1. LncRNA H19 induces immune dysregulation of BMMSCs, at least partly, by inhibiting IL-2 production
- Author
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Xiuxia Luo, Zhihua Yin, Yazhi Wei, Xinpeng Chen, Yidou Tangzhou, Huijie Jin, Dai Liping, and Hualin Sun
- Subjects
0301 basic medicine ,Apoptosis ,medicine.disease_cause ,T-Lymphocytes, Regulatory ,Biochemistry ,chemistry.chemical_compound ,0302 clinical medicine ,Cell Movement ,Lupus Erythematosus, Systemic ,Genetics (clinical) ,Cells, Cultured ,biology ,medicine.diagnostic_test ,Cell Differentiation ,female genital diseases and pregnancy complications ,lncRNA H19 ,030220 oncology & carcinogenesis ,embryonic structures ,Molecular Medicine ,RNA, Long Noncoding ,Disease Susceptibility ,Antibody ,Research Article ,Immune regulation ,RM1-950 ,QD415-436 ,Flow cytometry ,Immunomodulation ,03 medical and health sciences ,Systemic lupus erythematosus ,Downregulation and upregulation ,Genetics ,medicine ,Humans ,DAPI ,Molecular Biology ,IL-2 ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Immune dysregulation ,Molecular medicine ,Coculture Techniques ,030104 developmental biology ,chemistry ,Gene Expression Regulation ,Case-Control Studies ,biology.protein ,Cancer research ,Leukocytes, Mononuclear ,Interleukin-2 ,Therapeutics. Pharmacology ,Biomarkers - Abstract
Background Systemic lupus erythematosus (SLE) is a representative systemic autoimmune disease. LncRNA H19 has been identified to participate in various biological processes in human diseases. However, the role of H19 in SLE remains unclear. Methods In this study, we first examined H19 expression in SLE patients by RT-qPCR and found that H19 expression was significantly upregulated in the serum and bone marrow-derived mesenchymal stem cells (BMMSCs) of SLE patients and positively associated with SLE disease activity index. We then performed gain-of-function and loss-of-function using mimic-H19 (H19-OE) and inhibitor-H19 (H19-KD) to examine the effects of H19 on BMMSC differentiation, proliferation, migration, and apoptosis using flow cytometry, DAPI staining, and migration and apoptosis assays. Results The results showed that H19 inhibited proliferation and migration but promoted apoptosis of BMMSCs, interfered with BMMSCs-mediated Treg cell proliferation and differentiation, and regulated BMMSCs-mediated Tfh/Treg cell balance. Dual-luciferase reporter assay confirmed the in silico prediction of interaction between H19 and IL-2. Furthermore, RT-qPCR showed that H19 directly inhibited IL-2 transcription in BMMSCs. ELISA showed that both active and total IL-2 protein levels were significantly lower in SLE BMMSCs. More importantly, we found that IL-2 significantly enhanced H19-OE-induced Treg cell differentiation and migration of BMMSCs, and these effects were reversed by anti-IL-2 antibody. Conclusion Overall, our study indicates that LncRNA H19 induces immune dysregulation of BMMSCs, at least partly, by inhibiting IL-2 production and might be a novel therapeutic target for SLE.
- Published
- 2021