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Your search keyword '"Hong, Xu"' showing total 4 results
4 results on '"Hong, Xu"'

1. Extracellular vesicle-derived long non-coding RNA as circulating biomarkers for endometriosis

Author

Yeping Yang, Bingxin Yang, Shan Shan, Feng Sun, Jun-Yu Zhang, Hong Xu, Yang Yisai, Jiang Jilan, and Yu Lin

Subjects
Endometriosis, Exosome, law.invention, Extracellular Vesicles, law, medicine, Humans, KEGG, Polymerase chain reaction, Messenger RNA, Sequence Analysis, RNA, business.industry, Obstetrics and Gynecology, RNA, Extracellular vesicle, medicine.disease, ROC Curve, Reproductive Medicine, Cancer research, Biomarker (medicine), Female, RNA, Long Noncoding, business, Biomarkers, Developmental Biology
Abstract

Research question Whether extracellular vesicle-derived lncRNAs could serve as promising circulating biomarkers for endometriosis? Design To obtain novel diagnostic markers, we enrolled 85 patients with endometriosis as the endometriosis group and 86 unaffected participants as the control group. RNA sequencing was performed to identify exosomal long non-coding RNAs (lncRNAs) that are differentially expressed between women with endometriosis (n=5) and unaffected participants (n=6). Messenger RNA and lncRNA sequences of the plasma exosomes were analyzed using Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses. LncRNA expression levels were further validated using quantitative reverse transcriptase polymerase chain reaction. Moreover, receiver operating characteristic curve analysis was performed to determine the diagnostic value of candidate lncRNAs. Clinical features were correlated to the expression levels of candidate lncRNAs. Results We found that 210 lncRNAs were significantly dysregulated; among these, expression of LINC01569, RP3-399L15.2, FAM138B, and CH507-513H4.6 was significantly decreased, whereas expression of RP11-326N17.2, KLHL7-AS1, and MIR548XHG was increased, in the plasma of patients with endometriosis. Combined expression level of RP3-399L15.2 and CH507-513H4.6 was used to distinguish patients with endometriosis from control participants; the results revealed a sensitivity of 80.00% and specificity of 85.45% at the cutoff point, and an area under the ROC curve of 0.9045. Our findings demonstrated the potential of these two lncRNAs as diagnostic biomarkers for endometriosis. Moreover, CH507-513H4.6 alone may be useful to detect early-stage endometriosis lesions. Conclusions The combination of RP3-399L15.2 and CH507-513H4.6 can be potential candidates for endometriosis biomarkers.

Published
2022

2. Early growth response 1 reduction in peripheral blood involving condylar subchondral bone loss.

Author

Zhang, Hong‐Yun, Liu, Qian, Yang, Hong‐Xu, Shi, Li‐Qiang, Wang, Pei, Xie, Mian‐Jiao, Liu, Jin‐Qiang, Xu, Xiao‐Jie, Liu, Xiao‐Dong, Yu, Shi‐Bin, Jiao, Kai, Zhang, Mian, Xuan, Shi‐Jie, Xu, Yi‐Fei, Zhang, Xuan, Liu, Yi‐Fan, Zhang, Jing, and Wang, Mei‐Qing

Subjects
OSTEOARTHRITIS diagnosis, ANIMAL experimentation, GENE expression, LEUCOCYTES, MALOCCLUSION, MEMBRANE proteins, MESSENGER RNA, OSTEOARTHRITIS, RATS, TEMPOROMANDIBULAR disorders, TRANSCRIPTION factors, SYMPTOMS
Abstract

Objectives: To detect whether early growth response 1 (EGR1) in peripheral blood leucocytes (PBLs) indicates temporomandibular joint (TMJ) osteoarthritis (OA) lesions. Materials and Methods: Egr1 mRNA expression levels in PBLs were detected in eight malocclusion patients without temporomandibular disorder (TMD) signs and 16 malocclusion patients with clinical TMD signs with (eight) or without (eight) imaging signs of TMJ OA. Twelve 6‐week‐old rats were randomized to a control group and a unilateral anterior crossbite (UAC) group and were sampled at 4 weeks. The Egr1 mRNA expression levels in PBLs and protein expression levels in different orofacial tissues were measured. Results: Patients with TMD signs with/without TMJ OA diagnosis showed lower Egr1 mRNA expression levels in PBLs than patients without TMD signs. The lower Egr1 mRNA expression was also found in the PBLs of UAC rats, which were induced to exhibit early histo‐morphological signs of TMJ OA lesions. In subchondral bone of UAC rats, EGR1 protein expression was decreased, co‐localization of EGR1 with osterix or dentin matrix protein‐1 was identified, and the number of EGR1 and osterix double‐positive cells was reduced (all p < .05). Conclusion: Egr1 reduction in PBLs potentially indicates subchondral bone OA lesions at an early stage. [ABSTRACT FROM AUTHOR]

Published
2019
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3. Catabolic changes of rat temporomandibular joint discs induced by unilateral anterior crossbite.

