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Biodiesel is one of the renewable energy sources derived from living organisms and is an alternative to petroleum fuels that cause environmental pollution. Biodiesel is specified as the fatty acid alkyl esters (FAAE) formed as a result of the transesterification reactions of various alcohols and triglycerides with catalysts. The catalyst plays an important role in biodiesel production. These catalysts can be synthesized from chemical or natural substances as homogeneous or heterogeneous. Some natural waste materials such as egg shell and fish scales have started to be preferred in catalyst synthesis due to their low cost, accessibility and support for waste recycling and minimizing. In this study, a high-performance heterogeneous catalyst was synthesized in order to reduce cost, increase the reaction rate and evaluate waste fish scales in biodiesel production. Waste fish scales were characterized by Thermogravimetric Analysis (TGA). The novel catalyst was prepared from waste Seabass (Dicentrarchus Labrax) fish scales by calcination at different temperatures (800, 900, 950 and 1000 °C). The best calcination temperature was determined by using as X-Ray Diffraction (XRD) techniques as 1000 °C and the waste fish scale catalyst was shortened as WFSC. The novel WFSC was characterized by BET specific surface area, Scanning Electron Microscopy (SEM) and Fourier Transform Infrared Spectroscopy (FT-IR). Finally, the novel WFSC were used for biodiesel production. Biodiesel yield was calculated as 85 %. Additionally, this novel catalyst reduces chemical consumption in biodiesel production, with its reusability. Due to the obtained results, it can be qualified as an eco-friendly catalyst.