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9 results on '"hyposoter didymator"'

1. Isolation and characterization of microsatellites in the endoparasitic ichneumonid wasp carrying a polydnavirus Hyposoter didymator

Author

Marie Frayssinet, Anne-Nathalie Volkoff, Denis Bourguet, Philippe Audiot, Véronique Jouan, Centre de Biologie pour la Gestion des Populations (UMR CBGP), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Institut National de la Recherche Agronomique (INRA)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), AIP Bioressources EcoMicro de l'Inra, Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)-Institut Agro - Montpellier SupAgro, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Centre de Coopération Internationale en Recherche Agronomique pour le Développement (Cirad)-Centre international d'études supérieures en sciences agronomiques (Montpellier SupAgro)-Université de Montpellier (UM)-Institut de Recherche pour le Développement (IRD [France-Sud])-Institut national d’études supérieures agronomiques de Montpellier (Montpellier SupAgro), and Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE)

Subjects
food.ingredient, lutte biologique, ravageur agricole, Helicoverpa armigera, Genome, Parasitoid, food, hyposoter didymator, Botany, Genetics, guêpe, Genotyping, [SDV.BDD]Life Sciences [q-bio]/Development Biology, Ecology, Evolution, Behavior and Systematics, biology, Polydnavirus, génome, [SDV.BA]Life Sciences [q-bio]/Animal biology, Pyrosequencing, insecta, biology.organism_classification, Ichneumonidae, parasite, Microsatellite, Ichnovirus, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, france
Abstract

The wasp Hyposoter didymator (Hymenoptera, Ichneumonidae) parasitizes several agricultural pest moths and could therefore be used in biological control. The 454 FLX Titanium pyrosequencing technology was used to define two distinct sets of multiplex combining 14 polymorphic microsatellite loci: 10 (referred to as HD) located within the genome of H. didymator and 4 (referred to as HdIV) located within the Ichnovirus genome which is integrated into the wasp genome. Genotyping of two populations collected in France on Helicoverpa armigera revealed that most of the loci are independent and at Hardy-Weinberg equilibrium.

Published
2014
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2. Extensive transcription analysis of the Hyposoter didymator ichnovirus genome in permissive and non-permissive lepidopteran host species

Author

Gabor Gyapay, Isabelle Darboux, François Cousserans, Anne-Nathalie Volkoff, Eric Wajnberg, Véronique Jouan, Patrícia Milano, Tristan Dorémus, Fernando Luis Cônsoli, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Escola Super Agr Luiz Queiroz, Dept Entomol & Acarol, Lab Interacoes Insetos, Universidade de São Paulo (USP), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Université Paris-Saclay-Institut de Biologie François JACOB (JACOB), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Universidade de São Paulo = University of São Paulo (USP), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), and Volkoff, Anne Nathalie

Subjects
[SDV.SA]Life Sciences [q-bio]/Agricultural sciences, Transcription, Genetic, Biodiversité et Ecologie, lcsh:Medicine, Genome, Parasitoid, hyposoter didymator, transcription d'adn, Gene Order, Cluster Analysis, lcsh:Science, Genetics, noctuelle, Multidisciplinary, biology, Agriculture, Agricultural sciences, Lepidoptera, Host-Pathogen Interactions, expression des gènes, Research Article, food.ingredient, Molecular Sequence Data, Genome, Viral, Spodoptera, Microbiology, Biodiversity and Ecology, food, Gene family, Animals, guêpe, génomique des populations, Gene, Molecular Biology, Gene Expression Profiling, lcsh:R, Organisms, Biology and Life Sciences, spodoptera frugiperda, biology.organism_classification, Gene expression profiling, Viral Tropism, Pseudoplusia, Polydnaviridae, Parasitology, lcsh:Q, Ichnovirus, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, Transcriptome, Sciences agricoles
Abstract

