313 results on '"Yogo, A."'
Search Results
2. SNAIL2 contributes to tumorigenicity and chemotherapy resistance in pancreatic cancer by regulating IGFBP2
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Toshihiko Masui, Shigeo Takaishi, Kenji Masuo, Ru Chen, Aiko Sugiyama, Akihisa Fukuda, Hiroshi Seno, Akitada Yogo, and Shinji Uemoto
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cancer stem cells ,Cancer Research ,Epithelial-Mesenchymal Transition ,Carcinogenesis ,pancreatic cancer ,Antineoplastic Agents ,Mice, SCID ,Cell Line ,SNAIL2 ,Cell, Molecular, and Stem Cell Biology ,Cancer stem cell ,RNA interference ,Mice, Inbred NOD ,Pancreatic cancer ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Epithelial–mesenchymal transition ,Transcription factor ,epithelial‐mesenchymal transition ,biology ,Microarray analysis techniques ,business.industry ,Gene Expression Profiling ,CD44 ,General Medicine ,Original Articles ,medicine.disease ,tumor spheroid ,Xenograft Model Antitumor Assays ,Gemcitabine ,Gene Expression Regulation, Neoplastic ,Pancreatic Neoplasms ,Insulin-Like Growth Factor Binding Protein 2 ,HEK293 Cells ,Oncology ,Drug Resistance, Neoplasm ,biology.protein ,Cancer research ,Neoplastic Stem Cells ,Original Article ,RNA Interference ,Snail Family Transcription Factors ,business ,medicine.drug - Abstract
Pancreatic cancer has an extremely poor prognosis because of its resistance to conventional therapies. Cancer stem cell (CSC)‐targeted therapy is considered a promising approach for this disease. Epithelial‐mesenchymal transition‐inducing transcription factors (EMT‐TFs) contribute to CSC properties in some solid tumors; however, this mechanism has not been fully elucidated in pancreatic cancer. Zinc finger protein, SNAIL2 (also known as SLUG), is a member of the SNAIL superfamily of EMT‐TFs and is commonly overexpressed in pancreatic cancer. Patients exhibiting high SNAIL2 expression have a poor prognosis. In this study, we showed that the suppression of SNAIL2 expression using RNA interference decreased tumorigenicity in vitro (sphere formation assay) and in vivo (xenograft assay) in 2 pancreatic cancer cell lines, KLM1 and KMP5. In addition, SNAIL2 suppression resulted in increased sensitivity to gemcitabine and reduced the expression of CD44, a pancreatic CSC marker. Moreover, experiments on tumor spheroids established from surgically resected pancreatic cancer tissues yielded similar results. A microarray analysis revealed that the mechanism was mediated by insulin‐like growth factor (IGF) binding protein 2. These results indicate that IGFBP2 regulated by SNAIL2 may represent an effective therapeutic target for pancreatic cancer., SNAIL2 knockdown reduces tumorigenicity and resistance to gemcitabine in tumor spheroid established from resected tissue of human pancreatic cancer as well as human pancreatic cancer cell lines.
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- 2021
3. NMR assignments of the N-glycans of the Fc fragment of mouse immunoglobulin G2b glycoprotein
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Yohei Miyanoiri, Yoshiki Yamaguchi, Ichio Shimada, Takeshi Takizawa, Koichi Kato, Rina Yogo, and Saeko Yanaka
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chemistry.chemical_classification ,0303 health sciences ,Glycan ,biology ,Molecular mass ,Effector ,Stereochemistry ,030303 biophysics ,Nuclear magnetic resonance spectroscopy ,Biochemistry ,Fragment crystallizable region ,Immunoglobulin G ,03 medical and health sciences ,chemistry ,Structural Biology ,biology.protein ,Antibody ,Glycoprotein ,030304 developmental biology - Abstract
The Fc portion of immunoglobulin G (IgG) promotes defensive effector functions in the immune system by interacting with Fcγ receptors and complement component C1q. These interactions critically depend on N-glycosylation at Asn297 of each CH2 domain, where biantennary complex-type oligosaccharides contain microheterogeneities resulting primarily from the presence or absence of non-reducing terminal galactose residues. Crystal structures of Fc have shown that a pair of N-glycans is located between the two CH2 domains. Here we applied our metabolic isotope labeling technique using mammalian cells for in-solution structural characterization of mouse IgG2b-Fc glycoforms with a molecular mass of 54 kDa. Based on spectral assignments of the N-glycans as well as polypeptide backbones of Fc, we probed conformational perturbations of Fc induced by N-glycan trimming, especially enzymatic degalactosylation. The results indicated that degalactosylation structurally perturbed the Fc region through rearrangement of glycan-protein interactions. The spectral assignments of IgG2b-Fc glycoprotein will provide the basis for NMR investigation of its dynamic conformations and interactions with effector molecules in solution.
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- 2021
4. Impact of vascular abnormality on contrast-enhanced CT and high C-reactive protein levels on postoperative pancreatic hemorrhage after pancreaticoduodenectomy: A multi-institutional, retrospective analysis of 590 consecutive cases
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Kenzo Nakano, Toshihiko Masui, Asahi Sato, Yuichiro Uchida, Shinji Uemoto, Kyoichi Takaori, Kazuyuki Nagai, Takayuki Anazawa, Takafumi Machimoto, Kazuki Hashida, and Akitada Yogo
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Adult ,Male ,medicine.medical_specialty ,Endocrinology, Diabetes and Metabolism ,medicine.medical_treatment ,Postoperative Hemorrhage ,Gastroenterology ,Pancreaticoduodenectomy ,Pancreatic Fistula ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Predictive Value of Tests ,Risk Factors ,Internal medicine ,Ascites ,medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,Hepatology ,biology ,business.industry ,C-reactive protein ,Perioperative ,Odds ratio ,Middle Aged ,medicine.disease ,C-Reactive Protein ,Pancreatic fistula ,030220 oncology & carcinogenesis ,biology.protein ,Blood Vessels ,Female ,030211 gastroenterology & hepatology ,Abnormality ,medicine.symptom ,Tomography, X-Ray Computed ,business ,Complication ,Aneurysm, False - Abstract
Background /Objectives: This study aimed to elucidate the efficacy of CT findings and perioperative characteristics to predict post-pancreatectomy hemorrhage (PPH): a critical complication after pancreaticoduodenectomy. Methods The records of 590 consecutive patients who underwent pancreaticoduodenectomy at three institutes between 2012 and 2018 were included. The presence of a vascular wall abnormality or ascites with high density (vascular abnormality) on postoperative day (POD) 5–10 contrast-enhanced CT (early CT), perioperative characteristics, and any PPH or pseudoaneurysm formation (PPH events) were analyzed through a multivariate analysis. Results PPH events occurred in 48 out of 590 patients (8%). The vascular abnormality on early CT and the C-reactive protein (CRP) value on POD 3 were independent risk factors for PPH events after POD5 (vascular abnormality: odds ratio 6.42, p = 0.001; CRP on POD 3: odds ratio 1.17, p = 0.016). The sensitivity of vascular abnormality for PPH events was 24% (7/29), and the positive predictive value was 30% (7/23). The combination of vascular abnormality and a high CRP value (≥15.5 mg/dL) on postoperative day 3 had a higher positive predictive value of 64% (7/11) than the vascular abnormality alone. None of the seven PPH events that occurred more than one month after surgery were foreseen via early CT. Conclusion The combination of vascular abnormality and high CRP value was associated with increasing risk of PPH events after pancreaticoduodenectomy, but the low sensitivity of early CT must be noted as an important shortcoming. The normal findings on early CT could not eliminate the risk of late PPH.
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- 2021
5. Development of Corcyra cephalonica (Stainton) on six varieties of brown and milled rice
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Fatchuliani Safitri Ramadhani, Helen Angelina Sitanggang, Yogo Setiawan, Ludji Pantja Astuti, Akhmad Rizali, and Mutala’liah
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Horticulture ,Corcyra cephalonica ,Insect Science ,Biology - Published
- 2021
6. Dlec1 is required for spermatogenesis and male fertility in mice
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Takahiro Yamagata, Hideo Dohra, Wataru Fujii, Keiichiro Yogo, Mamoru Nagano, Kiyotaka Toshimori, Yu Okitsu, and Chizuru Ito
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Male ,Reproductive biology ,lcsh:Medicine ,Biology ,Ion Channels ,Article ,Male infertility ,Mice ,Tubulin ,Intraflagellar transport ,medicine ,Animals ,Humans ,Spermatogenesis ,lcsh:Science ,Infertility, Male ,Multidisciplinary ,Tumor Suppressor Proteins ,Cilium ,HEK 293 cells ,lcsh:R ,medicine.disease ,Spermatozoa ,Phenotype ,Sperm ,Cell biology ,HEK293 Cells ,A549 Cells ,Organ Specificity ,Differentiation ,Adenocarcinoma ,lcsh:Q ,CRISPR-Cas Systems ,Gene Deletion - Abstract
Deleted in lung and esophageal cancer 1 (DLEC1) is a tumour suppressor gene that is downregulated in various cancers in humans; however, the physiological and molecular functions of DLEC1 are still unclear. This study investigated the critical role of Dlec1 in spermatogenesis and male fertility in mice. Dlec1 was significantly expressed in testes, with dominant expression in germ cells. We disrupted Dlec1 in mice and analysed its function in spermatogenesis and male fertility. Dlec1 deletion caused male infertility due to impaired spermatogenesis. Spermatogenesis progressed normally to step 8 spermatids in Dlec1−/− mice, but in elongating spermatids, we observed head deformation, a shortened tail, and abnormal manchette organization. These phenotypes were similar to those of various intraflagellar transport (IFT)-associated gene-deficient sperm. In addition, DLEC1 interacted with tailless complex polypeptide 1 ring complex (TRiC) and Bardet–Biedl Syndrome (BBS) protein complex subunits, as well as α- and β-tubulin. DLEC1 expression also enhanced primary cilia formation and cilia length in A549 lung adenocarcinoma cells. These findings suggest that DLEC1 is a possible regulator of IFT and plays an essential role in sperm head and tail formation in mice.
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- 2020
7. Tyrosinase Inhibition, Antiglycation, and Antioxidant Activity of Xylocarpus granatum
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Irmanida Batubara, Wulan Tri Wahyuni, Waras Nurcholis, Erna Subroto, Yogo Suro Priyadi, Kilala Tilaar, Saat Egra, Maily Mustofa, Fransiska Devy Junardy, and Nevianti Zamany
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Antioxidant ,DPPH ,medicine.medical_treatment ,Tyrosinase ,Fractionation ,antiglycation, antioxidant, tyrosinase inhibition, xylocarpus granatum ,030226 pharmacology & pharmacy ,lcsh:Education (General) ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Xylocarpus granatum ,medicine ,Potency ,Bovine serum albumin ,lcsh:QH301-705.5 ,General Environmental Science ,biology ,Traditional medicine ,Chemistry ,General Engineering ,Fructose ,biology.organism_classification ,lcsh:Biology (General) ,030220 oncology & carcinogenesis ,biology.protein ,General Earth and Planetary Sciences ,lcsh:L7-991 - Abstract
Xylocarpus granatum is mangrove plant that traditionally used as face powder in Central Sulawesi, Indonesia which related to antioxidant, antiglycation and tyrosinase inhibition activities. This study aimed to evaluate the potency of X. granatum as a tyrosinase inhibitor, antiglycation, and antioxidant. The leaves, stem, stem bark, fruit flesh, fruit peel, and kernel of X. granatum were extracted using ethanol then their tyrosinase inhibition, antiglycation, and antioxidant were evaluated. Tyrosinase inhibition activity was evaluated using in vitro assay with L-tyrosine and L-DOPA as the substrate of monophenolase and diphenolase. Antiglycation activity was studied by measuring the excitation and emission fluorescence from glucose and fructose reaction with Bovine Serum Albumin. Antioxidant activity was measured using the DPPH radical scavenging assay. The result showed that the ethanolic extract of fruit flesh has higher potency as tyrosinase inhibitor (IC 50 of 393.8 mg/L and IC 50 of 448 mg/L, respectively for monophenolase and diphenolase). Antiglycation assay showed that the ethanolic extract of stem bark provides the strongest antiglycation activity with an IC 50 of 118.1 mg/L. Meanwhile, fruit peel provides the strongest antioxidant activity with an IC 50 of 5.5 mg/L. Fractionation of ethanolic extracts of each part of X. granatum tree yield fractions with lower bioactivity compared to the crude extract. Moreover, stem extract and fractions from two different locations (Tarakan and Kendari) tend to have different bioactivities strengths. The stem part of X granatum could be developed as new raw material of cosmetic product in Indonesia, while ethanol as the solvent for extraction, and the different bioactivity of stem extract from different location can be the consideration for the industry to standardize the extract prior to production of final product.
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- 2020
8. Population growth and development of Carpophilus hemipterus (L.) on cashew nut (Anacardium occidentale L.) in the storage
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Yogo Setiawan, Ludji Pantja Astuti, Putri Dewi Sartika, and Mutala’liah
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Toxicology ,Pupa ,Larva ,Carpophilus hemipterus ,Insect Science ,Anacardium ,fungi ,Population growth ,Diet weight loss ,Biology ,Cashew nut ,biology.organism_classification ,Population density - Abstract
This research was conducted to obtain the information about the population growth and development of C. hemipterus on cashew nut by considering the initial population density and feed availability, thus it can be expected to eliminate the damage on cashew nut in the storage. Study was carried out under laboratory condition by no-choice test for each treatment. There were two factors as a treatment combination, i.e. initial population density (1, 5, 10, 20, and 40 pairs) and diet quantity (25, 50, and 100 g). The variables observed on this research were mortality of infested adults, population growth (number of eggs, larvae, pupae, and F1 progeny), development period from egg to adult, and diet weight loss. Results showed that the number of eggs, larvae, pupae, and F1 progeny emerged were higher on 40 pairs of adult insects with 25 g diet and the development period from egg to adult was last for 41.26 days. Diet weight loss was influenced by initial population density and diet quantity with the higher loss was on 40 pairs of adult insects and 50 g diet.
