Search

Abstract: Biomass pretreatment is essential to overcome recalcitrance of lignocellulose for ethanol production. In the present study we pretreated giant reed (Arundo donax L.), a perennial, rhizomatous lignocellulosic grass with dilute oxalic acid. The effects of temperature (170–190 °C), acid loading (2–10% w/w) and reaction time (15–40 min) were handled as a single parameter, combined severity. We explored the change in hemicellulose, cellulose and lignin composition following pretreatment and glucan conversion after enzymatic hydrolysis of the solid residue. Two different yeast strains, Scheffersomyces (Pichia) stipitis CBS 6054, which is a native xylose and cellobiose fermenter, and Saccharomyces carlsbergensis FPL-450, which does not ferment xylose or cellobiose, were used along with commercial cellulolytic enzymes in simultaneous saccharification and fermentation (SSF). S. carlsbergensis attained a maximum ethanol concentration of 15.9 g/l after 48 h at pH 5.0, while S. stipitis, at the same condition, took 96 h to reach a similar ethanol value; increasing the pH to 6.0 reduced the S. stipitis lag phase and attained 18.0 g/l of ethanol within 72 h. [Copyright &y& Elsevier]

Within the project "BIOSEA" funded by the Italian Ministry of Agriculture and Forestry, a preliminary laboratory test was conducted to assess the variability for cold tolerance during germination in 30 cultivars of biomass sorghum, among fiber and sweet types. Seed germination (%) and mean germination time (MGT) were examined at seven constant temperatures (from 8 °C to 35 °C) and base temperature (Tb) and thermal time (θT) for 50% germination were calculated. A wide genetic diversity in the germination response of sorghum was ascertained at 8 °C (CV 45%) and 10 °C (CV 25.4%). At 8 °C, in cultivars of 'Padana 4', 'PR811F', 'PSE24213', 'PR849' and 'Zerberus', seed germination exceeded 80%. Seeds of 'Zerberus' were also the fastest, requiring less than 13 days for final germination at this low temperature. Great differences were found in Tb and θT among cultivars. Tb varied between 7.44 °C ('PR811F') and 13.48 °C ('Nectar'). Thermal time (θT) was, on average, 24.09 °Cd−1, and ranged between 16.62 ('Nectar') and 33.42 °Cd−1 ('PSE24213'). The best combination of the two germination parameters (i.e., low Tb and θT) corresponded to 'Zerberus', 'Sucrosorgo 506', 'Jumbo' and 'PR811F'. Accordingly, these cultivars are more tolerant to cold stress during germination and, thus, more adapt to early spring sowings in Mediterranean areas (March-April). Cultivars 'PR811F' (fiber type) and 'Sucrosorgo 506' (sweet type) also combine high cold tolerance with good productivity in terms of final dry biomass, as assessed in open-field conditions (late spring sowing). The genetic variation in the germination response to a low temperature is useful for the identification of genotypes of sorghum suitable to early sowings in semi-arid areas. Selection within existing cultivars for cold tolerance during germination may also contribute to the expansion of biomass sorghum into cooler cultivation areas, such as those of Northern Europe, which are less suitable to this warm season crop. [ABSTRACT FROM AUTHOR]

Side by side biomass productivities, harvesting time (autumn vs. winter) and frequency (annual vs. biennial) of three perennial grasses were compared under northern and southern Mediterranean climates. Miscanthus ( Miscanthus × giganteus Greef et Deu.) was compared to giant reed ( Arundo donax L.) in Catania (37°24′N, 15°03′E), and to switchgrass ( Panicum virgatum L.) in Bologna (44°55'N, 11°45′E). Generally, giant reed produced about 50% higher biomass than miscanthus in Catania. Miscanthus almost halved biomass productivity in the driest year, while giant reed reduced biomass yield by only 30% under the same conditions. In Bologna, miscanthus and switchgrass produced similar amounts of cumulative biomass over 6 years. Switchgrass kept more stable annual yields than miscanthus, which, however, evidenced a higher potential under favorable climate conditions. Autumn harvest significantly reduced biomass productivity and quality (moisture content, ashes, cellulose and hemicellulose), particularly under South Mediterranean climate. In Catania, autumn biomass was 50% (giant reed) to 85% (miscanthus) lower than winter biomass, while in Bologna, autumn cut reduced switchgrass yield by 20%. Biennial harvesting resulted in almost 40% lower cumulative biomass yield than annual cut, after 6 years. [ABSTRACT FROM AUTHOR]

Kenaf (Hibiscus cannabinus L.) is a warm-season annual plant grown in the past for the bast fiber. In the last decades, this crop has been re-evaluated for many alternative industrial uses of its biomass (i.e., production of biofuel, biocomposite materials, bioproducts). In this paper, the interaction effects of sowing time (May and June) and plant population (P20 and P40, 20 and 40 plants m-2, respectively) on yield and some biomass parameters were analysed in two cultivars of kenaf (Tainung 2-TAI, and Everglades 41-EVER), in a two-year experiment in semi-arid environment, under irrigation. The aim of this paper was to assess the potential of this crop as an energy or industrial feedstock, in south Mediterranean environment where was never cultivated. Overall, plants from late sowing (June) were smaller and with less node, those at high plant density (P40) were thinner. In both seasons, plants of TAI were taller than those of EVER. Plant weight was greater in P20, and an overall significant negative correlation was found between plant weight and actual plant density (r = −0.76 **). The shift of sowing time reduced significantly the final dry biomass. Crop productivity did not change with plant density, however a higher percent bark corresponded to P40 in both years, cultivars, and sowing times, A simple model was developed to simulate and predict the course of LAI and dry biomass (AGB) with time, using field dataset of 2004, for calibration, and dataset of 2005, for validation. The minor response of LAI to plant population in 2005 led to a low accuracy of model approximation. This result suggests that other variables that are not considered in the model, may influence LAI. Nevertheless, the overall AGB model approximation of observed value was accurate. Concluding, the results revealed that biomass productivity is more influenced by sowing time than by plant density. However, plant population has been found to regulate the proportion of bark/core layers, with important implications for industrial (energy and pulp production) purposes. Further improvements on the simple model developed, e.g., including soil water nitrogen balances submodels, may be done, in order to explore the possibility of using the model under different conditions of soil water content and nitrogen fertilization. • Sowing time and plant density effects were studied in two cultivars of kenaf. • Dry biomass yield was significantly reduced shifting sowing from May to June. • Dry biomass did not change with plant density (20 or 40 plants m-2). • The % incidence of bark on core weight was greater in plants at higher density. • A simple model was developed to simulate and predict LAI and biomass in kenaf. [ABSTRACT FROM AUTHOR]