Ichnoviruses are large dsDNA viruses that belong to the Polydnaviridae family. They are specifically associated with endoparasitic wasps of the family Ichneumonidae and essential for host parasitization by these wasps. We sequenced the Hyposoter didymator Ichnovirus (HdIV) encapsidated genome for further analysis of the transcription pattern of the entire set of HdIV genes following the parasitization of four different lepidopteran host species. The HdIV genome was found to consist of at least 50 circular dsDNA molecules, carrying 135 genes, 98 of which formed 18 gene families. The HdIV genome had general features typical of Ichnovirus (IV) genomes and closely resembled that of the IV carried by Hyposoter fugitivus. Subsequent transcriptomic analysis with Illumina technology during the course of Spodoptera frugiperda parasitization led to the identification of a small subset of less than 30 genes with high RPKM values in permissive hosts, consisting with these genes encoding crucial virulence proteins. Comparisons of HdIV expression profiles between host species revealed differences in transcript levels for given HdIV genes between two permissive hosts, S. frugiperda and Pseudoplusia includens. However, we found no evident intrafamily gene-specific transcription pattern consistent with the presence of multigenic families within IV genomes reflecting an ability of the wasps concerned to exploit different host species. Interestingly, in two non-permissive hosts, Mamestra brassiccae and Anticarsia gemmatalis (most of the parasitoid eggs were eliminated by the host cellular immune response), HdIV genes were generally less strongly transcribed than in permissive hosts. This suggests that successful parasitism is dependent on the expression of given HdIV genes exceeding a particular threshold value. These results raise questions about the mecanisms involved in regulating IV gene expression according to the nature of the lepidopteran host species encountered.

Published
2014
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3. Venom gland extract is not required for successful parasitism in the polydnavirus-associated endoparasitoid Hyposoter didymator (Hym. Ichneumonidae) despite the presence of numerous novel and conserved venom proteins

Author

Eric Wajnberg, Tristan Dorémus, Jean-Luc Gatti, Serge Urbach, Véronique Jouan, Dominique Colinet, Anne-Nathalie Volkoff, Marylène Poirié, Carole Azéma-Dossat, Julie Poulain, François Cousserans, Edith Demettre, Isabelle Darboux, Marc Ravallec, Jean-Michel Escoubas, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), UMR 7254, Centre National de la Recherche Scientifique (CNRS), IG, Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Genoscope ('Parasitoid venoms' project), French National Research Agency (Paratoxose project) [ANR-09-BLAN-0243-01], Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut Sophia Agrobiotech (ISA), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA), ANR-09-BLAN-0243,PARATOXOSE(2009), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS)

Subjects
Male, 0106 biological sciences, Proteomics, Cellular immunity, Proteome, Hydrolases, [SDV]Life Sciences [q-bio], Wasps, Wasp Venoms, Venom, 01 natural sciences, Biochemistry, Polydnavirus, Ichneumonid, Immunity, Cellular, 0303 health sciences, Microscopy, biology, Spodoptera frugiperda, 3. Good health, Larva, Insect immunity, Insect Proteins, Female, Serine Proteinase Inhibitors, Molecular Sequence Data, Hyposoter didymator, Spodoptera, Serpin, 010603 evolutionary biology, complex mixtures, Host-Parasite Interactions, 03 medical and health sciences, Exocrine Glands, Botany, Animals, Amino Acid Sequence, Molecular Biology, 030304 developmental biology, Gene Expression Profiling, Sequence Analysis, DNA, Venom Protein, biology.organism_classification, Hymenoptera, Parasitoid, Insect Science, Campopleginae, Host regulation, Transcriptome, Parasitism success
Abstract

Corrigendum to “Venom gland extract is not required for successful parasitism in the polydnavirus-associated endoparasitoid Hyposoter didymator (Hym. Ichneumonidae) despite the presence of numerous novel and conserved venom proteins” [Insect Biochem. Mol. Biol. 43 (2013), 292–307] http://dx.doi.org/10.1016/j.ibmb.2013.07.001(UT:000329772300006); The venom gland is a conserved organ in Hymenoptera that shows adaptations associated with life-style diversification. Few studies have investigated venom components and function in the highly diverse parasitic wasps and all suggest that the venom regulates host physiology. We explored the venom of the endoparasitoid Hyposoter didymator (Campopleginae), a species with an associated polydnavirus produced in the ovarian tissue. We investigated the effects of the H. didymator venom on two physiological traits of the host Spodoptera frugiperda (Noctuidae): encapsulation response and growth rate. We found that H. didymator venom had no significant effect on host cellular immunity or development, suggesting that it does not contribute to parasitism success. The host physiology seemed to be modified essentially by the ovarian fluid containing the symbiotic polydnaviruses. Proteomic analyses indicated that the H. didymator venom gland produces a large variety of proteins, consistent with the classical hymenopteran venom protein signature, including: reprolysin-like, dipeptidyl peptidase IV, hyaluronidase, arginine kinase or allergen proteins. The venom extracts also contained novel proteins, encoded by venom genes conserved in Campopleginae ichneumonids, and proteins with similarities to active molecules identified in other parasitoid species, such as calreticulin, reprolysin, superoxide dismutase and serpin. However, some of these proteins appear to be produced only in small amounts or to not be secreted. Possibly, in Campopleginae carrying polydnaviruses, the host-modifying activities of venom became. redundant following the acquisition of polydnaviruses by the lineage.

