1. LM-GlycomeAtlas Ver. 1.0: A Novel Visualization Tool for Lectin Microarray-Based Glycomic Profiles of Mouse Tissue Sections
- Author
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Kozue Hagiwara, Yoshinori Suzuki, Chiaki Nagai-Okatani, Atsushi Kuno, Misugi Nagai, Kiyoko F. Aoki-Kinoshita, Kiyohiko Angata, Noriaki Fujita, Shuichi Kakuda, Katsue Kiyohara, and Takashi Sato
- Subjects
Male ,Protein glycosylation ,formalin-fixed paraffin-embedded tissue section ,Glycan ,Glycosylation ,Microarray ,Protein Array Analysis ,Pharmaceutical Science ,Mouse tissue ,Computational biology ,Biology ,Article ,Analytical Chemistry ,lcsh:QD241-441 ,Mice ,User-Computer Interface ,03 medical and health sciences ,0302 clinical medicine ,lectin microarray ,lcsh:Organic chemistry ,Lectins ,Drug Discovery ,Animals ,Physical and Theoretical Chemistry ,Glycomics ,030304 developmental biology ,Laser capture microdissection ,0303 health sciences ,Organic Chemistry ,Lectin ,mouse tissue glycome mapping ,Glycome ,Visualization ,Organ Specificity ,Chemistry (miscellaneous) ,030220 oncology & carcinogenesis ,biology.protein ,Molecular Medicine ,laser microdissection ,Microdissection ,Software - Abstract
For the effective discovery of the biological roles and disease-specific alterations concerning protein glycosylation in tissue samples, it is important to know beforehand the quantitative and qualitative variations of glycan structures expressed in various types of cells, sites, and tissues. To this end, we used laser microdissection-assisted lectin microarray (LMA) to establish a simple and reproducible method for high-throughput and in-depth glycomic profiling of formalin-fixed paraffin-embedded tissue sections. Using this &ldquo, tissue glycome mapping&rdquo, approach, we present 234 glycomic profiling data obtained from nine tissue sections (pancreas, heart, lung, thymus, gallbladder, stomach, small intestine, colon, and skin) of two 8-week-old male C57BL/6J mice. We provided this LMA-based dataset in the similar interface as that of GlycomeAtlas, a previously developed tool for mass spectrometry-based tissue glycomic profiling, allowing easy comparison of the two types of data. This online tool, called &ldquo, LM-GlycomeAtlas&rdquo, allows users to visualize the LMA-based tissue glycomic profiling data associated with the sample information as an atlas. Since the present dataset allows the comparison of glycomic profiles, it will facilitate the evaluation of site- and tissue-specific glycosylation patterns. Taking advantage of its extensibility, this tool will continue to be updated with the expansion of deposited data.
- Published
- 2019