1. Rapid detection of biofilm‐producing Candida species via MALDI‐TOF mass spectrometry
- Author
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J.P. Pimenta, Paula Augusta Dias Fogaça de Aguiar, Nagela Bernadelli Sousa Silva, Ralciane de Paula Menezes, Denise Von Dolinger de Brito Röder, Mário Paulo Amante Penatti, and Tomaz de Aquino Moreira more...
- Subjects
Candida parapsilosis ,Applied Microbiology and Biotechnology ,Microbiology ,Candida tropicalis ,03 medical and health sciences ,chemistry.chemical_compound ,Candida krusei ,Candida albicans ,Humans ,Candida ,030304 developmental biology ,0303 health sciences ,biology ,Candida glabrata ,030306 microbiology ,Chemistry ,Biofilm ,Candidemia ,General Medicine ,biochemical phenomena, metabolism, and nutrition ,biology.organism_classification ,Matrix-assisted laser desorption/ionization ,Biofilms ,Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization ,Formazan ,Biotechnology - Abstract
Aims The aim of this study was to evaluate the formation of biofilm by Candida spp. isolated from the bloodstream, using traditional spectrophotometric methodologies. In addition, the goal was to compare the results with those obtained through MALDI-TOF/MS, as well as to verify its use as a potential tool for the detection of biofilm-forming strains. Methods and results Hundred and thirteen isolates of Candida spp. were studied: 41 were Candida albicans, 27 C. tropicalis, 18 C. glabrata, 17 C. parapsilosis and 10 C. krusei. Metabolic activity was determined through the tetrazolium salt (XTT) reduction assay and biomass by staining with Crystal Violet. All isolates were able to form biofilm, 94% of which were strong producers, with high biomass quantification (95%; 107/113) and high metabolic activity (99%; 112/113). Mass spectra of the biofilm-producing isolates showed differences in the intensity of mass peaks when compared with the spectra of the nonproducing strains. Conclusions It was demonstrated that MALDI-TOF/MS was able to detect specific biofilm proteins, as the mass spectra of the isolates presented differences when compared with nonproducing strains. Significance and impact of the study MALDI-TOF/MS can become a valuable tool for biofilm detection at the moment of the identification of the microorganism, thus contributing greatly to the management of patients with Candidemia. more...
- Published
- 2021
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