Published
2013
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4. Hyposoter didymator uses a combination of passive and active strategies to escape from the Spodoptera frugiperda cellular immune response

Author

Serge Urbach, Eric Wajnberg, Anne-Nathalie Volkoff, Véronique Jouan, Marc Ravallec, Tristan Dorémus, Patrick Wincker, François Cousserans, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Institut de Génomique Fonctionnelle - Montpellier GenomiX (IGF MGX), Institut de Génomique Fonctionnelle (IGF), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS)-Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Université Montpellier 2 - Sciences et Techniques (UM2), Commissariat à l'énergie atomique et aux énergies alternatives (CEA), Institut Sophia Agrobiotech [Sophia Antipolis] (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (... - 2019) (UNS), Université Côte d'Azur (UCA)-Université Côte d'Azur (UCA)-Centre National de la Recherche Scientifique (CNRS), Genoscope ('Polydnavirus' projet), ANR-09-BLAN-0243-01, Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2)-Université de Montpellier (UM), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS)-Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Institut Sophia Agrobiotech (ISA), Institut National de la Recherche Agronomique (INRA)-Université Nice Sophia Antipolis (1965 - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS), ANR-09-BLAN-0243,PARATOXOSE(2009), Centre National de la Recherche Scientifique (CNRS)-Université Nice Sophia Antipolis (... - 2019) (UNS), and COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, Proteome, oviduct, Physiology, système immunitaire, ichnovirus, Wasps, mass spectrum analysis, Egg protein, venom, parasitisme des oeufs, apolipoprotéine, Polymerase Chain Reaction, 01 natural sciences, follicule ovarien, ichneumonidae, Tandem Mass Spectrometry, spectrométrie de masse, hyposoter didymator, symbiote, fluid, Immunity, Cellular, 0303 health sciences, Polydnavirus, fluide, mode de vie, Cell biology, symbiont, protéine, Larva, Insect Proteins, Female, polydnavirus, hymenoptera, protéine de synthèse, Braconidae, food.ingredient, particule virale, apolipoprotein, venin, Spodoptera, Biology, sepals, Calyx, braconidae, évasion immune, Viral Proteins, 03 medical and health sciences, life styles, Immune system, food, calice, Animals, oviducte, Ovum, 030304 developmental biology, Egg Proteins, Virion, protéines de surface, biology.organism_classification, Virology, 010602 entomology, ovarian follicle, Polydnaviridae, Insect Science, encapsulation, réponse de l'hôte, Ichnovirus, aphidiidae, endoparasite, [SDE.BE]Environmental Sciences/Biodiversity and Ecology, protein
Abstract

An endoparasitic life style is widespread among Hymenoptera, and various different strategies allowing parasitoids to escape from the host encapsulation response have been reported. Species carrying polydnaviruses (PDVs), such as the ichneumonid Hyposoter didymator, generally rely on the viral symbionts to evade host immune responses. In this work, we show that H. didymator eggs can evade encapsulation by the host in the absence of calyx fluid (containing the viral particles), whereas protection of the larvae requires the presence of calyx fluid. This evasion by the eggs depends on proteins associated with the exochorion. This type of local passive strategy has been described for a few species carrying PDVs. Immune evasion by braconid eggs appears to be related to PDVs or proteins synthesized in the oviducts being associated with the egg. We report that in H. didymator, by contrast, proteins already present in the ovarian follicles are responsible for the eggs avoiding encapsulation. Mass spectrometry analysis of the egg surface proteins revealed the presence of host immune-related proteins, including one with similarities with apolipophorin-III, and also the presence of three viral proteins encoded by IVSPERs (Ichnovirus Structural Protein Encoding Regions).

