1. Cell wall and cytoskeletal contributions in single cell biomechanics of Nicotiana tabacum.
- Author
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Ginsberg, Leah, McDonald, Robin, Qinchen Lin, Hendrickx, Rodinde, Spigolon, Giada, Ravichandran, Guruswami, Daraio, Chiara, and Roumeli, Eleftheria
- Subjects
TOBACCO ,CYTOSKELETON ,BIOMECHANICS ,ATOMIC force microscopy ,OSMOTIC pressure - Abstract
Studies on the mechanics of plant cells usually focus on understanding the effects of turgor pressure and properties of the cell wall (CW). While the functional roles of the underlying cytoskeleton have been studied, the extent to which it contributes to the mechanical properties of cells is not elucidated. Here, we study the contributions of the CW, microtubules (MTs) and actin filaments (AFs), in the mechanical properties of Nicotiana tabacum cells. We use a multiscale biomechanical assay comprised of atomic force microscopy and micro-indentation insolutions that (i) removeMTs andAFs and(ii) alter osmoticpressures in the cells. To compare measurements obtained by the two mechanical tests, we develop two generative statistical models to describe the cell's behaviour using one or both datasets. Our results illustrate that MTs and AFs contribute significantly to cell stiffness and dissipated energy, while confirming the dominant role of turgor pressure. [ABSTRACT FROM AUTHOR]
- Published
- 2022
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