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- 2020
9. Short Communication: Nematodes associated with Robusta coffee plantations in Malang District, East Java, Indonesia
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Yogo Setiawan, Eyik Widyansyaficha Marsudi, Titi Widjayanti, and Hagus Tarno
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Plant Science ,Biology ,biology.organism_classification ,Diversity index ,Nematode ,Agronomy ,Abundance (ecology) ,Genus ,Soil pH ,Xiphinema ,Animal Science and Zoology ,Helicotylenchus ,Pratylenchus ,Molecular Biology - Abstract
Tarno H, Marsudi EW, Widjayanti T, Setiawan Y. 2021. Short Communication: Nematodes associated with Robusta coffee plantations in Malang District, East Java, Indonesia. Biodiversitas 22: 3306-3312. Coffee (Coffea spp.) is an important commodity in Indonesia. Nematodes have different roles, such as plant parasites and non-parasites, and are commonly found in the soil of coffee plantations. This research determined the ecological role, diversity, and abundance of nematodes in three coffee plantations in Malang District, East Java. We used a Whitehead tray to extract and isolate nematodes from the soil and root samples. Nematodes were identified based on their morphological characteristics. Analysis of variance and diversity indices were used to identify the differences among three coffee plantations. We identified eight nematode genera in the Ngantang sub-district site. In the Jatikerto Agrotechnopark and University of Brawijaya Forest sites, six genera were identified. Criconemoides, Pratylenchus, Xiphinema, Helicotylenchus are plant-parasitic nematodes, and Mononchus, Dorylaimus, Rhabditis, and Aphanolaimus are non-plant parasitic nematodes. Non-plant parasitic nematodes were more abundant than plant-parasitic nematodes in all sites. Non-plant parasitic nematode, Dorylaimus was the dominant genus in this study (272 individuals), ca. 35% of the total nematodes collected. In this study, the differences between coffee plantations and soil pH influence nematode abundance. The nematode abundance increases when the soil pH is lower.
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- 2021
10. Short Communication: First record of the cassava lace bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) from East Java, Indonesia
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Ito Fernando, Dewi Anggraini, Hammam Abdullah Rizqi, Yogo Setiawan, and Retno Dyah Puspitarini
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biology ,Java ,QH301-705.5 ,southeast asia ,Heteroptera ,Manihot esculenta ,manihot esculenta ,Euphorbiaceae ,Plant Science ,invasion ,biology.organism_classification ,Hemiptera ,Tingidae ,Southeast asia ,Geography ,neotropics ,Botany ,Animal Science and Zoology ,Biology (General) ,exotic pest ,Alien species ,Molecular Biology ,computer ,computer.programming_language - Abstract
Puspitarini RD, Fernando I, Setiawan Y, Anggraini D, Rizqi HA. 2021. Short Communication: First record of the cassava lace bug Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) from East Java, Indonesia. Biodiversitas 22: 2870-2876. The cassava lace bug, Vatiga illudens (Drake, 1922) (Hemiptera: Heteroptera: Tingidae) is recorded from Indonesia for the first time. This species is found on the abaxial surface of leaves of cassava (Manihot esculenta Crantz, Euphorbiaceae) in several plantations of East Java and heavily infest this plant. Vatiga illudens is native to the Neotropical Region and seems to be the alien species in the Oriental Region. This tingid has already spread over a wide region of East Java, suggesting that the invasion of the lace bug endangers the other regions cultivating cassava.
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- 2021
11. The development of unlabeled probes-high resolution melting (UP-HRM) marker on SAD, IAA27 and ACC genes of oil palm
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Olivia Sriulina Purba, Tony Liwang, Wulan Artutiningsih, Yogo Adhi Nugroho, Condro Utomo, Roberdi Roberdi, Dwi Yono, Sigit Dwi Maryanto, and Tengku Imam Saputra
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0106 biological sciences ,Genetics ,0303 health sciences ,biology ,QH301-705.5 ,Dendrogram ,Single-nucleotide polymorphism ,Heterozygote advantage ,Plant Science ,biology.organism_classification ,Elaeis guineensis ,01 natural sciences ,High Resolution Melt ,03 medical and health sciences ,Elaeis oleifera ,hrm, interspecific hybrid, molecular markers, oil palm, snps genotyping ,Genotype ,SNP ,Animal Science and Zoology ,Biology (General) ,Molecular Biology ,030304 developmental biology ,010606 plant biology & botany - Abstract
Saputra TI, Roberdi, Nugroho YA, Artutiningsih W, Purba OS, Maryanto SD, Yono D, Utomo C, Liwang T. 2021. The development of unlabeled probes-high resolution melting (UP-HRM) marker on SAD, IAA27 and ACC genes of oil palm. Biodiversitas 22: 3356-3362. The unlabeled probes-high resolution melting (UP-HRM) marker is a useful tool for detecting of single nucleotide polymorphisms (SNPs). The objectives of this study were to develop UP-HRM markers to differentiate specific SNPs patterns on oil palm. The marker was developed and tested with Elaeis guineensis (Eg), Elaeis oleifera (Eo), Eo x Eg (hybrid), and was validated with 53 individuals of BC1F1 populations ((Eo x Eg) x Eg). Four UP-HRM markers were developed based on 2 SNPs in the stearoyl-acyl-carrier-protein 9-desaturase (EgSAD), 1 SNP in the auxin-responsive protein IAA27-like (EgIAA27), and 1 SNP in the 1-amino cyclopropane-1-carboxylate oxidase (EgACC) genes. The SNP discovery result showed that Eg was represented a reference homozygote genotype, while Eo was represented as an alternative homozygote genotype and the Eo x Eg hybrid was represented as a heterozygote genotype in all genes. The typical UP-HRM melt curve graph was successfully generated. This result was consistent with each genotype model for all four markers. The UP-HRM markers can distinguish each genotype according to the single-pass sequencing results. Furthermore, dendrogram analysis on validation divided 53 BC1F1 samples into three cluster groups.
- Published
- 2021
12. Genomewide SNP marker identification associated with drought tolerance in oil palm
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Zulfikar Achmad Tanjung, Tony Liwang, Yogo Adhi Nugroho, Dwi Yono, and Condro Utomo
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0106 biological sciences ,0301 basic medicine ,Abiotic component ,QH301-705.5 ,Drought tolerance ,Bulked segregant analysis ,food and beverages ,Single-nucleotide polymorphism ,Plant Science ,Biology ,01 natural sciences ,03 medical and health sciences ,Horticulture ,030104 developmental biology ,Palm oil ,SNP ,Animal Science and Zoology ,bulked segregant analysis, ddrad, drought stress, ffb production, snp ,Biology (General) ,Palm ,Molecular Biology ,Genotyping ,010606 plant biology & botany - Abstract
Yono D, Nugroho YA, Tanjung ZA, Utomo C, Liwang T. 2021. Genomewide SNP marker identification associated with drought tolerance in oil palm. Biodiversitas 22: 3138-3144. Drought stress is one of the abiotic stresses that frequently occurred in the oil palm plantation and has a negative impact on fresh fruit bunch (FFB) production. Therefore, drought-tolerant palms are essential to be selected to mitigate this challenge. In Indonesia, several oil palm plantation areas have a dry climate, such as Lampung province. Distinct yield performance palms were identified from well-recorded agronomic trials in these areas, where the palms are frequently exposed to drought stresses every year and lead them to suffer from water deficit response. Group of high and low-yielding palms was selected based on FFB production of each palm for at least ten constitutive years. The double digest restriction amplified DNA (ddRAD) genotyping methods were used to capture the Single Nucleotide Polymorph (SNP) variant from pools of sample association datasets. At least, 538k SNPs were identified from these pooled datasets. A bulked segregant analysis with a Case-Control approach was implemented to screen the contrast SNP profiles between both pools. A total of 56 association signals was selected from sequential filtering. These SNP sites are located in 21 genes. Further SNP validation and phenotypic verification are necessary to obtain SNPs marker for drought-tolerant palm selections.
- Published
- 2021
13. Small-Scale Panel Comprising Diverse Gene Family Targets To Evaluate Compound Promiscuity
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Taisuke Katoh, Tomoya Sameshima, Masato Yoshikawa, Tomoya Yukawa, Hideto Hara, Takatoshi Yogo, Russell Naven, Makoto Miyamoto, Ikuo Miyahisa, Yoshihiko Hirozane, and Teruaki Okuda
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Drug ,Drug-Related Side Effects and Adverse Reactions ,Cell Survival ,media_common.quotation_subject ,Drug Evaluation, Preclinical ,010501 environmental sciences ,Biology ,Toxicology ,Bioinformatics ,01 natural sciences ,Mice ,03 medical and health sciences ,Receptors, Glucocorticoid ,Receptor, Serotonin, 5-HT2B ,Animals ,Humans ,Gene family ,Adverse effect ,030304 developmental biology ,0105 earth and related environmental sciences ,media_common ,rho-Associated Kinases ,0303 health sciences ,Drug discovery ,Receptor, Adenosine A3 ,Receptor, Muscarinic M1 ,Biological activity ,Hep G2 Cells ,General Medicine ,Receptors, GABA-A ,Cyclic Nucleotide Phosphodiesterases, Type 4 ,Rats ,PPAR gamma ,Promiscuity ,Drug development ,Toxicity - Abstract
Despite the recent advances in the life sciences and the remarkable investment in drug discovery research, the success rate of small-molecule drug development remains low. Safety is the second most influential factor of drug attrition in clinical studies; thus, the selection of compounds with fewer toxicity concerns is crucial to increase the success rate of drug discovery. Compounds that promiscuously bind to multiple targets are likely to cause unexpected pharmacological activity that may lead to adverse effects. Therefore, avoiding such compounds during early research stages would contribute to identifying compounds with a higher chance of success in the clinic. To evaluate the interaction profile against a wide variety of targets, we constructed a small-scale promiscuity panel (PP) consisting of eight targets (ROCK1, PDE4D2, GR, PPARγ, 5-HT2B, adenosine A3, M1, and GABAA) that were selected from diverse gene families. The validity of this panel was confirmed by comparison with the promiscuity index evaluated from larger-scale panels. Analysis of data from the PP revealed that both lipophilicity and basicity are likely to increase promiscuity, while the molecular weight does not significantly contribute. Additionally, the promiscuity assessed using our PP correlated with the occurrence of both in vitro cytotoxicity and in vivo toxicity, suggesting that the PP is useful to identify compounds with fewer toxicity concerns. In summary, this small-scale and cost-effective PP can contribute to the identification of safer compounds that would lead to a reduction in drug attrition due to safety issues.
- Published
- 2019
14. The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae
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Hagus Tarno, Abdul Latief Abadi, Yogo Setiawan, Masruri Masruri, Mochammad Syamsul Hadi, Luqman Qurata Aini, Safira Rizka Lestari, Toto Himawan, and Bambang Tri Rahardjo
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QH301-705.5 ,Plant Science ,Serratia ,Toxicology ,03 medical and health sciences ,chemistry.chemical_compound ,Biology (General) ,Molecular Biology ,030304 developmental biology ,biodegradation, chlorpyrifos, digestive insect, plutella xylostella, symbiont bacteria ,0303 health sciences ,biology ,030306 microbiology ,business.industry ,fungi ,Pseudomonas ,Pest control ,food and beverages ,Plutella ,Pesticide ,biology.organism_classification ,chemistry ,Chlorpyrifos ,Animal Science and Zoology ,PEST analysis ,business ,Bacteria - Abstract
Hadi MS, Abadi AL, Himawan T, MAsruri, Lestari SR, Rahardjo BT, Aini LQ, Setiawan Y, Tarno H. 2021. The role of bacterial symbionts in the biodegradation of chlorpyrifos in the digestive tract of Plutella xylostella larvae. Biodiversitas 22: 702-712. Several species in the order Lepidoptera act as plant pests, one of which is Plutella xylostella. Plutella xylostella is one of the most destructive pests of cabbage and other horticultural crops. The use of chemical insecticides as pest control for P. xylostella causes many problems, such as the increased pest resistance to pesticides. The objectives of this study are: (i) to obtain and characterize symbiont bacteria in the digestive tract of P. xylostella collected from organic and conventional agriculture soils; (ii) to evaluate the potential of bacterial symbionts in the digestive tract of P. xylostella from organic and conventional soils in degrading the active ingredient of chlorpyrifos insecticide; (iii) To determine the biodegradation process of chlorpyrifos insecticide by symbiont bacteria in the digestive tract of P. xylostella; and (iv) to identify the derivative compounds from the biodegradation of chlorpyrifos insecticide. The results showed 30 symbiont bacteria isolated from the digestive tract of P. xylostella collected from organic soil and 36 symbiont bacteria isolated from the digestive tract of P. xylostella from conventional farming soil. There are 15 species of symbiont bacteria in 5 genera from the digestive tract of P. xylostella from organic and conventional farming capable of degrading the chlorpyrifos insecticide. They are identified as Providencia sp., Pseudomonas sp., Serratia sp., Proteus sp., and Aeromonas sp. Chlorpyrifos-derived compounds from the biodegradation of symbiont bacteria are less toxic than chlorpyrifos compounds.
