1. Effective Production of Phenolic Compounds with Health Benefits in Pigeon Pea [Cajanus cajan (L.) Millsp.] Hairy Root Cultures
- Author
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Zi-Ying Wang, Xiao-Jie Xu, Yao Lu, Jiao Jiao, Qing-Yan Gai, Xin Wang, Jing Liu, and Yu-Jie Fu
- Subjects
0106 biological sciences ,biology ,Cajaninstilbene acid ,Chemistry ,010401 analytical chemistry ,Biomass ,General Chemistry ,Health benefits ,biology.organism_classification ,01 natural sciences ,0104 chemical sciences ,Human health ,Cajanus ,Nutraceutical ,Biological property ,Yield (chemistry) ,Food science ,General Agricultural and Biological Sciences ,010606 plant biology & botany - Abstract
Phenolic compounds in pigeon pea possess various biological properties beneficial to human health. In this study, pigeon pea hairy root cultures (PPHRCs) were developed as an effective in vitro platform for the production of phenolic compounds. A high-productive hairy root line was screened and characterized, and its culture conditions were optimized in terms of biomass productivity and phenolic yield. The comparative profiling of 10 phenolic compounds in PPHRCs and pigeon pea natural resources (seeds, leaves, and roots) was achieved by ultra-high-performance liquid chromatography-tandem mass spectrometry analysis. The total phenolic yield in PPHRCs (3278.44 μg/g) was much higher than those in seeds (68.86 μg/g) and roots (846.03 μg/g), and comparable to leaves (3379.49 μg/g). Notably, PPHRCs exhibited superiority in the yield of the most important health-promoting compound cajaninstilbene acid (2996.23 μg/g) against natural resources (4.42-2293.31 μg/g). Overall, PPHRCs could serve as promising potential alternative sources for the production of phenolic compounds with nutraceutical/medicinal values.
- Published
- 2020