Published
2013
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5. Differential performance of a specialist and two generalist herbivores and their parasitoids on Plantago lanceolata

Author

Joanneke H. Reudler, Saskya van Nouhuys, Arjen Biere, Jeffrey A. Harvey, Multitrophic Interactions (MTI), and Terrestrial Ecology (TE)

Subjects
0106 biological sciences, Chrysodeixis chalcites, Hyposoter didymator, Iridoid Glucosides, Spodoptera, Generalist and specialist species, 010603 evolutionary biology, 01 natural sciences, Melitaea cinxia, Biochemistry, Article, Pheromones, Parasitoid, Host-Parasite Interactions, Multitrophic interactions, Spodoptera exigua, Botany, Plant defense against herbivory, Cotesia marginiventris, Animals, Cotesia melitaearum, Plantago, Ecology, Evolution, Behavior and Systematics, 2. Zero hunger, Immunocompromised host, Herbivore, biology, Host (biology), fungi, Iridoid glycosides, food and beverages, General Medicine, Feeding Behavior, biology.organism_classification, Adaptation, Physiological, Hymenoptera, Plant Leaves, 010602 entomology, Melitaea, Larva, Chemical defense, Plants, Edible, Butterflies
Abstract

The ability to cope with plant defense chemicals differs between specialist and generalist species. In this study, we examined the effects of the concentration of the two main iridoid glycosides (IGs) in Plantago lanceolata, aucubin and catalpol, on the performance of a specialist and two generalist herbivores and their respective endoparasitoids. Development of the specialist herbivore Melitaea cinxia was unaffected by the total leaf IG concentration in its host plant. By contrast, the generalist herbivores Spodoptera exigua and Chrysodeixis chalcites showed delayed larval and pupal development on plant genotypes with high leaf IG concentrations, respectively. This result is in line with the idea that specialist herbivores are better adapted to allelochemicals in host plants on which they are specialized. Melitaea cinxia experienced less post-diapause larval and pupal mortality on its local Finnish P. lanceolata than on Dutch genotypes. This could not be explained by differences in IG profiles, suggesting that M. cinxia has adapted in response to attributes of its local host plants other than to IG chemistry. Development of the specialist parasitoid Cotesia melitaearum was unaffected by IG variation in the diet of its host M. cinxia, a response that was concordant with that of its host. By contrast, the development time responses of the generalist parasitoids Hyposoter didymator and Cotesia marginiventris differed from those of their generalist hosts, S. exigua and C. chalcites. While their hosts developed slowly on high-IG genotypes, development time of H. didymator was unaffected. Cotesia marginiventris actually developed faster on hosts fed high-IG genotypes, although they then had short adult longevity. The faster development of C. marginiventris on hosts that ate high-IG genotypes is in line with the “immunocompromized host” hypothesis, emphasizing the potential negative effects of toxic allelochemicals on the host’s immune response.

