a prograde phase, but is replaced by ilmenite at metamorphic peak, while chlorite is a retrograde phase, as it replaced biotite and garnet. The garnet is almandine rich, preserves zoning, with lower almandine and pyrope contents in the core and lower spessartine and grossular contents in the rim. The peak assemblage is stable at temperatures of 570 ˚C to 650 ˚C and pressures of 0.7 GPa to 1.05 GPa. Prograde conditions of 540 ˚C and 0.9 GPa are obtained using the garnet core spessartine composition and the rutile occurrence. The compositional mineral contours of garnet rim, combined with those of muscovite and plagioclase, intercept at 630 ˚C and 1.0 GPa and indicate peak metamorphic conditions. Later crystallization of chlorite took place during retrograde conditions at 580 ˚C and 0.7 GPa, defining a single clockwise P–T path. The calculated topology of metagreywacke pseudosection, in this study, resembles those of a typical metapelite, and the crystallization sequence of metamorphic index minerals, along a Barrovian gradient, with slightly distinguished mineral assemblages. The similarity and differences for the two rock types occurs, specially under amphibolite facies conditions. Therefore, the metagreywacke is a promising protolith for metamorphic studies, even if it presents marking differences from pelites. The constrained P–T path is similar to those presented in previous works carried out in the Luminárias Nappe, associated with the collisional setting in the Southern Brasília Orogen. Department of Geology São Paulo State University, Av. 24A Department of Mineralogy and Geotectonics University of São Paulo Rua do Lago Department of Geology São Paulo State University, Av. 24A CNPq: 141604/2018-2 FAPESP: 2015/05230-0 FAPESP: 2016/22627-3 CNPq: 311606/2019-9
Published: 2021
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50. Seed and seedling morphology of Bauhinia scandens L

Author: Cibele Chalita Martins, S A Basf, Givanildo Zildo da Silva, Francisco Guilhien Gomes Junior, Tatiane Sanches Jeromini, Lab Analyst Jr. on Basf S.A., UFJ, Universidade Estadual Paulista (UNESP), and Universidade de São Paulo (USP)
Subjects: Morphology (linguistics), Density, RAIOS X, food and beverages, Fabaceae, Biology, biology.organism_classification, Bauhinia scandens, X-ray, Seedling, Botany, Climbing bauhinia, Internal structure, General Agricultural and Biological Sciences
Abstract: Made available in DSpace on 2022-05-01T08:15:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Bauhinia scandens has potential importance as an ornamental and medicinal plant. Researchers have isolated and identified 1-O-alkylglycerol in the leaves of the B. scandens plant, and established antitumor properties using the Brine Shrimp toxicity test, an internationally accepted bioassay. Although this species has high potential, little is known about the viability of seedling production and the morphology of these plants, particularly in terms of seed characteristics and initial stages of germination. The objective of this study was to characterize the seed morphology, germination, and seedlings of B. scandens. Seed water content, weight, and coloration were evaluated. This study also included a description of seed biometrics, external and internal structures, germination, and seedling morphology. Internal seed morphology was evaluated by the anatomical sectioning and X-ray methods. The morphology data obtained were subjected to descriptive statistical analysis and germination data were determined using Cramér's V. B. scandens seeds have a coloration ranging from very dark grayish-red to dark reddish-brown, flat oblong shapes, and rounded bases and apexes with full or slightly undulating margins. Healthy seedlings are produced mainly by seeds with well-formed internal structures. The reddish petiole of the seedling leaves is a taxonomic character for B. scandens identification. The non-domestication and genetic variability of this species reflect on the seed and seedling color and size variation. Lab Analyst Jr. on Basf S.A., GO Universidade Federal de Jataí UFJ, GO Universidade Estadual Paulista UNESP, SP Escola Superior de Agricultura “Luiz de Queiroz” ESALQ/USP, SP Universidade Estadual Paulista UNESP, SP
Published: 2021
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