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Photoacoustic spectroscopy (PAS) has been widely used to analyze biological samples. For example, PAS has been applied to the study of blood which could be related to some diseases. In the present research, the optical absorption spectra of Fisher rat blood, with hepatic damage, were obtained by PAS. Also, the daily intake of turmeric (Curcuma longa), as a protection of liver damage, was evaluated through the characteristic optical absorption peaks of blood, α(585 nm), β(550 nm), and γ(420 nm), where it is possible to associate them with differences in hemoglobin concentrations of the studied samples, by means of the peak ratios γ/β and γ/α, for each analyzed group.

In recent years, has been reported an increasing interest to study vegetables and fruits in order to know their benefits to prevent different types of health problems. Curcuma longa has a great potential to prevent diseases as different types of cancer. In the present study, C. longa samples, of different trademarks, were optically and thermally characterized by using photoacoustic spectroscopy and photopyroelectric techniques, obtaining their optical absorption spectra, optical absorption length spectra, the thermal diffusivity, effusivity, conductivity and heat capacity per unit volume, observing optical absorptions corresponding to antioxidant elements, reported such as beneficial for health. These properties also could be important in the food industry for the commercialization of functional foods.

Photoacoustic spectroscopy (PAS) is singled out by its capability to resolve the optical absorption spectra of each element, of a multicomponent system, by the named phase resolved method. The possibility of the use of this method is due to the fact that the Photoacoustic signal is not only sensitive to the optical absorption of the sample as well as the non-radiative relaxation times of its elements. In the present study we compare and analyze, by phase resolved method, Fisher rat blood in four groups: healthy rat blood, liver cancer rat blood, healthy rat blood given by a daily dose of Curcuma longa, and liver cancer rat blood given by a daily dose of Curcuma longa (turmeric), where the optical absorption spectra of the analyzed blood samples was obtained by PAS, in a wavelength range from 350 nm to 650 nm. Three repetitions of 60 μl of blood for each group was done. By using the phase resolved method a new optical absorption band suggestive of methemoglobin was found, observing the benefits of the intake of turmeric due to the fact that the optical absorption spectrum of the Cancer + turmeric group diminish 14% with respect to the Cancer group, anticipating a lower concentration of methemoglobin in blood.