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Species-specific differentiation of Bordetella pathogens in nasopharyngeal swabs of patients with suspected whooping cough was carried out using developed by us real-time PCR: duplex TaqMan PCR aimed for detecting B. pertussis and B. parapertussis, with primers targeting IS481, IS1001, BP0026 and GAPDH as the human internal control and two singleplex TaqMan PCR aimed for detecting B. holmesii and B. bronchiseptica with primers targeting hIS1001 and flaA specific genes. Among the 3130 throat swab analyzed, B. pertussis, B. parapertussis and B. holmesii were detected in 29.5 %, 0.9 % and 0.4 % of samples respectively. The developed algorithm proved to be a useful molecular-genetic diagnostic tool for whooping cough, established cocirculation of different species of Bordetella genus and demonstrated the predominance of B. pertussis, low rate of B. parapertussis and B. holmesii and the absence of B. bronchiseptica in the circulation in the Republic of Belarus.

The growth characteristics of a 30-year-old Pinus sibirica grafted with cuttings from plus trees and a 36-year-old one of different geographical origin on the rootstock of Pinus sylvestris and Pinus sibirica, the results of their studying are presented in the article. The analysis of accretion of grafted components depending on the type, age of the rootstock was done. It has been established that 36-year-old grafted trees using cuttings of Pinus Sibirica of different geographical origin (Altai, Biryusa, Sverdlovsk, Khanty-Mansi) on the Pinus sylvestris tree differ in the growth of scion with rootstock. Ramets with good accretion (without exceeding the diameter of the stem of the scion over the stock) with maximum incompatibility (13.1 cm) were selected. The growth of 30-year-old grafted trees depends on the genotype of plus trees. The most intensive growth of ramet is noted at clone 91/55 in comparison with 100/64 and 94/58. The grafted trees of one clone (91/55) on the Pinus sylvestris had an average graft stem excess of 6.2 cm; there is practically no excess on the Pinus sibirica stock. It is recommended to use the selected specimens without sagging in the places of accretion of grafted components as uterine trees for their further reproduction and the creation of target plantations for the accelerated cultivation and use of industrial wood from Pinus sibirica.