1. Chromosomal assembly of the Antarctic toothfish (Dissostichus mawsoni) genome using third-generation DNA sequencing and Hi-C technology
- Author
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Euna Jo, E. Choi, Seok-Gwan Choi, Sangdeok Chung, Jeong-Hoon Kim, Hyun Park, Jinmu Kim, Hyun-Woo Kim, and Seung Jae Lee
- Subjects
0303 health sciences ,Dissostichus ,Ecology ,biology ,Gene prediction ,030302 biochemistry & molecular biology ,biology.organism_classification ,Genome ,DNA sequencing ,03 medical and health sciences ,Evolutionary biology ,Nototheniidae ,Animal Science and Zoology ,Antarctic toothfish ,Gene ,Ecology, Evolution, Behavior and Systematics ,030304 developmental biology ,Reference genome - Abstract
The Antarctic toothfish, Dissostichus mawsoni, belongs to the Nototheniidae family and is distributed in sub-zero temperatures below S60° latitude in the Southern Ocean. Therefore, it is an attractive model species to study the stenothermal cold-adapted character state. In this study, we successfully generated highly contiguous genome sequences of D. mawsoni, which contained 1 062 scaffolds with a N50 length of 36.98 Mb and longest scaffold length of 46.82 Mb. Repetitive elements accounted for 40.87% of the genome. We also inferred 32 914 protein-coding genes using in silico gene prediction and transcriptome sequencing and detected splicing variants using Isoform-Sequencing (Iso-Seq), which will be invaluable resource for further exploration of the adaptation mechanisms of Antarctic toothfish. This new high-quality reference genome of D. mawsoni provides a fundamental resource for a deeper understanding of cold adaptation and conservation of species.
- Published
- 2021