Published
2011

6. Analysis of virion structural components reveals vestiges of the ancestral ichnovirus genome

Author

Fabrice Legeai, Gabor Gyapay, Fasséli Coulibaly, Patrick Wincker, Anne-Nathalie Volkoff, Catherine Béliveau, Jean-Michel Drezen, Sylvie Samain, Max Bergoin, Véronique Jouan, François Cousserans, Serge Urbach, Bertille Provost, Michel Cusson, Edith Demettre, Biologie Intégrative et Virologie des Insectes [Univ. de Montpellier II] (BIVI), Institut National de la Recherche Agronomique (INRA)-Université Montpellier 2 - Sciences et Techniques (UM2), Institut de Génomique Fonctionnelle (IGF), Université Montpellier 1 (UM1)-Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Genoscope - Centre national de séquençage [Evry] (GENOSCOPE), Université Paris-Saclay-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA), BioCampus Montpellier (BCM), Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Centre National de la Recherche Scientifique (CNRS), Structural Virology Group, Monash University [Clayton], Biologie des organismes et des populations appliquées à la protection des plantes (BIO3P), Institut National de la Recherche Agronomique (INRA)-Université de Rennes (UR)-AGROCAMPUS OUEST, Biological systems and models, bioinformatics and sequences (SYMBIOSE), Institut de Recherche en Informatique et Systèmes Aléatoires (IRISA), Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes (UR)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Inria Rennes – Bretagne Atlantique, Institut National de Recherche en Informatique et en Automatique (Inria), Laurentian Forestry Centre, Natural Resources Canada (NRCan), Institut de recherche sur la biologie de l'insecte UMR7261 (IRBI), Université de Tours (UT)-Centre National de la Recherche Scientifique (CNRS), ANR-05-BLAN-0199,EVPARASITOID,Physiologie, diversité et évolution des interactions hôte-parasitoïde(2005), ANR-09-BLAN-0243,PARATOXOSE(2009), Université de Montpellier (UM)-Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université Montpellier 2 - Sciences et Techniques (UM2)-Centre National de la Recherche Scientifique (CNRS), Institut National de la Recherche Agronomique (INRA)-Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-AGROCAMPUS OUEST, Université de Tours-Centre National de la Recherche Scientifique (CNRS), ANR-09-BLAN-0243,PARATOXOSE,Parasitoid Toxins : Origin, Specificity, Evolution(2009), Institut de Génomique d'Evry (IG), Institut de Biologie François JACOB (JACOB), Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Direction de Recherche Fondamentale (CEA) (DRF (CEA)), Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Commissariat à l'énergie atomique et aux énergies alternatives (CEA)-Université Paris-Saclay, Université Montpellier 1 (UM1)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Montpellier (UM)-Centre National de la Recherche Scientifique (CNRS), Virologie moléculaire et structurale (VMS), Institut National de la Recherche Agronomique (INRA)-Centre National de la Recherche Scientifique (CNRS), Université de Rennes 1 (UR1), Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées - Rennes (INSA Rennes), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Université de Rennes 1 (UR1), Institut National des Sciences Appliquées (INSA)-Université de Rennes (UNIV-RENNES)-Institut National des Sciences Appliquées (INSA)-Institut National de Recherche en Informatique et en Automatique (Inria)-Centre National de la Recherche Scientifique (CNRS)-Inria Rennes – Bretagne Atlantique, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro), Legeai, Fabrice, Programme non thématique - Appel à projets de recherche - Physiologie, diversité et évolution des interactions hôte-parasitoïde - - EVPARASITOID2005 - ANR-05-BLAN-0199 - BLANC - VALID, Blanc - - PARATOXOSE2009 - ANR-09-BLAN-0243 - Blanc - VALID, AGROCAMPUS OUEST, Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Institut national d'enseignement supérieur pour l'agriculture, l'alimentation et l'environnement (Institut Agro)-Université de Rennes 1 (UR1), and Université de Rennes (UNIV-RENNES)-Université de Rennes (UNIV-RENNES)-Institut National de la Recherche Agronomique (INRA)

Subjects
0106 biological sciences, Genome, Insect, Wasps, génomique fonctionnelle, 01 natural sciences, Genome, Nudivirus, chemistry.chemical_compound, Proviruses, hyposoter didymator, Convergent evolution, lcsh:QH301-705.5, [INFO.INFO-BI] Computer Science [cs]/Bioinformatics [q-bio.QM], Genetics, 0303 health sciences, [SDV.BIBS] Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], noctuelle, biology, Virulence, Polydnavirus, [SDV.BIBS]Life Sciences [q-bio]/Quantitative Methods [q-bio.QM], Virology/Virus Evolution and Symbiosis, 3. Good health, Multigene Family, ENDOPARASITOIDE LARVAIRE, Female, polydnavirus, Bracovirus, Research Article, lcsh:Immunologic diseases. Allergy, food.ingredient, Immunology, virus, Genome, Viral, macromolecular substances, 010603 evolutionary biology, Microbiology, Evolution, Molecular, 03 medical and health sciences, Viral Proteins, food, Virology, Animals, [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology, Molecular Biology, Gene, 030304 developmental biology, RELATION HOTE-PARASITE, STRUCTURE DU GENOME, VIRUS SYMBIOTIQUE, Ovary, fungi, Virion, biology.organism_classification, chemistry, lcsh:Biology (General), Polydnaviridae, Parasitology, Ichnovirus, [INFO.INFO-BI]Computer Science [cs]/Bioinformatics [q-bio.QM], lcsh:RC581-607, DNA
Abstract