- Published
- 2021
15. Time–frequency analysis of serum with proton nuclear magnetic resonance for diagnosis of pancreatic cancer
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Keiko Hirakawa, Asahi Sato, Kaoru Koike, Toshihiko Masui, Shinji Uemoto, Akitada Yogo, Takashi Ito, and Yoshimasa Kanawaku
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0301 basic medicine ,Adult ,Male ,medicine.medical_specialty ,CA-19-9 Antigen ,Science ,medicine.medical_treatment ,Liver transplantation ,Gastroenterology ,Article ,03 medical and health sciences ,0302 clinical medicine ,Text mining ,Carcinoembryonic antigen ,Internal medicine ,Pancreatic cancer ,Medicine ,Humans ,Nuclear Magnetic Resonance, Biomolecular ,Cancer ,Aged ,Aged, 80 and over ,Multidisciplinary ,biology ,business.industry ,Diagnostic markers ,Middle Aged ,Serum samples ,medicine.disease ,Carcinoembryonic Antigen ,Pancreatic Neoplasms ,030104 developmental biology ,030220 oncology & carcinogenesis ,Proton NMR ,biology.protein ,Female ,business ,Carbohydrate antigen ,Biomarkers ,Serum markers - Abstract
Although serum markers such as carcinoembryonic antigen (CEA) and carbohydrate antigen (CA19-9) have been widely used in screening for pancreatic cancer (PC), their sensitivity and specificity are unsatisfactory. Recently, a novel tool of analyzing serum using the short-time Fourier transform (STFT) of free induction decays (FIDs) obtained by 1H-NMR has been introduced. We for the first time evaluated the utility of this technology as a diagnostic tool for PC. Serum was obtained from PC patients before starting any treatments. Samples taken from individuals with benign diseases or donors for liver transplantation were obtained as controls. Serum samples from both groups underwent 1H-NMR and STFT of FIDs. STFT data were analyzed by partial least squares discriminant analysis (PLS-DA) to clarify whether differences were apparent between groups. As a result, PLS-DA score plots indicated that STFT of FIDs enabled effective classification of groups with and without PC. Additionally, in a subgroup of PC, long-term survivors (≥ 2 years) could be discriminated from short-term survivors (1H-NMR have a potential to be a diagnostic and prognostic tool of PC.
- Published
- 2020
16. Development of an immuno-wall device for the rapid and sensitive detection of EGFR mutations in tumor tissues resected from lung cancer patients
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Tetsunari Hase, Yoshinori Hasegawa, Yoshinobu Baba, Takahiro Hatta, Manabu Tokeshi, Kohei Matsuoka, Mitsuo Sato, Toshihiro Kasama, Toyofumi F. Chen-Yoshikawa, Noritada Kaji, Kazuki Komeda, Nozomi Kawabe, Keine Nishiyama, Hirofumi Shibata, Naoyuki Yogo, and Naoya Ozawa
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0301 basic medicine ,Lung Neoplasms ,medicine.medical_treatment ,DNA Mutational Analysis ,Gene Identification and Analysis ,Lung and Intrathoracic Tumors ,Targeted therapy ,Exon ,0302 clinical medicine ,Genotype ,Medicine and Health Sciences ,Medicine ,Epidermal growth factor receptor ,Enzyme-Linked Immunoassays ,Fluorescence Immunoassay ,Immunoassay ,Multidisciplinary ,biology ,medicine.diagnostic_test ,ErbB Receptors ,Deletion Mutation ,Oncology ,030220 oncology & carcinogenesis ,Calibration ,Antibody ,Research Article ,Substitution Mutation ,Imaging Techniques ,Science ,Research and Analysis Methods ,Fluorescence ,03 medical and health sciences ,Cell Line, Tumor ,Fluorescence Imaging ,Genetics ,Humans ,Lung cancer ,Immunoassays ,Genotyping ,Immunohistochemistry Techniques ,Mutation Detection ,business.industry ,Cancers and Neoplasms ,Biology and Life Sciences ,medicine.disease ,respiratory tract diseases ,Non-Small Cell Lung Cancer ,Histochemistry and Cytochemistry Techniques ,030104 developmental biology ,Mutation ,Cancer research ,biology.protein ,Immunologic Techniques ,business - Abstract
Detecting molecular targets in specimens from patients with lung cancer is essential for targeted therapy. Recently, we developed a highly sensitive, rapid-detection device (an immuno-wall device) that utilizes photoreactive polyvinyl alcohol immobilized with antibodies against a target protein via a streptavidin–biotin interaction. To evaluate its performance, we assayed epidermal growth factor receptor (EGFR) mutations, such as E746_A750 deletion in exon 19 or L858R substitution in exon 21, both of which are common in non-small cell lung cancer and important predictors of the treatment efficacy of EGFR tyrosine kinase inhibitors. The results showed that in 20-min assays, the devices detected as few as 1% (E746_A750 deletion) and 0.1% (L858R substitution) of mutant cells. Subsequent evaluation of detection of the mutations in surgically resected lung cancer specimens from patients with or without EGFR mutations and previously diagnosed using commercially available, clinically approved genotyping assays revealed diagnostic sensitivities of the immuno-wall device for E746_A750 deletion and L858R substitution of 85.7% and 87.5%, respectively, with specificities of 100% for both mutations. These results suggest that the immuno-wall device represents a good candidate next-generation diagnostic tool, especially for screening of EGFR mutations.
- Published
- 2020
17. Analysis of codon usage bias reveals optimal codons in Elaeis guineensis
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Redi Aditama, Yogo Adhi Nugroho, Zulfikar Achmad Tanjung, Condro Utomo, Widyartini Made Sudania, and Tony Liwang
- Subjects
0106 biological sciences ,Genetics ,Codon Adaptation Index ,0303 health sciences ,biology ,QH301-705.5 ,Plant Science ,elaeis guineensis, gc content, synonymous codons ,Elaeis guineensis ,biology.organism_classification ,Positive correlation ,010603 evolutionary biology ,01 natural sciences ,Genome ,Unimodal distribution ,03 medical and health sciences ,Codon usage bias ,Animal Science and Zoology ,Biology (General) ,Molecular Biology ,Gene ,GC-content ,030304 developmental biology - Abstract
Aditama R, Tanjung ZA, Sudania WM, Nugroho YA, Utomo C, Liwang T. 2020. Analysis of codon usage bias reveals optimal codons in Elaeis guineensis. Biodiversitas 21: 5331-5337. Codon usage bias of oil palm genome was reported employing several indices, including GC content, relative synonymous codon usage (RSCU), the effective number of codons (ENC), and codon adaptation index (CAI). Unimodal distribution of GC content was observed and matched with non-grass monocots characteristics. Correspondence analysis (COA) on synonymous codon usage bias showed that the main axis was strongly driven by GC content. The ENC and neutrality plot of oil palm genes indicating that natural selection played more vital role compared to mutational bias on shaping codon usage bias. A positive correlation between calculated CAI and experimental data of oil palm gene expression was detected indicating good ability of this index. Finally, eighteen codons were defined as “optimal codons” that may provide a useful reference for heterogeneous expression and genome editing studies.
- Published
- 2020
18. Silkworm Pupae Function as Efficient Producers of Recombinant Glycoproteins with Stable-Isotope Labeling
- Author
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Rina Yogo, Jun Yokoyama, Koichi Kato, Saeko Yanaka, Enoch Y. Park, Hirokazu Yagi, Masayoshi Onitsuka, Tatsuya Kato, Toshio Yamazaki, and Akari Ikeda
- Subjects
0301 basic medicine ,animal structures ,lcsh:QR1-502 ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Immunoglobulin G ,Article ,lcsh:Microbiology ,law.invention ,silkworm pupa ,03 medical and health sciences ,chemistry.chemical_compound ,immunoglobulin G ,recombinant glycoprotein ,law ,Animals ,isotope labeling ,Molecular Biology ,Glycoproteins ,chemistry.chemical_classification ,Methionine ,biology ,fungi ,Pupa ,Bombyx ,artificial diet ,Yeast ,Recombinant Proteins ,0104 chemical sciences ,030104 developmental biology ,chemistry ,biology.protein ,Recombinant DNA ,Composition (visual arts) ,Antibody ,Glycoprotein ,Methyl group - Abstract
Baculovirus-infected silkworms are promising bioreactors for producing recombinant glycoproteins, including antibodies. Previously, we developed a method for isotope labeling of glycoproteins for nuclear magnetic resonance (NMR) studies using silkworm larvae reared on an artificial diet containing 15N-labeled yeast crude protein extract. Here, we further develop this method by introducing a technique for the expression of isotope-labeled glycoproteins by silkworm pupae, which has several potential advantages relative to larvae-based techniques in terms of production yield, ease of handling, and storage. Here, we fed fifth instar larvae an artificial diet with an optimized composition containing [methyl-13C]methionine, leading to pupation. Nine-day-old pupae were then injected with recombinant Bombyx mori nucleopolyhedrovirus (BmNPV) bacmid for expression of recombinant human immunoglobulin G (IgG). From the whole-body homogenates of pupae, 0.35 mg/pupa of IgG was harvested, which is a yield that is five times higher than can be obtained from larvae. Recombinant IgG, thus prepared, exhibited mainly three kinds of pauci-mannose-type oligosaccharides and had a 13C-enrichment ratio of approximately 80%. This enabled selective observation of NMR signals originating from the methionyl methyl group of IgG, confirming its conformational integrity. These data demonstrate the utility of silkworm pupae as factories for producing recombinant glycoproteins with amino-acid-selective isotope labeling.
- Published
- 2020
19. Suitable Combination Between Beauveria bassiana (Balsamo) Vuillemin and Four Plant Leaf Extracts to Control Spodoptera litura (Fabricius)
- Author
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Yogo Setiawan, Aminudin Afandhi, Mochammad Syamsul Hadi, Vivi Renna Pratiwi, and Retno Dyah Puspitarini
- Subjects
0106 biological sciences ,plant extracts ,Beauveria bassiana ,Lantana ,Spodoptera litura ,Bassiana ,compatibility ,lcsh:Plant culture ,01 natural sciences ,Conidium ,lcsh:Agriculture ,Insect pest ,lcsh:SB1-1110 ,larval mortality ,beauveria bassiana ,Larva ,biology ,fungi ,lcsh:S ,food and beverages ,biology.organism_classification ,Sunflower ,010602 entomology ,Horticulture ,conidial density and viability ,Agronomy and Crop Science ,010606 plant biology & botany - Abstract
Lepidopteran pests such as S. litura might cause yield losses in many crops especially during pest outbreak. The combinations of microbial and botanical insecticides were expected to increase the effectivity in controlling the respected insect pest. This research aimed to evaluate the combination of plant extracts and B. bassiana to control S. litura. The compatibility of B. bassiana with different plant extracts such as Neem, Chinaberry, Mexican Sunflower and Lantana leaves was studied in the laboratory. The compatibility was evaluated based on B. bassiana colony growth, conidia density, conidia viability, and mortality of S. litura larvae. After 12 days after application (DAA), colony growth of B. bassiana was reduced by all plant extract treatments. For the mortality of S. litura, the combined mixture of B. bassiana and 0.25% Chinaberry extract resulted the maximum mortality rate (44%). After 12 and 15 DAA, the 0.25% Chinaberry showed the highest colony growth (3.93 and 4.37 cm). The most suitable treatment was B. bassiana with 0.25% Chinaberry that had the conidial density at 1.77 x 108 conidia/ml and conidial viability of 75.63% and can enhanced the mortality of S. litura larvae.
- Published
- 2020
20. Biophysical characterization of dynamic structures of immunoglobulin G
- Author
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Koichi Kato, Saeko Yanaka, and Rina Yogo
- Subjects
chemistry.chemical_classification ,0303 health sciences ,biology ,Chemistry ,Effector ,Allosteric regulation ,Biophysics ,Membrane biology ,Review ,010402 general chemistry ,01 natural sciences ,Immunoglobulin G ,0104 chemical sciences ,03 medical and health sciences ,Molecular dynamics ,Structural Biology ,biology.protein ,Antibody ,Glycoprotein ,Receptor ,Molecular Biology ,030304 developmental biology - Abstract
Immunoglobulin G (IgG) is a major antibody and functions as a hub linking specific antigen binding and recruitment of effector molecules typified by Fcγ receptors (FcγRs). These activities are associated primarily with interactions involving its Fab and Fc sites, respectively. An IgG molecule is characterized by a multiple domain modular structure with conserved N-glycosylation in Fc. The molecule displays significant freedom in internal motion on various spatiotemporal scales. The consequent conformational flexibility and plasticity of IgG glycoproteins are functionally significant and potentially important factors for design and engineering of antibodies with enhanced functionality. In this article, experimental and computational approaches are outlined for characterizing the conformational dynamics of IgG molecules in solution. In particular, the importance of integration of these approaches is highlighted, as illustrated by dynamic intramolecular interactions between the pair of N-glycans and their proximal amino acid residues in Fc. These interactions can critically affect effector functions mediated by human IgG1 and FcγRIII. Further improvements in individual biophysical techniques and their integration will advance understanding of dynamic behaviors of antibodies in physiological and pathological conditions. Such understanding will provide opportunities for engineering antibodies through controlling allosteric networks in IgG molecules.
