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In this study, Mo 3 Se 3 - single-chain atomic crystals (SCACs) with atomically small chain diameters of ∼0.6 nm, large surface areas, and mechanical flexibility were synthesized and investigated as an extracellular matrix (ECM)-mimicking scaffold material for tissue engineering applications. The proliferation of L-929 and MC3T3-E1 cell lines increased up to 268.4 ± 24.4% and 396.2 ± 8.1%, respectively, after 48 h of culturing with Mo 3 Se 3 - SCACs. More importantly, this extremely high proliferation was observed when the cells were treated with 200 μg mL -1 of Mo 3 Se 3 - SCACs, which is above the cytotoxic concentration of most nanomaterials reported earlier. An ECM-mimicking scaffold film prepared by coating Mo 3 Se 3 - SCACs on a glass substrate enabled the cells to adhere to the surface in a highly stretched manner at the initial stage of cell adhesion. Most cells cultured on the ECM-mimicking scaffold film remained alive; in contrast, a substantial number of cells cultured on glass substrates without the Mo 3 Se 3 - SCAC coating did not survive. This work not only proves the exceptional biocompatible and bioactive characteristics of the Mo 3 Se 3 - SCACs but also suggests that, as an ECM-mimicking scaffold material, Mo 3 Se 3 - SCACs can overcome several critical limitations of most other nanomaterials.