1. Development of a new RT-PCR with multiple primers for detecting Southern African Territories foot-and-mouth disease viruses
- Author
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Qiang Zhang, Huiyun Chang, Yongguang Zhang, Wang Jun, Jie Zhang, Ya-Li Liu, Xiao-yuan Ma, He Jijun, Yong-sheng Liu, Ma Bing, Jianliang Lv, Dai Junfei, Yonglu Wang, Xiangtao Liu, Wei-Min Ma, Ou Yunwen, and Ding Yaozhong
- Subjects
0301 basic medicine ,Serotype ,diagnosis ,animal diseases ,viruses ,rt-pcr ,Veterinary medicine ,specificity ,Biology ,Genome ,Virus ,southern african territory foot-and-mouth disease ,03 medical and health sciences ,SF600-1100 ,medicine ,Multiplex ,Gene ,General Veterinary ,Foot-and-mouth disease ,Amplicon ,medicine.disease ,sensitivity ,Virology ,Swine Vesicular Disease Virus ,030104 developmental biology ,Research Article - Abstract
Introduction: The extremely high genetic variation and the continuously emerging variants of foot-and-mouth disease virus (FMDV) of Southern African Territory (SAT) serotypes including SAT1, SAT2, and SAT3 make it necessary to develop a new RT-PCR for general use for monitoring viruses based on the updated genome information. Material and Methods: A FMDV SAT-D8 one-step RT-PCR was established based on the 1D2A2B genes of the SAT serotype viruses with a multiplex primer set. FMDV A, O, C, and Asia 1 serotypes, other vesicular disease viruses, inactivated SAT viruses, and 125 bovine, ovine, caprine and porcine tissue samples collected from the Chinese mainland were included for evaluating the assay. Results: The new RT-PCR was proven to be specific without cross-reactions with Eurasian FMDV, swine vesicular disease virus (SVDV), Seneca valley virus (SVV), or other common viral pathogens of cattle, sheep, goat, and pig. An around 257 bp-sized amplicon clearly appeared when the inactivated SAT viruses were detected. However, all 125 samples collected from FMDV-susceptible animals from the Chinese mainland which has not known SAT epidemics showed negative results. Conclusions: A FMDV SAT-D8 one-step RT-PCR is a promising method for primary screening for FMDV SAT serotypes.
- Published
- 2018