- Published
- 2020
21. SNAIL regulates gastric carcinogenesis through CCN3 and NEFL
- Author
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Shoko Yokoyama, Akihiko Yoshizawa, Akitada Yogo, Kenji Masuo, Shigeo Takaishi, Aiko Sugiyama, Ru Chen, and Hiroshi Seno
- Subjects
Cancer Research ,Epithelial-Mesenchymal Transition ,Carcinogenesis ,Down-Regulation ,Snail ,medicine.disease_cause ,Mice ,Nephroblastoma Overexpressed Protein ,Cancer stem cell ,Neurofilament Proteins ,Stomach Neoplasms ,biology.animal ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Cell Proliferation ,biology ,Microarray analysis techniques ,digestive, oral, and skin physiology ,CD44 ,Stomach ,General Medicine ,Xenograft Model Antitumor Assays ,Up-Regulation ,Gene Expression Regulation, Neoplastic ,Cell culture ,Drug Resistance, Neoplasm ,Gene Knockdown Techniques ,Cancer cell ,Cancer research ,biology.protein ,Neoplastic Stem Cells ,Fluorouracil ,Snail Family Transcription Factors ,Stem cell ,Signal Transduction - Abstract
Among cancer cells, there are specific cell populations of whose activities are comparable to those of stem cells in normal tissues, and for whom the levels of cell dedifferentiation are reported to correlate with poor prognosis. Information concerning the mechanisms that modulate the stemness like traits of cancer cells is limited. Therefore, we examined five gastric cancer cell lines and isolated gastric oncospheres from three gastric cancer cell lines. The gastric cancer cells that expanded in the spheres expressed relatively elevated proportion of CD44, which is a marker of gastric cancer stem cells (CSCs), and displayed many properties of CSCs, for example: chemoresistance, tumorigenicity and epithelial–mesenchymal transition (EMT) acquisition. SNAIL, which is a key factor in EMT, was highly expressed in the gastric spheres. Microarray analysis in gastric cancer cell line HGC27 showed that CCN3 and NEFL displayed the greatest differential expression by knocking down of SNAIL; the former was upregulated and the latter downregulated, respectively. Downregulation of CCN3 and upregulation of NEFL gene expression impaired the SNAIL-dependent EMT activity: high tumorigenicity, and chemoresistance in gastric cancer cells. Thus, approach that disrupts SNAIL/CCN3/NEFL axis may be credible in inhibiting gastric cancer development.
- Published
- 2020
22. Impacts of Zn and Cu enrichment under ocean acidification scenario on a phytoplankton community from tropical upwelling system
- Author
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Durbar Ray, Saumya Silori, Debasmita Bandyopadhyay, Haimanti Biswas, Damien Cardinal, Diksha Sharma, Magloire Mandeng-Yogo, Aziz Ur Rahman Shaik, CSIR National Institute of Oceanography [India] (NIO), Cycles biogéochimiques marins : processus et perturbations (CYBIOM), Laboratoire d'Océanographie et du Climat : Expérimentations et Approches Numériques (LOCEAN), Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-École normale supérieure - Paris (ENS-PSL), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité)-Muséum national d'Histoire naturelle (MNHN)-Institut de Recherche pour le Développement (IRD)-Institut national des sciences de l'Univers (INSU - CNRS)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université Paris Cité (UPCité), Variabilité à long terme du climat de l'océan (VALCO), Institut Pierre-Simon-Laplace (IPSL (FR_636)), École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-École normale supérieure - Paris (ENS Paris), Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)-Institut Pierre-Simon-Laplace (IPSL (FR_636)), and Université Paris sciences et lettres (PSL)-Université Paris sciences et lettres (PSL)-Université de Versailles Saint-Quentin-en-Yvelines (UVSQ)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Institut national des sciences de l'Univers (INSU - CNRS)-École polytechnique (X)-Centre National d'Études Spatiales [Toulouse] (CNES)-Sorbonne Université (SU)-Centre National de la Recherche Scientifique (CNRS)-Université de Paris (UP)-Institut de Recherche pour le Développement (IRD)-Muséum national d'Histoire naturelle (MNHN)-Centre National de la Recherche Scientifique (CNRS)-Sorbonne Université (SU)
- Subjects
0106 biological sciences ,Nitzschia ,India ,[PHYS.PHYS.PHYS-GEO-PH]Physics [physics]/Physics [physics]/Geophysics [physics.geo-ph] ,Aquatic Science ,Oceanography ,010603 evolutionary biology ,01 natural sciences ,Phytoplankton ,Seawater ,Trace metal ,Arabian sea ,14. Life underwater ,ComputingMilieux_MISCELLANEOUS ,Diatoms ,Upwelling ,biology ,Chemistry ,Ocean acidification ,010604 marine biology & hydrobiology ,fungi ,Chaetoceros ,General Medicine ,Hydrogen-Ion Concentration ,biology.organism_classification ,Pollution ,Zinc ,Diatom ,13. Climate action ,Environmental chemistry ,Bloom ,Acids ,Copper - Abstract
Increasing dissolution of CO2 in the surface ocean is rapidly decreasing its pH and changing carbon chemistry which is further affecting marine biota in several ways. Phytoplankton response studies under the combination of elevated CO2 and trace metals are rare. We have conducted two consecutive onboard incubation experiments (R. V. Sindhu Sadhana; August 2017) in the eastern Arabian Sea (SW coast of India) during an upwelling event. A nutrient enriched diatom bloom was initiated onboard and grown under ambient (approximate to 400 mu atm, A-CO2) and high CO 2 levels (approximate to 1000 patm; H-CO2) with different zinc (Zn; 1 nM) and copper (Cu) concentrations (1 nM, 2 nM and 8 nM). Phytoplankton community composition and the dominant genera were different during these two experiments. CO2 enrichment alone did not show any significant growth stimulating impact on the experimental community except enhanced cell density in the first experiment. Addition of Zn at A-CO2 level revealed no noticeable responses; whereas, the same treatment under H-CO2 level significantly reduced cell number. Considerably high protein content under H-CO2+Zn treatment was possibly counteracting Zn toxicity which also caused slower growth rate. Cu addition did not show any noticeable impact on growth and biomass production except increased protein content as well as decreased carbohydrate: protein ratio. This can be attributed to relatively higher protein synthesis than carbohydrate to alleviate oxidative stress generated by Cu. The centric diatom Chaetoceros and toxin producing pennate diatom Pseudo-nitzschia showed no significant response to either CO2 or Zn enrichment. Large centric diatom Leptocylindrus and Skeletonema responded positively to Zn addition in both CO2 levels. The former species showed the most sensitive response at the highest Cu and H-CO2 treatment; whereas, the pennate diatoms Mtzschia and Pseudo-nttzschia (toxigenic diatom) showed higher resilience under elevated CO2 and Cu levels. This observation indicated that in future ocean, increasing CO2 concentrations and trace metal pollution may potentially alter phytoplankton community structure and may facilitate toxigenic diatom bloom in the coastal waters.
- Published
- 2020
23. On-Membrane Dynamic Interplay between Anti-GM1 IgG Antibodies and Complement Component C1q
- Author
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Nobuhiro Yuki, Yuki Taniguchi, Hiroki Watanabe, Hiromune Ando, Saeko Yanaka, Naoko Komura, Koichi Kato, Hirokazu Yagi, Tadashi Satoh, Takayuki Uchihashi, and Rina Yogo
- Subjects
0301 basic medicine ,high-speed atomic force microscopy ,02 engineering and technology ,G(M1) Ganglioside ,protein A ,medicine.disease_cause ,Guillain-Barre Syndrome ,Microscopy, Atomic Force ,Guillain–Barré syndrome ,Catalysis ,Immunoglobulin G ,Article ,Inorganic Chemistry ,lcsh:Chemistry ,03 medical and health sciences ,immunoglobulin G ,Immune system ,medicine ,Humans ,Physical and Theoretical Chemistry ,Molecular Biology ,lcsh:QH301-705.5 ,Spectroscopy ,ganglioside GM1 ,Ganglioside ,biology ,Fc ,Chemistry ,Complement C1q ,Organic Chemistry ,Autoantibody ,General Medicine ,021001 nanoscience & nanotechnology ,Computer Science Applications ,Complement system ,Cell biology ,Molecular mimicry ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,biology.protein ,Antibody ,0210 nano-technology ,Protein A ,Protein Binding - Abstract
Guillain&ndash, Barré, syndrome, an autoimmune neuropathy characterized by acute limb weakness, is often preceded by Campylobacter jejuni infection. Molecular mimicry exists between the bacterial lipo-oligosaccharide and human ganglioside. Such C. jejuni infection induces production of immunoglobulin G1 (IgG1) autoantibodies against GM1 and causes complement-mediated motor nerve injury. For elucidating the molecular mechanisms linking autoantigen recognition and complement activation, we characterized the dynamic interactions of anti-GM1 IgG autoantibodies on ganglioside-incorporated membranes. Using high-speed atomic force microscopy, we found that the IgG molecules assemble into a hexameric ring structure on the membranes depending on their specific interactions with GM1. Complement component C1q was specifically recruited onto these IgG rings. The ring formation was inhibited by an IgG-binding domain of staphylococcal protein A bound at the cleft between the CH2 and CH3 domains. These data indicate that the IgG assembly is mediated through Fc&ndash, Fc interactions, which are promoted under on-membrane conditions due to restricted translational diffusion of IgG molecules. Reduction and alkylation of the hinge disulfide impaired IgG ring formation, presumably because of an increase in conformational entropic penalty. Our findings provide mechanistic insights into the molecular processes involved in Guillain&ndash, syndrome and, more generally, into antigen-dependent interplay between antibodies and complement components on membranes.
- Published
- 2019
24. Phytoplasma Detection on Sugarcane Crops in South Sulawesi
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Baharuddin Baharuddin, Nur Amin, and Catur Yogo Hendro Utomo
- Subjects
Agronomy ,Phytoplasma ,Biology ,biology.organism_classification - Published
- 2018
25. Hepatic Overexpression of Endothelial Lipase Lowers High-Density Lipoprotein but Maintains Reverse Cholesterol Transport in Mice
- Author
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Shunichi Takiguchi, Harumi Uto-Kondo, Makiko Yogo, Katsunori Ikewaki, Emi Yakushiji, Maki Iizuka, Kazuhiro Nakaya, Takafumi Nishida, Tomohiro Komatsu, Masatsune Ogura, Yoshio Terao, Makoto Sasaki, and Makoto Ayaori
- Subjects
high-density lipoproteins ,0301 basic medicine ,Endothelial lipase ,medicine.medical_specialty ,Genetic Vectors ,Probucol ,ATP-binding cassette transporter 1 ,030204 cardiovascular system & hematology ,Adenoviridae ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,High-density lipoprotein ,Internal medicine ,medicine ,Animals ,Humans ,Scavenger receptor ,biology ,Basic Sciences ,Cholesterol ,Cholesterol, HDL ,Reverse cholesterol transport ,Gene Transfer Techniques ,Hep G2 Cells ,Lipase ,Scavenger Receptors, Class B ,endothelial lipase ,Mice, Inbred C57BL ,MicroRNAs ,RAW 264.7 Cells ,030104 developmental biology ,Endocrinology ,Liver ,chemistry ,Enzyme Induction ,ABCA1 ,ComputingMethodologies_DOCUMENTANDTEXTPROCESSING ,Macrophages, Peritoneal ,biology.protein ,RNA Interference ,lipids (amino acids, peptides, and proteins) ,Cardiology and Cardiovascular Medicine ,ATP Binding Cassette Transporter 1 ,Lipoprotein ,medicine.drug - Abstract
Supplemental Digital Content is available in the text., Objective— Reverse cholesterol transport (RCT) is a major mechanism by which HDL (high-density lipoprotein) protects against atherosclerosis. Endothelial lipase (EL) reportedly reduces HDL levels, which, in theory, would increase atherosclerosis. However, it remains unclear whether EL affects RCT in vivo. Approach and Results— Adenoviral vectors expressing EL or luciferase were intravenously injected into mice, and a macrophage RCT assay was performed. As expected, hepatic EL overexpression markedly reduced HDL levels. In parallel, plasma 3H-cholesterol counts from the EL-expressing mice decreased by 85% compared with control. Surprisingly, there was no difference in fecal 3H-cholesterol excretion between the groups. Kinetic studies revealed increased catabolism/hepatic uptake of 3HDL-cholesteryl ether, resulting in no change in fecal HDL-cholesteryl ester excretion in the mice. To explore underlying mechanisms for the preservation of RCT despite low HDL levels in the EL-expressing mice, we investigated the effects of hepatic SR-BI (scavenger receptor class B type I) knockdown. RCT assay revealed that knockdown of SR-BI alone reduced fecal excretion of macrophage-derived 3H-cholesterol. Interestingly, hepatic EL overexpression under SR-BI inhibition further attenuated fecal tracer counts as compared with control. Finally, we observed that EL overexpression enhanced in vivo RCT under pharmacological inhibition of hepatic ABCA1 (ATP-binding cassette transporter A1) by probucol. Conclusions— Hepatic EL expression compensates for reduced macrophage-derived cholesterol efflux to plasma because of low HDL levels by promoting cholesterol excretion to bile/feces via an SR-BI pathway, maintaining overall RCT in vivo. In contrast, EL-modified HDL might negatively regulate RCT via hepatic ABCA1. Despite extreme hypoalphalipoproteinemia, RCT is maintained in EL-expressing mice via SR-BI/ABCA1-dependent pathways.
