1. Deltex3 inhibits Epithelial Mesenchymal Transition in Papillary Thyroid Carcinoma via promoting ubiquitination of XRCC5 to regulate the AKT signal pathway
- Author
-
Zhen Liu, Yonglian Huang, Ailian Wang, Jingni He, Lidong Wang, Chenxi Liu, Xiaodan Sun, Zhennan Ma, and Mingyue Guo
- Subjects
Oxidoreductase complex ,Gene knockdown ,medicine.diagnostic_test ,biology ,endocrine system diseases ,Chemistry ,epithelial-mesenchymal transition ,Vimentin ,AKT signal pathway ,Flow cytometry ,Small hairpin RNA ,Oncology ,Western blot ,Deltex3 ,Cancer research ,medicine ,biology.protein ,papillary thyroid carcinoma ,Epithelial–mesenchymal transition ,Protein kinase B ,XRCC5 ,Research Paper - Abstract
Background: Papillary thyroid carcinoma (PTC) is one of the most common endocrine malignant tumors. Poor prognoses such as high recurrence rate always appear in PTC patients with cervical lymph node metastasis. The process of ubiquitination plays important roles in PTC. As ubiquitin E3 ligases, Deltex (DTX) family proteins were reported to associate with multiple cancers. However, functions and mechanisms of DTX3 in PTC are currently unknown. Methods: In this study, DTX3 expressions were examined in 114 PTC and paired paracancerous normal tissues through quantitative real-time polymerase chain reaction and western blot. The clinical significances of DTX3 expressions in PTC patients were also investigated. After stable transfection with either short hairpin RNA to knock down DTX3 expression or full-length complementary DNA to upregulate DTX3 expression, changes of malignant phenotypes in two PTC cell lines K1 and TPC-1 were observed using cell viability, flow cytometry, wound healing and transwell assays. Afterwards, altered expressions of epithelial-mesenchymal transition (EMT) and AKT signal pathway related proteins were measured by western blot. Immunoprecipitation and mass spectrometry (IP-MS), immunofluorescence and Co-IP were utilized to identify the possible DTX3 interacting proteins. Results: Both mRNA and protein expressions of DTX3 were lower in PTC tissues and correlated with the presence of cervical lymph node metastasis (P
- Published
- 2021