Many thousands of endoparasitic wasp species are known to inject polydnavirus (PDV) particles into their caterpillar host during oviposition, causing immune and developmental dysfunctions that benefit the wasp larva. PDVs associated with braconid and ichneumonid wasps, bracoviruses and ichnoviruses respectively, both deliver multiple circular dsDNA molecules to the caterpillar. These molecules contain virulence genes but lack core genes typically involved in particle production. This is not completely unexpected given that no PDV replication takes place in the caterpillar. Particle production is confined to the wasp ovary where viral DNAs are generated from proviral copies maintained within the wasp genome. We recently showed that the genes involved in bracovirus particle production reside within the wasp genome and are related to nudiviruses. In the present work we characterized genes involved in ichnovirus particle production by analyzing the components of purified Hyposoter didymator Ichnovirus particles by LC-MS/MS and studying their organization in the wasp genome. Their products are conserved among ichnovirus-associated wasps and constitute a specific set of proteins in the virosphere. Strikingly, these genes are clustered in specialized regions of the wasp genome which are amplified along with proviral DNA during virus particle replication, but are not packaged in the particles. Clearly our results show that ichnoviruses and bracoviruses particles originated from different viral entities, thus providing an example of convergent evolution where two groups of wasps have independently domesticated viruses to deliver genes into their hosts., Author Summary The polydnaviruses (PDVs) are a unique virus type used by an organism (a parasitic wasp) to manipulate the physiology of another organism (a lepidopteran host) in order to ensure successful parasitism. The evolutionary origin of these unusual viruses, found in ∼17,500 braconid wasps (Bracoviruses) and ∼15,000 ichneumonid wasps (Ichnoviruses), has been a major question for the last decade. We thus undertook an exclusive work aiming at investigating this origin via the characterization of genes encoding structural components for both types of PDVs. The present paper constitutes the first report on the identity and genome organisation of the viral machinery producing Ichnovirus virions. Our results strongly suggest that Ichnoviruses originated from a virus belonging to a group as yet uncharacterized that integrated its genome into that of an ichneumonid wasp ancestor. More importantly, our results demonstrate that the ancestor of Ichnoviruses differs from that of Bracoviruses, which originated from a nudivirus. We have now identified, for the two types of PDVs, the non packaged viral genes and their products involved in producing particles injected into the host during oviposition. Together, these data provide an example of convergent evolution where different groups of wasps have independently domesticated viruses to deliver genes into their hosts.

Published
2010
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7. Members of the Hyposoter didymator Ichnovirus repeat element gene family are differentially expressed in Spodoptera frugiperda

Author

L. Galibert, Janick Rocher, Philippe Fournier, Anne-Nathalie Volkoff, M. Barat-Houari, Gérard Devauchelle, François Cousserans, Pierre Cérutti, Biologie Intégrative et Virologie des Insectes [Univ. de Montpellier II] (BIVI), Université Montpellier 2 - Sciences et Techniques (UM2)-Institut National de la Recherche Agronomique (INRA), Saint-Christol (SAINT-CHRISTOL), Institut National de la Recherche Agronomique (INRA), and Centre National de la Recherche Scientifique (CNRS)

Subjects
Gene Expression Regulation, Viral, food.ingredient, expression génique, Genes, Viral, Transcription, Genetic, Virologie, virus entomopathogène, viruses, Molecular Sequence Data, Wasps, Gene Dosage, Insect Viruses, virus, Spodoptera, Genome, Gene dosage, lcsh:Infectious and parasitic diseases, HYPOSOTER DIDYMATOR, Viral Proteins, food, Transcription (biology), Virology, Animals, BIOLOGIE DU DEVELOPPEMENT, Gene family, lcsh:RC109-216, Amino Acid Sequence, Peptide sequence, Gene, SPODOPTERA FRUGIPERDA, Repetitive Sequences, Nucleic Acid, Genetics, biology, Research, fungi, BIOLOGIE MOLECULAIRE, biology.organism_classification, Molecular biology, Infectious Diseases, Polydnaviridae, Larva, Multigene Family, [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology, Ichnovirus
Abstract