- Published
- 2018
26. Anti-IL-6 receptor antibody improves pain symptoms in mice with experimental autoimmune encephalomyelitis
- Author
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Yoshihiro Matsumoto, Mayu Magi, Haruna Tomizawa-Shinohara, Kenji Yogo, and Kenichi Serizawa
- Subjects
Pain Threshold ,0301 basic medicine ,Encephalomyelitis, Autoimmune, Experimental ,Immunology ,Pain ,Myelin oligodendrocyte glycoprotein ,Mice ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Animals ,Immunology and Allergy ,Nociception assay ,Analgesics ,Neuromyelitis optica ,biology ,business.industry ,Multiple sclerosis ,Experimental autoimmune encephalomyelitis ,Chronic pain ,medicine.disease ,Spinal cord ,Receptors, Interleukin-6 ,Mice, Inbred C57BL ,030104 developmental biology ,medicine.anatomical_structure ,Neurology ,Hyperalgesia ,Neuropathic pain ,biology.protein ,Female ,Microglia ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
Background and aims Chronic pain is a prevalent symptom in patients with autoimmune encephalomyelitis such as multiple sclerosis and neuromyelitis optica. Although IL-6 is involved in various inflammatory and immune diseases, the roles of IL-6 in autoimmune-related pain have not been clarified. Therefore, we examined the effect of anti-IL-6 receptor antibody (MR16-1) on the pain sensitivity of experimental autoimmune encephalomyelitis (EAE) mice. Materials and methods EAE was induced in female C57BL/6J mice by subcutaneous immunization with myelin oligodendrocyte glycoprotein 35–55 emulsified in adjuvant (Day 0). Pertussis toxin was intravenously administered at Days 0 and 2. Mice were sequentially scored for clinical symptoms of EAE. [Exp. 1] MR16-1 was intraperitoneally administered on Days 0 or 3. Sensitivity to pain was measured by the von Frey test (Days 7, 14, 20). The spinal cord was isolated and assessed by immunohistochemistry. [Exp. 2] MR16-1 was intraperitoneally administered on Day 12 when significant pain had already occurred. Pain assessment was conducted before the immunization, on Day 12 and after EAE onset. And then, spinal cord was isolated and flow cytometry was performed. Results [Exp. 1] MR16-1 prevented the increase in clinical score and sensitivity to pain in EAE mice. Immunohistochemical analysis showed that Iba1 + microglia were increased in the spinal cord of EAE mice, and were reduced by MR16-1. [Exp. 2] Administration of MR16-1 on Day 12 also reduced sensitivity to pain under EAE onset. Flow cytometry showed that CD45 low CD11b + microglia were increased in the spinal cord of EAE mice, and that this increase was inhibited by MR16-1. Conclusion These findings suggest that MR16-1 can decrease mechanical allodynia in EAE mice through inhibition of microglial activation and proliferation in the spinal cord.
- Published
- 2018
27. eIF2 β, a subunit of translation-initiation factor EIF2, is a potential therapeutic target for non-small cell lung cancer
- Author
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Tetsunari Hase, Naoyuki Yogo, Masahiro Morise, Lauren Averett Byers, Yoshinori Hasegawa, Tomohiko Kakumu, John D. Minna, Daiki Goto, Mitsuo Sato, Toshio Kato, Masashi Kondo, Ichidai Tanaka, Kevin R. Coombes, John V. Heymach, Ayako Miyazawa, Yoshitaka Sekido, and Luc Girard
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Lung Neoplasms ,Eukaryotic Initiation Factor-2 ,Kaplan-Meier Estimate ,Adenocarcinoma ,Biology ,Cell Line ,Small hairpin RNA ,03 medical and health sciences ,0302 clinical medicine ,RNA interference ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Gene expression ,medicine ,Humans ,Molecular Targeted Therapy ,Lung cancer ,Gene ,Aged ,A549 cell ,General Medicine ,Middle Aged ,medicine.disease ,respiratory tract diseases ,Gene Expression Regulation, Neoplastic ,Protein Subunits ,030104 developmental biology ,Oncology ,A549 Cells ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Female ,RNA Interference - Abstract
To identify novel therapeutic targets for non-small cell lung cancer (NSCLC), we conducted an integrative study in the following 3 stages: (i) identification of potential target gene(s) through shRNA functional screens in 2 independent NSCLC cell lines; (ii) validation of the clinical relevance of identified gene(s) using public databases; and (iii) investigation of therapeutic potential of targeting the identified gene(s) in vitro. A semi-genome-wide shRNA screen was performed in NCI-H358 cells, and was integrated with data from our previous screen in NCI-H460 cells. Among genes identified in shRNA screens, 24 were present in both NCI-H358 and NCI-H460 cells and were considered potential targets. Among the genes, we focused on eIF2β, which is a subunit of heterotrimeric G protein EIF2 and functions as a transcription initiation factor. The eIF2β protein is highly expressed in lung cancer cell lines compared with normal bronchial epithelial cells, and gene copy number analyses revealed that eIF2β is amplified in a subset of NSCLC cell lines. Gene expression analysis using The Cancer Genome Atlas (TCGA) dataset revealed that eIF2β expression is significantly upregulated in lung cancer tissues compared with corresponding normal lung tissues. Furthermore, high eIF2β expression was correlated with poor survival in patients with lung adenocarcinoma, as shown in other cohorts using publicly available online tools. RNAi-mediated depletion of eIF2β suppresses growth of lung cancer cells independently of p53 mutation status, in part through G1 cell cycle arrest. Our data suggest that eIF2β is a therapeutic target for lung cancer.
- Published
- 2018
28. Backbone 1H, 13C, and 15N assignments of the extracellular region of human Fcγ receptor IIIb
- Author
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Rina Yogo, Koichi Kato, and Saeko Yanaka
- Subjects
0301 basic medicine ,biology ,Chemistry ,Phagocytosis ,Degranulation ,Complement receptor ,Biochemistry ,Immunoglobulin G ,03 medical and health sciences ,030104 developmental biology ,Immune system ,Structural Biology ,biology.protein ,Extracellular ,Receptor ,Opsonin - Abstract
Fcγ receptor (FcγR) promotes various immune responses through interactions with the Fc portion of immunoglobulin G (IgG). FcγRIIIb is a glycosylphosphatidylinositol-linked protein expressed on neutrophils and triggers degranulation and opsonic phagocytosis. The extracellular region of FcγR is composed of two Ig-fold domains and can be cleaved as a soluble form (sFcγRIIIb), which is also reactive with complement receptor type 3. Although structure and Fc interaction of sFcγRIIIb have been characterized by X-ray crystallography, little has been known about its structure in solution. We herein report the backbone NMR assignments of human sFcγRIIIb to gain basic understanding of functional IgG-FcγRIII interactions of immunological and biopharmaceutical interest regarding the structural investigation.
- Published
- 2018
29. Development of an Activatable Fluorescent Probe for Prostate Cancer Imaging
- Author
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Keitaro Umezawa, Mako Kamiya, Takao Yogo, Rumi Hino, and Yasuteru Urano
- Subjects
Male ,0301 basic medicine ,Fluorescence-lifetime imaging microscopy ,Proteases ,Pathology ,medicine.medical_specialty ,Hepsin ,Biomedical Engineering ,Glutamic Acid ,Pharmaceutical Science ,Bioengineering ,Mice ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Cell Line, Tumor ,medicine ,Animals ,Humans ,Matriptase ,Fluorescent Dyes ,Pharmacology ,biology ,Rhodamines ,Chemistry ,Optical Imaging ,Serine Endopeptidases ,Organic Chemistry ,Prostatic Neoplasms ,Cancer ,medicine.disease ,Fluorescence ,In vitro ,030104 developmental biology ,030220 oncology & carcinogenesis ,biology.protein ,Biotechnology - Abstract
Technology to visualize small prostate cancers is urgently needed because of the difficulty of discriminating prostate cancer from normal tissue with the naked eye, and a fluorescence imaging method would be advantageous. Here, we describe the design and synthesis of a fluorogenic probe (Ac-KQLR-HMRG) that is activated by hepsin and matriptase (proteases over-expressed in prostate cancer). Ac-KQLR-HMRG exhibited significant turn-on fluorogenicity in the presence of hepsin (180-fold) and matriptase (80-fold) and allowed specific fluorescence imaging of various prostate cancer cell line in vitro. In addition, the probe enabled rapid imaging (within 1-10 min) of small prostate cancer nodules in mouse models of disseminated peritoneal tumor and orthotopic tumor.
- Published
- 2017
30. Identification of proteasomal catalytic subunit PSMA6 as a therapeutic target for lung cancer
- Author
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Masashi Kondo, Tomohiko Kakumu, Kohei Yokoi, Yoshitaka Sekido, Tetsunari Hase, Naoyuki Yogo, Lauren Averett Byers, John V. Heymach, John D. Minna, Mitsuo Sato, Masahiro Morise, Toshio Kato, Luc Girard, Yoshinori Hasegawa, Kevin R. Coombes, Daiki Goto, and Takayuki Fukui
- Subjects
0301 basic medicine ,Male ,Cancer Research ,Candidate gene ,Spliceosome ,Proteasome Endopeptidase Complex ,Lung Neoplasms ,Cell Survival ,Blotting, Western ,PSMA6 ,Apoptosis ,Kaplan-Meier Estimate ,Biology ,Cell Line ,Small hairpin RNA ,03 medical and health sciences ,Cell, Molecular, and Stem Cell Biology ,Carcinoma, Non-Small-Cell Lung ,Catalytic Domain ,Cell Line, Tumor ,medicine ,Gene silencing ,Humans ,Molecular Targeted Therapy ,Lung cancer ,Gene ,Aged ,Cell Proliferation ,spliceosomes ,Reverse Transcriptase Polymerase Chain Reaction ,Gene Expression Profiling ,Gene Amplification ,General Medicine ,Original Articles ,medicine.disease ,small interfering RNA ,Immunohistochemistry ,3. Good health ,G2 Phase Cell Cycle Checkpoints ,Gene Expression Regulation, Neoplastic ,030104 developmental biology ,Oncology ,Proteasome ,A549 Cells ,Cancer research ,Original Article ,Female ,RNA Interference ,Signal Transduction - Abstract
To identify potential therapeutic targets for lung cancer, we performed semi-genome-wide shRNA screening combined with the utilization of genome-wide expression and copy number data. shRNA screening targeting 5043 genes in NCI-H460 identified 51 genes as candidates. Pathway analysis revealed that the 51 genes were enriched for the five pathways, including ribosome, proteasome, RNA polymerase, pyrimidine metabolism and spliceosome pathways. We focused on the proteasome pathway that involved six candidate genes because its activation has been demonstrated in diverse human malignancies, including lung cancer. Microarray expression and array CGH data showed that PSMA6, a proteasomal subunit of a 20S catalytic core complex, was highly expressed in lung cancer cell lines, with recurrent gene amplifications in some cases. Therefore, we further examined the roles of PSMA6 in lung cancer. Silencing of PSMA6 induced apoptosis or G2/M cell cycle arrest in cancer cell lines but not in an immortalized normal lung cell line. These results suggested that PSMA6 serves as an attractive target with a high therapeutic index for lung cancer.
- Published
- 2017
31. Production of an active form of vitamin D 2 by genetically engineered CYP105A1
- Author
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Yuya Yogo, Miho Ohta, Kiyoshi Yasukawa, Masaki Kamakura, Yoshitsugu Shiro, Hiroshi Sugimoto, Toshiyuki Sakaki, Kaori Yasuda, Shinichi Ikushiro, Hiroki Mano, and Teisuke Takita
- Subjects
0301 basic medicine ,chemistry.chemical_classification ,Vitamin ,030102 biochemistry & molecular biology ,biology ,Chemistry ,Stereochemistry ,Biophysics ,Wild type ,Cytochrome P450 ,Cell Biology ,Protein engineering ,Biochemistry ,Hydroxylation ,03 medical and health sciences ,chemistry.chemical_compound ,030104 developmental biology ,Docking (molecular) ,Oxidoreductase ,biology.protein ,Side chain ,Molecular Biology - Abstract
Our previous studies revealed that CYP105A1 can convert vitamin D3 (VD3) to its active form, 1α,25-dihydroxyvitamin D3 (1,25D3). Site-directed mutagenesis of CYP105A1 based on its crystal structure dramatically enhanced its activity; the activity of double variants R73A/R84A and R73A/R84V was more than 100-fold higher than that of the wild type of CYP105A1. In contrast, these variants had a low ability to convert vitamin D2 (VD2) to 1α,25-dihydroxyvitamin D2 (1,25D2), whereas they catalyzed the sequential hydroxylation at positions C25 and C26 to produce 25,26D2. A comparison of the docking models of 25D2 and 25D3 into the substrate-binding pocket of R73A/R84A suggests that the side chain of the Met239 inhibits the binding of 25D2 for 1α-hydroxylation. Therefore, the Met239 residue of R73A/R84A was substituted for Ala. As expected, the triple variant R73A/R84A/M239A showed a 22-fold higher 1α-hydroxylation activity towards 25D2. To the best of our knowledge, this is the first report on the generation of microbial cytochrome P450 that converts VD2 to 1,25D2 via 25D2.
