1. PD-L1 Expression and CD8+ T-cell Infiltrate are Associated with Clinical Progression in Patients with Node-positive Prostate Cancer
- Author
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Paola M.V. Rancoita, Laetitia Lacroix, Catherine Sautès-Fridman, Wolf H. Fridman, Massimo Freschi, Etienne Becht, Yann Vano, Alberto Briganti, Claudio Doglioni, Roberta Lucianò, Nicola Fossati, Matteo Bellone, Julien Calderaro, Francesco Montorsi, Tiffany Guédet, Florent Petitprez, Petitprez, Florent, Fossati, Nicola, Vano, Yann, Freschi, Massimo, Becht, Etienne, Luciano, Roberta, Calderaro, Julien, Guedet, Tiffany, Lacroix, Laetitia, Rancoita, PAOLA MARIA VITTORIA, Montorsi, Francesco, Fridman, Wolf Herman, Sautes Fridman, Catherine, Briganti, Alberto, Doglioni, Claudio, and Bellone, Matteo
- Subjects
PD-L1 ,0301 basic medicine ,PCA3 ,Oncology ,medicine.medical_specialty ,Urology ,medicine.medical_treatment ,Androgen deprivation therapy ,Lymph node metastasi ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Recurrence ,Internal medicine ,T lymphocyte ,Medicine ,Lymph node ,CD20 ,B lymphocyte ,Progression ,biology ,business.industry ,Prostatectomy ,Biomarker ,medicine.disease ,Radical prostatectomy ,Immune checkpoint ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,biology.protein ,business - Abstract
Prostate cancer (PCa) patients with lymph node invasion at radical prostatectomy are at higher risk of tumor recurrence and receive immediate androgen deprivation therapy (ADT). While approximately 30% of these patients do not experience recurrence, others experience disease recurrence despite ADT, and currently no biomarkers can accurately identify them. We analyzed tumors from 51 patients with node-positive prostate cancer using immunohistochemistry to investigate whether expression of the immune checkpoint ligand PD-L1 by tumor cells or the density of CD8+ or CD20+ cells are associated with clinical progression. Patients with at least 1% PD-L1+ tumor cells had shorter metastasis-free survival than those with PD-L1- tumors (p =0.008, log-rank test). Univariate Cox regression showed that patients with PD-L1+ tumors had almost four times the risk of experiencing distant metastases than those with PD-L1- tumors (hazard ratio 3.90). In addition, we found that PD-L1 expression was significantly associated with CD8+ T-cell density, but not with CD20+ B-cell density. While these results need to be confirmed in larger studies, they show that PD-L1 and CD8 may be used as biomarkers for node-positive patients at high risk of progression. The study also provides a rationale for selecting patients with node-positive PCa who might benefit the most from adjuvant immunotherapies. Patient summary: None of the available biomarkers can identify node-positive prostate cancer that will recur after surgery. We found that expression of PD-L1 by tumor cells and a high density of CD8+ T cells in tumor are associated with a higher risk of clinical progression in men with node-positive prostate cancer. Biological markers predicting tumor recurrence in node-positive prostate cancer (PCa) are currently lacking. By investigating formalin-fixed paraffin-embedded tissue sections from 51 patients with node-positive PCa, we found that patients with tumors expressing PD-L1 and/or with a higher frequency of intratumor CD8+ T cells were at the highest risk of developing PCa metastasis after surgery. These results may open new frontiers for the use of adjuvant immunotherapy in this subset of node-positive patients.
- Published
- 2019
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