1. Intestinal metabolomics of juvenile lenok (Brachymystax lenok) in response to heat stress
- Author
-
Yucen Bai, Xiaofei Yang, Bo Cheng, Yan Chen, Shaogang Xu, and Yang Liu
- Subjects
Erucic Acids ,Proline ,Physiology ,Glutamine ,Phosphorylcholine ,Phenylalanine ,Zoology ,Lenok ,Aquatic Science ,Biochemistry ,Methionine ,Metabolomics ,Brachymystax lenok ,Leucine ,Tandem Mass Spectrometry ,Animals ,Juvenile ,Histidine ,Oxylipins ,12-Hydroxy-5,8,10,14-eicosatetraenoic Acid ,Aspartic Acid ,biology ,Palmitoylcarnitine ,Tryptophan ,General Medicine ,biology.organism_classification ,Heat stress ,Intestines ,Glucose ,Phosphatidylcholines ,Lactates ,Tyrosine ,lipids (amino acids, peptides, and proteins) ,Acetylcarnitine ,Salmonidae ,Heat-Shock Response - Abstract
Changes in the metabolic profile within the intestine of lenok (Brachymystax lenok) when challenged to acute and lethal heat stress (HS) are studied using no-target HPLC-MS/MS metabonomic analysis. Of 51 differentially expressed metabolites identified in response to HS, 34 occurred in the positive ion mode and 17 in negative ion mode (VIP > 1, P < 0.05). Changes in metabolites (i.e. alpha-D-glucose, stachyose and L-lactate) related to carbohydrate and glycolysis are identified in HS-treated lenok. Fatty acid β-oxidation in HS-treated lenok was inhibited by accumulation of acetyl carnitine, palmitoylcarnitine, carnitine, and erucic acid. Many amino acids (L-tryptophan, D-proline, L-leucine, L-phenylalanine, L-aspartate, L-tyrosine, L-methionine, L-histidine and L-glutamine) decreased to support energy demands in HS-treated lenok. Oxidative damage in HS-treated lenok was indicated by decreased glycerophospholipid metabolites (i.e. glycerophosphocholine, 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphoethanolamine, 1-palmitoyl-sn-glycero-3-phosphocholine, 1-stearoyl-2-oleoyl-sn-glycero-3-phosphocholine, and 1, 2-dioleoyl-sn-glycero-3-phosphatidylcholine), and increased oxylipin production (12-HETE and 9R, 10S-EpOME). Oxidative stress increased formation of eicosanoids and dicarboxylic acids, overwhelming the mitochondrial β-oxidation pathway, while minor oxidative pathways (omega-oxidation and peroxisomal beta-oxidation) were likely to be activated in HS-treated lenok.
- Published
- 2022