- Published
- 2017
32. Study on the Safety and Efficacy of Indonesian Combination Plant Extract in Cosmetics
- Author
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Kilala Tilaar, Maily, Okmadanti Citrasari, Yogo Suro Priyadi, Martha Tilaar, Diah Puspitosari, and Devi Junardy
- Subjects
Active ingredient ,Antioxidant ,Centella ,Traditional medicine ,biology ,business.industry ,medicine.medical_treatment ,Patch test ,Human skin ,medicine.disease_cause ,Ascorbic acid ,biology.organism_classification ,chemistry.chemical_compound ,chemistry ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Irritation ,business ,Kojic acid - Abstract
Objective: To look for antioxidant, lightening and anti aging effects from combination of Indonesian plant consist of Centella asiatica, Camellia sinensis and Glycine max extract in propanediol (CCGE). Methodology: The study was carried out using DPPH scavenging activity for antioxidant effect and anti tyrosinase activity for lightening effect. The efficacy test on volunteers was performed using Mexameter MX 16 and Cutometer MPA 580 to measure skin melanin index, elasticity and firmness respectively. The dermatological safety test was conducted using Repeated Opened Patch Test (ROPT) and Single Closed Patch Test (SCPT), while HETCAM test was performed to evaluate irritation at ocular level. Result: At the concentration of 2%, CCGE has DPPH scavenging activity and anti tyrosinase activity of 70.56% and 52.76% respectively compared to ascorbic acid and kojic acid. The efficacy test showed that CCGE significantly increased skin elasticity and firmness after two and four weeks of application compared to base, but showed no significant effect on skin lightening. The dermatological safety test showed that CCGE did not cause any irritation or allergic skin reaction at the concentration of 5% for the ROPT and up to 3% for the SCPT. Meanwhile at the concentration of 5%, CCGE could cause allergic reaction in 4.0% of all subjects. The HETCAM test showed that CCGE is not irritant at the ocular level. Conclusion: These results concluded that CCGE is proven to be safe for human skin as well as at ocular level and could be used as cosmetic active ingredient for anti aging. Key words: Centella asiatica, Camellia sinensis, Glycine max, Antioxidant, Lightening, Anti aging, Cosmetic.
- Published
- 2017
33. Plastid-targeted forms of restriction endonucleases enhance the plastid genome rearrangement rate and trigger the reorganization of its genomic architecture
- Author
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Nobuhiko Muramoto, Ritsuko Kitagawa‐Yogo, Minoru Hirano, Hidenori Tanaka, Norihiro Mitsukawa, Tanaka Tomoko, and Hiroki Sugimoto
- Subjects
0106 biological sciences ,0301 basic medicine ,Genome evolution ,DNA Copy Number Variations ,Genome, Plastid ,Plant Science ,Biology ,01 natural sciences ,Genome ,Evolution, Molecular ,03 medical and health sciences ,Arabidopsis ,Genetics ,Copy-number variation ,Plastids ,Plastid ,Genome, Chloroplast ,Gene ,Variegation ,food and beverages ,Cell Biology ,DNA Restriction Enzymes ,biology.organism_classification ,Restriction enzyme ,030104 developmental biology ,010606 plant biology & botany - Abstract
Plant cells have acquired chloroplasts (plastids) with a unique genome (ptDNA), which developed during the evolution of endosymbiosis. The gene content and genome structure of ptDNAs in land plants are considerably stable, although those of algal ptDNAs are highly varied. Plant cells seem, therefore, to be intolerant of any structural or organizational changes in the ptDNA. Genome rearrangement functions as a driver of genomic evolutionary divergence. Here, we aimed to create various types of rearrangements in the ptDNA of Arabidopsis genomes using plastid-targeted forms of restriction endonucleases (pREs). Arabidopsis plants expressing each of the three specific pREs, i.e., pTaqI, pHinP1I, and pMseI, were generated; they showed the leaf variegation phenotypes associated with impaired chloroplast development. We confirmed that these pREs caused double-stranded breaks (DSB) at their recognition sites in ptDNAs. Genome-wide analysis of ptDNAs revealed that the transgenic lines exhibited a large number of rearrangements such as inversions and deletions/duplications, which were dominantly repaired by microhomology-mediated recombination and microhomology-mediated end-joining, and less by non-homologous end-joining. Notably, pHinP1I, which recognized a small number of sites in ptDNA, induced drastic structural changes, including regional copy number variations throughout ptDNAs. In contrast, the transient expression of either pTaqI or pMseI, whose recognition site numbers were relatively larger, resulted in small-scale changes at the whole genome level. These results indicated that DSB frequencies and their distribution are major determinants in shaping ptDNAs.
- Published
- 2019
34. Changes in canine C-reactive protein levels following orthopaedic surgery: a prospective study
- Author
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Noriyuki Hayakawa, Yasuji Harada, Yasushi Hara, Takuya Yogo, Nobuo Kanno, and Shuji Suzuki
- Subjects
medicine.medical_specialty ,040301 veterinary sciences ,Brief Communication ,0403 veterinary science ,Sepsis ,Clinical study ,Postoperative complications ,Visceral disease ,Dogs ,Surgical site ,medicine ,Orthopaedic procedures ,Animals ,Orthopedic Procedures ,Postoperative Period ,Prospective Studies ,Prospective cohort study ,Inflammation ,lcsh:Veterinary medicine ,General Veterinary ,biology ,business.industry ,Canine inflammatory biomarker ,C-reactive protein ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,General Medicine ,medicine.disease ,040201 dairy & animal science ,C-Reactive Protein ,Anesthesia ,Orthopedic surgery ,biology.protein ,lcsh:SF600-1100 ,Female ,business ,Biomarkers ,Blood sampling - Abstract
C-reactive protein (CRP) is a powerful biomarker for inflammation, infection and sepsis. However, no reports have investigated canine CRP (c-CRP) concentration changes after orthopaedic procedures. If c-CRP changes were better characterized, it may be possible to identify postoperative complications more quickly. The purpose of this study was to clarify the characteristic changes in serum c-CRP after orthopaedic surgery in dogs. Blood samples were collected from 98 dogs on Day 0 (preoperatively), and then on Days 1, 4, 7, 10 and 13 postoperatively. Day 1 blood sampling was performed 12–24 h postoperatively. We classified the dogs into four groups based on changes in c-CRP pre- to postoperatively. Group 1 dogs showed a peak c-CRP concentration on Day 1, followed by decreases of ≥ 1 mg/dL. Group 2 dogs showed changes in c-CRP concentration by Day 4 that were within ± 1 mg/dL compared with Day 1. Dogs in Group 3 showed a peak c-CRP concentration on Day 4, followed by decreases of ≥ 1 mg/dL. Group 4 dogs showed an initial decrease in c-CRP, then an increase of ≥ 1 mg/dL. Group 1 was the largest group, with 63 dogs. c-CRP on Days 0, 1, 4, 7, 10 and 13 was 0.83 ± 1.03 mg/dL, 8.10 ± 3.15 mg/dL, 3.76 ± 1.94 mg/dL, 1.63 ± 0.92 mg/dL, 0.96 ± 0.70 mg/dL and 0.68 ± 0.51 mg/dL, respectively. Serum c-CRP concentration on Day 1 was significantly higher than at every other timepoint (P
- Published
- 2019
35. The Fab portion of immunoglobulin G contributes to its binding to Fcγ receptor III
- Author
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Masayoshi Onitsuka, Daisuke Higo, Rina Yogo, Takeshi Omasa, Mahito Nakanishi, Hiroki Watanabe, Yuki Yamaguchi, Shio Watanabe, Tetsuo Torisu, Mari Shimada, Saeko Yanaka, Takayuki Uchihashi, Susumu Uchiyama, Takahiro Maruno, Koichi Kato, Hirokazu Yagi, and Tadashi Satoh
- Subjects
0301 basic medicine ,Fc receptor ,lcsh:Medicine ,CHO Cells ,Immunoglobulin G ,Article ,03 medical and health sciences ,Immunoglobulin Fab Fragments ,Atomic force microscopy ,0302 clinical medicine ,Immune system ,Cricetulus ,Animals ,Humans ,lcsh:Science ,Receptor ,Multidisciplinary ,biology ,Chemistry ,Chinese hamster ovary cell ,lcsh:R ,Receptors, IgG ,biology.organism_classification ,Molecular biophysics ,Cell biology ,Immunoglobulin Fc Fragments ,030104 developmental biology ,biology.protein ,lcsh:Q ,Hydrogen–deuterium exchange ,Antibody ,Rituximab ,030217 neurology & neurosurgery - Abstract
Most cells active in the immune system express receptors for antibodies which mediate a variety of defensive mechanisms. These receptors interact with the Fc portion of the antibody and are therefore collectively called Fc receptors. Here, using high-speed atomic force microscopy, we observe interactions of human, humanized, and mouse/human-chimeric immunoglobulin G1 (IgG1) antibodies and their cognate Fc receptor, FcγRIIIa. Our results demonstrate that not only Fc but also Fab positively contributes to the interaction with the receptor. Furthermore, hydrogen/deuterium exchange mass spectrometric analysis reveals that the Fab portion of IgG1 is directly involved in its interaction with FcγRIIIa, in addition to the canonical Fc-mediated interaction. By targeting the previously unidentified receptor-interaction sites in IgG-Fab, our findings could inspire therapeutic antibody engineering.
- Published
- 2019
36. Combination of postoperative C-reactive protein value and computed tomography imaging can predict severe pancreatic fistula after pancreatoduodenectomy
- Author
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Toshihiko Masui, Kyoichi Takaori, Kazuyuki Nagai, Yuichiro Uchida, Tomoaki Yoh, Kenzo Nakano, Akitada Yogo, Takayuki Anazawa, and Shinji Uemoto
- Subjects
Adult ,Male ,medicine.medical_specialty ,Computed tomography ,030230 surgery ,Severity of Illness Index ,Pancreaticoduodenectomy ,03 medical and health sciences ,Pancreatic Fistula ,Young Adult ,0302 clinical medicine ,Postoperative Complications ,Predictive Value of Tests ,Risk Factors ,Medicine ,Humans ,Aged ,Retrospective Studies ,Aged, 80 and over ,Hepatology ,biology ,medicine.diagnostic_test ,business.industry ,C-reactive protein ,Gastroenterology ,Perioperative ,Middle Aged ,medicine.disease ,C-Reactive Protein ,ROC Curve ,Pancreatic fistula ,030220 oncology & carcinogenesis ,Amylases ,biology.protein ,Drainage ,Female ,Radiology ,business ,Tomography, X-Ray Computed - Abstract
Recent management after pancreatoduodenectomy recommends either omission of prophylactic drainage or early removal. This potentially makes the diagnosis of postoperative pancreatic fistula (POPF) difficult because the diagnosis is based on the amylase value of drain effluent. The aim of this study was to determine if severe POPF could be predicted independent of drainage information.Records of consecutive patients who underwent pancreatoduodenectomy between 2012 and 2018 were included for further analysis. The presence of a peripancreatic collection (PC) on routine postoperative (day7) computed tomography (early CT) and perioperative characteristics were analyzed.PC appeared in 82/211 patients (39%) and was associated with clinically relevant POPF (p 0.001). The C-reactive protein (CRP) on postoperative day5 was a good predictor of severe POPF (needing interventional therapy or Grade C) (area under the receiver operating characteristics curve, 0.802; 95% confidence interval, 0.702-0.875). Presence of a PC and a high CRP value were independent risk factors for severe POPF following multivariate analysis. The combination of CRP5.0 mg/dL on postoperative day 5 and the absence of a PC had 98% negative predictive value.The combination of CRP measurement and PC evaluation by early CT was useful in predicting severe POPF after pancreatoduodenectomy.
- Published
- 2019
37. Anti-IL-6 Receptor Antibody Inhibits Spontaneous Pain at the Pre-onset of Experimental Autoimmune Encephalomyelitis in Mice
- Author
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Hideyuki Yasuno, Yoshihiro Matsumoto, Haruna Tomizawa-Shinohara, Kenichi Serizawa, and Kenji Yogo
- Subjects
0301 basic medicine ,experimental autoimmune encephalomyelitis ,neuromyelitis optica ,multiple sclerosis ,Periaqueductal gray ,lcsh:RC346-429 ,Myelin oligodendrocyte glycoprotein ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,lcsh:Neurology. Diseases of the nervous system ,neuropathic pain ,IL-6 ,Neuromyelitis optica ,biology ,business.industry ,Multiple sclerosis ,anti-IL-6 receptor antibody ,spontaneous pain ,Experimental autoimmune encephalomyelitis ,Chronic pain ,Brief Research Report ,medicine.disease ,030104 developmental biology ,Neurology ,Neuropathic pain ,Immunology ,biology.protein ,Anti-IL-6 ,Neurology (clinical) ,business ,030217 neurology & neurosurgery - Abstract
Chronic pain is a significant symptom in patients with autoimmune encephalomyelitis, such as multiple sclerosis and neuromyelitis optica. The most commonly used animal model of these diseases is experimental autoimmune encephalomyelitis (EAE). We previously reported that evoked pain, such as mechanical allodynia, was improved by an anti-IL-6 receptor antibody in EAE mice. However, few reports have evaluated spontaneous pain in EAE mice. Here, we assessed spontaneous pain in EAE mice by utilizing the Mouse Grimace Scale (MGS, a standardized murine facial expression-based coding system) and evaluated the influence of an anti-IL-6 receptor antibody (MR16-1). EAE was induced in female C57BL/6J mice by subcutaneous immunization with myelin oligodendrocyte glycoprotein 35-55 emulsified in adjuvant and administration of pertussis toxin. Mice were placed individually in cubicles and filmed for about 10 min. Ten clear head shots per mouse from the video recording were given a score of 0, 1, or 2 for each of three facial action units: orbital tightening, nose bulge, and ear position. Clinical symptoms of EAE were also scored. Measurement of 5-HT in the spinal cord and functional imaging of the periaqueductal gray (PAG) were also performed. Compared with control mice, MGS score was significantly higher in EAE mice. MR16-1 prevented this increase, especially in pre-onset EAE mice. Promotion of spinal 5-HT turnover and reduction of PAG activity were observed in pre-onset EAE mice. These results suggest that MR16-1 prevented spontaneous pain developed before EAE onset.