Author

Zhang, Hong‐Yun, Xie, Mian‐Jiao, Yang, Hong‐Xu, Liu, Xin, Ren, Hao‐Tian, Zhang, Mian, Lu, Lei, Liu, Xiao‐Dong, Zhang, Jing, and Wang, Mei‐Qing

Subjects
ALKALINE phosphatase, ANIMAL experimentation, BIOMARKERS, BONE growth, CHONDROGENESIS, COLLAGEN, EXTRACELLULAR space, FIBRONECTINS, GENE expression, GLYCOPROTEINS, MESSENGER RNA, DENTAL occlusion, RATS, TEMPOROMANDIBULAR disorders, TRANSCRIPTION factors, WOUND healing, VASCULAR endothelial growth factors, DISEASE complications, DISEASE risk factors
Abstract

Summary: Background: The temporomandibular joint (TMJ) disc plays a role in joint movement and in load absorbance and distribution. An experimental unilateral anterior crossbite (UAC) prosthesis induces mandibular condylar cartilage degeneration in rats. However, the changes in the articular disc are still unknown. Objective: To describe changes in the TMJ discs of UAC rats. Methods: The discs of fifty‐four Sprague‐Dawley rats, equally distributed into a UAC group and an age‐matched sham‐operated control group at 4, 12 and 20 weeks (n = 9), were evaluated by gross and histomorphological observation and by detection at the mRNA or protein expression levels of the markers related to the matrix elements. Results: No macro‐ or micro‐morphological differences were observed between groups. However, there were catabolic degradative changes at the molecular level in the UAC group, showing a significant reduction in the mRNA and/or protein expression levels of many molecules. The reduction became worse with time (P < 0.05). The reduced molecules included: (a) those related to the extracellular matrix, such as type I collagen, decorin and fibromodulin; (b) those related to chondrogenesis, such as type II collagen and aggrecan; and (c) those related to osteogenesis, such as alkaline phosphatase and runt‐related transcription factor 2. The mRNA expression of vascular endothelial growth factor did not change. In contrast, fibronectin, which can promote wound healing, and its N‐terminal fragment, which can induce cartilage degradation, were accumulated (P < 0.05). Conclusion: TMJ discs were stimulated to catabolic changes by the aberrant dental occlusion and seemed to go to inanimate with time. [ABSTRACT FROM AUTHOR]

Published
2019
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4. The significance of Pax2 expression in the ureter epithelium of children with vesicoureteric reflux

Author

Wei Guo, Yibing Zheng, Xin Zhang, Hong Xu, Yihui Zhai, Jing Chen, Jing Xu, and Qian Shen

Subjects
Male, medicine.medical_specialty, animal structures, Urinary system, PAX2, Urology, Apoptosis, Biology, urologic and male genital diseases, Vesicoureteral reflux, Pathology and Forensic Medicine, Epigenesis, Genetic, Ureter, medicine, Humans, DNA (Cytosine-5-)-Methyltransferases, RNA, Messenger, Child, Vesico-Ureteral Reflux, Messenger RNA, urogenital system, PAX2 Transcription Factor, Reflux, Infant, DNA Methylation, medicine.disease, Up-Regulation, medicine.anatomical_structure, Child, Preschool, embryonic structures, DNA methylation, Immunohistochemistry, Female, sense organs, Urothelium, Biomarkers
Abstract

Vesicoureteral reflux (VUR) is the retrograde passage of urine from the bladder to the urinary tract; this leads to renal scarring and end-stage renal disease in children. Pax2 is a nuclear transcription factor that is involved in urinary system development. We measured the expression of Pax2 in the ureters of 47 patients with VUR. The messenger RNA expression and the protein level of Pax2 were significantly increased in patients with VUR, suggesting a correlation with VUR. Further studies demonstrated that Pax2 was hypomethylated, and Dnmt3a messenger RNA expression was significantly lower than in the control group. We speculate that the low level of Dnmt2a might decrease PAX2 gene methylation and up-regulate the Pax2 protein. The high level of Pax2 might be related to cellular apoptosis and functional lesions in ureters. In conclusion, our results revealed that the level of Pax2 is correlated with VUR; thus, Pax2 represents a possible target for VUR therapy.

Published
2014

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