1. Molecular cloning, tissue distribution and the expression of cystine/glutamate exchanger (xCT, SLC7A11) in different tissues during development in broiler chickens
- Author
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Chengbo Yang, Liju Ni, B Schindell, W Li, Martin Nyachoti, Janghan Choi, Shangxi Liu, Xiaoya Zhao, and Joshua Gong
- Subjects
Cystine ,Development ,SLC7A11 ,03 medical and health sciences ,chemistry.chemical_compound ,Food Animals ,Complementary DNA ,Gene expression ,lcsh:SF1-1100 ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Messenger RNA ,biology ,0402 animal and dairy science ,04 agricultural and veterinary sciences ,Glutathione ,Cystine/glutamate transporter ,040201 dairy & animal science ,Molecular biology ,Amino acid ,Broiler chickens ,chemistry ,biology.protein ,Molecular Nutrition ,Animal Science and Zoology ,lcsh:Animal culture ,Cysteine - Abstract
The cystine/glutamate exchanger (xCT, SLC7A11) is a component of the system Xc amino-acid antiporter that is able to export glutamate and import cysteine into cells. The xCT amino acid exchanger has received a lot of attention, due to the fact that cysteine is an essential substrate for the synthesis of glutathione (GSH), an endogenous antioxidant in cells. The objective of this research was to clone the full-length cDNA of chicken xCT, and to investigate the gene expression of xCT in different tissues, including intestinal segments of broiler chickens during development. The full-length cDNA of chicken xCT (2,703 bp) was obtained from the jejunum by reverse transcription-PCR and sequenced. Homology tests showed that chicken xCT had 80.4%, 80.2%, and 71.2% homology at the nucleotide level with humans, cattle, and rats, respectively. Likewise, amino acid sequence analysis showed that chicken xCT protein is 86.4%, 79.3%, and 75.6% homologous with humans, cattle, and rats, respectively. Additionally, phylogenetic analysis indicated that chicken xCT genes share a closer genetic relationship with humans and cattle, than with rats. The chicken xCT protein has 12 transmembrane helixes, 6 extracellular loops, and 5 intracellular loops. The mRNA of xCT was detected in all tissues, including intestinal segments, in which the mRNA expression of xCT was significantly higher (P
- Published
- 2020