1. Combining Metabolic Engineering and Electrocatalysis: Application to the Production of Polyamides from Sugar.
- Author
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Suastegui, Miguel, Matthiesen, John E., Carraher, Jack M., Hernandez, Nacu, Rodriguez Quiroz, Natalia, Okerlund, Adam, Cochran, Eric W., Shao, Zengyi, and Tessonnier, Jean-Philippe
- Subjects
ELECTROCATALYSIS ,POLYAMIDES ,MUCONIC acid ,BIOMASS conversion ,BIOMASS energy ,SPECIALTY chemicals ,SACCHAROMYCES cerevisiae ,HEXAMETHYLENEDIAMINE - Abstract
Biorefineries aim to convert biomass into a spectrum of products ranging from biofuels to specialty chemicals. To achieve economically sustainable conversion, it is crucial to streamline the catalytic and downstream processing steps. In this work, a route that combines bio- and electrocatalysis to convert glucose into bio-based unsaturated nylon-6,6 is reported. An engineered strain of Saccharomyces cerevisiae was used as the initial biocatalyst for the conversion of glucose into muconic acid, with the highest reported muconic acid titer of 559.5 mg L
−1 in yeast. Without any separation, muconic acid was further electrocatalytically hydrogenated to 3-hexenedioic acid in 94 % yield despite the presence of biogenic impurities. Bio-based unsaturated nylon-6,6 (unsaturated polyamide-6,6) was finally obtained by polymerization of 3-hexenedioic acid with hexamethylenediamine. [ABSTRACT FROM AUTHOR]- Published
- 2016
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