- Published
- 2019
38. Sequential hydroxylation of vitamin D 2 by a genetically engineered CYP105A1
- Author
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Kiyoshi Yasukawa, Shinichi Ikushiro, Miho Ohta, Yuya Yogo, Teisuke Takita, Masaki Kamakura, Keiko Hayashi, Toshiyuki Sakaki, and Kaori Yasuda
- Subjects
0301 basic medicine ,Vitamin ,Stereochemistry ,Biophysics ,Hydroxylation ,Protein Engineering ,Biochemistry ,Mixed Function Oxygenases ,03 medical and health sciences ,chemistry.chemical_compound ,Bacterial Proteins ,Cytochrome P-450 Enzyme System ,Vitamin D and neurology ,medicine ,Molecular Biology ,chemistry.chemical_classification ,030102 biochemistry & molecular biology ,biology ,Wild type ,Cytochrome P450 ,Biological activity ,Cell Biology ,Amino acid ,Enzyme Activation ,Ergocalciferol ,030104 developmental biology ,chemistry ,Ergocalciferols ,biology.protein ,medicine.drug - Abstract
Our previous studies revealed that the double variants of CYP105A1- R73A/R84A and R73V/R84A-show high levels of activity with respect to conversion of vitamin D3 to its biologically active form, 1α,25-dihydroxyvitamin D3 (1α,25(OH)2D3). In this study, we found that both the double variants were also capable of converting vitamin D2 to its active form, that is, 1α,25-dihydroxyvitamin D2 (1α,25(OH)2D2), via 25(OH)D2, whereas its 1α-hydroxylation activity toward 25(OH)D2 was much lower than that toward 25(OH)D3. Comparison of the wild type and the double variants revealed that the amino acid substitutions remarkably enhanced both 25- and 26-hydroxylation activity toward vitamin D2. After 25-hydroxylation of vitamin D2, further hydroxylation at C26 may occur frequently without the release of 25(OH)D2 from the substrate-binding pocket. Thus, the double variants of CYP105A1 are quite useful to produce 25,26(OH)2D2 that is one of the metabolites of vitamin D2 detected in human serum.
- Published
- 2016
39. Probucol-Oxidized Products, Spiroquinone and Diphenoquinone, Promote Reverse Cholesterol Transport in Mice
- Author
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Kazusa Shiotani, Katsunori Ikewaki, Makoto Sasaki, Makiko Yogo, Shunichi Takiguchi, Takafumi Nishida, Shinji Yokoyama, Tomohiro Komatsu, Rui Lu, Masatsune Ogura, Harumi Uto-Kondo, Emi Yakushiji, Kazuhiro Nakaya, and Makoto Ayaori
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Time Factors ,Apolipoprotein B ,Hypercholesterolemia ,Aortic Diseases ,Probucol ,030204 cardiovascular system & hematology ,Apolipoproteins E ,Feces ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Internal medicine ,medicine ,Animals ,Aorta ,Mice, Knockout ,Apolipoprotein A-I ,biology ,Cholesterol ,Anticholesteremic Agents ,Macrophages ,Cholesterol, HDL ,Reverse cholesterol transport ,Quinones ,Biological Transport ,Transporter ,Atherosclerosis ,Plaque, Atherosclerotic ,Androstadienes ,Mice, Inbred C57BL ,Disease Models, Animal ,RAW 264.7 Cells ,030104 developmental biology ,Endocrinology ,ATP Binding Cassette Transporter 1 ,Liver ,chemistry ,biology.protein ,Cardiology and Cardiovascular Medicine ,Oxidation-Reduction ,medicine.drug ,Lipoprotein - Abstract
Objective— Oxidized products of probucol, spiroquinone and diphenoquinone, were shown to increase cell cholesterol release and plasma high-density lipoprotein (HDL) by inhibiting degradation of ATP-binding cassette transporter A1. We investigated whether these compounds enhance reverse cholesterol transport in mice. Approach and Results— Spiroquinone and diphenoquinone increased ATP-binding cassette transporter A1 protein (2.8- and 2.6-fold, respectively, P P P P P 3 H-cholesterol intraperitoneally into mice. Both spiroquinone and diphenoquinone increased fecal excretion of the macrophage-derived 3 H-tracer, by 25% and 28% ( P P 3 H-cholesterol, implying that macrophage-to-plasma was the most important step in spiroquinone/diphenoquinone-mediated promotion of in vivo reverse cholesterol transport. Finally, spiroquinone significantly reduced aortic atherosclerosis in apolipoprotein E null mice when compared with the vehicle. Conclusions— Spiroquinone and diphenoquinone increase functional ATP-binding cassette transporter A1 in both the macrophages and the liver, elevate plasma HDL-cholesterol, and promote overall reverse cholesterol transport in vivo. These compounds are promising as therapeutic reagents against atherosclerosis.
- Published
- 2016
40. Eldecalcitol prevents endothelial dysfunction in postmenopausal osteoporosis model rats
- Author
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Kenji Yogo, Koichi Endo, Ryohei Kawasaki, Ken Aizawa, Kenichi Serizawa, Satoshi Takeda, and Yoshihito Tashiro
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Nitric Oxide Synthase Type III ,Bone density ,Ovariectomy ,Endocrinology, Diabetes and Metabolism ,Peroxisome proliferator-activated receptor ,Vasodilation ,030204 cardiovascular system & hematology ,Rats, Sprague-Dawley ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Bone Density ,Bone Marrow ,Internal medicine ,medicine ,Animals ,Humans ,Vitamin D ,Endothelial dysfunction ,Cells, Cultured ,Osteoporosis, Postmenopausal ,Ultrasonography ,chemistry.chemical_classification ,biology ,business.industry ,Nitrotyrosine ,Eldecalcitol ,medicine.disease ,Rats ,Femoral Artery ,PPAR gamma ,Postmenopause ,Nitric oxide synthase ,030104 developmental biology ,chemistry ,Ovariectomized rat ,biology.protein ,Female ,Endothelium, Vascular ,business ,Dimerization - Abstract
Postmenopausal women have high incidence of cardiovascular events as estrogen deficiency can cause endothelial dysfunction. Vitamin D is reported to be beneficial on endothelial function, but it remains controversial whether vitamin D is effective for endothelial dysfunction under the treatment for osteoporosis in postmenopausal women. The aim of this study was to evaluate the endothelial protective effect of eldecalcitol (ELD) in ovariectomized (OVX) rats. ELD (20 ng/kg) was orally administrated five times a week for 4 weeks from 1 day after surgery. After that, flow-mediated dilation (FMD) as an indicator of endothelial function was measured by high-resolution ultrasound in the femoral artery of living rats. ELD ameliorated the reduction of FMD in OVX rats. ELD inhibited the increase in NOX4, nitrotyrosine, and p65 and the decrease in dimer/monomer ratio of nitric oxide synthase in OVX rat femoral arteries. ELD also prevented the decrease in peroxisome proliferator-activated receptor gamma (PPARγ) in femoral arteries and cultured endothelial cells. Although PPARγ is known to inhibit osteoblastogenesis, ELD understandably increased bone mineral density of OVX rats without increase in PPARγ in bone marrow. These results suggest that ELD prevented the deterioration of endothelial function under condition of preventing bone loss in OVX rats. This endothelial protective effect of ELD might be exerted through improvement of endothelial nitric oxide synthase uncoupling, which is mediated by an antioxidative effect through normalization of vascular PPARγ/NF-κB signaling.
- Published
- 2015
41. Stable isotope labeling approaches for NMR characterization of glycoproteins using eukaryotic expression systems
- Author
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Koichi Kato, Rina Yogo, Hirokazu Yagi, Maho Yagi-Utsumi, and Saeko Yanaka
- Subjects
0301 basic medicine ,Glycan ,Protein Engineering ,Biochemistry ,law.invention ,03 medical and health sciences ,law ,Molecule ,Nuclear Magnetic Resonance, Biomolecular ,Spectroscopy ,Glycoproteins ,chemistry.chemical_classification ,Carbon Isotopes ,Molecular mass ,biology ,Nuclear magnetic resonance spectroscopy ,Oligosaccharide ,Yeast ,Recombinant Proteins ,Immunoglobulin Fc Fragments ,030104 developmental biology ,Eukaryotic Cells ,chemistry ,Isotope Labeling ,biology.protein ,Recombinant DNA ,Glycoprotein - Abstract
Glycoproteins are characterized by the heterogeneous and dynamic nature of their glycan moieties, which hamper crystallographic analysis. NMR spectroscopy provides potential advantages in dealing with such complicated systems, given that the target molecules can be isotopically labeled. Methods of metabolic isotope labeling in recombinant glycoproteins have been developed recently using a variety of eukaryotic production vehicles, including mammalian, yeast, insect, and plant cells, each of which has a distinct N-glycan diversification pathway. Yeast genetic engineering has enabled the overexpression of homogeneous high-mannose-type oligosaccharides with 13C labeling for NMR characterization of their conformational dynamics. The utility of stable isotope-assisted NMR spectroscopy has also been demonstrated using the Fc fragment of immunoglobulin G (IgG) as a model glycoprotein, providing useful information regarding intramolecular carbohydrate–protein interactions. Transverse relaxation optimization of intact IgG with a molecular mass of 150 kDa has been achieved by tailored deuteration of selected amino acid residues using a mammalian expression system. This offers a useful probe for the characterization of molecular interaction networks in multimolecular crowded systems typified by serum. Perspectives regarding the development of techniques for tailoring glycoform designs and isotope labeling of recombinant glycoproteins are also discussed.
- Published
- 2017
42. Induction of thioredoxin-1 in response to oxidative stress in dogs
- Author
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Yoshiyuki Matsuo, Yasushi Hara, Hai Tian, Shuntaro Munakata, Takuya Yogo, Junji Yodoi, Yasuji Harada, Yoshinori Nezu, Masahiro Tagawa, and Yoshikazu Tanaka
- Subjects
Pathology ,medicine.medical_specialty ,animal structures ,Urine ,Anesthesia, General ,medicine.disease_cause ,Andrology ,chemistry.chemical_compound ,Dogs ,Thioredoxins ,Antigen ,Blood plasma ,Animals ,Medicine ,Creatinine ,Isoflurane ,General Veterinary ,biology ,business.industry ,Deoxyguanosine ,General Medicine ,Oxygen ,Oxidative Stress ,chemistry ,8-Hydroxy-2'-Deoxyguanosine ,biology.protein ,Female ,Antibody ,Thioredoxin ,business ,Biomarkers ,Oxidative stress ,medicine.drug - Abstract
OBJECTIVE To determine whether thioredoxin (TRX)-1 can be used as a valid biomarker for oxidative stress in dogs. ANIMALS AND SAMPLES 10 Beagles and Madin-Darby canine kidney cells. PROCEDURES Madin-Darby canine kidney cells were used to verify antigen cross-reactivity between human and canine anti–TRX-antibodies. Dogs were assigned to receive 21% or 100% O2 (5 dogs/group) via an artificial respirator during a 3-hour period of isoflurane anesthesia (starting at 0 hours). Blood and urine samples were collected before (baseline) and at 6, 12, 24, and 48 hours after commencement of inhalation anesthesia. Concentrations of TRX-1 and 8-hydroxy-2′-deoxyguanosine (8-OHdG) in plasma and urine samples were analyzed; urine concentrations were reported as ratios against urine creatinine concentration. RESULTS Canine TRX-1 was recognized by monoclonal human anti-TRX-1 antibodies (clones of adult T-cell leukemia-derived factor [ADF]-11 and ADF21) by western blot analysis. Results of an ELISA indicated that plasma TRX-1 concentration and urine TRX-1-to-creatinine concentration ratio increased rapidly after the 3-hour period of hyperoxia with maximal peaks at 12 and 6 hours, respectively. Urine 8-OHdG-to-creatinine concentration ratio also increased significantly after hyperoxia induction. However, unlike the rapid increase in urine TRX-1-to-creatinine concentration ratio, maximal urine 8-OHdG-to-creatinine concentration ratio was attained at 48 hours after hyperoxia induction. These variables remained unchanged from baseline in the control group. CONCLUSIONS AND CLINICAL RELEVANCE Results indicated that human anti-TRX monoclonal antibodies cross-reacted with canine TRX, and plasma TRX-1 concentrations were rapidly increased in dogs following an oxidative stress challenge. Thus, TRX may be a valuable clinical biomarker for detecting oxidative stress more rapidly than 8-OHdG in dogs.
