1. Fecal Microbiota Restoration Modulates the Microbiome in Inflammation-Driven Colorectal Cancer.
- Author
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Gates, Travis J, Yuan, Ce, Shetty, Mihir, Kaiser, Thomas, Nelson, Andrew C, Chauhan, Aastha, Starr, Timothy K, Staley, Christopher, and Subramanian, Subbaya
- Subjects
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BIOLOGICAL models , *BIOMARKERS , *CARCINOGENS , *INFLAMMATION , *GUT microbiome , *ANIMAL experimentation , *ACUTE phase proteins , *COLORECTAL cancer , *RESEARCH funding , *DEXTRAN , *LONGITUDINAL method , *ANTIBIOTICS , *BACTERIA , *DISEASE risk factors , *DISEASE complications - Abstract
Simple Summary: Inflammation of the colon (colitis) can increase the risk of developing colorectal cancers. Gut microbiota may play a role in the development of this cancer. One potential way to prevent this cancer is to manipulate the community of bacteria in the colon. In a study using mice, we found that transferring the bacteria from the bedding of healthy mice to those with colitis increased the presence of Akkermansia, which may help reduce inflammation in the colon. Meanwhile, untreated mice had an increase in other types of bacteria, such as Anaeroplasma and Alistipes, which may contribute to inflammation. These findings suggest that certain types of bacteria may help reduce inflammation in the colon and prevent cancer. Chronic inflammation of the colon (colitis) is a known risk factor for inflammatory-driven colorectal cancers (id-CRCs), and intestinal microbiota has been implicated in the etiology of id-CRCs. Manipulation of the microbiome is a clinically viable therapeutic approach to limiting id-CRCs. To understand the microbiome changes that occur over time in id-CRCs, we used a mouse model of id-CRCs with the treatment of azoxymethane (AOM) and dextran sodium sulfate (DSS) and measured the microbiome over time. We included cohorts where the microbiome was restored using cage bedding swapping and where the microbiome was depleted using antibiotics to compare to untreated animals. We identified consistent increases in Akkermansia in mice receiving horizontal microbiome transfer (HMT) via cage bedding swapping, while the control cohort had consistent longitudinal increases in Anaeroplasma and Alistipes. Additionally, fecal lipocalin-2 (Lcn-2), a marker of intestinal inflammation, was elevated in unrestored animals compared to restored and antibiotic-treated counterparts following HMT. These observations suggest a potential role for Akkermansia, Anaeroplasma, and Alistipes in regulating colonic inflammation in id-CRCs. [ABSTRACT FROM AUTHOR]
- Published
- 2023
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