Background The abundance and the conservation of the repeated element (rep) genes in Ichnoviruses genomes suggest that this gene family plays an important role in viral cycles. In the Ichnovirus associated with the wasp Hyposoter didymator, named HdIV, 10 rep genes were identified to date. In this work, we report a relative quantitative transcription study of these HdIV rep genes in several tissues of the lepidopteran host Spodoptera frugiperda as well as in the H. didymator wasps. Results The data obtained in this work indicate that, in the early phases of infection (24 hours), HdIV rep genes each display different levels of transcripts in parasitized 2nd instar or HdIV-injected last instar S. frugiperda larvae. Only one, rep1, is significantly transcribed in female wasps. Transcript levels of the HdIV rep genes were found as not correlated to their copy number in HdIV genome. Our results also show that HdIV rep genes display different tissue specificity, and that they are primarily transcribed in S. frugiperda fat body and cuticular epithelium. Conclusion This work is the first quantitative analysis of transcription of the ichnovirus rep gene family, and the first investigation on a correlation between transcript levels and gene copy numbers in Ichnoviruses. Our data indicate that, despite similar gene copy numbers, not all the members of this gene family are significantly transcribed 24 hours after infection in lepidopteran larvae. Additionally, our data show that, as opposed to other described HdIV genes, rep genes are little transcribed in hemocytes, thus suggesting that they are not directly associated with the disruption of the immune response but rather involved in other physiological alterations of the infected lepidopteran larva.

Published
2006
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8. Egg and larval parasitoids of the beet armyworm Spodoptera exigua on maize in Turkey

Author

Serpil Kornoşor, Erdal Sertkaya, Ahmet Sami Bayram, and Çukurova Üniversitesi

Subjects
Meteorus ictericus, education.field_of_study, biology, Parasitoids, Population, Hyposoter didymator, Parasitism, Plant Science, Chelonus obscuratus, biology.organism_classification, Microplitis spp, Parasitoid, Lepidoptera genitalia, Crop, Agronomy, Beet armyworm, Insect Science, Spodoptera exigua, Sinophorus xanthostomus, PEST analysis, Apanteles, education
Abstract

The present study was conducted to determine egg and larval parasitoids of the beet armyworm Spodoptera exigua Hübner (Lepidoptera: Noctuidae), which is an important but sporadic pest in Turkey. High beet armyworm population levels were recently observed in fields of first and second crop maize in the southeast Mediterranean region of Turkey. The parasitoid species complex and its impact on the pest were analyzed in a 4-year study in first and second crop maize. The braconid larval parasitoids Microplitis rufiventris Kokujev, M. tuberculifer Wesmael, Meteorus ictericus Nees, Chelonus obscuratus (Herrich Schäffer) (an egg-larval parasitoid), Apanteles ruficrus (Haliday); the ichneumonid larval parasitoids Hyposoter didymator (Thunberg) and Sinophorus xanthostomus Gravenhorst; and the egg parasitoid Trichogramma evanescens (Westwood) were found to be the natural enemies attacking the pest. Among the parasitoid species the solitary endoparasitoid H. didymator was the most prevalent species, being reared from 40.5% of the parasitized larvae found. Higher parasitism rates were recorded on first crop than on second crop maize in every year. Possible reasons for this difference in larval parasitism between two growing seasons include lower population of the pest and reduced insecticide applications in first crop maize fields which permitted higher parasitism. However, parasitoid activity was insufficient to counterbalance the population growth of the pest on subsequent second crop maize.

Published
2004

9. Corrigendum to 'Venom gland extract is not required for successful parasitism in the polydnavirus-associated endoparasitoid Hyposoter didymator (Hym. Ichneumonidae) despite the presence of numerous novel and conserved venom proteins' [Insect Biochem. Mol. Biol. 43 (2013), 292–307]

Author

Tristan Dorémus, Dominique Colinet, Marylène Poirié, Eric Wajnberg, Julie Poulain, Véronique Jouan, Carole Azéma-Dossat, Serge Urbach, Edith Demettre, François Cousserans, Anne-Nathalie Volkoff, Jean-Luc Gatti, Isabelle Darboux, Marc Ravallec, and Jean-Michel Escoubas

Subjects
biology, Polydnavirus, media_common.quotation_subject, Parasitism, Venom, Insect, Venom gland, biology.organism_classification, Biochemistry, Hyposoter didymator, Ichneumonidae, Insect Science, Botany, Mole, Molecular Biology, media_common
Published
2014
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