- Published
- 2015
43. Nicorandil prevents sirolimus-induced production of reactive oxygen species, endothelial dysfunction, and thrombus formation
- Author
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Nobuhiko Ishizuka, Kenji Yogo, Naoto Fukuyama, Kenichi Serizawa, Ken Aizawa, Naoko Higashijima, Koichi Endo, Hideyuki Ishida, Katsuya Hirano, and Youko Takahari
- Subjects
Male ,Cell Survival ,Endothelial cells ,Pharmacology ,Superoxide dismutase ,Restenosis ,Testis ,medicine ,Animals ,Humans ,Thrombus ,Endothelial dysfunction ,Nicorandil ,Cells, Cultured ,chemistry.chemical_classification ,Sirolimus ,Mice, Inbred ICR ,Reactive oxygen species ,NADPH oxidase ,biology ,Superoxide Dismutase ,business.industry ,lcsh:RM1-950 ,NADPH Oxidases ,Thrombosis ,Arteries ,medicine.disease ,Coronary Vessels ,Up-Regulation ,Disease Models, Animal ,lcsh:Therapeutics. Pharmacology ,chemistry ,Anesthesia ,biology.protein ,Molecular Medicine ,business ,Anti-Arrhythmia Agents ,medicine.drug - Abstract
Sirolimus (SRL) is widely used to prevent restenosis after percutaneous coronary intervention. However, its beneficial effect is hampered by complications of thrombosis. Several studies imply that reactive oxygen species (ROS) play a critical role in endothelial dysfunction and thrombus formation. The present study investigated the protective effect of nicorandil (NIC), an anti-angina agent, on SRL-associated thrombosis. In human coronary artery endothelial cells (HCAECs), SRL stimulated ROS production, which was prevented by co-treatment with NIC. The preventive effect of NIC on ROS was abolished by 5-hydroxydecanoate but not by 1H-[1,2,4]oxadiazolo[4,3-a]quinoxalin-1-one. NIC also inhibited SRL-induced up-regulation of NADPH oxidase subunit p22(phox) mRNA. Co-treatment with NIC and SRL significantly up-regulated superoxide dismutase 2. NIC treatment significantly improved SRL-induced decrease in viability of HCAECs. The functional relevance of the preventive effects of NIC on SRL-induced ROS production and impairment of endothelial viability was investigated in a mouse model of thrombosis. Pretreatment with NIC inhibited the SRL-induced acceleration of FeCl3-initiated thrombus formation and ROS production in the testicular arteries of mice. In conclusion, NIC prevented SRL-induced thrombus formation, presumably due to the reduction of ROS and to endothelial protection. The therapeutic efficacy of NIC could represent an additional option in the prevention of SRL-related thrombosis.
- Published
- 2015
44. Growth inhibitory effects of miR‐221 and miR‐222 in non‐small cell lung cancer cells
- Author
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Yoshinori Hasegawa, Eiichi Maruyama, Naoyuki Yogo, Tetsunari Hase, Tomohiko Kakumu, Mitsuo Sato, Ryo Yamashita, Masashi Kondo, and Yoshitaka Sekido
- Subjects
Cancer Research ,Epithelial-Mesenchymal Transition ,Lung Neoplasms ,Cell division ,Population ,Apoptosis ,Antineoplastic Agents ,Biology ,epithelial–mesenchymal transition ,Real-Time Polymerase Chain Reaction ,S Phase ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,microRNA ,medicine ,Humans ,Radiology, Nuclear Medicine and imaging ,Epithelial–mesenchymal transition ,Lung cancer ,education ,Cancer Biology ,Original Research ,education.field_of_study ,Cell cycle ,medicine.disease ,microRNAs ,Cell biology ,Oncology ,Cell culture ,cell cycle ,Cell Division - Abstract
Both pro- and anti-oncogenic roles of miR-221 and miR-222 microRNAs are reported in several types of human cancers. A previous study suggested their oncogenic role in invasiveness in lung cancer, albeit only one cell line (H460) was used. To further evaluate involvement of miR-221 and miR-222 in lung cancer, we investigated the effects of miR-221 and miR-222 overexpression on six lung cancer cell lines, including H460, as well as one immortalized normal human bronchial epithelial cell line, HBEC4. miR-221 and miR-222 induced epithelial-to-mesenchymal transition (EMT)-like changes in a minority of HBEC4 cells but, unexpectedly, both the microRNAs rather suppressed their invasiveness. Consistent with the prior report, miR-221 and miR-222 promoted growth in H460; however, miR-221 suppressed growth in four other cell lines with no effects in one, and miR-222 suppressed growth in three cell lines but promoted growth in two. These are the first results to show tumor-suppressive effects of miR-221 and miR-222 in lung cancer cells, and we focused on clarifying the mechanisms. Cell cycle and apoptosis analyses revealed that growth suppression by miR-221 and miR-222 occurred through intra-S-phase arrest and/or apoptosis. Finally, lung cancer cell lines transfected with miR-221 or miR-222 became more sensitive to the S-phase targeting drugs, possibly due to an increased S-phase population. In conclusion, our data are the first to show tumor-suppressive effects of miR-221 and miR-222 on lung cancer, warranting testing their potential as therapeutics for the disease.
- Published
- 2015
45. Small Fish Robot
- Author
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Yogo Takada
- Subjects
Fishery ,Robot ,%22">Fish ,Biology - Published
- 2015
46. The Performance of Jatropha Curcas Linn. Capsule Husk as Feedstocks Biogas in One Phase Anaerobic Digestion
- Author
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Mel Mairziwan, Anggi Nindita, Tony Liwang, Satriyo Krido Wahono, Praptiningsih Gamawati Adinurani, Andi Sasmito, S. Roy Hendroko, and Yogo Adhi Nugroho
- Subjects
biorefinery ,biology ,Hydraulic retention time ,Chemistry(all) ,capsule husk ,Biogas ,General Medicine ,Pulp and paper industry ,Biorefinery ,biology.organism_classification ,Husk ,Methane ,Anaerobic digestion ,chemistry.chemical_compound ,Jatropha curcas Linn ,Volume (thermodynamics) ,chemistry ,Chemical Engineering(all) ,Environmental science ,Jatropha curcas - Abstract
Jatropa curcas Linn. (JcL) capsule husk was not recommended as biogas feedstocks. However for biorefinery purpose, several technologies have been conducting to solve this problem. This research reported quantity and quality comparison of Dry Husk Jcl (DH-JcL) in one phase system of batch digester compare with semi continuous digester. HDPE drum of 80 L working volume used as digester with 40 days hydraulic retention time. Feeding of DH-Jcl and solvent water was mixed on concentration of 1: 8. Research conclusion showed that semi continuous digester was better than batch digester. Biogas quality showed that methane content can reach 66.61% to 83.15% and biogas quantity in semi continuous digester can reach 0.016 m3 · kg–1 DH JcL. The result was not in optimize condition yet because ratio number of volatile fatty acids/ alkalinity showed 0.5, it was indicated unstable anaerobic degradation process of DH-JcL.
- Published
- 2015
- Full Text
- View/download PDF
47. The Study of Slurry Recirculation to Increase Biogas Productivity from Jatropha curcas Linn. Capsule Husk in Two Phase Digestion
- Author
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Tony Liwang, Praptiningsih Gamawati Adinurani, Maizirwan Mel, Yogo Adhi Nugroho, Agus Setyo Yudhanto, Anggi Nindita, S. Roy Hendroko, and Andi Sasmito
- Subjects
two-phase digester system ,Waste management ,biology ,Methanogenesis ,Chemistry ,Biogas ,biology.organism_classification ,Husk ,Hydrolysis ,Digestion (alchemy) ,dried husk Jatropha curcas Linn ,Energy(all) ,Biofilter ,Slurry ,Jatropha curcas ,slurry recirculation - Abstract
The purpose of this study was to obtain the best slurry recirculation treatment for two-phase digester system which produced the highest biogas production in the DH-JcL substrate. The study was conducted in the laboratory using 750 mL and 2 L of plastic digester and biofilter plastic as growth immobilize. The slurry recirculation treatment consisted of the recirculation of 50% slurry to methanogenesis digester, 50% slurry to hydrolysis digester, and 25% slurry to hydrolysis digester combined with 25% to methanogenesis digester. The highest daily biogas production was obtained from the recirculation of 50% slurry to hydrolysis digester. However, the best slurry recirculation treatment, which was more stable in longer period, was 50% slurry to methanogenesis digester.
- Published
- 2015
48. Biological Purification System: Integrated Biogas from Small Anaerobic Digestion and Natural Microalgae
- Author
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Roy Hendroko Setyobudi, Yogo Adhi Nugroho, Andi Sasmito, Satriyo Krido Wahono, Tony Liwang, Praptiningsih Gamawati Adinurani, Anggi Nindita, and Salafudin
- Subjects
Clarias gariepinus ,Biological purification ,biology ,Chemistry(all) ,Chemistry ,bio-refinery ,microalgae ,bio-methane ,General Medicine ,Pulp and paper industry ,Photosynthesis ,biology.organism_classification ,Husk ,Anaerobic digestion ,chemistry.chemical_compound ,Pilot plant ,Biogas ,CO2 levels ,Chlorophyll ,Carbon dioxide ,Chemical Engineering(all) - Abstract
Photosynthetic pigments, including chlorophyll, have an important role since they provide the oxygen and the source of energy for all living things. Plant and algae growth is affected by the photosynthesis speed which depends on the availability of carbon dioxide (CO 2 ). This paper reports on the pilot plant scale study of the impact of 20% to 50% CO 2 on biogas into the growing medium of microalgae which obtained bio-methane purification results as gaseous bio-fuels. Research material was produced from the Jatropha curcas Linn. husk biogas digester and a 0.15 m 3 HDPE drum was used as a purification. The purification tank was filled with Catfish ( Clarias gariepinus ) farm water which grew “wild” microalgae naturally. The water was fed from the top with continuous flow of (16 to 31) L· min –1 and the biogas was fed from the bottom at (18 to 29) L · min –1 . CO 2 level data of biogas was measured by orsat apparatus and processed with t test. The results achieved average efficiency reduction levels of CO 2 on 50% in two cycles (24% in the first and 26% in the second).
- Published
- 2015
- Full Text
- View/download PDF
49. Construction for Δ-12 Fatty Acid Desaturase (FAD2) Silencing to Improve Oil Quality of Jatropha curcas Linn
- Author
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Tony Liwang, Yogo Adhi Nugroho, Roy Hendroko Setyobudi, Condro Utomo, Andrea Putri Subroto, and Chris Darmawan
- Subjects
inverted repeat ,biology ,Linoleic acid ,intron-spliced inverted repeat ,Δ 12 fatty acid desaturase ,biology.organism_classification ,Palmitic acid ,gene silencing ,chemistry.chemical_compound ,Transformation (genetics) ,Oleic acid ,Fatty acid desaturase ,Jatropha curcas Linn ,chemistry ,Biochemistry ,Energy(all) ,biology.protein ,Gene silencing ,Food science ,Stearic acid ,Jatropha curcas - Abstract
The predominant fatty acids of jatropha oil are oleic acid (42.02%), linoleic acid (35.38%), palmitic acid (14.54%), and stearic acid (6.3%). Ratio of fatty acids content of jatropha oil, especially for oleic acid (42.02%) compare to linoleic acid (35.38%) is approximately equal. The oxidation stability of jatropha oil showed that methyl oleate is around 2.79 h and methyl linoleate is 0.94 h. Therefore, the presence of methyl linoleate will decrease the value oxidation stability of jatropha oil. The objective of the research was to modify fatty acid composition, especially to increase oleic acid and to reduce linoleic acid content of jatropha oil. To achieve the objective construction of transformation vectors for Δ 12 fatty acid desaturase FAD2 gene silencing was designed. In two transformation vectors, FAD2 gene fragment was constructed in inverted repeat (IR) and intron-spliced inverted repeat (ISIR) orientations to generate hair-pin RNAs (hpRNA).
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- 2015
- Full Text
- View/download PDF
50. Vitamin D Receptor Signaling Enhances Locomotive Ability in Mice
- Author
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Keisuke Tanaka, Yoshihito Tashiro, Koichi Endo, Satoshi Takeda, Ken Aizawa, Kenji Yogo, Sadaoki Sakai, Miho Suzuki, and Michinori Hirata
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medicine.medical_specialty ,biology ,business.industry ,Endocrinology, Diabetes and Metabolism ,Osteoporosis ,Anatomy ,Eldecalcitol ,medicine.disease ,Calcitriol receptor ,Neuromuscular junction ,Myelin basic protein ,Extensor digitorum longus muscle ,chemistry.chemical_compound ,medicine.anatomical_structure ,Endocrinology ,chemistry ,Internal medicine ,Sarcopenia ,medicine ,biology.protein ,Orthopedics and Sports Medicine ,business ,Acetylcholine receptor - Abstract
Bone fractures markedly reduce quality of life and life expectancy in elderly people. Although osteoporosis increases bone fragility, fractures frequently occur in patients with normal bone mineral density. Because most fractures occur on falling, preventing falls is another focus for reducing bone fractures. In this study, we investigated the role of vitamin D receptor (VDR) signaling in locomotive ability. In the rotarod test, physical exercise enhanced locomotive ability of wild-type (WT) mice by 1.6-fold, whereas exercise did not enhance locomotive ability of VDR knockout (KO) mice. Compared with WT mice, VDR KO mice had smaller peripheral nerve axonal diameter and disordered AChR morphology on the extensor digitorum longus muscle. Eldecalcitol (ED-71, ELD), an analog of 1,25(OH)2 D3 , administered to rotarod-trained C57BL/6 mice enhanced locomotor performance compared with vehicle-treated nontrained mice. The area of AChR cluster on the extensor digitorum longus was greater in ELD-treated mice than in vehicle-treated mice. ELD and 1,25(OH)2 D3 enhanced expression of IGF-1, myelin basic protein, and VDR in rat primary Schwann cells. VDR signaling regulates neuromuscular maintenance and enhances locomotive ability after physical exercise. Further investigation is required, but Schwann cells and the neuromuscular junction are targets of vitamin D3 signaling in locomotive ability.
- Published
- 2014
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