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Ethnic value of many known plants are underexploited for medicinal application besides their proven traditional qualities. One such plant known for wound healing is Tridax procumbens. This plant has wound healing property and is commercially unexploited. Silver nanoparticle (Ag‐NP) were synthesized using this plant extracts using different solvents (methanol, ethyl acetate and aqueous), which exhibit resonance at 426, 424 and 418 nm, respectively. This plant‐mediated Ag‐NPs have strong anti‐bactericidal activity against Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Streptococcus pyogenes, Klebsiella pneumonia, Serratia marcescens and Bacillus subtilis with methanol extract. Further instance, elemental composition was confirmed by energy dispersive X‐ray analysis and particle size ranges were observed at 80–200 nm with spherical shape nanoparticles by scanning electron microscopy and transmission electron microscopy analysis. The biocompatibility of Ag‐NPs was assessed using fibroblast cell line (L929) by MTT assay with 109.35 µg IC50 value. The oxygen plasma treated and non‐treated bamboo spunlaced nonwoven fabrics were coated with the Ag‐NPs by exhaust method. Contact angle and water retention revealed significant difference in absorption ability of plasma treated fabric. Field emission scanning electron microscopy revealed the presence of Ag‐NPs in plasma coated fabrics. The fabricated cloth was studied for anti‐microbial and microbial penetration ability. [ABSTRACT FROM AUTHOR]